Lsi04G006510 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G006510
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	mitotic spindle checkpoint protein MAD2
Location	chr04: 5950735 .. 5953609 (-)
RNA-Seq Expression	Lsi04G006510
Synteny	Lsi04G006510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAGACGAACCCTTCATCTTTTACTTTTAGTCGCGTCCTGCATTTCCAAGTTCTCAAATTTTCTCAGATCCACTCGTTTTTCTCTTCGCCCATTTGATTAATTGATTCATTCAGTCCGACTTCTCTTTCAAATTTGCTTTCCCCTGTATCGATTAGAAACCCTAAATCCGCCCAGTCCAAGAAATTTTGGGGATTTTTTTCAGGTTGAAGATTTGGTATGGCTTCCAAAACAGCAACCAAAGATATCATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGTATTTTATGCTTCATTGCCTTCCGAAACCCTTTTAATTCGGTTGATTATCGATGAATAACCTTGGCTGATTTGTTCTTTTTCGTTTTCTTGAAATTTCTCATTTAGGGTATGCTGCAAATAGGTATGAATACTGGATATGCTTTCTCTGTTCTTATTTTTGTTATGGGATCGTGTAAATTTGCATTTTGTCACTTACAAGCGATGTTGATTGTAACTGTGTAGCATCCTTTACAATCGTGGGCTTTATCCGGAAGAAAGTTTTGTTAGGGTTAAGAAATATGGACTTCCTATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGGTTAGTTCCCTTTCTCTAAATGAAAGATTTGTAATTTCCAACAGTTCAGTATTGTATTTTTCTTTTTTCTCTTGCTGTAGTCCAGATTGTAGTGTGATTTATACTTTCCTGAGAATTATATTTATTGAGAAGTTTTGGAATCGTGAAATTGGTTTTAGAATGGCTGGAAGCTGGGAAGTTACAAAGGGTTGTGCTAGTTATAATGAGCAAATCCAACAATGAGGTCCTTGAGAGGTGGAATTTCAGAATCGAAACTGACGGAGAGGTGGTCGAGCAAGGGTATAACAAATTCAACGAAAGATTTCTCTTCCCCTTTCCTAAATTTTCTGGATTTTGTGTTGATTAGATGACATTGTTGCAGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACCTACTTGCCCTGCCTAGATGAACCCTGTAAGTGTTGGCAAAATCTTATATTTTAGTGATTGGTGTAAACATAATGCGATATTGTTTTAGCCGGGTTCTGAGTCCATGAGATGTCTAGTTTCCATTATGAAATTCATTATGCACAACCCTGTAAGTTTTGCTTCAATCATCACTTGCCTGCCTGATCCTTTGTTATTTTGGGGATTGGTTTGACGTGTGAAGGCTTATGTTTTTAGGCTGAGTTATGAATCAGTTGGATGCCATATAAGTTCCAGCATGAAATCATTTCCTTTAATAGCTGATTTGAACATTGCTTGAATGGCTAGGCATAGGCGTATCTTTCTTATGGGAATTAAGTTCAAATCAATACCCTTCTTTTATATTCTAAAAAGAAAATCATTAGTTTCTAATGATTTGCTACGGTCTTAGATGATAATCTAGATGTTTGAGCTCTTTTTCTTTTAGACGATACGATCCATCATGCTCAATCAAGTGTTACTCTGATGTTTTTGTTGTCTCAGGTGTATTTGATGTGTTAGCATATACTGATAAGGATCTTGCAGTTCCATTTACTTGGATGGAGAGTGATCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCTTTTGACACAAAGGTGAGGGAATGGGACTTTCGTGCAGGAATATCGAACTTATTTGTTTCCACTAATCTCGATTCGCAGCAAGTAAATTAACTTTTGAATGGTGGTGTCATTGCAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAATGATGATTGCGATGACGAGTAAAAAACAAAACAAACTCCCTTTTCTTCAATCTGTTGTCGGTTTCAGAATCCTCCAACCCAATCTCGCTTTCAAGGTGGCATTGTGAATCATTTATTCACAATTTTTATTGTACTATAACTTAGGAACAATGTTGTTGTGGATTCGATTAGAATTCTACCTGAATGAAATGGTTGAGGATTGTTTCTTTTTAAAGAAAACATATTTCTAATTCTACCTGAATGAAATGGTTGAGGATTGTTTCTTTTTAAAGAAAACATATTTCTACGGATAAGGTTGTTGTGTCTTTTAAGAACAAGAGTGGACCAAGTCTTGATCCACTCAGTTTAGCCAAATTCTTCGTTCAAACCAAAGATTTTTGTTCTCTAAATATAACAACCATCCATAATTAGAAACTTCCAAGTAAATTTATCTTGAAAAATCGAAAATTTTGATGAAGCCAAGAGAAGTTGTTGAGAAAAAAGAAAAAGGAAAAAGAAGAATGGGTTTAGATGGTGGTCTGTCTTGGGCAGATCAATGGGATAACAACCCTGACCCTCCACCACCATCTTCATCGGAAAATGAGAAGAAGAAGAACAAGGACGGGTCCTCAGACAAGAGCAAATTTAGGAAGACAATCTTGGGCTTCAAATGGATGAAAGAATTGCGAAAGAAATCTAACAAATCTTGAAAAACAGGCCCATAAAATCCCAATCGAAGTGAAATGAATCACTGTGTTTTCCCATTTTTATTTGTACTTTCTTCCCTTTTTGTTGAGCTCTGAATGAAAGAAGCCAGAAAATGAGAGCATAAAAATTCTGGTTTTCCCAAAACTCAGCATCCAATGAATTCTAATATATTATTACATTACAAGATTATGCTTCTTCTGTTCTTTTCTTCTTCTTCCAAACAAATTGTAGGGGTCGAGGACAAAAAGCTTCAGGTTTTTGAAAGATAATAGATGTCTTGTTCACAAACTATTTTTATATCATACATCTGAAATATGTCCAAA

mRNA sequence

AGGAGACGAACCCTTCATCTTTTACTTTTAGTCGCGTCCTGCATTTCCAAGTTCTCAAATTTTCTCAGATCCACTCGTTTTTCTCTTCGCCCATTTGATTAATTGATTCATTCAGTCCGACTTCTCTTTCAAATTTGCTTTCCCCTGTATCGATTAGAAACCCTAAATCCGCCCAGTCCAAGAAATTTTGGGGATTTTTTTCAGGTTGAAGATTTGGTATGGCTTCCAAAACAGCAACCAAAGATATCATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGGTATGCTGCAAATAGGTATGAATACTGGATATGCTTTCTCTGTTCTTATTTTTGTTATGGGATCGTCATCCTTTACAATCGTGGGCTTTATCCGGAAGAAAGTTTTGTTAGGGTTAAGAAATATGGACTTCCTATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGATTGTAGTGTGATTTATACTTTCCTGAGAATTATATTTATTGAGAAGTTTTGGAATCGTGAAATTGAATGGCTGGAAGCTGGGAAGTTACAAAGGGTTGTGCTAGTTATAATGAGCAAATCCAACAATGAGGTCCTTGAGAGGTGGAATTTCAGAATCGAAACTGACGGAGAGGTGGTCGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACCTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATATACTGATAAGGATCTTGCAGTTCCATTTACTTGGATGGAGAGTGATCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCTTTTGACACAAAGGTGAGGGAATGGGACTTTCGTGCAGGAATATCGAACTTATTTGTTTCCACTAATCTCGATTCGCAGCAAATTCACAAAGTGGACACTCTTGTTTCTTACAAGAATGATGATTGCGATGACGAGTAAAAAACAAAACAAACTCCCTTTTCTTCAATCTGTTGTCGGTTTCAGAATCCTCCAACCCAATCTCGCTTTCAAGGTGGCATTGTGAATCATTTATTCACAATTTTTATTGTACTATAACTTAGGAACAATGTTGTTGTGGATTCGATTAGAATTCTACCTGAATGAAATGGTTGAGGATTGTTTCTTTTTAAAGAAAACATATTTCTACGGATAAGGTTGTTGTGTCTTTTAAGAACAAGAGTGGACCAAGTCTTGATCCACTCAGTTTAGCCAAATTCTTCGTTCAAACCAAAGATTTTTGTTCTCTAAATATAACAACCATCCATAATTAGAAACTTCCAAGTAAATTTATCTTGAAAAATCGAAAATTTTGATGAAGCCAAGAGAAGTTGTTGAGAAAAAAGAAAAAGGAAAAAGAAGAATGGGTTTAGATGGTGGTCTGTCTTGGGCAGATCAATGGGATAACAACCCTGACCCTCCACCACCATCTTCATCGGAAAATGAGAAGAAGAAGAACAAGGACGGGTCCTCAGACAAGAGCAAATTTAGGAAGACAATCTTGGGCTTCAAATGGATGAAAGAATTGCGAAAGAAATCTAACAAATCTTGAAAAACAGGCCCATAAAATCCCAATCGAAGTGAAATGAATCACTGTGTTTTCCCATTTTTATTTGTACTTTCTTCCCTTTTTGTTGAGCTCTGAATGAAAGAAGCCAGAAAATGAGAGCATAAAAATTCTGGTTTTCCCAAAACTCAGCATCCAATGAATTCTAATATATTATTACATTACAAGATTATGCTTCTTCTGTTCTTTTCTTCTTCTTCCAAACAAATTGTAGGGGTCGAGGACAAAAAGCTTCAGGTTTTTGAAAGATAATAGATGTCTTGTTCACAAACTATTTTTATATCATACATCTGAAATATGTCCAAA

Coding sequence (CDS)

ATGGCTTCCAAAACAGCAACCAAAGATATCATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGGTATGCTGCAAATAGGTATGAATACTGGATATGCTTTCTCTGTTCTTATTTTTGTTATGGGATCGTCATCCTTTACAATCGTGGGCTTTATCCGGAAGAAAGTTTTGTTAGGGTTAAGAAATATGGACTTCCTATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGATTGTAGTGTGATTTATACTTTCCTGAGAATTATATTTATTGAGAAGTTTTGGAATCGTGAAATTGAATGGCTGGAAGCTGGGAAGTTACAAAGGGTTGTGCTAGTTATAATGAGCAAATCCAACAATGAGGTCCTTGAGAGGTGGAATTTCAGAATCGAAACTGACGGAGAGGTGGTCGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACCTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATATACTGATAAGGATCTTGCAGTTCCATTTACTTGGATGGAGAGTGATCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCTTTTGACACAAAGGTGAGGGAATGGGACTTTCGTGCAGGAATATCGAACTTATTTGTTTCCACTAATCTCGATTCGCAGCAAATTCACAAAGTGGACACTCTTGTTTCTTACAAGAATGATGATTGCGATGACGAGTAA

Protein sequence

MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISNLFVSTNLDSQQIHKVDTLVSYKNDDCDDE

Homology

[Close]

BLAST of Lsi04G006510 vs. ExPASy Swiss-Prot
Match: Q9LU93 (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.2e-85
Identity = 177/270 (65.56%), Postives = 193/270 (71.48%), Query Frame = 0


Query: 1   MASKT-ATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEES 60
           MASKT A KDIITL GSAAIVSEFF YAAN                  ILYNR +YPEES
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANS-----------------ILYNRAVYPEES 60

Query: 61  FVRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGK 120
           FV+VKKYGLPMLL +DE VK+F+SNLT+Q+S                      EWLEAGK
Sbjct: 61  FVKVKKYGLPMLLIEDESVKSFMSNLTSQIS----------------------EWLEAGK 120

Query: 121 LQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSIT 180
           LQRVVLVIMSK+  EVLERWNFRIETD EVV++GVSREKSDKEIMREIQAIMRQ+ASS+T
Sbjct: 121 LQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVT 180

Query: 181 YLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGIS 240
           YLPCLDE CVFDVLAYTD D+AVPFTW+ESDPKLIANPQMVKLH FDTK           
Sbjct: 181 YLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTK----------- 208

Query: 241 NLFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                       IHKVDTLVSYKND+ D+E
Sbjct: 241 ------------IHKVDTLVSYKNDEWDEE 208

[Close]

BLAST of Lsi04G006510 vs. ExPASy Swiss-Prot
Match: Q9XFH3 (Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.4e-81
Identity = 170/269 (63.20%), Postives = 187/269 (69.52%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MAS++A+KDIITLRGSAAIVSEFFGYAAN                  ILYNR +YPEESF
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRAVYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            +VKKYGL MLLTQDEGVK FI++LT+QLS                      EWLEAGKL
Sbjct: 61  SKVKKYGLTMLLTQDEGVKNFIASLTSQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QR+VLVIMSK+ +EVLERWNF I TD EVVE+G  +EKSDKEIMREIQAIMRQIAS ITY
Sbjct: 121 QRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKSDKEIMREIQAIMRQIASCITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTD D+  P TW+ESD KLI NPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYK D+ D+E
Sbjct: 241 -----------IHKVDTLVSYKKDEWDEE 207

[Close]

BLAST of Lsi04G006510 vs. ExPASy Swiss-Prot
Match: Q556Y9 (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX=44689 GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 7.1e-46
Identity = 111/263 (42.21%), Postives = 144/263 (54.75%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           M +  A+K  I+L+GS  IV+EFF Y+ N                  IL+ RGLYP ESF
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSIN-----------------TILFQRGLYPPESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RV KYGLP+L+T D+ +K ++ N+  QLS                      EWL +G +
Sbjct: 61  TRVAKYGLPILVTNDQSLKDYLDNVLKQLS----------------------EWLLSGDV 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQG-VSREKSDKEIMREIQAIMRQIASSIT 180
           Q++VLVI      EVLERW F + TD  + ++G   R+K +KEIM EIQAI+RQI +S+T
Sbjct: 121 QKLVLVITDIVTKEVLERWVFDVTTD--IPKEGEAPRQKPEKEIMNEIQAIIRQITASVT 180

Query: 181 YLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGIS 240
           +LP L   C FD+L YT KDLAVP  W ESDPK I N Q VKL SF T            
Sbjct: 181 FLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQVKLRSFTT------------ 199

Query: 241 NLFVSTNLDSQQIHKVDTLVSYK 263
                       IHKV+++V+YK
Sbjct: 241 -----------TIHKVESMVAYK 199

[Close]

BLAST of Lsi04G006510 vs. ExPASy Swiss-Prot
Match: Q9Z1B5 (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 1.1e-41
Identity = 102/262 (38.93%), Postives = 139/262 (53.05%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MA + A +  ITLRGSA IV+EFF +  N                  ILY RG+YP E+F
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFFSFGINS-----------------ILYQRGIYPSETF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RV+KYGL +L T D  +  +++N+  QL                       EWL    +
Sbjct: 61  TRVQKYGLTLLTTTDPELIKYLNNVVEQLK----------------------EWLYKCSV 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           Q++V+VI +  + EVLERW F IE D    E+GV REKS K I  EI++++RQI +++T+
Sbjct: 121 QKLVVVISNIESGEVLERWQFDIECDKTAKEEGVRREKSQKAIQDEIRSVIRQITATVTF 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LP L+  C FD+L YTDKDL VP  W ES P+ I N + V+L SF T             
Sbjct: 181 LPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNCEEVRLRSFTT------------- 200

Query: 241 LFVSTNLDSQQIHKVDTLVSYK 263
                      IHKV+++V+YK
Sbjct: 241 ----------TIHKVNSMVAYK 200

[Close]

BLAST of Lsi04G006510 vs. ExPASy Swiss-Prot
Match: Q13257 (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.0e-39
Identity = 98/262 (37.40%), Postives = 137/262 (52.29%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MA + + +  ITLRGSA IV+EFF +  N                  ILY RG+YP E+F
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFFSFGINS-----------------ILYQRGIYPSETF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RV+KYGL +L+T D  +  +++N+  QL D                      WL    +
Sbjct: 61  TRVQKYGLTLLVTTDLELIKYLNNVVEQLKD----------------------WLYKCSV 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           Q++V+VI +  + EVLERW F IE D    +    REKS K I  EI++++RQI +++T+
Sbjct: 121 QKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTF 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LP L+  C FD+L YTDKDL VP  W ES P+ I N + V+L SF T             
Sbjct: 181 LPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTT------------- 200

Query: 241 LFVSTNLDSQQIHKVDTLVSYK 263
                      IHKV+++V+YK
Sbjct: 241 ----------TIHKVNSMVAYK 200

[Close]

BLAST of Lsi04G006510 vs. ExPASy TrEMBL
Match: A0A0A0KPY5 (HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.2e-98
Identity = 205/269 (76.21%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTATKDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
           VRVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDD DDE
Sbjct: 241 -----------IHKVDTLVSYKNDDDDDE 207

[Close]

BLAST of Lsi04G006510 vs. ExPASy TrEMBL
Match: A0A6J1HTK5 (mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466640 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.9e-98
Identity = 202/269 (75.09%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTA+KDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  ARVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSK+NNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDDCDDE
Sbjct: 241 -----------IHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of Lsi04G006510 vs. ExPASy TrEMBL
Match: A0A6J1GXP9 (mitotic spindle checkpoint protein MAD2 OS=Cucurbita moschata OX=3662 GN=LOC111458459 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.9e-98
Identity = 202/269 (75.09%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTA+KDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  ARVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSK+NNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDDCDDE
Sbjct: 241 -----------IHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of Lsi04G006510 vs. ExPASy TrEMBL
Match: A0A6J1KEW7 (mitotic spindle checkpoint protein MAD2-like OS=Cucurbita maxima OX=3661 GN=LOC111493236 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 3.2e-97
Identity = 201/269 (74.72%), Postives = 204/269 (75.84%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTA+KDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  ARVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSK+NNEVLERWNFRIETDG VVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDDCDDE
Sbjct: 241 -----------IHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of Lsi04G006510 vs. ExPASy TrEMBL
Match: A0A5A7STC2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002160 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 7.0e-97
Identity = 202/267 (75.66%), Postives = 203/267 (76.03%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTATKDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
           VRVKKYGLPMLLTQDEGVK+FISNLTAQLS                      EWLEAGKL
Sbjct: 61  VRVKKYGLPMLLTQDEGVKSFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK------------ 205

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCD 268
                      IHKVDTLVSYKNDD D
Sbjct: 241 -----------IHKVDTLVSYKNDDDD 205

[Close]

BLAST of Lsi04G006510 vs. NCBI nr
Match: KAE8648685.1 (hypothetical protein Csa_009142 [Cucumis sativus])

HSP 1 Score: 368.6 bits (945), Expect = 4.5e-98
Identity = 205/269 (76.21%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTATKDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 67  MASKTATKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 126

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
           VRVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 127 VRVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 186

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 187 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 246

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK            
Sbjct: 247 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK------------ 273

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDD DDE
Sbjct: 307 -----------IHKVDTLVSYKNDDDDDE 273

[Close]

BLAST of Lsi04G006510 vs. NCBI nr
Match: XP_004135301.1 (mitotic spindle checkpoint protein MAD2 [Cucumis sativus])

HSP 1 Score: 368.6 bits (945), Expect = 4.5e-98
Identity = 205/269 (76.21%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTATKDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
           VRVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDD DDE
Sbjct: 241 -----------IHKVDTLVSYKNDDDDDE 207

[Close]

BLAST of Lsi04G006510 vs. NCBI nr
Match: XP_022956917.1 (mitotic spindle checkpoint protein MAD2 [Cucurbita moschata] >XP_022967135.1 mitotic spindle checkpoint protein MAD2 [Cucurbita maxima] >XP_023534396.1 mitotic spindle checkpoint protein MAD2-like [Cucurbita pepo subsp. pepo] >KAG6601576.1 Mitotic spindle checkpoint protein MAD2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 367.5 bits (942), Expect = 1.0e-97
Identity = 202/269 (75.09%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTA+KDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  ARVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSK+NNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDDCDDE
Sbjct: 241 -----------IHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of Lsi04G006510 vs. NCBI nr
Match: XP_023542211.1 (mitotic spindle checkpoint protein MAD2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-97
Identity = 201/269 (74.72%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTA+KDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  ARVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSK+NNEVLERWNFRIETDGEVV+QGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKANNEVLERWNFRIETDGEVVDQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDDCDDE
Sbjct: 241 -----------IHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of Lsi04G006510 vs. NCBI nr
Match: KAG7012753.1 (Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-97
Identity = 201/269 (74.72%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEESF 60
           MASKTA+KDIITLRGSAAIVSEFFGYAAN                  ILYNRGLYPEESF
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANS-----------------ILYNRGLYPEESF 60

Query: 61  VRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGKL 120
            RVKKYGLPMLLTQDEGVKTFISNLTAQLS                      EWLEAGKL
Sbjct: 61  ARVKKYGLPMLLTQDEGVKTFISNLTAQLS----------------------EWLEAGKL 120

Query: 121 QRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180
           QRVVLVIMSK+NNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY
Sbjct: 121 QRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITY 180

Query: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGISN 240
           LPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK            
Sbjct: 181 LPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------ 207

Query: 241 LFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                      IHKVDTLVSYKNDDCDD+
Sbjct: 241 -----------IHKVDTLVSYKNDDCDDD 207

[Close]

BLAST of Lsi04G006510 vs. TAIR 10
Match: AT3G25980.1 (DNA-binding HORMA family protein )

HSP 1 Score: 317.8 bits (813), Expect = 8.5e-87
Identity = 177/270 (65.56%), Postives = 193/270 (71.48%), Query Frame = 0


Query: 1   MASKT-ATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEES 60
           MASKT A KDIITL GSAAIVSEFF YAAN                  ILYNR +YPEES
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANS-----------------ILYNRAVYPEES 60

Query: 61  FVRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGK 120
           FV+VKKYGLPMLL +DE VK+F+SNLT+Q+S                      EWLEAGK
Sbjct: 61  FVKVKKYGLPMLLIEDESVKSFMSNLTSQIS----------------------EWLEAGK 120

Query: 121 LQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSIT 180
           LQRVVLVIMSK+  EVLERWNFRIETD EVV++GVSREKSDKEIMREIQAIMRQ+ASS+T
Sbjct: 121 LQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVT 180

Query: 181 YLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGIS 240
           YLPCLDE CVFDVLAYTD D+AVPFTW+ESDPKLIANPQMVKLH FDTK           
Sbjct: 181 YLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTK----------- 208

Query: 241 NLFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                       IHKVDTLVSYKND+ D+E
Sbjct: 241 ------------IHKVDTLVSYKNDEWDEE 208

[Close]

BLAST of Lsi04G006510 vs. TAIR 10
Match: AT3G25980.2 (DNA-binding HORMA family protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.0e-84
Identity = 173/270 (64.07%), Postives = 189/270 (70.00%), Query Frame = 0


Query: 1   MASKT-ATKDIITLRGSAAIVSEFFGYAANRYEYWICFLCSYFCYGIVILYNRGLYPEES 60
           MASKT A KDIITL GSAAIVSEFF                       ILYNR +YPEES
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFF---------------------CTILYNRAVYPEES 60

Query: 61  FVRVKKYGLPMLLTQDEGVKTFISNLTAQLSDCSVIYTFLRIIFIEKFWNREIEWLEAGK 120
           FV+VKKYGLPMLL +DE VK+F+SNLT+Q+S                      EWLEAGK
Sbjct: 61  FVKVKKYGLPMLLIEDESVKSFMSNLTSQIS----------------------EWLEAGK 120

Query: 121 LQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSIT 180
           LQRVVLVIMSK+  EVLERWNFRIETD EVV++GVSREKSDKEIMREIQAIMRQ+ASS+T
Sbjct: 121 LQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVT 180

Query: 181 YLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKVREWDFRAGIS 240
           YLPCLDE CVFDVLAYTD D+AVPFTW+ESDPKLIANPQMVKLH FDTK           
Sbjct: 181 YLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTK----------- 204

Query: 241 NLFVSTNLDSQQIHKVDTLVSYKNDDCDDE 270
                       IHKVDTLVSYKND+ D+E
Sbjct: 241 ------------IHKVDTLVSYKNDEWDEE 204

The following BLAST results are available for this feature:

BLAST of Lsi04G006510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU93	1.2e-85	65.56	Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 ...	[more]
Q9XFH3	1.4e-81	63.20	Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1	[more]
Q556Y9	7.1e-46	42.21	Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX...	[more]
Q9Z1B5	1.1e-41	38.93	Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Ma...	[more]
Q13257	1.0e-39	37.40	Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD...	[more]

BLAST of Lsi04G006510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPY5	2.2e-98	76.21	HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 ...	[more]
A0A6J1HTK5	4.9e-98	75.09	mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466...	[more]
A0A6J1GXP9	4.9e-98	75.09	mitotic spindle checkpoint protein MAD2 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KEW7	3.2e-97	74.72	mitotic spindle checkpoint protein MAD2-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5A7STC2	7.0e-97	75.66	Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of Lsi04G006510 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648685.1	4.5e-98	76.21	hypothetical protein Csa_009142 [Cucumis sativus]	[more]
XP_004135301.1	4.5e-98	76.21	mitotic spindle checkpoint protein MAD2 [Cucumis sativus]	[more]
XP_022956917.1	1.0e-97	75.09	mitotic spindle checkpoint protein MAD2 [Cucurbita moschata] >XP_022967135.1 mit...	[more]
XP_023542211.1	2.2e-97	74.72	mitotic spindle checkpoint protein MAD2 [Cucurbita pepo subsp. pepo]	[more]
KAG7012753.1	2.2e-97	74.72	Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of Lsi04G006510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25980.1	8.5e-87	65.56	DNA-binding HORMA family protein	[more]
AT3G25980.2	4.0e-84	64.07	DNA-binding HORMA family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003511	HORMA domain	PFAM	PF02301	HORMA	coord: 48..227 e-value: 5.0E-12 score: 45.9
IPR003511	HORMA domain	PROSITE	PS50815	HORMA	coord: 31..236 score: 25.85745
IPR036570	HORMA domain superfamily	GENE3D	3.30.900.10	HORMA domain	coord: 1..236 e-value: 5.8E-63 score: 214.0
IPR036570	HORMA domain superfamily	SUPERFAMILY	56019	The spindle assembly checkpoint protein mad2	coord: 11..233
IPR027097	Mitotic spindle checkpoint protein Mad2	PANTHER	PTHR11842:SF11	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A	coord: 1..265
IPR045091	Mad2-like	PANTHER	PTHR11842	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2	coord: 1..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G006510.1	Lsi04G006510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000776	kinetochore
cellular_component	GO:0005654	nucleoplasm