Lsi02G019710 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G019710 vs. ExPASy TrEMBL
Match: A0A6J1CPW6 (uncharacterized protein LOC111013495 OS=Momordica charantia OX=3673 GN=LOC111013495 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 8.3e-119
Identity = 285/477 (59.75%), Postives = 342/477 (71.70%), Query Frame = 0

Query: 1   MGSDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVIT 60
           MG DFELKISP+      S FELQL +GT +P F+SNETNSMFILTAKLTGF+ +DI IT
Sbjct: 34  MGLDFELKISPS---SSFSNFELQLARGTPSP-FWSNETNSMFILTAKLTGFSREDIGIT 93

Query: 61  INGSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 120
           ING+ T+IEIGWG+PV++MEIT   YKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI
Sbjct: 94  INGTGTRIEIGWGQPVQRMEITRWMYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 153

Query: 121 LKISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL---LETLETGRKTNLVDENH 180
           LKI MPK VDG+LGVGIEEVKEQ+ GE   ESQD E EE+    E  ETGR+T  V    
Sbjct: 154 LKIVMPKLVDGILGVGIEEVKEQDVGEDALESQDSEAEEIPEVTEGFETGRETKFV---- 213

Query: 181 EKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSERK 240
               +DA  G  E  NA PE   K EY+AE   EE EKLKG EM+  IDG S + YSE +
Sbjct: 214 ----EDAANGVFEGANATPEPSPKREYLAETTQEETEKLKGDEMEH-IDGVS-NHYSESE 273

Query: 241 QTVKVATKTTEYEATREN---VNGEIKAPKVRSKEGIEVAEGTT----NHQKEK-RLDAE 300
           ++ K+ T+  E     +N   V+ EI  PKVRS +GIE+ EGT+    NHQKEK   ++E
Sbjct: 274 KSEKLTTEIAEAPKRWDNGGIVDSEIIIPKVRSVKGIELDEGTSKGTANHQKEKGEPNSE 333

Query: 301 SSNSDKA-------NVKMQSQASIDREAQVLD-CELLQPNRQTRAE------SGKQETYK 360
           + N   A       ++K+QSQA++DR+ +V D  EL QPN + +AE       G +ETY+
Sbjct: 334 AHNGSIATMTNKQPDIKIQSQAAVDRDTKVPDKPELSQPNGKIQAEHKIVEQQGNKETYR 393

Query: 361 NVQELKELETDEEPEDIGRPYLQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREEC----K 420
           +  E KE ET+ EPE I  PYLQSP H+HEEKGV +++G+SFQT+DEIK+ REE     +
Sbjct: 394 SAWESKEFETNNEPEYIASPYLQSPEHEHEEKGV-NQQGKSFQTQDEIKEAREESGSPQR 453

Query: 421 STHEDSSNEAKQEKKQDSKQFKMRTPIIIAGSALLASLAIIVFNLVKSKRREKNMRG 449
             HE S+ EAKQEK QDSKQFKMRTPIIIAGSALLASLAI+VFN V+SK+REKNMRG
Sbjct: 454 EKHEQSTKEAKQEKIQDSKQFKMRTPIIIAGSALLASLAIVVFNFVRSKKREKNMRG 495

BLAST of Lsi02G019710 vs. ExPASy TrEMBL
Match: A0A6J1EKH3 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111433395 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 5.4e-110
Identity = 254/408 (62.25%), Postives = 297/408 (72.79%), Query Frame = 0

Query: 3   SDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVITIN 62
           S FELKISPTR+ DPSSTFEL L K T+T  F+S+ETNSMF+LTAKL GFA DDIVITIN
Sbjct: 5   SHFELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITIN 64

Query: 63  GSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILK 122
           GS T+IEI WG+PV+KMEIT  TYKKETETRRFKKIFRIPDGV+LDEIKATYNDE+SILK
Sbjct: 65  GSGTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILK 124

Query: 123 ISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL------LETLETGRKTNLVDEN 182
           I MPK  +G+LGVGI+EVKE+EAGEATFESQD E E +      LET ETG++T L+++ 
Sbjct: 125 ILMPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDT 184

Query: 183 HEKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER 242
           H         GE   ENA+ EAF K+EY AE  PEEIEKLKG+EM +D DG S +DYSE 
Sbjct: 185 HGN-------GEFGGENAKREAFSKMEYAAETTPEEIEKLKGNEM-EDTDGGS-NDYSEC 244

Query: 243 KQTVKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAEGTT----NHQKEK-RLDAESS 302
           +Q    ATK TEYE T+EN+NG+IK PKV S EGIEVAEGT+    NHQ+EK    AE  
Sbjct: 245 EQLETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQEEKGEGKAEEH 304

Query: 303 NSD-------KANVKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELE 362
           N D       K +VK Q QASID+EAQ L+ E  + NR TRAE GK ETY+N +ELK   
Sbjct: 305 NGDIATMIKKKLDVKTQPQASIDQEAQTLEQENFKRNRHTRAELGKLETYRNTRELK--- 364

Query: 363 TDEEPEDIGRPYLQSPAHQHEEKGV-DHKKGRSFQTKDEIKDTREECK 392
              EPEDI  PYLQSPAH+HEEK + D KK        ++K  R+E +
Sbjct: 365 ---EPEDIEIPYLQSPAHEHEEKEISDEKKTTQSGHTTKVKTGRDEVR 397

BLAST of Lsi02G019710 vs. ExPASy TrEMBL
Match: A0A6J1J0B1 (uncharacterized protein LOC111480753 OS=Cucurbita maxima OX=3661 GN=LOC111480753 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 7.1e-110
Identity = 249/382 (65.18%), Postives = 282/382 (73.82%), Query Frame = 0

Query: 3   SDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVITIN 62
           S FELKISPTR+ DPSSTFEL L K T+T  F+S+ETNSMF+LTAKL GFA DDIVITIN
Sbjct: 5   SHFELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITIN 64

Query: 63  GSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILK 122
           GS TQIEI WG+PV+KMEIT  TYKKETETRRFKKIFRIPDGV+LDEIKATYNDE+SILK
Sbjct: 65  GSGTQIEISWGQPVQKMEITRLTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILK 124

Query: 123 ISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL------LETLETGRKTNLVDEN 182
           I MPK V+G+LGVGIE VKEQEAG+ATFESQD E E +      LET ETG+KT LV++ 
Sbjct: 125 ILMPKLVEGILGVGIEGVKEQEAGDATFESQDSEAEAIPHAPTDLETPETGKKTKLVEDT 184

Query: 183 HEKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER 242
           H         GE   ENA+ EAF KIEY AE  PEEIEKLKG+EM +D DG S +DYSE 
Sbjct: 185 HGN-------GEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEM-EDTDGGS-NDYSES 244

Query: 243 KQTVKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAEGTT----NHQKEKRLDAESSN 302
           +Q    ATK TEYE T+EN+NG+I+ PKV S EGIEVAEGT+    NHQKEK  +AE  N
Sbjct: 245 EQLETGATKATEYEGTKENINGQIQVPKVTSMEGIEVAEGTSQETANHQKEKEGNAEEHN 304

Query: 303 SD-------KANVKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELET 362
            D       K +VK Q QASID+E      E  + NR TRA  GK ETY+N +ELK    
Sbjct: 305 GDMATMIKKKPDVKTQPQASIDQEQ-----ENFKQNRHTRAGLGKPETYRNTRELK---- 364

Query: 363 DEEPEDIGRPYLQSPAHQHEEK 368
             EPEDI  PYLQSPAH+HEEK
Sbjct: 365 --EPEDIEIPYLQSPAHEHEEK 366

BLAST of Lsi02G019710 vs. ExPASy TrEMBL
Match: A0A5A7UWU5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold518G00820 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.7e-55
Identity = 191/460 (41.52%), Postives = 239/460 (51.96%), Query Frame = 0

Query: 1   MGSDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVIT 60
           MGSDFELKI PT D D SSTFELQL +G+AT             L   +  FA       
Sbjct: 49  MGSDFELKIKPTIDDDTSSTFELQLAEGSAT-------------LMKPIPCFA------- 108

Query: 61  INGSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 120
                T +    G  VRK ++T                 ++  GV   E+       + +
Sbjct: 109 -----TLVRNVIGTLVRKSDVTLPPQ-------------QLAYGVWFSEVL------KYL 168

Query: 121 LKISMPKSVDGMLGVGIEEVKE-QEAGEATFESQDYEEEELLETLETGRKTNLVDENHEK 180
             +SM          G+E +K+  +   +     +  +   L   +   +   +    ++
Sbjct: 169 TMLSMSTH------FGVEPIKDSDQVWPSEVPRSNDRDRSCLRLWKLAERPTWLTVIMKR 228

Query: 181 EEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSERKQT 240
            +      +++++NAE E  HKIEYVAE+ P+EIEKLKGSEMD+DID             
Sbjct: 229 RKPMLLMEQLKKKNAETERLHKIEYVAEIQPDEIEKLKGSEMDEDID------------- 288

Query: 241 VKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAE----GTTNHQKEKRLDAESSNSDK 300
                                  PK+RS EGIEVAE    GT +H+KE + +AE+ N DK
Sbjct: 289 ----------------------VPKIRSVEGIEVAEVTSKGTASHKKETQTNAEARNGDK 348

Query: 301 AN-------VKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELETDEE 360
           A        VK QSQASIDREA                           +ELKELETD+E
Sbjct: 349 ATMINKKPYVKTQSQASIDREA---------------------------RELKELETDKE 394

Query: 361 PEDIGRPYLQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREECKSTHEDSSNEAKQEKKQD 420
           PEDIG PYLQ PA+QH+EKG D +KGRSFQTK E+KD REECKSTHEDSS EAKQEKKQD
Sbjct: 409 PEDIGSPYLQRPANQHDEKGAD-RKGRSFQTKYEVKDAREECKSTHEDSSKEAKQEKKQD 394

Query: 421 SKQFKMRTPIIIAGSALLASLAIIVFNLVKSKRREKNMRG 449
           SKQ KMRT IIIAGSALLASL  + FNLVKSK  EKN++G
Sbjct: 469 SKQLKMRTSIIIAGSALLASLVTVAFNLVKSK-TEKNVKG 394

BLAST of Lsi02G019710 vs. ExPASy TrEMBL
Match: A0A5D3CSD6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1081G00020 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.1e-53
Identity = 149/272 (54.78%), Postives = 172/272 (63.24%), Query Frame = 0

Query: 188 EVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSERKQTVKVATKTT 247
           +++++NAE E  HKIEYVAE+ P+EIEKLKGSEMD+DID                     
Sbjct: 33  QLKKKNAETERLHKIEYVAEIQPDEIEKLKGSEMDEDID--------------------- 92

Query: 248 EYEATRENVNGEIKAPKVRSKEGIEVAE----GTTNHQKEKRLDAESSNSDKAN------ 307
                          PK+RS EGIEVAE    GT +H+KE + +AE+ N DKA       
Sbjct: 93  --------------VPKIRSVEGIEVAEVTSKGTASHKKETQTNAEARNGDKATMINKKP 152

Query: 308 -VKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELETDEEPEDIGRPY 367
            VK QSQASIDREA                           +ELKELETD+EPEDIG PY
Sbjct: 153 YVKTQSQASIDREA---------------------------RELKELETDKEPEDIGSPY 212

Query: 368 LQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREECKSTHEDSSNEAKQEKKQDSKQFKMRT 427
           LQ PA+QH+EKG D +KGRSFQTK E+KD REECKSTHEDSS EAKQEKKQDSKQ KMRT
Sbjct: 213 LQRPANQHDEKGAD-RKGRSFQTKYEVKDAREECKSTHEDSSKEAKQEKKQDSKQLKMRT 240

Query: 428 PIIIAGSALLASLAIIVFNLVKSKRREKNMRG 449
            IIIAGSALLASL  + FNLVKSK  EKN++G
Sbjct: 273 SIIIAGSALLASLVTVAFNLVKSK-TEKNVKG 240

BLAST of Lsi02G019710 vs. NCBI nr
Match: XP_023521223.1 (neurofilament medium polypeptide-like [Cucurbita pepo subsp. pepo] >XP_023522087.1 neurofilament medium polypeptide-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-136
Identity = 306/467 (65.52%), Postives = 352/467 (75.37%), Query Frame = 0

Query: 3   SDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVITIN 62
           S FELKISPTR+ DPSSTFEL L K T+T  F+S+ETNSMF+LTAKL GFA DDIVITIN
Sbjct: 5   SHFELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITIN 64

Query: 63  GSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILK 122
           GS T+IEI WG+PV+KMEIT  TYKKETETRRFKKIFRIPDGV+LDEIKATYNDE+SILK
Sbjct: 65  GSGTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILK 124

Query: 123 ISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL------LETLETGRKTNLVDEN 182
           I MPK  +G+LGVGI+EVKEQEAG+ATFESQD E E +      LET ETG++T L+++ 
Sbjct: 125 ILMPKLEEGILGVGIQEVKEQEAGKATFESQDSEAEAIPHTPTDLETPETGKETKLIEDT 184

Query: 183 HEKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER 242
           H         GE   ENA+ EAF KIEY AE  PEEIEKLKG+EM +D DG S +DYSE 
Sbjct: 185 HGN-------GEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEM-EDTDGGS-NDYSES 244

Query: 243 KQTVKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAEGTT----NHQKEKRLDAESSN 302
           +Q    ATK TEYE T+EN+NG+IK PKV S EGIEVAEGT+    NHQKEK  +A+  N
Sbjct: 245 EQLETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQKEKEGNAKEHN 304

Query: 303 SD-------KANVKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELET 362
            D       K +VK QSQASID+E      E  + NR TRAE GK ETY+N +ELK    
Sbjct: 305 GDMATMIKKKPDVKTQSQASIDQEQ-----ENFKRNRHTRAELGKPETYRNTRELK---- 364

Query: 363 DEEPEDIGRPYLQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREECKS----THEDSSNEA 422
             EPEDI  PYLQSPAH+HEEK VD +KG+S QTKDE K+ REEC S    TH+DSS EA
Sbjct: 365 --EPEDIEIPYLQSPAHEHEEKEVD-QKGKSSQTKDETKEAREECNSPQKVTHQDSSKEA 424

Query: 423 KQEKKQDSKQFKMRTPIIIAGSALLASLAIIVFNLVKSKRREKNMRG 449
           KQEK    KQFKMR PII+AGSAL+ASL I+VF+LVKSK+REKNMRG
Sbjct: 425 KQEK----KQFKMRIPIIVAGSALVASLVIVVFSLVKSKKREKNMRG 446

BLAST of Lsi02G019710 vs. NCBI nr
Match: KAG6607636.1 (hypothetical protein SDJN03_00978, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037240.1 hypothetical protein SDJN02_00863, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 489.2 bits (1258), Expect = 3.8e-134
Identity = 300/467 (64.24%), Postives = 349/467 (74.73%), Query Frame = 0

Query: 3   SDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVITIN 62
           S FELKISPTR+ DPSSTFEL L K T+T  F+S+ETNSMF+LTAKL GFA DDIV+TIN
Sbjct: 5   SHFELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVVTIN 64

Query: 63  GSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILK 122
           GS T+IEI WG+PV+KMEIT  TYKKETETRRFKKIFRIPDGV+LDEIKATYNDE+SILK
Sbjct: 65  GSGTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILK 124

Query: 123 ISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL------LETLETGRKTNLVDEN 182
           I MPK  +G+LGVGI+EVKE+EAGEATFESQD E E +      LET ETG++T L+++ 
Sbjct: 125 ILMPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDT 184

Query: 183 HEKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER 242
           H         GE+  ENA+ EAF K+EY AE  PEEIEKLKG+EM +D DG S +DYSE 
Sbjct: 185 HGN-------GELGGENAKREAFSKMEYAAETTPEEIEKLKGNEM-EDTDGGS-NDYSES 244

Query: 243 KQTVKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAEGTT----NHQKEKRLDAESSN 302
           ++    ATK TEYE T+EN+NG+IK PKV   EGIEVAEGT+    NHQKEK  +AE  N
Sbjct: 245 ERLETGATKATEYEGTKENINGQIKVPKVTITEGIEVAEGTSQETANHQKEKEGNAEEHN 304

Query: 303 SD-------KANVKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELET 362
            D       K +VK Q QASID+E      E  + NR TR E GK ETY+N  +LK    
Sbjct: 305 GDMATMIKKKPDVKTQPQASIDQEQ-----ENFKRNRHTRVELGKPETYRNTWKLK---- 364

Query: 363 DEEPEDIGRPYLQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREECKS----THEDSSNEA 422
             EPEDI  PYLQSPAH+HEEK VD +KG+SFQTKDE K+ REEC S    TH+DSS EA
Sbjct: 365 --EPEDIEIPYLQSPAHEHEEKEVD-QKGKSFQTKDETKEAREECNSPQKVTHQDSSKEA 424

Query: 423 KQEKKQDSKQFKMRTPIIIAGSALLASLAIIVFNLVKSKRREKNMRG 449
           KQEK    KQFKMR PII+AGSAL+A+L I VF+LVKSK+REKNMRG
Sbjct: 425 KQEK----KQFKMRIPIIVAGSALVATLVIAVFSLVKSKKREKNMRG 446

BLAST of Lsi02G019710 vs. NCBI nr
Match: XP_022143634.1 (uncharacterized protein LOC111013495 [Momordica charantia])

HSP 1 Score: 437.2 bits (1123), Expect = 1.7e-118
Identity = 285/477 (59.75%), Postives = 342/477 (71.70%), Query Frame = 0

Query: 1   MGSDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVIT 60
           MG DFELKISP+      S FELQL +GT +P F+SNETNSMFILTAKLTGF+ +DI IT
Sbjct: 34  MGLDFELKISPS---SSFSNFELQLARGTPSP-FWSNETNSMFILTAKLTGFSREDIGIT 93

Query: 61  INGSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 120
           ING+ T+IEIGWG+PV++MEIT   YKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI
Sbjct: 94  INGTGTRIEIGWGQPVQRMEITRWMYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 153

Query: 121 LKISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL---LETLETGRKTNLVDENH 180
           LKI MPK VDG+LGVGIEEVKEQ+ GE   ESQD E EE+    E  ETGR+T  V    
Sbjct: 154 LKIVMPKLVDGILGVGIEEVKEQDVGEDALESQDSEAEEIPEVTEGFETGRETKFV---- 213

Query: 181 EKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSERK 240
               +DA  G  E  NA PE   K EY+AE   EE EKLKG EM+  IDG S + YSE +
Sbjct: 214 ----EDAANGVFEGANATPEPSPKREYLAETTQEETEKLKGDEMEH-IDGVS-NHYSESE 273

Query: 241 QTVKVATKTTEYEATREN---VNGEIKAPKVRSKEGIEVAEGTT----NHQKEK-RLDAE 300
           ++ K+ T+  E     +N   V+ EI  PKVRS +GIE+ EGT+    NHQKEK   ++E
Sbjct: 274 KSEKLTTEIAEAPKRWDNGGIVDSEIIIPKVRSVKGIELDEGTSKGTANHQKEKGEPNSE 333

Query: 301 SSNSDKA-------NVKMQSQASIDREAQVLD-CELLQPNRQTRAE------SGKQETYK 360
           + N   A       ++K+QSQA++DR+ +V D  EL QPN + +AE       G +ETY+
Sbjct: 334 AHNGSIATMTNKQPDIKIQSQAAVDRDTKVPDKPELSQPNGKIQAEHKIVEQQGNKETYR 393

Query: 361 NVQELKELETDEEPEDIGRPYLQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREEC----K 420
           +  E KE ET+ EPE I  PYLQSP H+HEEKGV +++G+SFQT+DEIK+ REE     +
Sbjct: 394 SAWESKEFETNNEPEYIASPYLQSPEHEHEEKGV-NQQGKSFQTQDEIKEAREESGSPQR 453

Query: 421 STHEDSSNEAKQEKKQDSKQFKMRTPIIIAGSALLASLAIIVFNLVKSKRREKNMRG 449
             HE S+ EAKQEK QDSKQFKMRTPIIIAGSALLASLAI+VFN V+SK+REKNMRG
Sbjct: 454 EKHEQSTKEAKQEKIQDSKQFKMRTPIIIAGSALLASLAIVVFNFVRSKKREKNMRG 495

BLAST of Lsi02G019710 vs. NCBI nr
Match: XP_022926215.1 (neurofilament medium polypeptide-like [Cucurbita moschata])

HSP 1 Score: 407.9 bits (1047), Expect = 1.1e-109
Identity = 254/408 (62.25%), Postives = 297/408 (72.79%), Query Frame = 0

Query: 3   SDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVITIN 62
           S FELKISPTR+ DPSSTFEL L K T+T  F+S+ETNSMF+LTAKL GFA DDIVITIN
Sbjct: 5   SHFELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITIN 64

Query: 63  GSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILK 122
           GS T+IEI WG+PV+KMEIT  TYKKETETRRFKKIFRIPDGV+LDEIKATYNDE+SILK
Sbjct: 65  GSGTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILK 124

Query: 123 ISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL------LETLETGRKTNLVDEN 182
           I MPK  +G+LGVGI+EVKE+EAGEATFESQD E E +      LET ETG++T L+++ 
Sbjct: 125 ILMPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDT 184

Query: 183 HEKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER 242
           H         GE   ENA+ EAF K+EY AE  PEEIEKLKG+EM +D DG S +DYSE 
Sbjct: 185 HGN-------GEFGGENAKREAFSKMEYAAETTPEEIEKLKGNEM-EDTDGGS-NDYSEC 244

Query: 243 KQTVKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAEGTT----NHQKEK-RLDAESS 302
           +Q    ATK TEYE T+EN+NG+IK PKV S EGIEVAEGT+    NHQ+EK    AE  
Sbjct: 245 EQLETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQEEKGEGKAEEH 304

Query: 303 NSD-------KANVKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELE 362
           N D       K +VK Q QASID+EAQ L+ E  + NR TRAE GK ETY+N +ELK   
Sbjct: 305 NGDIATMIKKKLDVKTQPQASIDQEAQTLEQENFKRNRHTRAELGKLETYRNTRELK--- 364

Query: 363 TDEEPEDIGRPYLQSPAHQHEEKGV-DHKKGRSFQTKDEIKDTREECK 392
              EPEDI  PYLQSPAH+HEEK + D KK        ++K  R+E +
Sbjct: 365 ---EPEDIEIPYLQSPAHEHEEKEISDEKKTTQSGHTTKVKTGRDEVR 397

BLAST of Lsi02G019710 vs. NCBI nr
Match: XP_022981685.1 (uncharacterized protein LOC111480753 [Cucurbita maxima])

HSP 1 Score: 407.5 bits (1046), Expect = 1.5e-109
Identity = 249/382 (65.18%), Postives = 282/382 (73.82%), Query Frame = 0

Query: 3   SDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVITIN 62
           S FELKISPTR+ DPSSTFEL L K T+T  F+S+ETNSMF+LTAKL GFA DDIVITIN
Sbjct: 5   SHFELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITIN 64

Query: 63  GSSTQIEIGWGKPVRKMEITGRTYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILK 122
           GS TQIEI WG+PV+KMEIT  TYKKETETRRFKKIFRIPDGV+LDEIKATYNDE+SILK
Sbjct: 65  GSGTQIEISWGQPVQKMEITRLTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILK 124

Query: 123 ISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEEL------LETLETGRKTNLVDEN 182
           I MPK V+G+LGVGIE VKEQEAG+ATFESQD E E +      LET ETG+KT LV++ 
Sbjct: 125 ILMPKLVEGILGVGIEGVKEQEAGDATFESQDSEAEAIPHAPTDLETPETGKKTKLVEDT 184

Query: 183 HEKEEDDADYGEVEEENAEPEAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER 242
           H         GE   ENA+ EAF KIEY AE  PEEIEKLKG+EM +D DG S +DYSE 
Sbjct: 185 HGN-------GEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEM-EDTDGGS-NDYSES 244

Query: 243 KQTVKVATKTTEYEATRENVNGEIKAPKVRSKEGIEVAEGTT----NHQKEKRLDAESSN 302
           +Q    ATK TEYE T+EN+NG+I+ PKV S EGIEVAEGT+    NHQKEK  +AE  N
Sbjct: 245 EQLETGATKATEYEGTKENINGQIQVPKVTSMEGIEVAEGTSQETANHQKEKEGNAEEHN 304

Query: 303 SD-------KANVKMQSQASIDREAQVLDCELLQPNRQTRAESGKQETYKNVQELKELET 362
            D       K +VK Q QASID+E      E  + NR TRA  GK ETY+N +ELK    
Sbjct: 305 GDMATMIKKKPDVKTQPQASIDQEQ-----ENFKQNRHTRAGLGKPETYRNTRELK---- 364

Query: 363 DEEPEDIGRPYLQSPAHQHEEK 368
             EPEDI  PYLQSPAH+HEEK
Sbjct: 365 --EPEDIEIPYLQSPAHEHEEK 366

BLAST of Lsi02G019710 vs. TAIR 10
Match: AT1G76770.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 6.1e-21
Identity = 80/217 (36.87%), Postives = 116/217 (53.46%), Query Frame = 0

Query: 1   MGSDFELKISPTRDGDPSSTFELQLTKGTATPFFYSNETNSMFILTAKLTGFAADDIVIT 60
           M  +  LKI+ T+D D SS+ + ++ + +      S ETNS+F L   L GF  D I I 
Sbjct: 1   MELELGLKITRTKD-DVSSSTDFRVARDSFGHRSLSRETNSVFFLILHLKGFKKDGIDIE 60

Query: 61  INGSSTQIEIGWGKPVRKMEITGRT-YKKETETRRFKKIFRIPDGVVLDEIKATYNDEES 120
           IN     I+I   K V +M +     +KKETE + FKK+FRIPD V+LD+IKA +N+E+ 
Sbjct: 61  INKEGNLIKISGRKQVEEMVLVKWVEWKKETEIKEFKKVFRIPDIVILDKIKARFNEEDG 120

Query: 121 ILKISMPKSVDGMLGVGIEEVKEQEAGEATFESQDYEEEELLETLETGRKTNLVDENHEK 180
            L ++MPK V G+ G+ IEE  E+E  +     +  EE+   E  E   +T   +EN E 
Sbjct: 121 TLTVTMPKKVKGITGLKIEEEDEEEEMKEPIVEEKTEEKTEPEE-EIKEETKPEEENEEA 180

Query: 181 EEDD-ADYGEVEEENAEPEAFHKIEYVAEMPPEEIEK 216
           EE    +  EV EE        K E + + P ++  K
Sbjct: 181 EEPQREEEEEVVEEGTRDHEGKKEEEIEDKPRKKRRK 215

BLAST of Lsi02G019710 vs. TAIR 10
Match: AT1G76780.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.5e-08
Identity = 99/345 (28.70%), Postives = 156/345 (45.22%), Query Frame = 0

Query: 86  YKKETETRRFKKIFRIPDGVVLDEIKATYNDEESILKISMPKSVDGMLGVGIEEVKEQEA 145
           ++KE E   FKK+FRIPD V LD+IKA ++D+++ L I+MPK V G+ G  IEE +E+E 
Sbjct: 9   WRKEVEFSVFKKVFRIPDTVDLDKIKARFDDDDATLTITMPKRVKGISGFKIEEEEEEEE 68

Query: 146 GEATFESQDYEEEELLETLETG-------RKTNLVDENHEKEEDDADYGE--VEEENAEP 205
            E   E  D  E E  E  E G        K++ +DE  E+E+  AD  +  V+    +P
Sbjct: 69  EEE--ERVDVSEAEHKEETEKGELKDDYLEKSHQIDERIEEEKGLADSNKESVDSSLRKP 128

Query: 206 EAFHKIEYVAEMPPEEIEKLKGSEMDQDIDGDSHDDYSER------KQTVKVATKTTEYE 265
                 E   +   EE E  K  ++ Q  + D  DD+  R      +Q   V  +T+   
Sbjct: 129 PDIEGRECHEQTRHEEQENNK--QLVQ-AESDDSDDFGSRAFEEIEEQESDVLDRTSTSG 188

Query: 266 ATRENVNGEIKAPKVRSKEGIEVAEGTTNHQK-EKRLDAESSNSDKANVKMQSQASIDRE 325
           A  + +  ++    +R  +GIE  E      K  + +D E+S  +K  V    + + D +
Sbjct: 189 AMEKEMTDDV-GDGLRKVQGIEEPERHNEESKISEMVDGETSGHEKKKVVKMDKKNRDVK 248

Query: 326 AQVLDC--ELLQPN---RQTRAESGKQETYKNVQELK--ELETDE--------------E 385
            +V     E  +PN    Q   +    ET KN +E +  +LE DE               
Sbjct: 249 EEVDGAMGEGFRPNIDRTQVVGDDEIAETEKNDEEFESDKLEADEVDKINEGGNTKVRRH 308

Query: 386 PEDIGRPYLQSPAHQHEEKGVDHKKGRSFQTKDEIKDTREECKST 394
            ED     LQ    QH ++   H K  +   K+ +++   E ++T
Sbjct: 309 SEDRNLIKLQEKEEQHSKEQKGHSKEENM--KELVEEKTPEAETT 345