Lsi04G020310 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G020310
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionsodium/calcium exchanger NCL-like
Locationchr04: 27412429 .. 27417455 (-)
RNA-Seq ExpressionLsi04G020310
SyntenyLsi04G020310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAATTATTAAATTCTTTCATCTTCCTCACAGAGAGGATCGTCCTCTCAACGTTCTCACACGGAAACACGGTTGCAAAGCTGATCCCAACAGTTTCAGTCCCAATCCAACCAATCCTTCTTTTAAGCTACACCCACCACTTTCTTCCTTAAACCCACTTCTTCTCTCTCGCCGGACCTCCAGATTTTGCCTCAAAAGCCATGTCCACCACTCCCCTGCATCTCCTCCTCCTCCTTTTCCTCATCCTCTGTGCTCAATCCCACAGCTCCCGCCTCGCTTCCATTTCTTCCCTCACTTCTTCCTCCTCCTCTGATCTGGTCTCCGATGGCGTCAATGAAATGCAGGAATCCCCTTATCTGCATCTCAACACGCTTTCTTCATTCTCTGCACCCGAGGAGGAGTCTTGCGAGCAGTCTTATGGGTTTCTTCCCTGTACCACCACTGCTTTGGGGAATCTCTTTTTGATTATCGTTTATGGCTATTTGATGTTCTTGGCTGCTAAATACTTGTCTACCGGAAGCGAGCTATTGTTGGAGATCTTGGGACCTGGAATTGTGGGGGGATTGTTCCTTCCCGCGCTTGGTGCTCTTCCTGATGCTATGCTCATTCTCGGTACTTGTTGCTTCTTTCGATTTTTTTTCTTCTTTATTTCTTTTGCACTTTCATGAGTTTTGACCTGTAATCGAAATGTTCTGCGAAATGAACGTTTTGTCGAGAACATGATCATTTTGATTTGTGAATTTGAGGTGTGGTTCTGTTTTTATGATCCAGGCGAAGGTTAGTAAATTGGGTAGGTGTCTCTGGAAAATAAGTGATAGATCTGTATTTTACTTTCATTTTTCATTTGTTCATTTCCCCCATGAACTGTTTGATCGCAAAAACTGAATCAGGTATTATTACATAAACGCGGTGGAAACAGAATCGGAATCGTGAAATGTATAGTGGGTCTCTTTTGTTTTTTCTTGGCTACTTTTTCTAAAAAAGTTTCGGTAATTGACCACCAAAAGTTTCATAAATGGTTTGAGTATTATAAAATAAAGGTGGTGGTGCTTGTTGTTGAAGTTCTATTTGCTGTTATGCCTCTACTTTGTTGTGTTTGGTTTTGCGTGGCCTGGCATCTCTCTGCTTTTGCTGGAGCTCACTTTTCTGGCTATTCCTTGGTTCAAACTTTATCGTACTTCAATTTTTTCTGTTTGTCGGTTAGACCTCAACATCTTTTTTCTTTTTTACGAGAGACCTCAACATCAAGAACCTGAACGATTCTCACCTCTTTTCCTTGAACTGACATCCTTTCAAAATCTCAGCCATTAGTTCCCAAACTGGGCACGATTTATTCCAAAATTTCAGTTTAGCCATTTCTCCCTCCCTATGTTTTCAAAATGGACCATCCTCTAATATCTGTTGTCTTTTGTTTCTTGAGCCGTTTTTAAATGATGGGTTATGTAATTCTCAACTCCATATTTTTTGGGTTTATATTTTATATCGATTTTTTTTCTTTTTTTTTTTTTTTTGGCAAAATGATATCTAGACTTTCACCAATGGAAGTTTGGTTAAATGACCTTCTTCGAATATGAAGGGTGATAAAAAGCATCGAGTTCCTAAGTTTGAATTGGCCACTCTCATTGGAATGTTTATTGTTTTGATTTCTCTTGTTCCAATTTGTTAGTGACAAATGACAGATATGATAAAACCCTCTAATCTTCTCCTTTCTATAGTTTCTGGTCTTGCAGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCTGTTGGAATGGGCCTCCTAGCTGGCTCAACTGTCATGCTTCTAACACTGATCTGGGGTACATGTGTGATTGTTGGGAAGTGTGACCTTCAGGATTCAGTTGCCATAGATTCACAGGATACAAAAGGGTTTAGCTTGACTGGTATGCCATTTTATCTTAGATATATATTCAACTGTTGTATAAAATGTACCTTTTCCATATGTTTTCTTTATCACTTTTGCACCCGAACAAATTTCAAGGTGTCTGTCTGTGTTCCTGTATGCCTTTGTTTTTAGAAGGGGAGTTGAATCTTATTCATCGGAGTGAGATGTTTTCTTATGGTGTGGAAACAATGATGATGCTATTCGTAATGGATCAAGACTTTTTTTCTTCTTTTTCTTTTTTTTTTTTTTTTTTTGCAACATAGGAGGAGGGTTTTGAACCTTAGACTTTTTGGTTGAGAGTACACTCTTTATACCAGTTGAACTATGCTCACTTTAGCGGATAGTCAAGACATACAAAGAAAAAAGATATTTTTTTAATAGTATTAAAGGTGTTATGTGAGTTCTGTCATTGACCACAGGCAACTTCTATAGTTTTTTTTAGTCGTGACTTGTGACAATGGCAATTTCTCGTTTTATCTACTCTTCCTATTGCATAGAAACTGAAGATTAGATAATCTTGGTGAAAATATATGCAAGCATCAATTAAAGCGTGAAACTTACATAGTCTTTCATGTTGGGAAGACACCAGTTTTTCATCTCATCAACATACCTAAAACTGTTAGTACATGGTTGCCTCTTAATATTTCATCATTGCACTTCCATGTGATTTCGTTCTGAGGGTCAACTATTCTCAGTCACTGGTCCATTTGCTCACACTTGCTATTGGGTCACCTGAATTGGTAGTGAATTTTCAAATGTCATATGGTTTTATATGTATAAGATATATTCTATCAATTGGCTAGTAAAAGTTCTGTTCTTCATGTACATATAATCGATGCATAAATTTCAATTGGAATTTCTTCTTTGACAGAATCTGGAGTCAGTACTGATATTTGGACCAGCTATGCTGCCAGGATTATGGTTATATCTGTTTTTCCATTTTTAATAGTTCAACTTCCACAAATGCTCAATTCAACATCAGGAAGGCACTTGGCAGTGTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATCTATTGTCTTTACCAGGTGCTTTTTCTATTTCTTAGCTCTTTCTTTTTTTTTTTTTCTTTTTTTTTTATACAGTTCACCCAAAATTTTATTTTCATTTTTTGGAATTTGTTGTCCACACAATATTGGAAAAAAAATTAAAAGATCCACATTTACAGGTTGTCATGTTTTAAACATAAATGCTAACTTGAATAAGCTTACCTTCAATTCAAACATGCCGTATTCGGTGAGCAAGAAACATAGATAGCTATTCTGATCTTCCAGAGTTCAAAAGTTATAGCTGTAATGGAAGGGTCAAGACCTTCACAATGTATTGGCGCGCAATGTAACTTTGTTCTGTCAAACTTGATATAGACCTTTTAATTTAATGATAAGGCATTTAGTTTCTTGTATCTTTGATCTTTTGCCATTTTTTTTTTCTTTTCTCATTTATTGATGTTTAAAATTGTACCTCAACGATATCCTATTTCATGAGTTGAGAAAATTTGTGCAGGTCTTCCAGCCATGGATTCAAAGGAGGAAACTTGCCTTTGTCAAGCACAAACATGTTATATTTGGACTTTTGAGACATTTAAAACAGCAAGCACTTGGAAGGCTCCTGACAGAAAATGGGGAGCCAGATAAAGAAATAATAGAGAAGTTCGTTGGAAAAAGAAGTTCAATTGTTTATTTTTATCATCGCCTTAATATTTTATCAGATTCTGATATTTGCTCAATGAACATAATGGCAGGTTATTTTCAAGAATTGATGAGAACAGGGATGGACTTCTTTCAGCTTCTGAACTGAGAGCACTAATTGTTGGCATTCAATTTGAGGAGATCGACTTAGATCATGATGATGCTGTTGTGAAAATACTGAATGACTTCGATACTTCTCGTGATTCCCATGTTGATGCTACTGAGTTTGGCAATGGGATTATTAGGTGGCTTTCTCAAGTACAAGGTTCAAGAACTGGCCGAGGTGATGATGGTCCTCACACGATGAAGTTCCTGCATAACTTTCATCAAGTAAGTTGTAATCTGTTTGCTGCAATTCTCCTTTCGACTTTTTATCTCGCTTTTCTGAAGCATGTGTGATGTATCTACAGGAAACAAAGAGAGAGCATGATTTGCTGGATGTCGGGGAACAGAGTGATGAGGTCGTAGAGGGCGTTGAGGGTGGCAAGGGGGTCTTGATCAAAGCTATACTCTTCTTGTTAATTGGAACTGCCATTGCTGCTGCCTTTGCTGACCCTTTGGTCGACGTCGTGCATAACTTCTCCAATGCAACTAGCATCCCTGCGTTCTTTATTTCATTTATCGCTCTGCCATTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACTGCCTCGTTGACATTTTCTGAGGTTTGTTCTCTTTTATTCTCTTTTATACCTAACATTTCATTTCTTTAGTCATAGTTCGTTTTTGCTGGGGACTGGTAGAAAAGAAAATAGGAAGGATTTGGAAGAATGATTGATCTCTAAGCTTTCATGGTGCAGCTATATGGAGCAGTGACAATGAACAATGTCCTCTGCCTATCAGTCTTCTTGGCTCTTGTTTACGTGAGAGGATTGGTTTGGAACTTTTCATCTGAAGTGCTGGTCATTCTCGTCGTCACGATTATAATGGGAGTTCTCGGTAGCTTTCGAACCGCCTTCCCACTCTGGACATCTCTGGTGGCCCTCCTTCTCTACCCCTTGTCTCTTGTGCTGGTCTATGTTCTTGATTATGTCTTTGGTTGGTCATAGATCAGCTGGAGTTTTGAGTTATATTCAGGGGGTTTGTTCATTTAAATTTCCGGATGTTTTTGCACATACTCTCTGCTGAGGCAATGCTATATATAACTACCTACTGTTCTGCTCTCTTTTGTTTGCTTCATTCTTGTGTTGGTATGATGTAGCAAGTGAATGAAAATAATGAGCATAATTTGTTCACAAAGTTGATGGCATGAAATAATCAATAGGAATAAGTTCAAATATAGCTCATAAGACAACTTAGTATATTTCAATTGGCTAATATAAATATCTAATAAAAAAGTTAGAACATGGTGAACTC

mRNA sequence

AAAGAATTATTAAATTCTTTCATCTTCCTCACAGAGAGGATCGTCCTCTCAACGTTCTCACACGGAAACACGGTTGCAAAGCTGATCCCAACAGTTTCAGTCCCAATCCAACCAATCCTTCTTTTAAGCTACACCCACCACTTTCTTCCTTAAACCCACTTCTTCTCTCTCGCCGGACCTCCAGATTTTGCCTCAAAAGCCATGTCCACCACTCCCCTGCATCTCCTCCTCCTCCTTTTCCTCATCCTCTGTGCTCAATCCCACAGCTCCCGCCTCGCTTCCATTTCTTCCCTCACTTCTTCCTCCTCCTCTGATCTGGTCTCCGATGGCGTCAATGAAATGCAGGAATCCCCTTATCTGCATCTCAACACGCTTTCTTCATTCTCTGCACCCGAGGAGGAGTCTTGCGAGCAGTCTTATGGGTTTCTTCCCTGTACCACCACTGCTTTGGGGAATCTCTTTTTGATTATCGTTTATGGCTATTTGATGTTCTTGGCTGCTAAATACTTGTCTACCGGAAGCGAGCTATTGTTGGAGATCTTGGGACCTGGAATTGTGGGGGGATTGTTCCTTCCCGCGCTTGGTGCTCTTCCTGATGCTATGCTCATTCTCGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCTGTTGGAATGGGCCTCCTAGCTGGCTCAACTGTCATGCTTCTAACACTGATCTGGGGTACATGTGTGATTGTTGGGAAGTGTGACCTTCAGGATTCAGTTGCCATAGATTCACAGGATACAAAAGGGTTTAGCTTGACTGAATCTGGAGTCAGTACTGATATTTGGACCAGCTATGCTGCCAGGATTATGGTTATATCTGTTTTTCCATTTTTAATAGTTCAACTTCCACAAATGCTCAATTCAACATCAGGAAGGCACTTGGCAGTGTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATCTATTGTCTTTACCAGGTCTTCCAGCCATGGATTCAAAGGAGGAAACTTGCCTTTGTCAAGCACAAACATGTTATATTTGGACTTTTGAGACATTTAAAACAGCAAGCACTTGGAAGGCTCCTGACAGAAAATGGGGAGCCAGATAAAGAAATAATAGAGAAGTTATTTTCAAGAATTGATGAGAACAGGGATGGACTTCTTTCAGCTTCTGAACTGAGAGCACTAATTGTTGGCATTCAATTTGAGGAGATCGACTTAGATCATGATGATGCTGTTGTGAAAATACTGAATGACTTCGATACTTCTCGTGATTCCCATGTTGATGCTACTGAGTTTGGCAATGGGATTATTAGGTGGCTTTCTCAAGTACAAGGTTCAAGAACTGGCCGAGGTGATGATGGTCCTCACACGATGAAGTTCCTGCATAACTTTCATCAAGAAACAAAGAGAGAGCATGATTTGCTGGATGTCGGGGAACAGAGTGATGAGGTCGTAGAGGGCGTTGAGGGTGGCAAGGGGGTCTTGATCAAAGCTATACTCTTCTTGTTAATTGGAACTGCCATTGCTGCTGCCTTTGCTGACCCTTTGGTCGACGTCGTGCATAACTTCTCCAATGCAACTAGCATCCCTGCGTTCTTTATTTCATTTATCGCTCTGCCATTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACTGCCTCGTTGACATTTTCTGAGCTATATGGAGCAGTGACAATGAACAATGTCCTCTGCCTATCAGTCTTCTTGGCTCTTGTTTACGTGAGAGGATTGGTTTGGAACTTTTCATCTGAAGTGCTGGTCATTCTCGTCGTCACGATTATAATGGGAGTTCTCGGTAGCTTTCGAACCGCCTTCCCACTCTGGACATCTCTGGTGGCCCTCCTTCTCTACCCCTTGTCTCTTGTGCTGGTCTATGTTCTTGATTATGTCTTTGGTTGGTCATAGATCAGCTGGAGTTTTGAGTTATATTCAGGGGGTTTGTTCATTTAAATTTCCGGATGTTTTTGCACATACTCTCTGCTGAGGCAATGCTATATATAACTACCTACTGTTCTGCTCTCTTTTGTTTGCTTCATTCTTGTGTTGGTATGATGTAGCAAGTGAATGAAAATAATGAGCATAATTTGTTCACAAAGTTGATGGCATGAAATAATCAATAGGAATAAGTTCAAATATAGCTCATAAGACAACTTAGTATATTTCAATTGGCTAATATAAATATCTAATAAAAAAGTTAGAACATGGTGAACTC

Coding sequence (CDS)

ATGTCCACCACTCCCCTGCATCTCCTCCTCCTCCTTTTCCTCATCCTCTGTGCTCAATCCCACAGCTCCCGCCTCGCTTCCATTTCTTCCCTCACTTCTTCCTCCTCCTCTGATCTGGTCTCCGATGGCGTCAATGAAATGCAGGAATCCCCTTATCTGCATCTCAACACGCTTTCTTCATTCTCTGCACCCGAGGAGGAGTCTTGCGAGCAGTCTTATGGGTTTCTTCCCTGTACCACCACTGCTTTGGGGAATCTCTTTTTGATTATCGTTTATGGCTATTTGATGTTCTTGGCTGCTAAATACTTGTCTACCGGAAGCGAGCTATTGTTGGAGATCTTGGGACCTGGAATTGTGGGGGGATTGTTCCTTCCCGCGCTTGGTGCTCTTCCTGATGCTATGCTCATTCTCGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCTGTTGGAATGGGCCTCCTAGCTGGCTCAACTGTCATGCTTCTAACACTGATCTGGGGTACATGTGTGATTGTTGGGAAGTGTGACCTTCAGGATTCAGTTGCCATAGATTCACAGGATACAAAAGGGTTTAGCTTGACTGAATCTGGAGTCAGTACTGATATTTGGACCAGCTATGCTGCCAGGATTATGGTTATATCTGTTTTTCCATTTTTAATAGTTCAACTTCCACAAATGCTCAATTCAACATCAGGAAGGCACTTGGCAGTGTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATCTATTGTCTTTACCAGGTCTTCCAGCCATGGATTCAAAGGAGGAAACTTGCCTTTGTCAAGCACAAACATGTTATATTTGGACTTTTGAGACATTTAAAACAGCAAGCACTTGGAAGGCTCCTGACAGAAAATGGGGAGCCAGATAAAGAAATAATAGAGAAGTTATTTTCAAGAATTGATGAGAACAGGGATGGACTTCTTTCAGCTTCTGAACTGAGAGCACTAATTGTTGGCATTCAATTTGAGGAGATCGACTTAGATCATGATGATGCTGTTGTGAAAATACTGAATGACTTCGATACTTCTCGTGATTCCCATGTTGATGCTACTGAGTTTGGCAATGGGATTATTAGGTGGCTTTCTCAAGTACAAGGTTCAAGAACTGGCCGAGGTGATGATGGTCCTCACACGATGAAGTTCCTGCATAACTTTCATCAAGAAACAAAGAGAGAGCATGATTTGCTGGATGTCGGGGAACAGAGTGATGAGGTCGTAGAGGGCGTTGAGGGTGGCAAGGGGGTCTTGATCAAAGCTATACTCTTCTTGTTAATTGGAACTGCCATTGCTGCTGCCTTTGCTGACCCTTTGGTCGACGTCGTGCATAACTTCTCCAATGCAACTAGCATCCCTGCGTTCTTTATTTCATTTATCGCTCTGCCATTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACTGCCTCGTTGACATTTTCTGAGCTATATGGAGCAGTGACAATGAACAATGTCCTCTGCCTATCAGTCTTCTTGGCTCTTGTTTACGTGAGAGGATTGGTTTGGAACTTTTCATCTGAAGTGCTGGTCATTCTCGTCGTCACGATTATAATGGGAGTTCTCGGTAGCTTTCGAACCGCCTTCCCACTCTGGACATCTCTGGTGGCCCTCCTTCTCTACCCCTTGTCTCTTGTGCTGGTCTATGTTCTTGATTATGTCTTTGGTTGGTCATAG

Protein sequence

MSTTPLHLLLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS
Homology
BLAST of Lsi04G020310 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 1.3e-201
Identity = 388/581 (66.78%), Postives = 453/581 (77.97%), Query Frame = 0

Query: 8   LLLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEE 67
           L+ LLFL+    S  +R  S++  ++S  SD + DG + +  S  +  + +S+ +  +EE
Sbjct: 6   LISLLFLLFFTSSAYARFVSLNPSSTSLISDGI-DGGSNLAGSGSVIKSVVSAPAEEKEE 65

Query: 68  SCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 127
           +CEQ+YGF+PCT TALGN+FLI+VYG+LMF AA YLS GSELLLEILGPGIVGGLFLP L
Sbjct: 66  ACEQTYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPML 125

Query: 128 GALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA 187
           GALPDAMLI+     G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL+DS+A
Sbjct: 126 GALPDAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIA 185

Query: 188 IDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIALI 247
           +++QDTKGF L +SGV+ DIWTSYAARIM ISV PF+IVQLPQML STSGR L+VLIALI
Sbjct: 186 VNNQDTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALI 245

Query: 248 ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEII 307
           +SV M I YC+YQVFQPWIQRR+LAF KHKHVI G+LRHLKQ ALGRLL + G+PD+ +I
Sbjct: 246 LSVLMLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVI 305

Query: 308 EKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRDSHVDATEF 367
            KLF  ID N DG LSA+EL+ALI+GI FE+ID D DDAV K+L DFD + D  VD  EF
Sbjct: 306 RKLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEF 365

Query: 368 GNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEG--VEG 427
             GI +WL Q  G      + GP TMKFL NFH +TKREH LL   E  +   EG  V  
Sbjct: 366 VRGIKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVAD 425

Query: 428 GKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 487
            K + IKA L LL+G AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVS
Sbjct: 426 PKWITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVS 485

Query: 488 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVILVVTI 547
           AIIFASR K +TASLTFSEL G VTMNN+LCLSVFLA+VYVRGL WNFSSEVLVIL+V +
Sbjct: 486 AIIFASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCL 545

Query: 548 IMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           +MG   SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 546 VMGGFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of Lsi04G020310 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 4.2e-184
Identity = 358/585 (61.20%), Postives = 449/585 (76.75%), Query Frame = 0

Query: 9   LLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPE--- 68
           L+ L L L A +   RL S  S  S+S++ L+S+ V     S  + L T +S SA     
Sbjct: 10  LVPLLLFLLAAAAYGRLISDGSPASASATSLLSNPV-----SAVIRLTTSNSASASSPPA 69

Query: 69  ---EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGL 128
              EE CEQSYGFLPCTTT LGNLFL++ YG+LM+ AA +LS GSELLLEI+GPG+VGGL
Sbjct: 70  AAPEEKCEQSYGFLPCTTTVLGNLFLVLAYGFLMYKAATFLSAGSELLLEIMGPGLVGGL 129

Query: 129 FLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL 188
            LP LGALPDA+L+L     GS E AQSQV +GMGLLAGSTV LLTL+WGTCV+VGKCD+
Sbjct: 130 LLPILGALPDALLVLVSGLSGSRETAQSQVLIGMGLLAGSTVFLLTLLWGTCVVVGKCDI 189

Query: 189 -QDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLA 248
             + VA+D Q+ KGFSLT +G+STD+ TSYAARIM ISV PF+I Q P+ML +  G+ LA
Sbjct: 190 GPNGVAVDLQNNKGFSLTGTGISTDVQTSYAARIMGISVIPFIIAQFPKMLKTHHGQRLA 249

Query: 249 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGE 308
           VL+ALI+S S+ + YCLYQVFQPWIQ+RKLA+ KHKHVI G+LRH + +ALGRLL E+G 
Sbjct: 250 VLLALIVSFSLVLAYCLYQVFQPWIQKRKLAYAKHKHVISGILRHAQMEALGRLLNEDGT 309

Query: 309 PDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRDSH 368
           P++++I+KLF +ID +    LS +EL ALI+GI FEE+D D +DAV KI++DFDTS +  
Sbjct: 310 PNEDVIKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDI 369

Query: 369 VDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVE 428
           V+  EF +G+ RWL++ + S       G ++ KF+ ++H  T++EHDLL   ++SDE VE
Sbjct: 370 VEEAEFVSGMKRWLNEAKRSVP---TSGAYSNKFITDYHARTRQEHDLL--VDRSDETVE 429

Query: 429 GVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 488
            VE     + KA+  LL+G+AIAAAFADPLVD VHNFSNA+ IP+FFISFIALPLATNSS
Sbjct: 430 SVENPGWCITKAVGLLLLGSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLATNSS 489

Query: 489 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVIL 548
           EAVSAIIFASR K +T+SLTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEVL+IL
Sbjct: 490 EAVSAIIFASRKKLRTSSLTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEVLIIL 549

Query: 549 VVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           +V +IMG+  SFRT FPLWT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 550 LVCVIMGLFTSFRTTFPLWTCLVAYMLYPLSLVVVYILDFVFGWS 584

BLAST of Lsi04G020310 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 612.1 bits (1577), Expect = 6.6e-174
Identity = 348/588 (59.18%), Postives = 436/588 (74.15%), Query Frame = 0

Query: 3   TTPLHLLLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDG--VNEMQESPYLHLNTLSS 62
           T  L +LLLL L+            ++ L +      +SDG   N    +  L L + ++
Sbjct: 8   TRCLPVLLLLLLV------------VAPLLAHGRRPFISDGGNANANANASVLRLPSAAA 67

Query: 63  FSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVG 122
            +A E+  CE SYGFLPCTTTA GNLFL++ YG+LMF +A YLS+GSE+LL+ILGPGIVG
Sbjct: 68  -AAGEDMGCEMSYGFLPCTTTAWGNLFLVLAYGFLMFKSATYLSSGSEMLLQILGPGIVG 127

Query: 123 GLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC 182
           GLFLP LGALPDA+LIL     G+ EVAQSQV +GMGLLAGSTVMLLTL+WG+CV+VGKC
Sbjct: 128 GLFLPILGALPDALLILVSGLSGTKEVAQSQVLIGMGLLAGSTVMLLTLLWGSCVVVGKC 187

Query: 183 DL-QDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 242
           DL ++S AIDS+DTKGFSL  SGVSTD  TSYAARIM IS+ PF+IVQ+P++    SG  
Sbjct: 188 DLSENSTAIDSRDTKGFSLLGSGVSTDKQTSYAARIMAISILPFIIVQIPKIFKLHSGHQ 247

Query: 243 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 302
           + VLI LI++  + + YCLYQVFQPWIQRR+L + + KHV+ GLLRH ++ ++GRLL + 
Sbjct: 248 ITVLIGLIVAALLLLSYCLYQVFQPWIQRRRLEYTRLKHVMSGLLRHAQKHSIGRLLDDE 307

Query: 303 GEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRD 362
           G P+  +IEKLF RID++ DG L   EL+A IVGI FE+ID + + A  +++ DFDTSR+
Sbjct: 308 GRPNVSVIEKLFHRIDQDNDGKLERGELQAFIVGINFEDIDWNSNLAADQVMADFDTSRN 367

Query: 363 SHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREH-DLLDVGEQSDE 422
             ++  EF NG++RWL + + + T     G ++ KFL++FH  T+ E   LLD  E+  E
Sbjct: 368 HFIEKGEFVNGMLRWLDEAKRTVT----SGAYSKKFLNDFHARTRDEQTGLLDKDEEEGE 427

Query: 423 VVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLAT 482
             +G        IKAIL LL+GTA+AAA ADPLVD VHNFSNAT IP+FFISFI +PLAT
Sbjct: 428 -ADG--NPTWTCIKAILLLLLGTAMAAASADPLVDAVHNFSNATHIPSFFISFIVMPLAT 487

Query: 483 NSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVL 542
           NSSEAVSAIIFASR K++T SLTFSE+YG VTMNN LCL+VFLALVYVRGL W+FSSEVL
Sbjct: 488 NSSEAVSAIIFASRKKKRTLSLTFSEVYGGVTMNNTLCLAVFLALVYVRGLTWDFSSEVL 547

Query: 543 VILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           +IL+V IIMG+  SFRT FPLWT  VA LLYPLSL++VY+LDY FGWS
Sbjct: 548 IILLVCIIMGLFTSFRTDFPLWTCFVAFLLYPLSLIMVYILDYKFGWS 575

BLAST of Lsi04G020310 vs. ExPASy TrEMBL
Match: E5GBG6 (Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.2e-296
Identity = 550/585 (94.02%), Postives = 566/585 (96.75%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT---SSSSSDLVSDGVNEMQESPYLHLN 60
           MS +PLHLL LL FL+LC+QS+SSRL SISSLT   SSSSSDLVSDGVNE+QE  YLHLN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120
           TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           GIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 GIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLA 240
           LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRHLA
Sbjct: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLA 240

Query: 241 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGE 300
           VLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLTENGE
Sbjct: 241 VLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGE 300

Query: 301 PDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRDSH 360
           PDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+EIDLDHDDAV KI+NDFDTSRDSH
Sbjct: 301 PDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSH 360

Query: 361 VDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVE 420
           VD+ EFGNGIIRWLSQVQGSRTGRGDDGPHTMK+LHNFHQETKREHDLLDVGEQSDEVVE
Sbjct: 361 VDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVE 420

Query: 421 GVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 480
           GVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSS
Sbjct: 421 GVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVIL 540

Query: 541 VVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           VVT++MGV+GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 585

BLAST of Lsi04G020310 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 3.0e-294
Identity = 550/590 (93.22%), Postives = 566/590 (95.93%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT---SSSSSDLVSDGVNEMQESPYLHLN 60
           MS +PLHLL LL FL+LC+QS+SSRL SISSLT   SSSSSDLVSDGVNE+QE  YLHLN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120
           TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180
           GIVGGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTS 240
           VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 241 GRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLL 300
           GRHLAVLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 301 TENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDT 360
           TENGEPDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+EIDLDHDDAV KI+NDFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 361 SRDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQS 420
           SRDSHVD+ EFGNGIIRWLSQVQGSRTGRGDDGPHTMK+LHNFHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 421 DEVVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPL 480
           DEVVEGVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSE 540
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 541 VLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           VLVILVVT++MGV+GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of Lsi04G020310 vs. ExPASy TrEMBL
Match: A0A1S3CJX3 (uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=4 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 3.0e-294
Identity = 550/590 (93.22%), Postives = 566/590 (95.93%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT---SSSSSDLVSDGVNEMQESPYLHLN 60
           MS +PLHLL LL FL+LC+QS+SSRL SISSLT   SSSSSDLVSDGVNE+QE  YLHLN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120
           TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180
           GIVGGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTS 240
           VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 241 GRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLL 300
           GRHLAVLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 301 TENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDT 360
           TENGEPDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+EIDLDHDDAV KI+NDFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 361 SRDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQS 420
           SRDSHVD+ EFGNGIIRWLSQVQGSRTGRGDDGPHTMK+LHNFHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 421 DEVVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPL 480
           DEVVEGVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSE 540
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 541 VLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           VLVILVVT++MGV+GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of Lsi04G020310 vs. ExPASy TrEMBL
Match: A0A0A0L3B8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G628350 PE=4 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.8e-292
Identity = 546/589 (92.70%), Postives = 565/589 (95.93%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT--SSSSSDLVSDGVNEMQESPYLHLNT 60
           MS +PLHLL LL FL+LC+QS+SSRL SISS+T  SSSSSDLVSDG+N +QE  YLHLNT
Sbjct: 1   MSNSPLHLLFLLFFLVLCSQSYSSRLPSISSITSSSSSSSDLVSDGINGLQEPSYLHLNT 60

Query: 61  LSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPG 120
           LSS SAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAA YLSTGSELLLEILGPG
Sbjct: 61  LSSLSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG 120

Query: 121 IVGGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180
           IVGGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV
Sbjct: 121 IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180

Query: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSG 240
           GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSG
Sbjct: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSG 240

Query: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLT 300
           RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLT
Sbjct: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLT 300

Query: 301 ENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTS 360
           ENGEPDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+E+DLDHDDAV KI+NDFDTS
Sbjct: 301 ENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTS 360

Query: 361 RDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSD 420
           RDSHVD+ EFGNGIIRWLSQVQGSRTGRG+DGPHTMK+LHNFHQETKREHDLLDVGEQSD
Sbjct: 361 RDSHVDSNEFGNGIIRWLSQVQGSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSD 420

Query: 421 EVVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLA 480
           EVVEGVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLA
Sbjct: 421 EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLA 480

Query: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEV 540
           TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEV
Sbjct: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEV 540

Query: 541 LVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           LVIL+VT+IMGV+GSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 LVILIVTMIMGVMGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 589

BLAST of Lsi04G020310 vs. ExPASy TrEMBL
Match: A0A6J1IGZ1 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE=4 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 9.3e-288
Identity = 541/587 (92.16%), Postives = 559/587 (95.23%), Query Frame = 0

Query: 1   MSTTPLHLLLLL-FLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLS 60
           MS TPLHL+ LL FLILC QSHSSR ASI     SSSSDLVSDGVNE+QE  YL LNTLS
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASI-----SSSSDLVSDGVNELQEPSYLRLNTLS 60

Query: 61  SFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIV 120
           S SAP EESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIV
Sbjct: 61  SNSAP-EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIV 120

Query: 121 GGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180
           GGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK
Sbjct: 121 GGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180

Query: 181 CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 240
           CDL+ SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRH
Sbjct: 181 CDLEGSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRH 240

Query: 241 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 300
           LAVLIALIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN
Sbjct: 241 LAVLIALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 300

Query: 301 GEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRD 360
           GEPDKEII+KLFSRIDEN+DG LSASELRALIVGIQFEE+DLDH+DAV KILNDFDTSRD
Sbjct: 301 GEPDKEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRD 360

Query: 361 SHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV 420
           SHVDATEFGNGII+WLSQVQGSRTGR DDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV
Sbjct: 361 SHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV 420

Query: 421 VEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 480
           VE VEGGKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATN
Sbjct: 421 VEDVEGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATN 480

Query: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLV 540
           SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLV
Sbjct: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLV 540

Query: 541 ILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           ILVVT+IMG+LGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 ILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Lsi04G020310 vs. NCBI nr
Match: XP_038897650.1 (sodium/calcium exchanger NCL [Benincasa hispida])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.7e-299
Identity = 559/587 (95.23%), Postives = 573/587 (97.61%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLS 60
           MS++PLHLL  L FLILC+QSHSSRLASISSLTSSSSSDLVSDGVNE+QE PYLHLNTLS
Sbjct: 1   MSSSPLHLLFFLFFLILCSQSHSSRLASISSLTSSSSSDLVSDGVNELQEPPYLHLNTLS 60

Query: 61  SFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIV 120
           SFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIV
Sbjct: 61  SFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIV 120

Query: 121 GGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180
           GGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK
Sbjct: 121 GGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180

Query: 181 CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 240
           CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH
Sbjct: 181 CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 240

Query: 241 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 300
           LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQ+LGRLL EN
Sbjct: 241 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQSLGRLLREN 300

Query: 301 GEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRD 360
           GEPDKEII+KLFS IDEN+DGLLSASELRALIVGIQFEEIDLDHDDAV KI+NDFDTSRD
Sbjct: 301 GEPDKEIIDKLFSSIDENKDGLLSASELRALIVGIQFEEIDLDHDDAVNKIMNDFDTSRD 360

Query: 361 SHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV 420
           SHV+ATEFGNGIIRWL+QVQGSR G+GDDGPHTMK+LHNFHQETKREHDLLDVGEQSDEV
Sbjct: 361 SHVNATEFGNGIIRWLTQVQGSRPGKGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEV 420

Query: 421 VEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 480
           VEGVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN
Sbjct: 421 VEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 480

Query: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLV 540
           SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLV
Sbjct: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLV 540

Query: 541 ILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           ILVVTIIMGVLGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 ILVVTIIMGVLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 587

BLAST of Lsi04G020310 vs. NCBI nr
Match: ADN33808.1 (sodium/calcium exchanger family protein [Cucumis melo subsp. melo])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.6e-296
Identity = 550/585 (94.02%), Postives = 566/585 (96.75%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT---SSSSSDLVSDGVNEMQESPYLHLN 60
           MS +PLHLL LL FL+LC+QS+SSRL SISSLT   SSSSSDLVSDGVNE+QE  YLHLN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120
           TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           GIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 GIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLA 240
           LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRHLA
Sbjct: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLA 240

Query: 241 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGE 300
           VLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLTENGE
Sbjct: 241 VLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGE 300

Query: 301 PDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRDSH 360
           PDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+EIDLDHDDAV KI+NDFDTSRDSH
Sbjct: 301 PDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSH 360

Query: 361 VDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVE 420
           VD+ EFGNGIIRWLSQVQGSRTGRGDDGPHTMK+LHNFHQETKREHDLLDVGEQSDEVVE
Sbjct: 361 VDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVE 420

Query: 421 GVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 480
           GVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSS
Sbjct: 421 GVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVIL 540

Query: 541 VVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           VVT++MGV+GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 585

BLAST of Lsi04G020310 vs. NCBI nr
Match: XP_008463718.1 (PREDICTED: uncharacterized protein LOC103501799 [Cucumis melo] >KAA0066783.1 sodium/calcium exchanger family protein [Cucumis melo var. makuwa] >TYK27930.1 sodium/calcium exchanger family protein [Cucumis melo var. makuwa])

HSP 1 Score: 1020.4 bits (2637), Expect = 6.2e-294
Identity = 550/590 (93.22%), Postives = 566/590 (95.93%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT---SSSSSDLVSDGVNEMQESPYLHLN 60
           MS +PLHLL LL FL+LC+QS+SSRL SISSLT   SSSSSDLVSDGVNE+QE  YLHLN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120
           TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180
           GIVGGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTS 240
           VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 241 GRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLL 300
           GRHLAVLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 301 TENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDT 360
           TENGEPDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+EIDLDHDDAV KI+NDFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 361 SRDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQS 420
           SRDSHVD+ EFGNGIIRWLSQVQGSRTGRGDDGPHTMK+LHNFHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 421 DEVVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPL 480
           DEVVEGVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSE 540
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 541 VLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           VLVILVVT++MGV+GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of Lsi04G020310 vs. NCBI nr
Match: XP_004146032.1 (sodium/calcium exchanger NCL [Cucumis sativus] >KGN55087.1 hypothetical protein Csa_012240 [Cucumis sativus])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.9e-292
Identity = 546/589 (92.70%), Postives = 565/589 (95.93%), Query Frame = 0

Query: 1   MSTTPLHLL-LLLFLILCAQSHSSRLASISSLT--SSSSSDLVSDGVNEMQESPYLHLNT 60
           MS +PLHLL LL FL+LC+QS+SSRL SISS+T  SSSSSDLVSDG+N +QE  YLHLNT
Sbjct: 1   MSNSPLHLLFLLFFLVLCSQSYSSRLPSISSITSSSSSSSDLVSDGINGLQEPSYLHLNT 60

Query: 61  LSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPG 120
           LSS SAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAA YLSTGSELLLEILGPG
Sbjct: 61  LSSLSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG 120

Query: 121 IVGGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180
           IVGGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV
Sbjct: 121 IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180

Query: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSG 240
           GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSG
Sbjct: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSG 240

Query: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLT 300
           RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLT
Sbjct: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLT 300

Query: 301 ENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTS 360
           ENGEPDKEIIEKLFSRID N+DGLLSASELRALIVGIQF+E+DLDHDDAV KI+NDFDTS
Sbjct: 301 ENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTS 360

Query: 361 RDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSD 420
           RDSHVD+ EFGNGIIRWLSQVQGSRTGRG+DGPHTMK+LHNFHQETKREHDLLDVGEQSD
Sbjct: 361 RDSHVDSNEFGNGIIRWLSQVQGSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSD 420

Query: 421 EVVEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLA 480
           EVVEGVE GKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLA
Sbjct: 421 EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLA 480

Query: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEV 540
           TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEV
Sbjct: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEV 540

Query: 541 LVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           LVIL+VT+IMGV+GSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 LVILIVTMIMGVMGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 589

BLAST of Lsi04G020310 vs. NCBI nr
Match: XP_023536443.1 (sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1002.3 bits (2590), Expect = 1.7e-288
Identity = 543/587 (92.50%), Postives = 561/587 (95.57%), Query Frame = 0

Query: 1   MSTTPLHLLLLL-FLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLS 60
           MS TPLHL+ LL FLILC QSHSSR ASI    SSSSSDLVSDGVN++QE  YL LNTLS
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASI----SSSSSDLVSDGVNDLQEPSYLRLNTLS 60

Query: 61  SFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIV 120
           S SAP EESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIV
Sbjct: 61  SNSAP-EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIV 120

Query: 121 GGLFLPALGALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180
           GGLFLPALGALPDAMLIL     GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK
Sbjct: 121 GGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180

Query: 181 CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 240
           CDL+ SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH
Sbjct: 181 CDLEGSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 240

Query: 241 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 300
           LAVLIALIIS+SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN
Sbjct: 241 LAVLIALIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 300

Query: 301 GEPDKEIIEKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRD 360
           GEPDKEII+KLFSRIDEN+DG LSASELRALIVGIQFEE+DLDH+DAV KILNDFDTSRD
Sbjct: 301 GEPDKEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRD 360

Query: 361 SHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV 420
           SHVDATEFGNGII+WLSQVQGSRTGR DDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV
Sbjct: 361 SHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEV 420

Query: 421 VEGVEGGKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 480
           VE VEGGKGVLIKAILFLL+GTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATN
Sbjct: 421 VEDVEGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATN 480

Query: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLV 540
           SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLV
Sbjct: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLV 540

Query: 541 ILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           ILVVT+IMG+LGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 ILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 582

BLAST of Lsi04G020310 vs. TAIR 10
Match: AT1G53210.1 (sodium/calcium exchanger family protein / calcium-binding EF hand family protein )

HSP 1 Score: 704.1 bits (1816), Expect = 9.1e-203
Identity = 388/581 (66.78%), Postives = 453/581 (77.97%), Query Frame = 0

Query: 8   LLLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEE 67
           L+ LLFL+    S  +R  S++  ++S  SD + DG + +  S  +  + +S+ +  +EE
Sbjct: 6   LISLLFLLFFTSSAYARFVSLNPSSTSLISDGI-DGGSNLAGSGSVIKSVVSAPAEEKEE 65

Query: 68  SCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 127
           +CEQ+YGF+PCT TALGN+FLI+VYG+LMF AA YLS GSELLLEILGPGIVGGLFLP L
Sbjct: 66  ACEQTYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPML 125

Query: 128 GALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA 187
           GALPDAMLI+     G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL+DS+A
Sbjct: 126 GALPDAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIA 185

Query: 188 IDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIALI 247
           +++QDTKGF L +SGV+ DIWTSYAARIM ISV PF+IVQLPQML STSGR L+VLIALI
Sbjct: 186 VNNQDTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALI 245

Query: 248 ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEII 307
           +SV M I YC+YQVFQPWIQRR+LAF KHKHVI G+LRHLKQ ALGRLL + G+PD+ +I
Sbjct: 246 LSVLMLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVI 305

Query: 308 EKLFSRIDENRDGLLSASELRALIVGIQFEEIDLDHDDAVVKILNDFDTSRDSHVDATEF 367
            KLF  ID N DG LSA+EL+ALI+GI FE+ID D DDAV K+L DFD + D  VD  EF
Sbjct: 306 RKLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEF 365

Query: 368 GNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEG--VEG 427
             GI +WL Q  G      + GP TMKFL NFH +TKREH LL   E  +   EG  V  
Sbjct: 366 VRGIKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVAD 425

Query: 428 GKGVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 487
            K + IKA L LL+G AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVS
Sbjct: 426 PKWITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVS 485

Query: 488 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVILVVTI 547
           AIIFASR K +TASLTFSEL G VTMNN+LCLSVFLA+VYVRGL WNFSSEVLVIL+V +
Sbjct: 486 AIIFASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCL 545

Query: 548 IMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 582
           +MG   SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 546 VMGGFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of Lsi04G020310 vs. TAIR 10
Match: AT1G29025.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 233.0 bits (593), Expect = 6.0e-61
Identity = 187/593 (31.53%), Postives = 301/593 (50.76%), Query Frame = 0

Query: 9   LLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEES 68
           L  L L L +  +   L+S+S    ++SS LVSDGV +   + +L LN  +      E +
Sbjct: 8   LSFLSLALVSVVNCRVLSSVSE--DNNSSILVSDGVQDRSSNDFLSLNPPNL----SESA 67

Query: 69  CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALG 128
           C   YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+  P L 
Sbjct: 68  CVHVYGFLPCADNIEGYVFQVFSFGCLLIIGEYFLSKGRSKLFVIFEVGFFGGIIFPLLT 127

Query: 129 ALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ---DS 188
             P   LIL     GS E+A S     + +  G +V  LT+ WG CV+ G   L+    S
Sbjct: 128 MFPRIALILSTGLIGSREIANSMTGNNVAVTVGYSVFALTMQWGACVVFGLSGLRSDDQS 187

Query: 189 VAID-----SQDTKGFSLTE----SGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTS 248
           +  D      Q  K  +L +    + +  D     AA IM++++ PF++V   +  +  S
Sbjct: 188 ITCDISSPRRQVKKSINLLKNLAGASIRADPKNKKAAGIMLLTLLPFVLVTFSETFHIKS 247

Query: 249 GRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVK-------HKHVIFGLLRHLKQ 308
                VL+ LI S S  ++Y +Y  F    Q + L + K       HKH     L++   
Sbjct: 248 WDDNMVLVTLIFSGSATVLYFVYLYFDKADQVKSLEYAKFELMSEVHKH-----LQNFSP 307

Query: 309 QALGRLLTENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGI-QFEEIDLDHDDAVV 368
           Q+L R    NG+  +E ++ LF + D N+DG +  SEL+ L V       +  D ++   
Sbjct: 308 QSLIR----NGQLCQESLKSLFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDINELAT 367

Query: 369 KILNDFDTSRDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHD 428
            +L DFD++RD  +D TEF  GI +WL Q + S  G    G           ++   +  
Sbjct: 368 TLLADFDSNRDGEIDETEFAIGIEKWLKQYKFSFNGTESQG-----------EDIAEDDG 427

Query: 429 LLDVGEQSDEVVEGVEGGKGV---LIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIP 488
           +L V    +++++G    K +    +KA++ ++IG  I +  A P +  +   S +  +P
Sbjct: 428 VLKV----EQLLKGCLFTKLLTKRTLKAVIEVIIGITIVSFLAMPFMMNIELLSVSAGVP 487

Query: 489 AFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY 548
           +F++ F  +PLA N   A+SA     ++K K  S TFSE+Y  VT+NN+L +++ LA+VY
Sbjct: 488 SFYVVFAVIPLARNLKNALSAHFCRKKEKAKITSDTFSEIYRDVTLNNLLGITIILAIVY 547

Query: 549 VRGLVWNFSSEVLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYV 574
           +RGL W++S+EVL+I++V +I+GV    R+ +P W  ++A  LY  SL+L+Y+
Sbjct: 548 IRGLTWDYSTEVLIIVIVGLIIGVPAYVRSTYPFWICVLAFALYFFSLILIYL 570

BLAST of Lsi04G020310 vs. TAIR 10
Match: AT2G34030.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 222.6 bits (566), Expect = 8.1e-58
Identity = 180/582 (30.93%), Postives = 288/582 (49.48%), Query Frame = 0

Query: 11  LLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEESCE 70
           LL + L     SSR+  + S    ++S L+SDG+++     +L L+   S S     +C 
Sbjct: 10  LLSIALIISGVSSRV--LISHVPLNNSILISDGIHDALNHEFLTLDPPKSLS---RTACV 69

Query: 71  QSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGAL 130
             YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+  P L   
Sbjct: 70  HVYGFLPCADNVEGYIFQVFSFGSLLIIGDYFLSEGRSKLFVIFEVGFYGGIIFPLLTMF 129

Query: 131 PDAMLILG-----SAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG----KCDLQDSV 190
           P   L+L      S +VA S +   +GL  G TV  LT+ WG CV+      + D  D +
Sbjct: 130 PRIALMLSTGLSLSRDVASSFIDDNVGLTVGHTVFSLTIQWGACVVFSITGPRSDQADGL 189

Query: 191 AIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIAL 250
               +  KGF   E+ V  D     AA IM++S+ PFL+V    + +S S  H+ VLI L
Sbjct: 190 IEKLKILKGF--VEARVEADPKNKKAAGIMLLSLSPFLMVTFSAIFDSHSWSHIIVLITL 249

Query: 251 IISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEI 310
           IIS S  ++Y +Y       Q + L   + + ++  + +HLK+ +   L+ ++GE  KE 
Sbjct: 250 IISSSSTVVYFVYSYLDTANQEKSLDHARFE-LMSEVHKHLKRFSPKHLI-KDGELSKES 309

Query: 311 IEKLFSRIDENRDGLLSASELRALIVGI-QFEEIDLDHDDAVVKILNDFDTSRDSHVDAT 370
           ++ LF + D+N+DG +  SEL+ L + +  F  +  D ++     L DFD   D  ++  
Sbjct: 310 LKSLFKKTDKNKDGKIQISELKDLTIELSNFGRMRYDINELAKAFLEDFDGDNDGELEEN 369

Query: 371 EFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEGVEG 430
           EF  GI R L Q                   + F+ E +RE+   + G    E+      
Sbjct: 370 EFEEGIARLLKQ-------------------YKFNVEDQRENQTEENGVLKLEIKP---- 429

Query: 431 GKGVLIKAILFL---------LIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPL 490
            K  L+  +L +         ++G  I    A P +  +   S +  IP+F+I F  +P 
Sbjct: 430 -KKTLVTKLLSMETLIATTEVIVGILIVLFLAKPFMLNIQLLSISAGIPSFYIVFAMIPF 489

Query: 491 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSE 550
           A N    +S      +DK++ +S TFSE+Y  +TMNN+L +SV LA+VY RGL W +S E
Sbjct: 490 ARNLKNTLSTRFCRGKDKKRVSSNTFSEIYKDITMNNLLGMSVILAIVYTRGLTWKYSVE 549

Query: 551 VLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSLVLVYV 574
            L++++V +I+G+    R+ +P W  ++A  +Y  SL+L+Y+
Sbjct: 550 TLIMVIVGLIIGLPIYIRSTYPFWMCVLAFAMYFFSLLLIYI 558

BLAST of Lsi04G020310 vs. TAIR 10
Match: AT1G29020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 218.0 bits (554), Expect = 2.0e-56
Identity = 174/570 (30.53%), Postives = 285/570 (50.00%), Query Frame = 0

Query: 9   LLLLFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEES 68
           L L FL L A         +SSL S   S LVSDGV +     +L L+  +  +   +  
Sbjct: 6   LFLSFLSLIALISVVNCRVLSSL-SEKDSILVSDGVQDGSSYEFLSLDPPNDIT---KNM 65

Query: 69  CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALG 128
           C   Y FLPC     G +F +  +G ++ +   +L+ G   L  I   G  GG+  P L 
Sbjct: 66  CVHVYDFLPCADNVAGYVFQVFSFGCILIIGEYFLTKGRTKLFLIFEVGFYGGIIFPLLT 125

Query: 129 ALPDAMLIL-----GSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAI 188
             P   LIL     GS E+A S+V   +G+  G +V  LT+ WG C++ G    +    I
Sbjct: 126 MFPRIALILSTGLVGSREMASSRVGNIIGVTVGYSVFALTIQWGACILFGLSGTKPEQPI 185

Query: 189 DSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIALII 248
           D        L+ + V  DI    +A IM++++ PF++V   ++ +S     + VLI LII
Sbjct: 186 D--------LSAASVEADITNKKSAGIMLLTILPFILVTFSEISSSQYWNDIIVLITLII 245

Query: 249 SVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIE 308
           S S    Y  Y  F    Q + L   + + ++  + +HL Q    + L  +G+ ++E ++
Sbjct: 246 SASATFAYFFYSYFDRDDQAKSLDHARFE-LMSEVHKHL-QIFSPKSLIRDGQLNQESLK 305

Query: 309 KLFSRIDENRDGLLSASELRALIVGI-QFEEIDLDHDDAVVKILNDFDTSRDSHVDATEF 368
           +LF +ID N DG +  +EL+ L V       +    D  V  +++DFD   D  +D  EF
Sbjct: 306 RLFQKIDANGDGKIQVAELKDLTVEFGMIGRVKCHIDKLVHTLVDDFDRDGDREIDEAEF 365

Query: 369 GNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEGVEGGK 428
             GI +WL+Q + S                   ++      +L V +    +V+ +   +
Sbjct: 366 EIGIKKWLNQYKFSFDTTVPP-----------REDQAEGVPILKVEKPEQSLVKKLLSWR 425

Query: 429 GVLIKAILFLLIGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI 488
              ++A L +++G  +    A P +  +   S +  +P+F++ F+ +PLA N    +SA 
Sbjct: 426 --TLRASLEVILGMLVVIYLARPFMMNIQLLSESAGVPSFYVVFVVIPLARNLKNTLSAH 485

Query: 489 IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVILVVTIIM 548
               +DK K  S TFSE+Y  VTMNN++ +S+ LA+VY R LVW++++EVL+I++V +++
Sbjct: 486 FCRKKDKAKITSDTFSEIYKDVTMNNLMGISIILAIVYARELVWDYTTEVLIIVIVGLLI 545

Query: 549 GVLGSFRTAFPLWTSLVALLLYPLSLVLVY 573
           G+    RT +P W  L+A  LY +SLV+VY
Sbjct: 546 GLPAYVRTTYPFWICLLAFALYFISLVVVY 548

BLAST of Lsi04G020310 vs. TAIR 10
Match: AT2G34020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 211.8 bits (538), Expect = 1.4e-54
Identity = 188/613 (30.67%), Postives = 296/613 (48.29%), Query Frame = 0

Query: 12  LFLILCAQSHSSRLASISSLTSSSSSDLVSDGVNEMQESPYLHLNTLSSFSAPEEESCEQ 71
           L L L     SSR+ S   L +SS   L+SDGV+   +  YL L+   + S   + +C  
Sbjct: 24  LSLTLFISGVSSRVLSPVPLDNSS---LISDGVHGASDYKYLTLDPPKNVS---KAACIH 83

Query: 72  SYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGALP 131
            YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+  P L   P
Sbjct: 84  VYGFLPCADNIGGYVFQVFSFGCLLIIGDYFLSQGRSKLFVIFEVGFYGGIVFPLLTMFP 143

Query: 132 DAMLILGSAEVAQSQ-----VSVGMGLLAGSTVMLLTLIWGTCVIVGKCD-------LQD 191
              L++     A  +     V   +G+  G T+  LT+ WG CVI G           + 
Sbjct: 144 RIALMISPGLAATHEGALMIVGNNVGVTIGHTIFALTMQWGACVIFGLTSPNSDPSIRRG 203

Query: 192 SVAIDSQDTK----GF-------SLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLN 251
           S+   S DTK    GF       S+ E+ V  D     AA IM++++ PFL+V LP +L+
Sbjct: 204 SIKRTSSDTKNPRRGFYRMKILKSVVEASVDADPKNKKAAGIMLLTLVPFLLVTLPDLLD 263

Query: 252 STSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALG 311
             S   + +LI LIIS S   IY +Y  F    Q++ L   K + ++  + +HL Q    
Sbjct: 264 VQSWSDIIMLITLIISCSSTFIYFVYSYFDTADQKKSLDHAKFE-LMSEVHKHL-QSFSP 323

Query: 312 RLLTENGEPDKEIIEKLFSRIDENRDGLLSASELRALIVGI-QFEEIDLDHDDAVVKILN 371
           R L  +G+  KE ++ LF +ID N+DG +  SEL+ L V    F ++  D ++    +L 
Sbjct: 324 RTLIRDGQLSKESLKSLFDKIDRNKDGKIQISELKDLTVEFGVFGKMKCDINEFASTLLA 383

Query: 372 DFDTSRDSHVDATEFGNGIIRWLSQVQGSRTGRGDDGPHTMKFLHNFHQETKREHDLLDV 431
           +FD  ++  +D  EF  GI++ L+  +                    +QE+ R+++    
Sbjct: 384 EFDKDKNGELDENEFEEGIMKLLNHYKFD------------------NQESPRQNNTYIY 443

Query: 432 GEQSDEVVEGVEGGK-GVL------------------IKAILFLLIGTAIAAAFADPLVD 491
              SD V    +G + GVL                  ++A+  ++ G  I    A P + 
Sbjct: 444 RTPSDSVKSLSQGEEAGVLKLEMPKQTLVAKFLSMSTLRAVTKVIGGMLIVVFLAKPFMV 503

Query: 492 VVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNN 551
            +   S +  +P+F+  F  +PL  N    +SA     +DK + AS  FSE+Y  VTMNN
Sbjct: 504 NIGLLSVSAGVPSFYSVFAVIPLVRNLKNTLSAHFCRKKDKARIASEKFSEIYRDVTMNN 563

Query: 552 VLCLSVFLALVYVRGLVWNFSSEVLVILVVTIIMGVLGSFRTAFPLWTSLVALLLYPLSL 582
           ++ +S+ LA+VY RGL W +S E L+++VV I +G+    R+ +P W  ++A  +Y  SL
Sbjct: 564 LMGMSITLAIVYSRGLKWEYSIESLLVVVVGIAIGLPAYVRSTYPFWICVMAFAMYIFSL 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6361.3e-20166.78Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1[more]
Q5QNI24.2e-18461.20Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 P... [more]
Q6K3R56.6e-17459.18Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 P... [more]
Match NameE-valueIdentityDescription
E5GBG64.2e-29694.02Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE... [more]
A0A5A7VFE43.0e-29493.22Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CJX33.0e-29493.22uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=... [more]
A0A0A0L3B84.8e-29292.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G628350 PE=4 SV=1[more]
A0A6J1IGZ19.3e-28892.16sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE... [more]
Match NameE-valueIdentityDescription
XP_038897650.11.7e-29995.23sodium/calcium exchanger NCL [Benincasa hispida][more]
ADN33808.18.6e-29694.02sodium/calcium exchanger family protein [Cucumis melo subsp. melo][more]
XP_008463718.16.2e-29493.22PREDICTED: uncharacterized protein LOC103501799 [Cucumis melo] >KAA0066783.1 sod... [more]
XP_004146032.19.9e-29292.70sodium/calcium exchanger NCL [Cucumis sativus] >KGN55087.1 hypothetical protein ... [more]
XP_023536443.11.7e-28892.50sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G53210.19.1e-20366.78sodium/calcium exchanger family protein / calcium-binding EF hand family protein... [more]
AT1G29025.16.0e-6131.53Calcium-binding EF-hand family protein [more]
AT2G34030.18.1e-5830.93Calcium-binding EF-hand family protein [more]
AT1G29020.12.0e-5630.53Calcium-binding EF-hand family protein [more]
AT2G34020.11.4e-5430.67Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 302..325
e-value: 5.5E-5
score: 22.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 297..332
score: 12.281773
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 114..277
e-value: 2.8E-5
score: 25.6
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 429..572
e-value: 5.6E-11
score: 42.6
coord: 87..258
e-value: 1.9E-7
score: 31.1
NoneNo IPR availableGENE3D1.10.238.10coord: 278..401
e-value: 5.1E-9
score: 37.9
NoneNo IPR availablePANTHERPTHR31503:SF46SUBFAMILY NOT NAMEDcoord: 7..579
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 7..579
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 310..322
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 283..506

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G020310.1Lsi04G020310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity