Lsi03G009000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G009000
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	tRNA (Guanine(10)-N2)-methyltransferase homolog
Location	chr03: 16449605 .. 16453834 (+)
RNA-Seq Expression	Lsi03G009000
Synteny	Lsi03G009000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAGTTTTAGGAAAAGAATTTAGATGGAGAAGCACACATGACTTTGCCTCACTTTTTTGAAAGGGTGATTTTTAAAACACCCACTTGCATTTATCTCAACTAATCGTATGGGACAATTGCCCATACATATAACATTTGGTTGTCAATAAAACTCGTAGGATTAAATCCTTGGTAGGTGGCCAGTGACCACTATAACTTGGAACCATTTCCTTTCGAGTCTTTTTACCTTTTTTATTGGTTCTAATGACTATTAGGTTAATTATTGATCTATTATTTGATATAGGATGTTCTCCTAGGGGATTCAGTCTGTTTACTTTATTTGTTTCCCCTTCTTGTGTAATTGCTGTCACTAGTTCTCAAATATGGAATTACTTGTCCAGGGACTTGCAATTATATTTTCATCCCTTGCCCCTTCAGCCTAAAAGTGTGATATTTTATATTTCATTATTCAATAATTCTTTCTCGTGCACCTTCAAATTCCGTTTGTAATATAACATGATCTTGAATTATGTATTTTTTAATGATTCTTGAATGATGTTTTAAATATAAAAAGTGATATTTTTTATTTCATTATTCAATGATTCTTTTTGTGCACCTTCAAATTCTGTTCATAATATAACATTATCTTGAATGATGTGTTATAGTACAGCCAAAGTAAGCGAAGCCAAGACTCATGCGAGTTAATGCAGTAGTTACTCATTTGAGGAATGTGATAATAAGGAACATGCAATCTTTTAAAGATGTACACAAGTTGAGGAGAGATTCTTGATTAGCTTAATTATAAGCTTCTTGTTTGTGTGCACCGTTAAAATTCCTTTTTTAATTCTACTCTCTTTTTAGCTACATGGAGTTCATTTTTCGTAACTTCTATTAAGTAGTCCTTTTCACCCTACCACTGAAAAGCATTTTACTTGTATTTTTTGGCTTATTTTTTCAAATCATAATGCTTGCAACTTGATAGAATTCGACTTTGATGGGTTGTTTGAAAGTTGTCACCAGGCACACAAGGAAAGCGATTTGGCGAAAGCAACACATCCTTCCGTAGTAGAAGATTTTAATTATTCATTGTTGTGATGCTTTGTTACAAGTTGTAGCATTATATTTGCTATCCTATCTTATATCTAATAAGGCTTGGTTGGTTATTCTCCTACTGATATTGTCTAATCCTGGTTCTGATGCATTTATACAACTTGCTTTTCTGCCCAGATGTTTGATGCAATCATTTGTGACCCTCCTTATGGTGTGCGTGCCGGAGGGCGTAAATCCGGTGGTCGGAAATTGTTAAAAGGAACAGTTGCTCCTTATACCGTTCCAGATGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTTGCTGCCAAGATGCTTGTGATGGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATTGTGTGTTTAACCAATTCCCTGAACATCCTTGCTTTACTTTAATCAGTTCTTCCGAGCAGATACTGAGCTCTCGATACAGCCGGGTGTTGCTAACTATGGTAAAAAGAAGTTCATACACTGAAGAAATGGCAGAGGCAGCAAGATTAAAACATGAAGAATTCAGGGAGAACCATTTGAAGTGGTTAGAAGATGGTAATCTCCATTCTGCAGTTTTCAGTCCCTCCACTGATCCCCAGCTTGCAGTTGCTGGGAATTGCAATGTGAGTAAAGACCCAAAACCAAAATACAGAGGAAAATATGTATAAATTGCAACTTTTTGTAGGTGTTGCTTTGCTAATTCTCTGAATTTTCTCTAAGCCCCATGGATTTTGGTTATGATGAACTTGGAAAATAGTAGTATTTGAGTTTCTTTTTTTTTTTTTTTTTTTTTGACTTATAAAAATGTAGGTTAAATGACAAGTTTAGTCGTTAAATTTGATTTCTAAATTTTTATTCTTGAATTTTATTCATTTCTAATGTCTTTCAACTTTCAATTATATTTTCTTTGCAAAGAAAAAAAAAATCAATTGTATTTAATAGGTCTGTAACCTTAAAATTTTTAAAATTAATTGTGTAACTAAATTTAAATTTGAACTTTATGTTTAATGGGTTCTAACTTTCAATTGGAAAAAAAGGTTAAATAGGGTACTTAATTTCATTTTTTAATTTAAGATAAAACAGTGTATTTTTTTAGATTTTGTAGAAATAACCTTGTAAATGAAAAATATTATGTTTTGCACCATTGGACTACTTATCTAATTTCAAAACTTTAATCTAAATTAAAATATTATTATTATTTAATTAAAAATATCGTATTATTTACCTTCAACCTATGGACAGTGGCCAAAAAGTTTATTAAAGGCACAAAAAAAAAAAATGTTGAAGGCTCAAACTTCTTGATTTTAGTGATAGATAGTGATAAGAAGTCTAACTGATTAAATGGGTCAATGCATGTATCTACCTTAACAATGCATTTTCAAAATAAGTTAATTTTGATCAACCTAAATTTAGGGCGTATTTGGAATACATTTTTAAGTGTTTAATTTAAAAAATAAATCATTTTAGAAAAAATTGGAGTGTTTGATAACCACTCAAAATAGGTTTTGAATTATATTTTAAACCTTTTTATCAAAAGAGTTTAAATACAAATGAGTTTTTTGAAACAATTTTTTCTCAAGTCAATTCAAATGAGCCCAAGCAAACTTTAAATTCTTTTAAGCATTTCTTAACCCCTCCTAAATTTGGGTGTTGCTGTTTTATGTATGTTAAACTCTTATAAACTCTTATATTGGTTTTTTTTTTTTGTTATTTTAATTATATCAAATACTATATTTCTTACTAATAGGCGTATTATTTTTTTTAGTAATAAGTAGGGGTAAGAAGATTTGAACCACAGATCTCGTGATTTATACACTTGTATGCCAATTGAGCTATGCTCATTTTGACCACTGATATACTTATTTGATACTTTATTTGTTTACGGTTTGTATTTAGTGATAACTTGATAGTTTGTTTTTACATATAACCACATTGATAATTTGAGGCCAAAGAGACAACCCAAACTTATAGTATGTTAATAGACCCTGATAGAAGTATATAGAAACATCAATGATAGAATTATAACTACTTTTTTTTTTTTTTTTATAGAAATATGTCACTGATAAGATTCTAATAGAATTATATGTGCAATTTGATAATCAATCTATCAAGGTCTATTAGGATTTCAACCTTTTAATTTGTAGATCCTATAAAGTTTGATTTCTGATTTAATGATCTTTGATTTGTATCTTCGCATAGCATGTTTGTATTTTAGTTTGATCTTGTTATATCACTGATAGATGGTGATATAAGTCTATCACAAATATATTATTTTTATACTTTATTTGTTTGAGGTTACGTTGCGCCTAATGTTGCACAATGTTCAACACTAGCCTTAGGGACATTTTCGTTTCTTGAGTCCAATTGCTCTGCTAGGCATCTAAATGCATCGTTTTGACTTGAATCGAACTCAGAAAATCTCAACGAACGCTTAAGCTCGATATTCTACATTAAAGCACATTTAAACATTAAATTCCTAAAAACTAGCTCGATTTAGGCTAAAAAAGATAGCCCTTATGGAGAGTTATCAATATTTATTCATGTATTAGTAATATGTAATCACTAAGATGATTACTTTAACTAGCTAATATCATAGTGTATCAATGATAAACTATAAATAATCAAATATTAGTAAGCTAATATACTTGATTCTTTATTAATGAAGTGTATCAAAGATACTTTGAAAAGGTTTGTTTTCAAATCATGACTACGGGTTAAATCTGTAAGTAGTCCATTCACTAATACCACTAAGGGTGTGCACGGTTCGATTCAGTTCGATTTTAGGCAACACCAAGGATTGAACCGAATTGGTCGGTTTATAAAAAAATGTAAACTGTTGAAAACTGGCTAGGTTCAATTTTGGTTTCTGAACCAGCCAGCCTAATACAATTCAGGCCGGTTCGGTTCGGTTAAAATTGAAGTTTGGACCTTTTTTCTCTCCTCCTGCCTAGTGCCTTCTATTGGTCCTCGTCCCGACGTCCCTACTGAAGCTTATATCATTTTTTTTAAAAAAAGAAATTCCTTCGACTGGTGGACTATTAGTGTCGAACTCGAAGTGCGGTGGCCGATGGCAATAGCTATTAGGGGTGGCGATCTGGCCTGGTGTTGTGTAGTGGAGTCGGAGGGGTGA

mRNA sequence

ATGGAAAAATTCGACTTTGATGGGTTGTTTGAAAGTTGTCACCAGGCACACAAGGAAAGCGATTTGGCGAAAGCAACACATCCTTCCGTAATGTTTGATGCAATCATTTGTGACCCTCCTTATGGTGTGCGTGCCGGAGGGCGTAAATCCGGTGGTCGGAAATTGTTAAAAGGAACAGTTGCTCCTTATACCGTTCCAGATGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTTGCTGCCAAGATGCTTGTGATGGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATTGTGTGTTTAACCAATTCCCTGAACATCCTTGCTTTACTTTAATCAGTTCTTCCGAGCAGATACTGAGCTCTCGATACAGCCGGGTGTTGCTAACTATGGTAAAAAGAAGTTCATACACTGAAGAAATGGCAGAGGCAGCAAGATTAAAACATGAAGAATTCAGGGAGAACCATTTGAAGTGGTTAGAAGATGGTAATCTCCATTCTGCAGTTTTCAGTCCCTCCACTGATCCCCAGCTTGCAGTTGCTGGGAATTGCAATGTGAGTAAAGACCCAAAACCAAAATACAGAGGAAAATATTGTATCAAAGATACTTTGAAAAGAAATTCCTTCGACTGGTGGACTATTAGTGTCGAACTCGAAGTGCGGTGGCCGATGGCAATAGCTATTAGGGGTGGCGATCTGGCCTGGTGTTGTGTAGTGGAGTCGGAGGGGTGA

Coding sequence (CDS)

ATGGAAAAATTCGACTTTGATGGGTTGTTTGAAAGTTGTCACCAGGCACACAAGGAAAGCGATTTGGCGAAAGCAACACATCCTTCCGTAATGTTTGATGCAATCATTTGTGACCCTCCTTATGGTGTGCGTGCCGGAGGGCGTAAATCCGGTGGTCGGAAATTGTTAAAAGGAACAGTTGCTCCTTATACCGTTCCAGATGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTTGCTGCCAAGATGCTTGTGATGGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATTGTGTGTTTAACCAATTCCCTGAACATCCTTGCTTTACTTTAATCAGTTCTTCCGAGCAGATACTGAGCTCTCGATACAGCCGGGTGTTGCTAACTATGGTAAAAAGAAGTTCATACACTGAAGAAATGGCAGAGGCAGCAAGATTAAAACATGAAGAATTCAGGGAGAACCATTTGAAGTGGTTAGAAGATGGTAATCTCCATTCTGCAGTTTTCAGTCCCTCCACTGATCCCCAGCTTGCAGTTGCTGGGAATTGCAATGTGAGTAAAGACCCAAAACCAAAATACAGAGGAAAATATTGTATCAAAGATACTTTGAAAAGAAATTCCTTCGACTGGTGGACTATTAGTGTCGAACTCGAAGTGCGGTGGCCGATGGCAATAGCTATTAGGGGTGGCGATCTGGCCTGGTGTTGTGTAGTGGAGTCGGAGGGGTGA

Protein sequence

MEKFDFDGLFESCHQAHKESDLAKATHPSVMFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRSSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKYRGKYCIKDTLKRNSFDWWTISVELEVRWPMAIAIRGGDLAWCCVVESEG

Homology

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BLAST of Lsi03G009000 vs. ExPASy Swiss-Prot
Match: Q54QA6 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Dictyostelium discoideum OX=44689 GN=trmt11 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.0e-20
Identity = 60/138 (43.48%), Postives = 83/138 (60.14%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           MFD+II DPPYG+RAG RK G ++  K       VP+  R DHIP    YS+ + + DLL
Sbjct: 304 MFDSIITDPPYGIRAGARKIGFKENRKFV----PVPEGLRRDHIPQCIDYSVPDVMADLL 363

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSS-EQILSSRYSRVLLTMVK 150
           +LAAK L++GGRLV++ P     D      P HPC  LI++S  QIL++R+ R L+TM K
Sbjct: 364 ELAAKTLIVGGRLVYWLPT--TPDYKETDLPRHPCLRLITASCLQILTNRWGRRLVTMEK 423

Query: 151 RSSYTEEMAEAARLKHEE 168
              Y + +   + L  E+
Sbjct: 424 IIEYNDSIHNKSLLVQED 435

[Close]

BLAST of Lsi03G009000 vs. ExPASy Swiss-Prot
Match: Q6NS23 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Xenopus laevis OX=8355 GN=trmt11 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 7.9e-18
Identity = 61/156 (39.10%), Postives = 82/156 (52.56%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDL 90
           +FDAII DPPYG+R   RK+G +K ++K  + P          H+P    Y L +   DL
Sbjct: 305 LFDAIITDPPYGIRESTRKTGTQKEIIKTELFP--------ESHVPVQLNYHLSDIFSDL 364

Query: 91  LDLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 150
              A++ LV GGRLV++ PV R E       P HPC  LIS+ EQ+LSS  SR L+TM K
Sbjct: 365 FAFASEFLVTGGRLVYWLPVYRPE-YTEEVLPRHPCLKLISNCEQMLSSHTSRRLITMEK 424

Query: 151 RSSYTEE-----MAEAARLK---HEEFRENHLKWLE 178
              + E+     + E   L    H  FRE +   L+
Sbjct: 425 VKEFEEKYQYAHLGEGQNLPYKGHNSFREKYFSGLK 451

[Close]

BLAST of Lsi03G009000 vs. ExPASy Swiss-Prot
Match: Q6YJI5 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Gallus gallus OX=9031 GN=TRMT11 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.0e-17
Identity = 60/152 (39.47%), Postives = 84/152 (55.26%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRT--DHIPSTAAYSLVECVHD 90
           +FDAII DPPYG+R   R++G +K         +V   +R+  +HI  +++Y L +   D
Sbjct: 302 LFDAIITDPPYGIREATRRTGSQK--------ESVKSSERSTENHIIISSSYHLSDIFFD 361

Query: 91  LLDLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMV 150
           LL  AA+ LVMGGRLV++ P+ R E       P HPC  LIS+ EQ+LSS  SR L+TM 
Sbjct: 362 LLKFAAEYLVMGGRLVYWLPIYRPE-YTEEIVPRHPCLKLISNCEQMLSSHTSRRLITME 421

Query: 151 K--------RSSYTEEMAEAARLKHEEFRENH 173
           K        ++SY  +        H  FRE +
Sbjct: 422 KVKEFKDQDQNSYLSDGQYMPYKGHNSFREKY 444

[Close]

BLAST of Lsi03G009000 vs. ExPASy Swiss-Prot
Match: Q7TNK6 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Rattus norvegicus OX=10116 GN=Trmt11 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.9e-17
Identity = 64/170 (37.65%), Postives = 89/170 (52.35%), Query Frame = 0


Query: 1   MEKFDFDGLFESCHQAHKESDLAKAT-HPSVMFDAIICDPPYGVRAGGRKSGGRK-LLKG 60
           +EKF  D L          SD +K +      FDAII DPPYG+R   R++G +K +LKG
Sbjct: 279 LEKFYLDVLV---------SDASKPSWRKGTYFDAIITDPPYGIRESTRRTGSQKEILKG 338

Query: 61  TVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAKMLVMGGRLVFFYPVLREEDCVFN 120
                   +     H+P +  Y L +   DL++ AA+ LV+GGRLV++ PV   E     
Sbjct: 339 I-------EKCPESHVPVSLTYHLSDMFLDLINFAAETLVLGGRLVYWLPVYTPE-YTEK 398

Query: 121 QFPEHPCFTLISSSEQILSSRYSRVLLTMVKRSSYTEEMAEAARLKHEEF 169
             P HPC  L+S+ EQ LSS  +R L+TM K   + E   E +RL  + F
Sbjct: 399 MVPWHPCLRLVSNCEQKLSSHTARRLITMEKVKEF-ENPDEYSRLLSDHF 430

[Close]

BLAST of Lsi03G009000 vs. ExPASy Swiss-Prot
Match: Q5R962 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT11 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.1e-17
Identity = 63/163 (38.65%), Postives = 87/163 (53.37%), Query Frame = 0


Query: 20  SDLAKAT-HPSVMFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPST 79
           SD +K +      FDAII DPPYG+R   R++G +K + KG       P+     H+P +
Sbjct: 289 SDASKPSWRKGTYFDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPE----SHVPVS 348

Query: 80  AAYSLVECVHDLLDLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILS 139
            +Y L +   DLL+ AA+ LV+GGRLV++ PV   E       P HPC  LIS+ EQ LS
Sbjct: 349 LSYHLSDMFLDLLNFAAETLVLGGRLVYWLPVYTPE-YTEEMVPWHPCLELISNCEQKLS 408

Query: 140 SRYSRVLLTMVK------RSSYTEEMAE--AARLKHEEFRENH 173
           S  SR L+TM K      R  Y+  +++       H  FRE +
Sbjct: 409 SHTSRRLITMEKVKKFENRDQYSHLLSDHFLPYQGHNSFREKY 446

[Close]

BLAST of Lsi03G009000 vs. ExPASy TrEMBL
Match: A0A6J1F3J0 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita moschata OX=3662 GN=LOC111439552 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.0e-97
Identity = 176/184 (95.65%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           +LAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 
Sbjct: 356 NLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKH 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAA+LKHEEFRENHLKWLEDGNLHSAVFSP+ DPQLAVAGNCN +KDPKPKY
Sbjct: 416 SSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. ExPASy TrEMBL
Match: A0A0A0K3X0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G229890 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 5.7e-96
Identity = 175/184 (95.11%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLL
Sbjct: 42  IFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLL 101

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           DLAAKMLVMGGRLVFFYP+LREEDCVFNQFPEHPCFT ISSSEQILSSRYSRVLLTMVKR
Sbjct: 102 DLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTSISSSEQILSSRYSRVLLTMVKR 161

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPS +PQL VAGN NVSKDPKPKY
Sbjct: 162 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNPQLGVAGNGNVSKDPKPKY 221

Query: 211 RGKY 215
           RGKY
Sbjct: 222 RGKY 225

[Close]

BLAST of Lsi03G009000 vs. ExPASy TrEMBL
Match: A0A6J1ICG5 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472466 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 7.5e-96
Identity = 172/184 (93.48%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           +LAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 
Sbjct: 356 NLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKH 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYT+EMAEAA+LKHEEFRENHLKWLEDGNLHSAVF P+ DP+L VAGNCN +KDPKPKY
Sbjct: 416 SSYTKEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFGPTIDPRLTVAGNCNATKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. ExPASy TrEMBL
Match: A0A1S3BG98 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucumis melo OX=3656 GN=LOC103489693 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.7e-95
Identity = 174/184 (94.57%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           DLAAKMLVMGGRLVFFYP+LREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR
Sbjct: 356 DLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEE+AEAARLKHEEFRENHLKWLEDGNLHSAVFSPS + QL VAGN NVSKDPKPKY
Sbjct: 416 SSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNRQLGVAGNGNVSKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. ExPASy TrEMBL
Match: A0A6J1D5Q5 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Momordica charantia OX=3673 GN=LOC111017553 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.7e-92
Identity = 169/184 (91.85%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKG VAPYT PDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 299 IFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTXPDDKRTDHIPSTAAYSLVECVHDLL 358

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           DLAA+MLVMGGRLVFFYPVLREEDC FNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR
Sbjct: 359 DLAARMLVMGGRLVFFYPVLREEDCAFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 418

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           +SYTEE+AEA+RLKHEEFRENHLKWLEDGNLHSAVFSP+ DP+LAVAGN +V +DPKPKY
Sbjct: 419 NSYTEELAEASRLKHEEFRENHLKWLEDGNLHSAVFSPA-DPELAVAGNGSVGRDPKPKY 478

Query: 211 RGKY 215
           RGKY
Sbjct: 479 RGKY 481

[Close]

BLAST of Lsi03G009000 vs. NCBI nr
Match: XP_038888658.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida])

HSP 1 Score: 373.2 bits (957), Expect = 1.8e-99
Identity = 181/184 (98.37%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 296 MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR
Sbjct: 356 DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTD QLAV GNCN+SKDPKPKY
Sbjct: 416 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDSQLAVGGNCNLSKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. NCBI nr
Match: XP_022932935.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita moschata] >XP_023511692.1 tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-97
Identity = 176/184 (95.65%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           +LAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 
Sbjct: 356 NLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKH 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAA+LKHEEFRENHLKWLEDGNLHSAVFSP+ DPQLAVAGNCN +KDPKPKY
Sbjct: 416 SSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. NCBI nr
Match: KAG7011460.1 (tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.5 bits (937), Expect = 3.7e-97
Identity = 175/184 (95.11%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           +LAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTL+SSSEQILSSRYSRVLLTMVK 
Sbjct: 356 NLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLLSSSEQILSSRYSRVLLTMVKH 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAA+LKHEEFRENHLKWLEDGNLHSAVFSP+ DPQLAVAGNCN +KDPKPKY
Sbjct: 416 SSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. NCBI nr
Match: KAG6571734.1 (tRNA (guanine(10)-N2)-methyltransferase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.5 bits (937), Expect = 3.7e-97
Identity = 175/184 (95.11%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           +LAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTL+SSSEQILSSRYSRVLLTMVK 
Sbjct: 356 NLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLLSSSEQILSSRYSRVLLTMVKH 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAA+LKHEEFRENHLKWLEDGNLHSAVFSP+ DPQLAVAGNCN +KDPKPKY
Sbjct: 416 SSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. NCBI nr
Match: XP_031744574.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus])

HSP 1 Score: 360.5 bits (924), Expect = 1.2e-95
Identity = 175/184 (95.11%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLL
Sbjct: 296 IFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLL 355

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
           DLAAKMLVMGGRLVFFYP+LREEDCVFNQFPEHPCFT ISSSEQILSSRYSRVLLTMVKR
Sbjct: 356 DLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTSISSSEQILSSRYSRVLLTMVKR 415

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
           SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPS +PQL VAGN NVSKDPKPKY
Sbjct: 416 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNPQLGVAGNGNVSKDPKPKY 475

Query: 211 RGKY 215
           RGKY
Sbjct: 476 RGKY 479

[Close]

BLAST of Lsi03G009000 vs. TAIR 10
Match: AT3G26410.1 (methyltransferases;nucleic acid binding )

HSP 1 Score: 281.6 bits (719), Expect = 6.5e-76
Identity = 138/184 (75.00%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 31  MFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 90
           +FDAIICDPPYGVRAGGRKSGGRK+L+GTV PYTVPDDKRTDHIPST AYSLVECVHDLL
Sbjct: 295 IFDAIICDPPYGVRAGGRKSGGRKILRGTVDPYTVPDDKRTDHIPSTGAYSLVECVHDLL 354

Query: 91  DLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR 150
            LAA+MLVM GRLVFF+PVLR+E     +FPEHPCF L++ SEQILSSRYSRVLLTMVK 
Sbjct: 355 HLAARMLVMKGRLVFFFPVLRDETGSEVKFPEHPCFKLVAVSEQILSSRYSRVLLTMVKV 414

Query: 151 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDPQLAVAGNCNVSKDPKPKY 210
             Y+EE+ EAARL H EFRENHLKWLEDGN+HS++F P    Q+    +    KDPKPKY
Sbjct: 415 EPYSEEVEEAARLMHLEFRENHLKWLEDGNIHSSIFKPIDSSQIDT--DSKAFKDPKPKY 474

Query: 211 RGKY 215
           RGKY
Sbjct: 475 RGKY 476

The following BLAST results are available for this feature:

BLAST of Lsi03G009000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54QA6	1.0e-20	43.48	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Dictyostelium discoideum OX=4...	[more]
Q6NS23	7.9e-18	39.10	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Xenopus laevis OX=8355 GN=trm...	[more]
Q6YJI5	1.0e-17	39.47	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Gallus gallus OX=9031 GN=TRMT...	[more]
Q7TNK6	3.9e-17	37.65	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Rattus norvegicus OX=10116 GN...	[more]
Q5R962	5.1e-17	38.65	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT1...	[more]

BLAST of Lsi03G009000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F3J0	1.0e-97	95.65	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita moschata OX=3662 GN...	[more]
A0A0A0K3X0	5.7e-96	95.11	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G229890 PE=4 SV=1	[more]
A0A6J1ICG5	7.5e-96	93.48	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A1S3BG98	3.7e-95	94.57	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1D5Q5	1.7e-92	91.85	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Momordica charantia OX=3673 G...	[more]

BLAST of Lsi03G009000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888658.1	1.8e-99	98.37	tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida]	[more]
XP_022932935.1	2.2e-97	95.65	tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita moschata] >XP_0235116...	[more]
KAG7011460.1	3.7e-97	95.11	tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucurbita argyrosperma sub...	[more]
KAG6571734.1	3.7e-97	95.11	tRNA (guanine(10)-N2)-methyltransferase-like protein, partial [Cucurbita argyros...	[more]
XP_031744574.1	1.2e-95	95.11	tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus]	[more]

BLAST of Lsi03G009000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26410.1	6.5e-76	75.00	methyltransferases;nucleic acid binding	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 11..172 e-value: 9.3E-7 score: 30.5
None	No IPR available	PANTHER	PTHR13370	RNA METHYLASE-RELATED	coord: 31..196
None	No IPR available	PANTHER	PTHR13370:SF19	SUBFAMILY NOT NAMED	coord: 31..196
IPR002052	DNA methylase, N-6 adenine-specific, conserved site	PROSITE	PS00092	N6_MTASE	coord: 35..41
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 26..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G009000.1	Lsi03G009000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0032259	methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity