Lsi05G005280 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G005280
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionC2 domain-containing protein
Locationchr05: 6611316 .. 6612518 (-)
RNA-Seq ExpressionLsi05G005280
SyntenyLsi05G005280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCCAGTTGAGAAATTGGGTGTTTTAGAGGAAGAGATAAAGGAATGTGTTGTGGGAAATGAGAAGGAAGGAAAGGAAGTTGTTGGTGTTCTTGAGGTGTTTGTTCACCAAGCAAGAGATATTCATAATATATGTATTTATCATAAGCAAGATGTGTATGCAAGGCTTTCATTTACAACAGATCCTGAAAACTCTGTTTCCACCAAAACCATCAATGGTGGTGGGAGAAATCCTGTGTTCAATGACAGTGTTCATCTCGACGTTCGGTCAATCGATACTACGCTCAAATGCGAGATATGGATGCTGAGCAGGGTCAAGAATTATCTTGAAGATCAGTTGCTTGGTTTTGCTTTGATTCCTCTAACAGAAGTTGTTGCTGTCAATGGAAAGTTAGAGAAAGAATTCGGTCTTTCGTCGACCGATTTGTTCCATTCTCCCGCCGGGTTCGTGCAGTTATCGCTTTCGTATAATGGAGCTTCTCCAGAGGTCATGGCGATTCCGACCATTGAAAGAACTGTAGGGGTGGAGGATTCAGATTTAACCAAGTCACATCCAAGTGATTTGGACATGATCGAATTCCCTGATCCGAAGATTGCGAATGAAGATCAAATAATGGTGTCTGAGTATTTCGGCATCCCCTGCTCGAATTTGGATTCTGAATCTTCTGAGAGCTTAGCCATTTCTGAGGGTGAGAATCATGGCATTTCTGTGGTCGAAAGCTTTTCAATATCGAACGACAAGTCGAGTGAAGATTCGAAGATTGATTCTCCACCTAGTAATGTGCAAAATGATGCGGTTTCTTCACCGTCGTCGCCATCAGAAGCTCGAGCGAGATCAAAATGTACAACTCAAGAACATGTTTCAGCAGCAATTACCACAAGTGAGAAAGAACAAAATGGTGAAAATTGTGAGGTTAATGATTCTTCTAGTGAGGCAGCAATTGCCAATGACACAATTAAAAAGCCATTAGTTTCTGTGAGCATTGAGCCAGAAGACAAGGTAGTGCAACAAGACATTGTGGACATGTACATGAAAAGTATGCAACAATTCACAGAGTCATTGGCAAAGATGAAGCTACCTTTGGATATTGACACTAATGGCTCTCCAAGTTCAGCTACTTCGAGCTCCGACCTGAACTTACAATCGCCCAAAAACAACGTTTCGCGTGTGTTTTACGGGAGCAGGGCGTTCTTCTGA

mRNA sequence

ATGGCTTCCCCAGTTGAGAAATTGGGTGTTTTAGAGGAAGAGATAAAGGAATGTGTTGTGGGAAATGAGAAGGAAGGAAAGGAAGTTGTTGGTGTTCTTGAGGTGTTTGTTCACCAAGCAAGAGATATTCATAATATATGTATTTATCATAAGCAAGATGTGTATGCAAGGCTTTCATTTACAACAGATCCTGAAAACTCTGTTTCCACCAAAACCATCAATGGTGGTGGGAGAAATCCTGTGTTCAATGACAGTGTTCATCTCGACGTTCGGTCAATCGATACTACGCTCAAATGCGAGATATGGATGCTGAGCAGGGTCAAGAATTATCTTGAAGATCAGTTGCTTGGTTTTGCTTTGATTCCTCTAACAGAAGTTGTTGCTGTCAATGGAAAGTTAGAGAAAGAATTCGGTCTTTCGTCGACCGATTTGTTCCATTCTCCCGCCGGGTTCGTGCAGTTATCGCTTTCGTATAATGGAGCTTCTCCAGAGGTCATGGCGATTCCGACCATTGAAAGAACTGTAGGGGTGGAGGATTCAGATTTAACCAAGTCACATCCAAGTGATTTGGACATGATCGAATTCCCTGATCCGAAGATTGCGAATGAAGATCAAATAATGGTGTCTGAGTATTTCGGCATCCCCTGCTCGAATTTGGATTCTGAATCTTCTGAGAGCTTAGCCATTTCTGAGGGTGAGAATCATGGCATTTCTGTGGTCGAAAGCTTTTCAATATCGAACGACAAGTCGAGTGAAGATTCGAAGATTGATTCTCCACCTAGTAATGTGCAAAATGATGCGGTTTCTTCACCGTCGTCGCCATCAGAAGCTCGAGCGAGATCAAAATGTACAACTCAAGAACATGTTTCAGCAGCAATTACCACAAGTGAGAAAGAACAAAATGGTGAAAATTGTGAGGTTAATGATTCTTCTAGTGAGGCAGCAATTGCCAATGACACAATTAAAAAGCCATTAGTTTCTGTGAGCATTGAGCCAGAAGACAAGGTAGTGCAACAAGACATTGTGGACATGTACATGAAAAGTATGCAACAATTCACAGAGTCATTGGCAAAGATGAAGCTACCTTTGGATATTGACACTAATGGCTCTCCAAGTTCAGCTACTTCGAGCTCCGACCTGAACTTACAATCGCCCAAAAACAACGTTTCGCGTGTGTTTTACGGGAGCAGGGCGTTCTTCTGA

Coding sequence (CDS)

ATGGCTTCCCCAGTTGAGAAATTGGGTGTTTTAGAGGAAGAGATAAAGGAATGTGTTGTGGGAAATGAGAAGGAAGGAAAGGAAGTTGTTGGTGTTCTTGAGGTGTTTGTTCACCAAGCAAGAGATATTCATAATATATGTATTTATCATAAGCAAGATGTGTATGCAAGGCTTTCATTTACAACAGATCCTGAAAACTCTGTTTCCACCAAAACCATCAATGGTGGTGGGAGAAATCCTGTGTTCAATGACAGTGTTCATCTCGACGTTCGGTCAATCGATACTACGCTCAAATGCGAGATATGGATGCTGAGCAGGGTCAAGAATTATCTTGAAGATCAGTTGCTTGGTTTTGCTTTGATTCCTCTAACAGAAGTTGTTGCTGTCAATGGAAAGTTAGAGAAAGAATTCGGTCTTTCGTCGACCGATTTGTTCCATTCTCCCGCCGGGTTCGTGCAGTTATCGCTTTCGTATAATGGAGCTTCTCCAGAGGTCATGGCGATTCCGACCATTGAAAGAACTGTAGGGGTGGAGGATTCAGATTTAACCAAGTCACATCCAAGTGATTTGGACATGATCGAATTCCCTGATCCGAAGATTGCGAATGAAGATCAAATAATGGTGTCTGAGTATTTCGGCATCCCCTGCTCGAATTTGGATTCTGAATCTTCTGAGAGCTTAGCCATTTCTGAGGGTGAGAATCATGGCATTTCTGTGGTCGAAAGCTTTTCAATATCGAACGACAAGTCGAGTGAAGATTCGAAGATTGATTCTCCACCTAGTAATGTGCAAAATGATGCGGTTTCTTCACCGTCGTCGCCATCAGAAGCTCGAGCGAGATCAAAATGTACAACTCAAGAACATGTTTCAGCAGCAATTACCACAAGTGAGAAAGAACAAAATGGTGAAAATTGTGAGGTTAATGATTCTTCTAGTGAGGCAGCAATTGCCAATGACACAATTAAAAAGCCATTAGTTTCTGTGAGCATTGAGCCAGAAGACAAGGTAGTGCAACAAGACATTGTGGACATGTACATGAAAAGTATGCAACAATTCACAGAGTCATTGGCAAAGATGAAGCTACCTTTGGATATTGACACTAATGGCTCTCCAAGTTCAGCTACTTCGAGCTCCGACCTGAACTTACAATCGCCCAAAAACAACGTTTCGCGTGTGTTTTACGGGAGCAGGGCGTTCTTCTGA

Protein sequence

MASPVEKLGVLEEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVEDSDLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF
Homology
BLAST of Lsi05G005280 vs. ExPASy TrEMBL
Match: A0A0A0L7H6 (C2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G062580 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 4.2e-170
Identity = 343/404 (84.90%), Postives = 354/404 (87.62%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKEC-VVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60
           MA+PVEKLGVLEEE KEC VVGNEKE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 61  FTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120
           FTTDP NSVSTKTINGGGRNPVFND V LDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDMVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 121 LIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           LIPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASP+VM IP IE TVGVED
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPQVMVIPAIETTVGVED 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHG-I 240
           SDLTK SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL +SEGENH  I
Sbjct: 181 SDLTKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSEGENHHVI 240

Query: 241 SVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSE 300
           S VESFSIS DKSSED+K DSP S VQ  AVSSP SPSEA   SKC TQE VSA   TSE
Sbjct: 241 SSVESFSISKDKSSEDTKKDSPRSEVQKGAVSSPQSPSEAPVDSKCKTQE-VSARNGTSE 300

Query: 301 KEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLA 360
           KE+  +N E ND  S      DTI+KPLVSVSIE E+KVVQQDIV+MYMKSMQQFTESLA
Sbjct: 301 KEE--KNGEANDCCS------DTIRKPLVSVSIEAEEKVVQQDIVEMYMKSMQQFTESLA 360

Query: 361 KMKLPLDIDTN-GSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           KMKLPLDIDTN GSPS ATSSSD NLQSPKN+  RVFYGSRAFF
Sbjct: 361 KMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGPRVFYGSRAFF 395

BLAST of Lsi05G005280 vs. ExPASy TrEMBL
Match: A0A5A7VFA2 (C2 calcium-dependent membrane targeting OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001780 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 5.1e-168
Identity = 342/406 (84.24%), Postives = 355/406 (87.44%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKEC-VVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60
           MA+PVEKLGVLEEE KEC VVGNEKE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 61  FTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120
           FTTDP NSVSTKTINGGGRNPVFND+V LDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDTVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 121 LIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           LIPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASPEVM IPTIE TVGVE+
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMVIPTIETTVGVEN 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHG-I 240
           SDL K SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL +SE ENH  I
Sbjct: 181 SDLIKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSESENHHVI 240

Query: 241 SVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPS--EARARSKCTTQEHVSAAITT 300
           S VES   SNDK SED+KIDSP S +QN+AVSS  SPS  EA   SKCTTQE VSA   T
Sbjct: 241 SAVES---SNDKLSEDTKIDSPLSKMQNNAVSSSPSPSALEAPVNSKCTTQEEVSAPNGT 300

Query: 301 SEKEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTES 360
           SEKE+  +N EVND  S      DTI+KPLVSVSIE E+KVVQQDIVD YMKSMQQFTES
Sbjct: 301 SEKEE--KNGEVNDCCS------DTIRKPLVSVSIEAENKVVQQDIVDKYMKSMQQFTES 360

Query: 361 LAKMKLPLDIDTN-GSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           LAKMKLPLDIDTN GSPS ATSSSD NLQSPKN+ SRVFYGSRAFF
Sbjct: 361 LAKMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGSRVFYGSRAFF 395

BLAST of Lsi05G005280 vs. ExPASy TrEMBL
Match: A0A1S3BL87 (uncharacterized protein LOC103491056 OS=Cucumis melo OX=3656 GN=LOC103491056 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 3.3e-167
Identity = 341/406 (83.99%), Postives = 352/406 (86.70%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKEC-VVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60
           MA+PVEKLGVLEEE KEC VVGNEKE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 61  FTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120
           FTTDP NSVSTKTINGGGRNPVFND+V LDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDTVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 121 LIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           LIPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASPEVM IPTIE TVGVE+
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMVIPTIETTVGVEN 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHG-I 240
           SDL K SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL +SE ENH  I
Sbjct: 181 SDLIKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSESENHHVI 240

Query: 241 SVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPS--EARARSKCTTQEHVSAAITT 300
           S VES       SSED+KIDSP S VQNDAVSS  SPS  EA   SKCTTQE VSA   T
Sbjct: 241 SAVES------SSSEDTKIDSPLSKVQNDAVSSSPSPSALEAPVNSKCTTQEEVSAPNGT 300

Query: 301 SEKEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTES 360
           SEKE+  +N EVND  S      DTI+KPLVSVSIE E+KVVQQDIVD YMKSMQQFTES
Sbjct: 301 SEKEE--KNGEVNDCCS------DTIRKPLVSVSIEAENKVVQQDIVDKYMKSMQQFTES 360

Query: 361 LAKMKLPLDIDTN-GSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           LAKMKLPLDIDTN GSPS ATSSSD NLQSPKN+ SRVFYGSRAFF
Sbjct: 361 LAKMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGSRVFYGSRAFF 392

BLAST of Lsi05G005280 vs. ExPASy TrEMBL
Match: A0A6J1DSI6 (uncharacterized protein LOC111023111 OS=Momordica charantia OX=3673 GN=LOC111023111 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.4e-157
Identity = 318/413 (77.00%), Postives = 347/413 (84.02%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 60
           MASPV  LGV E E +ECVV N KEG+ VVGVLEV VHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASPVNNLGVSEHEKQECVVSNGKEGEGVVGVLEVLVHQARDIHNICIYHKQDVYARLSF 60

Query: 61  TTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFAL 120
           T+DP NSVSTKTINGGGRNPVFN++V L VRS DT+LKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TSDPSNSVSTKTINGGGRNPVFNENVRLSVRSNDTSLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 121 IPLTEVV-AVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           IPL EVV AVNGKLEKEF LSS+DLFHSPAGFVQLSLSYNGASPEVM IP +E    V+D
Sbjct: 121 IPLAEVVAAVNGKLEKEFSLSSSDLFHSPAGFVQLSLSYNGASPEVMEIPALETIATVQD 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENH--- 240
           SD+ K SHPSDLDMIEFPDPKI NEDQ+MVSEY GIPCS+LDSE SESLAIS+GENH   
Sbjct: 181 SDVNKLSHPSDLDMIEFPDPKIVNEDQMMVSEYIGIPCSDLDSERSESLAISDGENHHLS 240

Query: 241 ----GISVVESFSISNDKSSEDSKIDSPPSNVQNDAVSS---PSSPSEARARSKCTTQ-E 300
               GI VVE FS+ N+KS++ SKIDSPPS+V  DAVSS    S  SEA A SK TTQ E
Sbjct: 241 PGIGGIPVVEKFSMPNEKSNQASKIDSPPSSVPTDAVSSLPASSESSEAAASSKATTQDE 300

Query: 301 HVSAAITTSEKEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMK 360
           +VSAA  +SEKEQN EN E+NDS SEA  A +TI  P+VSV+IEP+DKVVQQDIVDMYMK
Sbjct: 301 YVSAANASSEKEQNTENGEINDSCSEA--AGETITNPIVSVNIEPDDKVVQQDIVDMYMK 360

Query: 361 SMQQFTESLAKMKLPLDIDTNGSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           SMQQFTESLAKMKLPLDID +G PSS +S+SD NLQSPKN+  RVFYGSRAFF
Sbjct: 361 SMQQFTESLAKMKLPLDID-SGPPSSGSSTSDPNLQSPKNSGPRVFYGSRAFF 410

BLAST of Lsi05G005280 vs. ExPASy TrEMBL
Match: A0A6J1HKQ8 (uncharacterized protein LOC111465051 OS=Cucurbita maxima OX=3661 GN=LOC111465051 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 4.0e-157
Identity = 316/402 (78.61%), Postives = 336/402 (83.58%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 60
           MASPVE LGV+EEE KEC           +GVLEVFVHQARDIHNICIYHKQDVYA+LSF
Sbjct: 1   MASPVENLGVVEEEKKEC-----------IGVLEVFVHQARDIHNICIYHKQDVYAKLSF 60

Query: 61  TTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFAL 120
           TT PENSVSTKTINGGG+NPVFN+ V LDV  IDTTLKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TTHPENSVSTKTINGGGKNPVFNERVRLDVPMIDTTLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 121 IPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVEDS 180
           IPLTEVV VNGKLEKEF LSSTDLFHSPAGFV LSLSYNGA PEVMAIP +E  V VEDS
Sbjct: 121 IPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVLLSLSYNGAFPEVMAIPVVETIVEVEDS 180

Query: 181 DLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVV 240
           D+T+ HPSDLDMIEFP+PKIANEDQIMVSEYFGIPCSNLDSESSESL ISE ENHGI VV
Sbjct: 181 DVTEPHPSDLDMIEFPNPKIANEDQIMVSEYFGIPCSNLDSESSESLTISEVENHGIPVV 240

Query: 241 ESFSISNDKSS-EDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKE 300
           ESF + NDKSS EDSKIDSPP+NVQND VSSPS  S+A+A S             T++KE
Sbjct: 241 ESFPMLNDKSSEEDSKIDSPPNNVQNDTVSSPSL-SKAQASSN-----------GTADKE 300

Query: 301 QNGENCEVNDSSSEAAIANDTIKKPLVSVSIE-PEDKVVQQDIVDMYMKSMQQFTESLAK 360
           +  E+ EVN SSS      D I KPLVSVS+E PEDKVVQQDIVDMYMKSMQQFTESLAK
Sbjct: 301 ETAESGEVNGSSS------DKITKPLVSVSVEPPEDKVVQQDIVDMYMKSMQQFTESLAK 360

Query: 361 MKLPLDIDTNGSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           MKLPLDIDTNGSPSS TSSSD NL+SPKN+ SRVFYGSRAFF
Sbjct: 361 MKLPLDIDTNGSPSSTTSSSDPNLKSPKNSGSRVFYGSRAFF 373

BLAST of Lsi05G005280 vs. NCBI nr
Match: XP_038902970.1 (uncharacterized protein LOC120089682 [Benincasa hispida] >XP_038902971.1 uncharacterized protein LOC120089682 [Benincasa hispida])

HSP 1 Score: 673.7 bits (1737), Expect = 9.8e-190
Identity = 364/401 (90.77%), Postives = 374/401 (93.27%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 60
           MASPVEKLGVLEEE KECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASPVEKLGVLEEEKKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 60

Query: 61  TTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFAL 120
           TTDP NSVSTKTINGGGRNPVFN+SV LDVRSIDT LKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TTDPANSVSTKTINGGGRNPVFNESVRLDVRSIDTMLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 121 IPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVEDS 180
           IPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASPEVMAI TIE TVGVEDS
Sbjct: 121 IPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIRTIETTVGVEDS 180

Query: 181 DLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVV 240
           DLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL ISEGEN GI VV
Sbjct: 181 DLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPISEGENQGILVV 240

Query: 241 ESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPSEARARSKC-TTQEHVSAAITTSEKE 300
           ++FS+SNDKSSE+SKIDSPPSNVQNDAVSSP+SPSEA A SKC TTQEHVSA   TSEKE
Sbjct: 241 KNFSMSNDKSSEESKIDSPPSNVQNDAVSSPTSPSEAPASSKCTTTQEHVSAPNETSEKE 300

Query: 301 QNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKM 360
           QNGEN EVNDSS+EA     TI KPLVSVSIE EDKVVQQDIVDMYMKSMQQFTESLAKM
Sbjct: 301 QNGENGEVNDSSTEA-----TITKPLVSVSIEAEDKVVQQDIVDMYMKSMQQFTESLAKM 360

Query: 361 KLPLDIDTNGSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           KLPLDIDTNGSP+SATSSSD NLQSPKN+ SRVFYGSRAFF
Sbjct: 361 KLPLDIDTNGSPTSATSSSDPNLQSPKNSGSRVFYGSRAFF 396

BLAST of Lsi05G005280 vs. NCBI nr
Match: XP_004149578.1 (uncharacterized protein LOC101220782 [Cucumis sativus] >XP_004149579.1 uncharacterized protein LOC101220782 [Cucumis sativus] >KGN56056.1 hypothetical protein Csa_010562 [Cucumis sativus])

HSP 1 Score: 607.4 bits (1565), Expect = 8.6e-170
Identity = 343/404 (84.90%), Postives = 354/404 (87.62%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKEC-VVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60
           MA+PVEKLGVLEEE KEC VVGNEKE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 61  FTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120
           FTTDP NSVSTKTINGGGRNPVFND V LDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDMVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 121 LIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           LIPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASP+VM IP IE TVGVED
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPQVMVIPAIETTVGVED 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHG-I 240
           SDLTK SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL +SEGENH  I
Sbjct: 181 SDLTKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSEGENHHVI 240

Query: 241 SVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSE 300
           S VESFSIS DKSSED+K DSP S VQ  AVSSP SPSEA   SKC TQE VSA   TSE
Sbjct: 241 SSVESFSISKDKSSEDTKKDSPRSEVQKGAVSSPQSPSEAPVDSKCKTQE-VSARNGTSE 300

Query: 301 KEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLA 360
           KE+  +N E ND  S      DTI+KPLVSVSIE E+KVVQQDIV+MYMKSMQQFTESLA
Sbjct: 301 KEE--KNGEANDCCS------DTIRKPLVSVSIEAEEKVVQQDIVEMYMKSMQQFTESLA 360

Query: 361 KMKLPLDIDTN-GSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           KMKLPLDIDTN GSPS ATSSSD NLQSPKN+  RVFYGSRAFF
Sbjct: 361 KMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGPRVFYGSRAFF 395

BLAST of Lsi05G005280 vs. NCBI nr
Match: KAA0065787.1 (C2 calcium-dependent membrane targeting [Cucumis melo var. makuwa] >TYJ96526.1 C2 calcium-dependent membrane targeting [Cucumis melo var. makuwa])

HSP 1 Score: 600.5 bits (1547), Expect = 1.1e-167
Identity = 342/406 (84.24%), Postives = 355/406 (87.44%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKEC-VVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60
           MA+PVEKLGVLEEE KEC VVGNEKE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 61  FTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120
           FTTDP NSVSTKTINGGGRNPVFND+V LDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDTVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 121 LIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           LIPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASPEVM IPTIE TVGVE+
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMVIPTIETTVGVEN 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHG-I 240
           SDL K SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL +SE ENH  I
Sbjct: 181 SDLIKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSESENHHVI 240

Query: 241 SVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPS--EARARSKCTTQEHVSAAITT 300
           S VES   SNDK SED+KIDSP S +QN+AVSS  SPS  EA   SKCTTQE VSA   T
Sbjct: 241 SAVES---SNDKLSEDTKIDSPLSKMQNNAVSSSPSPSALEAPVNSKCTTQEEVSAPNGT 300

Query: 301 SEKEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTES 360
           SEKE+  +N EVND  S      DTI+KPLVSVSIE E+KVVQQDIVD YMKSMQQFTES
Sbjct: 301 SEKEE--KNGEVNDCCS------DTIRKPLVSVSIEAENKVVQQDIVDKYMKSMQQFTES 360

Query: 361 LAKMKLPLDIDTN-GSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           LAKMKLPLDIDTN GSPS ATSSSD NLQSPKN+ SRVFYGSRAFF
Sbjct: 361 LAKMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGSRVFYGSRAFF 395

BLAST of Lsi05G005280 vs. NCBI nr
Match: XP_008449084.1 (PREDICTED: uncharacterized protein LOC103491056 [Cucumis melo] >XP_008449085.1 PREDICTED: uncharacterized protein LOC103491056 [Cucumis melo])

HSP 1 Score: 597.8 bits (1540), Expect = 6.8e-167
Identity = 341/406 (83.99%), Postives = 352/406 (86.70%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKEC-VVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60
           MA+PVEKLGVLEEE KEC VVGNEKE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 61  FTTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120
           FTTDP NSVSTKTINGGGRNPVFND+V LDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDTVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 121 LIPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVED 180
           LIPLTEVVAV+ KLEKEF LSSTDLFHSPAGFVQLSLSYNGASPEVM IPTIE TVGVE+
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMVIPTIETTVGVEN 180

Query: 181 SDLTK-SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHG-I 240
           SDL K SHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL +SE ENH  I
Sbjct: 181 SDLIKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSESENHHVI 240

Query: 241 SVVESFSISNDKSSEDSKIDSPPSNVQNDAVSSPSSPS--EARARSKCTTQEHVSAAITT 300
           S VES       SSED+KIDSP S VQNDAVSS  SPS  EA   SKCTTQE VSA   T
Sbjct: 241 SAVES------SSSEDTKIDSPLSKVQNDAVSSSPSPSALEAPVNSKCTTQEEVSAPNGT 300

Query: 301 SEKEQNGENCEVNDSSSEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTES 360
           SEKE+  +N EVND  S      DTI+KPLVSVSIE E+KVVQQDIVD YMKSMQQFTES
Sbjct: 301 SEKEE--KNGEVNDCCS------DTIRKPLVSVSIEAENKVVQQDIVDKYMKSMQQFTES 360

Query: 361 LAKMKLPLDIDTN-GSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           LAKMKLPLDIDTN GSPS ATSSSD NLQSPKN+ SRVFYGSRAFF
Sbjct: 361 LAKMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGSRVFYGSRAFF 392

BLAST of Lsi05G005280 vs. NCBI nr
Match: KAG6577499.1 (hypothetical protein SDJN03_25073, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015556.1 hypothetical protein SDJN02_23192, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.1 bits (1468), Expect = 1.5e-158
Identity = 320/402 (79.60%), Postives = 336/402 (83.58%), Query Frame = 0

Query: 1   MASPVEKLGVLEEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 60
           MASPVE L V+EEE KEC           +GVLEVFVHQARDIHNICIYHKQDVYA+LSF
Sbjct: 1   MASPVENLSVVEEEKKEC-----------IGVLEVFVHQARDIHNICIYHKQDVYAKLSF 60

Query: 61  TTDPENSVSTKTINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFAL 120
           TT PENSVSTKTINGGG+NPVFN+ V LDV  IDTTLKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TTHPENSVSTKTINGGGKNPVFNERVRLDVPMIDTTLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 121 IPLTEVVAVNGKLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVEDS 180
           IPLTEVV VNGKLEKEF LSSTDLFHSPAGFVQLSLSYNGASPEVMAIP +E  V VEDS
Sbjct: 121 IPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVVETIVEVEDS 180

Query: 181 DLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVV 240
           DLT+  PSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESL ISE ENHGI VV
Sbjct: 181 DLTEPRPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLTISEVENHGIPVV 240

Query: 241 ESFSISNDKSS-EDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKE 300
           ESF   NDKSS EDSK+DSPP+NVQND VSSPSS S+A+A S             T++KE
Sbjct: 241 ESFPTMNDKSSEEDSKVDSPPNNVQNDTVSSPSS-SKAQASSN-----------GTADKE 300

Query: 301 QNGENCEVNDSSSEAAIANDTIKKPLVSVSIE-PEDKVVQQDIVDMYMKSMQQFTESLAK 360
           Q  EN EV  SSS      DTI KPLVSVS+E PEDKVVQQDIVDMYMKSMQQFTESLAK
Sbjct: 301 QTAENGEVYGSSS------DTITKPLVSVSVEPPEDKVVQQDIVDMYMKSMQQFTESLAK 360

Query: 361 MKLPLDIDTNGSPSSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           MKLPLDIDTN SPSSATSSSD NL+SPKN+ SRVFYGSRAFF
Sbjct: 361 MKLPLDIDTNSSPSSATSSSDPNLKSPKNSGSRVFYGSRAFF 373

BLAST of Lsi05G005280 vs. TAIR 10
Match: AT5G55530.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.9e-107
Identity = 216/379 (56.99%), Postives = 272/379 (71.77%), Query Frame = 0

Query: 27  KEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPENSVSTKTINGGGRNPVFNDSV 86
           +++VG LEV+VHQARDIHNICIYHKQDVYA+L  T+DP+ SVSTK INGGGRNPVF+D+V
Sbjct: 47  QDLVGALEVYVHQARDIHNICIYHKQDVYAKLCLTSDPDKSVSTKIINGGGRNPVFDDNV 106

Query: 87  HLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVAVNGKLEKEFGLSSTDLFH 146
            LDVR +DT+LKCEI+M+SRVKNYLEDQLLGF L+P++E++  NGKLEKEF LSSTDL+H
Sbjct: 107 KLDVRVLDTSLKCEIYMMSRVKNYLEDQLLGFTLVPMSELLFKNGKLEKEFSLSSTDLYH 166

Query: 147 SPAGFVQLSLSYNGASPEVMAIPTIERTVGVE----DSDLTKSHPSDLDMIEFPDPKIAN 206
           SPAGFVQLSLSY G+ P+VMAIP++  +V ++    D + ++S P +LD IEFPDP +AN
Sbjct: 167 SPAGFVQLSLSYYGSYPDVMAIPSMPSSVSIDETTKDPEGSESVPGELDKIEFPDPNVAN 226

Query: 207 EDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVVESFSISNDKSSEDSKIDSPPSN 266
           E++ MVSEYFGI CS +DSE+S+SL  S+ ENH  + V S            K DSP S+
Sbjct: 227 ENEKMVSEYFGISCSTIDSETSDSLVTSDAENHVTNSVTSI----------LKQDSPESS 286

Query: 267 VQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKEQNGENCEVNDSSSEAAIANDTIK 326
              +  +SP     A A S   T  H   ++  S+        E +  +SE     +   
Sbjct: 287 NATNGAASP----HASAHSATETPNHEHLSVVNSKASSQESESEASGETSE-----EKTV 346

Query: 327 KPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGSPSSATSSSDLNL 386
           K +++V +EPE KVVQQDIVDMYMKSMQQFT+SLAKMKLPLDID + + S  +SS    L
Sbjct: 347 KSVLTVKVEPESKVVQQDIVDMYMKSMQQFTDSLAKMKLPLDID-SPTKSENSSSDSQKL 405

Query: 387 QSPK-NNVSRVFYGSRAFF 401
            +PK NN SRVFYGSR FF
Sbjct: 407 PTPKSNNGSRVFYGSRPFF 405

BLAST of Lsi05G005280 vs. TAIR 10
Match: AT5G55530.3 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.9e-107
Identity = 216/379 (56.99%), Postives = 272/379 (71.77%), Query Frame = 0

Query: 27  KEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPENSVSTKTINGGGRNPVFNDSV 86
           +++VG LEV+VHQARDIHNICIYHKQDVYA+L  T+DP+ SVSTK INGGGRNPVF+D+V
Sbjct: 47  QDLVGALEVYVHQARDIHNICIYHKQDVYAKLCLTSDPDKSVSTKIINGGGRNPVFDDNV 106

Query: 87  HLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVAVNGKLEKEFGLSSTDLFH 146
            LDVR +DT+LKCEI+M+SRVKNYLEDQLLGF L+P++E++  NGKLEKEF LSSTDL+H
Sbjct: 107 KLDVRVLDTSLKCEIYMMSRVKNYLEDQLLGFTLVPMSELLFKNGKLEKEFSLSSTDLYH 166

Query: 147 SPAGFVQLSLSYNGASPEVMAIPTIERTVGVE----DSDLTKSHPSDLDMIEFPDPKIAN 206
           SPAGFVQLSLSY G+ P+VMAIP++  +V ++    D + ++S P +LD IEFPDP +AN
Sbjct: 167 SPAGFVQLSLSYYGSYPDVMAIPSMPSSVSIDETTKDPEGSESVPGELDKIEFPDPNVAN 226

Query: 207 EDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVVESFSISNDKSSEDSKIDSPPSN 266
           E++ MVSEYFGI CS +DSE+S+SL  S+ ENH  + V S            K DSP S+
Sbjct: 227 ENEKMVSEYFGISCSTIDSETSDSLVTSDAENHVTNSVTSI----------LKQDSPESS 286

Query: 267 VQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKEQNGENCEVNDSSSEAAIANDTIK 326
              +  +SP     A A S   T  H   ++  S+        E +  +SE     +   
Sbjct: 287 NATNGAASP----HASAHSATETPNHEHLSVVNSKASSQESESEASGETSE-----EKTV 346

Query: 327 KPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGSPSSATSSSDLNL 386
           K +++V +EPE KVVQQDIVDMYMKSMQQFT+SLAKMKLPLDID + + S  +SS    L
Sbjct: 347 KSVLTVKVEPESKVVQQDIVDMYMKSMQQFTDSLAKMKLPLDID-SPTKSENSSSDSQKL 405

Query: 387 QSPK-NNVSRVFYGSRAFF 401
            +PK NN SRVFYGSR FF
Sbjct: 407 PTPKSNNGSRVFYGSRPFF 405

BLAST of Lsi05G005280 vs. TAIR 10
Match: AT5G55530.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.9e-107
Identity = 216/379 (56.99%), Postives = 272/379 (71.77%), Query Frame = 0

Query: 27  KEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPENSVSTKTINGGGRNPVFNDSV 86
           +++VG LEV+VHQARDIHNICIYHKQDVYA+L  T+DP+ SVSTK INGGGRNPVF+D+V
Sbjct: 47  QDLVGALEVYVHQARDIHNICIYHKQDVYAKLCLTSDPDKSVSTKIINGGGRNPVFDDNV 106

Query: 87  HLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVAVNGKLEKEFGLSSTDLFH 146
            LDVR +DT+LKCEI+M+SRVKNYLEDQLLGF L+P++E++  NGKLEKEF LSSTDL+H
Sbjct: 107 KLDVRVLDTSLKCEIYMMSRVKNYLEDQLLGFTLVPMSELLFKNGKLEKEFSLSSTDLYH 166

Query: 147 SPAGFVQLSLSYNGASPEVMAIPTIERTVGVE----DSDLTKSHPSDLDMIEFPDPKIAN 206
           SPAGFVQLSLSY G+ P+VMAIP++  +V ++    D + ++S P +LD IEFPDP +AN
Sbjct: 167 SPAGFVQLSLSYYGSYPDVMAIPSMPSSVSIDETTKDPEGSESVPGELDKIEFPDPNVAN 226

Query: 207 EDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVVESFSISNDKSSEDSKIDSPPSN 266
           E++ MVSEYFGI CS +DSE+S+SL  S+ ENH  + V S            K DSP S+
Sbjct: 227 ENEKMVSEYFGISCSTIDSETSDSLVTSDAENHVTNSVTSI----------LKQDSPESS 286

Query: 267 VQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKEQNGENCEVNDSSSEAAIANDTIK 326
              +  +SP     A A S   T  H   ++  S+        E +  +SE     +   
Sbjct: 287 NATNGAASP----HASAHSATETPNHEHLSVVNSKASSQESESEASGETSE-----EKTV 346

Query: 327 KPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGSPSSATSSSDLNL 386
           K +++V +EPE KVVQQDIVDMYMKSMQQFT+SLAKMKLPLDID + + S  +SS    L
Sbjct: 347 KSVLTVKVEPESKVVQQDIVDMYMKSMQQFTDSLAKMKLPLDID-SPTKSENSSSDSQKL 405

Query: 387 QSPK-NNVSRVFYGSRAFF 401
            +PK NN SRVFYGSR FF
Sbjct: 407 PTPKSNNGSRVFYGSRPFF 405

BLAST of Lsi05G005280 vs. TAIR 10
Match: AT1G50570.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-93
Identity = 199/389 (51.16%), Postives = 266/389 (68.38%), Query Frame = 0

Query: 12  EEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPENSVSTK 71
           E + K  V+ ++ +    +GVLEVFVHQARDIHNICIYHKQDVYA+L  T DPENS+STK
Sbjct: 22  ETKTKNIVMSSDSD--SFIGVLEVFVHQARDIHNICIYHKQDVYAKLCLTNDPENSLSTK 81

Query: 72  TINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVAVNG 131
            INGGG+NPVF+D++  DV+++D +LKCEI+M+SRVKNYLEDQLLGF+L+PL+EV+  NG
Sbjct: 82  IINGGGQNPVFDDTLQFDVKNLDCSLKCEIFMMSRVKNYLEDQLLGFSLVPLSEVIVRNG 141

Query: 132 KLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVEDSDLTKSHPSDLD 191
           KLEKEF LSSTDL+HSPAGFV+LSLSY G SP+VM IP       V  +D T+  P + D
Sbjct: 142 KLEKEFSLSSTDLYHSPAGFVELSLSYAGDSPDVMHIP------AVPTADETELAPIEFD 201

Query: 192 MIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVVESFSISNDKSS 251
             EF DPKI  E+  MVS+YF   CS+ D  +S      E  +   +VVE+   + D++ 
Sbjct: 202 ESEFLDPKIVCENNQMVSKYFSTTCSDSDDFASSETGFVEVNSILSAVVET---AVDEA- 261

Query: 252 EDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKEQNGENCEVNDSS 311
                 +P ++V  + +SSPS+   + +       +  S    +  +++  +  ++  S 
Sbjct: 262 ------APANSVSTNGISSPSTAVSSGSSGTHDVSKQSSEGNNSDSEQEAKKPTDIIKSG 321

Query: 312 SEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGSP 371
                  + + KP+++V+IEPE KVVQQDIVDMY KS+QQFTESLAKMKLPLDID   SP
Sbjct: 322 DLDKTDEEAVVKPVLTVNIEPEQKVVQQDIVDMYTKSLQQFTESLAKMKLPLDID---SP 381

Query: 372 SSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           + + +SS  + Q+PK+  SRVFYGSRAFF
Sbjct: 382 TQSENSSS-SQQTPKSASSRVFYGSRAFF 388

BLAST of Lsi05G005280 vs. TAIR 10
Match: AT1G50570.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-93
Identity = 199/389 (51.16%), Postives = 266/389 (68.38%), Query Frame = 0

Query: 12  EEEIKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPENSVSTK 71
           E + K  V+ ++ +    +GVLEVFVHQARDIHNICIYHKQDVYA+L  T DPENS+STK
Sbjct: 22  ETKTKNIVMSSDSD--SFIGVLEVFVHQARDIHNICIYHKQDVYAKLCLTNDPENSLSTK 81

Query: 72  TINGGGRNPVFNDSVHLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVAVNG 131
            INGGG+NPVF+D++  DV+++D +LKCEI+M+SRVKNYLEDQLLGF+L+PL+EV+  NG
Sbjct: 82  IINGGGQNPVFDDTLQFDVKNLDCSLKCEIFMMSRVKNYLEDQLLGFSLVPLSEVIVRNG 141

Query: 132 KLEKEFGLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPTIERTVGVEDSDLTKSHPSDLD 191
           KLEKEF LSSTDL+HSPAGFV+LSLSY G SP+VM IP       V  +D T+  P + D
Sbjct: 142 KLEKEFSLSSTDLYHSPAGFVELSLSYAGDSPDVMHIP------AVPTADETELAPIEFD 201

Query: 192 MIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLAISEGENHGISVVESFSISNDKSS 251
             EF DPKI  E+  MVS+YF   CS+ D  +S      E  +   +VVE+   + D++ 
Sbjct: 202 ESEFLDPKIVCENNQMVSKYFSTTCSDSDDFASSETGFVEVNSILSAVVET---AVDEA- 261

Query: 252 EDSKIDSPPSNVQNDAVSSPSSPSEARARSKCTTQEHVSAAITTSEKEQNGENCEVNDSS 311
                 +P ++V  + +SSPS+   + +       +  S    +  +++  +  ++  S 
Sbjct: 262 ------APANSVSTNGISSPSTAVSSGSSGTHDVSKQSSEGNNSDSEQEAKKPTDIIKSG 321

Query: 312 SEAAIANDTIKKPLVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGSP 371
                  + + KP+++V+IEPE KVVQQDIVDMY KS+QQFTESLAKMKLPLDID   SP
Sbjct: 322 DLDKTDEEAVVKPVLTVNIEPEQKVVQQDIVDMYTKSLQQFTESLAKMKLPLDID---SP 381

Query: 372 SSATSSSDLNLQSPKNNVSRVFYGSRAFF 401
           + + +SS  + Q+PK+  SRVFYGSRAFF
Sbjct: 382 TQSENSSS-SQQTPKSASSRVFYGSRAFF 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L7H64.2e-17084.90C2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G062580 PE=4 SV=... [more]
A0A5A7VFA25.1e-16884.24C2 calcium-dependent membrane targeting OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BL873.3e-16783.99uncharacterized protein LOC103491056 OS=Cucumis melo OX=3656 GN=LOC103491056 PE=... [more]
A0A6J1DSI61.4e-15777.00uncharacterized protein LOC111023111 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1HKQ84.0e-15778.61uncharacterized protein LOC111465051 OS=Cucurbita maxima OX=3661 GN=LOC111465051... [more]
Match NameE-valueIdentityDescription
XP_038902970.19.8e-19090.77uncharacterized protein LOC120089682 [Benincasa hispida] >XP_038902971.1 unchara... [more]
XP_004149578.18.6e-17084.90uncharacterized protein LOC101220782 [Cucumis sativus] >XP_004149579.1 uncharact... [more]
KAA0065787.11.1e-16784.24C2 calcium-dependent membrane targeting [Cucumis melo var. makuwa] >TYJ96526.1 C... [more]
XP_008449084.16.8e-16783.99PREDICTED: uncharacterized protein LOC103491056 [Cucumis melo] >XP_008449085.1 P... [more]
KAG6577499.11.5e-15879.60hypothetical protein SDJN03_25073, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT5G55530.14.9e-10756.99Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G55530.34.9e-10756.99Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G55530.24.9e-10756.99Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G50570.12.4e-9351.16Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G50570.22.4e-9351.16Calcium-dependent lipid-binding (CaLB domain) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainSMARTSM00239C2_3ccoord: 32..135
e-value: 4.9E-6
score: 36.1
IPR000008C2 domainPFAMPF00168C2coord: 31..127
e-value: 2.1E-8
score: 34.4
IPR000008C2 domainPROSITEPS50004C2coord: 11..137
score: 12.850475
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 27..168
e-value: 2.9E-15
score: 58.6
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 29..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..311
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..311
NoneNo IPR availablePANTHERPTHR31208EXPRESSED PROTEINcoord: 21..400
NoneNo IPR availablePANTHERPTHR31208:SF2DOMAIN-CONTAINING PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 21..400
NoneNo IPR availableCDDcd00030C2coord: 33..139
e-value: 2.81633E-11
score: 57.8471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G005280.1Lsi05G005280.1mRNA