Lsi05G004500 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G004500
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr05: 5759246 .. 5764646 (-)
RNA-Seq Expression	Lsi05G004500
Synteny	Lsi05G004500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCCGATCCATCGCCAGGTTCCTTCTAATTAACTCTCAATTAATTAATTCCTGATTCTTTTTTTTTTGTCCTTTGATCGTGACCGTTGATTTTCCTTTTCTTTTTTTTTTTTTAGAGAGATTGAGATTATCGGTAATGGTCTTCTCAGTGGCCGTTTCAGGTCGTTTTCTTCGGTTTCCAGTCGGAGAAGGTGAGGATTCCATTATTTCTTTTCTTCTTCGATTTTGATCTTTTCGTTTTGGCTGTCTTGATTTCTCTTAAGAGAAAAAAATAAAAATTAATGGAGGTTTTTCCATGGGAAATTTTTAAAAAAATAAAATAAAAAAAAACTATTTATAGAAAAAAAGTTTGAATTTATTAGTAGTAAAAATTATAGAAGTCTATTAATAATAGATGCTAATAGAAGTATATCAATATCTATCCTTTTTTTTTTACTATTTTCTGTAAATACTTTGACATTTTTTTCCATGCGTGAAAATTTATCTTTATTTTATATATAATTATAAATATAATGATTTTTTTTTCTTTTCTTCATTTCGCGAAACTAAAAACTAGTGTCTCCTTAAGTAAGCAATAAGTTTTTAAAAATTAAATTTGTATATAGGTATTACGATTTGTTTGTTTTGTTAGACGTGTATTAAAAGAAATGAAGCTAGATTTTAAAAATAAAATAAAAGAAATTACTATAAACAGAAAAAAATCAAACTATTTAGAAATATAGAAAAATTTCACTGTAATAAATCGTAATAGATTTCTATCGCTTTCTATCGCTCAAGCAAATAAATTCTATCGCTTGAATGATAGAAAGCGATAGAATTCTAAATGGTCTATCACTGATAGACTGGAAAATTTTTCTATATTTATAAATAATTTGGCTCATTTTTCTATATTTGAAAAGAGTCCATAAAATAATTAGTTTTATTTTTTTATTTAAAATTGACTAAAAATTTGAATGTTTATATAAGAAAGATGAAAAATCATGTAAGAAAATGGTGAGAAAACAAATACAATTTTTAAAAATCAAATAGTTATAATGATTTCTTATATTTTGAGTTTCTCAAATTTTAAAAGTTAAATTATATAAAATAATCCTAAATTTTGGGATTTACTTTCTGGATTTGAAACATTTAATTTTTACTCTTAAATTTTAAAATTTGTTCCAAATATATTCTTTTAAGTTTTGAAATTGTTCCAAAAATATATTTTTTTTGTTAAAATAAATTATCAATTACGGATGTGGCAACTCATGTGTAGAAAATGGAAGACCCAAATGTGTGAGTCGATTGTCCATATATATGTGGGACAAAGTCTTTAGAGTTATTCTAAATTTTGTCCTATTTTTAAATTCCAAGACTATTTACTTATATTAAAAATTAATAGGTTAAAATATTATTTTAATTCTTATATTTTGAATTTTTGTTAATTTTAATTTTTTTATACTTTTAAATTTTAAAATTTAGTCCATATATTTTTAATAAATATTAAATTTAGTCCCTCAAAATTAATGTTCATTGATATTGGTTAAATAATAATAATAATAATAATTTTCTTGAAAAAATATATTAGGTGAATATTTTTTAAAATTTATCTTGAAAAGGTTAATAAATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAATCAAGCAATAGGATTAAAGTATAAGACTAAAATTGTAATTTAACCAAATTAATAGGAGTGGGAATAAAAACCATTTATAAAGTGATGAGCATGGCCCATGGGAGGATGATCTTTTATTTATGCAAAAAGAAAGTCACTTAGAATAATATAACTTTTTTCCTTTATAAAAAATATATTATCCAATTGTTTTTTTTGAAAGAATTATTATTCTTTAAAAAATGTATATATTAAGTAATATCCAAATAATAATTGAGCTACAAGTTTTAATGACTTGACTAAGATTATTTTTTCTTCTTCTCAAAAGTAGATAAATGAGAAAAATGTTTCATAAACATTATGGCTTACTTGGAGAACATTTTGAATTAATTTTTCTTCTTTTGTCTATAGAAGTAATTATTTTTAAAAATTATAGTCACATGTTGCATATTAAAGTTTGTTGTGCAATTTAAACAACTATATATTTTCAAGTACATAATTTTTATACGAAAATCATCAACCAGTCATTTATTAAAAATAAACATATAGGGAAGTTGGCTATATTATTTGTCATGCATATGAAAATGAAATAAAACTAAAGGGTAATTATAATGATTAGCAATTTTAAGGATAATAATCAAGTGCATAGCAACATTTCAAAAAAATTGTAAATATAGCAAAATCTATTAGTGATAGACCACCTAGACCATAAAAGTCTATTAGCGGTAAAAGTCTATCACTGACAGACTTTGTTATATTTACAATTTTTAAAAAATGTTGCTATATATGCTAATACTTTGAATTTGATTGCTATATTTGCAACTGTATAAAAAAAAAGACATATTATGTTTGTTGTTAATGTGCTAAATCTCGAAACATTTCATCAATAAATTAGTATTTTTATTATATCGTATAAAAATTTATGAAATATGAAAAATAAGTAATATTTGTTACTAATATAAAATAATAAATATGTTAACTTGTCTAAAATGTGAAATATTTATTTATTAATTTAAATAATTTTTTGGATTTTTTAAATTTTATAATGCTTTTAGAAATATTCATTGACACGACATTTACTTGATGTTTTCATTGACATTTTAGTAAAATCATGACATTTCAAGTCTTGGTTGTTACAAATTATTTGAATTGTAAGTCAAATTTCAACTAAAGAAGTAAAAAGAAAAAAGTGTTTGCATAAACGTAGTTCAACTGACATAAAATATGAACTCTCGATCTCAAGGTTGGTTTAGATTATTATTATTATTATTATTTAACTTGACTAAAAGTACATAACGTATCATAATTTTTAAAATACATCAAAAGATAAAAAGAAAAAGCTAATTTCTAAAAACTGAAAATTGAAAATAAAATAGTTGTTAAAGTAGCCTAAATCAAACAATATAAATAGAAATCAGCGTAAATGTTCGTGTGGATTGACTTTTGAAGTGTTTATATACATTTTTTTTATTTATAAATACTTATTTAAACACTTAAAAAATCAATTCAAACAGAACCTGAATTTAAACTCAATTCTTTATCCAAGTTTTGAGGTTCTAACATTCTAAATAACTTGTATATTATCACGTGCAACAATTTTGATCGTGGATTTGAGTCTTTGTAATTGTACATTATTTGTATTTGAAGATTCACTTTAGATTTCAATTTTTTTCTTTTGTACACAATGAAAAAAATCAAACATTTGACGTGAAAGTTGATAATACATTCTTAATTGTTATTATTATTATTATATTATTTAAATCATAATGAATTATTACTAAAAAGAAATGTGGTAATGAATATAGGAATAGGACTCATTTTCATGGTGTCATTTTCGGGCTTATAGCTAAATATTTATTACATTAAGCGGAAAAAGAATTGTGACATAAAAGACAGCTGCAGAAAGCGAGAGGTGTGAGGCCACTCATGGGATCCTTTTTGCTCAATTTATTAGATTAATGGTATATATATATATATATAACATTTTCATTCCTATCTTTCCAAGATTTGTTAAATTTTAGTTTTCTTACTCTTAATTATCTAATTTTAATTTGTATATTTTTAATAAATCTTAAATTTAATATCTTCAAGTTAATTTTTATCGAAATTAGTTATAATAAAGAGAAATTTTTAAAAATGAAAAAATACGAGAAACTATTTACACAAAATAACAAAATTTTTAAATAGTTGTGATAGATACAAATAGAAGTCTATCAGGGTCTATCAATTATAGAAATGATAGAAATTTATCATTAATAAATGCTGATAGAAGTCTATCAATGTCTATTAGTATTTTTTTTCCTATTTTCTGTAAATAGTTTGACATTTTTTCTATCAGTGAAAATTTTCCAAAAAAAATCGTTATTATTGGGCCTTAATTATTTTCATTCATTTTCGTTGTTGGAATCAGGCTGGAAGGCAAGGTGGCTTTGATAACCGGGGCAGCAAATGGGTTGGGCCAAGCCACAGCCCAAGAGTTCGTGGATCATGGGGGCCATGTGATAATTGCCGACATAGACACCACGTTGGGCCCACAGGTAGCCGAGCAACTGGGCCCCACCGCCAAATTCGTACAATGTGACGTGGCACTGGAGTCAAAAGTAGCCGCCGCCGTGAACTTTGTGGTGGCTCACCATGGAAAGTTGGACATTGTATACAACAACGCAGGAATCACCGGATCGATGCTGCCACAGAGCATAGCGGAGCTAGACCTAGCCGAGTTTGACCGAGTGATGAGCGTCAACGTGCGTGGAGTCATAGCCGGAATAAAGCACGCGGCTCGAGTTATGGTCCCGGCTGGGTCCGGGTCCATTTTGTGCACCTCAAGCATAAGTGGGCTCATGGGTGGGTTAGGCTCACTTCCTTATTCCATATCGAAACACGCAATTTCGGGGATAGTGAGGTCGGCGACGACGGAGCTCTGCCGGAGCGGAGTACGAGTGAACTGCATATCGCCGGCGCCGATAGCAACAACGCTGGCAGTGAAGGGGATAAGGGAAATGTACAAAGGGGATAGTAAGGAAGAGATCGTTGGAATAATAAATGGGCTTGGGGTTCTTAAGGGCGCAATTTGCGAGGAGAGTGACGTGGTGAAAGCGGCACTCTTTTTAGTTTCTGACGATGCCAAATACATCACCGGTCATAATCTCGTCGTCGACGGCGATCTTACTTCCTTCAAAAACCTCGATTTCCCTTCCTTCCACTAG

mRNA sequence

ATGTTCCGATCCATCGCCAGAGAGATTGAGATTATCGGTAATGGTCTTCTCAGTGGCCGTTTCAGGTCGTTTTCTTCGGTTTCCAGTCGGAGAAGGCTGGAAGGCAAGGTGGCTTTGATAACCGGGGCAGCAAATGGGTTGGGCCAAGCCACAGCCCAAGAGTTCGTGGATCATGGGGGCCATGTGATAATTGCCGACATAGACACCACGTTGGGCCCACAGGTAGCCGAGCAACTGGGCCCCACCGCCAAATTCGTACAATGTGACGTGGCACTGGAGTCAAAAGTAGCCGCCGCCGTGAACTTTGTGGTGGCTCACCATGGAAAGTTGGACATTGTATACAACAACGCAGGAATCACCGGATCGATGCTGCCACAGAGCATAGCGGAGCTAGACCTAGCCGAGTTTGACCGAGTGATGAGCGTCAACGTGCGTGGAGTCATAGCCGGAATAAAGCACGCGGCTCGAGTTATGGTCCCGGCTGGGTCCGGGTCCATTTTGTGCACCTCAAGCATAAGTGGGCTCATGGGTGGGTTAGGCTCACTTCCTTATTCCATATCGAAACACGCAATTTCGGGGATAGTGAGGTCGGCGACGACGGAGCTCTGCCGGAGCGGAGTACGAGTGAACTGCATATCGCCGGCGCCGATAGCAACAACGCTGGCAGTGAAGGGGATAAGGGAAATGTACAAAGGGGATAGTAAGGAAGAGATCGTTGGAATAATAAATGGGCTTGGGGTTCTTAAGGGCGCAATTTGCGAGGAGAGTGACGTGGTGAAAGCGGCACTCTTTTTAGTTTCTGACGATGCCAAATACATCACCGGTCATAATCTCGTCGTCGACGGCGATCTTACTTCCTTCAAAAACCTCGATTTCCCTTCCTTCCACTAG

Coding sequence (CDS)

ATGTTCCGATCCATCGCCAGAGAGATTGAGATTATCGGTAATGGTCTTCTCAGTGGCCGTTTCAGGTCGTTTTCTTCGGTTTCCAGTCGGAGAAGGCTGGAAGGCAAGGTGGCTTTGATAACCGGGGCAGCAAATGGGTTGGGCCAAGCCACAGCCCAAGAGTTCGTGGATCATGGGGGCCATGTGATAATTGCCGACATAGACACCACGTTGGGCCCACAGGTAGCCGAGCAACTGGGCCCCACCGCCAAATTCGTACAATGTGACGTGGCACTGGAGTCAAAAGTAGCCGCCGCCGTGAACTTTGTGGTGGCTCACCATGGAAAGTTGGACATTGTATACAACAACGCAGGAATCACCGGATCGATGCTGCCACAGAGCATAGCGGAGCTAGACCTAGCCGAGTTTGACCGAGTGATGAGCGTCAACGTGCGTGGAGTCATAGCCGGAATAAAGCACGCGGCTCGAGTTATGGTCCCGGCTGGGTCCGGGTCCATTTTGTGCACCTCAAGCATAAGTGGGCTCATGGGTGGGTTAGGCTCACTTCCTTATTCCATATCGAAACACGCAATTTCGGGGATAGTGAGGTCGGCGACGACGGAGCTCTGCCGGAGCGGAGTACGAGTGAACTGCATATCGCCGGCGCCGATAGCAACAACGCTGGCAGTGAAGGGGATAAGGGAAATGTACAAAGGGGATAGTAAGGAAGAGATCGTTGGAATAATAAATGGGCTTGGGGTTCTTAAGGGCGCAATTTGCGAGGAGAGTGACGTGGTGAAAGCGGCACTCTTTTTAGTTTCTGACGATGCCAAATACATCACCGGTCATAATCTCGTCGTCGACGGCGATCTTACTTCCTTCAAAAACCTCGATTTCCCTTCCTTCCACTAG

Protein sequence

MFRSIAREIEIIGNGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH

Homology

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BLAST of Lsi05G004500 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 125/270 (46.30%), Postives = 176/270 (65.19%), Query Frame = 0


Query: 31  RRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQL-----GPTAKF 90
           +RLEGKVA+ITG A+G+G+AT   F  HG  V+IAD+D   G  +A+ L      P   F
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 91  VQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLP-QSIAELDLAEFDRVMSVNV 150
           + CDV++E+ V   VN  VA +G+LDI++NNAG+ G     +SI + D  EFD VM VNV
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 151 RGVIAGIKHAARVMVPAG-SGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELC 210
           RGV  G+KH AR M+  G  G I+ T+S++G+MGG+G   Y+ SKHAI G+ ++A  EL 
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 211 RSGVRVNCISPAPIATTLAVKGIREMYKGDSK----EEIVGIINGLGVLKGAICEESDVV 270
           + G+RVNCISP  +AT++ V   R+   GD +    EE+   +  L  LKG     +D+ 
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 271 KAALFLVSDDAKYITGHNLVVDGDLTSFKN 290
           +AAL+L SD++KY+ GHNLVVDG +T+ +N
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGGVTTARN 299

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BLAST of Lsi05G004500 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.0e-58
Identity = 120/283 (42.40%), Postives = 172/283 (60.78%), Query Frame = 0


Query: 31  RRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLGPTAKFVQCDV 90
           +RL+GKVA++TG A G+G+A  + F  HG  V+IADID   G  +A  LGP   FV+CDV
Sbjct: 51  KRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGPQVSFVRCDV 110

Query: 91  ALESKVAAAVNFVVAHH-GKLDIVYNNAGITG--SMLPQSIAELDLAEFDRVMSVNVRGV 150
           ++E  V  AV++ ++ H G+LD+  NNAG+ G  +   +SI   D AEFDRV+ VN  G 
Sbjct: 111 SVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVNALGA 170

Query: 151 IAGIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGV 210
             G+KHAAR M P  +GSI+  +S++ ++GGLG   Y+ SKHAI G+ ++A  EL   GV
Sbjct: 171 ALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELRAHGV 230

Query: 211 RVNCISPAPIATTLAVKGIREMY--------------------KGDSKEEIVGIINGLGV 270
           RVNC+SP  +AT + +   R+ +                         E++  ++ GL  
Sbjct: 231 RVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVRGLAT 290

Query: 271 LKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNL 291
           LKG      D+ +A LFL SD+A+YI+GHNLVVDG +T+ +NL
Sbjct: 291 LKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNL 333

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BLAST of Lsi05G004500 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-54
Identity = 123/255 (48.24%), Postives = 167/255 (65.49%), Query Frame = 0


Query: 31  RRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLGPT-AKFVQCD 90
           RRLEGKVALITG A+G+G+ TA+ F  HG  V IAD+   LG  V E +G + + ++ CD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 91  VALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVRGVIA 150
           V  E  V  AV+  V+ +GKLDI+++NAGI+    P+ I + + A+F+RV SVNV GV  
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPR-IIDNEKADFERVFSVNVTGVFL 132

Query: 151 GIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGVRV 210
            +KHAARVM+PA SG+I+ T+S+S  MGG  S  Y  SKHA+ G+ R+   EL + G+RV
Sbjct: 133 CMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRV 192

Query: 211 NCISPAPIATTLAVK--GIREMYKGDSKEEIVGIINGLGVLKGAICEESDVVKAALFLVS 270
           NC+SP  + T L  K  GI+      ++EE   +IN  G LKG      DV  AAL+L S
Sbjct: 193 NCLSPFGLPTALGKKFSGIK------NEEEFENVINFAGNLKGPKFNVEDVANAALYLAS 252

Query: 271 DDAKYITGHNLVVDG 283
           D+AKY++GHNL +DG
Sbjct: 253 DEAKYVSGHNLFIDG 260

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BLAST of Lsi05G004500 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.0e-53
Identity = 121/257 (47.08%), Postives = 163/257 (63.42%), Query Frame = 0


Query: 31  RRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLGPTA-KFVQCD 90
           R+L GKVA+ITG A+G+G  TA+ FV HG  V++ADI   LG  +  +LGP A  +V CD
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCD 72

Query: 91  VALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVRGVIA 150
           V  E  VAAAV+  VA  GKLD+++NNAG++G    + ++E    +F+RV++VN+ G   
Sbjct: 73  VTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFR-MSECTKEDFERVLAVNLVGPFL 132

Query: 151 GIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGVRV 210
           G KHAARVM PA  GSI+ T+S+S  + G  S  Y+ SKHA+ G   +A  EL R G+RV
Sbjct: 133 GTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRV 192

Query: 211 NCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGA-ICEESDVVKAALFLVSD 270
           NC+SPA +AT LA   +     G   E I  I+     LKGA   +  D+  AALFL SD
Sbjct: 193 NCVSPAGVATPLARAAM-----GMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASD 252

Query: 271 DAKYITGHNLVVDGDLT 286
           D +Y++G NL VDG L+
Sbjct: 253 DGRYVSGQNLRVDGGLS 263

[Close]

BLAST of Lsi05G004500 vs. ExPASy Swiss-Prot
Match: F4J2Z7 (Short-chain dehydrogenase reductase 4 OS=Arabidopsis thaliana OX=3702 GN=SDR4 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-51
Identity = 116/261 (44.44%), Postives = 165/261 (63.22%), Query Frame = 0


Query: 30  RRRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLG-PTAKFVQC 89
           R+ L+GK+A+ITG A+G+G    + F DHG  V+I DI   LG  +A  +G   A F +C
Sbjct: 41  RQVLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRC 100

Query: 90  DVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVRGVI 149
           +V  E+ V  AV F V  HGKLD++++NAG+  +    S+ +LDL  FDR M+VNVRG  
Sbjct: 101 NVTDETDVENAVKFTVEKHGKLDVLFSNAGVLEAF--GSVLDLDLEAFDRTMAVNVRGAA 160

Query: 150 AGIKHAARVMVPAGS-GSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGV 209
           A IKHAAR MV +G+ GSI+CT+SI+  +GG G   Y+ SKHA+ G++RSA   L + G+
Sbjct: 161 AFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGI 220

Query: 210 RVNCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGAICEESDVVKAALFLVS 269
           RVN ++P  +AT     G+   Y  ++ + +      LG LKG + +   + +AALFL S
Sbjct: 221 RVNGVAPYGVAT-----GMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLAS 280

Query: 270 DDAKYITGHNLVVDGDLTSFK 289
           DD+ YI+G NLVVDG  +  K
Sbjct: 281 DDSVYISGQNLVVDGGFSVVK 294

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BLAST of Lsi05G004500 vs. ExPASy TrEMBL
Match: A0A0A0L8S5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G598890 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.3e-136
Identity = 258/297 (86.87%), Postives = 270/297 (90.91%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSS-RRRLEGKVALITGAANGLGQATAQEFVDHG 60
           M  S AREIE+IGNGLL G FRSFSSVSS RRRLEGKVALITGAANGLGQATAQEFVD G
Sbjct: 1   MLPSFAREIEMIGNGLLRGSFRSFSSVSSPRRRLEGKVALITGAANGLGQATAQEFVDQG 60

Query: 61  GHVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGI 120
            HVIIADIDTTLGPQVAEQLG TAKFV+CDVALES+VAAAVNF V HHGKLDI+YNNAGI
Sbjct: 61  AHVIIADIDTTLGPQVAEQLGHTAKFVECDVALESEVAAAVNFAVTHHGKLDIMYNNAGI 120

Query: 121 TGSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGL 180
           TG  +P SIAELDLA+FDRVM+VNVRGV+AGIKHAARVMVPAG GSILCTSSISGLMGGL
Sbjct: 121 TGPAVPPSIAELDLADFDRVMNVNVRGVVAGIKHAARVMVPAGCGSILCTSSISGLMGGL 180

Query: 181 GSLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIV 240
           G  PYSISKHAI GIVRSA TELCRSGVRVNCISPAP+AT +AVKGI EMYKG SKEEIV
Sbjct: 181 GPHPYSISKHAIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKGVSKEEIV 240

Query: 241 GIINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           GIINGLGVLKGAICEE+DV KAALFL  DD+KYITGHNLVVDG  TSFKNLDFPSFH
Sbjct: 241 GIINGLGVLKGAICEEADVAKAALFLACDDSKYITGHNLVVDGGFTSFKNLDFPSFH 297

[Close]

BLAST of Lsi05G004500 vs. ExPASy TrEMBL
Match: A0A6J1J856 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111482148 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.1e-134
Identity = 249/296 (84.12%), Postives = 266/296 (89.86%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGG 60
           M RS  REIEIIGN LL G FR+FSSVSSRRRL+GKVALITGAANGLG+ATAQEFVDHG 
Sbjct: 1   MLRSFVREIEIIGNDLLRGGFRAFSSVSSRRRLDGKVALITGAANGLGRATAQEFVDHGA 60

Query: 61  HVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGIT 120
           HVIIADIDTTLG Q AEQLGPTAKFVQCDVA+ES+VAAAVNF V HHGKLDI+YNNAGIT
Sbjct: 61  HVIIADIDTTLGSQAAEQLGPTAKFVQCDVAVESEVAAAVNFAVVHHGKLDIMYNNAGIT 120

Query: 121 GSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLG 180
           G  +P S+AELDLAEFDRVM VNVRGV+AGIKHAARVMVP+GSGSILCTSSISGLMGGLG
Sbjct: 121 GPAVPPSLAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPSGSGSILCTSSISGLMGGLG 180

Query: 181 SLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVG 240
             PYSISKHAI GIVR+A TELCRSGVRVNCISPAP+AT +AV GI E+YKG S+EEIVG
Sbjct: 181 PHPYSISKHAIPGIVRAAATELCRSGVRVNCISPAPVATAMAVSGIGEVYKGVSREEIVG 240

Query: 241 IINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           IINGLGVLKGA CEE DV KAAL+LVSDDAKYITGHNLVVDG  T FKNL+FPS H
Sbjct: 241 IINGLGVLKGAKCEEGDVAKAALYLVSDDAKYITGHNLVVDGGFTCFKNLEFPSLH 296

[Close]

BLAST of Lsi05G004500 vs. ExPASy TrEMBL
Match: A0A1S3BPB3 (momilactone A synthase-like OS=Cucumis melo OX=3656 GN=LOC103491733 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 251/287 (87.46%), Postives = 262/287 (91.29%), Query Frame = 0


Query: 11  IIGNGLLSGRFRSFSSVSS-RRRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDT 70
           ++GNGLL   FRSFSSVSS RRRLEGKVALITGAANGLGQATAQEFV+ G HVIIADID 
Sbjct: 1   MLGNGLLRRSFRSFSSVSSRRRRLEGKVALITGAANGLGQATAQEFVEQGAHVIIADIDN 60

Query: 71  TLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIA 130
           TLGPQVAEQLGPTAKFVQCDVALES+VAAAVNF V HHGKLDI+YNNAGITG  LP SIA
Sbjct: 61  TLGPQVAEQLGPTAKFVQCDVALESEVAAAVNFAVTHHGKLDIMYNNAGITGPALPPSIA 120

Query: 131 ELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKH 190
           ELDLA+FDRVMSVNVRGV+AGIKHAARVMVPAGSGSILCTSS+SGLMGGLG  PYSISKH
Sbjct: 121 ELDLADFDRVMSVNVRGVVAGIKHAARVMVPAGSGSILCTSSMSGLMGGLGPHPYSISKH 180

Query: 191 AISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLK 250
           AI GIVRSA TELCRSGVRVNCISPAP+AT +AVKGI EMYKG SKEEIVGIINGLGVLK
Sbjct: 181 AIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKGFSKEEIVGIINGLGVLK 240

Query: 251 GAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           GAICEE DV KAALFL SDD+KYITG NLVVDG  TSFKNLDFPSFH
Sbjct: 241 GAICEEVDVAKAALFLASDDSKYITGQNLVVDGGFTSFKNLDFPSFH 287

[Close]

BLAST of Lsi05G004500 vs. ExPASy TrEMBL
Match: A0A6J1F9M8 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111442080 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.3e-132
Identity = 246/296 (83.11%), Postives = 263/296 (88.85%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGG 60
           M RS AREIEIIGN LL G FR+FSS++SRRRL+GKVALITGAANGLG+ATAQEFVDHG 
Sbjct: 1   MLRSFAREIEIIGNDLLRGGFRAFSSLTSRRRLDGKVALITGAANGLGRATAQEFVDHGA 60

Query: 61  HVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGIT 120
           HV IADIDTTLG QVAEQLGPTAKFVQCDVA+ES+VAAAVNF V HHGKLDI+YNNAGIT
Sbjct: 61  HVFIADIDTTLGSQVAEQLGPTAKFVQCDVAVESEVAAAVNFAVVHHGKLDIMYNNAGIT 120

Query: 121 GSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLG 180
           G  +P SIAELDLAEFDRVM VNVR V+AGIKHAARVMVP GSGSILCTSSISGLMGGLG
Sbjct: 121 GPAVPPSIAELDLAEFDRVMRVNVRAVVAGIKHAARVMVPTGSGSILCTSSISGLMGGLG 180

Query: 181 SLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVG 240
             PYSISKHAI GIVR+  TELCRSGVRVNCISPAP+AT +AV GI E+YKG S+EEIVG
Sbjct: 181 PHPYSISKHAIPGIVRAVATELCRSGVRVNCISPAPVATAMAVSGIGEVYKGVSREEIVG 240

Query: 241 IINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           IINGLGVLKGA CEE DV KAAL+L SDDAKYITGHNLVVDG  T FKNL+FPS H
Sbjct: 241 IINGLGVLKGAKCEEGDVAKAALYLASDDAKYITGHNLVVDGGFTCFKNLEFPSLH 296

[Close]

BLAST of Lsi05G004500 vs. ExPASy TrEMBL
Match: A0A5D3E4K6 (Momilactone A synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold595G00170 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.1e-126
Identity = 237/271 (87.45%), Postives = 247/271 (91.14%), Query Frame = 0


Query: 26  SVSSRRRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLGPTAKF 85
           S SS + LEGKVALITGAANGLGQATAQEFV+ G HVIIADID TLGPQVAEQLGPTAKF
Sbjct: 3   SASSNQALEGKVALITGAANGLGQATAQEFVEQGAHVIIADIDNTLGPQVAEQLGPTAKF 62

Query: 86  VQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVR 145
           VQCDVALES+VAAAVNF V HHGKLDI+YNNAGITG  LP SIAELDLA+FDRVMSVNVR
Sbjct: 63  VQCDVALESEVAAAVNFAVTHHGKLDIMYNNAGITGPALPPSIAELDLADFDRVMSVNVR 122

Query: 146 GVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRS 205
           GV+AGIKHAARVMVPAGSGSILCTSS+SGLMGGLG  PYSISKHAI GIVRSA TELCR 
Sbjct: 123 GVVAGIKHAARVMVPAGSGSILCTSSMSGLMGGLGPHPYSISKHAIPGIVRSAATELCRC 182

Query: 206 GVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGAICEESDVVKAALFL 265
           GVRVNCISPAP+AT +AVKGI EMYKG SKEEIVGIINGLGVLKGAICEE DV KAALFL
Sbjct: 183 GVRVNCISPAPVATAMAVKGIGEMYKGFSKEEIVGIINGLGVLKGAICEEVDVAKAALFL 242

Query: 266 VSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
            SDD+KYITGHNLVVDG  TSFKNLDFPSFH
Sbjct: 243 ASDDSKYITGHNLVVDGGFTSFKNLDFPSFH 273

[Close]

BLAST of Lsi05G004500 vs. NCBI nr
Match: XP_011651586.1 (short-chain dehydrogenase reductase 3c [Cucumis sativus] >KGN58243.1 hypothetical protein Csa_017609 [Cucumis sativus])

HSP 1 Score: 495.7 bits (1275), Expect = 2.7e-136
Identity = 258/297 (86.87%), Postives = 270/297 (90.91%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSS-RRRLEGKVALITGAANGLGQATAQEFVDHG 60
           M  S AREIE+IGNGLL G FRSFSSVSS RRRLEGKVALITGAANGLGQATAQEFVD G
Sbjct: 1   MLPSFAREIEMIGNGLLRGSFRSFSSVSSPRRRLEGKVALITGAANGLGQATAQEFVDQG 60

Query: 61  GHVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGI 120
            HVIIADIDTTLGPQVAEQLG TAKFV+CDVALES+VAAAVNF V HHGKLDI+YNNAGI
Sbjct: 61  AHVIIADIDTTLGPQVAEQLGHTAKFVECDVALESEVAAAVNFAVTHHGKLDIMYNNAGI 120

Query: 121 TGSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGL 180
           TG  +P SIAELDLA+FDRVM+VNVRGV+AGIKHAARVMVPAG GSILCTSSISGLMGGL
Sbjct: 121 TGPAVPPSIAELDLADFDRVMNVNVRGVVAGIKHAARVMVPAGCGSILCTSSISGLMGGL 180

Query: 181 GSLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIV 240
           G  PYSISKHAI GIVRSA TELCRSGVRVNCISPAP+AT +AVKGI EMYKG SKEEIV
Sbjct: 181 GPHPYSISKHAIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKGVSKEEIV 240

Query: 241 GIINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           GIINGLGVLKGAICEE+DV KAALFL  DD+KYITGHNLVVDG  TSFKNLDFPSFH
Sbjct: 241 GIINGLGVLKGAICEEADVAKAALFLACDDSKYITGHNLVVDGGFTSFKNLDFPSFH 297

[Close]

BLAST of Lsi05G004500 vs. NCBI nr
Match: XP_023527081.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488.0 bits (1255), Expect = 5.6e-134
Identity = 250/296 (84.46%), Postives = 265/296 (89.53%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGG 60
           M RS AREIEIIGN LL G FR+FSSVSSRRRL+GKVALITGAANGLG+ATAQEFVDHG 
Sbjct: 1   MLRSFAREIEIIGNDLLRGGFRAFSSVSSRRRLDGKVALITGAANGLGRATAQEFVDHGA 60

Query: 61  HVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGIT 120
           HVIIADIDTTLG QVAEQLGPTAKFVQCDVA+ES+VAAAVNF V HHGKLDI+YNNAGIT
Sbjct: 61  HVIIADIDTTLGSQVAEQLGPTAKFVQCDVAVESEVAAAVNFAVVHHGKLDIMYNNAGIT 120

Query: 121 GSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLG 180
           G  LP SIAELDLAEFDRVM VNVRGV+AGIKHAARVMVP GSGSILCTSSISGLMGGLG
Sbjct: 121 GPALPPSIAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPTGSGSILCTSSISGLMGGLG 180

Query: 181 SLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVG 240
             PYSISKHAI GIVR+  TELCRSGVRVNCISPAP+AT +AV GI E+YKG S+EEI+G
Sbjct: 181 PHPYSISKHAIPGIVRAVATELCRSGVRVNCISPAPVATAMAVSGIGEVYKGVSREEIIG 240

Query: 241 IINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           IINGLGVLKGA CEE DV KAAL+L SDDAKYITGHNLVVDG  T FKNL+FPS H
Sbjct: 241 IINGLGVLKGAKCEEGDVAKAALYLASDDAKYITGHNLVVDGGFTCFKNLEFPSLH 296

[Close]

BLAST of Lsi05G004500 vs. NCBI nr
Match: XP_022983588.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 249/296 (84.12%), Postives = 266/296 (89.86%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGG 60
           M RS  REIEIIGN LL G FR+FSSVSSRRRL+GKVALITGAANGLG+ATAQEFVDHG 
Sbjct: 1   MLRSFVREIEIIGNDLLRGGFRAFSSVSSRRRLDGKVALITGAANGLGRATAQEFVDHGA 60

Query: 61  HVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGIT 120
           HVIIADIDTTLG Q AEQLGPTAKFVQCDVA+ES+VAAAVNF V HHGKLDI+YNNAGIT
Sbjct: 61  HVIIADIDTTLGSQAAEQLGPTAKFVQCDVAVESEVAAAVNFAVVHHGKLDIMYNNAGIT 120

Query: 121 GSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLG 180
           G  +P S+AELDLAEFDRVM VNVRGV+AGIKHAARVMVP+GSGSILCTSSISGLMGGLG
Sbjct: 121 GPAVPPSLAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPSGSGSILCTSSISGLMGGLG 180

Query: 181 SLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVG 240
             PYSISKHAI GIVR+A TELCRSGVRVNCISPAP+AT +AV GI E+YKG S+EEIVG
Sbjct: 181 PHPYSISKHAIPGIVRAAATELCRSGVRVNCISPAPVATAMAVSGIGEVYKGVSREEIVG 240

Query: 241 IINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           IINGLGVLKGA CEE DV KAAL+LVSDDAKYITGHNLVVDG  T FKNL+FPS H
Sbjct: 241 IINGLGVLKGAKCEEGDVAKAALYLVSDDAKYITGHNLVVDGGFTCFKNLEFPSLH 296

[Close]

BLAST of Lsi05G004500 vs. NCBI nr
Match: KAG6581134.1 (Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 485.0 bits (1247), Expect = 4.8e-133
Identity = 248/296 (83.78%), Postives = 265/296 (89.53%), Query Frame = 0


Query: 1   MFRSIAREIEIIGNGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGG 60
           M RS AREIEIIGN LL G FR+FSS++SRRRL+GKVALITGAANGLG+ATAQEFVDHG 
Sbjct: 29  MLRSFAREIEIIGNDLLRGGFRAFSSLTSRRRLDGKVALITGAANGLGRATAQEFVDHGA 88

Query: 61  HVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGIT 120
           HVIIADIDTTLG QVAEQLGPTAKFVQCDVA+ES+VAAAVNF V HHGKLDI+YNNAGIT
Sbjct: 89  HVIIADIDTTLGSQVAEQLGPTAKFVQCDVAVESEVAAAVNFAVVHHGKLDIMYNNAGIT 148

Query: 121 GSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLG 180
           G  +P SIAELDLAEFDRVM VNVRGV+AGIKHAARVMVP GSGSILCTSSISGLMGGLG
Sbjct: 149 GPAVPPSIAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPTGSGSILCTSSISGLMGGLG 208

Query: 181 SLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVG 240
             PYSISKHAI GIVR+  TELCRSGVRVNCISPAP+AT +AV GI E+YKG S+EEIVG
Sbjct: 209 PHPYSISKHAIPGIVRAVATELCRSGVRVNCISPAPVATAMAVSGIGEVYKGVSREEIVG 268

Query: 241 IINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           IINGLGVLKGA CEE DV KAAL+L SDDAKYITGHNLVVDG  T FKNL+FPS H
Sbjct: 269 IINGLGVLKGAKCEEGDVAKAALYLASDDAKYITGHNLVVDGGFTCFKNLEFPSLH 324

[Close]

BLAST of Lsi05G004500 vs. NCBI nr
Match: XP_008450017.1 (PREDICTED: momilactone A synthase-like [Cucumis melo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.1e-132
Identity = 251/287 (87.46%), Postives = 262/287 (91.29%), Query Frame = 0


Query: 11  IIGNGLLSGRFRSFSSVSS-RRRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDT 70
           ++GNGLL   FRSFSSVSS RRRLEGKVALITGAANGLGQATAQEFV+ G HVIIADID 
Sbjct: 1   MLGNGLLRRSFRSFSSVSSRRRRLEGKVALITGAANGLGQATAQEFVEQGAHVIIADIDN 60

Query: 71  TLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIA 130
           TLGPQVAEQLGPTAKFVQCDVALES+VAAAVNF V HHGKLDI+YNNAGITG  LP SIA
Sbjct: 61  TLGPQVAEQLGPTAKFVQCDVALESEVAAAVNFAVTHHGKLDIMYNNAGITGPALPPSIA 120

Query: 131 ELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKH 190
           ELDLA+FDRVMSVNVRGV+AGIKHAARVMVPAGSGSILCTSS+SGLMGGLG  PYSISKH
Sbjct: 121 ELDLADFDRVMSVNVRGVVAGIKHAARVMVPAGSGSILCTSSMSGLMGGLGPHPYSISKH 180

Query: 191 AISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLK 250
           AI GIVRSA TELCRSGVRVNCISPAP+AT +AVKGI EMYKG SKEEIVGIINGLGVLK
Sbjct: 181 AIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKGFSKEEIVGIINGLGVLK 240

Query: 251 GAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPSFH 297
           GAICEE DV KAALFL SDD+KYITG NLVVDG  TSFKNLDFPSFH
Sbjct: 241 GAICEEVDVAKAALFLASDDSKYITGQNLVVDGGFTSFKNLDFPSFH 287

[Close]

BLAST of Lsi05G004500 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 320.1 bits (819), Expect = 1.9e-87
Identity = 169/299 (56.52%), Postives = 216/299 (72.24%), Query Frame = 0


Query: 1   MFRSIAREIEIIG--NGLLSGRFRSFSSVS-SRRRLEGKVALITGAANGLGQATAQEFVD 60
           M+RS AR  ++I   NGL+S   RS    S S R+LEGKVA+ITG A+G+G+ATA+EFV 
Sbjct: 1   MWRSFARSFKLINVPNGLISKPIRSTLLYSTSSRKLEGKVAVITGGASGIGKATAEEFVS 60

Query: 61  HGGHVIIADIDTTLGPQVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNA 120
            G  VII DID   G  VA +LG  A F++CDV  E ++A AV   V  HGKLD++ N+A
Sbjct: 61  QGAQVIIVDIDEEAGHMVATELGSAAHFLRCDVTEEEQIAKAVETAVTRHGKLDVMLNSA 120

Query: 121 GITGSMLPQSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMG 180
           GI+ S+ P SIA+LD+  +D+VM +NVRG + GIKHAAR M+PAGSGSILC SSISGLMG
Sbjct: 121 GISCSISPPSIADLDMDTYDKVMRLNVRGTVLGIKHAARAMIPAGSGSILCLSSISGLMG 180

Query: 181 GLGSLPYSISKHAISGIVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDS--K 240
           GLG   YSISK  I G+V++  +ELC+ G+R+NCISPA I T L ++  RE + G S  +
Sbjct: 181 GLGPHAYSISKFTIPGVVKTVASELCKHGLRINCISPAGIPTPLTLRMFREAFAGHSIRE 240

Query: 241 EEIVGIINGLGVLKGAICEESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPS 295
           E+++ I+N  G LKG  CEE DV KAAL+L SDDAK++TGHNLVVDG  T FK+L+ PS
Sbjct: 241 EQLLAIVNATGELKGEKCEEIDVAKAALYLASDDAKFVTGHNLVVDGGFTCFKSLNLPS 299

[Close]

BLAST of Lsi05G004500 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 6.3e-83
Identity = 147/263 (55.89%), Postives = 199/263 (75.67%), Query Frame = 0


Query: 31  RRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLGPTAKFVQCDV 90
           ++LEGKVALITG A+GLG+ATA EF+ HG  V+IAD+D   G + A++LG  A+FV+CDV
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSEAEFVRCDV 98

Query: 91  ALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVRGVIAG 150
            +E+ +A AV   V  +GKLD++YNNAGI G M P SI++LD+ EF+RVM +NV GV++G
Sbjct: 99  TVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVSG 158

Query: 151 IKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGVRVN 210
           IKHAA+ M+PA SG ILCTSS++G+ GGL    Y+ISK    GIV+SA +ELC  GVR+N
Sbjct: 159 IKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRIN 218

Query: 211 CISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGAICEESDVVKAALFLVSDDA 270
           CISP  +AT L +  +++++   S+E++   + G+G LKGA CEE+DV KAAL+L S+D 
Sbjct: 219 CISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALYLASNDG 278

Query: 271 KYITGHNLVVDGDLTSFKNLDFP 294
           KY+TGHNLVVDG +T+FK   FP
Sbjct: 279 KYVTGHNLVVDGGMTAFKIAGFP 301

[Close]

BLAST of Lsi05G004500 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.5e-81
Identity = 150/281 (53.38%), Postives = 199/281 (70.82%), Query Frame = 0


Query: 14  NGLLSGRFRSFSSVSSRRRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGP 73
           N L+ G   S SS S+ R+LEGKVALITG A+G+G+ATA +F+ HG  VIIADI   +G 
Sbjct: 59  NFLIKGISSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGR 118

Query: 74  QVAEQLGPTAKFVQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDL 133
           +  ++LGP+  +  CDV  ES +A AV+F V+ H KLDI+YNNAGI     P SI +LDL
Sbjct: 119 ETEQELGPSCAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIP-CKTPPSIVDLDL 178

Query: 134 AEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLGSLPYSISKHAISG 193
             FD+V++ NVRGV+AGIKHAARVM+P  SGSI+C  S++G+MGGL    YS+SK A+ G
Sbjct: 179 NVFDKVINTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIG 238

Query: 194 IVRSATTELCRSGVRVNCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGAIC 253
           IVRS  +ELC+  +RVNCISP  I T+  +  +R++Y G     ++ I+   GVL G +C
Sbjct: 239 IVRSTASELCKHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVC 298

Query: 254 EESDVVKAALFLVSDDAKYITGHNLVVDGDLTSFKNLDFPS 295
           E +DV  AA++L SDD+KY+ GHNLVVDG  T+ K LDFP+
Sbjct: 299 EPTDVANAAVYLASDDSKYVNGHNLVVDGGFTTVKTLDFPA 338

[Close]

BLAST of Lsi05G004500 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-61
Identity = 125/270 (46.30%), Postives = 176/270 (65.19%), Query Frame = 0


Query: 31  RRLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQL-----GPTAKF 90
           +RLEGKVA+ITG A+G+G+AT   F  HG  V+IAD+D   G  +A+ L      P   F
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 91  VQCDVALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLP-QSIAELDLAEFDRVMSVNV 150
           + CDV++E+ V   VN  VA +G+LDI++NNAG+ G     +SI + D  EFD VM VNV
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 151 RGVIAGIKHAARVMVPAG-SGSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELC 210
           RGV  G+KH AR M+  G  G I+ T+S++G+MGG+G   Y+ SKHAI G+ ++A  EL 
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 211 RSGVRVNCISPAPIATTLAVKGIREMYKGDSK----EEIVGIINGLGVLKGAICEESDVV 270
           + G+RVNCISP  +AT++ V   R+   GD +    EE+   +  L  LKG     +D+ 
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 271 KAALFLVSDDAKYITGHNLVVDGDLTSFKN 290
           +AAL+L SD++KY+ GHNLVVDG +T+ +N
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGGVTTARN 299

[Close]

BLAST of Lsi05G004500 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 116/259 (44.79%), Postives = 164/259 (63.32%), Query Frame = 0


Query: 32  RLEGKVALITGAANGLGQATAQEFVDHGGHVIIADIDTTLGPQVAEQLG-PTAKFVQCDV 91
           RL+GK+A+ITG A+G+G    + F DHG  V+I DI   LG  +A  +G   A F +C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 92  ALESKVAAAVNFVVAHHGKLDIVYNNAGITGSMLPQSIAELDLAEFDRVMSVNVRGVIAG 151
             E+ V  AV F V  HGKLD++++NAG+  +    S+ +LDL  FDR M+VNVRG  A 
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAF--GSVLDLDLEAFDRTMAVNVRGAAAF 124

Query: 152 IKHAARVMVPAGS-GSILCTSSISGLMGGLGSLPYSISKHAISGIVRSATTELCRSGVRV 211
           IKHAAR MV +G+ GSI+CT+SI+  +GG G   Y+ SKHA+ G++RSA   L + G+RV
Sbjct: 125 IKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRV 184

Query: 212 NCISPAPIATTLAVKGIREMYKGDSKEEIVGIINGLGVLKGAICEESDVVKAALFLVSDD 271
           N ++P  +AT     G+   Y  ++ + +      LG LKG + +   + +AALFL SDD
Sbjct: 185 NGVAPYGVAT-----GMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDD 244

Query: 272 AKYITGHNLVVDGDLTSFK 289
           + YI+G NLVVDG  +  K
Sbjct: 245 SVYISGQNLVVDGGFSVVK 256

The following BLAST results are available for this feature:

BLAST of Lsi05G004500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCU0	1.5e-60	46.30	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
P50160	4.0e-58	42.40	Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1	[more]
Q94KL7	1.2e-54	48.24	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	3.0e-53	47.08	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
F4J2Z7	2.8e-51	44.44	Short-chain dehydrogenase reductase 4 OS=Arabidopsis thaliana OX=3702 GN=SDR4 PE...	[more]

BLAST of Lsi05G004500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8S5	1.3e-136	86.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G598890 PE=4 SV=1	[more]
A0A6J1J856	6.1e-134	84.12	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A1S3BPB3	1.5e-132	87.46	momilactone A synthase-like OS=Cucumis melo OX=3656 GN=LOC103491733 PE=4 SV=1	[more]
A0A6J1F9M8	3.3e-132	83.11	short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5D3E4K6	2.1e-126	87.45	Momilactone A synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of Lsi05G004500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651586.1	2.7e-136	86.87	short-chain dehydrogenase reductase 3c [Cucumis sativus] >KGN58243.1 hypothetica...	[more]
XP_023527081.1	5.6e-134	84.46	short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983588.1	1.3e-133	84.12	short-chain dehydrogenase reductase 2a-like [Cucurbita maxima]	[more]
KAG6581134.1	4.8e-133	83.78	Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_008450017.1	3.1e-132	87.46	PREDICTED: momilactone A synthase-like [Cucumis melo]	[more]

BLAST of Lsi05G004500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26760.1	1.9e-87	56.52	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	6.3e-83	55.89	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	3.5e-81	53.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	1.0e-61	46.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	1.2e-52	44.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 164..172 score: 38.19 coord: 184..203 score: 30.22 coord: 108..119 score: 51.07
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 108..119 score: 57.66 coord: 184..203 score: 31.95 coord: 248..268 score: 25.82 coord: 37..54 score: 47.77 coord: 158..174 score: 32.34 coord: 205..222 score: 36.77
None	No IPR available	SMART	SM00822	PKS_KR	coord: 36..215 e-value: 0.0092 score: -60.6
None	No IPR available	GENE3D	3.40.50.720		coord: 24..291 e-value: 2.8E-71 score: 241.7
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 42..282 e-value: 2.8E-58 score: 197.2
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 39..231 e-value: 5.3E-19 score: 66.3
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 33..282 e-value: 9.8E-19 score: 65.3
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 19..294
None	No IPR available	PANTHER	PTHR43180:SF55	ALCOHOL DEHYDROGENASE-LIKE PROTEIN	coord: 19..294
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 34..283

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G004500.1	Lsi05G004500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity