Lsi03G007560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G007560
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmetacaspase-1
Locationchr03: 10415783 .. 10422604 (+)
RNA-Seq ExpressionLsi03G007560
SyntenyLsi03G007560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACATGCACCTGAAACTTTTATATATTCTCTCTTTCTTCACTCCTTTGGCTTGAGGAAAGGCATAAGAATGGATACCAAAAGTGAATATTGCTGTTGCAAGATCATCAAAAACTACACAAAAATCAATTATGGCTTCAAATGCAAATGCAATGAGTCTTTCAAACCTTCCTCTTCCTCCTCCTCAGGTGTCGGGTCGTTGCGGAGGAGACGAAACACGAGTCGAGATGATGAATCGAGAGGGGAAATGGCTCTCCGGCCATCACCATCTCCAAAGAGAAGCCTCTCGTCTGCCTCCTCGGATGGGCGGCGGACAAAGCGTGCTCTCCTTTGTGGGGTGACTTACAAGAACTGGAAACATAGGCTACATGGGACTGTGAATGATGTTCTGAATATGCAAGATTTGTTGATCAATCATTTTGGATATTACAAACAAAACATTCGTATTCTTACAGGTTATATTTCATCTTTGTGCCTTCAAATTTTTTTTTCCCTTCTTCTTCCTCTAAATTTATGGCTTCTCATTTGATCATTTACTTTGGTGTTAATAAGGACTTGCTTATGTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATATTTTTCTTTCTTTCTTGTTTTTGGGGTTTTGAAAGAAGAAATTTTTTAGGAATTTAAAAAAAAGAAAATGTTTGATGAAATCATTTAATTGTTCTTTCATTTTAATAGAAAAATGAAACAAAAATGTATTATTCCAATTCCAATGACAACTGTTTTGGTCCCTTAGTTCTGGAAATTTCTATGGGTTCCTTCCTTATCATGAAACTTAAAGTAACTTTAACCCCAAAATAAAAAATTAAAAAAACAAAAAAACAAAAAAAAAAAGTGTGTGGGAATTGACTTGTGTTTTAAAGTTCCATAATTTGAGTTATTTGGTACTGAAAATTATGTTTCCAGTGATAATTATTGAATTGAAATTTTATGGGAACTTTTTTTTTAATAATATATGAGGAGAGAAAATCGAATCTCAGAACTTGAGATTGATAGTACAAGTTTTATATCATTTGAGTTATGCTCGTTTTGGTCATTTTTGTGAGAACTTAGGGTTGTTTGTTTAGTGGGAATCCAATACTCCTCAATGGAGTGGTCATCTAGATTACTCTGTTTGTTTTGTGGTAATGTGAGATACTCTAACATTTGGAGTTTCTCGGGCATACTAAGATTCCTTACGAAGGGCATCTCAAGAGGTTTAGATACCTTCTTGAAACATGGACTTTTCAAATTCTCATGTTGAGGGAACCTCCTTTTTATTTTATTTTTTTTATCCTTTTTTATATATACACACATGTAACTAAAATAAATATCGCATCCCTTTTATTCTTTTTTAAGAAAGATGTTCAAATTTAAGTTTAAATTAATATTTATTTTATTAAAATAATCTTAAATTAATATATATTATTTTGTTGAGAAAAATAGTCTATAAAAACATTAAAAACTATATTAATTTAAAAATATATTCAAATTAATAAAAAAAATATATGTCATATAAGTTAAAATTATATTAAATTAATTATAAATTAATGAATAGCTGTGAGTGCATTTCTTGAAAGATTATATATAAATCTATGACATTTCTAACTAAAATTCTACGAAATAAATACAGTTATCCAAGTATTTCTATGTATCTACAAAAAATAGTCCTACAAAACAAATATGGTTATTTAAAGATGTCGACAATTTGACATTTAGAGTATTTAAAGATTCTAGTTTATCTATATTTCCAAAAAACAAACAGCACCTTAGAGACCAGAATAAAATATTTCAAACCTATAATTAATTATTTGAAGGAAAAAAAAGCAAATTATATACTTAAATTCTGAGTTGTTTCACTTATAAAATTTTAAACAAAAATTGTATCAATTAAAACTCTAAGCTTTTACAAATAATTAAATTAATTTAGATTTTAAACTTTCACAAATAAATCAATTTAAGACCCTTCGTCAAAAACAAAAAATCCTAAAATCAACTCAAATTGACCATCTAATAAAATTATTTTTCTCCCAAAATCCAACCGAATTTGTCAAAAACACAATTAATGGCATAATTTTTTCATATAAATGGAGGATCAAAATTCACTCATATTAATGAAATATTATTATTTTCCATTTGATAACCTTTCCTTCTAATATTACATTAATCTATGTATTTAAAAAAAAGTTTTACAAAAATTATGCAATAATGGGTTTTATAGCATAATATTAAGGTGAAGTCTAAATTACTATAAAAAGTTTATAAATTTAATTGATATAATTAATTAATAGTTAGGAGTTATAAGAGTTAAATTTGTCAAGTTTAAAGAATATAAATTAATTTTTTATTCTTTAATAAAAATGAGTATGATATAAATTTTTTTAAGATGGAATCTTTAATGTTGTAGTTGATAATACAACGTAGCTAAGCTAATTTTAACATTATGTGAGAACTTTTTTTAGAGAGCATCCCCAAGTGAAACGTGCTTTTTATAAGCAATTTTGGAAGAGAACTTTCTTTTGAAGTATCTTTATAAAACTTTCAACATTACTTTTGACTAATTAGTAAAATATAGAAAAGTATACTTTTAATGGTTAAGAATACTTTCCTCTCCTTCCCAAAAGTAATCTAAACCCCATTTTAAATAGAAAAAAAATTGGAGAATTTTTAAAAATAGAAAAATAAAGAAAACTATTTACACATAATAACAAAATTTTTAGATAGTTGTGATAGACGTTGATAGAAGTCTATCAGGGTCTATCAGTGATAGAATGATAGAAGCATATCACTGATAGATGCTGATAGAAGTCTATCAATGTCTATCAATATTTTTTTTGCTATTTTCTGTAAATAGTTTGACATTTTTTCTATCACTAAAAAATTTCCAAAAAAATTATATATATCCAAAATCAAAACTAAAGTATGGATGCTTCTATTCATTAGTGTCGTGTGATGTAAGTATCTTTTTAAAATTAAAGTAATAATGAGATTCATTATCATTATATTTGAAAATATATATTTATAATAAAGGGAGTATAGTATTACTCACGTAAATCAACTTGAAATTTATCTATTTTAATAAATAGAATAATTGTATAAATTAGAGAAAGTTTTAAAAATAGAAAAATAAGATAAACTATTTACACAAAATAGTAAAATTTTTAAATAGTTGTGATAGATGCTGATAGAAGTCTATCAGGATCTATCAGCAATACAAATGATAGAAGTCTATCACTGATAGATGGTGATAGAAGTCTATCACTGATAGATGGTGATAGAAGTCTATCAATGTCTATCAGTATTTTTTTTGCTATTTTATGTAAATAGTTTGACATTTTTTTTATTTGTAAAAATTTCCCTATAAATTATTCACTTATTTTTAGTTTTAAAGGGTATATCTGTCCTCTCTACATTTACTCTTGTTTCATAACAGTCCTAATTAATACTATCAAATTTATATTATAGTCCCTAAATTGTAATCTATTTTAGTTCTTTATTGAATATTCACTAATTATTATTATGTTGCATTCACTAATTATTCCTTGTGTTGCATTCACTAGGTTTAAAGGATTAGTCCTTGTGTTTGCATTCACTAATTATTCAAGAAAAATTTGTGGCTATATCATGTGAACTTGAACTTGAATAATAGTCACATGTATTAATATACAAATCTACTATTTACTCTAAAAGTGGACTGGATAACATAAGTTTATGTAGAAAGTAGCATGGTAAATATAGATTTTTATAAGAAATTCTAGCACTCCCAAAAACATAGGAGGATTGAAATAAAGGTTATCTAACACACCGTGCAAACTTGTTACAACAATTGTGTAAGGACAATCAGTAAAGCACTGATAAAAACATTTAATGATAGAACACGAGGTTTGGTAACCTAGTTTGGTGCAATACCACCTACGTCTAGGGCAGTGTGCCTAGGAAATATAAATTCACTAATATAAAGTTAAGCGATTACAATATTGTACTTAGCTGTAAATAAAGGGCAAATATAATGACACACGAATAGCCCAACTTAACGTGCATGATCACTTAGGCTCCCTTAAGTGTGGGACTCCCACTCAATAAAACTTAAGGCTCCCCCTAAGTGTGAGACTAGCTCCCTCTCACCAAAAACTTTTATTTTTTCACCTTCAAATAAAGATCACTCCACTTGATTAACTTAGGCTCCTCCTAAGTTTGAGAATCTCTTCTCAAGTAACGAATAAAGCTTCAAGATGATCTAGAGAAAAACTTCAAGATCTATAATGCTTCTTGCTTCGCAATCACTAAAATTGACACACGTTTTTGTTGTTTGGCTAACACACGAGACAAGAACAAACAATCAAAGCGGTAACCCTGAATCATAAAATATCGATGTCAATATCCTAACTGTTTTTCCATTCAACATTATTGTTTTTTTTTTTTTTTTTACTATATCTCATTTAATATTCTATGATAGAATAAAAATATGAAAATGTCAATGTAAATTTAATACTCTCACTAACCCACCAATTAACACAGAGGACGAAGCAATGCCAGAGCGACTACCAACAAAGAAGAACATCCAAAGCAGTCTAAAATGGCTAGTTGAAGGTTGCACCGGCGGTGAAAGCCTCGTGTTCTACTTCTCCGGCCATGGATTACGACAGCCAGATTTCGAGATGGACGAGCTCGATGGCTACGACGAGACCATATGTCCAGTTGACTTCATGGAGGAAGGGATGATAAGCGATAATGAGATCAATGCCACCATTGTTTCCCCTCTAAAGAACGGCGTCATCCTCCACGCCATCGTCGACGCCTGCCATAGCGGAACTATTCTCGATCTCGCCTATGTTTACGACCGCAACAGGTTTCAATTGTACTCTTTTTTTTCCTTTCTTTTTTTTATTTTGTTATTTTGATTTTGATTTTGGATTGGGATTTTTTTTTTTTCTTTTAATGATGGAAGGGATGAATGGCTGGATAATAGACCGCCGTCGGGAGCGAGGAAGGCGACTAGTGGTGGATTGGCAATTTCTTTGAGTGCCTGTGAAGATGATCAATTTGCTGCTGATACTTCTGTAAGACTACACATATCCTTCATCTTCTAAGTTCTATCTATAATTTTTAGTTGTGCATTTCTTTTCTCTCTTTTTTTGTTTTTCATTTTGTCAAATTATAAGTTTGGTTTCAAAACTTTTATGTCCGCGTTTCATAGGTTCCGAAGCTTTAAAAGTATCTAATAGGTTCTCAATTTTTAATTTTGTGTCTAATTAGTCTTTGATATATTCGATCTTTTTAAGAAAACTAACGAAACTAATTGATCGATAAATTTTTAATTTTGTGTCGAGTTACTATTGAGCTTTAGATCACTTGTTGATAAATAAATAGACAATGGAACCTCTACCTATATCTACAATGGTATGATTTGTCCTTCTAAAAAGCTTCATGTCTCTCTCACTTTTATATATATATATATATAGCCAATGTGAAACTTTGATTGAATTTCAAACAATCCTCTCCTAGAACAAAGAATCATCAAGCCTACCCCTCAAATAGTCATTCTTTCAATCTAACTCTTTTTCTCTAGAGCACATTGTCTATTCGATAGAGCTCAACCACAGATCATACCATTTTTTAGGGACAATGGCCTTCCAAATAACTTCATAAATAGGGTATGATATTGTCTAAAATATCTCATACCAATGATGATAGTTGTCCTAACTTATATAATCATGATCTTTCCCTTATCTGATTAATGTGGGATTGTAGTCGCATTCCCATCATATAGTCATTCTAAATCAGCTTTAAGCTACCTAATTAGTAAATACTATACTTTCTAAAGTAACTTATCTTCTAGACACTTCTAAGAAATTGGCAAAGTTTTCAATGACGGATCTTATTTTAAATAGCTTTGGTTTCATGGTTAGATTTCTACCAAAAGTATCATAGAGTAAGAAAAACATTTTACGAAAGCTAAATATGAAAATGGTTATAATTAAAGTTGAGAGATATCCAAATAGAACGAAATGAGACTTGAACTCTATAACGGTAAGACAAAAAAGAAAAAGAAAGAGAGATCTTGATGGTATAATATTAATAGATATGGAACTAAGTTTTAGAAGTTGATAATGGGAATGATTTATATATAATGCAGATATTAACAGGGAAGTCAATGAATGGAGCAATGACATTCATTCTCATTCATTTGGTTAAGACTTTTGGAAACTTGACGTACGGACGCCTGCTTGAATACATGCATGATGCTGTTCAAAGGGCCAACAAACAAGGCTGTTTCTCATGTTCTTTTTTAAGAAAATTGCTTCGTTATAAACAGATTCAGGTCATTTACTCATTACTCTAAGTGAATAGTCAATTTTTAACTTTAAACTTGAATAAGTTATAGTGGTTAATATATATTCAAAATTGAATTTAAAATTGCTCATGAACAACGAAATAAAGAAGAGAGCTTGAGTATAAATTAAATTCAATATGGATTAAGAACAAACGTTTGGAACTTAACAATGAAATGCTTAAATACAACTTCCACTCACCCACTTAAGTTTCGTAGGAAAAATATTAACTTTCCTTTTGATATTTTGATGTTTTGCAGGAACCACAACTCTCATCGTCAGAGGTGTTTGATGTACACAAGAAAATATTTACATTGTAATTGCATTTCTTTTGGTTTTTCCTACTAAATCTGAATGTACATTCTAAAACAATAGTTTGAAATTGTTTAGAGAGAAAAGTCAACTCTCTCAAATATCAAAATATTTACATTAATAGTAATTGAATTTCTTTTGGTTTT

mRNA sequence

AAACATGCACCTGAAACTTTTATATATTCTCTCTTTCTTCACTCCTTTGGCTTGAGGAAAGGCATAAGAATGGATACCAAAAGTGAATATTGCTGTTGCAAGATCATCAAAAACTACACAAAAATCAATTATGGCTTCAAATGCAAATGCAATGAGTCTTTCAAACCTTCCTCTTCCTCCTCCTCAGGTGTCGGGTCGTTGCGGAGGAGACGAAACACGAGTCGAGATGATGAATCGAGAGGGGAAATGGCTCTCCGGCCATCACCATCTCCAAAGAGAAGCCTCTCGTCTGCCTCCTCGGATGGGCGGCGGACAAAGCGTGCTCTCCTTTGTGGGGTGACTTACAAGAACTGGAAACATAGGCTACATGGGACTGTGAATGATGTTCTGAATATGCAAGATTTGTTGATCAATCATTTTGGATATTACAAACAAAACATTCGTATTCTTACAGAGGACGAAGCAATGCCAGAGCGACTACCAACAAAGAAGAACATCCAAAGCAGTCTAAAATGGCTAGTTGAAGGTTGCACCGGCGGTGAAAGCCTCGTGTTCTACTTCTCCGGCCATGGATTACGACAGCCAGATTTCGAGATGGACGAGCTCGATGGCTACGACGAGACCATATGTCCAGTTGACTTCATGGAGGAAGGGATGATAAGCGATAATGAGATCAATGCCACCATTGTTTCCCCTCTAAAGAACGGCGTCATCCTCCACGCCATCGTCGACGCCTGCCATAGCGGAACTATTCTCGATCTCGCCTATGTTTACGACCGCAACAGACCGCCGTCGGGAGCGAGGAAGGCGACTAGTGGTGGATTGGCAATTTCTTTGAGTGCCTGTGAAGATGATCAATTTGCTGCTGATACTTCTGAACCACAACTCTCATCGTCAGAGGTGTTTGATGTACACAAGAAAATATTTACATTGTAATTGCATTTCTTTTGGTTTTTCCTACTAAATCTGAATGTACATTCTAAAACAATAGTTTGAAATTGTTTAGAGAGAAAAGTCAACTCTCTCAAATATCAAAATATTTACATTAATAGTAATTGAATTTCTTTTGGTTTT

Coding sequence (CDS)

ATGGATACCAAAAGTGAATATTGCTGTTGCAAGATCATCAAAAACTACACAAAAATCAATTATGGCTTCAAATGCAAATGCAATGAGTCTTTCAAACCTTCCTCTTCCTCCTCCTCAGGTGTCGGGTCGTTGCGGAGGAGACGAAACACGAGTCGAGATGATGAATCGAGAGGGGAAATGGCTCTCCGGCCATCACCATCTCCAAAGAGAAGCCTCTCGTCTGCCTCCTCGGATGGGCGGCGGACAAAGCGTGCTCTCCTTTGTGGGGTGACTTACAAGAACTGGAAACATAGGCTACATGGGACTGTGAATGATGTTCTGAATATGCAAGATTTGTTGATCAATCATTTTGGATATTACAAACAAAACATTCGTATTCTTACAGAGGACGAAGCAATGCCAGAGCGACTACCAACAAAGAAGAACATCCAAAGCAGTCTAAAATGGCTAGTTGAAGGTTGCACCGGCGGTGAAAGCCTCGTGTTCTACTTCTCCGGCCATGGATTACGACAGCCAGATTTCGAGATGGACGAGCTCGATGGCTACGACGAGACCATATGTCCAGTTGACTTCATGGAGGAAGGGATGATAAGCGATAATGAGATCAATGCCACCATTGTTTCCCCTCTAAAGAACGGCGTCATCCTCCACGCCATCGTCGACGCCTGCCATAGCGGAACTATTCTCGATCTCGCCTATGTTTACGACCGCAACAGACCGCCGTCGGGAGCGAGGAAGGCGACTAGTGGTGGATTGGCAATTTCTTTGAGTGCCTGTGAAGATGATCAATTTGCTGCTGATACTTCTGAACCACAACTCTCATCGTCAGAGGTGTTTGATGTACACAAGAAAATATTTACATTGTAA

Protein sequence

MDTKSEYCCCKIIKNYTKINYGFKCKCNESFKPSSSSSSGVGSLRRRRNTSRDDESRGEMALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYVYDRNRPPSGARKATSGGLAISLSACEDDQFAADTSEPQLSSSEVFDVHKKIFTL
Homology
BLAST of Lsi03G007560 vs. ExPASy Swiss-Prot
Match: Q7XJE6 (Metacaspase-1 OS=Arabidopsis thaliana OX=3702 GN=AMC1 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-58
Identity = 108/197 (54.82%), Postives = 147/197 (74.62%), Query Frame = 0

Query: 83  KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKN 142
           KRA++CG++Y+  +H L G +ND   M+ LLIN F +   +I +LTE+E  P R+PTK+N
Sbjct: 80  KRAVICGISYRFSRHELKGCINDAKCMRHLLINKFKFSPDSILMLTEEETDPYRIPTKQN 139

Query: 143 IQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEI 202
           ++ +L WLV+GCT G+SLVF++SGHG RQ ++  DE+DGYDET+CP+DF  +GMI D+EI
Sbjct: 140 MRMALYWLVQGCTAGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLDFETQGMIVDDEI 199

Query: 203 NATIVSPLKNGVILHAIVDACHSGTILDL----------AYVYDRNRPPSGARKATSGGL 262
           NATIV PL +GV LH+I+DACHSGT+LDL           YV++ +RP SG  K T+GG 
Sbjct: 200 NATIVRPLPHGVKLHSIIDACHSGTVLDLPFLCRMNRAGQYVWEDHRPRSGLWKGTAGGE 259

Query: 263 AISLSACEDDQFAADTS 270
           AIS+S C+DDQ +ADTS
Sbjct: 260 AISISGCDDDQTSADTS 276

BLAST of Lsi03G007560 vs. ExPASy Swiss-Prot
Match: Q7XJE5 (Metacaspase-2 OS=Arabidopsis thaliana OX=3702 GN=AMC2 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 6.7e-50
Identity = 105/204 (51.47%), Postives = 135/204 (66.18%), Query Frame = 0

Query: 83  KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKN 142
           KRA++ GV+YKN K  L G +ND   M+ +L+  F + +  I +LTE+EA P R PTK N
Sbjct: 116 KRAVIVGVSYKNTKDELKGCINDANCMKFMLMKRFQFPESCILMLTEEEADPMRWPTKNN 175

Query: 143 IQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEI 202
           I  ++ WLV  C  G+SLVF+FSGHG  Q D   DE+DG+DET+ PVD    G+I D+EI
Sbjct: 176 ITMAMHWLVLSCKPGDSLVFHFSGHGNNQMDDNGDEVDGFDETLLPVDHRTSGVIVDDEI 235

Query: 203 NATIVSPLKNGVILHAIVDACHSGTILDLAYV----------YDRNRPPSGARKATSGGL 262
           NATIV PL  GV LHAIVDACHSGT++DL Y+          ++ +RP +G  K TSGG 
Sbjct: 236 NATIVRPLPYGVKLHAIVDACHSGTVMDLPYLCRMDRLGNYEWEDHRPKTGMWKGTSGGE 295

Query: 263 AISLSACEDDQFAADTSEPQLSSS 277
             S + C+DDQ +ADT  PQLS S
Sbjct: 296 VFSFTGCDDDQTSADT--PQLSGS 317

BLAST of Lsi03G007560 vs. ExPASy Swiss-Prot
Match: Q9FMG1 (Metacaspase-3 OS=Arabidopsis thaliana OX=3702 GN=AMC3 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.0e-47
Identity = 100/238 (42.02%), Postives = 142/238 (59.66%), Query Frame = 0

Query: 40  GVGSLRRRRNTSRDDESRGEMALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRL 99
           G   L R+      ++ + +M  +P P     L S        KRA+LCGV YK   + L
Sbjct: 51  GFQQLLRQHQPQHHEQQQQQMMAQPPPRLLEPLPSPFG----KKRAVLCGVNYKGKSYSL 110

Query: 100 HGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGES 159
            G ++D  +M+ LL+   G+   +I +LTEDEA P+R+PTK+NI+ +++WLVEG    +S
Sbjct: 111 KGCISDAKSMRSLLVQQMGFPIDSILMLTEDEASPQRIPTKRNIRKAMRWLVEGNRARDS 170

Query: 160 LVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAI 219
           LVF+FSGHG +Q D+  DE+DG DE +CP+D   EG I D+EIN  +V PL +G  LHA+
Sbjct: 171 LVFHFSGHGSQQNDYNGDEIDGQDEALCPLDHETEGKIIDDEINRILVRPLVHGAKLHAV 230

Query: 220 VDACHSGTILDLAYVYDRNRPPS---------GARKATSGGLAISLSACEDDQFAADT 269
           +DAC+SGT+LDL ++    R  S          A K T GG A   SAC+DD+ +  T
Sbjct: 231 IDACNSGTVLDLPFICRMERNGSYEWEDHRSVRAYKGTDGGAAFCFSACDDDESSGYT 284

BLAST of Lsi03G007560 vs. ExPASy Swiss-Prot
Match: Q2UN81 (Metacaspase-1A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=casA PE=3 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 9.0e-39
Identity = 80/176 (45.45%), Postives = 116/176 (65.91%), Query Frame = 0

Query: 82  TKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKK 141
           T++ALL G+ Y N K +L G +NDV NM   L  +FGY ++N+ +LT+D+  P+  PTK 
Sbjct: 105 TRKALLIGINYFNQKGQLRGCINDVKNMSTYLHENFGYPRENMVLLTDDQQNPKSQPTKA 164

Query: 142 NIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNE 201
           NI  ++ WLV+     +SL F++SGHG + PD + DE DGYDE I PVDF + G I D+E
Sbjct: 165 NILRAMHWLVKDAKPNDSLFFHYSGHGGQTPDLDGDEDDGYDEVIYPVDFRQAGHIVDDE 224

Query: 202 INATIVSPLKNGVILHAIVDACHSGTILDLAYVYDRN---RPPSGARKATSGGLAI 255
           ++  +V+PL+ GV L AI D+CHSG+ LDL Y+Y      + P+ A++A  G L +
Sbjct: 225 MHRIMVNPLQPGVRLTAIFDSCHSGSALDLPYIYSTQGILKEPNLAKEAGQGLLGV 280

BLAST of Lsi03G007560 vs. ExPASy Swiss-Prot
Match: A2RB75 (Metacaspase-1A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=casA PE=3 SV=2)

HSP 1 Score: 156.4 bits (394), Expect = 4.9e-37
Identity = 78/175 (44.57%), Postives = 112/175 (64.00%), Query Frame = 0

Query: 83  KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKN 142
           ++ALL G+ Y N K +L G +NDV NM   L  +FGY ++++ +LT+D+  P   PTK N
Sbjct: 116 RKALLIGINYFNQKGQLRGCINDVKNMSTYLNQNFGYAREDMVVLTDDQQNPMSQPTKAN 175

Query: 143 IQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEI 202
           I  ++ WLV+     +SL F++SGHG + PD + DE DGYDE I PVDF   G I D+E+
Sbjct: 176 ILRAMHWLVKDAQPNDSLFFHYSGHGGQTPDLDGDEDDGYDEVIYPVDFRAAGHIVDDEM 235

Query: 203 NATIVSPLKNGVILHAIVDACHSGTILDLAYVYDRN---RPPSGARKATSGGLAI 255
           +  +V PL+ GV L AI D+CHSG+ LDL Y+Y      + P+ A++A  G L +
Sbjct: 236 HRIMVKPLQPGVRLTAIFDSCHSGSALDLPYIYSTQGILKEPNLAKEAGQGLLGV 290

BLAST of Lsi03G007560 vs. ExPASy TrEMBL
Match: A0A0A0K220 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G062910 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.4e-121
Identity = 252/363 (69.42%), Postives = 263/363 (72.45%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKC-NESFKP-SSSSSSGVG-SLRRRRNTSRDDESR 60
           M TKSEYCCCKI KN TKIN  FKCKC N SFKP SSSSSSG G SLRRRR  S+DDES+
Sbjct: 1   MYTKSEYCCCKINKNPTKINGSFKCKCNNHSFKPSSSSSSSGAGVSLRRRRYASQDDESK 60

Query: 61  GEMALRPS-PSPKRSLSS-ASSDGRRT-KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLI 120
            E  L PS   P R+LSS ASSDGRRT KRALLCGVTYKNWKHRL GTVNDV NMQDLLI
Sbjct: 61  PEKVLWPSVQPPMRTLSSTASSDGRRTIKRALLCGVTYKNWKHRLQGTVNDVRNMQDLLI 120

Query: 121 NHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDF 180
           NHFGY KQNIRILTEDE  PE++PTKKNIQ+ LKWLVEGCTGG++LVFYFSGHGLRQPDF
Sbjct: 121 NHFGYSKQNIRILTEDETKPEQMPTKKNIQNGLKWLVEGCTGGDNLVFYFSGHGLRQPDF 180

Query: 181 EMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYV 240
           +MDELDGYDETICPVDFMEEGMI+DNEINATIVSPLKNGV LHAIVDACHSGTILDLAYV
Sbjct: 181 DMDELDGYDETICPVDFMEEGMITDNEINATIVSPLKNGVTLHAIVDACHSGTILDLAYV 240

Query: 241 YDR-------NRPPSGARKATSGGLAISLSACEDDQFAADTS------------------ 289
           YDR       NRPPSGARK TSGGLAIS+SAC DDQFAADTS                  
Sbjct: 241 YDRDRDEWLDNRPPSGARKETSGGLAISMSACGDDQFAADTSILTGKTMNGAMTFILIHL 300

BLAST of Lsi03G007560 vs. ExPASy TrEMBL
Match: A0A1S3BZX6 (metacaspase-1 OS=Cucumis melo OX=3656 GN=LOC103495419 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.7e-120
Identity = 251/367 (68.39%), Postives = 265/367 (72.21%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFK-CKC--NESFKP-SSSSSSGVG-SLRRRRNTSRDDE 60
           M +KSEYCCCKI KN+TKIN GFK CKC  N SFKP SSSSSSG G +LRRRR  S+DDE
Sbjct: 1   MYSKSEYCCCKINKNHTKINNGFKYCKCNNNHSFKPSSSSSSSGAGVALRRRRYASQDDE 60

Query: 61  SR--GEMALRPS--PSPKRSLSSASSDGRRT-KRALLCGVTYKNWKHRLHGTVNDVLNMQ 120
           SR   +  L PS  P P R+LSS SSDGRRT KRALLCGVTYKNWKHRL GTVNDV NMQ
Sbjct: 61  SRPDHQTVLWPSSVPPPTRTLSSTSSDGRRTIKRALLCGVTYKNWKHRLQGTVNDVRNMQ 120

Query: 121 DLLINHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLR 180
           DLLIN+FGY KQNIRILTEDE  PE++PTKKNIQ+ LKWLVEGCTGGE+LVFYFSGHGLR
Sbjct: 121 DLLINYFGYSKQNIRILTEDETKPEQIPTKKNIQNGLKWLVEGCTGGENLVFYFSGHGLR 180

Query: 181 QPDFEMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILD 240
           QPDF+MDELDGYDETICPVDFMEEGMI+DNEINATIVSPLKNGV LHAIVDACHSGTILD
Sbjct: 181 QPDFDMDELDGYDETICPVDFMEEGMITDNEINATIVSPLKNGVTLHAIVDACHSGTILD 240

Query: 241 LAYVYDR-------NRPPSGARKATSGGLAISLSACEDDQFAADTS-------------- 289
           LAYVYDR       NRPPSGARK TSGGLAIS+SAC DDQFAADTS              
Sbjct: 241 LAYVYDRNRNEWLDNRPPSGARKETSGGLAISMSACGDDQFAADTSILTGKTMNGAMTFI 300

BLAST of Lsi03G007560 vs. ExPASy TrEMBL
Match: A0A6J1EJD3 (metacaspase-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434681 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.1e-116
Identity = 240/357 (67.23%), Postives = 254/357 (71.15%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKCNESFKPSSSSSSGVGSLRRRRNTSRDDESRGEM 60
           MDTKSE  CCKI  N+TK+   FKCKC + FKPSSSSSS VG+L    +TSRDDE   EM
Sbjct: 1   MDTKSECWCCKIKNNHTKLT-PFKCKCIK-FKPSSSSSSDVGAL----HTSRDDELGAEM 60

Query: 61  ALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYY 120
            LRP PSP +SLSS++SD    KRALLCGV+YKNWKHRLHGTVNDVLNMQDLLINHF Y 
Sbjct: 61  ELRPPPSPMKSLSSSASDTGLRKRALLCGVSYKNWKHRLHGTVNDVLNMQDLLINHFSYL 120

Query: 121 KQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELD 180
           KQNIRILTEDEA PER+PTKKNIQSSLKWLVE C GGESLVFYFSGHGLRQPDF MDELD
Sbjct: 121 KQNIRILTEDEANPERIPTKKNIQSSLKWLVEDCYGGESLVFYFSGHGLRQPDFAMDELD 180

Query: 181 GYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYVYDR--- 240
           GYDETICPVDF+EEGMISDNEINATIVSPL++GV LHAIVDACHSGTILDLAYVYDR   
Sbjct: 181 GYDETICPVDFLEEGMISDNEINATIVSPLRSGVNLHAIVDACHSGTILDLAYVYDRTRD 240

Query: 241 ----NRPPSGARKATSGGLAISLSACEDDQFAADTS------------------------ 289
               NRPPSGA KATSGGLAISLSAC DDQFAADTS                        
Sbjct: 241 EWIDNRPPSGASKATSGGLAISLSACGDDQFAADTSILTGKSMNGAMTFILIDLIKTYGN 300

BLAST of Lsi03G007560 vs. ExPASy TrEMBL
Match: A0A6J1JNG9 (metacaspase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111486063 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.9e-116
Identity = 240/358 (67.04%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKCNESFKPSSSSSSG-VGSLRRRRNTSRDDESRGE 60
           MDTKSE CCCKI  N+TK+   FKCKC + FKPSSSSSS   G+L    +TSRDDES  E
Sbjct: 1   MDTKSECCCCKIKNNHTKLT-PFKCKCIK-FKPSSSSSSSDAGAL----HTSRDDESGTE 60

Query: 61  MALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGY 120
           M LRP PSP +SLSS++SD    KRALLCGV+YKNWKHRLHGTVNDVLNMQDLLINHF Y
Sbjct: 61  MGLRPPPSPMKSLSSSASDAGLRKRALLCGVSYKNWKHRLHGTVNDVLNMQDLLINHFSY 120

Query: 121 YKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDEL 180
            KQNIRILTE+EA PER+PTKKNIQSSLKWLVE C GGESLVFYFSGHGLRQPDF MDEL
Sbjct: 121 LKQNIRILTENEANPERIPTKKNIQSSLKWLVEDCYGGESLVFYFSGHGLRQPDFAMDEL 180

Query: 181 DGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYVYDR-- 240
           DGYDETICPVDF+EEGMISDNEINATIVSPL++GV LHAIVDACHSGTILDLAYVYDR  
Sbjct: 181 DGYDETICPVDFLEEGMISDNEINATIVSPLRSGVNLHAIVDACHSGTILDLAYVYDRTR 240

Query: 241 -----NRPPSGARKATSGGLAISLSACEDDQFAADTS----------------------- 289
                NRPPSGA KATSGGLAISLSAC DDQFAADTS                       
Sbjct: 241 DEWIDNRPPSGASKATSGGLAISLSACGDDQFAADTSILTGKSMNGAMTFILINMIKSFG 300

BLAST of Lsi03G007560 vs. ExPASy TrEMBL
Match: A0A6J1EME3 (metacaspase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434681 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.8e-115
Identity = 223/276 (80.80%), Postives = 236/276 (85.51%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKCNESFKPSSSSSSGVGSLRRRRNTSRDDESRGEM 60
           MDTKSE  CCKI  N+TK+   FKCKC + FKPSSSSSS VG+L    +TSRDDE   EM
Sbjct: 1   MDTKSECWCCKIKNNHTKLT-PFKCKCIK-FKPSSSSSSDVGAL----HTSRDDELGAEM 60

Query: 61  ALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYY 120
            LRP PSP +SLSS++SD    KRALLCGV+YKNWKHRLHGTVNDVLNMQDLLINHF Y 
Sbjct: 61  ELRPPPSPMKSLSSSASDTGLRKRALLCGVSYKNWKHRLHGTVNDVLNMQDLLINHFSYL 120

Query: 121 KQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELD 180
           KQNIRILTEDEA PER+PTKKNIQSSLKWLVE C GGESLVFYFSGHGLRQPDF MDELD
Sbjct: 121 KQNIRILTEDEANPERIPTKKNIQSSLKWLVEDCYGGESLVFYFSGHGLRQPDFAMDELD 180

Query: 181 GYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYVYDR--- 240
           GYDETICPVDF+EEGMISDNEINATIVSPL++GV LHAIVDACHSGTILDLAYVYDR   
Sbjct: 181 GYDETICPVDFLEEGMISDNEINATIVSPLRSGVNLHAIVDACHSGTILDLAYVYDRTRD 240

Query: 241 ----NRPPSGARKATSGGLAISLSACEDDQFAADTS 270
               NRPPSGA KATSGGLAISLSAC DDQFAADTS
Sbjct: 241 EWIDNRPPSGASKATSGGLAISLSACGDDQFAADTS 270

BLAST of Lsi03G007560 vs. NCBI nr
Match: XP_038888839.1 (metacaspase-1-like [Benincasa hispida])

HSP 1 Score: 503.8 bits (1296), Expect = 9.6e-139
Identity = 272/358 (75.98%), Postives = 277/358 (77.37%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKCNESFKPSSSSSSGVGSLRRRRNTSRDDESRGE- 60
           MDTKSEYCCCKIIKN+TKIN GFKCKCNESFKPSSSSSSG G+ RRR N+SRDDESR E 
Sbjct: 1   MDTKSEYCCCKIIKNHTKIN-GFKCKCNESFKPSSSSSSGAGAFRRRLNSSRDDESRQEI 60

Query: 61  MALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGY 120
           MAL PSPSPKR+LSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGY
Sbjct: 61  MALWPSPSPKRNLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGY 120

Query: 121 YKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDEL 180
            KQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCT GESLVFYFSGHGLRQPDFEMDEL
Sbjct: 121 SKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTDGESLVFYFSGHGLRQPDFEMDEL 180

Query: 181 DGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYVYD--- 240
           DGYDETICPVDFMEEGMISDNEINATIVSPLK GV+LHAIVDACHSGTILDLAYVYD   
Sbjct: 181 DGYDETICPVDFMEEGMISDNEINATIVSPLKKGVVLHAIVDACHSGTILDLAYVYDLNR 240

Query: 241 ----RNRPPSGARKATSGGLAISLSACEDDQFAADTS----------------------- 289
                NRPPSGARKATSGGLAISLSAC DDQFAADTS                       
Sbjct: 241 DDWLDNRPPSGARKATSGGLAISLSACGDDQFAADTSILTGKSMNGAMTFIMIHLVKTFA 300

BLAST of Lsi03G007560 vs. NCBI nr
Match: XP_004136878.1 (metacaspase-1 [Cucumis sativus])

HSP 1 Score: 444.5 bits (1142), Expect = 6.9e-121
Identity = 252/363 (69.42%), Postives = 263/363 (72.45%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKC-NESFKP-SSSSSSGVG-SLRRRRNTSRDDESR 60
           M TKSEYCCCKI KN TKIN  FKCKC N SFKP SSSSSSG G SLRRRR  S+DDES+
Sbjct: 1   MYTKSEYCCCKINKNPTKINGSFKCKCNNHSFKPSSSSSSSGAGVSLRRRRYASQDDESK 60

Query: 61  GEMALRPS-PSPKRSLSS-ASSDGRRT-KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLI 120
            E  L PS   P R+LSS ASSDGRRT KRALLCGVTYKNWKHRL GTVNDV NMQDLLI
Sbjct: 61  PEKVLWPSVQPPMRTLSSTASSDGRRTIKRALLCGVTYKNWKHRLQGTVNDVRNMQDLLI 120

Query: 121 NHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDF 180
           NHFGY KQNIRILTEDE  PE++PTKKNIQ+ LKWLVEGCTGG++LVFYFSGHGLRQPDF
Sbjct: 121 NHFGYSKQNIRILTEDETKPEQMPTKKNIQNGLKWLVEGCTGGDNLVFYFSGHGLRQPDF 180

Query: 181 EMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYV 240
           +MDELDGYDETICPVDFMEEGMI+DNEINATIVSPLKNGV LHAIVDACHSGTILDLAYV
Sbjct: 181 DMDELDGYDETICPVDFMEEGMITDNEINATIVSPLKNGVTLHAIVDACHSGTILDLAYV 240

Query: 241 YDR-------NRPPSGARKATSGGLAISLSACEDDQFAADTS------------------ 289
           YDR       NRPPSGARK TSGGLAIS+SAC DDQFAADTS                  
Sbjct: 241 YDRDRDEWLDNRPPSGARKETSGGLAISMSACGDDQFAADTSILTGKTMNGAMTFILIHL 300

BLAST of Lsi03G007560 vs. NCBI nr
Match: KGN43776.2 (hypothetical protein Csa_017238 [Cucumis sativus])

HSP 1 Score: 444.5 bits (1142), Expect = 6.9e-121
Identity = 252/363 (69.42%), Postives = 263/363 (72.45%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKC-NESFKP-SSSSSSGVG-SLRRRRNTSRDDESR 60
           M TKSEYCCCKI KN TKIN  FKCKC N SFKP SSSSSSG G SLRRRR  S+DDES+
Sbjct: 13  MYTKSEYCCCKINKNPTKINGSFKCKCNNHSFKPSSSSSSSGAGVSLRRRRYASQDDESK 72

Query: 61  GEMALRPS-PSPKRSLSS-ASSDGRRT-KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLI 120
            E  L PS   P R+LSS ASSDGRRT KRALLCGVTYKNWKHRL GTVNDV NMQDLLI
Sbjct: 73  PEKVLWPSVQPPMRTLSSTASSDGRRTIKRALLCGVTYKNWKHRLQGTVNDVRNMQDLLI 132

Query: 121 NHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDF 180
           NHFGY KQNIRILTEDE  PE++PTKKNIQ+ LKWLVEGCTGG++LVFYFSGHGLRQPDF
Sbjct: 133 NHFGYSKQNIRILTEDETKPEQMPTKKNIQNGLKWLVEGCTGGDNLVFYFSGHGLRQPDF 192

Query: 181 EMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYV 240
           +MDELDGYDETICPVDFMEEGMI+DNEINATIVSPLKNGV LHAIVDACHSGTILDLAYV
Sbjct: 193 DMDELDGYDETICPVDFMEEGMITDNEINATIVSPLKNGVTLHAIVDACHSGTILDLAYV 252

Query: 241 YDR-------NRPPSGARKATSGGLAISLSACEDDQFAADTS------------------ 289
           YDR       NRPPSGARK TSGGLAIS+SAC DDQFAADTS                  
Sbjct: 253 YDRDRDEWLDNRPPSGARKETSGGLAISMSACGDDQFAADTSILTGKTMNGAMTFILIHL 312

BLAST of Lsi03G007560 vs. NCBI nr
Match: XP_008455197.1 (PREDICTED: metacaspase-1 [Cucumis melo])

HSP 1 Score: 441.0 bits (1133), Expect = 7.7e-120
Identity = 251/367 (68.39%), Postives = 265/367 (72.21%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFK-CKC--NESFKP-SSSSSSGVG-SLRRRRNTSRDDE 60
           M +KSEYCCCKI KN+TKIN GFK CKC  N SFKP SSSSSSG G +LRRRR  S+DDE
Sbjct: 1   MYSKSEYCCCKINKNHTKINNGFKYCKCNNNHSFKPSSSSSSSGAGVALRRRRYASQDDE 60

Query: 61  SR--GEMALRPS--PSPKRSLSSASSDGRRT-KRALLCGVTYKNWKHRLHGTVNDVLNMQ 120
           SR   +  L PS  P P R+LSS SSDGRRT KRALLCGVTYKNWKHRL GTVNDV NMQ
Sbjct: 61  SRPDHQTVLWPSSVPPPTRTLSSTSSDGRRTIKRALLCGVTYKNWKHRLQGTVNDVRNMQ 120

Query: 121 DLLINHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLR 180
           DLLIN+FGY KQNIRILTEDE  PE++PTKKNIQ+ LKWLVEGCTGGE+LVFYFSGHGLR
Sbjct: 121 DLLINYFGYSKQNIRILTEDETKPEQIPTKKNIQNGLKWLVEGCTGGENLVFYFSGHGLR 180

Query: 181 QPDFEMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILD 240
           QPDF+MDELDGYDETICPVDFMEEGMI+DNEINATIVSPLKNGV LHAIVDACHSGTILD
Sbjct: 181 QPDFDMDELDGYDETICPVDFMEEGMITDNEINATIVSPLKNGVTLHAIVDACHSGTILD 240

Query: 241 LAYVYDR-------NRPPSGARKATSGGLAISLSACEDDQFAADTS-------------- 289
           LAYVYDR       NRPPSGARK TSGGLAIS+SAC DDQFAADTS              
Sbjct: 241 LAYVYDRNRNEWLDNRPPSGARKETSGGLAISMSACGDDQFAADTSILTGKTMNGAMTFI 300

BLAST of Lsi03G007560 vs. NCBI nr
Match: XP_023530043.1 (metacaspase-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.3 bits (1126), Expect = 5.0e-119
Identity = 243/357 (68.07%), Postives = 257/357 (71.99%), Query Frame = 0

Query: 1   MDTKSEYCCCKIIKNYTKINYGFKCKCNESFKPSSSSSSGVGSLRRRRNTSRDDESRGEM 60
           MDTKSE CCCKI  N+TK+   FKCKC + FKPSSSSSS VG+L     TSRDDES  EM
Sbjct: 1   MDTKSECCCCKIKNNHTKLT-PFKCKCIK-FKPSSSSSSDVGAL----YTSRDDESGAEM 60

Query: 61  ALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYY 120
            L+P PSP +SLSS++SDGR  KRALLCGV+YKNWKHRLHGTVNDVLNMQDLLINHF Y 
Sbjct: 61  DLQPPPSPMKSLSSSASDGRVRKRALLCGVSYKNWKHRLHGTVNDVLNMQDLLINHFSYL 120

Query: 121 KQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELD 180
           KQNIRILTEDEA PER+PTKKNIQSSLKWLVE C GGESLVFYFSGHGLRQPDF MDELD
Sbjct: 121 KQNIRILTEDEANPERIPTKKNIQSSLKWLVEDCYGGESLVFYFSGHGLRQPDFAMDELD 180

Query: 181 GYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAIVDACHSGTILDLAYVYDR--- 240
           GYDETICPVDF+EEGMISDNEINATIVSPL++GV LHAIVDACHSGTILDLAYVYDR   
Sbjct: 181 GYDETICPVDFLEEGMISDNEINATIVSPLRSGVNLHAIVDACHSGTILDLAYVYDRTRD 240

Query: 241 ----NRPPSGARKATSGGLAISLSACEDDQFAADTS------------------------ 289
               NRPPSGA KATSGGLAISLSAC DDQFAADTS                        
Sbjct: 241 EWIDNRPPSGASKATSGGLAISLSACGDDQFAADTSILTGKSMNGAMTFILIDLIKTCGN 300

BLAST of Lsi03G007560 vs. TAIR 10
Match: AT1G02170.1 (metacaspase 1 )

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-59
Identity = 108/197 (54.82%), Postives = 147/197 (74.62%), Query Frame = 0

Query: 83  KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKN 142
           KRA++CG++Y+  +H L G +ND   M+ LLIN F +   +I +LTE+E  P R+PTK+N
Sbjct: 80  KRAVICGISYRFSRHELKGCINDAKCMRHLLINKFKFSPDSILMLTEEETDPYRIPTKQN 139

Query: 143 IQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEI 202
           ++ +L WLV+GCT G+SLVF++SGHG RQ ++  DE+DGYDET+CP+DF  +GMI D+EI
Sbjct: 140 MRMALYWLVQGCTAGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLDFETQGMIVDDEI 199

Query: 203 NATIVSPLKNGVILHAIVDACHSGTILDL----------AYVYDRNRPPSGARKATSGGL 262
           NATIV PL +GV LH+I+DACHSGT+LDL           YV++ +RP SG  K T+GG 
Sbjct: 200 NATIVRPLPHGVKLHSIIDACHSGTVLDLPFLCRMNRAGQYVWEDHRPRSGLWKGTAGGE 259

Query: 263 AISLSACEDDQFAADTS 270
           AIS+S C+DDQ +ADTS
Sbjct: 260 AISISGCDDDQTSADTS 276

BLAST of Lsi03G007560 vs. TAIR 10
Match: AT4G25110.1 (metacaspase 2 )

HSP 1 Score: 199.1 bits (505), Expect = 4.7e-51
Identity = 105/204 (51.47%), Postives = 135/204 (66.18%), Query Frame = 0

Query: 83  KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKN 142
           KRA++ GV+YKN K  L G +ND   M+ +L+  F + +  I +LTE+EA P R PTK N
Sbjct: 116 KRAVIVGVSYKNTKDELKGCINDANCMKFMLMKRFQFPESCILMLTEEEADPMRWPTKNN 175

Query: 143 IQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEI 202
           I  ++ WLV  C  G+SLVF+FSGHG  Q D   DE+DG+DET+ PVD    G+I D+EI
Sbjct: 176 ITMAMHWLVLSCKPGDSLVFHFSGHGNNQMDDNGDEVDGFDETLLPVDHRTSGVIVDDEI 235

Query: 203 NATIVSPLKNGVILHAIVDACHSGTILDLAYV----------YDRNRPPSGARKATSGGL 262
           NATIV PL  GV LHAIVDACHSGT++DL Y+          ++ +RP +G  K TSGG 
Sbjct: 236 NATIVRPLPYGVKLHAIVDACHSGTVMDLPYLCRMDRLGNYEWEDHRPKTGMWKGTSGGE 295

Query: 263 AISLSACEDDQFAADTSEPQLSSS 277
             S + C+DDQ +ADT  PQLS S
Sbjct: 296 VFSFTGCDDDQTSADT--PQLSGS 317

BLAST of Lsi03G007560 vs. TAIR 10
Match: AT4G25110.2 (metacaspase 2 )

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-49
Identity = 105/204 (51.47%), Postives = 134/204 (65.69%), Query Frame = 0

Query: 83  KRALLCGVTYKNWKHRLHGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKN 142
           KRA++ GV+YKN K  L G +ND   M+ +L+  F + +  I +LTE EA P R PTK N
Sbjct: 116 KRAVIVGVSYKNTKDELKGCINDANCMKFMLMKRFQFPESCILMLTE-EADPMRWPTKNN 175

Query: 143 IQSSLKWLVEGCTGGESLVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEI 202
           I  ++ WLV  C  G+SLVF+FSGHG  Q D   DE+DG+DET+ PVD    G+I D+EI
Sbjct: 176 ITMAMHWLVLSCKPGDSLVFHFSGHGNNQMDDNGDEVDGFDETLLPVDHRTSGVIVDDEI 235

Query: 203 NATIVSPLKNGVILHAIVDACHSGTILDLAYV----------YDRNRPPSGARKATSGGL 262
           NATIV PL  GV LHAIVDACHSGT++DL Y+          ++ +RP +G  K TSGG 
Sbjct: 236 NATIVRPLPYGVKLHAIVDACHSGTVMDLPYLCRMDRLGNYEWEDHRPKTGMWKGTSGGE 295

Query: 263 AISLSACEDDQFAADTSEPQLSSS 277
             S + C+DDQ +ADT  PQLS S
Sbjct: 296 VFSFTGCDDDQTSADT--PQLSGS 316

BLAST of Lsi03G007560 vs. TAIR 10
Match: AT5G64240.1 (metacaspase 3 )

HSP 1 Score: 188.7 bits (478), Expect = 6.4e-48
Identity = 100/238 (42.02%), Postives = 142/238 (59.66%), Query Frame = 0

Query: 40  GVGSLRRRRNTSRDDESRGEMALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRL 99
           G   L R+      ++ + +M  +P P     L S        KRA+LCGV YK   + L
Sbjct: 51  GFQQLLRQHQPQHHEQQQQQMMAQPPPRLLEPLPSPFG----KKRAVLCGVNYKGKSYSL 110

Query: 100 HGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGES 159
            G ++D  +M+ LL+   G+   +I +LTEDEA P+R+PTK+NI+ +++WLVEG    +S
Sbjct: 111 KGCISDAKSMRSLLVQQMGFPIDSILMLTEDEASPQRIPTKRNIRKAMRWLVEGNRARDS 170

Query: 160 LVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAI 219
           LVF+FSGHG +Q D+  DE+DG DE +CP+D   EG I D+EIN  +V PL +G  LHA+
Sbjct: 171 LVFHFSGHGSQQNDYNGDEIDGQDEALCPLDHETEGKIIDDEINRILVRPLVHGAKLHAV 230

Query: 220 VDACHSGTILDLAYVYDRNRPPS---------GARKATSGGLAISLSACEDDQFAADT 269
           +DAC+SGT+LDL ++    R  S          A K T GG A   SAC+DD+ +  T
Sbjct: 231 IDACNSGTVLDLPFICRMERNGSYEWEDHRSVRAYKGTDGGAAFCFSACDDDESSGYT 284

BLAST of Lsi03G007560 vs. TAIR 10
Match: AT5G64240.2 (metacaspase 3 )

HSP 1 Score: 188.7 bits (478), Expect = 6.4e-48
Identity = 100/238 (42.02%), Postives = 142/238 (59.66%), Query Frame = 0

Query: 40  GVGSLRRRRNTSRDDESRGEMALRPSPSPKRSLSSASSDGRRTKRALLCGVTYKNWKHRL 99
           G   L R+      ++ + +M  +P P     L S        KRA+LCGV YK   + L
Sbjct: 51  GFQQLLRQHQPQHHEQQQQQMMAQPPPRLLEPLPSPFG----KKRAVLCGVNYKGKSYSL 110

Query: 100 HGTVNDVLNMQDLLINHFGYYKQNIRILTEDEAMPERLPTKKNIQSSLKWLVEGCTGGES 159
            G ++D  +M+ LL+   G+   +I +LTEDEA P+R+PTK+NI+ +++WLVEG    +S
Sbjct: 111 KGCISDAKSMRSLLVQQMGFPIDSILMLTEDEASPQRIPTKRNIRKAMRWLVEGNRARDS 170

Query: 160 LVFYFSGHGLRQPDFEMDELDGYDETICPVDFMEEGMISDNEINATIVSPLKNGVILHAI 219
           LVF+FSGHG +Q D+  DE+DG DE +CP+D   EG I D+EIN  +V PL +G  LHA+
Sbjct: 171 LVFHFSGHGSQQNDYNGDEIDGQDEALCPLDHETEGKIIDDEINRILVRPLVHGAKLHAV 230

Query: 220 VDACHSGTILDLAYVYDRNRPPS---------GARKATSGGLAISLSACEDDQFAADT 269
           +DAC+SGT+LDL ++    R  S          A K T GG A   SAC+DD+ +  T
Sbjct: 231 IDACNSGTVLDLPFICRMERNGSYEWEDHRSVRAYKGTDGGAAFCFSACDDDESSGYT 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XJE61.7e-5854.82Metacaspase-1 OS=Arabidopsis thaliana OX=3702 GN=AMC1 PE=1 SV=1[more]
Q7XJE56.7e-5051.47Metacaspase-2 OS=Arabidopsis thaliana OX=3702 GN=AMC2 PE=1 SV=1[more]
Q9FMG19.0e-4742.02Metacaspase-3 OS=Arabidopsis thaliana OX=3702 GN=AMC3 PE=2 SV=1[more]
Q2UN819.0e-3945.45Metacaspase-1A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=c... [more]
A2RB754.9e-3744.57Metacaspase-1A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 G... [more]
Match NameE-valueIdentityDescription
A0A0A0K2203.4e-12169.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G062910 PE=4 SV=1[more]
A0A1S3BZX63.7e-12068.39metacaspase-1 OS=Cucumis melo OX=3656 GN=LOC103495419 PE=4 SV=1[more]
A0A6J1EJD31.1e-11667.23metacaspase-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434681 PE=4... [more]
A0A6J1JNG91.9e-11667.04metacaspase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111486063 PE=4 SV=1[more]
A0A6J1EME32.8e-11580.80metacaspase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434681 PE=4... [more]
Match NameE-valueIdentityDescription
XP_038888839.19.6e-13975.98metacaspase-1-like [Benincasa hispida][more]
XP_004136878.16.9e-12169.42metacaspase-1 [Cucumis sativus][more]
KGN43776.26.9e-12169.42hypothetical protein Csa_017238 [Cucumis sativus][more]
XP_008455197.17.7e-12068.39PREDICTED: metacaspase-1 [Cucumis melo][more]
XP_023530043.15.0e-11968.07metacaspase-1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G02170.11.2e-5954.82metacaspase 1 [more]
AT4G25110.14.7e-5151.47metacaspase 2 [more]
AT4G25110.22.0e-4951.47metacaspase 2 [more]
AT5G64240.16.4e-4842.02metacaspase 3 [more]
AT5G64240.26.4e-4842.02metacaspase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.12660coord: 24..288
e-value: 1.8E-77
score: 263.0
NoneNo IPR availablePFAMPF00656Peptidase_C14coord: 83..271
e-value: 4.4E-39
score: 135.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..80
NoneNo IPR availablePANTHERPTHR48104:SF2ICE-LIKE PROTEASE (CASPASE) P20 DOMAIN PROTEINcoord: 41..269
NoneNo IPR availablePANTHERPTHR48104METACASPASE-4coord: 41..269
IPR029030Caspase-like domain superfamilySUPERFAMILY52129Caspase-likecoord: 78..192

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G007560.1Lsi03G007560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0004197 cysteine-type endopeptidase activity