Lsi03G003970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G003970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	50S ribosomal protein L27
Location	chr03: 4561547 .. 4562923 (-)
RNA-Seq Expression	Lsi03G003970
Synteny	Lsi03G003970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGCGTTTCATTTTAGCCTCGAGTCTGGCTTTACGAGTTTGTGTTGTGCAGTGCAGGCTTTGTGGCGATCCTGCTTCTAACTTCTAAGGATAAGGTTCGCATTCACTCCCCTCTGTCTCTTTCCCTCCTCCATATCCATTTCCTTTCTCTATTAACTCATTCTCTGAATCTGAAACCTCAATCAATTTCTTGTCTTCCCCCAAGAATTCCCCGTTGCAGCCATGAGTTTAAACCTAGTTGCTGCATTCACTGGTCTCTCTCTCGCCTCTTCTTCCTCTTTCTTCAAAGGCGATTTCGGCTCTATTCAGCCCTCTCCCAAGCTCTCTGTCTCCTTCCCCAACAGATTCCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGAAGCACCAAGAACGGCCGTGATTCCAAGGGCCAGAGACTCGGCGTTAAAATCTTCGGCGACCAAGTTGCTAAGCCTGGTTCCATCATCGTTCGTCAGCGTGGAACTAAGGTAATTTTGAATTTTACATGGAGTTGATTCCTATGGTACCCGTTTCTAGGGCATTCTCCAGCTACATACATCCAACTTCTTTGTAATTGAGTCCTATGGTTTCTGTTTCTGTACCAATCATTTCAGTTTCATGCCGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGGCTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTTTCACTTCGAATCTGAAAGGTTATATTTCAAGAATGAAACACAAAACTGAGATATATTTATTTACTTCATCGTTCATTGCATTGCAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGTTCAACCCCAATTAGTATTAGCTTCCACTGATGCTACTGAAACCAATCCAGTTTGCTGATTTATAAAGCCAGAATTTCAATCACTGCTTCTTTAAAATCCCTGATGATCTTGCAGATAGAAGGTCTGCTTCATTAGCCTCTCTTTTTTGGTTGTGCACATTTATTAATTTCCCAGATTCTTCACAGGAATGTGTTTCATTATTATGATTCTCAATTAGCAATACTCGTTTTGTTCTACGTTTTGCCTATACATTTTCTCTTTAGTCCATTCCACTACTTACTACTTTTTGTTGATTTTGATGTCAAAAATGGTATTCAACAGAACAGATAATAGGGGTGAGTTTTGATTTATTTTGTGTTGAAATTACAAACCAAGAAATTAGATGGATTTGGTGGTTCTTCCCTGTACCAACATTACTAGACTGTGATATGAATCATGTAGCTTTGG

mRNA sequence

GGGCGTTTCATTTTAGCCTCGAGTCTGGCTTTACGAGTTTGTGTTGTGCAGTGCAGGCTTTGTGGCGATCCTGCTTCTAACTTCTAAGGATAAGAATTCCCCGTTGCAGCCATGAGTTTAAACCTAGTTGCTGCATTCACTGGTCTCTCTCTCGCCTCTTCTTCCTCTTTCTTCAAAGGCGATTTCGGCTCTATTCAGCCCTCTCCCAAGCTCTCTGTCTCCTTCCCCAACAGATTCCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGAAGCACCAAGAACGGCCGTGATTCCAAGGGCCAGAGACTCGGCGTTAAAATCTTCGGCGACCAAGTTGCTAAGCCTGGTTCCATCATCGTTCGTCAGCGTGGAACTAAGTTTCATGCCGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGGCTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGTTCAACCCCAATTAGTATTAGCTTCCACTGATGCTACTGAAACCAATCCAGTTTGCTGATTTATAAAGCCAGAATTTCAATCACTGCTTCTTTAAAATCCCTGATGATCTTGCAGATAGAAGGTCTGCTTCATTAGCCTCTCTTTTTTGGTTGTGCACATTTATTAATTTCCCAGATTCTTCACAGGAATGTGTTTCATTATTATGATTCTCAATTAGCAATACTCGTTTTGTTCTACGTTTTGCCTATACATTTTCTCTTTAGTCCATTCCACTACTTACTACTTTTTGTTGATTTTGATGTCAAAAATGGTATTCAACAGAACAGATAATAGGGGTGAGTTTTGATTTATTTTGTGTTGAAATTACAAACCAAGAAATTAGATGGATTTGGTGGTTCTTCCCTGTACCAACATTACTAGACTGTGATATGAATCATGTAGCTTTGG

Coding sequence (CDS)

ATGAGTTTAAACCTAGTTGCTGCATTCACTGGTCTCTCTCTCGCCTCTTCTTCCTCTTTCTTCAAAGGCGATTTCGGCTCTATTCAGCCCTCTCCCAAGCTCTCTGTCTCCTTCCCCAACAGATTCCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGAAGCACCAAGAACGGCCGTGATTCCAAGGGCCAGAGACTCGGCGTTAAAATCTTCGGCGACCAAGTTGCTAAGCCTGGTTCCATCATCGTTCGTCAGCGTGGAACTAAGTTTCATGCCGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGGCTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGTTCAACCCCAATTAGTATTAGCTTCCACTGATGCTACTGAAACCAATCCAGTTTGCTGA

Protein sequence

MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETNPVC

Homology

[Close]

BLAST of Lsi03G003970 vs. ExPASy Swiss-Prot
Match: P30155 (50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL27 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 1.8e-72
Identity = 140/167 (83.83%), Postives = 153/167 (91.62%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQP-SPKLSVSFPNRFPLTIENAHKKGAGSTKN 60
           +S +LV AF GLSLASSSSF KGDFG+  P +PK SVSFP + PLTIE+AHKKGAGSTKN
Sbjct: 3   VSFSLVGAFKGLSLASSSSFLKGDFGAAFPVAPKFSVSFPLKSPLTIESAHKKGAGSTKN 62

Query: 61  GRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKY 120
           GRDS GQRLGVKIFGDQVAKPGSIIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+
Sbjct: 63  GRDSPGQRLGVKIFGDQVAKPGSIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKF 122

Query: 121 GPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQ 167
           GPD+KK+SVYPREVQPENPNSYR RKRE FRLQRER+KAR+EG+I+Q
Sbjct: 123 GPDRKKISVYPREVQPENPNSYRNRKRESFRLQRERRKARREGVILQ 169

[Close]

BLAST of Lsi03G003970 vs. ExPASy Swiss-Prot
Match: P82190 (50S ribosomal protein L27, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL27 PE=1 SV=2)

HSP 1 Score: 269.6 bits (688), Expect = 2.5e-71
Identity = 143/188 (76.06%), Postives = 161/188 (85.64%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSL--ASSSSFFKGDFG-SIQPSPKLSVSFPNRFPLTIENAHKKGAGST 60
           MS NL+A+F G+SL  +SSSSFFKG+FG S    P  S    + FPLTIE+AHKKGAGST
Sbjct: 7   MSFNLMASFRGMSLSSSSSSSFFKGEFGPSSLRLPNKSPLSVSPFPLTIESAHKKGAGST 66

Query: 61  KNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFE 120
           KNGRDSKGQRLGVKI+GDQVAKPG+II+RQRGTKFH GKNVG+GKDHTIF+LIDGLVKFE
Sbjct: 67  KNGRDSKGQRLGVKIYGDQVAKPGAIIIRQRGTKFHPGKNVGIGKDHTIFALIDGLVKFE 126

Query: 121 KYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLAS--TD 180
           KYGPDKKKVSVYPRE+QPENPNSYRARKRE FRLQRE+KKAR+EG   QPQL+LAS  TD
Sbjct: 127 KYGPDKKKVSVYPREIQPENPNSYRARKRENFRLQREKKKARREGYSFQPQLILASAATD 186

Query: 181 ATETNPVC 184
             + + VC
Sbjct: 187 NADESAVC 194

[Close]

BLAST of Lsi03G003970 vs. ExPASy Swiss-Prot
Match: Q9FLN4 (50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 5.9e-68
Identity = 138/193 (71.50%), Postives = 156/193 (80.83%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNR-----FPLTIENAHKKGAG 60
           MSLNL+ AF GLSL+S+SSF +GD  S  P    +V+ P        PLTIE+AHKKGAG
Sbjct: 7   MSLNLIGAFKGLSLSSTSSFLRGDL-SFSPKTSFTVTLPLENLQAPIPLTIESAHKKGAG 66

Query: 61  STKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVK 120
           STKNGRDS GQRLGVKI+GDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLIDGLVK
Sbjct: 67  STKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLIDGLVK 126

Query: 121 FEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---IIVQPQLVL- 180
           FEK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E     +  P+LVL 
Sbjct: 127 FEKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTPELVLA 186

Query: 181 -ASTDATETNPVC 184
            AS D  E NP C
Sbjct: 187 SASVDDAEANPEC 198

[Close]

BLAST of Lsi03G003970 vs. ExPASy Swiss-Prot
Match: O65037 (50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.0e-60
Identity = 129/193 (66.84%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSL------ASSSSFFKGDFGSIQPSPKLSVSFPNRFP---LTIENAHK 60
           M+  LV AF G+SL      +SS+SF + D  S+     + +  P   P   LTI+ AHK
Sbjct: 4   MAFTLVGAFKGMSLSSPCHSSSSASFLRADRVSLSVGGGVGMGVPMTMPVRRLTIQMAHK 63

Query: 61  KGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 120
           KGAGSTKNGRDS GQRLGVKI+GDQVAKPG+II+RQRGT+ + G NVG+GKDHT+FSLID
Sbjct: 64  KGAGSTKNGRDSPGQRLGVKIYGDQVAKPGAIIIRQRGTRVYPGNNVGMGKDHTLFSLID 123

Query: 121 GLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVL 180
           GLVKFEKYGPDKKKVSVYP E QPENPNSYRARKREYFR+QRERKKAR EG IV+ QLVL
Sbjct: 124 GLVKFEKYGPDKKKVSVYPYEKQPENPNSYRARKREYFRMQRERKKARAEG-IVEVQLVL 183

Query: 181 ASTD-ATETNPVC 184
           A+ D + E N  C
Sbjct: 184 AAADESPEVNADC 195

[Close]

BLAST of Lsi03G003970 vs. ExPASy Swiss-Prot
Match: A6Q4D3 (50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=rpmA PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.4e-29
Identity = 60/84 (71.43%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           AHKKG GST+N RDS G+RLGVK FG +  + G+II+RQRGTK H G NVGLGKDHTIF+
Sbjct: 2   AHKKGQGSTQNNRDSAGRRLGVKKFGGEFVRAGNIIIRQRGTKVHPGNNVGLGKDHTIFA 61

Query: 109 LIDGLVKFEKYGPDKKKVSVYPRE 133
           LIDG+VKFE+YG D++KVSVYP E
Sbjct: 62  LIDGVVKFERYGKDRQKVSVYPVE 85

[Close]

BLAST of Lsi03G003970 vs. ExPASy TrEMBL
Match: A0A6J1K388 (50S ribosomal protein L27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489672 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 2.8e-89
Identity = 175/185 (94.59%), Postives = 177/185 (95.68%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLA--SSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNL+ AF GLSLA  SSSSFFKGDFGSIQ  PKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 60

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSISQPQLVLASTDATE 180

Query: 181 TNPVC 184
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Lsi03G003970 vs. ExPASy TrEMBL
Match: A0A6J1FG23 (50S ribosomal protein L27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445091 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.3e-89
Identity = 174/185 (94.05%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLA--SSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNL+ AF GLSLA  SSSSFFKGDFGSIQ  PKLSVSFP RFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLAPSSSSSFFKGDFGSIQLPPKLSVSFPTRFPLTIENAHKKGAGSTK 60

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 180

Query: 181 TNPVC 184
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Lsi03G003970 vs. ExPASy TrEMBL
Match: A0A0A0LQ66 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G406670 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.3e-89
Identity = 171/183 (93.44%), Postives = 176/183 (96.17%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNG 60
           M+LNL+++F GLSLASSSSFFKGDFGSI  SPKLSVSFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGII QPQL+LASTD  ETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of Lsi03G003970 vs. ExPASy TrEMBL
Match: A0A1S3B381 (50S ribosomal protein L27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485482 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.4e-88
Identity = 170/183 (92.90%), Postives = 174/183 (95.08%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNG 60
           M+LNLVA+F GLSLASSSSFFKGDFGSI   P LS+SFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLVASFRGLSLASSSSFFKGDFGSIHTPPNLSLSFPNTFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGII QPQL+LASTD  ETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLILASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of Lsi03G003970 vs. ExPASy TrEMBL
Match: A0A6J1BXH1 (50S ribosomal protein L27, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006224 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.3e-88
Identity = 170/185 (91.89%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSL--ASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNLV AF GLSL  +SSSSFF+GDF S+QP PK S+SFPNRFPLTIENAHKKGAGSTK
Sbjct: 6   MSLNLVGAFRGLSLVSSSSSSFFRGDFASVQPPPKFSLSFPNRFPLTIENAHKKGAGSTK 65

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKIFGDQ+AKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 66  NGRDSKGQRLGVKIFGDQLAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 125

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLAS+DATE
Sbjct: 126 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSIAQPQLVLASSDATE 185

Query: 181 TNPVC 184
           TNPVC
Sbjct: 186 TNPVC 190

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: XP_038884234.1 (50S ribosomal protein L27, chloroplastic [Benincasa hispida])

HSP 1 Score: 341.3 bits (874), Expect = 5.3e-90
Identity = 175/183 (95.63%), Postives = 175/183 (95.63%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNG 60
           MSLNLVAAF GLSL SSSSFFKGDFGSIQ  PKLS SFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MSLNLVAAFKGLSLVSSSSFFKGDFGSIQTPPKLSFSFPNTFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGII QP LVLASTDATETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPLLVLASTDATETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: XP_023550670.1 (50S ribosomal protein L27, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023550671.1 50S ribosomal protein L27, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6578368.1 50S ribosomal protein L27, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 339.0 bits (868), Expect = 2.6e-89
Identity = 175/185 (94.59%), Postives = 177/185 (95.68%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLA--SSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNL+ AF GLSLA  SSSSFFKGDFGSIQ  PKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 60

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 180

Query: 181 TNPVC 184
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: KAG7015950.1 (50S ribosomal protein L27, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 339.0 bits (868), Expect = 2.6e-89
Identity = 175/185 (94.59%), Postives = 177/185 (95.68%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLA--SSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNL+ AF GLSLA  SSSSFFKGDFGSIQ  PKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 20  MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 79

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 80  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 139

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLASTDATE
Sbjct: 140 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 199

Query: 181 TNPVC 184
           TNPVC
Sbjct: 200 TNPVC 204

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: XP_022993762.1 (50S ribosomal protein L27, chloroplastic [Cucurbita maxima] >XP_022993763.1 50S ribosomal protein L27, chloroplastic [Cucurbita maxima])

HSP 1 Score: 337.8 bits (865), Expect = 5.8e-89
Identity = 175/185 (94.59%), Postives = 177/185 (95.68%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLA--SSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNL+ AF GLSLA  SSSSFFKGDFGSIQ  PKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 60

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSISQPQLVLASTDATE 180

Query: 181 TNPVC 184
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: XP_022939083.1 (50S ribosomal protein L27, chloroplastic [Cucurbita moschata] >XP_022939084.1 50S ribosomal protein L27, chloroplastic [Cucurbita moschata] >XP_022939085.1 50S ribosomal protein L27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 336.7 bits (862), Expect = 1.3e-88
Identity = 174/185 (94.05%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLA--SSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTK 60
           MSLNL+ AF GLSLA  SSSSFFKGDFGSIQ  PKLSVSFP RFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLAPSSSSSFFKGDFGSIQLPPKLSVSFPTRFPLTIENAHKKGAGSTK 60

Query: 61  NGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120
           NGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATE 180
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG I QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 180

Query: 181 TNPVC 184
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Lsi03G003970 vs. TAIR 10
Match: AT5G40950.1 (ribosomal protein large subunit 27 )

HSP 1 Score: 258.5 bits (659), Expect = 4.2e-69
Identity = 138/193 (71.50%), Postives = 156/193 (80.83%), Query Frame = 0


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNR-----FPLTIENAHKKGAG 60
           MSLNL+ AF GLSL+S+SSF +GD  S  P    +V+ P        PLTIE+AHKKGAG
Sbjct: 7   MSLNLIGAFKGLSLSSTSSFLRGDL-SFSPKTSFTVTLPLENLQAPIPLTIESAHKKGAG 66

Query: 61  STKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVK 120
           STKNGRDS GQRLGVKI+GDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLIDGLVK
Sbjct: 67  STKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLIDGLVK 126

Query: 121 FEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---IIVQPQLVL- 180
           FEK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E     +  P+LVL 
Sbjct: 127 FEKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTPELVLA 186

Query: 181 -ASTDATETNPVC 184
            AS D  E NP C
Sbjct: 187 SASVDDAEANPEC 198

[Close]

BLAST of Lsi03G003970 vs. TAIR 10
Match: AT2G16930.2 (Ribosomal protein L27 family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Lsi03G003970 vs. TAIR 10
Match: AT2G16930.1 (Ribosomal protein L27 family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Lsi03G003970 vs. TAIR 10
Match: AT2G16930.3 (Ribosomal protein L27 family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Lsi03G003970 vs. TAIR 10
Match: AT5G15220.1 (Ribosomal protein L27 family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

The following BLAST results are available for this feature:

BLAST of Lsi03G003970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30155	1.8e-72	83.83	50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL27 P...	[more]
P82190	2.5e-71	76.06	50S ribosomal protein L27, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL27 P...	[more]
Q9FLN4	5.9e-68	71.50	50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL2...	[more]
O65037	2.0e-60	66.84	50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
A6Q4D3	5.4e-29	71.43	50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=rpm...	[more]

BLAST of Lsi03G003970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K388	2.8e-89	94.59	50S ribosomal protein L27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1FG23	6.3e-89	94.05	50S ribosomal protein L27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A0A0LQ66	6.3e-89	93.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G406670 PE=3 SV=1	[more]
A0A1S3B381	1.4e-88	92.90	50S ribosomal protein L27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485482...	[more]
A0A6J1BXH1	5.3e-88	91.89	50S ribosomal protein L27, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of Lsi03G003970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884234.1	5.3e-90	95.63	50S ribosomal protein L27, chloroplastic [Benincasa hispida]	[more]
XP_023550670.1	2.6e-89	94.59	50S ribosomal protein L27, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023550...	[more]
KAG7015950.1	2.6e-89	94.59	50S ribosomal protein L27, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022993762.1	5.8e-89	94.59	50S ribosomal protein L27, chloroplastic [Cucurbita maxima] >XP_022993763.1 50S ...	[more]
XP_022939083.1	1.3e-88	94.05	50S ribosomal protein L27, chloroplastic [Cucurbita moschata] >XP_022939084.1 50...	[more]

BLAST of Lsi03G003970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G40950.1	4.2e-69	71.50	ribosomal protein large subunit 27	[more]
AT2G16930.2	1.8e-19	65.71	Ribosomal protein L27 family protein	[more]
AT2G16930.1	1.8e-19	65.71	Ribosomal protein L27 family protein	[more]
AT2G16930.3	1.8e-19	65.71	Ribosomal protein L27 family protein	[more]
AT5G15220.1	1.8e-19	65.71	Ribosomal protein L27 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 141..161
None	No IPR available	GENE3D	2.40.50.100		coord: 53..166 e-value: 2.0E-29 score: 103.8
None	No IPR available	PANTHER	PTHR15893:SF0	39S RIBOSOMAL PROTEIN L27, MITOCHONDRIAL	coord: 34..176
None	No IPR available	SUPERFAMILY	110324	Ribosomal L27 protein-like	coord: 49..130
IPR001684	Ribosomal protein L27	PRINTS	PR00063	RIBOSOMALL27	coord: 51..75 score: 66.77 coord: 76..100 score: 62.92 coord: 101..125 score: 49.23
IPR001684	Ribosomal protein L27	TIGRFAM	TIGR00062	TIGR00062	coord: 48..128 e-value: 1.1E-36 score: 122.7
IPR001684	Ribosomal protein L27	PFAM	PF01016	Ribosomal_L27	coord: 49..128 e-value: 4.3E-36 score: 122.8
IPR001684	Ribosomal protein L27	PANTHER	PTHR15893	RIBOSOMAL PROTEIN L27	coord: 34..176
IPR001684	Ribosomal protein L27	HAMAP	MF_00539	Ribosomal_L27	coord: 48..131 score: 27.282888
IPR018261	Ribosomal protein L27, conserved site	PROSITE	PS00831	RIBOSOMAL_L27	coord: 81..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G003970.1	Lsi03G003970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005840	ribosome
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0003735	structural constituent of ribosome