Lsi05G000460 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G000460 vs. ExPASy Swiss-Prot
Match: Q9FJR9 (Nuclear intron maturase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT2 PE=1 SV=1)

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 556/708 (78.53%), Postives = 630/708 (88.98%), Query Frame = 0

Query: 22  SNSFLFSKLNPLNSSA-HGFSLCRVFSFVPPHRR-----TPDPDDPSTLMKEDGVSVCSQ 81
           +N + F    PL S+  +G ++ R  S+  P +R      PDPDDP+ L+KEDGVS+CSQ
Sbjct: 21  TNVYCFVTNPPLISNPNNGSAIFRYLSYFAPQQRRQQQQAPDPDDPANLLKEDGVSLCSQ 80

Query: 82  MWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRNAIQRSALEDLLALRN 141
           MW+ENF+EPD+  +NL +YLRRFELWVLAYQKVC DE+GAY+PR++IQRSALE+LLALRN
Sbjct: 81  MWLENFKEPDKTATNLTSYLRRFELWVLAYQKVCCDELGAYVPRSSIQRSALENLLALRN 140

Query: 142 AVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAAFQDKIVQEVLLMILE 201
           +VLD +FKWG+RL+F+IKSP+DKTDYESLSKRKIKA+LTTTQP  FQD+IVQEVLLMILE
Sbjct: 141 SVLDDRFKWGSRLDFYIKSPRDKTDYESLSKRKIKAILTTTQPTPFQDRIVQEVLLMILE 200

Query: 202 PIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILDGMKVGMVINALMR 261
           PIYE+RFS KS+AFRPGR AHTVLRVIRR+FAGYLWYVKGDLS +LDGMKVG VI++LMR
Sbjct: 201 PIYESRFSQKSFAFRPGRTAHTVLRVIRRNFAGYLWYVKGDLSVVLDGMKVGFVISSLMR 260

Query: 262 DIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAEDEPKPDPYWLETFFG 321
           D+RDK+VIDLIKSALVTPV+TSK+++GEKKK KKRKYQKKRVLAEDEPKPDPYWLETFFG
Sbjct: 261 DVRDKKVIDLIKSALVTPVVTSKVEDGEKKKTKKRKYQKKRVLAEDEPKPDPYWLETFFG 320

Query: 322 FAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQ 381
           FAPEEA K+P WGHCGILSPLL N+CLDELDRWME ++KDFY PSKSDVIWN+PEGEADQ
Sbjct: 321 FAPEEAGKSPQWGHCGILSPLLVNVCLDELDRWMETKVKDFYRPSKSDVIWNNPEGEADQ 380

Query: 382 GNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKELIEFCDQKYMLKLD 441
           GNTSWPEFVPTSGPDKTRKMDY+RYGGHILIG+RGPRADAATLRKELIEF DQKYML+LD
Sbjct: 381 GNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGVRGPRADAATLRKELIEFVDQKYMLRLD 440

Query: 442 NECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVGTLLSVTASLKQCIKQ 501
           NE LPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVGTLLSVTASLKQCIKQ
Sbjct: 441 NENLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVGTLLSVTASLKQCIKQ 500

Query: 502 FRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYRYADNRRKVVNFCSYI 561
           FRKL F+KGDRDPDPQPCFRMFHATQAHTN+QMNKFL TI EWYR+ADNR+K+VNFCSYI
Sbjct: 501 FRKLLFIKGDRDPDPQPCFRMFHATQAHTNNQMNKFLTTIAEWYRFADNRKKIVNFCSYI 560

Query: 562 LRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYNLLRMGLAESIDGLKF 621
           +RGSLAKLYAAKYKLRSRAKVYK   RNLS PL +KKGQSPEY NLLRMGLAES+DGL +
Sbjct: 561 IRGSLAKLYAAKYKLRSRAKVYKFANRNLSLPLLQKKGQSPEYQNLLRMGLAESVDGLVY 620

Query: 622 TRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCIREQGLVSPQEYISML 681
           TRMSLVPETDY+P P NWRP+HEK L+E++ L++P+TLEEQ+R IRE+GLVSPQ+Y SML
Sbjct: 621 TRMSLVPETDYSPFPGNWRPEHEKFLIEYLTLDEPKTLEEQKRFIREKGLVSPQDYTSML 680

Query: 682 VWNYKRNA-SMDQTSLMNSGDHRLLGS----NLDSHDSKAKELEEHDE 719
           VWNYKRNA  MDQ S++      LLGS    N D+ D K KE +E  E
Sbjct: 681 VWNYKRNAIPMDQVSILKDQPF-LLGSSSTYNRDNDDQKNKEEDEDSE 727

BLAST of Lsi05G000460 vs. ExPASy Swiss-Prot
Match: Q9C8R8 (Nuclear intron maturase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT1 PE=1 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 1.4e-210
Identity = 352/647 (54.40%), Postives = 482/647 (74.50%), Query Frame = 0

Query: 53  RRTPDPDDPSTLMKEDGVSVCSQMWIENFRE-PDRIVSNLITYLRRFELWVLAYQKVCAD 112
           R      DP +L+K+D V +C  +W+++F   P    SNL  +L +F+LWVLAYQ+ CA 
Sbjct: 41  REPSSTQDPYSLLKQDPVDICLSLWVKSFSSPPSATFSNLTGFLSKFDLWVLAYQRTCAH 100

Query: 113 EMGAYMPRNAIQRSALEDLLALRNAVLDS--KFKWGARLEFFIKSPKDKTDY---ESLSK 172
             G + PRNAI  +AL  LL+L+NAV  S  KF+W  ++  +++SPKDK      E +SK
Sbjct: 101 VTGTFPPRNAIHANALRSLLSLQNAVTRSGGKFRWNDKMNQYVRSPKDKISMNGGEGMSK 160

Query: 173 RKIKAMLTTTQPAAFQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHF 232
            K++ ++ + +P  FQD++V EVLLMILEP +EARFS KS+ FRPGRN HTV+R IR +F
Sbjct: 161 GKVRRIIESEEP-IFQDRVVHEVLLMILEPFFEARFSSKSHGFRPGRNPHTVIRTIRSNF 220

Query: 233 AGYLWYVKGDLSTILDGMKVGMVINALMRDIRDKQVIDLIKSAL------VTPVITSKID 292
           AGYLW++KGD+S +LD + V +V+N L + ++D++V+ LI+S+L      V   +  K  
Sbjct: 221 AGYLWFMKGDVSEMLDHVDVDVVMNCLQKVVKDRKVLGLIESSLKFSDKRVLKRVVEKHG 280

Query: 293 E----GEKKK--KKKRKYQKKRVLAEDEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILS 352
                G K++  ++KR   KK++L++DEPKPDPYWL TF+ FAP+EA K PS+G+CG+LS
Sbjct: 281 NDNGLGTKRRIEREKRNKTKKKILSDDEPKPDPYWLRTFYSFAPKEAAKVPSYGYCGVLS 340

Query: 353 PLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRK 412
           PLLAN+CL+ELDR+ME +I +++SP K D IW     E    N +WPEFVP+SG +KTRK
Sbjct: 341 PLLANVCLNELDRFMETKIVEYFSPCKDDSIWKE-SIEDGCHNPAWPEFVPSSGKEKTRK 400

Query: 413 MDYIRYGGHILIGIRGPRADAATLRKELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHV 472
           MDYIRYGGH LIGIRGPR DA  +RKE+I+FCD+ + ++LDN  L IEHI++GI FLDH+
Sbjct: 401 MDYIRYGGHFLIGIRGPREDAVKMRKEIIDFCDRVFGVRLDNSKLEIEHISRGIQFLDHI 460

Query: 473 LCRRVVYPTLRYTASGGKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCF 532
           +CRRV+YPTLRYT SGG I+S+KGVGTLLSV+ASL+QCI+QFR+L F+KGD+DP+P PC 
Sbjct: 461 ICRRVIYPTLRYTGSGGSIVSKKGVGTLLSVSASLEQCIRQFRRLAFVKGDKDPEPLPCN 520

Query: 533 RMFHATQAHTNSQMNKFLLTIVEWYRYADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRA 592
            M +++Q+H+NSQMNKFL T+ +WY+YADNR+K V FC+Y++R SLAKLYAA+Y+L+SRA
Sbjct: 521 PMLYSSQSHSNSQMNKFLETMADWYKYADNRKKAVGFCAYVIRSSLAKLYAARYRLKSRA 580

Query: 593 KVYKIGARNLSRPLKEKKGQS-PEYYNLLRMGLAESIDGLKFTRMSLVPETDYTPLPNNW 652
           KVY I +R+LS PL E    S PEY +LLRMGL ++I+G++F+RMSL+P  DYTP P NW
Sbjct: 581 KVYSIASRDLSHPLSESSNNSAPEYSDLLRMGLVDAIEGVQFSRMSLIPSCDYTPFPRNW 640

Query: 653 RPDHEKALLEFIMLEDPRTLEEQRRCIREQGLVSPQEYISMLVWNYK 681
            P+HE+ L E+I L+DP+      R I+ +GL  PQ+ IS  VW++K
Sbjct: 641 IPNHEQVLQEYIRLQDPKFFCGLHRSIKREGLTLPQDEISEAVWDFK 685

BLAST of Lsi05G000460 vs. ExPASy Swiss-Prot
Match: P38456 (Uncharacterized mitochondrial protein ymf11 OS=Marchantia polymorpha OX=3197 GN=YMF11 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 4.7e-36
Identity = 133/524 (25.38%), Postives = 216/524 (41.22%), Query Frame = 0

Query: 182 QDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTIL 241
           +D +VQEV+  ILE +YE  F   S+ FRPGR+ HT L+ IRR F G +W+++G+ S   
Sbjct: 243 KDILVQEVIRSILETLYEPYFLSCSHGFRPGRSQHTCLKQIRRDFVGTVWFIEGETSQYF 302

Query: 242 DGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAED 301
           + +   ++I  + R IRD + ++L++  + T +       G                   
Sbjct: 303 NKIDKQVLIGLMRRRIRDNRFLNLVQKEIKTSLRAGAEGTG------------------- 362

Query: 302 EPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWM--------EGRI 361
                                          + PLL NI L ELD ++         GR 
Sbjct: 363 -------------------------------VGPLLCNIVLHELDLFVMRLKRIVDRGRR 422

Query: 362 KDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSG--------PDKTRKMDYIRYGGHIL 421
           +     SK   +W       D+           SG        P +TR+++Y+R+    L
Sbjct: 423 RAVNPESKE--LWRQSAALIDRTTAHRARVPFPSGAFGRGLGHPQETRQINYVRFADDFL 482

Query: 422 IGIRGPRADAATLRKELIEFCDQKYMLKLDNE-----------CLPIEHITKG------- 481
           IG+ GPRA A  +R  +  F + +  L+L  +            LP  ++  G       
Sbjct: 483 IGVIGPRALAERIRGLVTRFIEVRLKLRLTLDKTRKPIQSRPNTLPAHYVPMGPKETGPK 542

Query: 482 --------------------------IMFLDHVLCRRVVYPTLRYTASGGKIISEKGVGT 541
                                     I FL +++ R   + T      G +    +  G 
Sbjct: 543 VENDFTISGGPGKKKTQIFCITKNKKIPFLGYLISRDSKH-TYNLVRRGRRYRIRRSGG- 602

Query: 542 LLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYRY 601
            LS+   +++ I +  +  F   D+   P+P F  F   Q+++ +++   L  +  +Y  
Sbjct: 603 -LSLLVDMQKVINRLAEKGFC--DKSGHPKPNFAYFQYPQSYSVARIASILRGLANYYHL 662

Query: 602 ADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYNL 636
           A+++R+ V   SYILR SLAK YAAK+KL + AKV+  G R+LS+P+K KK + P    +
Sbjct: 663 ANSKRQCVTRLSYILRTSLAKTYAAKFKLGTAAKVFAKGGRDLSKPIKAKKARRPLLNRV 709

BLAST of Lsi05G000460 vs. ExPASy Swiss-Prot
Match: P0A3U0 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=1359 GN=ltrA PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 5.8e-26
Identity = 136/553 (24.59%), Postives = 226/553 (40.87%), Query Frame = 0

Query: 85  DRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRNAIQRSALEDLLALRNAVLDSKFKW 144
           D + + L  YL R +++ +AYQ +       Y  + A  +  L+D     +   + K K 
Sbjct: 20  DEVFTRLYRYLLRPDIYYVAYQNL-------YSNKGASTKGILDD---TADGFSEEKIK- 79

Query: 145 GARLEFFIKSPKDKTDYES------LSKRKIKAMLTTTQPAAFQDKIVQEVLLMILEPIY 204
                  I+S KD T Y        ++K+  K M     P  F DK++QE + +ILE IY
Sbjct: 80  -----KIIQSLKDGTYYPQPVRRMYIAKKNSKKMRPLGIP-TFTDKLIQEAVRIILESIY 139

Query: 205 EARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILDGMKVGMVINALMRDIR 264
           E  F   S+ FRP R+ HT L+ I+R F G  W+V+GD+    D +    +I  +   I+
Sbjct: 140 EPVFEDVSHGFRPQRSCHTALKTIKREFGGARWFVEGDIKGCFDNIDHVTLIGLINLKIK 199

Query: 265 DKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAEDEPKPDPYWLETFFGFAP 324
           D ++  LI   L    +            +  +Y K                 T+ G  P
Sbjct: 200 DMKMSQLIYKFLKAGYL------------ENWQYHK-----------------TYSG-TP 259

Query: 325 EEAVKNPSWGHCGILSPLLANICLDELDRW-----------------------------M 384
           +           GILSPLLANI L ELD++                             +
Sbjct: 260 QG----------GILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRI 319

Query: 385 EGRIKDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIR 444
             R+K      K+ V+      E  +     P    TS  +K  K  Y+RY    +I ++
Sbjct: 320 SHRLKKLEGEEKAKVLL-----EYQEKRKRLPTLPCTSQTNKVLK--YVRYADDFIISVK 379

Query: 445 GPRADAATLRKELIEFCDQKYMLKLDNECLPIEHITKGIMFLDH-VLCRRVVYPTLRYTA 504
           G + D   ++++L  F   K  ++L  E   I H ++   FL + +  RR    T++ + 
Sbjct: 380 GSKEDCQWIKEQLKLFIHNKLKMELSEEKTLITHSSQPARFLGYDIRVRR--SGTIKRSG 439

Query: 505 SGGKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQM 564
              K      V  L+ +   ++Q I   +K+   K D    P     +  +T     +  
Sbjct: 440 KVKKRTLNGSVELLIPLQDKIRQFIFD-KKIAIQKKDSSWFPVHRKYLIRSTDLEIITIY 499

Query: 565 NKFLLTIVEWYRYADNRRKVVNFCSYILRGSLAKLYAAKYK--LRSRAKVYKIGARNLSR 600
           N  L  I  +Y  A N  + +N+ +Y++  S  K  A+K+K  L     ++K G+ +   
Sbjct: 500 NSELRGICNYYGLASNFNQ-LNYFAYLMEYSCLKTIASKHKGTLSKTISMFKDGSGSWGI 504

BLAST of Lsi05G000460 vs. ExPASy Swiss-Prot
Match: P0A3U1 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=ltrA PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 5.8e-26
Identity = 136/553 (24.59%), Postives = 226/553 (40.87%), Query Frame = 0

Query: 85  DRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRNAIQRSALEDLLALRNAVLDSKFKW 144
           D + + L  YL R +++ +AYQ +       Y  + A  +  L+D     +   + K K 
Sbjct: 20  DEVFTRLYRYLLRPDIYYVAYQNL-------YSNKGASTKGILDD---TADGFSEEKIK- 79

Query: 145 GARLEFFIKSPKDKTDYES------LSKRKIKAMLTTTQPAAFQDKIVQEVLLMILEPIY 204
                  I+S KD T Y        ++K+  K M     P  F DK++QE + +ILE IY
Sbjct: 80  -----KIIQSLKDGTYYPQPVRRMYIAKKNSKKMRPLGIP-TFTDKLIQEAVRIILESIY 139

Query: 205 EARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILDGMKVGMVINALMRDIR 264
           E  F   S+ FRP R+ HT L+ I+R F G  W+V+GD+    D +    +I  +   I+
Sbjct: 140 EPVFEDVSHGFRPQRSCHTALKTIKREFGGARWFVEGDIKGCFDNIDHVTLIGLINLKIK 199

Query: 265 DKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAEDEPKPDPYWLETFFGFAP 324
           D ++  LI   L    +            +  +Y K                 T+ G  P
Sbjct: 200 DMKMSQLIYKFLKAGYL------------ENWQYHK-----------------TYSG-TP 259

Query: 325 EEAVKNPSWGHCGILSPLLANICLDELDRW-----------------------------M 384
           +           GILSPLLANI L ELD++                             +
Sbjct: 260 QG----------GILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRI 319

Query: 385 EGRIKDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIR 444
             R+K      K+ V+      E  +     P    TS  +K  K  Y+RY    +I ++
Sbjct: 320 SHRLKKLEGEEKAKVLL-----EYQEKRKRLPTLPCTSQTNKVLK--YVRYADDFIISVK 379

Query: 445 GPRADAATLRKELIEFCDQKYMLKLDNECLPIEHITKGIMFLDH-VLCRRVVYPTLRYTA 504
           G + D   ++++L  F   K  ++L  E   I H ++   FL + +  RR    T++ + 
Sbjct: 380 GSKEDCQWIKEQLKLFIHNKLKMELSEEKTLITHSSQPARFLGYDIRVRR--SGTIKRSG 439

Query: 505 SGGKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQM 564
              K      V  L+ +   ++Q I   +K+   K D    P     +  +T     +  
Sbjct: 440 KVKKRTLNGSVELLIPLQDKIRQFIFD-KKIAIQKKDSSWFPVHRKYLIRSTDLEIITIY 499

Query: 565 NKFLLTIVEWYRYADNRRKVVNFCSYILRGSLAKLYAAKYK--LRSRAKVYKIGARNLSR 600
           N  L  I  +Y  A N  + +N+ +Y++  S  K  A+K+K  L     ++K G+ +   
Sbjct: 500 NSELRGICNYYGLASNFNQ-LNYFAYLMEYSCLKTIASKHKGTLSKTISMFKDGSGSWGI 504

BLAST of Lsi05G000460 vs. ExPASy TrEMBL
Match: A0A6J1EYF8 (nuclear intron maturase 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111439589 PE=4 SV=1)

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 691/725 (95.31%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN FLFSKLNPL+ S HGFS+CRVFSFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLR+IRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRIIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITSKIDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFY+PS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYAPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           REQGLVSPQ+YISMLVWNYKRNASMDQTSLMN+ DHR+LGSNL S  SKAKELEEHDE+F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNASMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 725

BLAST of Lsi05G000460 vs. ExPASy TrEMBL
Match: A0A6J1I719 (nuclear intron maturase 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111471814 PE=4 SV=1)

HSP 1 Score: 1405.2 bits (3636), Expect = 0.0e+00
Identity = 688/725 (94.90%), Postives = 706/725 (97.38%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN  LFSKLNPLN S HGFS+CRVFSFVP HRRTP PDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGLLFSKLNPLNPSFHGFSVCRVFSFVPAHRRTPGPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCAD+MGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADDMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITS IDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSNIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFR+LNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRRLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
            EQGLVSPQ+YISMLVWNYKRNA+MDQTSLMN+ DHR+LGSNLDS  SKAKELEEHDE+F
Sbjct: 661 SEQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLDSFGSKAKELEEHDESF 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 725

BLAST of Lsi05G000460 vs. ExPASy TrEMBL
Match: A0A5D3E763 (Putative mitochondrial protein ymf11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004040 PE=4 SV=1)

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 684/725 (94.34%), Postives = 704/725 (97.10%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFAHRIFASSSVVRTS+ FLFS+LNPL SS HGF LCRVFSF+P HRR PDPDD
Sbjct: 1   MHRGVSIFAHRIFASSSVVRTSSGFLFSELNPLKSSFHGFPLCRVFSFMPAHRRAPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PS L+KEDG+SVCSQMWIENFREPDRIVSNL TYLRRFELWVLAYQKVC DEMGAYMPRN
Sbjct: 61  PSNLLKEDGISVCSQMWIENFREPDRIVSNLTTYLRRFELWVLAYQKVCTDEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDS+FKWGARLEFFIKSPKDKTDY +LSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSRFKWGARLEFFIKSPKDKTDYGALSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFS KSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSSKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG VINALMRDIRDK+VIDLIKSALVTPVITSKIDEGE KKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGAVINALMRDIRDKKVIDLIKSALVTPVITSKIDEGE-KKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEG+IKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGKIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCD+KYMLKLD+ECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG
Sbjct: 421 QLIEFCDEKYMLKLDSECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWY+
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYK 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQR CI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRSCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           RE GLVSPQ+YISMLVWNYKRNA+MDQ SLMNSGDHR+LG NLDSHDSK+KELEEHD   
Sbjct: 661 RELGLVSPQDYISMLVWNYKRNATMDQMSLMNSGDHRILGLNLDSHDSKSKELEEHD--- 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 721

BLAST of Lsi05G000460 vs. ExPASy TrEMBL
Match: A0A1S3CPZ6 (uncharacterized mitochondrial protein ymf11 OS=Cucumis melo OX=3656 GN=LOC103503499 PE=4 SV=1)

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 684/725 (94.34%), Postives = 704/725 (97.10%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFAHRIFASSSVVRTS+ FLFS+LNPL SS HGF LCRVFSF+P HRR PDPDD
Sbjct: 1   MHRGVSIFAHRIFASSSVVRTSSGFLFSELNPLKSSFHGFPLCRVFSFMPAHRRAPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PS L+KEDG+SVCSQMWIENFREPDRIVSNL TYLRRFELWVLAYQKVC DEMGAYMPRN
Sbjct: 61  PSNLLKEDGISVCSQMWIENFREPDRIVSNLTTYLRRFELWVLAYQKVCTDEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDS+FKWGARLEFFIKSPKDKTDY +LSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSRFKWGARLEFFIKSPKDKTDYGALSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFS KSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSSKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG VINALMRDIRDK+VIDLIKSALVTPVITSKIDEGE KKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGAVINALMRDIRDKKVIDLIKSALVTPVITSKIDEGE-KKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEG+IKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGKIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCD+KYMLKLD+ECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG
Sbjct: 421 QLIEFCDEKYMLKLDSECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWY+
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYK 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQR CI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRSCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           RE GLVSPQ+YISMLVWNYKRNA+MDQ SLMNSGDHR+LG NLDSHDSK+KELEEHD   
Sbjct: 661 RELGLVSPQDYISMLVWNYKRNATMDQMSLMNSGDHRILGLNLDSHDSKSKELEEHD--- 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 721

BLAST of Lsi05G000460 vs. ExPASy TrEMBL
Match: A0A6J1E311 (nuclear intron maturase 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111026013 PE=4 SV=1)

HSP 1 Score: 1387.5 bits (3590), Expect = 0.0e+00
Identity = 677/725 (93.38%), Postives = 705/725 (97.24%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVS+FAHRIFASSS+VRTSN F+FSKLNP NSS +GFS CRVFSFVPPHRRTPDPDD
Sbjct: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVFSFVPPHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRIV+NL +YLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKA+LTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMR IRDK+VIDL+KSALVTPVITSK+DEGEKKKKKKRKYQKKRVLAE
Sbjct: 241 LDGMKVGLVINALMRGIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPR LEEQ RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRMLEEQLRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           RE+GLVS Q+YISMLVWNYKRNA++DQT LMNSGD RLLGS+LD    KAKE+EEHD+AF
Sbjct: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720

Query: 721 QAAEV 726
           + AEV
Sbjct: 721 RVAEV 725

BLAST of Lsi05G000460 vs. NCBI nr
Match: XP_023542306.1 (nuclear intron maturase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1412.9 bits (3656), Expect = 0.0e+00
Identity = 692/725 (95.45%), Postives = 710/725 (97.93%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN FLFSKLNPLN S HGFS+CRVFSFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLNPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLSTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITSKIDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDP+TLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPKTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           REQGLVSPQ+YISMLVWNYKRNA+MDQTSLMN+ DHR+LGSNL+S  SKAKELEEHDE+F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLESLGSKAKELEEHDESF 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 725

BLAST of Lsi05G000460 vs. NCBI nr
Match: KAG7033617.1 (Nuclear intron maturase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1411.4 bits (3652), Expect = 0.0e+00
Identity = 692/725 (95.45%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN FLFSKLNPL+ S HGFS+CRVFSFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITSKIDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           REQGLVSPQ+YISMLVWNYKRNA+MDQTSLMN+ DHR+LGSNL S  SKAKELEEHDE+F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 725

BLAST of Lsi05G000460 vs. NCBI nr
Match: XP_022932969.1 (nuclear intron maturase 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 691/725 (95.31%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN FLFSKLNPL+ S HGFS+CRVFSFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLR+IRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRIIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITSKIDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFY+PS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYAPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           REQGLVSPQ+YISMLVWNYKRNASMDQTSLMN+ DHR+LGSNL S  SKAKELEEHDE+F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNASMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 725

BLAST of Lsi05G000460 vs. NCBI nr
Match: KAG6571755.1 (Nuclear intron maturase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 691/725 (95.31%), Postives = 708/725 (97.66%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN FLFSKLNPL+ S HGFS+CRVFSFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITSKIDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
           REQGLVSPQ+YISMLVWNYKRNA+MDQTSLMN+ DHR+LGSNL S  SKAKELEEHDE+F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 QAAEV 726
            AAEV
Sbjct: 721 HAAEV 725

BLAST of Lsi05G000460 vs. NCBI nr
Match: XP_022973257.1 (nuclear intron maturase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1405.2 bits (3636), Expect = 0.0e+00
Identity = 688/725 (94.90%), Postives = 706/725 (97.38%), Query Frame = 0

Query: 1   MHRGVSIFAHRIFASSSVVRTSNSFLFSKLNPLNSSAHGFSLCRVFSFVPPHRRTPDPDD 60
           MHRGVSIFA RIF SS VV TSN  LFSKLNPLN S HGFS+CRVFSFVP HRRTP PDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGLLFSKLNPLNPSFHGFSVCRVFSFVPAHRRTPGPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI+SNL TYLRRFELWVLAYQKVCAD+MGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADDMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAA 180
           AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKA+LTTTQPAA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVL MILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGMVINALMRDIRDK+VIDL+K+ALVTPVITS IDEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSNIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 ELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480
           +LIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFR+LNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRRLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYN 600
           YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEY+N
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660
           LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEE RRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REQGLVSPQEYISMLVWNYKRNASMDQTSLMNSGDHRLLGSNLDSHDSKAKELEEHDEAF 720
            EQGLVSPQ+YISMLVWNYKRNA+MDQTSLMN+ DHR+LGSNLDS  SKAKELEEHDE+F
Sbjct: 661 SEQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLDSFGSKAKELEEHDESF 720

Query: 721 QAAEV 726
           QAAEV
Sbjct: 721 QAAEV 725

BLAST of Lsi05G000460 vs. TAIR 10
Match: AT5G46920.1 (Intron maturase, type II family protein )

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 556/708 (78.53%), Postives = 630/708 (88.98%), Query Frame = 0

Query: 22  SNSFLFSKLNPLNSSA-HGFSLCRVFSFVPPHRR-----TPDPDDPSTLMKEDGVSVCSQ 81
           +N + F    PL S+  +G ++ R  S+  P +R      PDPDDP+ L+KEDGVS+CSQ
Sbjct: 21  TNVYCFVTNPPLISNPNNGSAIFRYLSYFAPQQRRQQQQAPDPDDPANLLKEDGVSLCSQ 80

Query: 82  MWIENFREPDRIVSNLITYLRRFELWVLAYQKVCADEMGAYMPRNAIQRSALEDLLALRN 141
           MW+ENF+EPD+  +NL +YLRRFELWVLAYQKVC DE+GAY+PR++IQRSALE+LLALRN
Sbjct: 81  MWLENFKEPDKTATNLTSYLRRFELWVLAYQKVCCDELGAYVPRSSIQRSALENLLALRN 140

Query: 142 AVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAMLTTTQPAAFQDKIVQEVLLMILE 201
           +VLD +FKWG+RL+F+IKSP+DKTDYESLSKRKIKA+LTTTQP  FQD+IVQEVLLMILE
Sbjct: 141 SVLDDRFKWGSRLDFYIKSPRDKTDYESLSKRKIKAILTTTQPTPFQDRIVQEVLLMILE 200

Query: 202 PIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILDGMKVGMVINALMR 261
           PIYE+RFS KS+AFRPGR AHTVLRVIRR+FAGYLWYVKGDLS +LDGMKVG VI++LMR
Sbjct: 201 PIYESRFSQKSFAFRPGRTAHTVLRVIRRNFAGYLWYVKGDLSVVLDGMKVGFVISSLMR 260

Query: 262 DIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAEDEPKPDPYWLETFFG 321
           D+RDK+VIDLIKSALVTPV+TSK+++GEKKK KKRKYQKKRVLAEDEPKPDPYWLETFFG
Sbjct: 261 DVRDKKVIDLIKSALVTPVVTSKVEDGEKKKTKKRKYQKKRVLAEDEPKPDPYWLETFFG 320

Query: 322 FAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQ 381
           FAPEEA K+P WGHCGILSPLL N+CLDELDRWME ++KDFY PSKSDVIWN+PEGEADQ
Sbjct: 321 FAPEEAGKSPQWGHCGILSPLLVNVCLDELDRWMETKVKDFYRPSKSDVIWNNPEGEADQ 380

Query: 382 GNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKELIEFCDQKYMLKLD 441
           GNTSWPEFVPTSGPDKTRKMDY+RYGGHILIG+RGPRADAATLRKELIEF DQKYML+LD
Sbjct: 381 GNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGVRGPRADAATLRKELIEFVDQKYMLRLD 440

Query: 442 NECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVGTLLSVTASLKQCIKQ 501
           NE LPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVGTLLSVTASLKQCIKQ
Sbjct: 441 NENLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVGTLLSVTASLKQCIKQ 500

Query: 502 FRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYRYADNRRKVVNFCSYI 561
           FRKL F+KGDRDPDPQPCFRMFHATQAHTN+QMNKFL TI EWYR+ADNR+K+VNFCSYI
Sbjct: 501 FRKLLFIKGDRDPDPQPCFRMFHATQAHTNNQMNKFLTTIAEWYRFADNRKKIVNFCSYI 560

Query: 562 LRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYYNLLRMGLAESIDGLKF 621
           +RGSLAKLYAAKYKLRSRAKVYK   RNLS PL +KKGQSPEY NLLRMGLAES+DGL +
Sbjct: 561 IRGSLAKLYAAKYKLRSRAKVYKFANRNLSLPLLQKKGQSPEYQNLLRMGLAESVDGLVY 620

Query: 622 TRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCIREQGLVSPQEYISML 681
           TRMSLVPETDY+P P NWRP+HEK L+E++ L++P+TLEEQ+R IRE+GLVSPQ+Y SML
Sbjct: 621 TRMSLVPETDYSPFPGNWRPEHEKFLIEYLTLDEPKTLEEQKRFIREKGLVSPQDYTSML 680

Query: 682 VWNYKRNA-SMDQTSLMNSGDHRLLGS----NLDSHDSKAKELEEHDE 719
           VWNYKRNA  MDQ S++      LLGS    N D+ D K KE +E  E
Sbjct: 681 VWNYKRNAIPMDQVSILKDQPF-LLGSSSTYNRDNDDQKNKEEDEDSE 727

BLAST of Lsi05G000460 vs. TAIR 10
Match: AT1G30010.1 (Intron maturase, type II family protein )

HSP 1 Score: 734.2 bits (1894), Expect = 1.0e-211
Identity = 352/647 (54.40%), Postives = 482/647 (74.50%), Query Frame = 0

Query: 53  RRTPDPDDPSTLMKEDGVSVCSQMWIENFRE-PDRIVSNLITYLRRFELWVLAYQKVCAD 112
           R      DP +L+K+D V +C  +W+++F   P    SNL  +L +F+LWVLAYQ+ CA 
Sbjct: 41  REPSSTQDPYSLLKQDPVDICLSLWVKSFSSPPSATFSNLTGFLSKFDLWVLAYQRTCAH 100

Query: 113 EMGAYMPRNAIQRSALEDLLALRNAVLDS--KFKWGARLEFFIKSPKDKTDY---ESLSK 172
             G + PRNAI  +AL  LL+L+NAV  S  KF+W  ++  +++SPKDK      E +SK
Sbjct: 101 VTGTFPPRNAIHANALRSLLSLQNAVTRSGGKFRWNDKMNQYVRSPKDKISMNGGEGMSK 160

Query: 173 RKIKAMLTTTQPAAFQDKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHF 232
            K++ ++ + +P  FQD++V EVLLMILEP +EARFS KS+ FRPGRN HTV+R IR +F
Sbjct: 161 GKVRRIIESEEP-IFQDRVVHEVLLMILEPFFEARFSSKSHGFRPGRNPHTVIRTIRSNF 220

Query: 233 AGYLWYVKGDLSTILDGMKVGMVINALMRDIRDKQVIDLIKSAL------VTPVITSKID 292
           AGYLW++KGD+S +LD + V +V+N L + ++D++V+ LI+S+L      V   +  K  
Sbjct: 221 AGYLWFMKGDVSEMLDHVDVDVVMNCLQKVVKDRKVLGLIESSLKFSDKRVLKRVVEKHG 280

Query: 293 E----GEKKK--KKKRKYQKKRVLAEDEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILS 352
                G K++  ++KR   KK++L++DEPKPDPYWL TF+ FAP+EA K PS+G+CG+LS
Sbjct: 281 NDNGLGTKRRIEREKRNKTKKKILSDDEPKPDPYWLRTFYSFAPKEAAKVPSYGYCGVLS 340

Query: 353 PLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRK 412
           PLLAN+CL+ELDR+ME +I +++SP K D IW     E    N +WPEFVP+SG +KTRK
Sbjct: 341 PLLANVCLNELDRFMETKIVEYFSPCKDDSIWKE-SIEDGCHNPAWPEFVPSSGKEKTRK 400

Query: 413 MDYIRYGGHILIGIRGPRADAATLRKELIEFCDQKYMLKLDNECLPIEHITKGIMFLDHV 472
           MDYIRYGGH LIGIRGPR DA  +RKE+I+FCD+ + ++LDN  L IEHI++GI FLDH+
Sbjct: 401 MDYIRYGGHFLIGIRGPREDAVKMRKEIIDFCDRVFGVRLDNSKLEIEHISRGIQFLDHI 460

Query: 473 LCRRVVYPTLRYTASGGKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCF 532
           +CRRV+YPTLRYT SGG I+S+KGVGTLLSV+ASL+QCI+QFR+L F+KGD+DP+P PC 
Sbjct: 461 ICRRVIYPTLRYTGSGGSIVSKKGVGTLLSVSASLEQCIRQFRRLAFVKGDKDPEPLPCN 520

Query: 533 RMFHATQAHTNSQMNKFLLTIVEWYRYADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRA 592
            M +++Q+H+NSQMNKFL T+ +WY+YADNR+K V FC+Y++R SLAKLYAA+Y+L+SRA
Sbjct: 521 PMLYSSQSHSNSQMNKFLETMADWYKYADNRKKAVGFCAYVIRSSLAKLYAARYRLKSRA 580

Query: 593 KVYKIGARNLSRPLKEKKGQS-PEYYNLLRMGLAESIDGLKFTRMSLVPETDYTPLPNNW 652
           KVY I +R+LS PL E    S PEY +LLRMGL ++I+G++F+RMSL+P  DYTP P NW
Sbjct: 581 KVYSIASRDLSHPLSESSNNSAPEYSDLLRMGLVDAIEGVQFSRMSLIPSCDYTPFPRNW 640

Query: 653 RPDHEKALLEFIMLEDPRTLEEQRRCIREQGLVSPQEYISMLVWNYK 681
            P+HE+ L E+I L+DP+      R I+ +GL  PQ+ IS  VW++K
Sbjct: 641 IPNHEQVLQEYIRLQDPKFFCGLHRSIKREGLTLPQDEISEAVWDFK 685

BLAST of Lsi05G000460 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-05
Identity = 56/253 (22.13%), Postives = 103/253 (40.71%), Query Frame = 0

Query: 184 KIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLS-TILD 243
           K++ E + M+LE +Y+ RF+  SY  R G   HT +R ++       W+ +   +  + +
Sbjct: 137 KVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFE 196

Query: 244 GMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAEDE 303
              V ++   +   I D  +I++IK      ++  KI+ G     +              
Sbjct: 197 ERNVDILCGFVGEKINDVMLIEMIKKLFEFGIL--KIELGGCNSGR-------------- 256

Query: 304 PKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKS 363
                       GF P+E         CG+ S +L N+  D LD+     I+D     K 
Sbjct: 257 ------------GF-PQE---------CGLCS-ILINVYFDGLDK----EIQDLRLKMK- 316

Query: 364 DVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKEL 423
             + N   G  D+ +T    F P +       +  +RY   IL+   G +     L+K +
Sbjct: 317 --VKNPRVGTGDEESTGNVFFKPVN-------IYAVRYLDEILVITSGSKMLTMDLKKRI 336

Query: 424 IEFCDQKYMLKLD 436
           ++  +Q+  L++D
Sbjct: 377 VDILEQRLELRVD 336

BLAST of Lsi05G000460 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-05
Identity = 56/253 (22.13%), Postives = 103/253 (40.71%), Query Frame = 0

Query: 184 KIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLS-TILD 243
           K++ E + M+LE +Y+ RF+  SY  R G   HT +R ++       W+ +   +  + +
Sbjct: 137 KVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFE 196

Query: 244 GMKVGMVINALMRDIRDKQVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKRVLAEDE 303
              V ++   +   I D  +I++IK      ++  KI+ G     +              
Sbjct: 197 ERNVDILCGFVGEKINDVMLIEMIKKLFEFGIL--KIELGGCNSGR-------------- 256

Query: 304 PKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKS 363
                       GF P+E         CG+ S +L N+  D LD+     I+D     K 
Sbjct: 257 ------------GF-PQE---------CGLCS-ILINVYFDGLDK----EIQDLRLKMK- 316

Query: 364 DVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKEL 423
             + N   G  D+ +T    F P +       +  +RY   IL+   G +     L+K +
Sbjct: 317 --VKNPRVGTGDEESTGNVFFKPVN-------IYAVRYLDEILVITSGSKMLTMDLKKRI 336

Query: 424 IEFCDQKYMLKLD 436
           ++  +Q+  L++D
Sbjct: 377 VDILEQRLELRVD 336

BLAST of Lsi05G000460 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 45.1 bits (105), Expect = 2.9e-04
Identity = 24/77 (31.17%), Postives = 42/77 (54.55%), Query Frame = 0

Query: 183 DKIVQEVLLMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILD 242
           +KI++E + M+LE IY+  F P +  FR G+  H+VLR I+  +    W+++ D+     
Sbjct: 14  EKIMKEAIRMVLESIYDPEF-PDTSHFRSGQGCHSVLRRIKEEWGISRWFLEFDIRKCFH 73

Query: 243 GMKVGMVINALMRDIRD 260
            +    +I  L  +I D
Sbjct: 74  TIDRHRLIQILKEEIDD 89