Lsi04G005580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G005580
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionpolygalacturonase At1g48100-like
Locationchr04: 5091579 .. 5095870 (-)
RNA-Seq ExpressionLsi04G005580
SyntenyLsi04G005580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGGGATAAGTTTCAGAGGGCTCACATTATTTGTGTTTGTGTTGGCATTTCTTATCTGGTCTTCTAGTTTGGAGGGCTGCCATGGAAGAGCCACAAAGAAGCATTACTGGAGGAAGAACAGAGTGGCTGACGGTCACGGTGGCTCTCACAAAGGCAACCATCATGGTAGTGCATCAAAGACAAAGCCCTCGCCGTCTCATAAGACGCCGCAACCACTGCCACCACCGGTTACTAAGCTGAAACCAAATGTCCCTCGCAACCCGCCACCCCAGAAGGGGGGCCATTCTTCCATGTTTAACGTGCTGGATTTTGGAGCTAAAGGCGATGGAGAAACTGATGACACTAAGGTGAAACATTAAATCTTTATCTTCATAAAAGTAGGAGAAATTTTTCTATACTACATTTCCTTCCTTTATTGTTTGCTACGTGAATCTAATCGTAAGAATAGGGCGAGATTTACCTATATAAATGTAAAGTCCATTAATATCTACGAAGATAAACTTATAAGGTAAGCATAGAAAGTATAGTTAGGAAGTTACAATTACACATTATGAGTACGTGCGGTGGAAATGGTATTTTACGGGGAAGGGAAATGGAAATGGAAAAGGTGGGGTGGGCCCATCTAGAAAGGCAGAATCTCGTTATGGCAGTTGGGTCAGGATTTGAGTAAGAATAATTGGGTGGAGTAGGCAGCAACCTGAAAACTAACGCCAAGCAGTTAAAAAAGTCTGTTTTACCGTCAGTAAATAGAGGTAACAGTAAAAGAAAAAATGGTGTATATCGCAGGCATTTCAGGACACATGGACAGCCAGTTGCAAGGTGGCAGCATCAACCTTCATCGTCCCATCAAAATACGTCTTTTTAGTGGGCCCCATCTCCTTCTATGGTCCCTACTGCCAGCCCGATATCGTATTCCAGGTACCTAAACTACTAAGCACAAATTATTCCCACTTTAAATTTTACTTGTGTCAATTATTTCTTTTAAGTAGGAGTCATCATTGATCGGTCGATCAACGTCGGTTTTACATAAAAACTGTCGTTGACTATCGATGAGATGGATTTACATCGATCGTTCAGAGACAGTTTTGAAGTGAGTATGAGAGCGGTCGGTCGACGAAGCTTTATAAAAAAAATAGAGAAAAGGAAGAGCCAGATATAAGAAAAGAATAAGGGATAGAGAGAGAACAACAAACCAAAAAGAAATGGAGAATAGAGACCAAATGGAGAAAAGAAGAAAGAAGGTAGGAGAGAATAATAAATCGAAAATGGAAAAAATAAGAAAATAAATGAAAAAAAGGAAGAGGAAGAGGAAGAAGATGGATATATATACTTCGATAGAGAAAAAGTGGTTGAAATAGAAGGTAAAGACGTGTGATTGAGAGAAAAAAGGACAAAGATATTATTGTGAATTAATGGTGGAAAAGATAAAAAGAGAGAAATTCTTATTTTCTTTTTTTATAAACAGAGGTGATCATCAGTAGGTGGCATCGGTTTTGGGGCAAAACCTATCGTCGGCTACCGACAAGTCGGTTTCAAGCGGTTGGCTCTAATCGGTTTTGGCATGACTAAGGTTATCAAACGGCTGACAATACGGATGGACAATCGGTGTCGTCGGTTTAATAAGTCCAGTTAATTGAAAAGAAATAAAAACAAAAAAGAGATTAGAAAACATGAAGAAGAGAAAAGGAAAAAGGAAAAGAAAGAGAATTGAAGAAAAAAAAGTAAACAAGGAAGAACAAGAACACAAAATAATAGAGATTACGAAGAAACAAAATAAAGAAGAGAGGGAGAGGAAAGAAAGAGGATAGAGAAGATAAATGAAGAAAGAGGGAGAGGGTTTCATGAGATGGAGGGAAAAAAAGAGAGAGAAAAAGATGATGATGAACCTTCGGAGTTGGGGAAAGAGAAAAGAGAGTAACAAAATTATTATTTTATAAATTATAAGTGGTTTGTGAGAAAACCACTAGTACCCCATGGTTTTGAGCTTTGAATGTGATGTGACTACACATTTTTCACCATTTTTTTCACTCTCATCCCATCTATCCACTATTTTAAGATAATTTTATAATAAAAACATATATTATTTATATTATATAATATAATATATTTAAGAAAAAATATATAAATTAATATTCGGTCGGAATCGGTTTAATAGTCGGTTGGATGTGGTTGGTTTTTAACTGAATTTTGAAAACCCTACCTTCCGACCGGTTTTTTGGAAAAATAACCCTCCGACCTTGGGTTCATCGGTTTCAGTCGTTTGGCTCGGTTTTTCGGCTATCCTTGCTCACCCCTATTTGATGAAGAAGTGTTATATCACATCCAACAGTTCGATCGAGATTATGAATTTTATATCTTTCGACCAATCAAATTCTGGGTTTTCAAATTACAAAATCGACTTCGACCAGAAATTGCTTAAAATCGGCCTATCAATGACGGCTTGGTTGGTTTCCATCATTCTAATCGGTTTTTTCGGTTTGGTTTGCTCACCCCAACTTTATAGTCTTCAATTAATTTTATTACTAGTTCTAATCCCTTTCATGAATCATCCAACCAATTGACATGAACTTGACAATTTCTTGTTAAAATTACGTTTTCCATCAAAATTAATCTATTCATTATGAGACAACTAAATACCAAAATTACACTATGAAATAGTTAATAAATCTGCAACTATTTCTGTTTTTTTTTTCATTTTAAAAAAAGTTGGACGGGACAATCATTGCACCGACAGACTTCAAAGTTTGGGGCAAAGGTCTATTGCAATGGCTACAATTCACAAAGTTGATGGGACTTACGATTCAAGGAAATGGAATTATTGATGGAAGGGGTTCAGCATGGTGGCAAGTTTCACCTTATGATGATCCCATTGACGATGAATTCAAACTACTTATCCCACTCAACAACACTGTACAAGAAAAGCCACCCGTCCCGGTAACTCACCCTTCTTCAAACCCCACATTATGGTTTCTAAGAATTCTCTGCAACATTTTCTCTAAATTCATACTGAAACCTATGGCAGGTAAGAAGTGAGCTTGTGGGAAAGATGCCTAGGATCAAACCAACGGTAAGCGACAAACTAAATCTCTAAATTCCTTTATCTCGTTTCATTATTTGTGAAAAGATCCCTTGACGTGACCCAGTTCTTGTGTTGTACAGGCTTTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAATAGTCCTCAATGCCACCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTACTGTTTCATCTCCAGGTGATAGTCTCAATACTGACGGAATTCATTTGCAAAATTCCAAGGATGTGCTAATCTACAGCTCTACTCTCTCTTGTGGTATGAATTACTTTGCATAGAATCATTATTTTTGTCTTATCGTCATTTCGACCATAGTGGGACGTGACATCGTTACATGAAAGATTGAGACATGCATATACAAGAACCAATCAGTTCTAAAAACTAACTGCTAAACTTGATCTTACACATAGGTGATGACTGTATTTCAATACAAACGGGTTGCTCAAATGTGTATATACACAATGTCAATTGTGGACCAGGACATGGAATCAGCATTGGAAGTCTAGGGAAAGACAACACAAAGGCTTGTGTTTCTAACATCACTGTCCGTGATGTAATTATGCATAACACAATGAATGGTGTTAGAATTAAAACGTGGCAGGTATGCATTCCAAGCACAATTCACCTTTAAACTTAACTCATCAATGGTGCTAGTCTTGATCAGTTAAGTAAATGATTTATTTTGCATTAATTTGTTATCTTGAGGAAAATAAAAACCTATTAGCAATATGCACTATAAGATCAAACCAACTATCTCAAAACTTATTCCAAACTGTACATTTGCAGGGTGGATTAGGTTTTGTACAAGGAGTCCTCTTCTCAAATATTCAGGTTTCTGAGGTTAAGATACCAATAGTTATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTACACAGTAAAGCCAGTACACTTTGCCTGTAGTGATAATCTGCCATGTACAGATATATCACTAACTGCAATTGAGCTCAAGCCACTGCAAGAACTCTATCATTTATACGAGGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGAGTGTTTGCAAATTGGGAAGCCATCAAGCAACCAAATTCAGTATGATTTTGACTCTTGTTGA

mRNA sequence

ATGAAAGGGATAAGTTTCAGAGGGCTCACATTATTTGTGTTTGTGTTGGCATTTCTTATCTGGTCTTCTAGTTTGGAGGGCTGCCATGGAAGAGCCACAAAGAAGCATTACTGGAGGAAGAACAGAGTGGCTGACGGTCACGGTGGCTCTCACAAAGGCAACCATCATGGTAGTGCATCAAAGACAAAGCCCTCGCCGTCTCATAAGACGCCGCAACCACTGCCACCACCGGTTACTAAGCTGAAACCAAATGTCCCTCGCAACCCGCCACCCCAGAAGGGGGGCCATTCTTCCATGTTTAACGCATTTCAGGACACATGGACAGCCAGTTGCAAGGTGGCAGCATCAACCTTCATCGTCCCATCAAAATACGTCTTTTTAGTGGGCCCCATCTCCTTCTATGGTCCCTACTGCCAGCCCGATATCGTATTCCAGTTGATGGGACTTACGATTCAAGGAAATGGAATTATTGATGGAAGGGGTTCAGCATGGTGGCAAGTTTCACCTTATGATGATCCCATTGACGATGAATTCAAACTACTTATCCCACTCAACAACACTGTACAAGAAAAGCCACCCGTCCCGGTAAGAAGTGAGCTTGTGGGAAAGATGCCTAGGATCAAACCAACGGCTTTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAATAGTCCTCAATGCCACCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTACTGTTTCATCTCCAGGTGATAGTCTCAATACTGACGGAATTCATTTGCAAAATTCCAAGGATGTGCTAATCTACAGCTCTACTCTCTCTTGTGGTGATGACTGTATTTCAATACAAACGGGTTGCTCAAATGTGTATATACACAATGTCAATTGTGGACCAGGACATGGAATCAGCATTGGAAGTCTAGGGAAAGACAACACAAAGGCTTGTGTTTCTAACATCACTGTCCGTGATGTAATTATGCATAACACAATGAATGGTGTTAGAATTAAAACGTGGCAGGGTGGATTAGGTTTTGTACAAGGAGTCCTCTTCTCAAATATTCAGGTTTCTGAGGTTAAGATACCAATAGTTATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTACACAGTAAAGCCAGTACACTTTGCCTGTAGTGATAATCTGCCATGTACAGATATATCACTAACTGCAATTGAGCTCAAGCCACTGCAAGAACTCTATCATTTATACGAGGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGAGTGTTTGCAAATTGGGAAGCCATCAAGCAACCAAATTCAGTATGATTTTGACTCTTGTTGA

Coding sequence (CDS)

ATGAAAGGGATAAGTTTCAGAGGGCTCACATTATTTGTGTTTGTGTTGGCATTTCTTATCTGGTCTTCTAGTTTGGAGGGCTGCCATGGAAGAGCCACAAAGAAGCATTACTGGAGGAAGAACAGAGTGGCTGACGGTCACGGTGGCTCTCACAAAGGCAACCATCATGGTAGTGCATCAAAGACAAAGCCCTCGCCGTCTCATAAGACGCCGCAACCACTGCCACCACCGGTTACTAAGCTGAAACCAAATGTCCCTCGCAACCCGCCACCCCAGAAGGGGGGCCATTCTTCCATGTTTAACGCATTTCAGGACACATGGACAGCCAGTTGCAAGGTGGCAGCATCAACCTTCATCGTCCCATCAAAATACGTCTTTTTAGTGGGCCCCATCTCCTTCTATGGTCCCTACTGCCAGCCCGATATCGTATTCCAGTTGATGGGACTTACGATTCAAGGAAATGGAATTATTGATGGAAGGGGTTCAGCATGGTGGCAAGTTTCACCTTATGATGATCCCATTGACGATGAATTCAAACTACTTATCCCACTCAACAACACTGTACAAGAAAAGCCACCCGTCCCGGTAAGAAGTGAGCTTGTGGGAAAGATGCCTAGGATCAAACCAACGGCTTTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAATAGTCCTCAATGCCACCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTACTGTTTCATCTCCAGGTGATAGTCTCAATACTGACGGAATTCATTTGCAAAATTCCAAGGATGTGCTAATCTACAGCTCTACTCTCTCTTGTGGTGATGACTGTATTTCAATACAAACGGGTTGCTCAAATGTGTATATACACAATGTCAATTGTGGACCAGGACATGGAATCAGCATTGGAAGTCTAGGGAAAGACAACACAAAGGCTTGTGTTTCTAACATCACTGTCCGTGATGTAATTATGCATAACACAATGAATGGTGTTAGAATTAAAACGTGGCAGGGTGGATTAGGTTTTGTACAAGGAGTCCTCTTCTCAAATATTCAGGTTTCTGAGGTTAAGATACCAATAGTTATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTACACAGTAAAGCCAGTACACTTTGCCTGTAGTGATAATCTGCCATGTACAGATATATCACTAACTGCAATTGAGCTCAAGCCACTGCAAGAACTCTATCATTTATACGAGGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGAGTGTTTGCAAATTGGGAAGCCATCAAGCAACCAAATTCAGTATGATTTTGACTCTTGTTGA

Protein sequence

MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVADGHGGSHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFNAFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNTVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYEAFCWQTFGELRTPTVPPIECLQIGKPSSNQIQYDFDSC
Homology
[Close]
BLAST of Lsi04G005580 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.6e-85
Identity = 180/459 (39.22%), Postives = 241/459 (52.51%), Query Frame = 0 

Query: 51  HKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN--------- 110
           HKG  HG      P P        PP      P    +P P       +F+         
Sbjct: 32  HKG-RHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGD 91

Query: 111 -------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQLMGL----- 170
                  AFQD W A+C V +   + P   VF +    F GP C+P +VFQL G+     
Sbjct: 92  GSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLVFQLDGVLMPPD 151

Query: 171 -----------------------TIQGNGIIDGRGSAWWQV--SPYDDPIDDEFKLLIPL 230
                                  T  G G ++G G  WW +   P+  P           
Sbjct: 152 GPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSS------- 211

Query: 231 NNTVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL 290
                               P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C GVL
Sbjct: 212 -----------------SSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVL 271

Query: 291 VHDFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGH 350
           +++  +SSP  S NTDGIHL N++ V IY+S +S GDDCISI TGCS+V I  V CGP H
Sbjct: 272 INEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSH 331

Query: 351 GISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKI 410
           GISIGSLG  N++ACVSNITVR+ ++ ++ NG+R+KTWQGG G V  +LF NIQ+  V  
Sbjct: 332 GISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLN 391

Query: 411 PIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVK--PVHFACSDNLPCTDISLTAI 462
            I++DQ+YC    C N+TSAV +  + Y  I+GTY V+  P+HFACSD + CT+I+++ +
Sbjct: 392 CIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEV 451

[Close]
BLAST of Lsi04G005580 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.3e-62
Identity = 135/381 (35.43%), Postives = 194/381 (50.92%), Query Frame = 0 

Query: 102 AFQDTWTASCKVAAST-FIVPSKYVFLVGPISFYGPYCQPDIVFQLMG------------ 161
           AF D W  +C     T  +VP  Y  L+ PI   GP C+  +  Q+ G            
Sbjct: 71  AFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGP-CKARLTLQISGTIIAPNDPDVWE 130

Query: 162 ---------------LTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNTVQEKPP 221
                          LT++G G ++G G  WW+                    + +    
Sbjct: 131 GLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWE-------------------RSCKHNHS 190

Query: 222 VPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFTVSSP 281
            P R           PTAL F+   N+ V  + + +S Q H+   +C  V +    V +P
Sbjct: 191 NPCRG---------APTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAP 250

Query: 282 GDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGK 341
             S NTDGIH+  S+ ++I ++T+S GDDCISI    + + I N+ CGPGHGISIGSLGK
Sbjct: 251 ATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGK 310

Query: 342 DNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYC 401
             +   V +ITV   I+ +T NGVRIKTWQGG G V  ++F NI+++ V  PI+IDQ+YC
Sbjct: 311 SKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYC 370

Query: 402 DKAK-CSNQTSAVALSGINYERIRGTYTVK-PVHFACSDNLPCTDISLTAIELKPLQELY 453
           D  K C+NQTSA+++  I++  +RGT   K  +  +CSD+ PC +I L  I+L+P     
Sbjct: 371 DSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEP-SNGD 421

[Close]
BLAST of Lsi04G005580 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.2e-61
Identity = 131/358 (36.59%), Postives = 189/358 (52.79%), Query Frame = 0 

Query: 95  GHSSMFNAFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVF---------- 154
           G      AF   W A+CK  ++  +VP    F+V  + F GP CQP   F          
Sbjct: 70  GKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-CQPHFTFKVDGIIAAYQ 129

Query: 155 ---------------QLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNTVQ 214
                          +L G T+ G G+IDG+G  WW           + K          
Sbjct: 130 NPASWKNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWA---------GQCKW--------- 189

Query: 215 EKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFT 274
                 V    +    R +PTA++F  S  + + G+ + NSP+ HL F NC GV +   +
Sbjct: 190 ------VNGREICN-DRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGIS 249

Query: 275 VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIG 334
           +++P DS NTDGI +  SK+  +  +T+  GDDC++I TG SN+ I ++ CGPGHGISIG
Sbjct: 250 ITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIG 309

Query: 335 SLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVID 394
           SLG++N++A VS + V      +T NG+RIKTWQGG G    +++ N+++   + PI+I+
Sbjct: 310 SLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILIN 369

Query: 395 QFYCDKAK-CSNQTSAVALSGINYERIRGT-YTVKPVHFACSDNLPCTDISLTAIELK 426
           QFYC  A  C NQ SAV +  + Y+ IRGT  T   +   CSD++PC DI L+ I LK
Sbjct: 370 QFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLK 401

[Close]
BLAST of Lsi04G005580 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.1e-61
Identity = 136/359 (37.88%), Postives = 187/359 (52.09%), Query Frame = 0 

Query: 94  GGHSSMFNAFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVF--------- 153
           G H S   AF  TW A+CK A++  +VP+   F V  + F GP CQP + F         
Sbjct: 70  GKHDST-EAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGP-CQPHLSFKVDGTIVAQ 129

Query: 154 ----------------QLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNTV 213
                           QL    + G G+IDG+G  WW             +  +    TV
Sbjct: 130 PDPARWKNSKIWLQFAQLTDFNLMGTGVIDGQGQQWW-----------AGQCKVVNGRTV 189

Query: 214 QEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDF 273
                            R +PTA++   S +VTV  +T+ NSP+ HL F  C GV +   
Sbjct: 190 CN--------------DRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGL 249

Query: 274 TVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISI 333
            + +P DS NTDGI +  SK   I    +  GDDCI+I TG SN+ I ++ CGPGHGISI
Sbjct: 250 KIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISI 309

Query: 334 GSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVI 393
           GSLG+DN++A VS++ V      +T NG+RIKTWQGG G    + + N+++   + PI+I
Sbjct: 310 GSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILI 369

Query: 394 DQFYCDKAK-CSNQTSAVALSGINYERIRGT-YTVKPVHFACSDNLPCTDISLTAIELK 426
           +QFYC  A  C NQ SAV + G+ Y+ I GT  T   +   CSD++PCT I L+ + LK
Sbjct: 370 NQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLK 401

[Close]
BLAST of Lsi04G005580 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.2e-59
Identity = 129/361 (35.73%), Postives = 186/361 (51.52%), Query Frame = 0 

Query: 93  KGGHSSMFNAFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQLMG---- 152
           KG       AF+  W A+C   +S  ++  K  +LV PISF GP C+  +  Q+ G    
Sbjct: 98  KGDGRDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGP-CKSGLTMQIYGTIEA 157

Query: 153 ----------------------LTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNN 212
                                 L ++G G I+G G  WWQ                   N
Sbjct: 158 SDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQ-------------------N 217

Query: 213 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 272
           + +    +P +           PTAL FY S +V V  + I+N+ Q H+ FDNC+ V   
Sbjct: 218 SCKTNKALPCKD---------APTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQAS 277

Query: 273 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 332
           +  V++P +S NTDGIH+  ++++ I S  +  GDDCISI  G   V ++++ CGPGHGI
Sbjct: 278 NLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGI 337

Query: 333 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 392
           SIGSLG  N++A VS++ V    +  T NGVRIKTWQGG G    + F N+++  V+ PI
Sbjct: 338 SIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPI 397

Query: 393 VIDQFYCDKAK-CSNQTSAVALSGINYERIRGTYTVK-PVHFACSDNLPCTDISLTAIEL 426
           +IDQ YCD+ K C  Q+SAV +  + Y+ I+GT      + F CS   PC  I L  ++L
Sbjct: 398 IIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDL 429

[Close]
BLAST of Lsi04G005580 vs. ExPASy TrEMBL
Match: A0A1S3BFB4 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103489001 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 444/527 (84.25%), Postives = 456/527 (86.53%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+F +AFL+WSSSLEGCHGR   KHYWR+NRVA             HGG
Sbjct: 5   MEGISFRGLT-FMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQKGGHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQPDIVFQ        
Sbjct: 125 DGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNN 240
                             LMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+
Sbjct: 185 TDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNS 244

Query: 241 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           TVQEKPP P RSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 245 TVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 304

Query: 301 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 360
           DF VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI
Sbjct: 305 DFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 364

Query: 361 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 420
           SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI
Sbjct: 365 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 424

Query: 421 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 474
           VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP
Sbjct: 425 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 484

[Close]
BLAST of Lsi04G005580 vs. ExPASy TrEMBL
Match: A0A5D3CUP5 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001100 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 444/527 (84.25%), Postives = 456/527 (86.53%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+F +AFL+WSSSLEGCHGR   KHYWR+NRVA             HGG
Sbjct: 5   MEGISFRGLT-FMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQKGGHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQPDIVFQ        
Sbjct: 125 DGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNN 240
                             LMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+
Sbjct: 185 TDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNS 244

Query: 241 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           TVQEKPP P RSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 245 TVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 304

Query: 301 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 360
           DF VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI
Sbjct: 305 DFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 364

Query: 361 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 420
           SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI
Sbjct: 365 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 424

Query: 421 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 474
           VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP
Sbjct: 425 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 484

[Close]
BLAST of Lsi04G005580 vs. ExPASy TrEMBL
Match: E5GBI6 (Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 444/527 (84.25%), Postives = 456/527 (86.53%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+F +AFL+WSSSLEGCHGR   KHYWR+NRVA             HGG
Sbjct: 5   MEGISFRGLT-FMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQKGGHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQPDIVFQ        
Sbjct: 125 DGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNN 240
                             LMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+
Sbjct: 185 TDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNS 244

Query: 241 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           TVQEKPP P RSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 245 TVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 304

Query: 301 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 360
           DF VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI
Sbjct: 305 DFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 364

Query: 361 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 420
           SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI
Sbjct: 365 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 424

Query: 421 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 474
           VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP
Sbjct: 425 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 484

[Close]
BLAST of Lsi04G005580 vs. ExPASy TrEMBL
Match: A0A0A0KTN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601470 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.2e-254
Identity = 440/527 (83.49%), Postives = 453/527 (85.96%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+  +AFL+WSSSLEGCHG+   KHYWRKNRVA             HGG
Sbjct: 5   MEGISFRGLT-FMLAVAFLVWSSSLEGCHGKVRSKHYWRKNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQK GHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKEGHSIMFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQPDIVFQ        
Sbjct: 125 DGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNN 240
                             LMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+
Sbjct: 185 TDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNS 244

Query: 241 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           TVQEKPP P RSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 245 TVQEKPPPPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 304

Query: 301 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 360
           DF VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI
Sbjct: 305 DFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 364

Query: 361 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 420
           SIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI
Sbjct: 365 SIGSLGKDNTKACVSNITVRDVTMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 424

Query: 421 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 474
           VIDQFYCDKAKCSNQT+AVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP
Sbjct: 425 VIDQFYCDKAKCSNQTAAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 484

[Close]
BLAST of Lsi04G005580 vs. ExPASy TrEMBL
Match: A0A5A7SYF1 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003070 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.6e-253
Identity = 444/558 (79.57%), Postives = 456/558 (81.72%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+F +AFL+WSSSLEGCHGR   KHYWR+NRVA             HGG
Sbjct: 5   MEGISFRGLT-FMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQKGGHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAKG 124

Query: 121 ---------------------------------------AFQDTWTASCKVAASTFIVPS 180
                                                  AFQDTWTASCKVAASTFIVPS
Sbjct: 125 DGKTDDTKYVWWKWYFTGKGNGKGGVGPSRKAESRYGSWAFQDTWTASCKVAASTFIVPS 184

Query: 181 KYVFLVGPISFYGPYCQPDIVFQ--------------------------LMGLTIQGNGI 240
           KYVFLVGPISF GPYCQPDIVFQ                          LMGLTIQGNG+
Sbjct: 185 KYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGV 244

Query: 241 IDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNNTVQEKPPVPVRSELVGKMPRIKPTALRFY 300
           IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+TVQEKPP P RSELVGKMPRIKPTALRFY
Sbjct: 245 IDGRGSVWWQEISPYDDPIDDEFKLLIPLNSTVQEKPPAPARSELVGKMPRIKPTALRFY 304

Query: 301 GSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFTVSSPGDSLNTDGIHLQNSKDVLIYSS 360
           GSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDF VSSPGDSLNTDGIHLQNSKDVLIYSS
Sbjct: 305 GSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSS 364

Query: 361 TLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN 420
           TLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN
Sbjct: 365 TLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN 424

Query: 421 GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI 474
           GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI
Sbjct: 425 GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI 484

[Close]
BLAST of Lsi04G005580 vs. NCBI nr
Match: XP_008446197.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >XP_016900181.1 PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >ADN33828.1 polygalacturonase [Cucumis melo subsp. melo] >TYK15613.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 895.2 bits (2312), Expect = 2.4e-256
Identity = 444/527 (84.25%), Postives = 456/527 (86.53%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+F +AFL+WSSSLEGCHGR   KHYWR+NRVA             HGG
Sbjct: 5   MEGISFRGLT-FMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQKGGHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQPDIVFQ        
Sbjct: 125 DGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNN 240
                             LMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+
Sbjct: 185 TDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNS 244

Query: 241 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           TVQEKPP P RSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 245 TVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 304

Query: 301 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 360
           DF VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI
Sbjct: 305 DFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 364

Query: 361 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 420
           SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI
Sbjct: 365 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 424

Query: 421 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 474
           VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP
Sbjct: 425 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 484

[Close]
BLAST of Lsi04G005580 vs. NCBI nr
Match: XP_004135250.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN51797.1 hypothetical protein Csa_008759 [Cucumis sativus])

HSP 1 Score: 887.1 bits (2291), Expect = 6.6e-254
Identity = 440/527 (83.49%), Postives = 453/527 (85.96%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+  +AFL+WSSSLEGCHG+   KHYWRKNRVA             HGG
Sbjct: 5   MEGISFRGLT-FMLAVAFLVWSSSLEGCHGKVRSKHYWRKNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQK GHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKEGHSIMFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQPDIVFQ        
Sbjct: 125 DGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNN 240
                             LMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+
Sbjct: 185 TDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNS 244

Query: 241 TVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           TVQEKPP P RSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 245 TVQEKPPPPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 304

Query: 301 DFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 360
           DF VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI
Sbjct: 305 DFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGI 364

Query: 361 SIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 420
           SIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI
Sbjct: 365 SIGSLGKDNTKACVSNITVRDVTMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPI 424

Query: 421 VIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 474
           VIDQFYCDKAKCSNQT+AVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP
Sbjct: 425 VIDQFYCDKAKCSNQTAAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKP 484

[Close]
BLAST of Lsi04G005580 vs. NCBI nr
Match: XP_038893387.1 (polygalacturonase At1g48100-like isoform X2 [Benincasa hispida])

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-253
Identity = 438/526 (83.27%), Postives = 454/526 (86.31%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           MKGISFRGLT F+ ++AFL+WSSSLEGCHGRAT KHYWRKNRVA            GHGG
Sbjct: 5   MKGISFRGLT-FMCLVAFLVWSSSLEGCHGRATSKHYWRKNRVASPALSSKKGKAHGHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PS KTPQPLPPPV K KP++PR+PPPQKGGHSS+FN        
Sbjct: 65  SHKGNHHGSASKTKPPPSRKTPQPLPPPVAKPKPDIPRSPPPQKGGHSSIFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQP+IVFQ        
Sbjct: 125 DGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPNIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNT 240
                             L GLTIQGNG+IDGRGSAWWQVSPYDDPI+DEFK LIPLN+T
Sbjct: 185 TDFKVWGKGLLQWLQFTKLKGLTIQGNGVIDGRGSAWWQVSPYDDPIEDEFKQLIPLNST 244

Query: 241 VQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD 300
           ++EKPP P RSEL GKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD
Sbjct: 245 LKEKPPAPARSELEGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD 304

Query: 301 FTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS 360
           F VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS
Sbjct: 305 FFVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS 364

Query: 361 IGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIV 420
           IGSLGKDNTKACVSNITVRD+ MHNTM GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIV
Sbjct: 365 IGSLGKDNTKACVSNITVRDITMHNTMTGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIV 424

Query: 421 IDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPL 474
           IDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPC DISLTAIELKPL
Sbjct: 425 IDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCADISLTAIELKPL 484

[Close]
BLAST of Lsi04G005580 vs. NCBI nr
Match: KAA0034307.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 883.2 bits (2281), Expect = 9.6e-253
Identity = 444/558 (79.57%), Postives = 456/558 (81.72%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           M+GISFRGLT F+F +AFL+WSSSLEGCHGR   KHYWR+NRVA             HGG
Sbjct: 5   MEGISFRGLT-FMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PSHKTPQPLP PVTK KPN+PR+PPPQKGGHS MFN        
Sbjct: 65  SHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAKG 124

Query: 121 ---------------------------------------AFQDTWTASCKVAASTFIVPS 180
                                                  AFQDTWTASCKVAASTFIVPS
Sbjct: 125 DGKTDDTKYVWWKWYFTGKGNGKGGVGPSRKAESRYGSWAFQDTWTASCKVAASTFIVPS 184

Query: 181 KYVFLVGPISFYGPYCQPDIVFQ--------------------------LMGLTIQGNGI 240
           KYVFLVGPISF GPYCQPDIVFQ                          LMGLTIQGNG+
Sbjct: 185 KYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGV 244

Query: 241 IDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNNTVQEKPPVPVRSELVGKMPRIKPTALRFY 300
           IDGRGS WWQ +SPYDDPIDDEFKLLIPLN+TVQEKPP P RSELVGKMPRIKPTALRFY
Sbjct: 245 IDGRGSVWWQEISPYDDPIDDEFKLLIPLNSTVQEKPPAPARSELVGKMPRIKPTALRFY 304

Query: 301 GSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFTVSSPGDSLNTDGIHLQNSKDVLIYSS 360
           GSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDF VSSPGDSLNTDGIHLQNSKDVLIYSS
Sbjct: 305 GSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSS 364

Query: 361 TLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN 420
           TLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN
Sbjct: 365 TLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN 424

Query: 421 GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI 474
           GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI
Sbjct: 425 GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI 484

[Close]
BLAST of Lsi04G005580 vs. NCBI nr
Match: XP_038893386.1 (polygalacturonase At1g48100-like isoform X1 [Benincasa hispida])

HSP 1 Score: 880.2 bits (2273), Expect = 8.1e-252
Identity = 438/531 (82.49%), Postives = 454/531 (85.50%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVAD-----------GHGG 60
           MKGISFRGLT F+ ++AFL+WSSSLEGCHGRAT KHYWRKNRVA            GHGG
Sbjct: 5   MKGISFRGLT-FMCLVAFLVWSSSLEGCHGRATSKHYWRKNRVASPALSSKKGKAHGHGG 64

Query: 61  SHKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN-------- 120
           SHKGNHHGSASKTKP PS KTPQPLPPPV K KP++PR+PPPQKGGHSS+FN        
Sbjct: 65  SHKGNHHGSASKTKPPPSRKTPQPLPPPVAKPKPDIPRSPPPQKGGHSSIFNVLDFGAKG 124

Query: 121 --------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ-------- 180
                   AFQDTWTASCKVAASTFIVPSKYVFLVGPISF GPYCQP+IVFQ        
Sbjct: 125 DGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPNIVFQLDGTIIAP 184

Query: 181 ------------------LMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNT 240
                             L GLTIQGNG+IDGRGSAWWQVSPYDDPI+DEFK LIPLN+T
Sbjct: 185 TDFKVWGKGLLQWLQFTKLKGLTIQGNGVIDGRGSAWWQVSPYDDPIEDEFKQLIPLNST 244

Query: 241 VQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD 300
           ++EKPP P RSEL GKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD
Sbjct: 245 LKEKPPAPARSELEGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD 304

Query: 301 FTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS 360
           F VSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS
Sbjct: 305 FFVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS 364

Query: 361 IGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTW-----QGGLGFVQGVLFSNIQVSEV 420
           IGSLGKDNTKACVSNITVRD+ MHNTM GVRIKTW     QGGLGFVQGVLFSNIQVSEV
Sbjct: 365 IGSLGKDNTKACVSNITVRDITMHNTMTGVRIKTWQTVHLQGGLGFVQGVLFSNIQVSEV 424

Query: 421 KIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAI 474
           KIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPC DISLTAI
Sbjct: 425 KIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCADISLTAI 484

[Close]
BLAST of Lsi04G005580 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 624.0 bits (1608), Expect = 9.8e-179
Identity = 310/536 (57.84%), Postives = 379/536 (70.71%), Query Frame = 0 

Query: 2   KGISFRGLTLFVFVLAFLIWSSSLEGC---HGRATKKHYWRKNRVADGHGG----SHKGN 61
           + ++ R +T+   V   L+WS++LE C    GR  + H+   + ++D        SH+ N
Sbjct: 5   ESLTLRSITMMTLV-TILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENN 64

Query: 62  HHGSASKTKPSPSHKTPQPLP---PPVTKLKPNVPRNPPPQ------------------- 121
           HH  + K+KP P  KT  P      PV    P V + PPP                    
Sbjct: 65  HHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQF 124

Query: 122 -------KG-GHSSMFNAFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVF 181
                  KG G S    AF+  W ++CKV AST I+P  Y+FLVGPISF GPYCQ +IVF
Sbjct: 125 NVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYCQANIVF 184

Query: 182 Q--------------------------LMGLTIQGNGIIDGRGSAWWQVSPYDD-PIDDE 241
           Q                          L G+TIQGNG+IDGRG+ WWQ     D PIDD+
Sbjct: 185 QLEGMIVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDD 244

Query: 242 FKLLIPLNNTVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKF 301
           FKL++PLNN+VQE+PP+P+RSEL  +MP IKPTALRFYGS +VTVTGITIQNSPQCHLKF
Sbjct: 245 FKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKF 304

Query: 302 DNCIGVLVHDFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHN 361
           DNC+ VLVHD  VSSPGDS NTDGIHLQN++DV+I+++TL+CGDDCISIQTGCSNVY+HN
Sbjct: 305 DNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHN 364

Query: 362 VNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNI 421
           VNCGPGHGISIGSLGKD+TKACVSNITVRDV+MHNTM GVRIKTWQGG+G V+G+LFSNI
Sbjct: 365 VNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNI 424

Query: 422 QVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDI 474
           Q++EV++PIVIDQFYCD +KC N TSAV++ G+ YE+IRGTYTVKPVHFACSD+ PC D+
Sbjct: 425 QLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVKPVHFACSDSFPCIDV 484

[Close]
BLAST of Lsi04G005580 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 605.9 bits (1561), Expect = 2.8e-173
Identity = 307/536 (57.28%), Postives = 374/536 (69.78%), Query Frame = 0 

Query: 2   KGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWR-------------KNRVADGHG 61
           + +S R +T+ + ++A L+WS +LE C  R  +  +WR              N+    HG
Sbjct: 5   RSLSLRSITMMI-LMAVLVWSVTLETCIAR--RGRHWRHSHRSSSDLSDSLSNKKPKSHG 64

Query: 62  GSH----KGNHHGSASKTKPSPSHKTPQPL---PPPVTKLKPNVPRNP-PPQKGGHSSMF 121
            SH      N+H   SK KP P  KTP        PV    P V   P PPQKG  S +F
Sbjct: 65  NSHHHSSHNNNHHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPPQKG--SQVF 124

Query: 122 N----------------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVF 181
           N                AF+  W A+CK+ AS  +VP +Y +LVGPISF GPYCQ +IVF
Sbjct: 125 NVMDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVF 184

Query: 182 Q--------------------------LMGLTIQGNGIIDGRGSAWWQVSPYDDP-IDDE 241
           Q                          L G+ +QG G+IDGRGS WWQ    D P ID +
Sbjct: 185 QLDGTIIAPTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQ---QDSPFIDSD 244

Query: 242 FKLLIPLNNTVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKF 301
            KL++PLNN+  + PP+P+RSEL  +MP IKPTALRF GSF V VTGITIQNSPQCHLKF
Sbjct: 245 TKLIVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKF 304

Query: 302 DNCIGVLVHDFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHN 361
           D+C+GV+VHD  VSSPGDS NTDGIHLQN+KDVLI+S+TL+CGDDCISIQTGCSNV++HN
Sbjct: 305 DDCVGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHN 364

Query: 362 VNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNI 421
           VNCGPGHGISIGSLGK+ TKACVSNITVRDV MHNTM GVRIKTWQGG+G V+G++FSNI
Sbjct: 365 VNCGPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNI 424

Query: 422 QVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDI 474
           Q+++V+IPI I+QFYCD +KC NQTSAVA+ G+ YERI+GTYTVKPVHFACSDN PC D+
Sbjct: 425 QLNQVQIPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCVDV 484

[Close]
BLAST of Lsi04G005580 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 8.4e-130
Identity = 233/406 (57.39%), Postives = 272/406 (67.00%), Query Frame = 0 

Query: 95  GHSSMFNAFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQ--------- 154
           G S    AF+D W  +CKVAAST +VPS   FLVGP+SF G  C+  IVFQ         
Sbjct: 62  GTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKEKIVFQLEGKIIAPT 121

Query: 155 -----------------LMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNTV 214
                            L G+TI+G GIIDGRGS WW                       
Sbjct: 122 SASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWW----------------------- 181

Query: 215 QEKPPVPVRSELVG-KMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHD 274
                    ++++G KMPR KPTALRFYGS  VTV+GITIQNSPQ HLKFDNCI + V D
Sbjct: 182 ---------NDMMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSD 241

Query: 275 FTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGIS 334
           FT SSPGDS NTDGIHLQNS+D +IY STL+CGDDCISIQTGCSN+ IH+V+CGPGHGIS
Sbjct: 242 FTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGIS 301

Query: 335 IGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIV 394
           IG LGKDNTKACVSNITVRDV MH T NGVRIK+WQGG G V+ V+FSNIQVS V  PI+
Sbjct: 302 IGGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPII 361

Query: 395 IDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPL 454
           IDQ+YCD   C N+TSAVA+S INY  I+GTYT +PV FACSD+LPCT ISL+ IELKP 
Sbjct: 362 IDQYYCDGGGCHNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPA 421

Query: 455 QELYHLYEAFCWQTFGELRTPTVPPIECLQIGKPSSNQIQYDFDSC 474
                  + FCW+  GEL+T T+PPI+CL+  K      + + D+C
Sbjct: 422 TGKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

[Close]
BLAST of Lsi04G005580 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-96
Identity = 200/483 (41.41%), Postives = 274/483 (56.73%), Query Frame = 0 

Query: 1   MKGISFRGLTLFVFVLAFLIWSSSLEGCHGRATKKHYWRKNRVADGHGGSHKGNHHGSAS 60
           +K +    + L V ++A  I + S+EG      +KH+ +K +             H   S
Sbjct: 4   VKSLPILAMLLGVIIVAAAISTVSVEG------RKHHVKKIK-----------PKHRRHS 63

Query: 61  KTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFNAFQDTWTASCKVAASTFIV 120
           K  P+ S   P P P P T        +   +  G S    A    W A+CKV      +
Sbjct: 64  KNTPTGS---PAPAPYPSTNEGVFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEI 123

Query: 121 PSKYVFLVGPISFYGPYCQPDIVFQLMG---------------------------LTIQG 180
           P+   FLV  ++  GP C+ + V Q+ G                           LTIQG
Sbjct: 124 PAGTQFLVKAVTLQGP-CKEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQG 183

Query: 181 NGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNNTVQEKPPVPVRSELVGKMPRIKPTALR 240
           +G ++GRG  WW +  Y     +++                         +P +KPTALR
Sbjct: 184 SGTLNGRGYNWWNLDTYQTQTRNKY-------------------------IPPMKPTALR 243

Query: 241 FYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFTVSSPGDSLNTDGIHLQNSKDVLIY 300
           FY S NVTV  I+I NSP CHLKFD+  GV V++ T+SSP +S NTDGIHLQN+++V I 
Sbjct: 244 FYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPENSPNTDGIHLQNTRNVEIQ 303

Query: 301 SSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNT 360
            S ++CGDDC+SIQTG SNV+IH++NCGPGHGISIG LGKD + ACVS+I V D+ + NT
Sbjct: 304 HSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKSVACVSDIIVEDISIQNT 363

Query: 361 MNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYE 420
           + GVRIKTWQGGLG V+ + FSNIQV +VK+PIVIDQ+YCDK+KC NQT AV++SG+ Y 
Sbjct: 364 LAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDKSKCKNQTRAVSISGVKYN 423

Query: 421 RIRGTYTVKPVHFACSDNLPCTDISLTAIELKPL-----QELYHLYEAFCWQTFGELRTP 452
            I G++TV+PV  ACS+N+PC D+ L  I L+P       + +   +A CW ++G+ + P
Sbjct: 424 NIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQTHQQQQALCWNSYGKTQGP 440

[Close]
BLAST of Lsi04G005580 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 1.1e-86
Identity = 180/459 (39.22%), Postives = 241/459 (52.51%), Query Frame = 0 

Query: 51  HKGNHHGSASKTKPSPSHKTPQPLPPPVTKLKPNVPRNPPPQKGGHSSMFN--------- 110
           HKG  HG      P P        PP      P    +P P       +F+         
Sbjct: 32  HKG-RHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGD 91

Query: 111 -------AFQDTWTASCKVAASTFIVPSKYVFLVGPISFYGPYCQPDIVFQLMGL----- 170
                  AFQD W A+C V +   + P   VF +    F GP C+P +VFQL G+     
Sbjct: 92  GSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLVFQLDGVLMPPD 151

Query: 171 -----------------------TIQGNGIIDGRGSAWWQV--SPYDDPIDDEFKLLIPL 230
                                  T  G G ++G G  WW +   P+  P           
Sbjct: 152 GPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSS------- 211

Query: 231 NNTVQEKPPVPVRSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL 290
                               P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C GVL
Sbjct: 212 -----------------SSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVL 271

Query: 291 VHDFTVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGH 350
           +++  +SSP  S NTDGIHL N++ V IY+S +S GDDCISI TGCS+V I  V CGP H
Sbjct: 272 INEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSH 331

Query: 351 GISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKI 410
           GISIGSLG  N++ACVSNITVR+ ++ ++ NG+R+KTWQGG G V  +LF NIQ+  V  
Sbjct: 332 GISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLN 391

Query: 411 PIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVK--PVHFACSDNLPCTDISLTAI 462
            I++DQ+YC    C N+TSAV +  + Y  I+GTY V+  P+HFACSD + CT+I+++ +
Sbjct: 392 CIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEV 451

The following BLAST results are available for this feature:
BLAST of Lsi04G005580 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q949Z11.6e-8539.22Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ57.3e-6235.43Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432121.2e-6136.59Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E72.1e-6137.88Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P353361.2e-5935.73Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
BLAST of Lsi04G005580 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BFB41.2e-25684.25polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103489001 PE=3 SV... [more]
A0A5D3CUP51.2e-25684.25Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
E5GBI61.2e-25684.25Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A0A0KTN93.2e-25483.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601470 PE=3 SV=1[more]
A0A5A7SYF14.6e-25379.57Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003... [more]
BLAST of Lsi04G005580 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008446197.12.4e-25684.25PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >XP_016900181.1 PREDI... [more]
XP_004135250.16.6e-25483.49polygalacturonase At1g48100 [Cucumis sativus] >KGN51797.1 hypothetical protein C... [more]
XP_038893387.11.1e-25383.27polygalacturonase At1g48100-like isoform X2 [Benincasa hispida][more]
KAA0034307.19.6e-25379.57polygalacturonase [Cucumis melo var. makuwa][more]
XP_038893386.18.1e-25282.49polygalacturonase At1g48100-like isoform X1 [Benincasa hispida][more]
BLAST of Lsi04G005580 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G60590.19.8e-17957.84Pectin lyase-like superfamily protein [more]
AT1G10640.12.8e-17357.28Pectin lyase-like superfamily protein [more]
AT5G14650.18.4e-13057.39Pectin lyase-like superfamily protein [more]
AT3G26610.11.6e-9641.41Pectin lyase-like superfamily protein [more]
AT1G48100.11.1e-8639.22Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 240..266
e-value: 130.0
score: 11.4
coord: 320..341
e-value: 4.3
score: 16.3
coord: 267..288
e-value: 560.0
score: 9.0
coord: 290..310
e-value: 4000.0
score: 2.9
coord: 349..370
e-value: 7400.0
score: 1.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 94..463
e-value: 1.4E-96
score: 325.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 206..425
e-value: 4.0E-68
score: 229.9
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 297..310
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..90
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 9..49
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 95..148
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 95..148
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 9..49
coord: 146..463
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 146..463
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 95..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G005580.1Lsi04G005580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity