Lsi03G005670 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G005670
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAmino acid permease family protein
Locationchr03: 6842130 .. 6844544 (-)
RNA-Seq ExpressionLsi03G005670
SyntenyLsi03G005670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTGGTTCCGTTTTCCATTTTTGTCTTTCAAGGACGCCACGGGAAGATGACAAAGTCAACCAAATCCCCATAATTCAACAAATTTCCCAAAGAGATTCGATTCGATACAGTGACCCAATTCCCCAAGATTCTTCTTCTGGGGTAATTCATTTCAATCCAGATTTAGCTTCACTTATTTGAAATTTCCATCCGATCTTCGTCATATCTCATTTCTGCTTCATCTTTCTGGTGGTTCTTCCCTACTAACAACAACCCTTCTTCTCCTTCCTGTCTATGGGGGACGACAGAAGGGTCGATGCTAAAATTGCTCCAAAACTAACCATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCATTTGGAGTGGAGGATTCGGTCAGCACTGGCGGAGGTCCGCTTCTTGCTTTGTTGGGTTTCTTAATTTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACTAGTTTCCCTCAAAATGGTGGGTATGTGGTCTGGATTTCGGCGGCTTTTGGCCCTTTTTGGGGTTTTCAAGAAGGGTTTTGGAAATGGTTTAGTGGGGTAATGGATAATGCTTTGTATCCTGTTTTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATGTATTTGCTCGAATTCCAGCTTTATTAGGAATCACTGCCTCTTTAACTTACTTAAACTATCGTGGTTTACACATTGTTGGGGTTTCTGCTGTTGTTCTTGCTGTCTTTTCACTTTGCCCTTTTGTGGTCATGACCCTTCTTTCAATTCCCAGAATAAGGCCTAAAAAGTGGCTAGTTGTAGAGTATAGTAAAGTGAATTGGAGGGGTTATTTCAACAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTTTAGCAGGGGAGGTTGAAAATCCTAGCAAAACTTTCCCTAAGGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCTTTGGAATCAGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGTTGGGGCCTTGATTGGTGGGGTTTGGCTGAAGTGGTGGATTCAAGCTGCTTCTGCTATGTCTAACATGGGATTGTTTGAGGCTGAAATGAGCAGTGATGCATATCAATTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGGTTAGTGGTTCTTTTCAGTTTTCTTTCATTGTAGACTGTATTGATAGCAAATTTGTAGCTCGAATTGGTAGTAGTGATTCAAAATAGCAATCCTTCGGGTCGAAGGTTTAAGCTTCGAATCGGTAGTAGTGATTCAAAACTATAACAGTCCTAGGGGTGTATTTAGCTTCCAATATGAGTTTGTATAGCTCAAGATTATAACATACATGTTTGTTAATCCCACATACTACTTAAACTCAGGTTATGTAAATTCAAAGTCAGGTACCATTCTACTGCCTATGTCTATCTCCAAGTATGGGTCTATACAAACTCAAACTTTTCAACCTTGGACTTGTTAAAACCTTAAGCCAAACATTTCCTAAACTCTTCAGCATTTTGAGCCATTTGATTTACGGCACATTGTGGATACAAAATGTTAAAATGCCTTGAATCTGTTATCTGGCGTTTCAAAACCTATTTTGTATTCGTATTTATGTTATTTACCTTCGAATCCTATAGATGCCTTTACAATATGTATTATCTTATTTTCTTTCTAATAAAATCTAATCTCTCTCTGCTTAGCCAACACACTGTTAGTGAACCACGTAAATCTCTGTGTTGAATTCTCTACTGTTTACTTTACGTGCTTTCTTTGATTTGTCGATTTCATAACATAAAAGCGGTCTCTTATTCACTTTGCAGATCCAAATATGGGACACCCACCTTCAGCGTTTTGTGCTCTGCCATGGGGGTTATCTTTCTCTCATGGATGAGCTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCTGTAGGAATGCTTTTGGAGTTTGCTGCCTTTATAAAACTAAGAATAAAGAAGCCAGACCTGAACAGACCCTACAGAGTTCCCTTGCAAACATTTGGTGTCACAATGCTTTGCTTCCCACCTGCAGCCTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCAAAAACATTCTTAATCAGTGGGATTATTGTAGTTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAAGCAAAGAACAGAAGATGGGTCAAATTTATTTCAGAACAACCAGAAGCTACTCTTCCTGATGTGGAAGATAGGCTGGTCGAGCCGCAACCGCAGCTAGAAGTTCCCAATGAGGCAGAGGTTCGGCTTCTTTCAGAATCTTCTTCGTCTTCGAATATAGCCCAGCAATAA

mRNA sequence

GGTGGTTCCGTTTTCCATTTTTGTCTTTCAAGGACGCCACGGGAAGATGACAAAGTCAACCAAATCCCCATAATTCAACAAATTTCCCAAAGAGATTCGATTCGATACAGTGACCCAATTCCCCAAGATTCTTCTTCTGGGGTAATTCATTTCAATCCAGATTTAGCTTCACTTATTTGAAATTTCCATCCGATCTTCGTCATATCTCATTTCTGCTTCATCTTTCTGGTGGTTCTTCCCTACTAACAACAACCCTTCTTCTCCTTCCTGTCTATGGGGGACGACAGAAGGGTCGATGCTAAAATTGCTCCAAAACTAACCATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCATTTGGAGTGGAGGATTCGGTCAGCACTGGCGGAGGTCCGCTTCTTGCTTTGTTGGGTTTCTTAATTTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACTAGTTTCCCTCAAAATGGTGGGTATGTGGTCTGGATTTCGGCGGCTTTTGGCCCTTTTTGGGGTTTTCAAGAAGGGTTTTGGAAATGGTTTAGTGGGGTAATGGATAATGCTTTGTATCCTGTTTTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATGTATTTGCTCGAATTCCAGCTTTATTAGGAATCACTGCCTCTTTAACTTACTTAAACTATCGTGGTTTACACATTGTTGGGGTTTCTGCTGTTGTTCTTGCTGTCTTTTCACTTTGCCCTTTTGTGGTCATGACCCTTCTTTCAATTCCCAGAATAAGGCCTAAAAAGTGGCTAGTTGTAGAGTATAGTAAAGTGAATTGGAGGGGTTATTTCAACAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTTTAGCAGGGGAGGTTGAAAATCCTAGCAAAACTTTCCCTAAGGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCTTTGGAATCAGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGTTGGGGCCTTGATTGGTGGGGTTTGGCTGAAGTGGTGGATTCAAGCTGCTTCTGCTATGTCTAACATGGGATTGTTTGAGGCTGAAATGAGCAGTGATGCATATCAATTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGCGTTTTGTGCTCTGCCATGGGGGTTATCTTTCTCTCATGGATGAGCTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCTGTAGGAATGCTTTTGGAGTTTGCTGCCTTTATAAAACTAAGAATAAAGAAGCCAGACCTGAACAGACCCTACAGAGTTCCCTTGCAAACATTTGGTGTCACAATGCTTTGCTTCCCACCTGCAGCCTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCAAAAACATTCTTAATCAGTGGGATTATTGTAGTTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAAGCAAAGAACAGAAGATGGGTCAAATTTATTTCAGAACAACCAGAAGCTACTCTTCCTGATGTGGAAGATAGGCTGGTCGAGCCGCAACCGCAGCTAGAAGTTCCCAATGAGGCAGAGGTTCGGCTTCTTTCAGAATCTTCTTCGTCTTCGAATATAGCCCAGCAATAA

Coding sequence (CDS)

ATGGGGGACGACAGAAGGGTCGATGCTAAAATTGCTCCAAAACTAACCATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCATTTGGAGTGGAGGATTCGGTCAGCACTGGCGGAGGTCCGCTTCTTGCTTTGTTGGGTTTCTTAATTTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACTAGTTTCCCTCAAAATGGTGGGTATGTGGTCTGGATTTCGGCGGCTTTTGGCCCTTTTTGGGGTTTTCAAGAAGGGTTTTGGAAATGGTTTAGTGGGGTAATGGATAATGCTTTGTATCCTGTTTTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATGTATTTGCTCGAATTCCAGCTTTATTAGGAATCACTGCCTCTTTAACTTACTTAAACTATCGTGGTTTACACATTGTTGGGGTTTCTGCTGTTGTTCTTGCTGTCTTTTCACTTTGCCCTTTTGTGGTCATGACCCTTCTTTCAATTCCCAGAATAAGGCCTAAAAAGTGGCTAGTTGTAGAGTATAGTAAAGTGAATTGGAGGGGTTATTTCAACAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTTTAGCAGGGGAGGTTGAAAATCCTAGCAAAACTTTCCCTAAGGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCTTTGGAATCAGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGTTGGGGCCTTGATTGGTGGGGTTTGGCTGAAGTGGTGGATTCAAGCTGCTTCTGCTATGTCTAACATGGGATTGTTTGAGGCTGAAATGAGCAGTGATGCATATCAATTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGCGTTTTGTGCTCTGCCATGGGGGTTATCTTTCTCTCATGGATGAGCTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCTGTAGGAATGCTTTTGGAGTTTGCTGCCTTTATAAAACTAAGAATAAAGAAGCCAGACCTGAACAGACCCTACAGAGTTCCCTTGCAAACATTTGGTGTCACAATGCTTTGCTTCCCACCTGCAGCCTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCAAAAACATTCTTAATCAGTGGGATTATTGTAGTTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAAGCAAAGAACAGAAGATGGGTCAAATTTATTTCAGAACAACCAGAAGCTACTCTTCCTGATGTGGAAGATAGGCTGGTCGAGCCGCAACCGCAGCTAGAAGTTCCCAATGAGGCAGAGGTTCGGCTTCTTTCAGAATCTTCTTCGTCTTCGAATATAGCCCAGCAATAA

Protein sequence

MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGMIPSVFASSVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQAKNRRWVKFISEQPEATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSSSSNIAQQ
Homology
BLAST of Lsi03G005670 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 4.4e-200
Identity = 347/451 (76.94%), Postives = 393/451 (87.14%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           + D+     K +PKLT+LPL+ LIFY+VSGGPFGVEDSV +GGGPLLALLGFLIFP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
            SFPV +HV AR+PALL IT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM LL++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPK+WL V+  K+NWRGYFN+MFWNLNYWDKASTLAGEV+ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESDSS-EWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S +S EWSDGYFAEVG LIGGVWLK WIQAA+AMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGM 360
           A+QLLGMSE+GM+P+ FA          S+LCSA GVIFLSWMSFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIV 420
           LLEFAAF+KLRIKKPDL+RPYRVPL TFGV+MLC PP+ L++LVM LA+ KTFLISG+I+
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 VVGFLLYPTLLQAKNRRWVKFISEQPEATLP 442
           V+GF LYP L   K ++W +FI   PE T P
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFI---PEETRP 455

BLAST of Lsi03G005670 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.1e-145
Identity = 246/429 (57.34%), Postives = 324/429 (75.52%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATS 73
           K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF++FPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW SGV+DNALYP+LFLDYLK   P+      R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVV--EYS 193
            A+L +T +LTYLNYRGL IVGV+AV+L VFS+ PFVVM+ +SIP+++P +WLVV  +  
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  STL GEVENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGM 313
           +  D   W+DGYFA++G +IGGVWL WWIQAA+A SNMG+F AEMSSD++QLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRI 373
           +P VFA           +L SA GVI LSW+SFQEI+   N LY  GM+LEF  F++LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQ 432
           K P  +RP+++P+   G  ++C PP  L+ ++M   + K  L+S   +V+G +L P L Q
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

BLAST of Lsi03G005670 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.0e-144
Identity = 250/450 (55.56%), Postives = 332/450 (73.78%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF+IFPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYSKV 193
            ++L +T  LTYLNYRGL IVG  AV++ VFS+ PF VM L+SIP++ P +WLV++   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGEVENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++V   +GG WL+WW+QAA+A SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIKK 373
             FA           +L SA GV+ LSW+SFQEI+   N LY VGM+LEF AF+++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G  ++C PP  L+  V+ L+S K   +S +++++GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 NRRWVKFISEQPEATLPDVEDRLVEPQPQL 455
            +RWVKF      + LPD++ +  E +  L
Sbjct: 468 RKRWVKF---SISSDLPDLQQQTREYEETL 493

BLAST of Lsi03G005670 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.5e-139
Identity = 249/441 (56.46%), Postives = 325/441 (73.70%), Query Frame = 0

Query: 12  APKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELA 71
           A  ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GFL+ P IWSIPEAL+TAEL 
Sbjct: 80  ARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSIPEALITAELG 139

Query: 72  TSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFA 131
             FP+NGGYVVW+++A GP+WGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       
Sbjct: 140 AMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAP 199

Query: 132 RIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYS 191
           R  A++G+TA LT LNYRGL +VG  A+ L VFSL PF VM L+++P++RP +WLV++  
Sbjct: 200 RAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLH 259

Query: 192 KVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 251
            V+W  Y N++FWNLNYWD  STLAGEV+NP KT PKA+F AV+ VV +YL PLLAGTGA
Sbjct: 260 NVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGA 319

Query: 252 LESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGM 311
           +  D  +W+DGYFA++  L+GG WL WW+Q+A+A+SNMG+F AEMSSD+YQLLGM+E GM
Sbjct: 320 VPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGM 379

Query: 312 IPSVFA---------SSVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRI 371
           +PS FA         + +L SA GV+ LS MSFQEI+   NFLY  GMLLEF AFI  R+
Sbjct: 380 LPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRV 439

Query: 372 KKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQ 431
           ++PD  RPYRVPL T G   +  PP AL+ +V+ L++ K  ++S   V +G +L P L  
Sbjct: 440 RRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRF 499

Query: 432 AKNRRWVKFISEQPEATLPDV 444
            + +RW++F S  P+  LP++
Sbjct: 500 VEKKRWLRF-SVNPD--LPEI 516

BLAST of Lsi03G005670 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.5e-139
Identity = 249/441 (56.46%), Postives = 325/441 (73.70%), Query Frame = 0

Query: 12  APKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELA 71
           A  ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GFL+ P IWSIPEAL+TAEL 
Sbjct: 80  ARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSIPEALITAELG 139

Query: 72  TSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFA 131
             FP+NGGYVVW+++A GP+WGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       
Sbjct: 140 AMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAP 199

Query: 132 RIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYS 191
           R  A++G+TA LT LNYRGL +VG  A+ L VFSL PF VM L+++P++RP +WLV++  
Sbjct: 200 RAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLH 259

Query: 192 KVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 251
            V+W  Y N++FWNLNYWD  STLAGEV+NP KT PKA+F AV+ VV +YL PLLAGTGA
Sbjct: 260 NVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGA 319

Query: 252 LESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGM 311
           +  D  +W+DGYFA++  L+GG WL WW+Q+A+A+SNMG+F AEMSSD+YQLLGM+E GM
Sbjct: 320 VPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGM 379

Query: 312 IPSVFA---------SSVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRI 371
           +PS FA         + +L SA GV+ LS MSFQEI+   NFLY  GMLLEF AFI  R+
Sbjct: 380 LPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRV 439

Query: 372 KKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQ 431
           ++PD  RPYRVPL T G   +  PP AL+ +V+ L++ K  ++S   V +G +L P L  
Sbjct: 440 RRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRF 499

Query: 432 AKNRRWVKFISEQPEATLPDV 444
            + +RW++F S  P+  LP++
Sbjct: 500 VEKKRWLRF-SVNPD--LPEI 516

BLAST of Lsi03G005670 vs. ExPASy TrEMBL
Match: A0A5D3C4H2 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001140 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.2e-252
Identity = 450/487 (92.40%), Postives = 466/487 (95.69%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RRVDAKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
            PKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRI+KPDLNRPY+VPLQT GVT+LCFPP+ALL+LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPE-ATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSS 478
           VGFLLYPTLLQAKNRRWVKFISEQPE  TL DVEDRLVEPQPQ EVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSE-SS 480

BLAST of Lsi03G005670 vs. ExPASy TrEMBL
Match: A0A1S3C014 (probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495296 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.2e-251
Identity = 449/487 (92.20%), Postives = 466/487 (95.69%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RRVDAKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
            PKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRI+KPDLNRPY+VPLQT GVT+LCFPP+ALL+LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPE-ATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSS 478
           VGFLLYPTLLQAKNRRWVKFISEQPE  TL DVEDRLVEPQPQ EVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSE-SS 480

BLAST of Lsi03G005670 vs. ExPASy TrEMBL
Match: A0A0A0K2Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.4e-251
Identity = 449/487 (92.20%), Postives = 464/487 (95.28%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RRVDAKI  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
            PKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE+DSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGM+PSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLY++GML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRI+KPDLNRPY+VPLQTFGVT+LCFPPAALL LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPE-ATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSS 478
           VGFLLYPTLLQAKNRRWVKFISEQPE  TLPDVEDRLVE Q Q EVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSE-SS 480

BLAST of Lsi03G005670 vs. ExPASy TrEMBL
Match: A0A6J1I9Z5 (probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC111471008 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.2e-241
Identity = 431/486 (88.68%), Postives = 453/486 (93.21%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+R   AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDER---AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVF+H+FARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLVV+YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSS+WSDGYFAEVGALIGG WLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LE AAFIKLRIKKPDL+RPY+VPLQTFG  MLC PPAALLVLVMCLASA+TFLISGII+ 
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIIS 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSSS 478
           VGFLLYP+L QAKNR WVKFISEQ   TLPDVED LVEPQP+ EV +EA +RLLSES SS
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDEAVIRLLSESPSS 480

BLAST of Lsi03G005670 vs. ExPASy TrEMBL
Match: A0A6J1GKM4 (probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111455227 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.0e-239
Identity = 428/486 (88.07%), Postives = 451/486 (92.80%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+R   A I PKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVF+H+FARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLVV+YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSSEWSDGYFAEVGALIGG WLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LE AAFIKLRIKKPDL+RPY+VPLQTFG  MLC PPAALLVLVMCLASA+TFLISG+I+ 
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSSS 478
           VGFLLYP+L QAKNR WVKFISEQ   TLPDVED LVEPQP+ EV +E+ +RLLSES SS
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSS 480

BLAST of Lsi03G005670 vs. NCBI nr
Match: XP_038888395.1 (probable polyamine transporter At3g19553 [Benincasa hispida])

HSP 1 Score: 900.6 bits (2326), Expect = 5.9e-258
Identity = 461/486 (94.86%), Postives = 468/486 (96.30%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RR DAKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRADAKIAQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHVFARIP LLGITASLTYLNYRGLHIVGVSAVVLA FSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHVFARIPTLLGITASLTYLNYRGLHIVGVSAVVLAAFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGM+PSVFA+         S+LCSAMGVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMLPSVFAARSKYGTPTFSILCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIV 
Sbjct: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVT 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSSS 478
           VGFLLYPTLLQAKN+RWVKFISEQPEATLPDVEDRLVE   Q EVPNEAEVRLLSESSSS
Sbjct: 421 VGFLLYPTLLQAKNKRWVKFISEQPEATLPDVEDRLVESHAQQEVPNEAEVRLLSESSSS 480

BLAST of Lsi03G005670 vs. NCBI nr
Match: KAA0031359.1 (putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 879.8 bits (2272), Expect = 1.1e-251
Identity = 450/487 (92.40%), Postives = 466/487 (95.69%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RRVDAKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
            PKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRI+KPDLNRPY+VPLQT GVT+LCFPP+ALL+LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPE-ATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSS 478
           VGFLLYPTLLQAKNRRWVKFISEQPE  TL DVEDRLVEPQPQ EVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSE-SS 480

BLAST of Lsi03G005670 vs. NCBI nr
Match: XP_008455023.1 (PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo])

HSP 1 Score: 878.6 bits (2269), Expect = 2.4e-251
Identity = 449/487 (92.20%), Postives = 466/487 (95.69%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RRVDAKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
            PKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRI+KPDLNRPY+VPLQT GVT+LCFPP+ALL+LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPE-ATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSS 478
           VGFLLYPTLLQAKNRRWVKFISEQPE  TL DVEDRLVEPQPQ EVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSE-SS 480

BLAST of Lsi03G005670 vs. NCBI nr
Match: XP_004136943.1 (probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypothetical protein Csa_017296 [Cucumis sativus])

HSP 1 Score: 877.1 bits (2265), Expect = 6.9e-251
Identity = 449/487 (92.20%), Postives = 464/487 (95.28%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+RRVDAKI  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
            PKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE+DSSEWSDGYFAEVGALIGGVWLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGM+PSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLY++GML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LEFAAFIKLRI+KPDLNRPY+VPLQTFGVT+LCFPPAALL LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPE-ATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSS 478
           VGFLLYPTLLQAKNRRWVKFISEQPE  TLPDVEDRLVE Q Q EVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSE-SS 480

BLAST of Lsi03G005670 vs. NCBI nr
Match: XP_023553804.1 (probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 843.6 bits (2178), Expect = 8.5e-241
Identity = 429/486 (88.27%), Postives = 454/486 (93.42%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           MGD+R   A IAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDER---ANIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVF+H+FARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLVV+YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSSEWSDGYFAEVGALIGG WLKWWIQAA+AMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFAS         S+LCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVV 420
           LE AAFIKLR+KKPDL+RPY+VPLQTFG  MLC PPAALLVLVMCLASA+TFLISG+I+ 
Sbjct: 361 LELAAFIKLRMKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEATLPDVEDRLVEPQPQLEVPNEAEVRLLSESSSS 478
           VGFLLYP+L QAKNR WVKFIS++  ATLPDVEDRLVEPQP+ EV +EA +RLLSES SS
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISDEQGATLPDVEDRLVEPQPREEVLDEAVIRLLSESPSS 480

BLAST of Lsi03G005670 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 698.7 bits (1802), Expect = 3.2e-201
Identity = 347/451 (76.94%), Postives = 393/451 (87.14%), Query Frame = 0

Query: 1   MGDDRRVDAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWS 60
           + D+     K +PKLT+LPL+ LIFY+VSGGPFGVEDSV +GGGPLLALLGFLIFP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHVFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
            SFPV +HV AR+PALL IT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM LL++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPK+WL V+  K+NWRGYFN+MFWNLNYWDKASTLAGEV+ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESDSS-EWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S +S EWSDGYFAEVG LIGGVWLK WIQAA+AMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGM 360
           A+QLLGMSE+GM+P+ FA          S+LCSA GVIFLSWMSFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIV 420
           LLEFAAF+KLRIKKPDL+RPYRVPL TFGV+MLC PP+ L++LVM LA+ KTFLISG+I+
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 VVGFLLYPTLLQAKNRRWVKFISEQPEATLP 442
           V+GF LYP L   K ++W +FI   PE T P
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFI---PEETRP 455

BLAST of Lsi03G005670 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 6.5e-146
Identity = 246/429 (57.34%), Postives = 324/429 (75.52%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATS 73
           K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF++FPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW SGV+DNALYP+LFLDYLK   P+      R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVV--EYS 193
            A+L +T +LTYLNYRGL IVGV+AV+L VFS+ PFVVM+ +SIP+++P +WLVV  +  
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  STL GEVENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGM 313
           +  D   W+DGYFA++G +IGGVWL WWIQAA+A SNMG+F AEMSSD++QLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRI 373
           +P VFA           +L SA GVI LSW+SFQEI+   N LY  GM+LEF  F++LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQ 432
           K P  +RP+++P+   G  ++C PP  L+ ++M   + K  L+S   +V+G +L P L Q
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

BLAST of Lsi03G005670 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-145
Identity = 250/450 (55.56%), Postives = 332/450 (73.78%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF+IFPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYSKV 193
            ++L +T  LTYLNYRGL IVG  AV++ VFS+ PF VM L+SIP++ P +WLV++   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGEVENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++V   +GG WL+WW+QAA+A SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIKK 373
             FA           +L SA GV+ LSW+SFQEI+   N LY VGM+LEF AF+++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G  ++C PP  L+  V+ L+S K   +S +++++GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 NRRWVKFISEQPEATLPDVEDRLVEPQPQL 455
            +RWVKF      + LPD++ +  E +  L
Sbjct: 468 RKRWVKF---SISSDLPDLQQQTREYEETL 493

BLAST of Lsi03G005670 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-145
Identity = 250/450 (55.56%), Postives = 332/450 (73.78%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF+IFPFIWSIPEAL+TAE+ T 
Sbjct: 32  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 91

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R+
Sbjct: 92  YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 151

Query: 134 PALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYSKV 193
            ++L +T  LTYLNYRGL IVG  AV++ VFS+ PF VM L+SIP++ P +WLV++   V
Sbjct: 152 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 211

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGEVENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 212 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 271

Query: 254 SDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++V   +GG WL+WW+QAA+A SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 272 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 331

Query: 314 SVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIKK 373
             FA           +L SA GV+ LSW+SFQEI+   N LY VGM+LEF AF+++R+K 
Sbjct: 332 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 391

Query: 374 PDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G  ++C PP  L+  V+ L+S K   +S +++++GFL++P L    
Sbjct: 392 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 451

Query: 434 NRRWVKFISEQPEATLPDVEDRLVEPQPQL 455
            +RWVKF      + LPD++ +  E +  L
Sbjct: 452 RKRWVKF---SISSDLPDLQQQTREYEETL 477

BLAST of Lsi03G005670 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.1e-134
Identity = 238/427 (55.74%), Postives = 311/427 (72.83%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLIFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFG E SV+   GPLLALLGF+IFPFIW IPEAL+TAE++T 
Sbjct: 35  KVSMLPLVFLIFYEVSGGPFGAEGSVN-AAGPLLALLGFVIFPFIWCIPEALITAEMSTM 94

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHVFARI 133
           FP NGG+VVW+S+A G FWGFQ G+ KW  GV+DNALYPVLFLDYLK + P       R+
Sbjct: 95  FPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPALATGLPRV 154

Query: 134 PALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRIRPKKWLVVEYSKV 193
            ++L +T  LTYLNYRGL IVG +AV + VFS+ PF VM+L+SIP++ P +WLV++   V
Sbjct: 155 ASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWLVMDLGNV 214

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++ WNLNYWD  STLAGEV NP KT PKA+   V+ V  S  +PLL+GTGA+ 
Sbjct: 215 NWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLLSGTGAIP 274

Query: 254 SDSSEWSDGYFAEVGALIGGVWLKWWIQAASAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            D   W+DGY AEV   IGG WL+ W+QAA+A SNMG+F AEMSSD++QLLGM+E+G++P
Sbjct: 275 LDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGMAELGILP 334

Query: 314 SVFAS---------SVLCSAMGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIKK 373
            +FA           +L SA GV+ LS +SFQEI+   N LY  GM+LEF AF++LR K 
Sbjct: 335 EIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAFVRLRKKH 394

Query: 374 PDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVVVGFLLYPTLLQAK 432
           P  +RPY++P+ T G  ++C PP  L+ LV+ L++ K  L+S ++VV+GFL+ P L    
Sbjct: 395 PAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGFLMKPCLNHMD 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LH394.4e-20076.94Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9FFL19.1e-14557.34Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9C6S52.0e-14455.56Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A2X8M81.5e-13956.46Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D01.5e-13956.46Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3C4H25.2e-25292.40Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C0141.2e-25192.20probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0K2Y23.4e-25192.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1[more]
A0A6J1I9Z59.2e-24188.68probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1GKM41.0e-23988.07probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038888395.15.9e-25894.86probable polyamine transporter At3g19553 [Benincasa hispida][more]
KAA0031359.11.1e-25192.40putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative p... [more]
XP_008455023.12.4e-25192.20PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo][more]
XP_004136943.16.9e-25192.20probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypotheti... [more]
XP_023553804.18.5e-24188.27probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G19553.13.2e-20176.94Amino acid permease family protein [more]
AT5G05630.16.5e-14657.34Amino acid permease family protein [more]
AT1G31830.11.4e-14555.56Amino acid permease family protein [more]
AT1G31830.21.4e-14555.56Amino acid permease family protein [more]
AT1G31820.11.1e-13455.74Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 11..454
e-value: 5.4E-58
score: 194.1
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 14..435
e-value: 6.0E-52
score: 178.7
NoneNo IPR availablePANTHERPTHR45826:SF21BNAA05G20530D PROTEINcoord: 4..440
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 20..413
e-value: 2.3E-24
score: 86.0
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 4..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G005670.1Lsi03G005670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity