Lsi04G000380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G000380
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAnnexin
Locationchr04: 540973 .. 543785 (+)
RNA-Seq ExpressionLsi04G000380
SyntenyLsi04G000380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAAAAGTTAATGCCAATAATAAATAAATAACACAGTTGATCCTAAATGAGTGGGGAGCCACCGTTGTAGGCCACGTCGAGCAATCTCCGGTGGAGGCGTCGATTTCAAGACGAGAGCTGGTAAATTCAAACAGAAGTGTAGAATTTTTAGTATTTAAAGGAGAGGGCAGAGACAGAGTGAGAGCATTCCGCAGCATAATCAGCACATCCCAACTCATAAATCCATCCAAACTTTCAAGTTATGGCCACCCTTCTTGTTCCTCACGACGTTCCTCCCCCAAATGTTGATGCAGAAGCCATTAAAGCCGCTTTCCGAGGTCATTTCATCCATCTATCTTTCATATTCATCACCGTTGTGTTCTGTTCTGATCGGATCATGTTTAGCTTTGCGAACCCTTGATGATTCTGAATCTTTCGTTCTTTCTCTTTCTAGGGTGGGGGACTGATGAGAAGGCCATCGTTGCCGTGCTAGGCTACAGAAATGCAGGTCAGAGGAGGCAAATCAGGATTGCTTATGAACAGCTTTTCGAAGAGGATCTCGTTAAGCGCTTTGAATCTGAGCTCTCTGGCCACCTCGAGGTTCGTCTTCGTCTTCATCATATTCTTGCCCATTACTCTCTAATCTCTATAGATCGTCGTCTTTATCATCAGTGTCAGATATTGCTTCTCTTGTTCGCGATCATCGAAACCACTGGATTCTCTACCCTTTTATAAAAGAAGATCGACCGATTTTATTGTTTATACGATTTTTGTAGAATATGAGGGTTTGATTGTTTGTTGTGGAAACAGAGAGCTGTGTACCGATGGATACTAGATCCAGAGGACAGAGACGCCGTGTTGGCGCACGTCGCCCTAAGGAAGCCCACTGAAGATTTTGCAGTGCTTGTTGAATTTTCTTGCATTTACTCTCCTGAAGAGTTCTTGGGGGTCAGAAGGGCGTATCAACACCGGTACAAGCGATCATTAGAGGAAGATGTTGCCGCCAACACTCACGATGATTTTCGCAAGGTCCGTAACTTGTCCCTTGTCCATTATATTAACTGGATTACCAATTGATCACGATTTCCTTTGTCGATCGTCTATATTTGGATCGCTGAGCTTAGGGTTACAGATCAACTTTTCAGTTTTGCTTTTGGGGTTCAGATCGTTTTAGTTACTTTCGAGAATAATTTTACCATTAATGTCTGAGTTCGATGATTTCAATACTCCAAGAGAGAGGATCTTCCTTCCCATTGAATAGAAAGATTTTATCCCACGTTTTGAACTGAGAAGAAATTCTAATCATGGGCAAAACCCAGTATTGAAAATGAAATGAAAAAACAGAAAAAGTTAAAGATCTGATGTATTGGGGGAATTCGAAACTGTTAAGAGGCTAAAATGAAATGATGTAACTTGAATATAGAGATTCTACCGACACAAGACTTTAAATAGTTAATGATCTATAAGGTAAGGGAATATCAGGGTAACTAGAATGACATGAACAAACTTAACTCGAGAATGAAGATAATAGTTTTTGTGAATTGTTGATTGGTGATTTATGATAAATTGTTGCAGCTGTTGGTGGGATTAGTGAGTGCATATCGTTACAGTGGTGGAGAGATAGATGCAAGACTAGTCAAATCAGAAGCTGAGATACTTGAGCGTGCAGTTAAAGACAAAACCTTCAATCATGAAGATGTCATAAGGATCCTAACCACAAGGAGTAAGGCACAGCTGATTGCAACTTTCAATCACTACAAAGATGCCAATGGTATTTCTATCTCTAAGGTATAAAGCTGACAGTAAAGATACTAGATTACCATTGATATTAATGTCGATCATATTCCTTTTGAGACATTAAGACTAAACCAATTGGATAGTTCACCACATTTTAGTATTGCTTATTAATTTACCATGAGAGTTTAAACCAAAACATCTTTGAAGCTGGATCGTGTTGAACTATAAGTTGGTATCCCTTTTTTCTGTTGCAGCAATTGGGTCAAGACCATGCTGCCAATGAGTTCACAGAAGCACTTAAAACTGTGATTCGATGCATCAATGACCCTATCAAATACTATGAGAAGGTGATGAATGAAACTCTTATCAAAGAAGATTATGTACTTCATTCATGTGCATGTTTTAATGCGTTTCTCTATTCCAATGCTTGCATTCGAATCTTCACTAATGAGCTGAAAAAGCTTTCCATCTCTTGATGTTGAAGCTATGCAAATTATTTCAACATTCCCATCTTGATTTACTGCGTGAACTCTATTTGTCCAATAATGCATCCATTTCTAAGAGAAGCGGAGATCAATAGTTCGATAAAATTGGAACGATATAGAGAAGATTAATAGTTGTTCTGAAAAAAACGGTTACAGGTGGTGCGAAATGCAATCAAGAAGGTTGGGAAGAGTGATGAGGATGCATTGACTCGCGTGATGGTGACAAGGGCGGAGAAAGACTTGAGGCAGATAAAGGAGGCTTATCACAAGAGAAATAGTGTTACCCTTGATGATGCTGTGTCCAAGGAGACCTCTGGCGACTACAAGCACTTCATCCTTGCTCTTCTGGGTAATAAAGCTGAGTGAAAACAGTGCTTCTTGAATCCATAATAAAATATATTACCTATGAGTGTTTTAATCGGCTTGTAAGTAGTTTTATTATATAATAAGCTGTTTATGCTTTGAGACTTGCGTTTGCTGAAGAATGCTTGGTTTTGTAATGAGGACATTTTTATAGTACTTAAGCTGTGTGTTATAAATTTCTCTGTTGCTGAGCTCCCCTGTTTTTCCTCTGTTTTCATTTACATCTCTCTCTCTTCCTTTATTTGATT

mRNA sequence

CTGAAAAGTTAATGCCAATAATAAATAAATAACACAGTTGATCCTAAATGAGTGGGGAGCCACCGTTGTAGGCCACGTCGAGCAATCTCCGGTGGAGGCGTCGATTTCAAGACGAGAGCTGGTAAATTCAAACAGAAGTGTAGAATTTTTAGTATTTAAAGGAGAGGGCAGAGACAGAGTGAGAGCATTCCGCAGCATAATCAGCACATCCCAACTCATAAATCCATCCAAACTTTCAAGTTATGGCCACCCTTCTTGTTCCTCACGACGTTCCTCCCCCAAATGTTGATGCAGAAGCCATTAAAGCCGCTTTCCGAGGGTGGGGGACTGATGAGAAGGCCATCGTTGCCGTGCTAGGCTACAGAAATGCAGGTCAGAGGAGGCAAATCAGGATTGCTTATGAACAGCTTTTCGAAGAGGATCTCGTTAAGCGCTTTGAATCTGAGCTCTCTGGCCACCTCGAGAGAGCTGTGTACCGATGGATACTAGATCCAGAGGACAGAGACGCCGTGTTGGCGCACGTCGCCCTAAGGAAGCCCACTGAAGATTTTGCAGTGCTTGTTGAATTTTCTTGCATTTACTCTCCTGAAGAGTTCTTGGGGGTCAGAAGGGCGTATCAACACCGGTACAAGCGATCATTAGAGGAAGATGTTGCCGCCAACACTCACGATGATTTTCGCAAGCTGTTGGTGGGATTAGTGAGTGCATATCGTTACAGTGGTGGAGAGATAGATGCAAGACTAGTCAAATCAGAAGCTGAGATACTTGAGCGTGCAGTTAAAGACAAAACCTTCAATCATGAAGATGTCATAAGGATCCTAACCACAAGGAGTAAGGCACAGCTGATTGCAACTTTCAATCACTACAAAGATGCCAATGGTATTTCTATCTCTAAGCAATTGGGTCAAGACCATGCTGCCAATGAGTTCACAGAAGCACTTAAAACTGTGATTCGATGCATCAATGACCCTATCAAATACTATGAGAAGGTGGTGCGAAATGCAATCAAGAAGGTTGGGAAGAGTGATGAGGATGCATTGACTCGCGTGATGGTGACAAGGGCGGAGAAAGACTTGAGGCAGATAAAGGAGGCTTATCACAAGAGAAATAGTGTTACCCTTGATGATGCTGTGTCCAAGGAGACCTCTGGCGACTACAAGCACTTCATCCTTGCTCTTCTGGGTAATAAAGCTGAGTGAAAACAGTGCTTCTTGAATCCATAATAAAATATATTACCTATGAGTGTTTTAATCGGCTTGTAAGTAGTTTTATTATATAATAAGCTGTTTATGCTTTGAGACTTGCGTTTGCTGAAGAATGCTTGGTTTTGTAATGAGGACATTTTTATAGTACTTAAGCTGTGTGTTATAAATTTCTCTGTTGCTGAGCTCCCCTGTTTTTCCTCTGTTTTCATTTACATCTCTCTCTCTTCCTTTATTTGATT

Coding sequence (CDS)

ATGGCCACCCTTCTTGTTCCTCACGACGTTCCTCCCCCAAATGTTGATGCAGAAGCCATTAAAGCCGCTTTCCGAGGGTGGGGGACTGATGAGAAGGCCATCGTTGCCGTGCTAGGCTACAGAAATGCAGGTCAGAGGAGGCAAATCAGGATTGCTTATGAACAGCTTTTCGAAGAGGATCTCGTTAAGCGCTTTGAATCTGAGCTCTCTGGCCACCTCGAGAGAGCTGTGTACCGATGGATACTAGATCCAGAGGACAGAGACGCCGTGTTGGCGCACGTCGCCCTAAGGAAGCCCACTGAAGATTTTGCAGTGCTTGTTGAATTTTCTTGCATTTACTCTCCTGAAGAGTTCTTGGGGGTCAGAAGGGCGTATCAACACCGGTACAAGCGATCATTAGAGGAAGATGTTGCCGCCAACACTCACGATGATTTTCGCAAGCTGTTGGTGGGATTAGTGAGTGCATATCGTTACAGTGGTGGAGAGATAGATGCAAGACTAGTCAAATCAGAAGCTGAGATACTTGAGCGTGCAGTTAAAGACAAAACCTTCAATCATGAAGATGTCATAAGGATCCTAACCACAAGGAGTAAGGCACAGCTGATTGCAACTTTCAATCACTACAAAGATGCCAATGGTATTTCTATCTCTAAGCAATTGGGTCAAGACCATGCTGCCAATGAGTTCACAGAAGCACTTAAAACTGTGATTCGATGCATCAATGACCCTATCAAATACTATGAGAAGGTGGTGCGAAATGCAATCAAGAAGGTTGGGAAGAGTGATGAGGATGCATTGACTCGCGTGATGGTGACAAGGGCGGAGAAAGACTTGAGGCAGATAAAGGAGGCTTATCACAAGAGAAATAGTGTTACCCTTGATGATGCTGTGTCCAAGGAGACCTCTGGCGACTACAAGCACTTCATCCTTGCTCTTCTGGGTAATAAAGCTGAGTGA

Protein sequence

MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEEDLVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLGVRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVKDKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCINDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKETSGDYKHFILALLGNKAE
Homology
BLAST of Lsi04G000380 vs. ExPASy Swiss-Prot
Match: P51074 (Annexin-like protein RJ4 OS=Fragaria ananassa OX=3747 PE=2 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 6.8e-112
Identity = 207/320 (64.69%), Postives = 260/320 (81.25%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNV----DAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQL 60
           MATL     V PPN     DAEA++ + +GWGT+EKAI+++LG+RNAGQR++IR AYEQL
Sbjct: 1   MATL-----VSPPNFCAKEDAEALRKSVKGWGTNEKAIISILGHRNAGQRKEIRAAYEQL 60

Query: 61  FEEDLVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPE 120
           ++EDL+K  ESELSG  E+AVYRW LDP DRDAVLA+VA++K T+ + V++E SCI+SPE
Sbjct: 61  YQEDLLKPLESELSGDFEKAVYRWTLDPADRDAVLANVAIKKSTDVYNVIIEISCIHSPE 120

Query: 121 EFLGVRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILE 180
           E L VRRAYQ RYK S+EED+AA+T  D RKLLV LV+AYRY G EI+A+L  SEA+IL 
Sbjct: 121 ELLAVRRAYQLRYKHSVEEDLAAHTTGDIRKLLVALVTAYRYDGHEINAKLANSEADILH 180

Query: 181 RAVKDKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTV 240
            A+KDK FNHE++IRIL+TRSK QL+ATFN Y+D  GISISK L  +  AN+F +AL T 
Sbjct: 181 DAIKDKAFNHEEIIRILSTRSKTQLMATFNKYRDDQGISISKNL-LEEGANDFQKALHTA 240

Query: 241 IRCINDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDA 300
           IRC+NDP KY+EKV+RNAIK+VG +DEDALTRV+VTRAE+DLR IKE Y+K+NSV L+ A
Sbjct: 241 IRCLNDPKKYFEKVLRNAIKRVG-TDEDALTRVIVTRAERDLRDIKEVYYKKNSVPLEQA 300

Query: 301 VSKETSGDYKHFILALLGNK 317
           V+K+TSGDYK F+L LLG +
Sbjct: 301 VAKDTSGDYKAFLLTLLGKE 313

BLAST of Lsi04G000380 vs. ExPASy Swiss-Prot
Match: Q94CK4 (Annexin D8 OS=Arabidopsis thaliana OX=3702 GN=ANNAT8 PE=2 SV=2)

HSP 1 Score: 339.7 bits (870), Expect = 3.5e-92
Identity = 175/315 (55.56%), Postives = 231/315 (73.33%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MAT++ P    P   DAE IKAA +GWGT+E AI+++LG+RN  QR+ IR AY++++ ED
Sbjct: 1   MATIVSPPHFSPVE-DAENIKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIYHED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+ + +SELSG+ ERA+  W+LDP +RDA+LA++AL+KP  D+ VLVE +C+ SPE+ L 
Sbjct: 61  LIHQLKSELSGNFERAICLWVLDPPERDALLANLALQKPIPDYKVLVEIACMRSPEDMLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
            RRAY+  YK SLEED+A+ T  D R+LLV +VSAY+Y G EID  L +SEA IL   + 
Sbjct: 121 ARRAYRCLYKHSLEEDLASRTIGDIRRLLVAMVSAYKYDGEEIDEMLAQSEAAILHDEIL 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
            K  +HE+ IR+L+TRS  QL A FN YKD  G SI+K L  +H  NE+  AL+  IRCI
Sbjct: 181 GKAVDHEETIRVLSTRSSMQLSAIFNRYKDIYGTSITKDL-LNHPTNEYLSALRAAIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
            +P +YY KV+RN+I  VG +DEDAL RV+VTRAEKDL  I   Y KRN+V+LD A++KE
Sbjct: 241 KNPTRYYAKVLRNSINTVG-TDEDALNRVIVTRAEKDLTNITGLYFKRNNVSLDQAIAKE 300

Query: 301 TSGDYKHFILALLGN 316
           TSGDYK F+LALLG+
Sbjct: 301 TSGDYKAFLLALLGH 312

BLAST of Lsi04G000380 vs. ExPASy Swiss-Prot
Match: Q9LX07 (Annexin D7 OS=Arabidopsis thaliana OX=3702 GN=ANNAT7 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-82
Identity = 163/315 (51.75%), Postives = 219/315 (69.52%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MA+L VP  VP P  DAE +  AF+GWGT+E+ I+++L +RNA QR  IR  Y   + +D
Sbjct: 1   MASLKVPATVPLPEEDAEQLYKAFKGWGTNERMIISILAHRNATQRSFIRAVYAANYNKD 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELSG  ERAV  W  +P +RDA LA  + +  T++  VLVE +C  S  E   
Sbjct: 61  LLKELDRELSGDFERAVMLWTFEPAERDAYLAKESTKMFTKNNWVLVEIACTRSALELFN 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
            ++AYQ RYK SLEEDVA +T  D RKLLV LVS +RY G E++  L +SEA+IL   +K
Sbjct: 121 AKQAYQARYKTSLEEDVAYHTSGDIRKLLVPLVSTFRYDGDEVNMTLARSEAKILHEKIK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           +K +  +D+IRILTTRSKAQ+ AT NHYK+  G S+SK L +D + NE+ + LK VI+C+
Sbjct: 181 EKAYADDDLIRILTTRSKAQISATLNHYKNNFGTSMSKYLKED-SENEYIQLLKAVIKCL 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
             P KY+EKV+R AI K+G +DE  LTRV+ TRAE D+ +IKE Y +RNSV LD A++K+
Sbjct: 241 TYPEKYFEKVLRQAINKLG-TDEWGLTRVVTTRAEFDMERIKEEYIRRNSVPLDRAIAKD 300

Query: 301 TSGDYKHFILALLGN 316
           T GDY+  +LALLG+
Sbjct: 301 THGDYEDILLALLGH 313

BLAST of Lsi04G000380 vs. ExPASy Swiss-Prot
Match: Q9XEE2 (Annexin D2 OS=Arabidopsis thaliana OX=3702 GN=ANN2 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-80
Identity = 155/315 (49.21%), Postives = 218/315 (69.21%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MA+L VP +VP P  DAE +  AF GWGT+EK I+++L +RNA QR  IR  Y   + ED
Sbjct: 1   MASLKVPSNVPLPEDDAEQLHKAFSGWGTNEKLIISILAHRNAAQRSLIRSVYAATYNED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELS   ERAV  W LDP +RDA LA  + +  T++  VLVE +C     E + 
Sbjct: 61  LLKALDKELSSDFERAVMLWTLDPPERDAYLAKESTKMFTKNNWVLVEIACTRPALELIK 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           V++AYQ RYK+S+EEDVA +T  D RKLL+ LVS +RY G +++  L +SEA+IL   V 
Sbjct: 121 VKQAYQARYKKSIEEDVAQHTSGDLRKLLLPLVSTFRYEGDDVNMMLARSEAKILHEKVS 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           +K+++ +D IRILTTRSKAQL AT NHY +  G +I+K L ++   N++ + L+ VI C+
Sbjct: 181 EKSYSDDDFIRILTTRSKAQLGATLNHYNNEYGNAINKNLKEESDDNDYMKLLRAVITCL 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
             P K++EKV+R +I K+G +DE  LTRV+ TR E D+ +IKE Y +RNS+ LD A++K+
Sbjct: 241 TYPEKHFEKVLRLSINKMG-TDEWGLTRVVTTRTEVDMERIKEEYQRRNSIPLDRAIAKD 300

Query: 301 TSGDYKHFILALLGN 316
           TSGDY+  ++ALLG+
Sbjct: 301 TSGDYEDMLVALLGH 314

BLAST of Lsi04G000380 vs. ExPASy Swiss-Prot
Match: Q9SYT0 (Annexin D1 OS=Arabidopsis thaliana OX=3702 GN=ANN1 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.2e-80
Identity = 153/314 (48.73%), Postives = 217/314 (69.11%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATL V   VP P+ DAE ++ AF GWGT+E  I+++L +R+A QR+ IR AY + + ED
Sbjct: 1   MATLKVSDSVPAPSDDAEQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELS   ERA+  W L+P +RDA+LA+ A ++ T    VL+E +C  +  + L 
Sbjct: 61  LLKTLDKELSNDFERAILLWTLEPGERDALLANEATKRWTSSNQVLMEVACTRTSTQLLH 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
            R+AY  RYK+SLEEDVA +T  DFRKLLV LV++YRY G E++  L K EA+++   +K
Sbjct: 121 ARQAYHARYKKSLEEDVAHHTTGDFRKLLVSLVTSYRYEGDEVNMTLAKQEAKLVHEKIK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK +N EDVIRIL+TRSKAQ+ ATFN Y+D +G  I K L +    ++F   L++ I+C+
Sbjct: 181 DKHYNDEDVIRILSTRSKAQINATFNRYQDDHGEEILKSLEEGDDDDKFLALLRSTIQCL 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
             P  Y+  V+R+AI K G +DE ALTR++ TRAE DL+ I E Y +RNS+ L+ A++K+
Sbjct: 241 TRPELYFVDVLRSAINKTG-TDEGALTRIVTTRAEIDLKVIGEEYQRRNSIPLEKAITKD 300

Query: 301 TSGDYKHFILALLG 315
           T GDY+  ++ALLG
Sbjct: 301 TRGDYEKMLVALLG 313

BLAST of Lsi04G000380 vs. ExPASy TrEMBL
Match: A0A1S3BG94 (annexin-like protein RJ4 OS=Cucumis melo OX=3656 GN=LOC103489497 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.4e-170
Identity = 306/318 (96.23%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDARL KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDARLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD AANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRAANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALLGNKAE 319
           TSGDYKHFILALLGN+ E
Sbjct: 301 TSGDYKHFILALLGNQTE 318

BLAST of Lsi04G000380 vs. ExPASy TrEMBL
Match: A0A0A0KRN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623690 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.0e-168
Identity = 303/318 (95.28%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFLG
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLG 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDA+L KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDAKLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD  ANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRDANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALLGNKAE 319
           TSGDY+ FILALLGN+ E
Sbjct: 301 TSGDYERFILALLGNQTE 318

BLAST of Lsi04G000380 vs. ExPASy TrEMBL
Match: E5GCK3 (Annexin OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.2e-167
Identity = 303/313 (96.81%), Postives = 306/313 (97.76%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDARL KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDARLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD AANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRAANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALL 314
           TSGDYKHFILALL
Sbjct: 301 TSGDYKHFILALL 313

BLAST of Lsi04G000380 vs. ExPASy TrEMBL
Match: A0A6J1GXE1 (annexin-like protein RJ4 OS=Cucurbita moschata OX=3662 GN=LOC111458366 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.9e-163
Identity = 294/315 (93.33%), Postives = 305/315 (96.83%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATL VPHDVPPPNVDAEAIKAAF+GWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED
Sbjct: 1   MATLRVPHDVPPPNVDAEAIKAAFKGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAV+VE SCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVIVELSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHD+FRKLLVGLVSAYRY+G EIDARL KSEA ILE+A+K
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDEFRKLLVGLVSAYRYNGQEIDARLAKSEAGILEKAIK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           +KTF+HEDVIRILTTRSKAQLIATFN YKDANGISISKQLGQD AANEFTEAL+TVIRCI
Sbjct: 181 EKTFHHEDVIRILTTRSKAQLIATFNSYKDANGISISKQLGQDRAANEFTEALRTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLR IKEAYHKRNSVTLDDAVSKE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRLIKEAYHKRNSVTLDDAVSKE 300

Query: 301 TSGDYKHFILALLGN 316
           TSGDYKHFILALLGN
Sbjct: 301 TSGDYKHFILALLGN 315

BLAST of Lsi04G000380 vs. ExPASy TrEMBL
Match: A0A6J1JTU1 (annexin-like protein RJ4 OS=Cucurbita maxima OX=3661 GN=LOC111489661 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.1e-163
Identity = 294/315 (93.33%), Postives = 305/315 (96.83%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATL VPHDVPPPNVDAEAIKAAF+GWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED
Sbjct: 1   MATLRVPHDVPPPNVDAEAIKAAFKGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGH+ERAVYRWILDPEDRDAVLAHVALRKP EDFAV+VE SCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHIERAVYRWILDPEDRDAVLAHVALRKPNEDFAVIVELSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHD+FRKLLVGLVSAYRY+G EIDARL KSEA ILE+A+K
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDEFRKLLVGLVSAYRYNGQEIDARLAKSEAGILEKAIK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           +KTF+HEDVIRILTTRSKAQLIATFN YKDANGISISKQLGQD AANEFTEAL+TVIRCI
Sbjct: 181 EKTFHHEDVIRILTTRSKAQLIATFNSYKDANGISISKQLGQDRAANEFTEALRTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDPIKYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLR IKEAYHKRNSVTLDDAVSKE
Sbjct: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRLIKEAYHKRNSVTLDDAVSKE 300

Query: 301 TSGDYKHFILALLGN 316
           TSGDYKHFILALLGN
Sbjct: 301 TSGDYKHFILALLGN 315

BLAST of Lsi04G000380 vs. NCBI nr
Match: XP_038892411.1 (annexin-like protein RJ4 [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 3.6e-171
Identity = 308/318 (96.86%), Postives = 314/318 (98.74%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAI+AVLGYRNAGQRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIIAVLGYRNAGQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+G EIDARL K+EAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGEEIDARLAKTEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD AANEFTEALKTVIRCI
Sbjct: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRAANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAVSKE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300

Query: 301 TSGDYKHFILALLGNKAE 319
           TSGDYKHFILALLGN+AE
Sbjct: 301 TSGDYKHFILALLGNQAE 318

BLAST of Lsi04G000380 vs. NCBI nr
Match: XP_008446932.1 (PREDICTED: annexin-like protein RJ4 [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 306/318 (96.23%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDARL KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDARLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD AANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRAANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALLGNKAE 319
           TSGDYKHFILALLGN+ E
Sbjct: 301 TSGDYKHFILALLGNQTE 318

BLAST of Lsi04G000380 vs. NCBI nr
Match: KGN52305.1 (hypothetical protein Csa_008521 [Cucumis sativus])

HSP 1 Score: 600.5 bits (1547), Expect = 8.4e-168
Identity = 303/318 (95.28%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFLG
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLG 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDA+L KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDAKLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD  ANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRDANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALLGNKAE 319
           TSGDY+ FILALLGN+ E
Sbjct: 301 TSGDYERFILALLGNQTE 318

BLAST of Lsi04G000380 vs. NCBI nr
Match: ADN34200.1 (annexin [Cucumis melo subsp. melo])

HSP 1 Score: 599.0 bits (1543), Expect = 2.4e-167
Identity = 303/313 (96.81%), Postives = 306/313 (97.76%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFL 
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDARL KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDARLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD AANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRAANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALL 314
           TSGDYKHFILALL
Sbjct: 301 TSGDYKHFILALL 313

BLAST of Lsi04G000380 vs. NCBI nr
Match: XP_004142392.1 (annexin A6 [Cucumis sativus])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 300/313 (95.85%), Postives = 305/313 (97.44%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNA QRRQIRIAYEQLFEED
Sbjct: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAPQRRQIRIAYEQLFEED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKP EDFAVLVEFSCIYSPEEFLG
Sbjct: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPNEDFAVLVEFSCIYSPEEFLG 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRY+GGEIDA+L KSEAEILERAVK
Sbjct: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYNGGEIDAKLAKSEAEILERAVK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK FNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQD  ANEFTEALKTVIRCI
Sbjct: 181 DKAFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDRDANEFTEALKTVIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
           NDP+KYYEKVVRNAIKKVGKSDEDALTRV+VTRAEKDLRQIKEAYHKRNSVTLDDAV KE
Sbjct: 241 NDPVKYYEKVVRNAIKKVGKSDEDALTRVVVTRAEKDLRQIKEAYHKRNSVTLDDAVKKE 300

Query: 301 TSGDYKHFILALL 314
           TSGDY+ FILALL
Sbjct: 301 TSGDYERFILALL 313

BLAST of Lsi04G000380 vs. TAIR 10
Match: AT5G12380.1 (annexin 8 )

HSP 1 Score: 339.7 bits (870), Expect = 2.5e-93
Identity = 175/315 (55.56%), Postives = 231/315 (73.33%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MAT++ P    P   DAE IKAA +GWGT+E AI+++LG+RN  QR+ IR AY++++ ED
Sbjct: 1   MATIVSPPHFSPVE-DAENIKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIYHED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+ + +SELSG+ ERA+  W+LDP +RDA+LA++AL+KP  D+ VLVE +C+ SPE+ L 
Sbjct: 61  LIHQLKSELSGNFERAICLWVLDPPERDALLANLALQKPIPDYKVLVEIACMRSPEDMLA 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
            RRAY+  YK SLEED+A+ T  D R+LLV +VSAY+Y G EID  L +SEA IL   + 
Sbjct: 121 ARRAYRCLYKHSLEEDLASRTIGDIRRLLVAMVSAYKYDGEEIDEMLAQSEAAILHDEIL 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
            K  +HE+ IR+L+TRS  QL A FN YKD  G SI+K L  +H  NE+  AL+  IRCI
Sbjct: 181 GKAVDHEETIRVLSTRSSMQLSAIFNRYKDIYGTSITKDL-LNHPTNEYLSALRAAIRCI 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
            +P +YY KV+RN+I  VG +DEDAL RV+VTRAEKDL  I   Y KRN+V+LD A++KE
Sbjct: 241 KNPTRYYAKVLRNSINTVG-TDEDALNRVIVTRAEKDLTNITGLYFKRNNVSLDQAIAKE 300

Query: 301 TSGDYKHFILALLGN 316
           TSGDYK F+LALLG+
Sbjct: 301 TSGDYKAFLLALLGH 312

BLAST of Lsi04G000380 vs. TAIR 10
Match: AT5G10230.1 (annexin 7 )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 163/315 (51.75%), Postives = 219/315 (69.52%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MA+L VP  VP P  DAE +  AF+GWGT+E+ I+++L +RNA QR  IR  Y   + +D
Sbjct: 1   MASLKVPATVPLPEEDAEQLYKAFKGWGTNERMIISILAHRNATQRSFIRAVYAANYNKD 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELSG  ERAV  W  +P +RDA LA  + +  T++  VLVE +C  S  E   
Sbjct: 61  LLKELDRELSGDFERAVMLWTFEPAERDAYLAKESTKMFTKNNWVLVEIACTRSALELFN 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
            ++AYQ RYK SLEEDVA +T  D RKLLV LVS +RY G E++  L +SEA+IL   +K
Sbjct: 121 AKQAYQARYKTSLEEDVAYHTSGDIRKLLVPLVSTFRYDGDEVNMTLARSEAKILHEKIK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           +K +  +D+IRILTTRSKAQ+ AT NHYK+  G S+SK L +D + NE+ + LK VI+C+
Sbjct: 181 EKAYADDDLIRILTTRSKAQISATLNHYKNNFGTSMSKYLKED-SENEYIQLLKAVIKCL 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
             P KY+EKV+R AI K+G +DE  LTRV+ TRAE D+ +IKE Y +RNSV LD A++K+
Sbjct: 241 TYPEKYFEKVLRQAINKLG-TDEWGLTRVVTTRAEFDMERIKEEYIRRNSVPLDRAIAKD 300

Query: 301 TSGDYKHFILALLGN 316
           T GDY+  +LALLG+
Sbjct: 301 THGDYEDILLALLGH 313

BLAST of Lsi04G000380 vs. TAIR 10
Match: AT5G65020.1 (annexin 2 )

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-81
Identity = 155/315 (49.21%), Postives = 218/315 (69.21%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MA+L VP +VP P  DAE +  AF GWGT+EK I+++L +RNA QR  IR  Y   + ED
Sbjct: 1   MASLKVPSNVPLPEDDAEQLHKAFSGWGTNEKLIISILAHRNAAQRSLIRSVYAATYNED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELS   ERAV  W LDP +RDA LA  + +  T++  VLVE +C     E + 
Sbjct: 61  LLKALDKELSSDFERAVMLWTLDPPERDAYLAKESTKMFTKNNWVLVEIACTRPALELIK 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
           V++AYQ RYK+S+EEDVA +T  D RKLL+ LVS +RY G +++  L +SEA+IL   V 
Sbjct: 121 VKQAYQARYKKSIEEDVAQHTSGDLRKLLLPLVSTFRYEGDDVNMMLARSEAKILHEKVS 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           +K+++ +D IRILTTRSKAQL AT NHY +  G +I+K L ++   N++ + L+ VI C+
Sbjct: 181 EKSYSDDDFIRILTTRSKAQLGATLNHYNNEYGNAINKNLKEESDDNDYMKLLRAVITCL 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
             P K++EKV+R +I K+G +DE  LTRV+ TR E D+ +IKE Y +RNS+ LD A++K+
Sbjct: 241 TYPEKHFEKVLRLSINKMG-TDEWGLTRVVTTRTEVDMERIKEEYQRRNSIPLDRAIAKD 300

Query: 301 TSGDYKHFILALLGN 316
           TSGDY+  ++ALLG+
Sbjct: 301 TSGDYEDMLVALLGH 314

BLAST of Lsi04G000380 vs. TAIR 10
Match: AT1G35720.1 (annexin 1 )

HSP 1 Score: 299.3 bits (765), Expect = 3.7e-81
Identity = 153/314 (48.73%), Postives = 217/314 (69.11%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MATL V   VP P+ DAE ++ AF GWGT+E  I+++L +R+A QR+ IR AY + + ED
Sbjct: 1   MATLKVSDSVPAPSDDAEQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGED 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELS   ERA+  W L+P +RDA+LA+ A ++ T    VL+E +C  +  + L 
Sbjct: 61  LLKTLDKELSNDFERAILLWTLEPGERDALLANEATKRWTSSNQVLMEVACTRTSTQLLH 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGGEIDARLVKSEAEILERAVK 180
            R+AY  RYK+SLEEDVA +T  DFRKLLV LV++YRY G E++  L K EA+++   +K
Sbjct: 121 ARQAYHARYKKSLEEDVAHHTTGDFRKLLVSLVTSYRYEGDEVNMTLAKQEAKLVHEKIK 180

Query: 181 DKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIRCI 240
           DK +N EDVIRIL+TRSKAQ+ ATFN Y+D +G  I K L +    ++F   L++ I+C+
Sbjct: 181 DKHYNDEDVIRILSTRSKAQINATFNRYQDDHGEEILKSLEEGDDDDKFLALLRSTIQCL 240

Query: 241 NDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVSKE 300
             P  Y+  V+R+AI K G +DE ALTR++ TRAE DL+ I E Y +RNS+ L+ A++K+
Sbjct: 241 TRPELYFVDVLRSAINKTG-TDEGALTRIVTTRAEIDLKVIGEEYQRRNSIPLEKAITKD 300

Query: 301 TSGDYKHFILALLG 315
           T GDY+  ++ALLG
Sbjct: 301 TRGDYEKMLVALLG 313

BLAST of Lsi04G000380 vs. TAIR 10
Match: AT5G10220.1 (annexin 6 )

HSP 1 Score: 298.9 bits (764), Expect = 4.8e-81
Identity = 158/317 (49.84%), Postives = 220/317 (69.40%), Query Frame = 0

Query: 1   MATLLVPHDVPPPNVDAEAIKAAFRGWGTDEKAIVAVLGYRNAGQRRQIRIAYEQLFEED 60
           MA+L +P ++P P  D+E +  AF+GWGT+E  I+++L +RNA QR  IR  Y   + +D
Sbjct: 1   MASLKIPANIPLPEEDSEQLHKAFKGWGTNEGMIISILAHRNATQRSFIRAVYAANYNKD 60

Query: 61  LVKRFESELSGHLERAVYRWILDPEDRDAVLAHVALRKPTEDFAVLVEFSCIYSPEEFLG 120
           L+K  + ELSG  ER V  W LDP +RDA LA+ + +  T++  VLVE +C     EF  
Sbjct: 61  LLKELDGELSGDFERVVMLWTLDPTERDAYLANESTKLFTKNIWVLVEIACTRPSLEFFK 120

Query: 121 VRRAYQHRYKRSLEEDVAANTHDDFRKLLVGLVSAYRYSGG--EIDARLVKSEAEILERA 180
            ++AY  RYK SLEEDVA +T  + RKLLV LVS +RY G   E++ +L +SEA+ L + 
Sbjct: 121 TKQAYHVRYKTSLEEDVAYHTSGNIRKLLVPLVSTFRYDGNADEVNVKLARSEAKTLHKK 180

Query: 181 VKDKTFNHEDVIRILTTRSKAQLIATFNHYKDANGISISKQLGQDHAANEFTEALKTVIR 240
           + +K +  ED+IRILTTRSKAQ+ AT NH+KD  G SI+K L +D + +++ + LKT I+
Sbjct: 181 ITEKAYTDEDLIRILTTRSKAQINATLNHFKDKFGSSINKFLKED-SNDDYVQLLKTAIK 240

Query: 241 CINDPIKYYEKVVRNAIKKVGKSDEDALTRVMVTRAEKDLRQIKEAYHKRNSVTLDDAVS 300
           C+  P KY+EKV+R AI ++G +DE ALTRV+ TRAE DL +IKE Y +RNSV LD A++
Sbjct: 241 CLTYPEKYFEKVLRRAINRMG-TDEWALTRVVTTRAEVDLERIKEEYLRRNSVPLDRAIA 300

Query: 301 KETSGDYKHFILALLGN 316
            +TSGDYK  +LALLG+
Sbjct: 301 NDTSGDYKDMLLALLGH 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P510746.8e-11264.69Annexin-like protein RJ4 OS=Fragaria ananassa OX=3747 PE=2 SV=2[more]
Q94CK43.5e-9255.56Annexin D8 OS=Arabidopsis thaliana OX=3702 GN=ANNAT8 PE=2 SV=2[more]
Q9LX071.9e-8251.75Annexin D7 OS=Arabidopsis thaliana OX=3702 GN=ANNAT7 PE=2 SV=1[more]
Q9XEE22.3e-8049.21Annexin D2 OS=Arabidopsis thaliana OX=3702 GN=ANN2 PE=1 SV=1[more]
Q9SYT05.2e-8048.73Annexin D1 OS=Arabidopsis thaliana OX=3702 GN=ANN1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BG947.4e-17096.23annexin-like protein RJ4 OS=Cucumis melo OX=3656 GN=LOC103489497 PE=4 SV=1[more]
A0A0A0KRN34.0e-16895.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623690 PE=4 SV=1[more]
E5GCK31.2e-16796.81Annexin OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A6J1GXE13.9e-16393.33annexin-like protein RJ4 OS=Cucurbita moschata OX=3662 GN=LOC111458366 PE=4 SV=1[more]
A0A6J1JTU15.1e-16393.33annexin-like protein RJ4 OS=Cucurbita maxima OX=3661 GN=LOC111489661 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038892411.13.6e-17196.86annexin-like protein RJ4 [Benincasa hispida][more]
XP_008446932.11.5e-16996.23PREDICTED: annexin-like protein RJ4 [Cucumis melo][more]
KGN52305.18.4e-16895.28hypothetical protein Csa_008521 [Cucumis sativus][more]
ADN34200.12.4e-16796.81annexin [Cucumis melo subsp. melo][more]
XP_004142392.11.3e-16595.85annexin A6 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G12380.12.5e-9355.56annexin 8 [more]
AT5G10230.11.4e-8351.75annexin 7 [more]
AT5G65020.11.7e-8149.21annexin 2 [more]
AT1G35720.13.7e-8148.73annexin 1 [more]
AT5G10220.14.8e-8149.84annexin 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001464AnnexinPRINTSPR00196ANNEXINcoord: 65..81
score: 34.53
coord: 174..200
score: 22.77
coord: 300..313
score: 48.02
coord: 256..276
score: 38.27
coord: 25..47
score: 43.88
IPR009118Annexin D, plantPRINTSPR01814ANNEXINPLANTcoord: 161..181
score: 36.85
coord: 119..133
score: 54.78
coord: 227..245
score: 34.1
IPR018502Annexin repeatSMARTSM00335annex3coord: 260..312
e-value: 4.2E-18
score: 76.1
coord: 183..236
e-value: 0.25
score: 6.9
coord: 100..152
e-value: 0.0083
score: 22.2
coord: 28..80
e-value: 1.3E-13
score: 61.2
IPR018502Annexin repeatPFAMPF00191Annexincoord: 171..223
e-value: 8.2E-11
score: 41.8
coord: 16..77
e-value: 6.8E-17
score: 61.3
coord: 250..312
e-value: 1.1E-16
score: 60.6
coord: 88..152
e-value: 3.0E-7
score: 30.4
IPR018502Annexin repeatPROSITEPS51897ANNEXIN_2coord: 166..238
score: 16.437859
IPR018502Annexin repeatPROSITEPS51897ANNEXIN_2coord: 83..154
score: 18.047907
IPR018502Annexin repeatPROSITEPS51897ANNEXIN_2coord: 11..82
score: 21.390676
IPR018502Annexin repeatPROSITEPS51897ANNEXIN_2coord: 242..314
score: 23.499073
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 83..153
e-value: 2.2E-13
score: 52.2
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 166..241
e-value: 1.7E-18
score: 68.5
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 242..317
e-value: 9.3E-21
score: 75.7
IPR037104Annexin superfamilyGENE3D1.10.220.10Annexincoord: 1..82
e-value: 2.8E-23
score: 83.9
IPR037104Annexin superfamilySUPERFAMILY47874Annexincoord: 2..314
NoneNo IPR availablePANTHERPTHR10502ANNEXINcoord: 10..316
NoneNo IPR availablePANTHERPTHR10502:SF204ANNEXINcoord: 10..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000380.1Lsi04G000380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006950 response to stress
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005544 calcium-dependent phospholipid binding
molecular_function GO:0005509 calcium ion binding