Lsi04G014190 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G014190
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCytochrome P450, putative
Locationchr04: 21949301 .. 21952797 (-)
RNA-Seq ExpressionLsi04G014190
SyntenyLsi04G014190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATGGGATTGAGTTTTACTCTTCCACTAGAGGCTTCTTTTGCTGCTGAGTTTGTTTAATGTAATGGTCGAATTGAATTTTTGTGTTAAAAACATTATGAAAAAAGCGAAGTAGGCTGCAGAATATGATGAATTCTTCACCATCCAAACATTTCAACGATGCAGAATAACGAAAGAATAAAAGGACTTCAATGGTCGTTTCATTATTTCCACTCCAATAATCATTCGTGTACCGACAAAAGTACCTACCTTAGTGAAACTCCCACTGCCACCCCCTCCTTTCCATTTTCATTTCCTCACCATTTGCCTTATTTAAACACAAATGAAACACCACAACCAAATAAACTCTCAACAGTCAACGCAGTCCCTCCCTTTCCCTTTCTCCTTCTTCTCTCTCCCTTTTCCTCGGTTTGGGTATCGAGAAAAATGGATGATTTTCTTCAATCAGTGTTGGCGAGTGTGTCTATGGGAGTGGGTGCAATATTGTTCACGACGTTGTGCGTCGTGGGAGGTGGGTTGGTTTATTTCTATGGACCTTACTGGGGAGTGAGAAGGGTGCCTGGGCCACCAGCTATTCCGCTGGTCGGACACCTTCCCTTACTGGCCAAGTACGGCCCAGACGTTTTCTCTGTCCTCGCCTCCCAATATGGTCCTATCTTCAGGTCAGTTTCCTGTCTTCCTTTTTGACTTTCCCATTTCAAAACTCCTTCCTTTTTGCTTCCGAGTTCCGAGATGGTCTCCAAACGACGTCGCTTGTCTTGCCTTGGCTCGACTGTGCAATAATTTAGAGGGCTAGTGCAGGCATCATCTGCTCCTGTTCACTTGCACGCTCGAAACAGCTTTTCTTTTCGTTAAATTATGAATTTAAAAGGCTTAAGTATTTCTATATATCCCCTGCAAATTTCAAACTCGGTGGATGGCTGTATGATAGACAGGGGAATTTGATATCTATTGATGTGAAATGAAAAGGGAATAACAACGTGGCAATATTCAATGATTGTAATAATATGCTAGTGGCCAAAGATTTTGATGGGATTGCACTATAATTCGTTTTGCCGACATTAAAAGTTACTCAAACATACAGTGATGAGTGGAAACCGTAGAAGCCTCTGTCTAGGGGAAGAAATGTTGCTTTTGTTTGAATTAATGAATTTCTTCCAATTATTTTTTATCAAGCAGGTTTCATATGGGTAGGCAGCCGTTGATAATAATAGCAGACCCTGAGCTTTGTAAAGAAGTCGGTATCAAGAAATTCAAGGACATCCCAAATAGAAGTGTCCCTTCTCCAATATCAGCTTCCCCGCTTCATCAGAAAGGTCTTTTCTTCACAAGGTACTTTCTATCTTTTTCTTAAGAGGAGAAAAAACGCTCTATCAACTGCTTATTAACGCCTCTATTCAATTTTACAGGGATGCAAGATGGTCGACAATGCGAAACACGATACTATCGGTCTATCAGCCCTCTCATCTAACGAGCCTAATACCTACTATGCAATCAATCATTGAAACTGCAACTCAAAATCTCCATTCCTCTGTCGAGGAAGATATCCCTTTCTCCAATCTCTCCCTCAAATTGACCACTGATGTGATTGGAACGGCAGCCTTCGGTGTTAACTTTGGGCTCTCTAATCCACAAGCAACCAAAACTTGTGGTACCAATGGCCATCATGACAGCAAAACTGATGAAGTTTCGGACTTCATCAATCAACATATCTACTCCACAACGCAGCTCAAGATGGATTTATCAGGTTCCTTCTCAATCATTTTGGGACTACTTGTCCCTATACTCCAAGAACCATTTAGACAAATCCTGAAGAGAATACCATTCACTATGGACTGGAAAGTTGATCGGACAAATCAGAAGTTGAGTGGTCGGCTTAATGAGATTGTGGACAAGAGAATGAAGTGTAACGATCGAGGTTCAAAAGACTTCTTATCACTCATTTTGAGAGCAAGAGAGTCAGAGACGGTGTCAAGGAATGTCTTCACTCCAGACTACATCAGTGCAGTCACGTACGAACACCTACTTGCTGGGTCAGCTACCACGGCATTCACATTGTCTTCTATTGTATATTTAATTGCTGGACATCCAGAAGTCGAGAAGAAGTTGCTTGAAGAGATTGACAACTTTGGTCCATCAGATCAGATACCAACAGCTAATGATCTTCAGCAGAAGTTTCCATATCTTGATCAGGTTAAGTAACTTAGGCTATTGTTCTCGTTTCTATGGTTTTAGACCTCTCACATGAAGGAGTTTCATTGTTTTCATAATCAAATGAGTGCAGGTGATTAAAGAGGCTATGAGGTTCTACACTGTTTCCCCTCTAGTAGCTAGGGAGACGTCTAAAGATGTGGAGATTGGTGGATATCTTCTTCCAAAGGTAATTCTATTAAAAAAAAAAAACCCATCTGATGCATCTTTGTGTTCAAGCAACGTAGTCGATCAGTCTAACTAATGGAAAGACACTACTTTGTGATGAGATTAGGGGACATGGGTTTGGTTAGCACTTGGAGTTCTTGCCAAGGATCCAAAGAACTTTCCAGAACCAGATCAATTTAAGCCAGAGAGGTTTGATCCGAATGAGGAAGAGGAGAAACAAAGGCACCCCTATGCTTTAATCCCATTTGGAATTGGTCCTCGAGCATGCATTGGTCAAAAATTCGCCCTTCAGGAATTGAAGCTCTCTCTGATTCATTTGTATAGGAAGTTTGTATTTCGGCATTCCCTCTCCATGGAACAACCTCTTGAACTTGAGTATGGTGTCATTCTCAACTTCAAGAATGGTGTCAAGCTCAGAGTGATAAATCGTAAATAAAGGGCATCACAATGACTATGGTTTTGCTATCAGTCAGTTTGTACGCTAAGTATCAATCATCAAAACTATGTCAATAGAAGTTTATGTATGTTTCTAAAATATAAATTATCATTTTAGGATTCCAATATCATATTCAATGGTTTTAGTTACTTTTTGACATTACATAAACAGCAAATCTTCTTTAATCGAAAAGGACTCCCATATTTCATCCATCCATTGAAAATATAAGCATCCTTGATAGTTGCCATCAAAGAAATCAGGAGCATATCAGATCATTTTGACATTATTGCACTACACGTCTATTCCATCCAGATAAGATAAGCTTTGGCCAAGAGTTCTAGACAGAATTTGCAGAAGTGGGAAAAAAACAAGATGAAAACCAATGATCACCAAGAGCTATAATACAAAGAACGAGCCATTCTCCATTGGAAGTGAAACAATACCAACTCTGTCGAATGATACACAATTTTGATGGCAACAATTAAGAAACAAGGCAGTGCAGTAACTCTTCTAGAATTACCAGAAACAGGTTTCATCTCAAAGCTGAATAAGTGAGCCTCTGTCTAATTGCTCATGACCTTAGCTTTGTTCCATTCTTGTCCAATCGTGTTGGCAGCATCAATGGCTTAAAGGGATTGACTGATGAATCAGATCCGGCACTGCTCTTAGAATCCCCATTTGAG

mRNA sequence

AGATGGGATTGAGTTTTACTCTTCCACTAGAGGCTTCTTTTGCTGCTGAGTTTGTTTAATGTAATGGTCGAATTGAATTTTTGTGTTAAAAACATTATGAAAAAAGCGAAGTAGGCTGCAGAATATGATGAATTCTTCACCATCCAAACATTTCAACGATGCAGAATAACGAAAGAATAAAAGGACTTCAATGGTCGTTTCATTATTTCCACTCCAATAATCATTCGTGTACCGACAAAAGTACCTACCTTAGTGAAACTCCCACTGCCACCCCCTCCTTTCCATTTTCATTTCCTCACCATTTGCCTTATTTAAACACAAATGAAACACCACAACCAAATAAACTCTCAACAGTCAACGCAGTCCCTCCCTTTCCCTTTCTCCTTCTTCTCTCTCCCTTTTCCTCGGTTTGGGTATCGAGAAAAATGGATGATTTTCTTCAATCAGTGTTGGCGAGTGTGTCTATGGGAGTGGGTGCAATATTGTTCACGACGTTGTGCGTCGTGGGAGGTGGGTTGGTTTATTTCTATGGACCTTACTGGGGAGTGAGAAGGGTGCCTGGGCCACCAGCTATTCCGCTGGTCGGACACCTTCCCTTACTGGCCAAGTACGGCCCAGACGTTTTCTCTGTCCTCGCCTCCCAATATGGTCCTATCTTCAGGTTTCATATGGGTAGGCAGCCGTTGATAATAATAGCAGACCCTGAGCTTTGTAAAGAAGTCGGTATCAAGAAATTCAAGGACATCCCAAATAGAAGTGTCCCTTCTCCAATATCAGCTTCCCCGCTTCATCAGAAAGGTCTTTTCTTCACAAGAGGAGAAAAAACGCTCTATCAACTGCTTATTAACGCCTCTATTCAATTTTACAGGGATGCAAGATGGTCGACAATGCGAAACACGATACTATCGGTCTATCAGCCCTCTCATCTAACGAGCCTAATACCTACTATGCAATCAATCATTGAAACTGCAACTCAAAATCTCCATTCCTCTGTCGAGGAAGATATCCCTTTCTCCAATCTCTCCCTCAAATTGACCACTGATGTGATTGGAACGGCAGCCTTCGGTGTTAACTTTGGGCTCTCTAATCCACAAGCAACCAAAACTTGTGGTACCAATGGCCATCATGACAGCAAAACTGATGAAGTTTCGGACTTCATCAATCAACATATCTACTCCACAACGCAGCTCAAGATGGATTTATCAGGTTCCTTCTCAATCATTTTGGGACTACTTGTCCCTATACTCCAAGAACCATTTAGACAAATCCTGAAGAGAATACCATTCACTATGGACTGGAAAGTTGATCGGACAAATCAGAAGTTGAGTGGTCGGCTTAATGAGATTGTGGACAAGAGAATGAAGTGTAACGATCGAGGTTCAAAAGACTTCTTATCACTCATTTTGAGAGCAAGAGAGTCAGAGACGGTGTCAAGGAATGTCTTCACTCCAGACTACATCAGTGCAGTCACGTACGAACACCTACTTGCTGGGTCAGCTACCACGGCATTCACATTGTCTTCTATTGTATATTTAATTGCTGGACATCCAGAAGTCGAGAAGAAGTTGCTTGAAGAGATTGACAACTTTGGTCCATCAGATCAGATACCAACAGCTAATGATCTTCAGCAGAAGTTTCCATATCTTGATCAGGTGATTAAAGAGGCTATGAGGTTCTACACTGTTTCCCCTCTAGTAGCTAGGGAGACGTCTAAAGATGTGGAGATTGGTGGATATCTTCTTCCAAAGGGGACATGGGTTTGGTTAGCACTTGGAGTTCTTGCCAAGGATCCAAAGAACTTTCCAGAACCAGATCAATTTAAGCCAGAGAGGTTTGATCCGAATGAGGAAGAGGAGAAACAAAGGCACCCCTATGCTTTAATCCCATTTGGAATTGGTCCTCGAGCATGCATTGGTCAAAAATTCGCCCTTCAGGAATTGAAGCTCTCTCTGATTCATTTGTATAGGAAGTTTGTATTTCGGCATTCCCTCTCCATGGAACAACCTCTTGAACTTGAGTATGGTGTCATTCTCAACTTCAAGAATGGTGTCAAGCTCAGAGTGATAAATCGTAAATAAAGGGCATCACAATGACTATGGTTTTGCTATCAGTCAGTTTGTACGCTAAGTATCAATCATCAAAACTATGTCAATAGAAGTTTATGTATGTTTCTAAAATATAAATTATCATTTTAGGATTCCAATATCATATTCAATGGTTTTAGTTACTTTTTGACATTACATAAACAGCAAATCTTCTTTAATCGAAAAGGACTCCCATATTTCATCCATCCATTGAAAATATAAGCATCCTTGATAGTTGCCATCAAAGAAATCAGGAGCATATCAGATCATTTTGACATTATTGCACTACACGTCTATTCCATCCAGATAAGATAAGCTTTGGCCAAGAGTTCTAGACAGAATTTGCAGAAGTGGGAAAAAAACAAGATGAAAACCAATGATCACCAAGAGCTATAATACAAAGAACGAGCCATTCTCCATTGGAAGTGAAACAATACCAACTCTGTCGAATGATACACAATTTTGATGGCAACAATTAAGAAACAAGGCAGTGCAGTAACTCTTCTAGAATTACCAGAAACAGGTTTCATCTCAAAGCTGAATAAGTGAGCCTCTGTCTAATTGCTCATGACCTTAGCTTTGTTCCATTCTTGTCCAATCGTGTTGGCAGCATCAATGGCTTAAAGGGATTGACTGATGAATCAGATCCGGCACTGCTCTTAGAATCCCCATTTGAG

Coding sequence (CDS)

ATGCAGAATAACGAAAGAATAAAAGGACTTCAATGGTCGTTTCATTATTTCCACTCCAATAATCATTCGTGTACCGACAAAAGTACCTACCTTAGTGAAACTCCCACTGCCACCCCCTCCTTTCCATTTTCATTTCCTCACCATTTGCCTTATTTAAACACAAATGAAACACCACAACCAAATAAACTCTCAACAGTCAACGCAGTCCCTCCCTTTCCCTTTCTCCTTCTTCTCTCTCCCTTTTCCTCGGTTTGGGTATCGAGAAAAATGGATGATTTTCTTCAATCAGTGTTGGCGAGTGTGTCTATGGGAGTGGGTGCAATATTGTTCACGACGTTGTGCGTCGTGGGAGGTGGGTTGGTTTATTTCTATGGACCTTACTGGGGAGTGAGAAGGGTGCCTGGGCCACCAGCTATTCCGCTGGTCGGACACCTTCCCTTACTGGCCAAGTACGGCCCAGACGTTTTCTCTGTCCTCGCCTCCCAATATGGTCCTATCTTCAGGTTTCATATGGGTAGGCAGCCGTTGATAATAATAGCAGACCCTGAGCTTTGTAAAGAAGTCGGTATCAAGAAATTCAAGGACATCCCAAATAGAAGTGTCCCTTCTCCAATATCAGCTTCCCCGCTTCATCAGAAAGGTCTTTTCTTCACAAGAGGAGAAAAAACGCTCTATCAACTGCTTATTAACGCCTCTATTCAATTTTACAGGGATGCAAGATGGTCGACAATGCGAAACACGATACTATCGGTCTATCAGCCCTCTCATCTAACGAGCCTAATACCTACTATGCAATCAATCATTGAAACTGCAACTCAAAATCTCCATTCCTCTGTCGAGGAAGATATCCCTTTCTCCAATCTCTCCCTCAAATTGACCACTGATGTGATTGGAACGGCAGCCTTCGGTGTTAACTTTGGGCTCTCTAATCCACAAGCAACCAAAACTTGTGGTACCAATGGCCATCATGACAGCAAAACTGATGAAGTTTCGGACTTCATCAATCAACATATCTACTCCACAACGCAGCTCAAGATGGATTTATCAGGTTCCTTCTCAATCATTTTGGGACTACTTGTCCCTATACTCCAAGAACCATTTAGACAAATCCTGAAGAGAATACCATTCACTATGGACTGGAAAGTTGATCGGACAAATCAGAAGTTGAGTGGTCGGCTTAATGAGATTGTGGACAAGAGAATGAAGTGTAACGATCGAGGTTCAAAAGACTTCTTATCACTCATTTTGAGAGCAAGAGAGTCAGAGACGGTGTCAAGGAATGTCTTCACTCCAGACTACATCAGTGCAGTCACGTACGAACACCTACTTGCTGGGTCAGCTACCACGGCATTCACATTGTCTTCTATTGTATATTTAATTGCTGGACATCCAGAAGTCGAGAAGAAGTTGCTTGAAGAGATTGACAACTTTGGTCCATCAGATCAGATACCAACAGCTAATGATCTTCAGCAGAAGTTTCCATATCTTGATCAGGTGATTAAAGAGGCTATGAGGTTCTACACTGTTTCCCCTCTAGTAGCTAGGGAGACGTCTAAAGATGTGGAGATTGGTGGATATCTTCTTCCAAAGGGGACATGGGTTTGGTTAGCACTTGGAGTTCTTGCCAAGGATCCAAAGAACTTTCCAGAACCAGATCAATTTAAGCCAGAGAGGTTTGATCCGAATGAGGAAGAGGAGAAACAAAGGCACCCCTATGCTTTAATCCCATTTGGAATTGGTCCTCGAGCATGCATTGGTCAAAAATTCGCCCTTCAGGAATTGAAGCTCTCTCTGATTCATTTGTATAGGAAGTTTGTATTTCGGCATTCCCTCTCCATGGAACAACCTCTTGAACTTGAGTATGGTGTCATTCTCAACTTCAAGAATGGTGTCAAGCTCAGAGTGATAAATCGTAAATAA

Protein sequence

MQNNERIKGLQWSFHYFHSNNHSCTDKSTYLSETPTATPSFPFSFPHHLPYLNTNETPQPNKLSTVNAVPPFPFLLLLSPFSSVWVSRKMDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKLRVINRK
Homology
BLAST of Lsi04G014190 vs. ExPASy Swiss-Prot
Match: B9DFU2 (Cytochrome P450 711A1 OS=Arabidopsis thaliana OX=3702 GN=CYP711A1 PE=2 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 5.7e-219
Identity = 382/519 (73.60%), Postives = 433/519 (83.43%), Query Frame = 0

Query: 120 LVYFYGPYWGVRRVPGPPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIII 179
           ++Y Y P W V  VPGP A+PLVGHLPL+AKYGPDVFSVLA QYGPIFRF MGRQPLIII
Sbjct: 33  VIYLYRPSWSVCNVPGPTAMPLVGHLPLMAKYGPDVFSVLAKQYGPIFRFQMGRQPLIII 92

Query: 180 ADPELCKEVGIKKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDA 239
           A+ ELC+EVGIKKFKD+PNRS+PSPISASPLH+KGLFFT                  RD 
Sbjct: 93  AEAELCREVGIKKFKDLPNRSIPSPISASPLHKKGLFFT------------------RDK 152

Query: 240 RWSTMRNTILSVYQPSHLTSLIPTMQSIIETATQNLHSSVEEDIPFSNLSLKLTTDVIGT 299
           RWS MRNTILS+YQPSHLTSLIPTM S I +AT NL S    DI FSNL LKLTTD+IG 
Sbjct: 153 RWSKMRNTILSLYQPSHLTSLIPTMHSFITSATHNLDSK-PRDIVFSNLFLKLTTDIIGQ 212

Query: 300 AAFGVNFGLSNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSIILGLL 359
           AAFGV+FGLS           G    K  EV+DFINQH+YSTTQLKMDLSGS SIILGLL
Sbjct: 213 AAFGVDFGLS-----------GKKPIKDVEVTDFINQHVYSTTQLKMDLSGSLSIILGLL 272

Query: 360 VPILQEPFRQILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGSKDFLSLILRAR 419
           +PILQEPFRQ+LKRIP TMDW+V++TN +LSG+LNEIV KR K  +  SKDFLSLIL+AR
Sbjct: 273 IPILQEPFRQVLKRIPGTMDWRVEKTNARLSGQLNEIVSKRAKEAETDSKDFLSLILKAR 332

Query: 420 ESETVSRNVFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEIDNFGP 479
           ES+  ++N+FT DYISAVTYEHLLAGSATTAFTLSS++YL++GH +VEK+LL+EID FG 
Sbjct: 333 ESDPFAKNIFTSDYISAVTYEHLLAGSATTAFTLSSVLYLVSGHLDVEKRLLQEIDGFGN 392

Query: 480 SDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLVARETSKDVEIGGYLLPKGTWVWLALG 539
            D IPTA+DLQ KFPYLDQVIKEAMRFY VSPLVARET+K+VEIGGYLLPKGTWVWLALG
Sbjct: 393 RDLIPTAHDLQHKFPYLDQVIKEAMRFYMVSPLVARETAKEVEIGGYLLPKGTWVWLALG 452

Query: 540 VLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFALQELKLSLI 599
           VLAKDPKNFPEP++FKPERFDPN EEEK RHPYA IPFGIGPRAC+GQ+FALQE+KL+L+
Sbjct: 453 VLAKDPKNFPEPEKFKPERFDPNGEEEKHRHPYAFIPFGIGPRACVGQRFALQEIKLTLL 512

Query: 600 HLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKLRVINR 639
           HLYR ++FRHSL ME PL+L+YG+IL+FKNGVKLR I R
Sbjct: 513 HLYRNYIFRHSLEMEIPLQLDYGIILSFKNGVKLRTIKR 521

BLAST of Lsi04G014190 vs. ExPASy Swiss-Prot
Match: P47787 (Thromboxane-A synthase OS=Sus scrofa OX=9823 GN=TBXAS1 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 9.3e-52
Identity = 149/541 (27.54%), Postives = 244/541 (45.10%), Query Frame = 0

Query: 136 PPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKD 195
           P   P +G+L    +   +    L  QYGP+  +++GR+ +++I+DP++ K+V  +KF +
Sbjct: 47  PKPSPFIGNLTFFRQGFWESHMELRKQYGPLSGYYLGRRMIVVISDPDMIKQVLAEKFSN 106

Query: 196 IPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPS 255
             NR       A+ L  K               +  SI F RD RW  +R+ + S + P 
Sbjct: 107 FTNR------MATGLESKP--------------VADSILFLRDKRWEEVRSVLTSAFSPK 166

Query: 256 HLTSLIPTMQSIIETATQNLHSSVEEDIPF--SNLSLKLTTDVIGTAAFGVNFGLSNPQA 315
            L  L P +    +    +L    E    F         TTDV+ + AFG          
Sbjct: 167 KLNKLTPLISQACDLLLAHLERYAESGDAFDIQRCYCCYTTDVVASVAFGTQ-------- 226

Query: 316 TKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKR 375
                 N   + +   V        +S  +L         ++L L  P +  P  +IL  
Sbjct: 227 -----VNSSEEPEHPFVKHCRRFFAFSVPRL--------ILVLILSFPSIMVPLARILP- 286

Query: 376 IPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGS-----KDFLSLILRAR--------E 435
                    ++   +++G  N+++   +   D+ +     +DFL ++L  R        E
Sbjct: 287 ---------NKKRDEVNGFFNKLIRNVIALRDQQAAEERRQDFLQMVLDLRHSAPSVGVE 346

Query: 436 SETVSRNVF-----------------------TPDYISAVTYEHLLAGSATTAFTLSSIV 495
           +  + R  F                       T D +    +  L+AG      TLS + 
Sbjct: 347 NFDIVRQAFSSAKGCPADPSQPHLPRPLSKPLTVDEVVGQAFLFLIAGYEIITNTLSFVT 406

Query: 496 YLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLVARET 555
           YL+A +P+ ++KLL E+D+F      P    LQQ  PYLD V+ E +R Y  +    RE 
Sbjct: 407 YLLATNPDCQEKLLREVDDFSKKHPSPEHCSLQQGLPYLDMVLSETLRMYPPAFRFTREA 466

Query: 556 SKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPF 615
           ++D E+ G  +P GT + +A+G L  DPK++P P+ F PERF    E ++ + P+  +PF
Sbjct: 467 ARDCEVLGQRIPAGTVLEVAVGALHHDPKHWPHPETFDPERF--TAEAQRLQQPFTYLPF 526

Query: 616 GIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKLRVIN 639
           G GPR+C+G +  L E+KL+L+H+ RKF F      + PL+LE    L+ KNGV +R++ 
Sbjct: 527 GAGPRSCLGVQLGLLEIKLTLLHILRKFRFEACPETQVPLQLESKSALSPKNGVYIRIVP 534

BLAST of Lsi04G014190 vs. ExPASy Swiss-Prot
Match: Q2KIG5 (Thromboxane-A synthase OS=Bos taurus OX=9913 GN=TBXAS1 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 5.1e-50
Identity = 147/545 (26.97%), Postives = 241/545 (44.22%), Query Frame = 0

Query: 136 PPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKD 195
           P   P +G+L    +   +    L  QYGP+  +++GR   I+I++P++ ++V ++KF +
Sbjct: 47  PKPSPFIGNLAFFRQGFWESHMELRKQYGPLSGYYLGRLMFIVISEPDMIEQVLVEKFSN 106

Query: 196 IPNRSV----PSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSV 255
             NR      P P++ S L                        F RD RW  +R+ +   
Sbjct: 107 FTNRMATGLEPKPVADSVL------------------------FLRDKRWEEVRSVLTVA 166

Query: 256 YQPSHLTSLIPTMQSIIETATQNL--HSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLS 315
           + P  L+ + P +    +    +L  H+   E           TTDV+ + AFG      
Sbjct: 167 FSPEKLSEMTPLISRACDVLLAHLERHAQSGEAFDIQRTYCCYTTDVVASVAFGTEV--- 226

Query: 316 NPQATKTCGTNGHHDSKTDEVSDFINQ-HIYSTTQLKMDLSGSFSIILGLLVPILQEPFR 375
                         +S+      F+     +  + +   L     ++L L  P +  P  
Sbjct: 227 --------------NSQEAPEHPFVEHCRRFFASSIPKPL-----LVLLLSFPSIMVPLA 286

Query: 376 QILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGS-----KDFLSLILRARESET 435
           +IL           ++   +L+G  N+++   +   D+ +     +DFL ++   R S  
Sbjct: 287 RILP----------NKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVQDVRHSAA 346

Query: 436 --------VSRNVFTP----------------------DYISAVTYEHLLAGSATTAFTL 495
                   + R VF+                       D +    +  L+AG      TL
Sbjct: 347 TVGVEKFDIVRQVFSATKCPANPPRRHSPRPLSKPLSVDEVVGQAFIFLIAGYEIVTNTL 406

Query: 496 SSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLV 555
           S   YL+A +PE ++KLLEE+D F      P    LQ+  PYLD VIKE +R Y  +   
Sbjct: 407 SFATYLLATNPECQEKLLEEVDCFSKEHLAPEYCSLQEGLPYLDMVIKETLRMYPPAFRF 466

Query: 556 ARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYA 615
            R  ++D E+ G  +P G  +  A+G L  DP+++P P+ F PERF    E +++R PY 
Sbjct: 467 TRVAAQDCEVLGQRIPAGAVLETAVGALHYDPEHWPNPENFNPERF--TAEAQQRRRPYT 526

Query: 616 LIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKL 639
            +PFG GPR+C+G +  L ELKL+L+H+ RKF F      + PL+LE    L  KNGV +
Sbjct: 527 YLPFGAGPRSCLGVRLGLLELKLTLLHILRKFRFEACPETQVPLQLESKSALGPKNGVYI 533

BLAST of Lsi04G014190 vs. ExPASy Swiss-Prot
Match: P49430 (Thromboxane-A synthase OS=Rattus norvegicus OX=10116 GN=Tbxas1 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 8.7e-50
Identity = 159/557 (28.55%), Postives = 254/557 (45.60%), Query Frame = 0

Query: 106 GAILFTTLCVVGGGLVYFY--GPYWGVRR--VPGPPAIPLVGHLPLLAKYGPDVFSVLAS 165
           G ++  TL VV   L+ +Y    +  +R+  +  P   P VG+L    +   +    L  
Sbjct: 13  GTVVTVTLSVVLLALLKWYSTSAFSRLRKLGIRHPEPSPFVGNLMFFRQGFWESHLELRE 72

Query: 166 QYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASPLHQKGLFFTRGE 225
           +YGP+  +++GR+  I+I+DP++ KEV ++ F +  NR       AS L  K        
Sbjct: 73  RYGPLCGYYLGRRMYIVISDPDMIKEVLVENFSNFSNR------MASGLEPK-------- 132

Query: 226 KTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIETATQNL-HSSVE 285
                 LI  S+   RD RW  +R  ++S + P  L  + P +    E    +L HS+  
Sbjct: 133 ------LIADSVLMLRDRRWEEVRGALMSAFSPEKLNEMTPLISQACELLLSHLKHSAAS 192

Query: 286 ED-IPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDEVSDFINQHIY 345
            D           TT+V+ + AFG+               N     +   V        +
Sbjct: 193 GDAFDIQRCYCCFTTNVVASVAFGIE-------------VNSQDAPEDPFVQHCQRVFAF 252

Query: 346 STTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKLSGR------L 405
           ST +  + L  SF  I+  L  IL    R  L     T+   V     K +        L
Sbjct: 253 STPRPLLALILSFPSIMVPLARILPNKNRDELNGFFNTLIRNVIALRDKQTAEERRGDFL 312

Query: 406 NEIVDKRMKCNDRGSKDFLSLILRARES------------ETVSRNVFTPDYISAVTYEH 465
             ++D +   +  G + F  ++  A  S             T +    T D I+   +  
Sbjct: 313 QMVLDAQRSMSSVGVEAF-DMVTEALSSAECMGDPPQRCHPTSTAKPLTVDEIAGQAFLF 372

Query: 466 LLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIK 525
           L+AG   T  TLS I YL+A HPE +++LL+E+D F      P   +LQ+  PYLD V+ 
Sbjct: 373 LIAGHEITTNTLSFITYLLATHPECQERLLKEVDLFMEKHPAPEYCNLQEGLPYLDMVVA 432

Query: 526 EAMRFYTVSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDP 585
           E +R Y  +    RE ++D E+ G  +P G+ + +A+G L  DP+++P P+ F PERF  
Sbjct: 433 ETLRMYPPAFRFTREAAQDCEVLGQHIPAGSVLEIAVGALHHDPEHWPNPETFDPERF-- 492

Query: 586 NEEEEKQRHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEY 639
             E   Q+ P+  +PFG GPR+C+G +  L  +KL+L+ +  KF F      + PL+LE 
Sbjct: 493 TAEARLQQKPFTYLPFGAGPRSCLGVRLGLLVVKLTLLQVLHKFRFEACPETQVPLQLES 533

BLAST of Lsi04G014190 vs. ExPASy Swiss-Prot
Match: P24557 (Thromboxane-A synthase OS=Homo sapiens OX=9606 GN=TBXAS1 PE=1 SV=3)

HSP 1 Score: 197.2 bits (500), Expect = 5.6e-49
Identity = 146/540 (27.04%), Postives = 238/540 (44.07%), Query Frame = 0

Query: 136 PPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKD 195
           P   P +G+L    +   +    L   YGP+  +++GR+  I+I++P++ K+V ++ F +
Sbjct: 47  PKPSPFIGNLTFFRQGFWESQMELRKLYGPLCGYYLGRRMFIVISEPDMIKQVLVENFSN 106

Query: 196 IPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPS 255
             NR       AS L  K               +  S+ F RD RW  +R  ++S + P 
Sbjct: 107 FTNR------MASGLEFKS--------------VADSVLFLRDKRWEEVRGALMSAFSPE 166

Query: 256 HLTSLIPTMQSIIETATQNLHSSVEEDIPF--SNLSLKLTTDVIGTAAFGVNFGLSNPQA 315
            L  ++P +    +    +L    E    F         TTDV+ + AFG          
Sbjct: 167 KLNEMVPLISQACDLLLAHLKRYAESGDAFDIQRCYCNYTTDVVASVAFG---------- 226

Query: 316 TKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKR 375
           T         D        F    I               ++L L  P +  P  +IL  
Sbjct: 227 TPVDSWQAPEDPFVKHCKRFFEFCIPRPI-----------LVLLLSFPSIMVPLARILP- 286

Query: 376 IPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGS-----KDFLSLILRARESET----- 435
                    ++   +L+G  N+++   +   D+ +     +DFL ++L AR S +     
Sbjct: 287 ---------NKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVLDARHSASPMGVQ 346

Query: 436 ---VSRNVF----------------------TPDYISAVTYEHLLAGSATTAFTLSSIVY 495
              + R+VF                      T D I    +  L+AG      TLS   Y
Sbjct: 347 DFDIVRDVFSSTGCKPNPSRQHQPSPMARPLTVDEIVGQAFIFLIAGYEIITNTLSFATY 406

Query: 496 LIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLVARETS 555
           L+A +P+ ++KLL E+D F      P    L++  PYLD VI E +R Y  +    RE +
Sbjct: 407 LLATNPDCQEKLLREVDVFKEKHMAPEFCSLEEGLPYLDMVIAETLRMYPPAFRFTREAA 466

Query: 556 KDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFG 615
           +D E+ G  +P G  + +A+G L  DP+++P P+ F PERF    E  +Q  P+  +PFG
Sbjct: 467 QDCEVLGQRIPAGAVLEMAVGALHHDPEHWPSPETFNPERF--TAEARQQHRPFTYLPFG 526

Query: 616 IGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKLRVINR 639
            GPR+C+G +  L E+KL+L+H+  KF F+     + PL+LE    L  KNGV +++++R
Sbjct: 527 AGPRSCLGVRLGLLEVKLTLLHVLHKFRFQACPETQVPLQLESKSALGPKNGVYIKIVSR 533

BLAST of Lsi04G014190 vs. ExPASy TrEMBL
Match: A0A6J1F8E6 (cytochrome P450 711A1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443047 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 5.8e-291
Identity = 510/550 (92.73%), Postives = 521/550 (94.73%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD F ++V+ASV +GVGA+LFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDGFFRTVVASVPVGVGALLFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG  DSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG-RDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIP TMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPSTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDR SKDFLSLILRARESETVSRNVF+PDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRSSKDFLSLILRARESETVSRNVFSPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSSIVYL+AGHPEVEKKLLEEIDN+GPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV
Sbjct: 361 AFTLSSIVYLVAGHPEVEKKLLEEIDNYGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDP+ EEEKQR
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPDAEEEKQR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKL+LIHLYRKF FRHSLSMEQPLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLTLIHLYRKFAFRHSLSMEQPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. ExPASy TrEMBL
Match: A0A1S3BUH6 (cytochrome P450 711A1 OS=Cucumis melo OX=3656 GN=LOC103493668 PE=3 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.3e-290
Identity = 508/550 (92.36%), Postives = 519/550 (94.36%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD+  QS++ASV MGVG ILFTT C+VGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDNLFQSMVASVPMGVGTILFTTFCLVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDI NRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDISNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNP ATKTCGTN   DSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPHATKTCGTN-DQDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVP+LQEPFRQ+LKRIPFTMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPVLQEPFRQVLKRIPFTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSS+VYL+AGHPEVEKKLLEEIDNFGPSDQIPTANDLQQ FPYLDQVIKE+MRFYTV
Sbjct: 361 AFTLSSVVYLVAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQMFPYLDQVIKESMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDPNEEEEK+R
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPNEEEEKRR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. ExPASy TrEMBL
Match: A0A6J1IH85 (cytochrome P450 711A1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477201 PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.2e-290
Identity = 508/550 (92.36%), Postives = 519/550 (94.36%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD F ++V+ASV +GVGA+LFTTLCVVGGG VYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDGFFRTVVASVPVGVGALLFTTLCVVGGGFVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG HDSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG-HDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIP TMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPSTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDR SKDFLSLILRARESETVS+NVF+PDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRSSKDFLSLILRARESETVSKNVFSPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSSIVYL+AGHPEVEKKLLEEIDN+GPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV
Sbjct: 361 AFTLSSIVYLVAGHPEVEKKLLEEIDNYGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD FKPERFDP+ EEEKQR
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDNFKPERFDPDAEEEKQR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKL+LIHLYRKF FRHSLSME PLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLTLIHLYRKFAFRHSLSMEHPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. ExPASy TrEMBL
Match: A0A5D3D954 (Cytochrome P450 711A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002090 PE=3 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.2e-289
Identity = 508/551 (92.20%), Postives = 519/551 (94.19%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD+  QS++ASV MGVG ILFTT C+VGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDNLFQSMVASVPMGVGTILFTTFCLVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIF-RFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISAS 209
           KYGPDVFSVLASQYGPIF RFHMGRQPLIIIADPELCKEVGIKKFKDI NRSVPSPISAS
Sbjct: 61  KYGPDVFSVLASQYGPIFSRFHMGRQPLIIIADPELCKEVGIKKFKDISNRSVPSPISAS 120

Query: 210 PLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSII 269
           PLHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSII
Sbjct: 121 PLHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSII 180

Query: 270 ETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTD 329
           ETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNP ATKTCGTN   DSK D
Sbjct: 181 ETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPHATKTCGTN-DQDSKND 240

Query: 330 EVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQK 389
           EVSDFINQHIYSTTQLKMDLSGSFSIILGLLVP+LQEPFRQ+LKRIPFTMDWKVDRTNQK
Sbjct: 241 EVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPVLQEPFRQVLKRIPFTMDWKVDRTNQK 300

Query: 390 LSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSAT 449
           LSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSAT
Sbjct: 301 LSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSAT 360

Query: 450 TAFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYT 509
           TAFTLSS+VYL+AGHPEVEKKLLEEIDNFGPSDQIPTANDLQQ FPYLDQVIKE+MRFYT
Sbjct: 361 TAFTLSSVVYLVAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQMFPYLDQVIKESMRFYT 420

Query: 510 VSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQ 569
           VSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDPNEEEEK+
Sbjct: 421 VSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPNEEEEKR 480

Query: 570 RHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK 629
           RHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK
Sbjct: 481 RHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK 532

Query: 630 NGVKLRVINRK 640
           NGVKLRVINRK
Sbjct: 541 NGVKLRVINRK 532

BLAST of Lsi04G014190 vs. ExPASy TrEMBL
Match: A0A0A0L0Q6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646170 PE=3 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 3.5e-288
Identity = 505/550 (91.82%), Postives = 516/550 (93.82%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD+F QS++ASV MGVG ILFTT C+VGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDNFFQSMVASVPMGVGTILFTTFCLVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSV ASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP
Sbjct: 61  KYGPDVFSVFASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHL  LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLMRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNP ATK   T    DSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPHATK---TTNDQDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQ+LKRIPFTMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQVLKRIPFTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSS+VYL+AGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKE+MRFYTV
Sbjct: 361 AFTLSSVVYLVAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKESMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDPNEEEEK+R
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPNEEEEKRR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK+
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKS 529

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 529

BLAST of Lsi04G014190 vs. NCBI nr
Match: XP_023536371.1 (cytochrome P450 711A1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1010.7 bits (2612), Expect = 5.4e-291
Identity = 510/550 (92.73%), Postives = 521/550 (94.73%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD F ++V+ASV +GVGA+LFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDGFFRTVVASVPVGVGALLFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG HDSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG-HDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIP TMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPSTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDR SKDFLSLILRARESETVSRNVF+PDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRSSKDFLSLILRARESETVSRNVFSPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSSIVYL+AGHPEVEKKLLEEIDN+GPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV
Sbjct: 361 AFTLSSIVYLVAGHPEVEKKLLEEIDNYGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDP+ EEEKQR
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPDAEEEKQR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKL+LIHLYRKF FRHSLSME PLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLTLIHLYRKFAFRHSLSMEHPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. NCBI nr
Match: XP_022936422.1 (cytochrome P450 711A1 isoform X1 [Cucurbita moschata] >KAG7024349.1 Cytochrome P450 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1009.6 bits (2609), Expect = 1.2e-290
Identity = 510/550 (92.73%), Postives = 521/550 (94.73%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD F ++V+ASV +GVGA+LFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDGFFRTVVASVPVGVGALLFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG  DSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG-RDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIP TMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPSTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDR SKDFLSLILRARESETVSRNVF+PDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRSSKDFLSLILRARESETVSRNVFSPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSSIVYL+AGHPEVEKKLLEEIDN+GPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV
Sbjct: 361 AFTLSSIVYLVAGHPEVEKKLLEEIDNYGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDP+ EEEKQR
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPDAEEEKQR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKL+LIHLYRKF FRHSLSMEQPLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLTLIHLYRKFAFRHSLSMEQPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. NCBI nr
Match: XP_008452735.1 (PREDICTED: cytochrome P450 711A1 [Cucumis melo])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.7e-290
Identity = 508/550 (92.36%), Postives = 519/550 (94.36%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD+  QS++ASV MGVG ILFTT C+VGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDNLFQSMVASVPMGVGTILFTTFCLVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDI NRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDISNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNP ATKTCGTN   DSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPHATKTCGTN-DQDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVP+LQEPFRQ+LKRIPFTMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPVLQEPFRQVLKRIPFTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSS+VYL+AGHPEVEKKLLEEIDNFGPSDQIPTANDLQQ FPYLDQVIKE+MRFYTV
Sbjct: 361 AFTLSSVVYLVAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQMFPYLDQVIKESMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDPNEEEEK+R
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPNEEEEKRR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. NCBI nr
Match: XP_022977002.1 (cytochrome P450 711A1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1007.7 bits (2604), Expect = 4.6e-290
Identity = 508/550 (92.36%), Postives = 519/550 (94.36%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD F ++V+ASV +GVGA+LFTTLCVVGGG VYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDGFFRTVVASVPVGVGALLFTTLCVVGGGFVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 209
           KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP
Sbjct: 61  KYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASP 120

Query: 210 LHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIE 269
           LHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSIIE
Sbjct: 121 LHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSIIE 180

Query: 270 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDE 329
           TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG HDSK DE
Sbjct: 181 TATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNG-HDSKNDE 240

Query: 330 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKL 389
           VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIP TMDWKVDRTNQKL
Sbjct: 241 VSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPSTMDWKVDRTNQKL 300

Query: 390 SGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATT 449
           SGRLNEIVDKRMKCNDR SKDFLSLILRARESETVS+NVF+PDYISAVTYEHLLAGSATT
Sbjct: 301 SGRLNEIVDKRMKCNDRSSKDFLSLILRARESETVSKNVFSPDYISAVTYEHLLAGSATT 360

Query: 450 AFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 509
           AFTLSSIVYL+AGHPEVEKKLLEEIDN+GPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV
Sbjct: 361 AFTLSSIVYLVAGHPEVEKKLLEEIDNYGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTV 420

Query: 510 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQR 569
           SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD FKPERFDP+ EEEKQR
Sbjct: 421 SPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDNFKPERFDPDAEEEKQR 480

Query: 570 HPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKN 629
           HPYALIPFGIGPRACIGQKFALQELKL+LIHLYRKF FRHSLSME PLELEYGVILNFKN
Sbjct: 481 HPYALIPFGIGPRACIGQKFALQELKLTLIHLYRKFAFRHSLSMEHPLELEYGVILNFKN 531

Query: 630 GVKLRVINRK 640
           GVKLRVINRK
Sbjct: 541 GVKLRVINRK 531

BLAST of Lsi04G014190 vs. NCBI nr
Match: TYK20085.1 (cytochrome P450 711A1 [Cucumis melo var. makuwa])

HSP 1 Score: 1003.8 bits (2594), Expect = 6.6e-289
Identity = 508/551 (92.20%), Postives = 519/551 (94.19%), Query Frame = 0

Query: 90  MDDFLQSVLASVSMGVGAILFTTLCVVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 149
           MD+  QS++ASV MGVG ILFTT C+VGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA
Sbjct: 1   MDNLFQSMVASVPMGVGTILFTTFCLVGGGLVYFYGPYWGVRRVPGPPAIPLVGHLPLLA 60

Query: 150 KYGPDVFSVLASQYGPIF-RFHMGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISAS 209
           KYGPDVFSVLASQYGPIF RFHMGRQPLIIIADPELCKEVGIKKFKDI NRSVPSPISAS
Sbjct: 61  KYGPDVFSVLASQYGPIFSRFHMGRQPLIIIADPELCKEVGIKKFKDISNRSVPSPISAS 120

Query: 210 PLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSII 269
           PLHQKGLFFT                  RDARWSTMRNTILSVYQPSHLT LIPTMQSII
Sbjct: 121 PLHQKGLFFT------------------RDARWSTMRNTILSVYQPSHLTRLIPTMQSII 180

Query: 270 ETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTD 329
           ETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNP ATKTCGTN   DSK D
Sbjct: 181 ETATQNLHSSVEEDIPFSNLSLKLTTDVIGTAAFGVNFGLSNPHATKTCGTN-DQDSKND 240

Query: 330 EVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQK 389
           EVSDFINQHIYSTTQLKMDLSGSFSIILGLLVP+LQEPFRQ+LKRIPFTMDWKVDRTNQK
Sbjct: 241 EVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPVLQEPFRQVLKRIPFTMDWKVDRTNQK 300

Query: 390 LSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSAT 449
           LSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSAT
Sbjct: 301 LSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSAT 360

Query: 450 TAFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYT 509
           TAFTLSS+VYL+AGHPEVEKKLLEEIDNFGPSDQIPTANDLQQ FPYLDQVIKE+MRFYT
Sbjct: 361 TAFTLSSVVYLVAGHPEVEKKLLEEIDNFGPSDQIPTANDLQQMFPYLDQVIKESMRFYT 420

Query: 510 VSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQ 569
           VSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPD+FKPERFDPNEEEEK+
Sbjct: 421 VSPLVARETSKDVEIGGYLLPKGTWVWLALGVLAKDPKNFPEPDKFKPERFDPNEEEEKR 480

Query: 570 RHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK 629
           RHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK
Sbjct: 481 RHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFK 532

Query: 630 NGVKLRVINRK 640
           NGVKLRVINRK
Sbjct: 541 NGVKLRVINRK 532

BLAST of Lsi04G014190 vs. TAIR 10
Match: AT2G26170.1 (cytochrome P450, family 711, subfamily A, polypeptide 1 )

HSP 1 Score: 761.9 bits (1966), Expect = 4.1e-220
Identity = 382/519 (73.60%), Postives = 433/519 (83.43%), Query Frame = 0

Query: 120 LVYFYGPYWGVRRVPGPPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIII 179
           ++Y Y P W V  VPGP A+PLVGHLPL+AKYGPDVFSVLA QYGPIFRF MGRQPLIII
Sbjct: 33  VIYLYRPSWSVCNVPGPTAMPLVGHLPLMAKYGPDVFSVLAKQYGPIFRFQMGRQPLIII 92

Query: 180 ADPELCKEVGIKKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDA 239
           A+ ELC+EVGIKKFKD+PNRS+PSPISASPLH+KGLFFT                  RD 
Sbjct: 93  AEAELCREVGIKKFKDLPNRSIPSPISASPLHKKGLFFT------------------RDK 152

Query: 240 RWSTMRNTILSVYQPSHLTSLIPTMQSIIETATQNLHSSVEEDIPFSNLSLKLTTDVIGT 299
           RWS MRNTILS+YQPSHLTSLIPTM S I +AT NL S    DI FSNL LKLTTD+IG 
Sbjct: 153 RWSKMRNTILSLYQPSHLTSLIPTMHSFITSATHNLDSK-PRDIVFSNLFLKLTTDIIGQ 212

Query: 300 AAFGVNFGLSNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSIILGLL 359
           AAFGV+FGLS           G    K  EV+DFINQH+YSTTQLKMDLSGS SIILGLL
Sbjct: 213 AAFGVDFGLS-----------GKKPIKDVEVTDFINQHVYSTTQLKMDLSGSLSIILGLL 272

Query: 360 VPILQEPFRQILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGSKDFLSLILRAR 419
           +PILQEPFRQ+LKRIP TMDW+V++TN +LSG+LNEIV KR K  +  SKDFLSLIL+AR
Sbjct: 273 IPILQEPFRQVLKRIPGTMDWRVEKTNARLSGQLNEIVSKRAKEAETDSKDFLSLILKAR 332

Query: 420 ESETVSRNVFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEIDNFGP 479
           ES+  ++N+FT DYISAVTYEHLLAGSATTAFTLSS++YL++GH +VEK+LL+EID FG 
Sbjct: 333 ESDPFAKNIFTSDYISAVTYEHLLAGSATTAFTLSSVLYLVSGHLDVEKRLLQEIDGFGN 392

Query: 480 SDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLVARETSKDVEIGGYLLPKGTWVWLALG 539
            D IPTA+DLQ KFPYLDQVIKEAMRFY VSPLVARET+K+VEIGGYLLPKGTWVWLALG
Sbjct: 393 RDLIPTAHDLQHKFPYLDQVIKEAMRFYMVSPLVARETAKEVEIGGYLLPKGTWVWLALG 452

Query: 540 VLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFALQELKLSLI 599
           VLAKDPKNFPEP++FKPERFDPN EEEK RHPYA IPFGIGPRAC+GQ+FALQE+KL+L+
Sbjct: 453 VLAKDPKNFPEPEKFKPERFDPNGEEEKHRHPYAFIPFGIGPRACVGQRFALQEIKLTLL 512

Query: 600 HLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKLRVINR 639
           HLYR ++FRHSL ME PL+L+YG+IL+FKNGVKLR I R
Sbjct: 513 HLYRNYIFRHSLEMEIPLQLDYGIILSFKNGVKLRTIKR 521

BLAST of Lsi04G014190 vs. TAIR 10
Match: AT2G26170.2 (cytochrome P450, family 711, subfamily A, polypeptide 1 )

HSP 1 Score: 679.9 bits (1753), Expect = 2.0e-195
Identity = 344/468 (73.50%), Postives = 391/468 (83.55%), Query Frame = 0

Query: 171 MGRQPLIIIADPELCKEVGIKKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLIN 230
           MGRQPLIIIA+ ELC+EVGIKKFKD+PNRS+PSPISASPLH+KGLFFT            
Sbjct: 1   MGRQPLIIIAEAELCREVGIKKFKDLPNRSIPSPISASPLHKKGLFFT------------ 60

Query: 231 ASIQFYRDARWSTMRNTILSVYQPSHLTSLIPTMQSIIETATQNLHSSVEEDIPFSNLSL 290
                 RD RWS MRNTILS+YQPSHLTSLIPTM S I +AT NL S    DI FSNL L
Sbjct: 61  ------RDKRWSKMRNTILSLYQPSHLTSLIPTMHSFITSATHNLDSK-PRDIVFSNLFL 120

Query: 291 KLTTDVIGTAAFGVNFGLSNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSG 350
           KLTTD+IG AAFGV+FGLS           G    K  EV+DFINQH+YSTTQLKMDLSG
Sbjct: 121 KLTTDIIGQAAFGVDFGLS-----------GKKPIKDVEVTDFINQHVYSTTQLKMDLSG 180

Query: 351 SFSIILGLLVPILQEPFRQILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGSKD 410
           S SIILGLL+PILQEPFRQ+LKRIP TMDW+V++TN +LSG+LNEIV KR K  +  SKD
Sbjct: 181 SLSIILGLLIPILQEPFRQVLKRIPGTMDWRVEKTNARLSGQLNEIVSKRAKEAETDSKD 240

Query: 411 FLSLILRARESETVSRNVFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKL 470
           FLSLIL+ARES+  ++N+FT DYISAVTYEHLLAGSATTAFTLSS++YL++GH +VEK+L
Sbjct: 241 FLSLILKARESDPFAKNIFTSDYISAVTYEHLLAGSATTAFTLSSVLYLVSGHLDVEKRL 300

Query: 471 LEEIDNFGPSDQIPTANDLQQKFPYLDQVIKEAMRFYTVSPLVARETSKDVEIGGYLLPK 530
           L+EID FG  D IPTA+DLQ KFPYLDQVIKEAMRFY VSPLVARET+K+VEIGGYLLPK
Sbjct: 301 LQEIDGFGNRDLIPTAHDLQHKFPYLDQVIKEAMRFYMVSPLVARETAKEVEIGGYLLPK 360

Query: 531 GTWVWLALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFA 590
           GTWVWLALGVLAKDPKNFPEP++FKPERFDPN EEEK RHPYA IPFGIGPRAC+GQ+FA
Sbjct: 361 GTWVWLALGVLAKDPKNFPEPEKFKPERFDPNGEEEKHRHPYAFIPFGIGPRACVGQRFA 420

Query: 591 LQELKLSLIHLYRKFVFRHSLSMEQPLELEYGVILNFKNGVKLRVINR 639
           LQE+KL+L+HLYR ++FRHSL ME PL+L+YG+IL+FKNGVKLR I R
Sbjct: 421 LQEIKLTLLHLYRNYIFRHSLEMEIPLQLDYGIILSFKNGVKLRTIKR 438

BLAST of Lsi04G014190 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-31
Identity = 125/512 (24.41%), Postives = 220/512 (42.97%), Query Frame = 0

Query: 131 RRVPGPPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGI 190
           R  P PP  P++G+L  L +        L+ +YGP+    +GR P +I++  E  K+   
Sbjct: 30  RTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYGPVMLLKLGRVPTVIVSSSETAKQA-- 89

Query: 191 KKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRN-TIL 250
            K  D+   S P              F    +  Y  L  A   +  D  W  +R   + 
Sbjct: 90  LKIHDLHCCSRPG-------------FAGARELSYNYLDIAFSPY--DDYWKEVRKLAVQ 149

Query: 251 SVYQPSHLTSLIPTMQSIIETATQNLHSSVEEDIP--FSNLSLKLTTDVIGTAAFGVNFG 310
            ++    + S+ P     ++    ++  S  +  P   +   L LT  V+   AF VNF 
Sbjct: 150 ELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRTAFSVNF- 209

Query: 311 LSNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSI-----ILGLLVPI 370
                                E +   ++   +  +  +++ GSFS       +G ++ +
Sbjct: 210 ---------------------EGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDL 269

Query: 371 LQEPFRQILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGSKDFLSLILRARESE 430
           L              +  + +R+ + L     ++ D   +  + GS+DF+ L+LR  + E
Sbjct: 270 LT------------GLQGRRERSMRDLDAFYEQMFDLHKQKKEEGSEDFVDLLLRLEKEE 329

Query: 431 TVSRN-VFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSD 490
            V  N   T ++I A+  + LLAG  T+A T++  +  +A +P V KK+  EI +   + 
Sbjct: 330 AVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNK 389

Query: 491 QIPTANDLQQKFPYLDQVIKEAMRFYTVSP-LVARETSKDVEIGGYLLPKGTWVWLALGV 550
           +  + +D   K  YL  VIKE  R +  +P L+ RE   + EI GY +P  T + + +  
Sbjct: 390 ERISFDD-TDKLEYLKMVIKETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWA 449

Query: 551 LAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFALQELKLSLIH 610
           + +DP  + +P+ F PERF  N  + K +H + L+PFG G R C         ++  L +
Sbjct: 450 IGRDPDTWKDPEVFLPERFTDNNIDAKGQH-FELLPFGGGRRMCPAVYMGTTMVEFGLAN 488

Query: 611 LYRKFVFRHSLSM---EQPLELEYGVILNFKN 630
           L   F ++    M   +  +E   G+ +N KN
Sbjct: 510 LLYHFDWKLPEGMKVDDIDMEEAPGLTVNKKN 488

BLAST of Lsi04G014190 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 132.9 bits (333), Expect = 9.2e-31
Identity = 119/511 (23.29%), Postives = 227/511 (44.42%), Query Frame = 0

Query: 131 RRVPGPPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVGI 190
           R+ P PP  P++G+L  + +        L+ +YGP+    +GR P ++++  +  ++V  
Sbjct: 29  RQFPCPPGFPIIGNLHQIGELPHQTLWKLSKKYGPVMHLMLGRVPTVVVSSSDTARQV-- 88

Query: 191 KKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRNTILS 250
            +  D+   + PS                G + L    ++ +   Y D      +  +  
Sbjct: 89  LRVHDLHCCTRPS--------------LSGPRELSYNYLDIAFSPYDDYWKEVRKLCVQE 148

Query: 251 VYQPSHLTSLIPTMQSIIETATQNLHSSVEEDIP--FSNLSLKLTTDVIGTAAFGVNFGL 310
           ++    + S+ P     ++    ++  S  +  P   +N  L+LT  V+   AFGV+F  
Sbjct: 149 LFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRTAFGVSF-- 208

Query: 311 SNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGS-FSIILGLLVPILQEPF 370
                             T   SD  N+ +    ++    S + F   +G ++ +L    
Sbjct: 209 ----------------EGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLT--- 268

Query: 371 RQILKRIPFTMDWKVDRTNQKLSGRLNEIVDKRMKCNDRGSKDFLSLILRARESETVSRN 430
                     +  + +R+ + L+    ++ D   +    G++DF+ L+LR  + E V  N
Sbjct: 269 ---------GLQGRRERSKRDLNAFFEQMFDLHKEGKKEGNEDFVDLLLRLEKEEAVLGN 328

Query: 431 -VFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEIDNFGPSDQIPTA 490
              T ++I A+  + LLAG  T+A T++  +  +A +P V KK+  EI     +  + + 
Sbjct: 329 DKLTRNHIKAILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISF 388

Query: 491 NDLQQKFPYLDQVIKEAMRFYTVSP-LVARETSKDVEIGGYLLPKGTWVWLALGVLAKDP 550
            D+ Q   YL  VIKE  R +  +P L+ RE   + +I GY +P  T + + +  + +DP
Sbjct: 389 EDMDQ-LEYLKMVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDP 448

Query: 551 KNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFALQELKLSLIHLYRKF 610
             + +P+ F PERF  N  + K +H + L+PFG G R C         ++  L +L   F
Sbjct: 449 DTWKDPEVFLPERFMDNNIDAKGQH-FELLPFGGGRRICPAIYMGTTMVEFGLANLLYHF 491

Query: 611 VFRHSLSME-QPLELEY--GVILNFKNGVKL 634
            ++    +E + +++E   G+ +N KN + L
Sbjct: 509 DWKLPEGVEVKDIDVEEAPGLTVNKKNELLL 491

BLAST of Lsi04G014190 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 131.7 bits (330), Expect = 2.1e-30
Identity = 127/490 (25.92%), Postives = 219/490 (44.69%), Query Frame = 0

Query: 130 VRRVPGPPAIPLVGHLPLLAKYGPDVFSVLASQYGPIFRFHMGRQPLIIIADPELCKEVG 189
           VR+ P PP +P++G+L  L +        L+ +YGP+    +GR P +I++ PE  K+V 
Sbjct: 28  VRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKKYGPVMLLKLGRVPTVIVSTPETAKQV- 87

Query: 190 IKKFKDIPNRSVPSPISASPLHQKGLFFTRGEKTLYQLLINASIQFYRDARWSTMRN-TI 249
             K  D+   S PS           L  TR  K  Y  L  A  +F  D  W  +R   +
Sbjct: 88  -LKDYDLHCCSRPS-----------LEGTR--KLSYNYLDIAFSRF--DDYWKELRKLCV 147

Query: 250 LSVYQPSHLTSLIPTMQSIIETATQNLHSSVEED--IPFSNLSLKLTTDVIGTAAFGVNF 309
             ++    + S+ P  ++ +E    ++  S  +   +  S+  L L  +VI  A FGVNF
Sbjct: 148 EELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNVICKAVFGVNF 207

Query: 310 GLSNPQATKTCGTNGHHDSKTDEVSDFINQHIYSTTQLKMDLSGSFSIILGLLVPILQEP 369
                Q T             D+  D +++         +++ GSFS             
Sbjct: 208 -----QGTVL---------NNDKFQDLVHE--------ALEMLGSFSA------------ 267

Query: 370 FRQILKRIPFTMDW------KVDRTNQKLSGRLNEIVDKRMKCN-DRGSKDFLSLILRAR 429
                  + + +DW      + +R+ + L     +++D  ++ N +    DF+ L+LR  
Sbjct: 268 -SDFFPYVGWIVDWFTGLHARRERSVRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRLE 327

Query: 430 ESETV-SRNVFTPDYISAVTYEHLLAGSATTAFTLSSIVYLIAGHPEVEKKLLEEI-DNF 489
           + E V      T ++I A+    LL G  T+A T++  +  +  +P V KK+  EI    
Sbjct: 328 KEEAVLGYGKLTRNHIKAILMNILLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQI 387

Query: 490 GPSDQIPTAN-DLQQKFPYLDQVIKEAMRFYTVSP-LVARETSKDVEIGGYLLPKGTWVW 549
           G +++    + D      YL+ VIKE  R + V+P LV RE   + +I GY +   T + 
Sbjct: 388 GKNNKTRIISLDEINHLSYLNMVIKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLH 447

Query: 550 LALGVLAKDPKNFPEPDQFKPERFDPNEEEEKQRHPYALIPFGIGPRACIGQKFALQELK 606
           + +  + +DP+ + +P++F PERF   + + K +  Y L+PFG G R C      +  ++
Sbjct: 448 VNVWAIGRDPEIWKDPEEFLPERFMDCDIDVKGQ-DYELLPFGSGRRICPAVYMGITTVE 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DFU25.7e-21973.60Cytochrome P450 711A1 OS=Arabidopsis thaliana OX=3702 GN=CYP711A1 PE=2 SV=1[more]
P477879.3e-5227.54Thromboxane-A synthase OS=Sus scrofa OX=9823 GN=TBXAS1 PE=1 SV=1[more]
Q2KIG55.1e-5026.97Thromboxane-A synthase OS=Bos taurus OX=9913 GN=TBXAS1 PE=2 SV=1[more]
P494308.7e-5028.55Thromboxane-A synthase OS=Rattus norvegicus OX=10116 GN=Tbxas1 PE=2 SV=1[more]
P245575.6e-4927.04Thromboxane-A synthase OS=Homo sapiens OX=9606 GN=TBXAS1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1F8E65.8e-29192.73cytochrome P450 711A1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443047 P... [more]
A0A1S3BUH61.3e-29092.36cytochrome P450 711A1 OS=Cucumis melo OX=3656 GN=LOC103493668 PE=3 SV=1[more]
A0A6J1IH852.2e-29092.36cytochrome P450 711A1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477201 PE=... [more]
A0A5D3D9543.2e-28992.20Cytochrome P450 711A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13... [more]
A0A0A0L0Q63.5e-28891.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646170 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023536371.15.4e-29192.73cytochrome P450 711A1 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022936422.11.2e-29092.73cytochrome P450 711A1 isoform X1 [Cucurbita moschata] >KAG7024349.1 Cytochrome P... [more]
XP_008452735.12.7e-29092.36PREDICTED: cytochrome P450 711A1 [Cucumis melo][more]
XP_022977002.14.6e-29092.36cytochrome P450 711A1 isoform X1 [Cucurbita maxima][more]
TYK20085.16.6e-28992.20cytochrome P450 711A1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G26170.14.1e-22073.60cytochrome P450, family 711, subfamily A, polypeptide 1 [more]
AT2G26170.22.0e-19573.50cytochrome P450, family 711, subfamily A, polypeptide 1 [more]
AT3G26300.11.1e-3124.41cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT3G26310.19.2e-3123.29cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT5G57260.12.1e-3025.92cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 575..584
score: 60.05
coord: 498..509
score: 35.48
coord: 584..595
score: 44.77
coord: 444..461
score: 35.74
IPR001128Cytochrome P450PFAMPF00067p450coord: 232..624
e-value: 2.5E-72
score: 244.1
coord: 134..222
e-value: 2.6E-11
score: 43.0
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 292..310
score: 22.29
coord: 537..561
score: 34.78
coord: 584..607
score: 32.33
coord: 497..515
score: 29.45
coord: 574..584
score: 52.82
coord: 160..179
score: 28.39
coord: 433..450
score: 24.36
coord: 453..479
score: 29.58
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 124..639
e-value: 6.1E-113
score: 379.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 126..638
NoneNo IPR availablePANTHERPTHR24301THROMBOXANE-A SYNTHASEcoord: 104..638
NoneNo IPR availablePANTHERPTHR24301:SF10CYTOCHROME P450 FAMILY PROTEINcoord: 104..638
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 577..586

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G014190.1Lsi04G014190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen