Lsi03G020440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G020440
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr03: 31779615 .. 31782413 (-)
RNA-Seq ExpressionLsi03G020440
SyntenyLsi03G020440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAGTAACATTATAATATAGTTGGGTTTTGGAACTGGAGCTGTACACATTTGGCACACAAGCTATTTTCTAATGTGAAACCGGATTGTAGAAATTTGAATGGAATTTCTGGTTTGACTTCCCAGATTTGAATGCCCTGATCTTGGCAGTCTCATATGTTCAATCATTCCACCCCATGGTTAGGGTTGTGTCCTATACAATCAGTCTCTAAGTCATACTAGAGATAGTGGTTGAGAACAAGCATGGTTGAAATTTCCATGTGGAGGTTGATGCTTGCAGTTGCAGGGGATGGATAAATTGAAAATGAAAAGCAGTTGAAGGGTTTTCTCTCTACATATTTATCCACCATTAATTTCTAAACCCCATGTGCTGATCTCTGCAGTGTGTTTAAGAATGGCATTAGAATAGATTACAAGAATGGCATTTATAAGAACCTTAAATTCCATATATTTTGTAACTTTAGCATCAGATGCTATTGTTTTCTTCAACCCTTTTAGTCCAACTACGTCAACAAAGAACTTCAAACCTCAACTGCATTTAGCACTCTCCCATATTCGACTCAATACCCAGCTTGCCTTTTCTCCCTTCCCTGTTACTGATCATTATTCTCATGATGATAAGTTAATCTCTCTCTGCAAGAAAAACTGTCACAGAGAAGCTCTTCAAGCATTTGACATCTTTCAAAAGTGTTCAAATTCTCCATTGAAGTCTATCACTTATACCCATTTAATCAACGCATGCTCTTCTCTAAGATCCCTAGAACATGGTAGGAAAATTCATCGGCATATGTTGACATTCAACTACCAGCCTGATATGATACTTCAGAATCATATTCTTAATATGTATGGAAAATGTGGGTCTTTGAAGGAAGCAAGAAATATTTTTGATGCAATGCCCCTAAAGAATGTAGTATCTTGGACCTCCATGATATCTGGCTACTCACATTATGGTCAAGAGGATAATGCCATTGCATTGTATGTTCAAATGTTACGATCAGGATATATTCCTGATCACTTTACCTTCGGAAGTGTAGTAAAGTCTTGCTCAGGACTTGATGATTTTATGTTAGCAAGGCAACTGCATGCCCATGTTCTGAAGTCTGAATTTGGCAGTCACCTAATTGCACAGAATGCTCTAATCTCTATGTATACCAAGTTAAGTCAAATGGCTGATGCAAAGAATGTCTTCTCTCATATTATTATAAAGGATCTAATTTCTTGGGGCTCAATGATTGCAGGGTTTTCTCAGCTTGGTTATGAGCTTGAAGCTTTATGCCATTTTAGGGAAATGCTTTCTCAGCCTGTCTATCAGCCAAATGAGTTTGTCTTTGGTAGTGCCTTCAGTGCTTGTAGCAAGCTCTTAGAACCAAATTATGGACGCCAAATACATGGCTTGTGTATAAAATTTGGGTTAGGAAGTGATCTTTTTGCTGGATGCTCCCTTTGTGACATGTATGCAAAGTGTGGATTTTTAGGATCTGCAAGAACAGTATTTTATCATATTGAAAAGCCTGATTTAGTAGCCTGGAATTCTATCATTGCAGGATTTGCTAGTGTTGGTGATGCAAAGGAATCCTTGTCATTCTTTTCTCGGATGAGGCATACAGGACTTGTTCCAAATGATGTCACCGTTCTCTCTTTACTTTGTGCTTGTTCAGAGCCTGTGATGCTTAATCAGGGAATGCAGGTTCACTCCTACATTGTCAGGATGGGCTTTGATTTAGATATTCCTGTGTGCAACAGTTTGCTTAGCATGTATTCAAAATGCTCAAATTTGAATGATGCACTTCAAATATTTGAAGGTATAGGAAACAAAGCTGATATAGTTTCTTGGAACACCATGCTTACGGCATGTCTCCAGCAGAACCAAGCTGGAGAGGTTCTAAGATTAACGAAGCTAATGCTTGCTTCTCACATCAAGCCTGACCATGTTACTTTAACTAATGTGTTGGTGTCATCCGGACAAATAGCTTCTTATGAAGTGGGAAGTCAAGTTCATTGCTTTATCATGAAATCAGGACTGAATCTTGATACTTCTGTTTCGAATGCGTTAATCAACATGTATACAAAGTGTGGATCCCTTGGTTGTGCTCGAAAGATGTTTGATTCCATTGACAATCCCGATATCATTTCATGGAGTAGCTTGATTGTTGGATATGCTCAGGCTGGATGCGGCGAAGAAGCTTTTGAGCTTTTTAGAACCATGAGAGGTCTCGGCGTAAAGCCAAATGAAATTACATTTGTAGGAATCCTTACTGCTTGTAGTCATATTGGAATGGTAGAAGAAGGTTTGAAGTTATACAGGACAATGCAAGAGGAATATGACATTTCACCAACCAAAGAACACTGTTCATGTATGGTCGACTTGCTCGCTCGTGCTGGATGCTTGGATGGAGCAGAGGACTTCATTAAGCACATGCCTTTTGATCCCGACATCGTGGTCTGGAAAACTCTGCTAGCAGCATGTAAAGTCCATGGTAATCTTGAGGTTGGCAAAAGGGCTGCAGAGAATGTATTAAAAATCGATCCATCAAACTCCGCTGCGCTCGTAATGCTTTGTAATATACATGCTTCTTCTGGCTGTTGGAAAGATTTCGCTCAATATAGAAGCTCAATGAGACAAATGAATGTCAGCAAAGTTCCAGGTCAGAGCTGGATTGAGATCAAGGATAAAGTTCATGTGTTTCTTGCAGAAGATAGCTTGCATCCTGAAAGAGGTAAAATTTACACAATGCTAGAGGAGTTAATGTTGCAAGTTCTAGATGACGGTTGTGATCCAATAAAGGTTACAAAATAG

mRNA sequence

ATGAACACATCAGATGCTATTGTTTTCTTCAACCCTTTTAGTCCAACTACGTCAACAAAGAACTTCAAACCTCAACTGCATTTAGCACTCTCCCATATTCGACTCAATACCCAGCTTGCCTTTTCTCCCTTCCCTGTTACTGATCATTATTCTCATGATGATAAGTTAATCTCTCTCTGCAAGAAAAACTGTCACAGAGAAGCTCTTCAAGCATTTGACATCTTTCAAAAGTGTTCAAATTCTCCATTGAAGTCTATCACTTATACCCATTTAATCAACGCATGCTCTTCTCTAAGATCCCTAGAACATGGTAGGAAAATTCATCGGCATATGTTGACATTCAACTACCAGCCTGATATGATACTTCAGAATCATATTCTTAATATGTATGGAAAATGTGGGTCTTTGAAGGAAGCAAGAAATATTTTTGATGCAATGCCCCTAAAGAATGTAGTATCTTGGACCTCCATGATATCTGGCTACTCACATTATGGTCAAGAGGATAATGCCATTGCATTGTATGTTCAAATGTTACGATCAGGATATATTCCTGATCACTTTACCTTCGGAAGTGTAGTAAAGTCTTGCTCAGGACTTGATGATTTTATGTTAGCAAGGCAACTGCATGCCCATGTTCTGAAGTCTGAATTTGGCAGTCACCTAATTGCACAGAATGCTCTAATCTCTATGTATACCAAGTTAAGTCAAATGGCTGATGCAAAGAATGTCTTCTCTCATATTATTATAAAGGATCTAATTTCTTGGGGCTCAATGATTGCAGGGTTTTCTCAGCTTGGTTATGAGCTTGAAGCTTTATGCCATTTTAGGGAAATGCTTTCTCAGCCTGTCTATCAGCCAAATGAGTTTGTCTTTGGTAGTGCCTTCAGTGCTTGTAGCAAGCTCTTAGAACCAAATTATGGACGCCAAATACATGGCTTGTGTATAAAATTTGGGTTAGGAAGTGATCTTTTTGCTGGATGCTCCCTTTGTGACATGTATGCAAAGTGTGGATTTTTAGGATCTGCAAGAACAGTATTTTATCATATTGAAAAGCCTGATTTAGTAGCCTGGAATTCTATCATTGCAGGATTTGCTAGTGTTGGTGATGCAAAGGAATCCTTGTCATTCTTTTCTCGGATGAGGCATACAGGACTTGTTCCAAATGATGTCACCGTTCTCTCTTTACTTTGTGCTTGTTCAGAGCCTGTGATGCTTAATCAGGGAATGCAGGTTCACTCCTACATTGTCAGGATGGGCTTTGATTTAGATATTCCTGTGTGCAACAGTTTGCTTAGCATGTATTCAAAATGCTCAAATTTGAATGATGCACTTCAAATATTTGAAGGTATAGGAAACAAAGCTGATATAGTTTCTTGGAACACCATGCTTACGGCATGTCTCCAGCAGAACCAAGCTGGAGAGGTTCTAAGATTAACGAAGCTAATGCTTGCTTCTCACATCAAGCCTGACCATGTTACTTTAACTAATGTGTTGGTGTCATCCGGACAAATAGCTTCTTATGAAGTGGGAAGTCAAGTTCATTGCTTTATCATGAAATCAGGACTGAATCTTGATACTTCTGTTTCGAATGCGTTAATCAACATGTATACAAAGTGTGGATCCCTTGGTTGTGCTCGAAAGATGTTTGATTCCATTGACAATCCCGATATCATTTCATGGAGTAGCTTGATTGTTGGATATGCTCAGGCTGGATGCGGCGAAGAAGCTTTTGAGCTTTTTAGAACCATGAGAGGTCTCGGCGTAAAGCCAAATGAAATTACATTTGTAGGAATCCTTACTGCTTGTAGTCATATTGGAATGGTAGAAGAAGGTTTGAAGTTATACAGGACAATGCAAGAGGAATATGACATTTCACCAACCAAAGAACACTGTTCATGTATGGTCGACTTGCTCGCTCGTGCTGGATGCTTGGATGGAGCAGAGGACTTCATTAAGCACATGCCTTTTGATCCCGACATCGTGGTCTGGAAAACTCTGCTAGCAGCATGTAAAGTCCATGGTAATCTTGAGGTTGGCAAAAGGGCTGCAGAGAATGTATTAAAAATCGATCCATCAAACTCCGCTGCGCTCGTAATGCTTTGTAATATACATGCTTCTTCTGGCTGTTGGAAAGATTTCGCTCAATATAGAAGCTCAATGAGACAAATGAATGTCAGCAAAGTTCCAGGTCAGAGCTGGATTGAGATCAAGGATAAAGTTCATGTGTTTCTTGCAGAAGATAGCTTGCATCCTGAAAGAGGTAAAATTTACACAATGCTAGAGGAGTTAATGTTGCAAGTTCTAGATGACGGTTGTGATCCAATAAAGGTTACAAAATAG

Coding sequence (CDS)

ATGAACACATCAGATGCTATTGTTTTCTTCAACCCTTTTAGTCCAACTACGTCAACAAAGAACTTCAAACCTCAACTGCATTTAGCACTCTCCCATATTCGACTCAATACCCAGCTTGCCTTTTCTCCCTTCCCTGTTACTGATCATTATTCTCATGATGATAAGTTAATCTCTCTCTGCAAGAAAAACTGTCACAGAGAAGCTCTTCAAGCATTTGACATCTTTCAAAAGTGTTCAAATTCTCCATTGAAGTCTATCACTTATACCCATTTAATCAACGCATGCTCTTCTCTAAGATCCCTAGAACATGGTAGGAAAATTCATCGGCATATGTTGACATTCAACTACCAGCCTGATATGATACTTCAGAATCATATTCTTAATATGTATGGAAAATGTGGGTCTTTGAAGGAAGCAAGAAATATTTTTGATGCAATGCCCCTAAAGAATGTAGTATCTTGGACCTCCATGATATCTGGCTACTCACATTATGGTCAAGAGGATAATGCCATTGCATTGTATGTTCAAATGTTACGATCAGGATATATTCCTGATCACTTTACCTTCGGAAGTGTAGTAAAGTCTTGCTCAGGACTTGATGATTTTATGTTAGCAAGGCAACTGCATGCCCATGTTCTGAAGTCTGAATTTGGCAGTCACCTAATTGCACAGAATGCTCTAATCTCTATGTATACCAAGTTAAGTCAAATGGCTGATGCAAAGAATGTCTTCTCTCATATTATTATAAAGGATCTAATTTCTTGGGGCTCAATGATTGCAGGGTTTTCTCAGCTTGGTTATGAGCTTGAAGCTTTATGCCATTTTAGGGAAATGCTTTCTCAGCCTGTCTATCAGCCAAATGAGTTTGTCTTTGGTAGTGCCTTCAGTGCTTGTAGCAAGCTCTTAGAACCAAATTATGGACGCCAAATACATGGCTTGTGTATAAAATTTGGGTTAGGAAGTGATCTTTTTGCTGGATGCTCCCTTTGTGACATGTATGCAAAGTGTGGATTTTTAGGATCTGCAAGAACAGTATTTTATCATATTGAAAAGCCTGATTTAGTAGCCTGGAATTCTATCATTGCAGGATTTGCTAGTGTTGGTGATGCAAAGGAATCCTTGTCATTCTTTTCTCGGATGAGGCATACAGGACTTGTTCCAAATGATGTCACCGTTCTCTCTTTACTTTGTGCTTGTTCAGAGCCTGTGATGCTTAATCAGGGAATGCAGGTTCACTCCTACATTGTCAGGATGGGCTTTGATTTAGATATTCCTGTGTGCAACAGTTTGCTTAGCATGTATTCAAAATGCTCAAATTTGAATGATGCACTTCAAATATTTGAAGGTATAGGAAACAAAGCTGATATAGTTTCTTGGAACACCATGCTTACGGCATGTCTCCAGCAGAACCAAGCTGGAGAGGTTCTAAGATTAACGAAGCTAATGCTTGCTTCTCACATCAAGCCTGACCATGTTACTTTAACTAATGTGTTGGTGTCATCCGGACAAATAGCTTCTTATGAAGTGGGAAGTCAAGTTCATTGCTTTATCATGAAATCAGGACTGAATCTTGATACTTCTGTTTCGAATGCGTTAATCAACATGTATACAAAGTGTGGATCCCTTGGTTGTGCTCGAAAGATGTTTGATTCCATTGACAATCCCGATATCATTTCATGGAGTAGCTTGATTGTTGGATATGCTCAGGCTGGATGCGGCGAAGAAGCTTTTGAGCTTTTTAGAACCATGAGAGGTCTCGGCGTAAAGCCAAATGAAATTACATTTGTAGGAATCCTTACTGCTTGTAGTCATATTGGAATGGTAGAAGAAGGTTTGAAGTTATACAGGACAATGCAAGAGGAATATGACATTTCACCAACCAAAGAACACTGTTCATGTATGGTCGACTTGCTCGCTCGTGCTGGATGCTTGGATGGAGCAGAGGACTTCATTAAGCACATGCCTTTTGATCCCGACATCGTGGTCTGGAAAACTCTGCTAGCAGCATGTAAAGTCCATGGTAATCTTGAGGTTGGCAAAAGGGCTGCAGAGAATGTATTAAAAATCGATCCATCAAACTCCGCTGCGCTCGTAATGCTTTGTAATATACATGCTTCTTCTGGCTGTTGGAAAGATTTCGCTCAATATAGAAGCTCAATGAGACAAATGAATGTCAGCAAAGTTCCAGGTCAGAGCTGGATTGAGATCAAGGATAAAGTTCATGTGTTTCTTGCAGAAGATAGCTTGCATCCTGAAAGAGGTAAAATTTACACAATGCTAGAGGAGTTAATGTTGCAAGTTCTAGATGACGGTTGTGATCCAATAAAGGTTACAAAATAG

Protein sequence

MNTSDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKVTK
Homology
BLAST of Lsi03G020440 vs. ExPASy Swiss-Prot
Match: Q9LFI1 (Pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E86 PE=2 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 3.1e-259
Identity = 426/722 (59.00%), Postives = 552/722 (76.45%), Query Frame = 0

Query: 53  DDKLISLCKKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHML 112
           +D + SLCK N +REAL+AFD  QK S+  ++  TY  LI ACSS RSL  GRKIH H+L
Sbjct: 35  NDHINSLCKSNFYREALEAFDFAQKNSSFKIRLRTYISLICACSSSRSLAQGRKIHDHIL 94

Query: 113 TFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIA 172
             N + D IL NHIL+MYGKCGSL++AR +FD MP +N+VS+TS+I+GYS  GQ   AI 
Sbjct: 95  NSNCKYDTILNNHILSMYGKCGSLRDAREVFDFMPERNLVSYTSVITGYSQNGQGAEAIR 154

Query: 173 LYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYT 232
           LY++ML+   +PD F FGS++K+C+   D  L +QLHA V+K E  SHLIAQNALI+MY 
Sbjct: 155 LYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGKQLHAQVIKLESSSHLIAQNALIAMYV 214

Query: 233 KLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFG 292
           + +QM+DA  VF  I +KDLISW S+IAGFSQLG+E EAL H +EMLS  V+ PNE++FG
Sbjct: 215 RFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLGFEFEALSHLKEMLSFGVFHPNEYIFG 274

Query: 293 SAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKP 352
           S+  ACS LL P+YG QIHGLCIK  L  +  AGCSLCDMYA+CGFL SAR VF  IE+P
Sbjct: 275 SSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAGCSLCDMYARCGFLNSARRVFDQIERP 334

Query: 353 DLVAWNSIIAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVH 412
           D  +WN IIAG A+ G A E++S FS+MR +G +P+ +++ SLLCA ++P+ L+QGMQ+H
Sbjct: 335 DTASWNVIIAGLANNGYADEAVSVFSQMRSSGFIPDAISLRSLLCAQTKPMALSQGMQIH 394

Query: 413 SYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQA 472
           SYI++ GF  D+ VCNSLL+MY+ CS+L     +FE   N AD VSWNT+LTACLQ  Q 
Sbjct: 395 SYIIKWGFLADLTVCNSLLTMYTFCSDLYCCFNLFEDFRNNADSVSWNTILTACLQHEQP 454

Query: 473 GEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNAL 532
            E+LRL KLML S  +PDH+T+ N+L    +I+S ++GSQVHC+ +K+GL  +  + N L
Sbjct: 455 VEMLRLFKLMLVSECEPDHITMGNLLRGCVEISSLKLGSQVHCYSLKTGLAPEQFIKNGL 514

Query: 533 INMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNE 592
           I+MY KCGSLG AR++FDS+DN D++SWS+LIVGYAQ+G GEEA  LF+ M+  G++PN 
Sbjct: 515 IDMYAKCGSLGQARRIFDSMDNRDVVSWSTLIVGYAQSGFGEEALILFKEMKSAGIEPNH 574

Query: 593 ITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIK 652
           +TFVG+LTACSH+G+VEEGLKLY TMQ E+ ISPTKEHCSC+VDLLARAG L+ AE FI 
Sbjct: 575 VTFVGVLTACSHVGLVEEGLKLYATMQTEHGISPTKEHCSCVVDLLARAGRLNEAERFID 634

Query: 653 HMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKD 712
            M  +PD+VVWKTLL+ACK  GN+ + ++AAEN+LKIDP NS A V+LC++HASSG W++
Sbjct: 635 EMKLEPDVVVWKTLLSACKTQGNVHLAQKAAENILKIDPFNSTAHVLLCSMHASSGNWEN 694

Query: 713 FAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGC 772
            A  RSSM++ +V K+PGQSWIEI+DK+H+F AED  HPER  IYT+L  +  Q+LD+ C
Sbjct: 695 AALLRSSMKKHDVKKIPGQSWIEIEDKIHIFFAEDIFHPERDDIYTVLHNIWSQMLDE-C 754

Query: 773 DP 775
           +P
Sbjct: 755 NP 755

BLAST of Lsi03G020440 vs. ExPASy Swiss-Prot
Match: Q9SS60 (Pentatricopeptide repeat-containing protein At3g03580 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H23 PE=2 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 1.4e-131
Identity = 237/730 (32.47%), Postives = 401/730 (54.93%), Query Frame = 0

Query: 45  PVTDHYSHDDKLISLCKKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHG 104
           P  + Y  +  + +  K     EAL+ +   ++   SP K  T+  +I AC+ L   E G
Sbjct: 67  PAKNVYLWNSIIRAFSKNGLFPEALEFYGKLRESKVSPDK-YTFPSVIKACAGLFDAEMG 126

Query: 105 RKIHRHMLTFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHY 164
             ++  +L   ++ D+ + N +++MY + G L  AR +FD MP++++VSW S+ISGYS +
Sbjct: 127 DLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSH 186

Query: 165 GQEDNAIALYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQ 224
           G  + A+ +Y ++  S  +PD FT  SV+ +   L      + LH   LKS   S ++  
Sbjct: 187 GYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQGLHGFALKSGVNSVVVVN 246

Query: 225 NALISMYTKLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVY 284
           N L++MY K  +  DA+ VF  + ++D +S+ +MI G+ +L    E++  F E L Q  +
Sbjct: 247 NGLVAMYLKFRRPTDARRVFDEMDVRDSVSYNTMICGYLKLEMVEESVRMFLENLDQ--F 306

Query: 285 QPNEFVFGSAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSART 344
           +P+     S   AC  L + +  + I+   +K G   +      L D+YAKCG + +AR 
Sbjct: 307 KPDLLTVSSVLRACGHLRDLSLAKYIYNYMLKAGFVLESTVRNILIDVYAKCGDMITARD 366

Query: 345 VFYHIEKPDLVAWNSIIAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVM 404
           VF  +E  D V+WNSII+G+   GD  E++  F  M       + +T L L+   +    
Sbjct: 367 VFNSMECKDTVSWNSIISGYIQSGDLMEAMKLFKMMMIMEEQADHITYLMLISVSTRLAD 426

Query: 405 LNQGMQVHSYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLT 464
           L  G  +HS  ++ G  +D+ V N+L+ MY+KC  + D+L+IF  +G   D V+WNT+++
Sbjct: 427 LKFGKGLHSNGIKSGICIDLSVSNALIDMYAKCGEVGDSLKIFSSMGT-GDTVTWNTVIS 486

Query: 465 ACLQQNQAGEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNL 524
           AC++       L++T  M  S + PD  T    L     +A+  +G ++HC +++ G   
Sbjct: 487 ACVRFGDFATGLQVTTQMRKSEVVPDMATFLVTLPMCASLAAKRLGKEIHCCLLRFGYES 546

Query: 525 DTSVSNALINMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMR 584
           +  + NALI MY+KCG L  + ++F+ +   D+++W+ +I  Y   G GE+A E F  M 
Sbjct: 547 ELQIGNALIEMYSKCGCLENSSRVFERMSRRDVVTWTGMIYAYGMYGEGEKALETFADME 606

Query: 585 GLGVKPNEITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCL 644
             G+ P+ + F+ I+ ACSH G+V+EGL  +  M+  Y I P  EH +C+VDLL+R+  +
Sbjct: 607 KSGIVPDSVVFIAIIYACSHSGLVDEGLACFEKMKTHYKIDPMIEHYACVVDLLSRSQKI 666

Query: 645 DGAEDFIKHMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIH 704
             AE+FI+ MP  PD  +W ++L AC+  G++E  +R +  +++++P +    ++  N +
Sbjct: 667 SKAEEFIQAMPIKPDASIWASVLRACRTSGDMETAERVSRRIIELNPDDPGYSILASNAY 726

Query: 705 ASSGCWKDFAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELM 764
           A+   W   +  R S++  +++K PG SWIE+   VHVF + D   P+   IY  LE L 
Sbjct: 727 AALRKWDKVSLIRKSLKDKHITKNPGYSWIEVGKNVHVFSSGDDSAPQSEAIYKSLEILY 786

Query: 765 LQVLDDGCDP 775
             +  +G  P
Sbjct: 787 SLMAKEGYIP 792

BLAST of Lsi03G020440 vs. ExPASy Swiss-Prot
Match: Q5G1T1 (Pentatricopeptide repeat-containing protein At3g49170, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=EMB2261 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.5e-131
Identity = 248/719 (34.49%), Postives = 410/719 (57.02%), Query Frame = 0

Query: 66  REALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQNH 125
           R A+ A D+  +    P+ S+T++ L+ +C   R    G+ +H  ++ F+ +PD +L N 
Sbjct: 43  RGAVSALDLMARDGIRPMDSVTFSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNS 102

Query: 126 ILNMYGKCGSLKEARNIFDAM---PLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGY 185
           ++++Y K G   +A ++F+ M     ++VVSW++M++ Y + G+E +AI ++V+ L  G 
Sbjct: 103 LISLYSKSGDSAKAEDVFETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGL 162

Query: 186 IPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKS-EFGSHLIAQNALISMYTK-LSQMADA 245
           +P+ + + +V+++CS  D   + R     ++K+  F S +    +LI M+ K  +   +A
Sbjct: 163 VPNDYCYTAVIRACSNSDFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENA 222

Query: 246 KNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSK 305
             VF  +   ++++W  MI    Q+G+  EA+  F +M+    ++ ++F   S FSAC++
Sbjct: 223 YKVFDKMSELNVVTWTLMITRCMQMGFPREAIRFFLDMVLSG-FESDKFTLSSVFSACAE 282

Query: 306 LLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKC---GFLGSARTVFYHIEKPDLVAW 365
           L   + G+Q+H   I+ GL  D+   CSL DMYAKC   G +   R VF  +E   +++W
Sbjct: 283 LENLSLGKQLHSWAIRSGLVDDV--ECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSW 342

Query: 366 NSIIAGF-ASVGDAKESLSFFSRMRHTGLV-PNDVTVLSLLCACSEPVMLNQGMQVHSYI 425
            ++I G+  +   A E+++ FS M   G V PN  T  S   AC        G QV    
Sbjct: 343 TALITGYMKNCNLATEAINLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQA 402

Query: 426 VRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEV 485
            + G   +  V NS++SM+ K   + DA + FE +  K ++VS+NT L    +     + 
Sbjct: 403 FKRGLASNSSVANSVISMFVKSDRMEDAQRAFESLSEK-NLVSYNTFLDGTCRNLNFEQA 462

Query: 486 LRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINM 545
            +L   +    +     T  ++L     + S   G Q+H  ++K GL+ +  V NALI+M
Sbjct: 463 FKLLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISM 522

Query: 546 YTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITF 605
           Y+KCGS+  A ++F+ ++N ++ISW+S+I G+A+ G      E F  M   GVKPNE+T+
Sbjct: 523 YSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTY 582

Query: 606 VGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMP 665
           V IL+ACSH+G+V EG + + +M E++ I P  EH +CMVDLL RAG L  A +FI  MP
Sbjct: 583 VAILSACSHVGLVSEGWRHFNSMYEDHKIKPKMEHYACMVDLLCRAGLLTDAFEFINTMP 642

Query: 666 FDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQ 725
           F  D++VW+T L AC+VH N E+GK AA  +L++DP+  AA + L NI+A +G W++  +
Sbjct: 643 FQADVLVWRTFLGACRVHSNTELGKLAARKILELDPNEPAAYIQLSNIYACAGKWEESTE 702

Query: 726 YRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDP 775
            R  M++ N+ K  G SWIE+ DK+H F   D+ HP   +IY  L+ L+ ++   G  P
Sbjct: 703 MRRKMKERNLVKEGGCSWIEVGDKIHKFYVGDTAHPNAHQIYDELDRLITEIKRCGYVP 757

BLAST of Lsi03G020440 vs. ExPASy Swiss-Prot
Match: Q9SS83 (Pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E88 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 9.3e-131
Identity = 245/712 (34.41%), Postives = 390/712 (54.78%), Query Frame = 0

Query: 61   KKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDM 120
            K+ C   A++ F   +K S    +S T   +++A   + +L+ G  +H   +      ++
Sbjct: 304  KRGCETVAIEYFFNMRKSSVKSTRS-TLGSVLSAIGIVANLDLGLVVHAEAIKLGLASNI 363

Query: 121  ILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRS 180
             + + +++MY KC  ++ A  +F+A+  KN V W +MI GY+H G+    + L++ M  S
Sbjct: 364  YVGSSLVSMYSKCEKMEAAAKVFEALEEKNDVFWNAMIRGYAHNGESHKVMELFMDMKSS 423

Query: 181  GYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADA 240
            GY  D FTF S++ +C+   D  +  Q H+ ++K +   +L   NAL+ MY K   + DA
Sbjct: 424  GYNIDDFTFTSLLSTCAASHDLEMGSQFHSIIIKKKLAKNLFVGNALVDMYAKCGALEDA 483

Query: 241  KNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSK 300
            + +F  +  +D ++W ++I  + Q   E EA   F+ M    +         S   AC+ 
Sbjct: 484  RQIFERMCDRDNVTWNTIIGSYVQDENESEAFDLFKRMNLCGIVSDGA-CLASTLKACTH 543

Query: 301  LLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSI 360
            +     G+Q+H L +K GL  DL  G SL DMY+KCG +  AR VF  + +  +V+ N++
Sbjct: 544  VHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMYSKCGIIKDARKVFSSLPEWSVVSMNAL 603

Query: 361  IAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGF 420
            IAG+ S  + +E++  F  M   G+ P+++T  +++ AC +P  L  G Q H  I + GF
Sbjct: 604  IAGY-SQNNLEEAVVLFQEMLTRGVNPSEITFATIVEACHKPESLTLGTQFHGQITKRGF 663

Query: 421  DLDIPVCN-SLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLT 480
              +      SLL MY     + +A  +F  + +   IV W  M++   Q     E L+  
Sbjct: 664  SSEGEYLGISLLGMYMNSRGMTEACALFSELSSPKSIVLWTGMMSGHSQNGFYEEALKFY 723

Query: 481  KLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKC 540
            K M    + PD  T   VL     ++S   G  +H  I     +LD   SN LI+MY KC
Sbjct: 724  KEMRHDGVLPDQATFVTVLRVCSVLSSLREGRAIHSLIFHLAHDLDELTSNTLIDMYAKC 783

Query: 541  GSLGCARKMFDSI-DNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGI 600
            G +  + ++FD +    +++SW+SLI GYA+ G  E+A ++F +MR   + P+EITF+G+
Sbjct: 784  GDMKGSSQVFDEMRRRSNVVSWNSLINGYAKNGYAEDALKIFDSMRQSHIMPDEITFLGV 843

Query: 601  LTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDP 660
            LTACSH G V +G K++  M  +Y I    +H +CMVDLL R G L  A+DFI+     P
Sbjct: 844  LTACSHAGKVSDGRKIFEMMIGQYGIEARVDHVACMVDLLGRWGYLQEADDFIEAQNLKP 903

Query: 661  DIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRS 720
            D  +W +LL AC++HG+   G+ +AE +++++P NS+A V+L NI+AS GCW+     R 
Sbjct: 904  DARLWSSLLGACRIHGDDIRGEISAEKLIELEPQNSSAYVLLSNIYASQGCWEKANALRK 963

Query: 721  SMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDD 771
             MR   V KVPG SWI+++ + H+F A D  H E GKI   LE+L   + DD
Sbjct: 964  VMRDRGVKKVPGYSWIDVEQRTHIFAAGDKSHSEIGKIEMFLEDLYDLMKDD 1012

BLAST of Lsi03G020440 vs. ExPASy Swiss-Prot
Match: Q9SVA5 (Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E52 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.0e-129
Identity = 249/720 (34.58%), Postives = 408/720 (56.67%), Query Frame = 0

Query: 56  LISLCKKN-CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGR----KIHRH 115
           ++S C  +  + E+L  F  F +           +  I ACS L     GR    ++   
Sbjct: 116 MVSACNHHGIYEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDG--RGRWMVFQLQSF 175

Query: 116 MLTFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNA 175
           ++   +  D+ +   +++ Y K G++  AR +FDA+P K+ V+WT+MISG    G+   +
Sbjct: 176 LVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVS 235

Query: 176 IALYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISM 235
           + L+ Q++    +PD +   +V+ +CS L      +Q+HAH+L+          N LI  
Sbjct: 236 LQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDS 295

Query: 236 YTKLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFV 295
           Y K  ++  A  +F+ +  K++ISW ++++G+ Q     EA+  F  M S+   +P+ + 
Sbjct: 296 YVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSM-SKFGLKPDMYA 355

Query: 296 FGSAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIE 355
             S  ++C+ L    +G Q+H   IK  LG+D +   SL DMYAKC  L  AR VF    
Sbjct: 356 CSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFA 415

Query: 356 KPDLVAWNSIIAGFASVG---DAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQ 415
             D+V +N++I G++ +G   +  E+L+ F  MR   + P+ +T +SLL A +    L  
Sbjct: 416 AADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGL 475

Query: 416 GMQVHSYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACL 475
             Q+H  + + G +LDI   ++L+ +YS C  L D+  +F+ +  K D+V WN+M    +
Sbjct: 476 SKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDEMKVK-DLVIWNSMFAGYV 535

Query: 476 QQNQAGEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTS 535
           QQ++  E L L   +  S  +PD  T  N++ ++G +AS ++G + HC ++K GL  +  
Sbjct: 536 QQSENEEALNLFLELQLSRERPDEFTFANMVTAAGNLASVQLGQEFHCQLLKRGLECNPY 595

Query: 536 VSNALINMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLG 595
           ++NAL++MY KCGS   A K FDS  + D++ W+S+I  YA  G G++A ++   M   G
Sbjct: 596 ITNALLDMYAKCGSPEDAHKAFDSAASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEG 655

Query: 596 VKPNEITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGA 655
           ++PN ITFVG+L+ACSH G+VE+GLK +  M   + I P  EH  CMV LL RAG L+ A
Sbjct: 656 IEPNYITFVGVLSACSHAGLVEDGLKQFELML-RFGIEPETEHYVCMVSLLGRAGRLNKA 715

Query: 656 EDFIKHMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASS 715
            + I+ MP  P  +VW++LL+ C   GN+E+ + AAE  +  DP +S +  ML NI+AS 
Sbjct: 716 RELIEKMPTKPAAIVWRSLLSGCAKAGNVELAEHAAEMAILSDPKDSGSFTMLSNIYASK 775

Query: 716 GCWKDFAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQV 768
           G W +  + R  M+   V K PG+SWI I  +VH+FL++D  H +  +IY +L++L++Q+
Sbjct: 776 GMWTEAKKVRERMKVEGVVKEPGRSWIGINKEVHIFLSKDKSHCKANQIYEVLDDLLVQI 830

BLAST of Lsi03G020440 vs. ExPASy TrEMBL
Match: A0A0A0LDF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736910 PE=4 SV=1)

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 707/774 (91.34%), Postives = 742/774 (95.87%), Query Frame = 0

Query: 4   SDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKN 63
           SDAIVFFNPFSPT+S KNFKPQLHLALSHIRLNTQLAFSP P+T HY HDDK+ISLCKKN
Sbjct: 17  SDAIVFFNPFSPTSSIKNFKPQLHLALSHIRLNTQLAFSPCPLTVHYPHDDKIISLCKKN 76

Query: 64  CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQ 123
            HREAL+AFDIFQKCS+SPLKS+TYTHLINACSSLRSLEHGRKIHRHMLT NYQPDMILQ
Sbjct: 77  LHREALKAFDIFQKCSSSPLKSVTYTHLINACSSLRSLEHGRKIHRHMLTCNYQPDMILQ 136

Query: 124 NHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYI 183
           NHIL+MYGKCGSLKEARN+FD+MPLKNVVSWTSMISGYS YG+EDNAI LYVQMLRSG+I
Sbjct: 137 NHILSMYGKCGSLKEARNMFDSMPLKNVVSWTSMISGYSRYGEEDNAITLYVQMLRSGHI 196

Query: 184 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNV 243
           PDHFTFGS+VKSCSGLDDF LARQLHAHVLKSEFG+ LIAQNALISMYTK SQMADA NV
Sbjct: 197 PDHFTFGSIVKSCSGLDDFKLARQLHAHVLKSEFGADLIAQNALISMYTKFSQMADAINV 256

Query: 244 FSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLE 303
           FS IIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQ VYQPNEFVFGSAFSACSKLLE
Sbjct: 257 FSRIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQSVYQPNEFVFGSAFSACSKLLE 316

Query: 304 PNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAG 363
           P+ GRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFL SARTVFYHIEKPDLVAWN+IIAG
Sbjct: 317 PDCGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLESARTVFYHIEKPDLVAWNAIIAG 376

Query: 364 FASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLD 423
           FASV +AKES SFFS+MRHTGLVPNDVTVLSLLCACSEPVMLN G+QVHSYIV+MGF+LD
Sbjct: 377 FASVSNAKESSSFFSQMRHTGLVPNDVTVLSLLCACSEPVMLNHGIQVHSYIVKMGFNLD 436

Query: 424 IPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLML 483
           IPVCNSLLSMYSKCSNLNDALQ+FE IGNKADIVSWNT+LTACLQQNQAGEVLRLTKLM 
Sbjct: 437 IPVCNSLLSMYSKCSNLNDALQVFEDIGNKADIVSWNTLLTACLQQNQAGEVLRLTKLMF 496

Query: 484 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLG 543
           AS IKPDHVTLTNVLVSSGQIASYEVGSQ+HCFIMKSGLNLD SVSNALINMYTKCGSL 
Sbjct: 497 ASRIKPDHVTLTNVLVSSGQIASYEVGSQIHCFIMKSGLNLDISVSNALINMYTKCGSLE 556

Query: 544 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACS 603
           CARKMFDSI NPDIISWSSLIVGYAQAGCG+EAFELFRTMRGLGVKPNEITFVGILTACS
Sbjct: 557 CARKMFDSIGNPDIISWSSLIVGYAQAGCGKEAFELFRTMRGLGVKPNEITFVGILTACS 616

Query: 604 HIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVW 663
           HIGMVEEGLKLYRTMQE+Y ISPTKEHCSCMVDLLARAGCLD AEDFIK MPF PD+VVW
Sbjct: 617 HIGMVEEGLKLYRTMQEDYRISPTKEHCSCMVDLLARAGCLDVAEDFIKQMPFVPDVVVW 676

Query: 664 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQM 723
           KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAA+VMLCNIHASSG WKDFA+ RSSMR+M
Sbjct: 677 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAAVVMLCNIHASSGHWKDFARLRSSMRRM 736

Query: 724 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +V KVPGQSWIEIKDKVHVFLAED+LHPERGKIYTMLEELMLQ+LDDGCDP+++
Sbjct: 737 DVGKVPGQSWIEIKDKVHVFLAEDNLHPERGKIYTMLEELMLQILDDGCDPLQM 790

BLAST of Lsi03G020440 vs. ExPASy TrEMBL
Match: A0A5A7TPL0 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002390 PE=4 SV=1)

HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 705/774 (91.09%), Postives = 736/774 (95.09%), Query Frame = 0

Query: 4   SDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKN 63
           SDAIVFFNPFSP+TSTKNFKPQLHLA SHIRL TQLAFSP PVT HY HDDK+ISLCKKN
Sbjct: 17  SDAIVFFNPFSPSTSTKNFKPQLHLAHSHIRLGTQLAFSPCPVTVHYPHDDKIISLCKKN 76

Query: 64  CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQ 123
            HREALQAFDIF+KCS+SPLKSITYTHLINACSSLRSLEHGRKIHRHMLT NYQPDMILQ
Sbjct: 77  LHREALQAFDIFRKCSSSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTCNYQPDMILQ 136

Query: 124 NHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYI 183
           NHIL+MYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYS YGQEDNAI LY+QMLRSGYI
Sbjct: 137 NHILSMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSRYGQEDNAITLYIQMLRSGYI 196

Query: 184 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNV 243
           PDHFTFGS+VKSCSGLDDFMLARQLHAHVLK EFG HLIAQNALISMYTK SQMADA NV
Sbjct: 197 PDHFTFGSIVKSCSGLDDFMLARQLHAHVLKFEFGGHLIAQNALISMYTKFSQMADAINV 256

Query: 244 FSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLE 303
           FS IIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQ VYQPNEFVFGSAFSACSKLLE
Sbjct: 257 FSRIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQSVYQPNEFVFGSAFSACSKLLE 316

Query: 304 PNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAG 363
           P+ GRQIHGLCIK GLGSD+FAGCSLCDMYAKCGFL SARTVFYHIEKPDLVAWN+IIAG
Sbjct: 317 PDCGRQIHGLCIKLGLGSDIFAGCSLCDMYAKCGFLESARTVFYHIEKPDLVAWNAIIAG 376

Query: 364 FASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLD 423
           FASV +AKESLSFFS+MRH G+VPNDVTVLSLLCACSEPVMLN GMQVHSYIV+MGF+LD
Sbjct: 377 FASVSNAKESLSFFSQMRHRGVVPNDVTVLSLLCACSEPVMLNNGMQVHSYIVKMGFNLD 436

Query: 424 IPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLML 483
           IPVCNSLLSMYSKCSNLNDALQ+FEGIGNKADIVSWNT+LTACLQQNQAGEVLRLTKLM 
Sbjct: 437 IPVCNSLLSMYSKCSNLNDALQVFEGIGNKADIVSWNTLLTACLQQNQAGEVLRLTKLMF 496

Query: 484 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLG 543
           AS I PDHVTLTNVLVSSGQIASYEVGSQ+HCFIMKSGLNLDTSVSNALINMYTKCGSL 
Sbjct: 497 ASRIMPDHVTLTNVLVSSGQIASYEVGSQIHCFIMKSGLNLDTSVSNALINMYTKCGSLE 556

Query: 544 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACS 603
           CARKMFDSI NPDIISWSSLIVGYAQAGCG+EAFELFRTMRGLGVKPNEITF+GILTACS
Sbjct: 557 CARKMFDSIGNPDIISWSSLIVGYAQAGCGKEAFELFRTMRGLGVKPNEITFLGILTACS 616

Query: 604 HIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVW 663
           HIGMVEEGLKLYRTMQE+  ISPTKEHCSC+VDLLARAGCLD AE+FIK MPF PD+VVW
Sbjct: 617 HIGMVEEGLKLYRTMQEDCRISPTKEHCSCIVDLLARAGCLDVAEEFIKQMPFVPDVVVW 676

Query: 664 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQM 723
           KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAA+VMLCNIHASSG WKD AQ R SMR+M
Sbjct: 677 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAAVVMLCNIHASSGHWKDLAQLRISMRRM 736

Query: 724 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +V KVPGQSWIEIKDKVHVFLAED+LHPERGKIYTMLEELMLQ LDD CDP+++
Sbjct: 737 DVGKVPGQSWIEIKDKVHVFLAEDNLHPERGKIYTMLEELMLQTLDDSCDPLQM 790

BLAST of Lsi03G020440 vs. ExPASy TrEMBL
Match: A0A1S3B684 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486463 PE=4 SV=1)

HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 705/774 (91.09%), Postives = 736/774 (95.09%), Query Frame = 0

Query: 4   SDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKN 63
           SDAIVFFNPFSP+TSTKNFKPQLHLA SHIRL TQLAFSP PVT HY HDDK+ISLCKKN
Sbjct: 17  SDAIVFFNPFSPSTSTKNFKPQLHLAHSHIRLGTQLAFSPCPVTVHYPHDDKIISLCKKN 76

Query: 64  CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQ 123
            HREALQAFDIF+KCS+SPLKSITYTHLINACSSLRSLEHGRKIHRHMLT NYQPDMILQ
Sbjct: 77  LHREALQAFDIFRKCSSSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTCNYQPDMILQ 136

Query: 124 NHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYI 183
           NHIL+MYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYS YGQEDNAI LY+QMLRSGYI
Sbjct: 137 NHILSMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSRYGQEDNAITLYIQMLRSGYI 196

Query: 184 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNV 243
           PDHFTFGS+VKSCSGLDDFMLARQLHAHVLK EFG HLIAQNALISMYTK SQMADA NV
Sbjct: 197 PDHFTFGSIVKSCSGLDDFMLARQLHAHVLKFEFGGHLIAQNALISMYTKFSQMADAINV 256

Query: 244 FSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLE 303
           FS IIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQ VYQPNEFVFGSAFSACSKLLE
Sbjct: 257 FSRIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQSVYQPNEFVFGSAFSACSKLLE 316

Query: 304 PNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAG 363
           P+ GRQIHGLCIK GLGSD+FAGCSLCDMYAKCGFL SARTVFYHIEKPDLVAWN+IIAG
Sbjct: 317 PDCGRQIHGLCIKLGLGSDIFAGCSLCDMYAKCGFLESARTVFYHIEKPDLVAWNAIIAG 376

Query: 364 FASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLD 423
           FASV +AKESLSFFS+MRH G+VPNDVTVLSLLCACSEPVMLN GMQVHSYIV+MGF+LD
Sbjct: 377 FASVSNAKESLSFFSQMRHRGVVPNDVTVLSLLCACSEPVMLNNGMQVHSYIVKMGFNLD 436

Query: 424 IPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLML 483
           IPVCNSLLSMYSKCSNLNDALQ+FEGIGNKADIVSWNT+LTACLQQNQAGEVLRLTKLM 
Sbjct: 437 IPVCNSLLSMYSKCSNLNDALQVFEGIGNKADIVSWNTLLTACLQQNQAGEVLRLTKLMF 496

Query: 484 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLG 543
           AS I PDHVTLTNVLVSSGQIASYEVGSQ+HCFIMKSGLNLDTSVSNALINMYTKCGSL 
Sbjct: 497 ASRIMPDHVTLTNVLVSSGQIASYEVGSQIHCFIMKSGLNLDTSVSNALINMYTKCGSLE 556

Query: 544 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACS 603
           CARKMFDSI NPDIISWSSLIVGYAQAGCG+EAFELFRTMRGLGVKPNEITF+GILTACS
Sbjct: 557 CARKMFDSIGNPDIISWSSLIVGYAQAGCGKEAFELFRTMRGLGVKPNEITFLGILTACS 616

Query: 604 HIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVW 663
           HIGMVEEGLKLYRTMQE+  ISPTKEHCSC+VDLLARAGCLD AE+FIK MPF PD+VVW
Sbjct: 617 HIGMVEEGLKLYRTMQEDCRISPTKEHCSCIVDLLARAGCLDVAEEFIKQMPFVPDVVVW 676

Query: 664 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQM 723
           KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAA+VMLCNIHASSG WKD AQ R SMR+M
Sbjct: 677 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAAVVMLCNIHASSGHWKDLAQLRISMRRM 736

Query: 724 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +V KVPGQSWIEIKDKVHVFLAED+LHPERGKIYTMLEELMLQ LDD CDP+++
Sbjct: 737 DVGKVPGQSWIEIKDKVHVFLAEDNLHPERGKIYTMLEELMLQTLDDSCDPLQM 790

BLAST of Lsi03G020440 vs. ExPASy TrEMBL
Match: A0A6J1F1S7 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111441412 PE=4 SV=1)

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 674/776 (86.86%), Postives = 720/776 (92.78%), Query Frame = 0

Query: 3   TSDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAF-SPFPVTDHYSHDDKLISLCK 62
           +S+AIVFFNP SP TS KNFKPQL LALSHIR ++ LA   P PVT+H S+DD +ISLCK
Sbjct: 44  SSEAIVFFNPCSPATSIKNFKPQLRLALSHIRFSSLLASPPPSPVTEH-SYDDNIISLCK 103

Query: 63  KNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMI 122
           K  HREALQAFDIFQKCS+SPL SITYTHLI+ACSSLRSLEHGRKIH HM TFNYQPD+I
Sbjct: 104 KKLHREALQAFDIFQKCSSSPLNSITYTHLIHACSSLRSLEHGRKIHCHMSTFNYQPDLI 163

Query: 123 LQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSG 182
           LQNHILNMYGKCGSLKEARNIFDAMPLKN VSWTSMISGYSHYGQ+DNAI LYVQMLRSG
Sbjct: 164 LQNHILNMYGKCGSLKEARNIFDAMPLKNAVSWTSMISGYSHYGQDDNAITLYVQMLRSG 223

Query: 183 YIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAK 242
           +IPDHFTFGSVVKSCSGLDD MLARQLHAHVLKSEFG + IAQNALISMYTK SQ+ADA 
Sbjct: 224 HIPDHFTFGSVVKSCSGLDDLMLARQLHAHVLKSEFGGNPIAQNALISMYTKFSQIADAT 283

Query: 243 NVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKL 302
           NVFSHIIIKDLISWGSMIAGFSQLGYE+EALCHFREMLSQ +YQPNEFVFGSAFSACS L
Sbjct: 284 NVFSHIIIKDLISWGSMIAGFSQLGYEIEALCHFREMLSQAIYQPNEFVFGSAFSACSSL 343

Query: 303 LEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSII 362
            EPN GRQIHGLCIKFGLGSD FAGCSLCDMYAKCGFLGSARTVF  IEKPDLVAWN+II
Sbjct: 344 SEPNCGRQIHGLCIKFGLGSDRFAGCSLCDMYAKCGFLGSARTVFCQIEKPDLVAWNAII 403

Query: 363 AGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFD 422
           AGFASVGDAKESLSFFS+MRHTGL  NDVTVLSLLCACS+P+MLNQGMQVHSYIV+ GFD
Sbjct: 404 AGFASVGDAKESLSFFSQMRHTGLASNDVTVLSLLCACSDPMMLNQGMQVHSYIVKTGFD 463

Query: 423 LDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKL 482
           L++PVCN LLSMYSKCS LND+L+IFE IGNKADIVSWNTMLTAC  QNQAGEVLRL KL
Sbjct: 464 LEVPVCNGLLSMYSKCSVLNDSLKIFEDIGNKADIVSWNTMLTACRLQNQAGEVLRLMKL 523

Query: 483 MLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGS 542
           MLASHIK D VTLTNVLVSSGQIASYEVGSQVHCFIMKSG NLDTSVSNALINMY KCGS
Sbjct: 524 MLASHIKSDRVTLTNVLVSSGQIASYEVGSQVHCFIMKSGQNLDTSVSNALINMYMKCGS 583

Query: 543 LGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTA 602
           LGCARKMFDSI++PD+ISWSSLIVGYAQAGCGEEAFELFRTMRGLG++PNEITF+GILTA
Sbjct: 584 LGCARKMFDSINDPDVISWSSLIVGYAQAGCGEEAFELFRTMRGLGIRPNEITFIGILTA 643

Query: 603 CSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIV 662
           C HIGMVEEGL+LYRTMQE+  ISPTKEHCSC+VDLLARAGCLD AEDFIK MPF+PDIV
Sbjct: 644 CCHIGMVEEGLRLYRTMQEQDGISPTKEHCSCIVDLLARAGCLDAAEDFIKKMPFEPDIV 703

Query: 663 VWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMR 722
           VW TLLAACK+HGNLEVGKRAAENVLK DPSNSAALVMLCNIHASSG WKDFA+ R SMR
Sbjct: 704 VWMTLLAACKLHGNLEVGKRAAENVLKNDPSNSAALVMLCNIHASSGHWKDFARLRRSMR 763

Query: 723 QMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +M+VSKVPGQSWIEIKD+VHVF AEDSLHPERG+IYTMLEELM+Q+LDD CDP+++
Sbjct: 764 RMDVSKVPGQSWIEIKDRVHVFFAEDSLHPERGRIYTMLEELMVQILDDSCDPLQM 818

BLAST of Lsi03G020440 vs. ExPASy TrEMBL
Match: A0A6J1J911 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111482386 PE=4 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 670/775 (86.45%), Postives = 717/775 (92.52%), Query Frame = 0

Query: 3   TSDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKK 62
           +S+AIVFFNP SP TS KNFKPQLHLALSHIR ++ LA  P PVT+H SHDD +ISLCKK
Sbjct: 44  SSEAIVFFNPCSPATSIKNFKPQLHLALSHIRFSSLLASPPSPVTEH-SHDDNIISLCKK 103

Query: 63  NCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMIL 122
             HREALQAFDIF KCS+SPL SITYTHLI+ACSSLR LEHGRKIH HM TFNYQPD+IL
Sbjct: 104 KLHREALQAFDIFHKCSSSPLNSITYTHLIHACSSLRFLEHGRKIHCHMSTFNYQPDLIL 163

Query: 123 QNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGY 182
           QNHILNMYGKCGSLKEARNIFDAMPLKN VSWTSMISGYSHYG++DNAI LYVQMLRSG+
Sbjct: 164 QNHILNMYGKCGSLKEARNIFDAMPLKNAVSWTSMISGYSHYGEDDNAITLYVQMLRSGH 223

Query: 183 IPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKN 242
           IPDHFTFGSVVKSCSGLDD MLARQLHAHVLKSEFG + IAQNALISMYTK SQ+ADA N
Sbjct: 224 IPDHFTFGSVVKSCSGLDDLMLARQLHAHVLKSEFGGNPIAQNALISMYTKFSQIADATN 283

Query: 243 VFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLL 302
           VFSHII KDLISWGSMIAGFSQLG E+EALCHFREMLSQP+YQPNEFVFGSAFSACS L 
Sbjct: 284 VFSHIITKDLISWGSMIAGFSQLGCEIEALCHFREMLSQPIYQPNEFVFGSAFSACSNLS 343

Query: 303 EPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIA 362
           EPN GRQIHGLCIKFGLGSD FAGCSLCDMYAKCGFLGSARTVF  IEKPDLVAWN+IIA
Sbjct: 344 EPNCGRQIHGLCIKFGLGSDRFAGCSLCDMYAKCGFLGSARTVFCQIEKPDLVAWNAIIA 403

Query: 363 GFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDL 422
           GFASVGDAKESLSFFS+MRHTGL  NDVTVLSLLCACSEP+MLNQGMQVHSYIV+ GFDL
Sbjct: 404 GFASVGDAKESLSFFSQMRHTGLASNDVTVLSLLCACSEPMMLNQGMQVHSYIVKTGFDL 463

Query: 423 DIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLM 482
           ++ VCN LLSMYSKCS+LND+L+IFE IGNKAD+VSWNTMLTAC  +NQAGEVLRL KLM
Sbjct: 464 EVLVCNGLLSMYSKCSDLNDSLKIFEDIGNKADVVSWNTMLTACRLRNQAGEVLRLMKLM 523

Query: 483 LASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSL 542
           LASHIK D VTLTNVLVSSG IASYEVGSQVHCFIMKSG NLDTSVSNALINMY KCGSL
Sbjct: 524 LASHIKSDRVTLTNVLVSSGLIASYEVGSQVHCFIMKSGQNLDTSVSNALINMYMKCGSL 583

Query: 543 GCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTAC 602
           GCARKMFDSID+PD+ISWSSLIVGYAQAGCGEEAFELFRTMRGLG++PNEITF+GILTAC
Sbjct: 584 GCARKMFDSIDDPDVISWSSLIVGYAQAGCGEEAFELFRTMRGLGIRPNEITFLGILTAC 643

Query: 603 SHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVV 662
            H+GMVEEGL+LYRTMQE+ DISPTKEHCSC+VDLLARAGCLD AEDFIK MPF+PDIVV
Sbjct: 644 CHVGMVEEGLRLYRTMQEQDDISPTKEHCSCIVDLLARAGCLDAAEDFIKKMPFEPDIVV 703

Query: 663 WKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQ 722
           W TLLAACKVHGNLEVGKRAAENVL+ DPSNSAALVMLCNIHASSG WKDFA+ R SMR+
Sbjct: 704 WMTLLAACKVHGNLEVGKRAAENVLRNDPSNSAALVMLCNIHASSGHWKDFARLRRSMRR 763

Query: 723 MNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           M+VSKVPGQSWIEIKD+VHVF AEDSLHPERG+IY MLEELMLQ+LDD CDP+++
Sbjct: 764 MDVSKVPGQSWIEIKDRVHVFFAEDSLHPERGRIYIMLEELMLQLLDDSCDPLQM 817

BLAST of Lsi03G020440 vs. NCBI nr
Match: XP_038903429.1 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1494.6 bits (3868), Expect = 0.0e+00
Identity = 725/776 (93.43%), Postives = 749/776 (96.52%), Query Frame = 0

Query: 4   SDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKN 63
           SDA VFF PFSPT S KNFK +LHLALSHIRLNT LAFSP PVT+HYSHDDK+ISLCKKN
Sbjct: 17  SDASVFFTPFSPTASIKNFKSRLHLALSHIRLNTHLAFSPCPVTEHYSHDDKIISLCKKN 76

Query: 64  CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQ 123
            HREALQAFDIFQKCS+SPLKSITYTHLINACSSLRSLEHGR+IHRHMLTFN QPDMILQ
Sbjct: 77  LHREALQAFDIFQKCSSSPLKSITYTHLINACSSLRSLEHGREIHRHMLTFNCQPDMILQ 136

Query: 124 NHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYI 183
           NHILNMYGKCGSLKEARNIF+AMPLKNVVSWTSMISGYSHYGQEDNAI +YVQMLRSGYI
Sbjct: 137 NHILNMYGKCGSLKEARNIFNAMPLKNVVSWTSMISGYSHYGQEDNAITMYVQMLRSGYI 196

Query: 184 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNV 243
           PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFG HLIAQNALISMYTK S++ADA NV
Sbjct: 197 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGGHLIAQNALISMYTKFSRIADATNV 256

Query: 244 FSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLE 303
           FSHI+IKDLISWGSMIAGFSQLGYELEALCHFREM+SQPVYQPNEFVFGSAFSACSKLLE
Sbjct: 257 FSHIVIKDLISWGSMIAGFSQLGYELEALCHFREMVSQPVYQPNEFVFGSAFSACSKLLE 316

Query: 304 PNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAG 363
           PN GRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWN+IIAG
Sbjct: 317 PNCGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNAIIAG 376

Query: 364 FASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLD 423
           FASVGDAKESLSFFS+MRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSY+V+MGFDLD
Sbjct: 377 FASVGDAKESLSFFSQMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYVVKMGFDLD 436

Query: 424 IPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLML 483
           IPV N+LL MYSKCSNL DAL IFEGIG KADIVSWNT+LTACLQQNQAGEVLRLTKLML
Sbjct: 437 IPVSNTLLGMYSKCSNLTDALHIFEGIGTKADIVSWNTLLTACLQQNQAGEVLRLTKLML 496

Query: 484 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLG 543
           ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALIN YTKCGSLG
Sbjct: 497 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINTYTKCGSLG 556

Query: 544 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACS 603
           CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAF LFRTMR LGVKPNEITFVGIL ACS
Sbjct: 557 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFVLFRTMRSLGVKPNEITFVGILIACS 616

Query: 604 HIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVW 663
           HIGMVEEGLKLY+TMQ+EY ISPTKEHCSCMVDLLARAGCLDGAE+FIK MPF+PDIVVW
Sbjct: 617 HIGMVEEGLKLYKTMQKEYGISPTKEHCSCMVDLLARAGCLDGAENFIKQMPFNPDIVVW 676

Query: 664 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQM 723
           KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSG WKDFAQ RSSMRQM
Sbjct: 677 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGHWKDFAQCRSSMRQM 736

Query: 724 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKVTK 780
           NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYT+LEELMLQ+LDDGCDPI+VTK
Sbjct: 737 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTILEELMLQILDDGCDPIQVTK 792

BLAST of Lsi03G020440 vs. NCBI nr
Match: XP_004137966.1 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucumis sativus])

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 707/774 (91.34%), Postives = 742/774 (95.87%), Query Frame = 0

Query: 4   SDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKN 63
           SDAIVFFNPFSPT+S KNFKPQLHLALSHIRLNTQLAFSP P+T HY HDDK+ISLCKKN
Sbjct: 17  SDAIVFFNPFSPTSSIKNFKPQLHLALSHIRLNTQLAFSPCPLTVHYPHDDKIISLCKKN 76

Query: 64  CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQ 123
            HREAL+AFDIFQKCS+SPLKS+TYTHLINACSSLRSLEHGRKIHRHMLT NYQPDMILQ
Sbjct: 77  LHREALKAFDIFQKCSSSPLKSVTYTHLINACSSLRSLEHGRKIHRHMLTCNYQPDMILQ 136

Query: 124 NHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYI 183
           NHIL+MYGKCGSLKEARN+FD+MPLKNVVSWTSMISGYS YG+EDNAI LYVQMLRSG+I
Sbjct: 137 NHILSMYGKCGSLKEARNMFDSMPLKNVVSWTSMISGYSRYGEEDNAITLYVQMLRSGHI 196

Query: 184 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNV 243
           PDHFTFGS+VKSCSGLDDF LARQLHAHVLKSEFG+ LIAQNALISMYTK SQMADA NV
Sbjct: 197 PDHFTFGSIVKSCSGLDDFKLARQLHAHVLKSEFGADLIAQNALISMYTKFSQMADAINV 256

Query: 244 FSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLE 303
           FS IIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQ VYQPNEFVFGSAFSACSKLLE
Sbjct: 257 FSRIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQSVYQPNEFVFGSAFSACSKLLE 316

Query: 304 PNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAG 363
           P+ GRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFL SARTVFYHIEKPDLVAWN+IIAG
Sbjct: 317 PDCGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLESARTVFYHIEKPDLVAWNAIIAG 376

Query: 364 FASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLD 423
           FASV +AKES SFFS+MRHTGLVPNDVTVLSLLCACSEPVMLN G+QVHSYIV+MGF+LD
Sbjct: 377 FASVSNAKESSSFFSQMRHTGLVPNDVTVLSLLCACSEPVMLNHGIQVHSYIVKMGFNLD 436

Query: 424 IPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLML 483
           IPVCNSLLSMYSKCSNLNDALQ+FE IGNKADIVSWNT+LTACLQQNQAGEVLRLTKLM 
Sbjct: 437 IPVCNSLLSMYSKCSNLNDALQVFEDIGNKADIVSWNTLLTACLQQNQAGEVLRLTKLMF 496

Query: 484 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLG 543
           AS IKPDHVTLTNVLVSSGQIASYEVGSQ+HCFIMKSGLNLD SVSNALINMYTKCGSL 
Sbjct: 497 ASRIKPDHVTLTNVLVSSGQIASYEVGSQIHCFIMKSGLNLDISVSNALINMYTKCGSLE 556

Query: 544 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACS 603
           CARKMFDSI NPDIISWSSLIVGYAQAGCG+EAFELFRTMRGLGVKPNEITFVGILTACS
Sbjct: 557 CARKMFDSIGNPDIISWSSLIVGYAQAGCGKEAFELFRTMRGLGVKPNEITFVGILTACS 616

Query: 604 HIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVW 663
           HIGMVEEGLKLYRTMQE+Y ISPTKEHCSCMVDLLARAGCLD AEDFIK MPF PD+VVW
Sbjct: 617 HIGMVEEGLKLYRTMQEDYRISPTKEHCSCMVDLLARAGCLDVAEDFIKQMPFVPDVVVW 676

Query: 664 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQM 723
           KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAA+VMLCNIHASSG WKDFA+ RSSMR+M
Sbjct: 677 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAAVVMLCNIHASSGHWKDFARLRSSMRRM 736

Query: 724 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +V KVPGQSWIEIKDKVHVFLAED+LHPERGKIYTMLEELMLQ+LDDGCDP+++
Sbjct: 737 DVGKVPGQSWIEIKDKVHVFLAEDNLHPERGKIYTMLEELMLQILDDGCDPLQM 790

BLAST of Lsi03G020440 vs. NCBI nr
Match: XP_008442662.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucumis melo] >KAA0044026.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK25114.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 705/774 (91.09%), Postives = 736/774 (95.09%), Query Frame = 0

Query: 4   SDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKKN 63
           SDAIVFFNPFSP+TSTKNFKPQLHLA SHIRL TQLAFSP PVT HY HDDK+ISLCKKN
Sbjct: 17  SDAIVFFNPFSPSTSTKNFKPQLHLAHSHIRLGTQLAFSPCPVTVHYPHDDKIISLCKKN 76

Query: 64  CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQ 123
            HREALQAFDIF+KCS+SPLKSITYTHLINACSSLRSLEHGRKIHRHMLT NYQPDMILQ
Sbjct: 77  LHREALQAFDIFRKCSSSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTCNYQPDMILQ 136

Query: 124 NHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGYI 183
           NHIL+MYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYS YGQEDNAI LY+QMLRSGYI
Sbjct: 137 NHILSMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSRYGQEDNAITLYIQMLRSGYI 196

Query: 184 PDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKNV 243
           PDHFTFGS+VKSCSGLDDFMLARQLHAHVLK EFG HLIAQNALISMYTK SQMADA NV
Sbjct: 197 PDHFTFGSIVKSCSGLDDFMLARQLHAHVLKFEFGGHLIAQNALISMYTKFSQMADAINV 256

Query: 244 FSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLLE 303
           FS IIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQ VYQPNEFVFGSAFSACSKLLE
Sbjct: 257 FSRIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQSVYQPNEFVFGSAFSACSKLLE 316

Query: 304 PNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIAG 363
           P+ GRQIHGLCIK GLGSD+FAGCSLCDMYAKCGFL SARTVFYHIEKPDLVAWN+IIAG
Sbjct: 317 PDCGRQIHGLCIKLGLGSDIFAGCSLCDMYAKCGFLESARTVFYHIEKPDLVAWNAIIAG 376

Query: 364 FASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDLD 423
           FASV +AKESLSFFS+MRH G+VPNDVTVLSLLCACSEPVMLN GMQVHSYIV+MGF+LD
Sbjct: 377 FASVSNAKESLSFFSQMRHRGVVPNDVTVLSLLCACSEPVMLNNGMQVHSYIVKMGFNLD 436

Query: 424 IPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLML 483
           IPVCNSLLSMYSKCSNLNDALQ+FEGIGNKADIVSWNT+LTACLQQNQAGEVLRLTKLM 
Sbjct: 437 IPVCNSLLSMYSKCSNLNDALQVFEGIGNKADIVSWNTLLTACLQQNQAGEVLRLTKLMF 496

Query: 484 ASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSLG 543
           AS I PDHVTLTNVLVSSGQIASYEVGSQ+HCFIMKSGLNLDTSVSNALINMYTKCGSL 
Sbjct: 497 ASRIMPDHVTLTNVLVSSGQIASYEVGSQIHCFIMKSGLNLDTSVSNALINMYTKCGSLE 556

Query: 544 CARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTACS 603
           CARKMFDSI NPDIISWSSLIVGYAQAGCG+EAFELFRTMRGLGVKPNEITF+GILTACS
Sbjct: 557 CARKMFDSIGNPDIISWSSLIVGYAQAGCGKEAFELFRTMRGLGVKPNEITFLGILTACS 616

Query: 604 HIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVVW 663
           HIGMVEEGLKLYRTMQE+  ISPTKEHCSC+VDLLARAGCLD AE+FIK MPF PD+VVW
Sbjct: 617 HIGMVEEGLKLYRTMQEDCRISPTKEHCSCIVDLLARAGCLDVAEEFIKQMPFVPDVVVW 676

Query: 664 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQM 723
           KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAA+VMLCNIHASSG WKD AQ R SMR+M
Sbjct: 677 KTLLAACKVHGNLEVGKRAAENVLKIDPSNSAAVVMLCNIHASSGHWKDLAQLRISMRRM 736

Query: 724 NVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +V KVPGQSWIEIKDKVHVFLAED+LHPERGKIYTMLEELMLQ LDD CDP+++
Sbjct: 737 DVGKVPGQSWIEIKDKVHVFLAEDNLHPERGKIYTMLEELMLQTLDDSCDPLQM 790

BLAST of Lsi03G020440 vs. NCBI nr
Match: XP_023526527.1 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023526528.1 pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023526529.1 pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 674/775 (86.97%), Postives = 720/775 (92.90%), Query Frame = 0

Query: 3   TSDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAFSPFPVTDHYSHDDKLISLCKK 62
           +S+AIVFFNP SP  S KNFKPQLHLALSHIRLN+Q+AFSP PV +H S+DD +ISLCKK
Sbjct: 44  SSEAIVFFNPCSPANSIKNFKPQLHLALSHIRLNSQIAFSPSPVAEH-SYDDNIISLCKK 103

Query: 63  NCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMIL 122
             HREALQAFDIFQKCSNSPL SITYTHLI+ACSSLRSLEHGRKIH HM TFNYQPD+IL
Sbjct: 104 KLHREALQAFDIFQKCSNSPLNSITYTHLIHACSSLRSLEHGRKIHCHMSTFNYQPDLIL 163

Query: 123 QNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGY 182
           QNHILNMYGKCGSLKEARNIFDAMPLKN VSWTSMISGYSHYGQ+DNAI LYVQMLRSG+
Sbjct: 164 QNHILNMYGKCGSLKEARNIFDAMPLKNAVSWTSMISGYSHYGQDDNAITLYVQMLRSGH 223

Query: 183 IPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAKN 242
           IPDHFTFGSVVKSCSGLDD MLARQLHAHVLKSEFG + IAQNALISMYTK SQ+ADA N
Sbjct: 224 IPDHFTFGSVVKSCSGLDDLMLARQLHAHVLKSEFGGNPIAQNALISMYTKFSQIADATN 283

Query: 243 VFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKLL 302
           VFSHII K+LISWGSMIAGFSQLGYE+EALCHFREMLSQP+YQPNEFVFGSAFSACS L 
Sbjct: 284 VFSHIITKNLISWGSMIAGFSQLGYEIEALCHFREMLSQPIYQPNEFVFGSAFSACSNLS 343

Query: 303 EPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSIIA 362
           EPN GRQIHGLCIKFGLG D FAGCSLCDMYAKCGFLGSARTVF  IEKPDLVAWN+IIA
Sbjct: 344 EPNCGRQIHGLCIKFGLGRDRFAGCSLCDMYAKCGFLGSARTVFCQIEKPDLVAWNAIIA 403

Query: 363 GFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFDL 422
           GFASVGDAKESLSFFS+MRHTGL  NDVTVLSLLCACSEP+MLNQGMQVHSYIV+ GFDL
Sbjct: 404 GFASVGDAKESLSFFSQMRHTGLASNDVTVLSLLCACSEPMMLNQGMQVHSYIVKTGFDL 463

Query: 423 DIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKLM 482
           ++PVCN LLSMYSKCS+LND+L+IFE IGNKAD+VSWNTMLT C  QNQAGEVLRL KLM
Sbjct: 464 EVPVCNGLLSMYSKCSDLNDSLKIFEDIGNKADVVSWNTMLTVCRLQNQAGEVLRLMKLM 523

Query: 483 LASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGSL 542
           LAS IK D VTLTNVLVSSGQIASYEVGSQVHCFIMKSG NLDTSVSNALINMY KCGSL
Sbjct: 524 LASRIKSDRVTLTNVLVSSGQIASYEVGSQVHCFIMKSGQNLDTSVSNALINMYMKCGSL 583

Query: 543 GCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTAC 602
           GCA+KMFDSID+PD+ISWSSLIVGYAQAGCGEEAF+LFRTMRGLG++PNEITF+GILTAC
Sbjct: 584 GCAQKMFDSIDDPDVISWSSLIVGYAQAGCGEEAFKLFRTMRGLGIRPNEITFLGILTAC 643

Query: 603 SHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIVV 662
            HIGMVEEGL+LYRTMQE+  ISPTKEH SC+VDLLARAGCLD AEDFIK MPF+PDIVV
Sbjct: 644 CHIGMVEEGLRLYRTMQEQDGISPTKEHYSCIVDLLARAGCLDAAEDFIKKMPFEPDIVV 703

Query: 663 WKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMRQ 722
           W TLLAACK+HGNLEVGKRAAENVLK DPSNSAALVMLCNIHASSG WKDFA+ R SMR+
Sbjct: 704 WMTLLAACKLHGNLEVGKRAAENVLKNDPSNSAALVMLCNIHASSGRWKDFARIRRSMRR 763

Query: 723 MNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           M+VSKVPGQSWIEIKD+VHVF AEDSLHPERG+IYTMLEELMLQVLDD CDP+++
Sbjct: 764 MDVSKVPGQSWIEIKDRVHVFFAEDSLHPERGRIYTMLEELMLQVLDDSCDPLQM 817

BLAST of Lsi03G020440 vs. NCBI nr
Match: XP_022934158.1 (pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like [Cucurbita moschata] >XP_022934159.1 pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like [Cucurbita moschata] >XP_022934160.1 pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 674/776 (86.86%), Postives = 720/776 (92.78%), Query Frame = 0

Query: 3   TSDAIVFFNPFSPTTSTKNFKPQLHLALSHIRLNTQLAF-SPFPVTDHYSHDDKLISLCK 62
           +S+AIVFFNP SP TS KNFKPQL LALSHIR ++ LA   P PVT+H S+DD +ISLCK
Sbjct: 44  SSEAIVFFNPCSPATSIKNFKPQLRLALSHIRFSSLLASPPPSPVTEH-SYDDNIISLCK 103

Query: 63  KNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMI 122
           K  HREALQAFDIFQKCS+SPL SITYTHLI+ACSSLRSLEHGRKIH HM TFNYQPD+I
Sbjct: 104 KKLHREALQAFDIFQKCSSSPLNSITYTHLIHACSSLRSLEHGRKIHCHMSTFNYQPDLI 163

Query: 123 LQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSG 182
           LQNHILNMYGKCGSLKEARNIFDAMPLKN VSWTSMISGYSHYGQ+DNAI LYVQMLRSG
Sbjct: 164 LQNHILNMYGKCGSLKEARNIFDAMPLKNAVSWTSMISGYSHYGQDDNAITLYVQMLRSG 223

Query: 183 YIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADAK 242
           +IPDHFTFGSVVKSCSGLDD MLARQLHAHVLKSEFG + IAQNALISMYTK SQ+ADA 
Sbjct: 224 HIPDHFTFGSVVKSCSGLDDLMLARQLHAHVLKSEFGGNPIAQNALISMYTKFSQIADAT 283

Query: 243 NVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSKL 302
           NVFSHIIIKDLISWGSMIAGFSQLGYE+EALCHFREMLSQ +YQPNEFVFGSAFSACS L
Sbjct: 284 NVFSHIIIKDLISWGSMIAGFSQLGYEIEALCHFREMLSQAIYQPNEFVFGSAFSACSSL 343

Query: 303 LEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSII 362
            EPN GRQIHGLCIKFGLGSD FAGCSLCDMYAKCGFLGSARTVF  IEKPDLVAWN+II
Sbjct: 344 SEPNCGRQIHGLCIKFGLGSDRFAGCSLCDMYAKCGFLGSARTVFCQIEKPDLVAWNAII 403

Query: 363 AGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGFD 422
           AGFASVGDAKESLSFFS+MRHTGL  NDVTVLSLLCACS+P+MLNQGMQVHSYIV+ GFD
Sbjct: 404 AGFASVGDAKESLSFFSQMRHTGLASNDVTVLSLLCACSDPMMLNQGMQVHSYIVKTGFD 463

Query: 423 LDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLTKL 482
           L++PVCN LLSMYSKCS LND+L+IFE IGNKADIVSWNTMLTAC  QNQAGEVLRL KL
Sbjct: 464 LEVPVCNGLLSMYSKCSVLNDSLKIFEDIGNKADIVSWNTMLTACRLQNQAGEVLRLMKL 523

Query: 483 MLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKCGS 542
           MLASHIK D VTLTNVLVSSGQIASYEVGSQVHCFIMKSG NLDTSVSNALINMY KCGS
Sbjct: 524 MLASHIKSDRVTLTNVLVSSGQIASYEVGSQVHCFIMKSGQNLDTSVSNALINMYMKCGS 583

Query: 543 LGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGILTA 602
           LGCARKMFDSI++PD+ISWSSLIVGYAQAGCGEEAFELFRTMRGLG++PNEITF+GILTA
Sbjct: 584 LGCARKMFDSINDPDVISWSSLIVGYAQAGCGEEAFELFRTMRGLGIRPNEITFIGILTA 643

Query: 603 CSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDPDIV 662
           C HIGMVEEGL+LYRTMQE+  ISPTKEHCSC+VDLLARAGCLD AEDFIK MPF+PDIV
Sbjct: 644 CCHIGMVEEGLRLYRTMQEQDGISPTKEHCSCIVDLLARAGCLDAAEDFIKKMPFEPDIV 703

Query: 663 VWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRSSMR 722
           VW TLLAACK+HGNLEVGKRAAENVLK DPSNSAALVMLCNIHASSG WKDFA+ R SMR
Sbjct: 704 VWMTLLAACKLHGNLEVGKRAAENVLKNDPSNSAALVMLCNIHASSGHWKDFARLRRSMR 763

Query: 723 QMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDPIKV 778
           +M+VSKVPGQSWIEIKD+VHVF AEDSLHPERG+IYTMLEELM+Q+LDD CDP+++
Sbjct: 764 RMDVSKVPGQSWIEIKDRVHVFFAEDSLHPERGRIYTMLEELMVQILDDSCDPLQM 818

BLAST of Lsi03G020440 vs. TAIR 10
Match: AT3G53360.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 896.0 bits (2314), Expect = 2.2e-260
Identity = 426/722 (59.00%), Postives = 552/722 (76.45%), Query Frame = 0

Query: 53  DDKLISLCKKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHML 112
           +D + SLCK N +REAL+AFD  QK S+  ++  TY  LI ACSS RSL  GRKIH H+L
Sbjct: 35  NDHINSLCKSNFYREALEAFDFAQKNSSFKIRLRTYISLICACSSSRSLAQGRKIHDHIL 94

Query: 113 TFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIA 172
             N + D IL NHIL+MYGKCGSL++AR +FD MP +N+VS+TS+I+GYS  GQ   AI 
Sbjct: 95  NSNCKYDTILNNHILSMYGKCGSLRDAREVFDFMPERNLVSYTSVITGYSQNGQGAEAIR 154

Query: 173 LYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYT 232
           LY++ML+   +PD F FGS++K+C+   D  L +QLHA V+K E  SHLIAQNALI+MY 
Sbjct: 155 LYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGKQLHAQVIKLESSSHLIAQNALIAMYV 214

Query: 233 KLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFG 292
           + +QM+DA  VF  I +KDLISW S+IAGFSQLG+E EAL H +EMLS  V+ PNE++FG
Sbjct: 215 RFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLGFEFEALSHLKEMLSFGVFHPNEYIFG 274

Query: 293 SAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKP 352
           S+  ACS LL P+YG QIHGLCIK  L  +  AGCSLCDMYA+CGFL SAR VF  IE+P
Sbjct: 275 SSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAGCSLCDMYARCGFLNSARRVFDQIERP 334

Query: 353 DLVAWNSIIAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVH 412
           D  +WN IIAG A+ G A E++S FS+MR +G +P+ +++ SLLCA ++P+ L+QGMQ+H
Sbjct: 335 DTASWNVIIAGLANNGYADEAVSVFSQMRSSGFIPDAISLRSLLCAQTKPMALSQGMQIH 394

Query: 413 SYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQA 472
           SYI++ GF  D+ VCNSLL+MY+ CS+L     +FE   N AD VSWNT+LTACLQ  Q 
Sbjct: 395 SYIIKWGFLADLTVCNSLLTMYTFCSDLYCCFNLFEDFRNNADSVSWNTILTACLQHEQP 454

Query: 473 GEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNAL 532
            E+LRL KLML S  +PDH+T+ N+L    +I+S ++GSQVHC+ +K+GL  +  + N L
Sbjct: 455 VEMLRLFKLMLVSECEPDHITMGNLLRGCVEISSLKLGSQVHCYSLKTGLAPEQFIKNGL 514

Query: 533 INMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNE 592
           I+MY KCGSLG AR++FDS+DN D++SWS+LIVGYAQ+G GEEA  LF+ M+  G++PN 
Sbjct: 515 IDMYAKCGSLGQARRIFDSMDNRDVVSWSTLIVGYAQSGFGEEALILFKEMKSAGIEPNH 574

Query: 593 ITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIK 652
           +TFVG+LTACSH+G+VEEGLKLY TMQ E+ ISPTKEHCSC+VDLLARAG L+ AE FI 
Sbjct: 575 VTFVGVLTACSHVGLVEEGLKLYATMQTEHGISPTKEHCSCVVDLLARAGRLNEAERFID 634

Query: 653 HMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKD 712
            M  +PD+VVWKTLL+ACK  GN+ + ++AAEN+LKIDP NS A V+LC++HASSG W++
Sbjct: 635 EMKLEPDVVVWKTLLSACKTQGNVHLAQKAAENILKIDPFNSTAHVLLCSMHASSGNWEN 694

Query: 713 FAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGC 772
            A  RSSM++ +V K+PGQSWIEI+DK+H+F AED  HPER  IYT+L  +  Q+LD+ C
Sbjct: 695 AALLRSSMKKHDVKKIPGQSWIEIEDKIHIFFAEDIFHPERDDIYTVLHNIWSQMLDE-C 754

Query: 773 DP 775
           +P
Sbjct: 755 NP 755

BLAST of Lsi03G020440 vs. TAIR 10
Match: AT3G03580.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 471.9 bits (1213), Expect = 1.0e-132
Identity = 237/730 (32.47%), Postives = 401/730 (54.93%), Query Frame = 0

Query: 45  PVTDHYSHDDKLISLCKKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHG 104
           P  + Y  +  + +  K     EAL+ +   ++   SP K  T+  +I AC+ L   E G
Sbjct: 67  PAKNVYLWNSIIRAFSKNGLFPEALEFYGKLRESKVSPDK-YTFPSVIKACAGLFDAEMG 126

Query: 105 RKIHRHMLTFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHY 164
             ++  +L   ++ D+ + N +++MY + G L  AR +FD MP++++VSW S+ISGYS +
Sbjct: 127 DLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSH 186

Query: 165 GQEDNAIALYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQ 224
           G  + A+ +Y ++  S  +PD FT  SV+ +   L      + LH   LKS   S ++  
Sbjct: 187 GYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQGLHGFALKSGVNSVVVVN 246

Query: 225 NALISMYTKLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVY 284
           N L++MY K  +  DA+ VF  + ++D +S+ +MI G+ +L    E++  F E L Q  +
Sbjct: 247 NGLVAMYLKFRRPTDARRVFDEMDVRDSVSYNTMICGYLKLEMVEESVRMFLENLDQ--F 306

Query: 285 QPNEFVFGSAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSART 344
           +P+     S   AC  L + +  + I+   +K G   +      L D+YAKCG + +AR 
Sbjct: 307 KPDLLTVSSVLRACGHLRDLSLAKYIYNYMLKAGFVLESTVRNILIDVYAKCGDMITARD 366

Query: 345 VFYHIEKPDLVAWNSIIAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVM 404
           VF  +E  D V+WNSII+G+   GD  E++  F  M       + +T L L+   +    
Sbjct: 367 VFNSMECKDTVSWNSIISGYIQSGDLMEAMKLFKMMMIMEEQADHITYLMLISVSTRLAD 426

Query: 405 LNQGMQVHSYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLT 464
           L  G  +HS  ++ G  +D+ V N+L+ MY+KC  + D+L+IF  +G   D V+WNT+++
Sbjct: 427 LKFGKGLHSNGIKSGICIDLSVSNALIDMYAKCGEVGDSLKIFSSMGT-GDTVTWNTVIS 486

Query: 465 ACLQQNQAGEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNL 524
           AC++       L++T  M  S + PD  T    L     +A+  +G ++HC +++ G   
Sbjct: 487 ACVRFGDFATGLQVTTQMRKSEVVPDMATFLVTLPMCASLAAKRLGKEIHCCLLRFGYES 546

Query: 525 DTSVSNALINMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMR 584
           +  + NALI MY+KCG L  + ++F+ +   D+++W+ +I  Y   G GE+A E F  M 
Sbjct: 547 ELQIGNALIEMYSKCGCLENSSRVFERMSRRDVVTWTGMIYAYGMYGEGEKALETFADME 606

Query: 585 GLGVKPNEITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCL 644
             G+ P+ + F+ I+ ACSH G+V+EGL  +  M+  Y I P  EH +C+VDLL+R+  +
Sbjct: 607 KSGIVPDSVVFIAIIYACSHSGLVDEGLACFEKMKTHYKIDPMIEHYACVVDLLSRSQKI 666

Query: 645 DGAEDFIKHMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIH 704
             AE+FI+ MP  PD  +W ++L AC+  G++E  +R +  +++++P +    ++  N +
Sbjct: 667 SKAEEFIQAMPIKPDASIWASVLRACRTSGDMETAERVSRRIIELNPDDPGYSILASNAY 726

Query: 705 ASSGCWKDFAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELM 764
           A+   W   +  R S++  +++K PG SWIE+   VHVF + D   P+   IY  LE L 
Sbjct: 727 AALRKWDKVSLIRKSLKDKHITKNPGYSWIEVGKNVHVFSSGDDSAPQSEAIYKSLEILY 786

Query: 765 LQVLDDGCDP 775
             +  +G  P
Sbjct: 787 SLMAKEGYIP 792

BLAST of Lsi03G020440 vs. TAIR 10
Match: AT3G49170.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 471.1 bits (1211), Expect = 1.7e-132
Identity = 248/719 (34.49%), Postives = 410/719 (57.02%), Query Frame = 0

Query: 66  REALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDMILQNH 125
           R A+ A D+  +    P+ S+T++ L+ +C   R    G+ +H  ++ F+ +PD +L N 
Sbjct: 43  RGAVSALDLMARDGIRPMDSVTFSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNS 102

Query: 126 ILNMYGKCGSLKEARNIFDAM---PLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRSGY 185
           ++++Y K G   +A ++F+ M     ++VVSW++M++ Y + G+E +AI ++V+ L  G 
Sbjct: 103 LISLYSKSGDSAKAEDVFETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGL 162

Query: 186 IPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKS-EFGSHLIAQNALISMYTK-LSQMADA 245
           +P+ + + +V+++CS  D   + R     ++K+  F S +    +LI M+ K  +   +A
Sbjct: 163 VPNDYCYTAVIRACSNSDFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENA 222

Query: 246 KNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSK 305
             VF  +   ++++W  MI    Q+G+  EA+  F +M+    ++ ++F   S FSAC++
Sbjct: 223 YKVFDKMSELNVVTWTLMITRCMQMGFPREAIRFFLDMVLSG-FESDKFTLSSVFSACAE 282

Query: 306 LLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKC---GFLGSARTVFYHIEKPDLVAW 365
           L   + G+Q+H   I+ GL  D+   CSL DMYAKC   G +   R VF  +E   +++W
Sbjct: 283 LENLSLGKQLHSWAIRSGLVDDV--ECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSW 342

Query: 366 NSIIAGF-ASVGDAKESLSFFSRMRHTGLV-PNDVTVLSLLCACSEPVMLNQGMQVHSYI 425
            ++I G+  +   A E+++ FS M   G V PN  T  S   AC        G QV    
Sbjct: 343 TALITGYMKNCNLATEAINLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQA 402

Query: 426 VRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEV 485
            + G   +  V NS++SM+ K   + DA + FE +  K ++VS+NT L    +     + 
Sbjct: 403 FKRGLASNSSVANSVISMFVKSDRMEDAQRAFESLSEK-NLVSYNTFLDGTCRNLNFEQA 462

Query: 486 LRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINM 545
            +L   +    +     T  ++L     + S   G Q+H  ++K GL+ +  V NALI+M
Sbjct: 463 FKLLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISM 522

Query: 546 YTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITF 605
           Y+KCGS+  A ++F+ ++N ++ISW+S+I G+A+ G      E F  M   GVKPNE+T+
Sbjct: 523 YSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTY 582

Query: 606 VGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMP 665
           V IL+ACSH+G+V EG + + +M E++ I P  EH +CMVDLL RAG L  A +FI  MP
Sbjct: 583 VAILSACSHVGLVSEGWRHFNSMYEDHKIKPKMEHYACMVDLLCRAGLLTDAFEFINTMP 642

Query: 666 FDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQ 725
           F  D++VW+T L AC+VH N E+GK AA  +L++DP+  AA + L NI+A +G W++  +
Sbjct: 643 FQADVLVWRTFLGACRVHSNTELGKLAARKILELDPNEPAAYIQLSNIYACAGKWEESTE 702

Query: 726 YRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDDGCDP 775
            R  M++ N+ K  G SWIE+ DK+H F   D+ HP   +IY  L+ L+ ++   G  P
Sbjct: 703 MRRKMKERNLVKEGGCSWIEVGDKIHKFYVGDTAHPNAHQIYDELDRLITEIKRCGYVP 757

BLAST of Lsi03G020440 vs. TAIR 10
Match: AT3G09040.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 469.2 bits (1206), Expect = 6.6e-132
Identity = 245/712 (34.41%), Postives = 390/712 (54.78%), Query Frame = 0

Query: 61   KKNCHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGRKIHRHMLTFNYQPDM 120
            K+ C   A++ F   +K S    +S T   +++A   + +L+ G  +H   +      ++
Sbjct: 304  KRGCETVAIEYFFNMRKSSVKSTRS-TLGSVLSAIGIVANLDLGLVVHAEAIKLGLASNI 363

Query: 121  ILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNAIALYVQMLRS 180
             + + +++MY KC  ++ A  +F+A+  KN V W +MI GY+H G+    + L++ M  S
Sbjct: 364  YVGSSLVSMYSKCEKMEAAAKVFEALEEKNDVFWNAMIRGYAHNGESHKVMELFMDMKSS 423

Query: 181  GYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISMYTKLSQMADA 240
            GY  D FTF S++ +C+   D  +  Q H+ ++K +   +L   NAL+ MY K   + DA
Sbjct: 424  GYNIDDFTFTSLLSTCAASHDLEMGSQFHSIIIKKKLAKNLFVGNALVDMYAKCGALEDA 483

Query: 241  KNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFVFGSAFSACSK 300
            + +F  +  +D ++W ++I  + Q   E EA   F+ M    +         S   AC+ 
Sbjct: 484  RQIFERMCDRDNVTWNTIIGSYVQDENESEAFDLFKRMNLCGIVSDGA-CLASTLKACTH 543

Query: 301  LLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIEKPDLVAWNSI 360
            +     G+Q+H L +K GL  DL  G SL DMY+KCG +  AR VF  + +  +V+ N++
Sbjct: 544  VHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMYSKCGIIKDARKVFSSLPEWSVVSMNAL 603

Query: 361  IAGFASVGDAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQGMQVHSYIVRMGF 420
            IAG+ S  + +E++  F  M   G+ P+++T  +++ AC +P  L  G Q H  I + GF
Sbjct: 604  IAGY-SQNNLEEAVVLFQEMLTRGVNPSEITFATIVEACHKPESLTLGTQFHGQITKRGF 663

Query: 421  DLDIPVCN-SLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACLQQNQAGEVLRLT 480
              +      SLL MY     + +A  +F  + +   IV W  M++   Q     E L+  
Sbjct: 664  SSEGEYLGISLLGMYMNSRGMTEACALFSELSSPKSIVLWTGMMSGHSQNGFYEEALKFY 723

Query: 481  KLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTSVSNALINMYTKC 540
            K M    + PD  T   VL     ++S   G  +H  I     +LD   SN LI+MY KC
Sbjct: 724  KEMRHDGVLPDQATFVTVLRVCSVLSSLREGRAIHSLIFHLAHDLDELTSNTLIDMYAKC 783

Query: 541  GSLGCARKMFDSI-DNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLGVKPNEITFVGI 600
            G +  + ++FD +    +++SW+SLI GYA+ G  E+A ++F +MR   + P+EITF+G+
Sbjct: 784  GDMKGSSQVFDEMRRRSNVVSWNSLINGYAKNGYAEDALKIFDSMRQSHIMPDEITFLGV 843

Query: 601  LTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGAEDFIKHMPFDP 660
            LTACSH G V +G K++  M  +Y I    +H +CMVDLL R G L  A+DFI+     P
Sbjct: 844  LTACSHAGKVSDGRKIFEMMIGQYGIEARVDHVACMVDLLGRWGYLQEADDFIEAQNLKP 903

Query: 661  DIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASSGCWKDFAQYRS 720
            D  +W +LL AC++HG+   G+ +AE +++++P NS+A V+L NI+AS GCW+     R 
Sbjct: 904  DARLWSSLLGACRIHGDDIRGEISAEKLIELEPQNSSAYVLLSNIYASQGCWEKANALRK 963

Query: 721  SMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQVLDD 771
             MR   V KVPG SWI+++ + H+F A D  H E GKI   LE+L   + DD
Sbjct: 964  VMRDRGVKKVPGYSWIDVEQRTHIFAAGDKSHSEIGKIEMFLEDLYDLMKDD 1012

BLAST of Lsi03G020440 vs. TAIR 10
Match: AT4G39530.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 465.7 bits (1197), Expect = 7.3e-131
Identity = 249/720 (34.58%), Postives = 408/720 (56.67%), Query Frame = 0

Query: 56  LISLCKKN-CHREALQAFDIFQKCSNSPLKSITYTHLINACSSLRSLEHGR----KIHRH 115
           ++S C  +  + E+L  F  F +           +  I ACS L     GR    ++   
Sbjct: 116 MVSACNHHGIYEESLVVFLEFWRTRKDSPNEYILSSFIQACSGLDG--RGRWMVFQLQSF 175

Query: 116 MLTFNYQPDMILQNHILNMYGKCGSLKEARNIFDAMPLKNVVSWTSMISGYSHYGQEDNA 175
           ++   +  D+ +   +++ Y K G++  AR +FDA+P K+ V+WT+MISG    G+   +
Sbjct: 176 LVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVS 235

Query: 176 IALYVQMLRSGYIPDHFTFGSVVKSCSGLDDFMLARQLHAHVLKSEFGSHLIAQNALISM 235
           + L+ Q++    +PD +   +V+ +CS L      +Q+HAH+L+          N LI  
Sbjct: 236 LQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDS 295

Query: 236 YTKLSQMADAKNVFSHIIIKDLISWGSMIAGFSQLGYELEALCHFREMLSQPVYQPNEFV 295
           Y K  ++  A  +F+ +  K++ISW ++++G+ Q     EA+  F  M S+   +P+ + 
Sbjct: 296 YVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSM-SKFGLKPDMYA 355

Query: 296 FGSAFSACSKLLEPNYGRQIHGLCIKFGLGSDLFAGCSLCDMYAKCGFLGSARTVFYHIE 355
             S  ++C+ L    +G Q+H   IK  LG+D +   SL DMYAKC  L  AR VF    
Sbjct: 356 CSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFA 415

Query: 356 KPDLVAWNSIIAGFASVG---DAKESLSFFSRMRHTGLVPNDVTVLSLLCACSEPVMLNQ 415
             D+V +N++I G++ +G   +  E+L+ F  MR   + P+ +T +SLL A +    L  
Sbjct: 416 AADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGL 475

Query: 416 GMQVHSYIVRMGFDLDIPVCNSLLSMYSKCSNLNDALQIFEGIGNKADIVSWNTMLTACL 475
             Q+H  + + G +LDI   ++L+ +YS C  L D+  +F+ +  K D+V WN+M    +
Sbjct: 476 SKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDEMKVK-DLVIWNSMFAGYV 535

Query: 476 QQNQAGEVLRLTKLMLASHIKPDHVTLTNVLVSSGQIASYEVGSQVHCFIMKSGLNLDTS 535
           QQ++  E L L   +  S  +PD  T  N++ ++G +AS ++G + HC ++K GL  +  
Sbjct: 536 QQSENEEALNLFLELQLSRERPDEFTFANMVTAAGNLASVQLGQEFHCQLLKRGLECNPY 595

Query: 536 VSNALINMYTKCGSLGCARKMFDSIDNPDIISWSSLIVGYAQAGCGEEAFELFRTMRGLG 595
           ++NAL++MY KCGS   A K FDS  + D++ W+S+I  YA  G G++A ++   M   G
Sbjct: 596 ITNALLDMYAKCGSPEDAHKAFDSAASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEG 655

Query: 596 VKPNEITFVGILTACSHIGMVEEGLKLYRTMQEEYDISPTKEHCSCMVDLLARAGCLDGA 655
           ++PN ITFVG+L+ACSH G+VE+GLK +  M   + I P  EH  CMV LL RAG L+ A
Sbjct: 656 IEPNYITFVGVLSACSHAGLVEDGLKQFELML-RFGIEPETEHYVCMVSLLGRAGRLNKA 715

Query: 656 EDFIKHMPFDPDIVVWKTLLAACKVHGNLEVGKRAAENVLKIDPSNSAALVMLCNIHASS 715
            + I+ MP  P  +VW++LL+ C   GN+E+ + AAE  +  DP +S +  ML NI+AS 
Sbjct: 716 RELIEKMPTKPAAIVWRSLLSGCAKAGNVELAEHAAEMAILSDPKDSGSFTMLSNIYASK 775

Query: 716 GCWKDFAQYRSSMRQMNVSKVPGQSWIEIKDKVHVFLAEDSLHPERGKIYTMLEELMLQV 768
           G W +  + R  M+   V K PG+SWI I  +VH+FL++D  H +  +IY +L++L++Q+
Sbjct: 776 GMWTEAKKVRERMKVEGVVKEPGRSWIGINKEVHIFLSKDKSHCKANQIYEVLDDLLVQI 830

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFI13.1e-25959.00Pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Arabidop... [more]
Q9SS601.4e-13132.47Pentatricopeptide repeat-containing protein At3g03580 OS=Arabidopsis thaliana OX... [more]
Q5G1T12.5e-13134.49Pentatricopeptide repeat-containing protein At3g49170, chloroplastic OS=Arabidop... [more]
Q9SS839.3e-13134.41Pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Arabidop... [more]
Q9SVA51.0e-12934.58Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A0A0LDF30.0e+0091.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736910 PE=4 SV=1[more]
A0A5A7TPL00.0e+0091.09Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B6840.0e+0091.09pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Cucumis ... [more]
A0A6J1F1S70.0e+0086.86pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like OS=Cuc... [more]
A0A6J1J9110.0e+0086.45pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like OS=Cuc... [more]
Match NameE-valueIdentityDescription
XP_038903429.10.0e+0093.43pentatricopeptide repeat-containing protein At3g53360, mitochondrial isoform X1 ... [more]
XP_004137966.10.0e+0091.34pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucumis sa... [more]
XP_008442662.10.0e+0091.09PREDICTED: pentatricopeptide repeat-containing protein At3g53360, mitochondrial ... [more]
XP_023526527.10.0e+0086.97pentatricopeptide repeat-containing protein At3g53360, mitochondrial [Cucurbita ... [more]
XP_022934158.10.0e+0086.86pentatricopeptide repeat-containing protein At3g53360, mitochondrial-like [Cucur... [more]
Match NameE-valueIdentityDescription
AT3G53360.12.2e-26059.00Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G03580.11.0e-13232.47Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G49170.11.7e-13234.49Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G09040.16.6e-13234.41Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G39530.17.3e-13134.58Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 86..119
e-value: 7.0E-4
score: 17.6
coord: 594..627
e-value: 0.0015
score: 16.6
coord: 457..490
e-value: 2.2E-4
score: 19.2
coord: 558..592
e-value: 3.7E-7
score: 27.9
coord: 355..388
e-value: 1.3E-5
score: 23.0
coord: 152..185
e-value: 2.8E-6
score: 25.1
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 427..448
e-value: 0.17
score: 12.2
coord: 457..484
e-value: 0.05
score: 13.9
coord: 253..280
e-value: 0.0041
score: 17.3
coord: 530..551
e-value: 0.76
score: 10.2
coord: 86..114
e-value: 0.094
score: 13.0
coord: 631..654
e-value: 0.52
score: 10.7
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 555..603
e-value: 1.4E-9
score: 38.0
coord: 352..399
e-value: 5.0E-8
score: 33.0
coord: 149..196
e-value: 1.0E-9
score: 38.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 455..489
score: 9.909073
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 353..387
score: 11.706726
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 84..118
score: 9.076014
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 556..590
score: 12.320559
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 150..184
score: 11.783455
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 52..216
e-value: 3.5E-33
score: 117.3
coord: 574..768
e-value: 7.4E-29
score: 103.1
coord: 270..403
e-value: 2.8E-22
score: 81.5
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 404..511
e-value: 2.9E-18
score: 67.8
NoneNo IPR availablePANTHERPTHR24015:SF1672OS06G0506100 PROTEINcoord: 284..410
coord: 386..759
coord: 51..283
NoneNo IPR availablePANTHERPTHR24015OS07G0578800 PROTEIN-RELATEDcoord: 284..410
coord: 386..759
coord: 51..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G020440.1Lsi03G020440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009451 RNA modification
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0005515 protein binding
molecular_function GO:0003723 RNA binding