Lsi04G005290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G005290
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING-type domain-containing protein
Locationchr04: 4879568 .. 4880674 (+)
RNA-Seq ExpressionLsi04G005290
SyntenyLsi04G005290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATTTTGTTCATCCTCAAACCAGAGATTTTCTGGTTAGTCCCTCAATTTCCCCTTTGCATTCTTCTTCTTCTTCTTCTTCATTAGCATATAACAGTGACTACCAAAAACAAGAACCAACAACTCCACTCACTTCCTCTAGCAGTAAAATAAGCCCTGCAGTTCTTTTAGTCATAGTTATTCTGGCACTTTTCCTTTTCATCTCTGGTCTTCTTCACTTGCTAGTGAGATTACTTGTAAAGCAACGTTCCTCATCATCATCAATATCTGAATCCAATAGCAATAGATTCCCAGAAATGTCTGAATCATCTGCTTTTCAAAGACAGTTACAACAGCTCTTCCATCTTCATGATTCAGGTCTAGATCAAGCCTTCATAGATGCCCTCCCTGTTTTCCTTTACAAAGAGATAGTGGGTATAAAAGAACCATTTGATTGTGCTGTATGTCTTTGTGAATTTTCAGAACTAGACAAGCTAAGATTGCTCCCTACTTGCAGTCATGCTTTTCACATTGATTGTATAGACACATGGTTGCTTTCTAATTCAACTTGCCCTCTTTGTAGAGGGACCCTTCACATTCACTCACCTGGACTTGCCATAGAAAACCCTGTATATGGTTTTGAAGATTCAGAAGAAACTGAAGAGTCTACAGAGGATGGAAGATTTGGTATTCTTACAGCTCAAAAAGCTCCAGAGAGTGAAATTATTGGTGAAAAGAGAATGTTTTCAATCAGACTTGGCAAATTTAGGAACTTGAATAATGGAGGATTGAGAGGGTTAGAGAAAGGAGAGGGAGAAACCAGCAGTAGCAGTTTGAGTGCAAGAAGATGCTATTCAATGGGGTCCTATCAGTATATTGTGGCTGATTCAGAATTGCAAGTCGCGTTGCGTACGAGTAATCGTATTGGTGGGGGAAGTATGAGATTTGTGAAAGTAATAAGAGATTCACAAACTGGGAATTGCTCAGTTGTTGATGATGATGATGCTGAGGGGAAGAAGATTAACATTGGAAGTAAAAATGAAAGCTTTTCTGTTTCAAAAATCTGGTTATGGTCCAAGAAGTCTCGACAGCCAAGTGGTTCAGAAACCCATGTGCGAGCTAGTTAA

mRNA sequence

ATGTATTTTGTTCATCCTCAAACCAGAGATTTTCTGGTTAGTCCCTCAATTTCCCCTTTGCATTCTTCTTCTTCTTCTTCTTCATTAGCATATAACAGTGACTACCAAAAACAAGAACCAACAACTCCACTCACTTCCTCTAGCAGTAAAATAAGCCCTGCAGTTCTTTTAGTCATAGTTATTCTGGCACTTTTCCTTTTCATCTCTGGTCTTCTTCACTTGCTAGTGAGATTACTTGTAAAGCAACGTTCCTCATCATCATCAATATCTGAATCCAATAGCAATAGATTCCCAGAAATGTCTGAATCATCTGCTTTTCAAAGACAGTTACAACAGCTCTTCCATCTTCATGATTCAGGTCTAGATCAAGCCTTCATAGATGCCCTCCCTGTTTTCCTTTACAAAGAGATAGTGGGTATAAAAGAACCATTTGATTGTGCTGTATGTCTTTGTGAATTTTCAGAACTAGACAAGCTAAGATTGCTCCCTACTTGCAGTCATGCTTTTCACATTGATTGTATAGACACATGGTTGCTTTCTAATTCAACTTGCCCTCTTTGTAGAGGGACCCTTCACATTCACTCACCTGGACTTGCCATAGAAAACCCTGTATATGGTTTTGAAGATTCAGAAGAAACTGAAGAGTCTACAGAGGATGGAAGATTTGGTATTCTTACAGCTCAAAAAGCTCCAGAGAGTGAAATTATTGGTGAAAAGAGAATGTTTTCAATCAGACTTGGCAAATTTAGGAACTTGAATAATGGAGGATTGAGAGGGTTAGAGAAAGGAGAGGGAGAAACCAGCAGTAGCAGTTTGAGTGCAAGAAGATGCTATTCAATGGGGTCCTATCAGTATATTGTGGCTGATTCAGAATTGCAAGTCGCGTTGCGTACGAGTAATCGTATTGGTGGGGGAAGTATGAGATTTGTGAAAGTAATAAGAGATTCACAAACTGGGAATTGCTCAGTTGTTGATGATGATGATGCTGAGGGGAAGAAGATTAACATTGGAAGTAAAAATGAAAGCTTTTCTGTTTCAAAAATCTGGTTATGGTCCAAGAAGTCTCGACAGCCAAGTGGTTCAGAAACCCATGTGCGAGCTAGTTAA

Coding sequence (CDS)

ATGTATTTTGTTCATCCTCAAACCAGAGATTTTCTGGTTAGTCCCTCAATTTCCCCTTTGCATTCTTCTTCTTCTTCTTCTTCATTAGCATATAACAGTGACTACCAAAAACAAGAACCAACAACTCCACTCACTTCCTCTAGCAGTAAAATAAGCCCTGCAGTTCTTTTAGTCATAGTTATTCTGGCACTTTTCCTTTTCATCTCTGGTCTTCTTCACTTGCTAGTGAGATTACTTGTAAAGCAACGTTCCTCATCATCATCAATATCTGAATCCAATAGCAATAGATTCCCAGAAATGTCTGAATCATCTGCTTTTCAAAGACAGTTACAACAGCTCTTCCATCTTCATGATTCAGGTCTAGATCAAGCCTTCATAGATGCCCTCCCTGTTTTCCTTTACAAAGAGATAGTGGGTATAAAAGAACCATTTGATTGTGCTGTATGTCTTTGTGAATTTTCAGAACTAGACAAGCTAAGATTGCTCCCTACTTGCAGTCATGCTTTTCACATTGATTGTATAGACACATGGTTGCTTTCTAATTCAACTTGCCCTCTTTGTAGAGGGACCCTTCACATTCACTCACCTGGACTTGCCATAGAAAACCCTGTATATGGTTTTGAAGATTCAGAAGAAACTGAAGAGTCTACAGAGGATGGAAGATTTGGTATTCTTACAGCTCAAAAAGCTCCAGAGAGTGAAATTATTGGTGAAAAGAGAATGTTTTCAATCAGACTTGGCAAATTTAGGAACTTGAATAATGGAGGATTGAGAGGGTTAGAGAAAGGAGAGGGAGAAACCAGCAGTAGCAGTTTGAGTGCAAGAAGATGCTATTCAATGGGGTCCTATCAGTATATTGTGGCTGATTCAGAATTGCAAGTCGCGTTGCGTACGAGTAATCGTATTGGTGGGGGAAGTATGAGATTTGTGAAAGTAATAAGAGATTCACAAACTGGGAATTGCTCAGTTGTTGATGATGATGATGCTGAGGGGAAGAAGATTAACATTGGAAGTAAAAATGAAAGCTTTTCTGTTTCAAAAATCTGGTTATGGTCCAAGAAGTCTCGACAGCCAAGTGGTTCAGAAACCCATGTGCGAGCTAGTTAA

Protein sequence

MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKRMFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGSETHVRAS
Homology
BLAST of Lsi04G005290 vs. ExPASy Swiss-Prot
Match: Q9FL07 (RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-86
Identity = 199/344 (57.85%), Postives = 243/344 (70.64%), Query Frame = 0

Query: 40  PTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPE 99
           P+ P  SS ++ISPAVL VIVILA+  FISGLLHLLVR L+K  S+++S   S SNRFPE
Sbjct: 30  PSPPPPSSGNRISPAVLFVIVILAVLFFISGLLHLLVRFLIKHPSATAS---SRSNRFPE 89

Query: 100 MSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVG----------IKEPFDCAVC 159
           +S S A QRQLQQLFHL+DSGLDQAFIDALPVF YKEIVG           +EPFDCAVC
Sbjct: 90  ISTSDALQRQLQQLFHLNDSGLDQAFIDALPVFHYKEIVGSAGGGGGNGAAQEPFDCAVC 149

Query: 160 LCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIHSPGLAIENPVYGFED 219
           LCEFSE DKLRLLP CSHAFH++CIDTWL SNSTCPLCRGTL   SPG ++ENP++ F+D
Sbjct: 150 LCEFSEKDKLRLLPMCSHAFHLNCIDTWLQSNSTCPLCRGTL--FSPGFSMENPMFDFDD 209

Query: 220 SEETEES-TEDGRFGILTAQKAPE-SEIIGEKRMFSIRLGKFRNLNN-GGLRGLE-KGEG 279
             E EE  TE+G      +QK  E  EI+ EK +  +RLGKF+ L+N G  +G +    G
Sbjct: 210 IREDEEGVTENG------SQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGG 269

Query: 280 ETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIRDS-QTGNCSVV 339
           ETSSS+L ARRC+SMGSYQYI+ +SEL+V    ++R+     R     ++S QTGN S  
Sbjct: 270 ETSSSNLDARRCFSMGSYQYILGNSELKVPF-ANDRL----PRLKPQDKESEQTGNSSSE 329

Query: 340 DDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGSETHVRAS 369
           D+     KKIN  +K ESFSVSKIWLW KK +  S ++  + +S
Sbjct: 330 DN-----KKINTVAKGESFSVSKIWLWPKKDKFSSDAQRRLPSS 352

BLAST of Lsi04G005290 vs. ExPASy Swiss-Prot
Match: Q8GW38 (RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.9e-82
Identity = 190/362 (52.49%), Postives = 242/362 (66.85%), Query Frame = 0

Query: 12  LVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGL 71
           L + +++ +  SS+ S +  N        ++  +  +++ISP +L +IV+L++  FI  +
Sbjct: 11  LKNDNLNQISPSSAPSPITLNHQLTDSSSSSS-SGGNNRISPIILFIIVLLSVIFFICSI 70

Query: 72  LHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPV 131
           LHLLVR  +K++ S+ S S + SN+ PE S+S  +QRQLQQLFHLHDSGLDQA IDALPV
Sbjct: 71  LHLLVRYYLKKKRSNLSSSPNESNQNPEFSDSDTYQRQLQQLFHLHDSGLDQALIDALPV 130

Query: 132 FLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTL 191
           FLYKEI G KEPFDCAVCLCEFSE DKLRLLP CSHAFHIDCIDTWLLSNSTCPLCRGTL
Sbjct: 131 FLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHAFHIDCIDTWLLSNSTCPLCRGTL 190

Query: 192 HIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTA--QKAPESEIIGEKRMFSIRLGKF 251
              S G   E P + F        + +DG  G+  +  QK  E+EI   KR+FS+RLGKF
Sbjct: 191 --FSLGHQFEYPDFNFGFF-----AGDDGGGGVRVSPVQKPAENEI--GKRVFSVRLGKF 250

Query: 252 RN---LNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGS 311
           R+   +NNG +  +  G GETSSSSL  RRC+SMGSYQYIVA+S+L VAL  +N      
Sbjct: 251 RSSNIVNNGEV--VVGGGGETSSSSLDNRRCFSMGSYQYIVAESDLVVALCPNNE----- 310

Query: 312 MRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWS-KKSRQPSG--SET 366
                           + ++ D EGKKIN+ SK ESFSVSKIW WS K+S+ P+   SET
Sbjct: 311 ---------------GLKNNKDVEGKKINMRSKGESFSVSKIWQWSNKRSKFPNNHPSET 340

BLAST of Lsi04G005290 vs. ExPASy Swiss-Prot
Match: Q940Q4 (RING-H2 finger protein ATL13 OS=Arabidopsis thaliana OX=3702 GN=ATL13 PE=2 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.5e-54
Identity = 160/399 (40.10%), Postives = 210/399 (52.63%), Query Frame = 0

Query: 13  VSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSS-----SSKISPAVLLVIVILALFLF 72
           V P I    +  S SSL        QEP   L SS     +SKISP++LL+I+IL++  F
Sbjct: 11  VFPEIKTTQNFLSPSSL-------PQEPPLSLRSSANFDLNSKISPSILLIIIILSIIFF 70

Query: 73  ISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFID 132
           ISGLLHLLVR L         ++ S+ +R       +A Q QLQQLFHLHDSG+DQ+FID
Sbjct: 71  ISGLLHLLVRFL---------LTPSSRDREDYFDNVTALQGQLQQLFHLHDSGVDQSFID 130

Query: 133 ALPVFLYKEIVGIKE-PFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPL 192
            LPVF YK I+G+K  PFDCAVCLCEF   DKLRLLP CSHAFH+DCIDTWLLS+STCPL
Sbjct: 131 TLPVFHYKSIIGLKNYPFDCAVCLCEFETEDKLRLLPKCSHAFHMDCIDTWLLSHSTCPL 190

Query: 193 CRGT----------------LHIHSPGLAIENPVYGFEDSEETEESTED-----GRFGIL 252
           CR +                L + S        + G  DS     + +D        G++
Sbjct: 191 CRSSLLSDLSSHQDPRSSFLLVLESASDHSSREIGGDRDSAACVAANDDIDVSSAHLGLV 250

Query: 253 TAQKAPESEI----------------IGEKRMFSIRLGKFRNLNNGGLRGLEKGEGETSS 312
                  + I                +G+   FS++LGKFRN++ G   G        +S
Sbjct: 251 GNNDLGSTRIDSGHGDQYLDGELGGSVGKVVPFSVKLGKFRNIDIG--EGTSSNNNIGNS 310

Query: 313 SSLSARRCYSMGSYQYIV-ADSELQVALRT-----------SNRIGGGSMRFVKVIRDSQ 357
           SSL  RRC+SMGSY+YI+  ++ L+V + T            +R       F    R   
Sbjct: 311 SSLDERRCFSMGSYEYIMDEETTLKVHVSTKKQSSKNRGLPGHRTAMSECGFDPTGRLKF 370

BLAST of Lsi04G005290 vs. ExPASy Swiss-Prot
Match: Q9ZV53 (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.7e-51
Identity = 139/347 (40.06%), Postives = 199/347 (57.35%), Query Frame = 0

Query: 42  TPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMS 101
           T L++ +SKI+P +LL+I+IL++  FISGLLH+LV+ L         ++ S  +R     
Sbjct: 30  TSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFL---------LTPSRESREDYFD 89

Query: 102 ESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGIK-EPFDCAVCLCEFSELDKLR 161
             +A Q QLQQLF+LHDSG+DQ+ ID LPVF YK IVG+K  PFDC VCLCEF   DKLR
Sbjct: 90  NVTALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFETEDKLR 149

Query: 162 LLPTCSHAFHIDCIDTWLLSNSTCPLCRGTL--------HIHSPGLAI------------ 221
           LLP CSHAFH++CIDTWLLS+STCPLCR  L        ++ S  L +            
Sbjct: 150 LLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQSSRDMVP 209

Query: 222 ---ENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKRM-FSIRLGKFRNLNNGG 281
               N   G++ + ++E +    R G  +     + + + EK +   ++LGKFRN+++ G
Sbjct: 210 VLESNSQLGYDVNNDSESTRI--RSGRKSCDPDGDMDGLDEKVVPLEVKLGKFRNIDHVG 269

Query: 282 LRGLEKGEGETS--SSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIR 341
             G ++ +   S  S ++  RRC SMGSY+YI+ D E  + +  S +   G  R    + 
Sbjct: 270 -EGSDQKKNSISGNSKNVDGRRCLSMGSYEYIM-DQEATLKVHVSTKKLSGKDR----VP 329

Query: 342 DSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGS 362
             +T       D   +G + ++  + ESFS+SKIWL  KK +Q   S
Sbjct: 330 SHRTVMSECGFDPTVKGIEKSV-VERESFSLSKIWLRGKKEKQKGTS 358

BLAST of Lsi04G005290 vs. ExPASy Swiss-Prot
Match: Q7X843 (RING-H2 finger protein ATL48 OS=Arabidopsis thaliana OX=3702 GN=ATL48 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.9e-41
Identity = 121/289 (41.87%), Postives = 165/289 (57.09%), Query Frame = 0

Query: 16  SISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLL 75
           ++SP  SS+++ +L    D            SSS I   + LVI  LAL +   G+L+L+
Sbjct: 92  ALSPKSSSAATLTLMNQKD-----------PSSSSIVSVLCLVISGLALIIVFLGVLYLI 151

Query: 76  VRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYK 135
            + L   R SS+     + N  P++   S+   QLQ LF LHDSGLDQ  IDALPVFLY 
Sbjct: 152 FKFL---RKSSTLFPIPHFNYNPDLFSFSS--PQLQHLFFLHDSGLDQTAIDALPVFLYG 211

Query: 136 EI-VGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIH 195
            + + +++PFDCAVCL EFS+ DKLRLLP CSHAFH+ CIDTWLLSNSTCPLCR +    
Sbjct: 212 NVTISLEQPFDCAVCLNEFSDTDKLRLLPVCSHAFHLHCIDTWLLSNSTCPLCRRS---- 271

Query: 196 SPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKRMFSIRLGKFRNLNN 255
              L+  N  Y   +  ET  +   G       Q+  + +    KR+FS+RLG+F++ N 
Sbjct: 272 ---LSTSNVCY---NHSETLVAPLSGH------QQVDDGKASLAKRVFSVRLGRFKSTNE 331

Query: 256 GGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIG 304
                 ++ + +        RRCYSMG+ QY+V D +  VAL +S R G
Sbjct: 332 S---QSQRHDVKDEIGVRMPRRCYSMGTQQYLVCDQDFVVALSSSPREG 345

BLAST of Lsi04G005290 vs. ExPASy TrEMBL
Match: A0A1S3BE30 (RING-H2 finger protein ATL46 OS=Cucumis melo OX=3656 GN=LOC103489027 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.0e-175
Identity = 331/363 (91.18%), Postives = 342/363 (94.21%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M  +HPQTRDFLVSPSISPLH    SSSLAY+ DYQK EPT P TSSSSKISPAVLLVIV
Sbjct: 1   MSLIHPQTRDFLVSPSISPLH----SSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIV 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSE-SSAFQRQLQQLFHLHDS 120
           ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPE+SE SSAFQRQLQQLFHLHDS
Sbjct: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPELSESSSAFQRQLQQLFHLHDS 120

Query: 121 GLDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL 180
           GLDQAFIDALPVFLYKEIVG+KEPFDCAVCLCEFSELDKLRLLP+CSHAFHIDCIDTWLL
Sbjct: 121 GLDQAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPSCSHAFHIDCIDTWLL 180

Query: 181 SNSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEK 240
           SNSTCPLCRGTLHI SP LAIENPVYGFEDSEETEESTE+GRFGILTAQKAPES+IIGEK
Sbjct: 181 SNSTCPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEK 240

Query: 241 RMFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTS 300
           R+FSIRLGKFRNLNNGGLRGLEKGEGETSSSSL+ARRCYSMGSYQYIVA+SELQVAL TS
Sbjct: 241 RVFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLNARRCYSMGSYQYIVAESELQVALHTS 300

Query: 301 NRIGGGSMRFVKVIRDSQTGNCSV-VDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQP 360
           NRI GGSMRFVKVIRDSQTGNC +  DDDDAE KKINIGSKNESFSVSKIWLWSKKSRQP
Sbjct: 301 NRISGGSMRFVKVIRDSQTGNCPIDHDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQP 359

Query: 361 SGS 362
             S
Sbjct: 361 CSS 359

BLAST of Lsi04G005290 vs. ExPASy TrEMBL
Match: A0A0A0KVN9 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G602200 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.6e-174
Identity = 331/363 (91.18%), Postives = 340/363 (93.66%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M   HPQTRDF VSPSISPLH    SSSLAY+ DYQK EPT P TSSSSKISPAVLLVIV
Sbjct: 1   MSLSHPQTRDFPVSPSISPLH----SSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIV 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSE-SSAFQRQLQQLFHLHDS 120
           ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFP++SE SSAFQRQLQQLFHLHDS
Sbjct: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPDLSESSSAFQRQLQQLFHLHDS 120

Query: 121 GLDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL 180
           GLDQAFIDALPVFLYKEIVG+KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL
Sbjct: 121 GLDQAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL 180

Query: 181 SNSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEK 240
           SNSTCPLCRGTLHI SP LAIENPVYGFEDSEETEESTE+GRFGILTAQKAPES+IIGEK
Sbjct: 181 SNSTCPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEK 240

Query: 241 RMFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTS 300
           R+FSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVA+SELQVAL TS
Sbjct: 241 RVFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVAESELQVALHTS 300

Query: 301 NRIGGGSMRFVKVIRDSQTGNCSV-VDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQP 360
           NRI GGSMRFVKVIRDSQTGNC +  DDDDAE KKINIGSKNESFSVSKIWLWSKKSRQP
Sbjct: 301 NRISGGSMRFVKVIRDSQTGNCPIDRDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQP 359

Query: 361 SGS 362
             S
Sbjct: 361 CSS 359

BLAST of Lsi04G005290 vs. ExPASy TrEMBL
Match: A0A5D3CZH7 (RING-H2 finger protein ATL46 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00840 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.8e-164
Identity = 309/335 (92.24%), Postives = 320/335 (95.52%), Query Frame = 0

Query: 29  LAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSS 88
           +AY+ DYQK EPT P TSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSS
Sbjct: 21  VAYDIDYQKLEPTPPSTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSS 80

Query: 89  ISESNSNRFPEMSE-SSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGIKEPFDCA 148
           ISESNSNRFPE+SE SSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVG+KEPFDCA
Sbjct: 81  ISESNSNRFPELSESSSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGLKEPFDCA 140

Query: 149 VCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIHSPGLAIENPVYGF 208
           VCLCEFSELDKLRLLP+CSHAFHIDCIDTWLLSNSTCPLCRGTLHI SP LAIENPVYGF
Sbjct: 141 VCLCEFSELDKLRLLPSCSHAFHIDCIDTWLLSNSTCPLCRGTLHIQSPVLAIENPVYGF 200

Query: 209 EDSEETEESTEDGRFGILTAQKAPESEIIGEKRMFSIRLGKFRNLNNGGLRGLEKGEGET 268
           EDSEETEESTE+GRFGILTAQKAPES+IIGEKR+FSIRLGKFRNLNNGGLRGLEKGEGET
Sbjct: 201 EDSEETEESTENGRFGILTAQKAPESDIIGEKRVFSIRLGKFRNLNNGGLRGLEKGEGET 260

Query: 269 SSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIRDSQTGNCSV-VDD 328
           SSSSL+ARRCYSMGSYQYIVA+SELQVAL TSNRI GGSMRFVKVIRDSQTGNC +  DD
Sbjct: 261 SSSSLNARRCYSMGSYQYIVAESELQVALHTSNRISGGSMRFVKVIRDSQTGNCPIDHDD 320

Query: 329 DDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGS 362
           DDAE KKINIGSKNESFSVSKIWLWSKKSRQP  S
Sbjct: 321 DDAERKKINIGSKNESFSVSKIWLWSKKSRQPCSS 355

BLAST of Lsi04G005290 vs. ExPASy TrEMBL
Match: A0A6J1DCR2 (RING-H2 finger protein ATL47 OS=Momordica charantia OX=3673 GN=LOC111019155 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.7e-157
Identity = 304/368 (82.61%), Postives = 326/368 (88.59%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M F+HPQTR FLV+PSISPLHSSS  +       YQKQ   TP TSSSSKISPAVLLVIV
Sbjct: 1   MSFLHPQTRVFLVTPSISPLHSSSPLA-------YQKQ--PTPPTSSSSKISPAVLLVIV 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG 120
           ILA+  FISG+LH LVR LVKQR S SSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG
Sbjct: 61  ILAILFFISGILHFLVRFLVKQR-SPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG 120

Query: 121 LDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS 180
           LDQAFIDALPVFLY EIVG+KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS
Sbjct: 121 LDQAFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS 180

Query: 181 NSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKR 240
           NSTCPLCRGTL  +SPGLAIENP++GFEDSE TE S EDGR G+ TAQK PE+++ GEKR
Sbjct: 181 NSTCPLCRGTL--YSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKR 240

Query: 241 MFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSN 300
           +FSIRLGKFRNLNNGG+RGLEKGEGETSSSSL ARRCYSMGSYQYIVADS+LQVALRTSN
Sbjct: 241 VFSIRLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSN 300

Query: 301 RIGGGSMRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSG 360
           R GG SMRFV+  RDSQTGN  +  DDDAEGKKINIGSKNESFSVSKIWLWSKK+RQP G
Sbjct: 301 RTGGRSMRFVRA-RDSQTGNSLI--DDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIG 353

Query: 361 SETHVRAS 369
           S+ H+RA+
Sbjct: 361 SDVHLRAN 353

BLAST of Lsi04G005290 vs. ExPASy TrEMBL
Match: A0A6J1JJB0 (RING-H2 finger protein ATL46-like OS=Cucurbita maxima OX=3661 GN=LOC111486314 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.3e-154
Identity = 309/367 (84.20%), Postives = 323/367 (88.01%), Query Frame = 0

Query: 2   YFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVI 61
           + VHPQTRD LVSPSI+PL     SSSLAYNSDY KQ  + P +SSSSKISPAVLLVIVI
Sbjct: 3   FVVHPQTRDILVSPSIAPL---QPSSSLAYNSDYHKQ--SAPPSSSSSKISPAVLLVIVI 62

Query: 62  LALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGL 121
           LAL  FISGLLHLLVRLLVKQRSS SSI ESNSNRF EMS+SSAFQRQLQQLFHLHDSGL
Sbjct: 63  LALLFFISGLLHLLVRLLVKQRSSPSSIPESNSNRFLEMSDSSAFQRQLQQLFHLHDSGL 122

Query: 122 DQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSN 181
           DQAFIDALPVFLYKEIVG+KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSN
Sbjct: 123 DQAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSN 182

Query: 182 STCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDG-RFGILTAQKAPESEIIGEKR 241
           STCPLCRGTL  +SPGLAIENPVYGFEDSEETEESTEDG R  I TAQKAPES+I GEKR
Sbjct: 183 STCPLCRGTL--YSPGLAIENPVYGFEDSEETEESTEDGRRISIPTAQKAPESDINGEKR 242

Query: 242 MFSIRLGKFRNLN-NGGLRGLEKGEGET-SSSSLSARRCYSMGSYQYIVADSELQVALRT 301
           +FSIRLGKFRNLN NGG+RGLEKGEGET SSSSL ARRCYSMGSYQYIVADSELQVAL T
Sbjct: 243 VFSIRLGKFRNLNSNGGVRGLEKGEGETSSSSSLGARRCYSMGSYQYIVADSELQVALST 302

Query: 302 SNRIGGGSMRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQP 361
           SN            +RDS TGNCS VDDD+AEGKKI IGSKNESFSVSKIWLWSKK RQP
Sbjct: 303 SN------------LRDSHTGNCS-VDDDNAEGKKIKIGSKNESFSVSKIWLWSKKGRQP 349

Query: 362 SGSETHV 366
           SG++THV
Sbjct: 363 SGADTHV 349

BLAST of Lsi04G005290 vs. NCBI nr
Match: XP_038893199.1 (RING-H2 finger protein ATL46-like [Benincasa hispida])

HSP 1 Score: 632.5 bits (1630), Expect = 2.3e-177
Identity = 338/368 (91.85%), Postives = 346/368 (94.02%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M  VHPQTRDFLVSPS SPL   SSSSSLAYNSDY KQEP  P TSSSSKISPAVLLVI+
Sbjct: 1   MSSVHPQTRDFLVSPSTSPL-QPSSSSSLAYNSDYLKQEPIPP-TSSSSKISPAVLLVII 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG 120
           ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG
Sbjct: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG 120

Query: 121 LDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS 180
           LDQAFIDALPVFLYKEIVG+KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS
Sbjct: 121 LDQAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS 180

Query: 181 NSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKR 240
           NSTCPLCRGTL  HSP LAIENPVYGFE+SEETE STEDGRFGI TA KA ESEIIGE+R
Sbjct: 181 NSTCPLCRGTL--HSPVLAIENPVYGFEESEETEVSTEDGRFGIPTAHKASESEIIGERR 240

Query: 241 MFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSN 300
           +FSIRLGKFRNLNNGGLRGLEK EGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSN
Sbjct: 241 VFSIRLGKFRNLNNGGLRGLEKEEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSN 300

Query: 301 RIGGGSMRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSG 360
           R GGGSMRFVKVIRDSQ GNC + DDDDA+GKKINIGSKNESFSVSKIWLWSKKSRQPSG
Sbjct: 301 RNGGGSMRFVKVIRDSQIGNCPIDDDDDADGKKINIGSKNESFSVSKIWLWSKKSRQPSG 360

Query: 361 SETHVRAS 369
           S+THVRAS
Sbjct: 361 SDTHVRAS 364

BLAST of Lsi04G005290 vs. NCBI nr
Match: XP_008446237.1 (PREDICTED: RING-H2 finger protein ATL46 [Cucumis melo])

HSP 1 Score: 624.4 bits (1609), Expect = 6.3e-175
Identity = 331/363 (91.18%), Postives = 342/363 (94.21%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M  +HPQTRDFLVSPSISPLH    SSSLAY+ DYQK EPT P TSSSSKISPAVLLVIV
Sbjct: 1   MSLIHPQTRDFLVSPSISPLH----SSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIV 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSE-SSAFQRQLQQLFHLHDS 120
           ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPE+SE SSAFQRQLQQLFHLHDS
Sbjct: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPELSESSSAFQRQLQQLFHLHDS 120

Query: 121 GLDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL 180
           GLDQAFIDALPVFLYKEIVG+KEPFDCAVCLCEFSELDKLRLLP+CSHAFHIDCIDTWLL
Sbjct: 121 GLDQAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPSCSHAFHIDCIDTWLL 180

Query: 181 SNSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEK 240
           SNSTCPLCRGTLHI SP LAIENPVYGFEDSEETEESTE+GRFGILTAQKAPES+IIGEK
Sbjct: 181 SNSTCPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEK 240

Query: 241 RMFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTS 300
           R+FSIRLGKFRNLNNGGLRGLEKGEGETSSSSL+ARRCYSMGSYQYIVA+SELQVAL TS
Sbjct: 241 RVFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLNARRCYSMGSYQYIVAESELQVALHTS 300

Query: 301 NRIGGGSMRFVKVIRDSQTGNCSV-VDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQP 360
           NRI GGSMRFVKVIRDSQTGNC +  DDDDAE KKINIGSKNESFSVSKIWLWSKKSRQP
Sbjct: 301 NRISGGSMRFVKVIRDSQTGNCPIDHDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQP 359

Query: 361 SGS 362
             S
Sbjct: 361 CSS 359

BLAST of Lsi04G005290 vs. NCBI nr
Match: XP_004135477.1 (RING-H2 finger protein ATL46 [Cucumis sativus] >KGN51821.1 hypothetical protein Csa_008432 [Cucumis sativus])

HSP 1 Score: 621.3 bits (1601), Expect = 5.3e-174
Identity = 331/363 (91.18%), Postives = 340/363 (93.66%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M   HPQTRDF VSPSISPLH    SSSLAY+ DYQK EPT P TSSSSKISPAVLLVIV
Sbjct: 1   MSLSHPQTRDFPVSPSISPLH----SSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIV 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSE-SSAFQRQLQQLFHLHDS 120
           ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFP++SE SSAFQRQLQQLFHLHDS
Sbjct: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPDLSESSSAFQRQLQQLFHLHDS 120

Query: 121 GLDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL 180
           GLDQAFIDALPVFLYKEIVG+KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL
Sbjct: 121 GLDQAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLL 180

Query: 181 SNSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEK 240
           SNSTCPLCRGTLHI SP LAIENPVYGFEDSEETEESTE+GRFGILTAQKAPES+IIGEK
Sbjct: 181 SNSTCPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEK 240

Query: 241 RMFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTS 300
           R+FSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVA+SELQVAL TS
Sbjct: 241 RVFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVAESELQVALHTS 300

Query: 301 NRIGGGSMRFVKVIRDSQTGNCSV-VDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQP 360
           NRI GGSMRFVKVIRDSQTGNC +  DDDDAE KKINIGSKNESFSVSKIWLWSKKSRQP
Sbjct: 301 NRISGGSMRFVKVIRDSQTGNCPIDRDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQP 359

Query: 361 SGS 362
             S
Sbjct: 361 CSS 359

BLAST of Lsi04G005290 vs. NCBI nr
Match: KAA0034330.1 (RING-H2 finger protein ATL46 [Cucumis melo var. makuwa] >TYK15589.1 RING-H2 finger protein ATL46 [Cucumis melo var. makuwa])

HSP 1 Score: 588.6 bits (1516), Expect = 3.8e-164
Identity = 309/335 (92.24%), Postives = 320/335 (95.52%), Query Frame = 0

Query: 29  LAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSS 88
           +AY+ DYQK EPT P TSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSS
Sbjct: 21  VAYDIDYQKLEPTPPSTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSS 80

Query: 89  ISESNSNRFPEMSE-SSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGIKEPFDCA 148
           ISESNSNRFPE+SE SSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVG+KEPFDCA
Sbjct: 81  ISESNSNRFPELSESSSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGLKEPFDCA 140

Query: 149 VCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIHSPGLAIENPVYGF 208
           VCLCEFSELDKLRLLP+CSHAFHIDCIDTWLLSNSTCPLCRGTLHI SP LAIENPVYGF
Sbjct: 141 VCLCEFSELDKLRLLPSCSHAFHIDCIDTWLLSNSTCPLCRGTLHIQSPVLAIENPVYGF 200

Query: 209 EDSEETEESTEDGRFGILTAQKAPESEIIGEKRMFSIRLGKFRNLNNGGLRGLEKGEGET 268
           EDSEETEESTE+GRFGILTAQKAPES+IIGEKR+FSIRLGKFRNLNNGGLRGLEKGEGET
Sbjct: 201 EDSEETEESTENGRFGILTAQKAPESDIIGEKRVFSIRLGKFRNLNNGGLRGLEKGEGET 260

Query: 269 SSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIRDSQTGNCSV-VDD 328
           SSSSL+ARRCYSMGSYQYIVA+SELQVAL TSNRI GGSMRFVKVIRDSQTGNC +  DD
Sbjct: 261 SSSSLNARRCYSMGSYQYIVAESELQVALHTSNRISGGSMRFVKVIRDSQTGNCPIDHDD 320

Query: 329 DDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGS 362
           DDAE KKINIGSKNESFSVSKIWLWSKKSRQP  S
Sbjct: 321 DDAERKKINIGSKNESFSVSKIWLWSKKSRQPCSS 355

BLAST of Lsi04G005290 vs. NCBI nr
Match: XP_022151161.1 (RING-H2 finger protein ATL47 [Momordica charantia] >XP_022151162.1 RING-H2 finger protein ATL47 [Momordica charantia])

HSP 1 Score: 564.3 bits (1453), Expect = 7.7e-157
Identity = 304/368 (82.61%), Postives = 326/368 (88.59%), Query Frame = 0

Query: 1   MYFVHPQTRDFLVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIV 60
           M F+HPQTR FLV+PSISPLHSSS  +       YQKQ   TP TSSSSKISPAVLLVIV
Sbjct: 1   MSFLHPQTRVFLVTPSISPLHSSSPLA-------YQKQ--PTPPTSSSSKISPAVLLVIV 60

Query: 61  ILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG 120
           ILA+  FISG+LH LVR LVKQR S SSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG
Sbjct: 61  ILAILFFISGILHFLVRFLVKQR-SPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSG 120

Query: 121 LDQAFIDALPVFLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS 180
           LDQAFIDALPVFLY EIVG+KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS
Sbjct: 121 LDQAFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLS 180

Query: 181 NSTCPLCRGTLHIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKR 240
           NSTCPLCRGTL  +SPGLAIENP++GFEDSE TE S EDGR G+ TAQK PE+++ GEKR
Sbjct: 181 NSTCPLCRGTL--YSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKR 240

Query: 241 MFSIRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSN 300
           +FSIRLGKFRNLNNGG+RGLEKGEGETSSSSL ARRCYSMGSYQYIVADS+LQVALRTSN
Sbjct: 241 VFSIRLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSN 300

Query: 301 RIGGGSMRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSG 360
           R GG SMRFV+  RDSQTGN  +  DDDAEGKKINIGSKNESFSVSKIWLWSKK+RQP G
Sbjct: 301 RTGGRSMRFVRA-RDSQTGNSLI--DDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIG 353

Query: 361 SETHVRAS 369
           S+ H+RA+
Sbjct: 361 SDVHLRAN 353

BLAST of Lsi04G005290 vs. TAIR 10
Match: AT5G40250.1 (RING/U-box superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 8.1e-88
Identity = 199/344 (57.85%), Postives = 243/344 (70.64%), Query Frame = 0

Query: 40  PTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPE 99
           P+ P  SS ++ISPAVL VIVILA+  FISGLLHLLVR L+K  S+++S   S SNRFPE
Sbjct: 30  PSPPPPSSGNRISPAVLFVIVILAVLFFISGLLHLLVRFLIKHPSATAS---SRSNRFPE 89

Query: 100 MSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVG----------IKEPFDCAVC 159
           +S S A QRQLQQLFHL+DSGLDQAFIDALPVF YKEIVG           +EPFDCAVC
Sbjct: 90  ISTSDALQRQLQQLFHLNDSGLDQAFIDALPVFHYKEIVGSAGGGGGNGAAQEPFDCAVC 149

Query: 160 LCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIHSPGLAIENPVYGFED 219
           LCEFSE DKLRLLP CSHAFH++CIDTWL SNSTCPLCRGTL   SPG ++ENP++ F+D
Sbjct: 150 LCEFSEKDKLRLLPMCSHAFHLNCIDTWLQSNSTCPLCRGTL--FSPGFSMENPMFDFDD 209

Query: 220 SEETEES-TEDGRFGILTAQKAPE-SEIIGEKRMFSIRLGKFRNLNN-GGLRGLE-KGEG 279
             E EE  TE+G      +QK  E  EI+ EK +  +RLGKF+ L+N G  +G +    G
Sbjct: 210 IREDEEGVTENG------SQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGG 269

Query: 280 ETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIRDS-QTGNCSVV 339
           ETSSS+L ARRC+SMGSYQYI+ +SEL+V    ++R+     R     ++S QTGN S  
Sbjct: 270 ETSSSNLDARRCFSMGSYQYILGNSELKVPF-ANDRL----PRLKPQDKESEQTGNSSSE 329

Query: 340 DDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGSETHVRAS 369
           D+     KKIN  +K ESFSVSKIWLW KK +  S ++  + +S
Sbjct: 330 DN-----KKINTVAKGESFSVSKIWLWPKKDKFSSDAQRRLPSS 352

BLAST of Lsi04G005290 vs. TAIR 10
Match: AT1G23980.1 (RING/U-box superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.5e-83
Identity = 190/362 (52.49%), Postives = 242/362 (66.85%), Query Frame = 0

Query: 12  LVSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGL 71
           L + +++ +  SS+ S +  N        ++  +  +++ISP +L +IV+L++  FI  +
Sbjct: 11  LKNDNLNQISPSSAPSPITLNHQLTDSSSSSS-SGGNNRISPIILFIIVLLSVIFFICSI 70

Query: 72  LHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPV 131
           LHLLVR  +K++ S+ S S + SN+ PE S+S  +QRQLQQLFHLHDSGLDQA IDALPV
Sbjct: 71  LHLLVRYYLKKKRSNLSSSPNESNQNPEFSDSDTYQRQLQQLFHLHDSGLDQALIDALPV 130

Query: 132 FLYKEIVGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTL 191
           FLYKEI G KEPFDCAVCLCEFSE DKLRLLP CSHAFHIDCIDTWLLSNSTCPLCRGTL
Sbjct: 131 FLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHAFHIDCIDTWLLSNSTCPLCRGTL 190

Query: 192 HIHSPGLAIENPVYGFEDSEETEESTEDGRFGILTA--QKAPESEIIGEKRMFSIRLGKF 251
              S G   E P + F        + +DG  G+  +  QK  E+EI   KR+FS+RLGKF
Sbjct: 191 --FSLGHQFEYPDFNFGFF-----AGDDGGGGVRVSPVQKPAENEI--GKRVFSVRLGKF 250

Query: 252 RN---LNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGS 311
           R+   +NNG +  +  G GETSSSSL  RRC+SMGSYQYIVA+S+L VAL  +N      
Sbjct: 251 RSSNIVNNGEV--VVGGGGETSSSSLDNRRCFSMGSYQYIVAESDLVVALCPNNE----- 310

Query: 312 MRFVKVIRDSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWS-KKSRQPSG--SET 366
                           + ++ D EGKKIN+ SK ESFSVSKIW WS K+S+ P+   SET
Sbjct: 311 ---------------GLKNNKDVEGKKINMRSKGESFSVSKIWQWSNKRSKFPNNHPSET 340

BLAST of Lsi04G005290 vs. TAIR 10
Match: AT4G30400.1 (RING/U-box superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-55
Identity = 160/399 (40.10%), Postives = 210/399 (52.63%), Query Frame = 0

Query: 13  VSPSISPLHSSSSSSSLAYNSDYQKQEPTTPLTSS-----SSKISPAVLLVIVILALFLF 72
           V P I    +  S SSL        QEP   L SS     +SKISP++LL+I+IL++  F
Sbjct: 11  VFPEIKTTQNFLSPSSL-------PQEPPLSLRSSANFDLNSKISPSILLIIIILSIIFF 70

Query: 73  ISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFID 132
           ISGLLHLLVR L         ++ S+ +R       +A Q QLQQLFHLHDSG+DQ+FID
Sbjct: 71  ISGLLHLLVRFL---------LTPSSRDREDYFDNVTALQGQLQQLFHLHDSGVDQSFID 130

Query: 133 ALPVFLYKEIVGIKE-PFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPL 192
            LPVF YK I+G+K  PFDCAVCLCEF   DKLRLLP CSHAFH+DCIDTWLLS+STCPL
Sbjct: 131 TLPVFHYKSIIGLKNYPFDCAVCLCEFETEDKLRLLPKCSHAFHMDCIDTWLLSHSTCPL 190

Query: 193 CRGT----------------LHIHSPGLAIENPVYGFEDSEETEESTED-----GRFGIL 252
           CR +                L + S        + G  DS     + +D        G++
Sbjct: 191 CRSSLLSDLSSHQDPRSSFLLVLESASDHSSREIGGDRDSAACVAANDDIDVSSAHLGLV 250

Query: 253 TAQKAPESEI----------------IGEKRMFSIRLGKFRNLNNGGLRGLEKGEGETSS 312
                  + I                +G+   FS++LGKFRN++ G   G        +S
Sbjct: 251 GNNDLGSTRIDSGHGDQYLDGELGGSVGKVVPFSVKLGKFRNIDIG--EGTSSNNNIGNS 310

Query: 313 SSLSARRCYSMGSYQYIV-ADSELQVALRT-----------SNRIGGGSMRFVKVIRDSQ 357
           SSL  RRC+SMGSY+YI+  ++ L+V + T            +R       F    R   
Sbjct: 311 SSLDERRCFSMGSYEYIMDEETTLKVHVSTKKQSSKNRGLPGHRTAMSECGFDPTGRLKF 370

BLAST of Lsi04G005290 vs. TAIR 10
Match: AT2G18650.1 (RING/U-box superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 5.5e-52
Identity = 139/347 (40.06%), Postives = 199/347 (57.35%), Query Frame = 0

Query: 42  TPLTSSSSKISPAVLLVIVILALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMS 101
           T L++ +SKI+P +LL+I+IL++  FISGLLH+LV+ L         ++ S  +R     
Sbjct: 30  TSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFL---------LTPSRESREDYFD 89

Query: 102 ESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYKEIVGIK-EPFDCAVCLCEFSELDKLR 161
             +A Q QLQQLF+LHDSG+DQ+ ID LPVF YK IVG+K  PFDC VCLCEF   DKLR
Sbjct: 90  NVTALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFETEDKLR 149

Query: 162 LLPTCSHAFHIDCIDTWLLSNSTCPLCRGTL--------HIHSPGLAI------------ 221
           LLP CSHAFH++CIDTWLLS+STCPLCR  L        ++ S  L +            
Sbjct: 150 LLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQSSRDMVP 209

Query: 222 ---ENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKRM-FSIRLGKFRNLNNGG 281
               N   G++ + ++E +    R G  +     + + + EK +   ++LGKFRN+++ G
Sbjct: 210 VLESNSQLGYDVNNDSESTRI--RSGRKSCDPDGDMDGLDEKVVPLEVKLGKFRNIDHVG 269

Query: 282 LRGLEKGEGETS--SSSLSARRCYSMGSYQYIVADSELQVALRTSNRIGGGSMRFVKVIR 341
             G ++ +   S  S ++  RRC SMGSY+YI+ D E  + +  S +   G  R    + 
Sbjct: 270 -EGSDQKKNSISGNSKNVDGRRCLSMGSYEYIM-DQEATLKVHVSTKKLSGKDR----VP 329

Query: 342 DSQTGNCSVVDDDDAEGKKINIGSKNESFSVSKIWLWSKKSRQPSGS 362
             +T       D   +G + ++  + ESFS+SKIWL  KK +Q   S
Sbjct: 330 SHRTVMSECGFDPTVKGIEKSV-VERESFSLSKIWLRGKKEKQKGTS 358

BLAST of Lsi04G005290 vs. TAIR 10
Match: AT3G48030.1 (hypoxia-responsive family protein / zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.3e-42
Identity = 121/289 (41.87%), Postives = 165/289 (57.09%), Query Frame = 0

Query: 16  SISPLHSSSSSSSLAYNSDYQKQEPTTPLTSSSSKISPAVLLVIVILALFLFISGLLHLL 75
           ++SP  SS+++ +L    D            SSS I   + LVI  LAL +   G+L+L+
Sbjct: 92  ALSPKSSSAATLTLMNQKD-----------PSSSSIVSVLCLVISGLALIIVFLGVLYLI 151

Query: 76  VRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYK 135
            + L   R SS+     + N  P++   S+   QLQ LF LHDSGLDQ  IDALPVFLY 
Sbjct: 152 FKFL---RKSSTLFPIPHFNYNPDLFSFSS--PQLQHLFFLHDSGLDQTAIDALPVFLYG 211

Query: 136 EI-VGIKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLHIH 195
            + + +++PFDCAVCL EFS+ DKLRLLP CSHAFH+ CIDTWLLSNSTCPLCR +    
Sbjct: 212 NVTISLEQPFDCAVCLNEFSDTDKLRLLPVCSHAFHLHCIDTWLLSNSTCPLCRRS---- 271

Query: 196 SPGLAIENPVYGFEDSEETEESTEDGRFGILTAQKAPESEIIGEKRMFSIRLGKFRNLNN 255
              L+  N  Y   +  ET  +   G       Q+  + +    KR+FS+RLG+F++ N 
Sbjct: 272 ---LSTSNVCY---NHSETLVAPLSGH------QQVDDGKASLAKRVFSVRLGRFKSTNE 331

Query: 256 GGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRIG 304
                 ++ + +        RRCYSMG+ QY+V D +  VAL +S R G
Sbjct: 332 S---QSQRHDVKDEIGVRMPRRCYSMGTQQYLVCDQDFVVALSSSPREG 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FL071.1e-8657.85RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1[more]
Q8GW384.9e-8252.49RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1[more]
Q940Q41.5e-5440.10RING-H2 finger protein ATL13 OS=Arabidopsis thaliana OX=3702 GN=ATL13 PE=2 SV=2[more]
Q9ZV537.7e-5140.06Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 P... [more]
Q7X8431.9e-4141.87RING-H2 finger protein ATL48 OS=Arabidopsis thaliana OX=3702 GN=ATL48 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3BE303.0e-17591.18RING-H2 finger protein ATL46 OS=Cucumis melo OX=3656 GN=LOC103489027 PE=4 SV=1[more]
A0A0A0KVN92.6e-17491.18RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G602200 P... [more]
A0A5D3CZH71.8e-16492.24RING-H2 finger protein ATL46 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1DCR23.7e-15782.61RING-H2 finger protein ATL47 OS=Momordica charantia OX=3673 GN=LOC111019155 PE=4... [more]
A0A6J1JJB02.3e-15484.20RING-H2 finger protein ATL46-like OS=Cucurbita maxima OX=3661 GN=LOC111486314 PE... [more]
Match NameE-valueIdentityDescription
XP_038893199.12.3e-17791.85RING-H2 finger protein ATL46-like [Benincasa hispida][more]
XP_008446237.16.3e-17591.18PREDICTED: RING-H2 finger protein ATL46 [Cucumis melo][more]
XP_004135477.15.3e-17491.18RING-H2 finger protein ATL46 [Cucumis sativus] >KGN51821.1 hypothetical protein ... [more]
KAA0034330.13.8e-16492.24RING-H2 finger protein ATL46 [Cucumis melo var. makuwa] >TYK15589.1 RING-H2 fing... [more]
XP_022151161.17.7e-15782.61RING-H2 finger protein ATL47 [Momordica charantia] >XP_022151162.1 RING-H2 finge... [more]
Match NameE-valueIdentityDescription
AT5G40250.18.1e-8857.85RING/U-box superfamily protein [more]
AT1G23980.13.5e-8352.49RING/U-box superfamily protein [more]
AT4G30400.11.1e-5540.10RING/U-box superfamily protein [more]
AT2G18650.15.5e-5240.06RING/U-box superfamily protein [more]
AT3G48030.11.3e-4241.87hypoxia-responsive family protein / zinc finger (C3HC4-type RING finger) family ... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 146..187
e-value: 1.5E-6
score: 37.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 145..188
e-value: 3.9E-12
score: 46.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 146..188
score: 12.297489
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 106..193
e-value: 6.2E-20
score: 72.7
NoneNo IPR availablePANTHERPTHR45768E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKEcoord: 13..366
NoneNo IPR availablePANTHERPTHR45768:SF59TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 13..366
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 145..188
e-value: 1.91761E-19
score: 78.8829
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 125..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G005290.1Lsi04G005290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0031625 ubiquitin protein ligase binding