Lsi02G018800 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G018800
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionIndole-3-acetate O-methyltransferase 1
Locationchr02: 24638290 .. 24643590 (+)
RNA-Seq ExpressionLsi02G018800
SyntenyLsi02G018800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTACACAAAAGCTTTCTCATAAAAGACTCTCCCCTTCTCCAAGCTTAAAGATAAAAGCTTCAAACCCTTCTCATGGCTCCCAAGGGCGACAACGTCGTCGTTTCGAACATGAAACTCGAACGAATGCTCAGCATGAAAGGAGGTAAAGGCGAAACGAGCTATGCTAACAACTCTCAAGCTCAGGTATTTTCTAACTTTTTTTTAACAAAAGTGATCGATTTTTTTAAAATGATCTATAACAAGAAAAAAAGAGAGATATTTTTAAGTATGTGACTTTGGAATCAAATTACGTATGCATCAGGTTTGTCAAAACATAACTAAAGAAAAAGGCTTTTGAAATTTTCGGATCAAGACTAAATAAATATGTATGATAAACTACATAATTCTTGATGTTTTACTTAGAAAATAATTTATATTTCTAAACACTTATTTTCTGAAAGATGAAATCTCTTTCATGAATTTTTTTCGATGAATACTTAATACATCCTGATTTGCACCTATCTTTGTATAGCCTACTCAATTGTCTAATCATCCTTTGTCACTAGACCAATTCTTCTTTTTTTTTTTTTTTTTTTTTAATAATTTTGACTTGGAGTGGGTTGCGTAAAGATAAGGAGAAGTTCGGGATGCACTCAAATTTTACGAGTCACTCAATCATGACACGATAAAACCGACATAATAATTATAGAGCTAAATTGGGTGTATGTAACTAAAATTTGAAAAAAAAAAACACGCAGGCTCAACATGCGAGATCAATGCTTCATCTTCTAAAAGAAACATTGGACGGAGTTCATTTAAACTCGCCGGAACTGCCGTTCGTGGTGGTGGATCTCGGCTGCAGCTGCGGCAGCAACACCATTTACATCGTCGACGTCATCATAAAGCACATTATAAAGCGCTTTGAGGCTTTGGCCGTCGACCCGCCGGAGTTCACCGCCTTTTTCTCCGATCTCCCTGGAAATGACTTCAATACACTCTTCCAGCTCCTCCCGCCTTTGGCCACTTATGGCGGCAGCATGGAGGAATGCCTCGCCGCCGACAACCATCGCTCCTACTTCGCTGCCGGCGTTCCTGGCTCCTTCTACCGCCGTCTGTTTCCGGCCAGGTCCATCGACGTTTTTCACTCCGCGTTTTCCTTGCATTGGCTCTCTCAGGTTTTTCCCATTATATTTTTCTTTGCCTTTTAAGTTCTAAAACTTTCGCTAAATTCCAATTATGGTCCTAAATTTTGAAAACTTTCATGTTGACCCCTTGAACTTTAACAAAATATCCTTGAAAGTCTTTTTTTTTTTTTTTTACTTTCTTTTGATTAAAATTATAAAGGTCGAAAATTGACGCTGATGTATAAAAATAAGAGGGTATAACTTTATGGTTGATTAAAATAGTGATTTTCATAAAATCGTGATTATTATCTTTTTTTTTGAAATGTTATTCACTTCATCTATAAAATCATTCACATAATCAATTTTTATACCTCTTATCAATAACTTTAAGAAAAAAAATTATTTTTTTAGTTCCTAAATTTTCGATCATAAGTACATTTAGTTTCCAAAATTTCATATAATTTACGGTTTTAGTCTCCAAAGTCTCATTGATTAATTTTTTTTAAGATAAACTCATTGATTAATTTTTTATCATTGATAATTTTAAAATATATATATATTTTTATCTCTCTCTTCCCTCCACCTTCTTTTTCTCTTCTTCTTTAATTTTTATTATAAATTTAAAAGGTCAAGTTAACATTTAACCTTCTTTTTCTCTTCTTCTTTAATTTTTATTATAAATTTAAAAGGTCAAGTTAACATTTAAAATAATTAAAAAAAGTTTTACAGGGAACCTTTTAAATATATTCTTAAAAACTCAAAAATTAAAAGAGTATATTTTAAAAGGTTAGGGATTGAACACACTAATTACTTTAGTGACAATAATCCTCTACAATAATTTCAACGATAAAAACTTTCCAACAAAATTTTTTCTTGAAACAATTTCAAAGTTGAAGGGCTCTCTTAAAAAAATGTTCACTAAAATGAAAATCTTTAAATTATCCCAAAGTTCATTGGTATTTTGTATATTTTAACTTTTTTTAACTTTTTATTATTTTGGTTATATATTTGCATGGCTATTACCTTGCAATTTTATTTTATTTCTTTCTTTCTTTCTTTATTATTATTATTATTATTATTATTATTTTTTTTGTAGATTTTGACTTTTCTTTCTGTTTTGGGTGGGGTTTAATTTTAATATTCCCGAGGAGTATTGAAGAAAGCTTTTTTAATTATTGGAATCTTTGGAATTTTGAGAAAGAGAAGAAAAAGGGATAATTTTTTTTTTCATATGTTTAACGATCTAAAAGGTTATGCATTGCATATAACGACCAATTCAATCAATTTACTTAGTTTTTTTTCCCTTTCTTGTTGATAACGTTTTTTATTAATATTTTTAAAATACTTAAATTTTATTATTGTGTTGTTATTAAACTTTCAAATAATCTATTCTTTTAGTTTGTATGTATATCTCTAAATTTTCTATAAATACTCATTTTTATTCTCAACATTATTTTTTTTATTTGGTTTGAAAAATGTACCTAGTTTATAACGATTATGTTTGGGAGTGGTTTTGACAAATTCGAACAAATAACAAAAAATACATTTAAACAAATCTTTAAAATATTTTTTTAGAATAAAAGACCATGACCAAAAACTACTCTTAAAACTATGGAATTCAACACACGTGTATCAGAGTGATAGCGCATGCTAATTAAATTATTTAAAAGCATACATATGCTTTTGTCTATATTTAGAGACGAATTTAATCTAAATTGAGTTTGTGGGACCCACCAACATGTAGGTACCAGAGACGGTGGTGGATGCGAGATCGACGGCATACAACAGAGGGAGGGTGTTCATCCACGGCGCAAACGAGGCGGCGGCGGCGGCTTACCGGAAGCAATTTCAGACGGACTTGGCCGGATTTCTGTGGGCCAGGGCCCACGAGCTGAAAAGAGGTGGGTCCATGTTCCTTGTCTGCTTGGGCCGGACCTCACTGGACCCCACCGACCAAGGTGGAGCCGGCCTCCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCTTGTCCAAGAGGTACAAAATATTGTTTTTTTATATATATTATTTAACAACTTTTTTTTTCTTCATTTTTGGAGTTTTGTATGAATTTTAAAGGTTTAGCTAAATGTCTAACTACTAATCTATTCTCACTCACTTTAAGGTTTATAAGAATTATTTTTAATTATTGAAACCTTTAAGCTTCTTTTCCTAGATTTGGTTTTTAGTCTTAATTTTTTGAAATTTATGTTTGTTTATTTTTAATTTCTCAGAAATACTTAAATTTCTAGAAAAGTTTTAAAAATAAAAACAAAATTTATGAATGAATGTAAGTAACATTTTTAAGCTTAATTTTTAAAATTTAAATAATTTCCAACCAGACATTAATTAAAACGACAAAAAAATAATAATAAAAGAGACATTTTTTTTTGCGTGTAAGAAATAAAAACCATAGACATAATAATAAGAACCGTCGATTAATTTTGTTTCATTCGGAAATATCTTACGTAAAAATGCATCTGAAAAATGCCCCCACCACCTGTTCAAATCTGTTCTTTAGCAGACCAAAAAGCAAAACTTAAAGCCTCTTCACATTAATTTTTTTTTTCTTTTCAAAAAGCATATAAAATTACGAATAATAATTGGGTGTTGCCGGTCTCTCACTAAACCCCCCTTTTGCTTTTCTATGATGAAATTCCATTGGTCATCATTAAACTCCATCATTTCTGGCCGTTCGATGTCCCCTTGACAACTCCATCCCGTCCGTCCACTTGTCGGAACGCAAATCATTGTGTTGGGAAGTCATTAGGGGATCCCTCCCCCATTATTGTATTATTTTTTCATGGAATTGCATGCATTTTCTTTTTCTTTTTTTGAAGCTTCTCTTTTATAAATAAATCTATTCGTCGAAAATTGACACTGAAATTATGTTGGTCTCTTCCAATATTTTTTCATTTTGATTTGCATAAAATTTTTCATTACTTTTAATAACTTCCCTTTTATTTATTTTACTTTCATTTTGATATTATTCTTCCATTTTATTTGAATCCTTTTTTTTTTTTGGTTTTTGGGTTCCATGTGTTTTTTTTTGAGTAATTTATAATCATAATATTTAATTTTTCCATCAATATTTATGTATGTCTATAGGGTTGGATATATTACATATGAAAAATAATTAACTTTTTGTTATATCTCATACAAAATTTGACAGAATAGAATACAAATTATCACTAGTGTGGACATATTATTACACACAAGAAAATTTTAAATTACTTTATAAAATGGTAAAATTATATCTATTTTTTCATGATTTTGTTTATTGCTATTATAATTTTTTTCAAAAAAATATTGATCAATACATCGACATTTAAACCGTTTATCGATTTGAAAGTGAATGAGATGAGTGTATTTGTTAAATGAGTATGTGTTAATTTTAATGGTCAGGGATTGATAAGCAATGAGAAACGTGATAGTTTCAACATTCCAGTGTATGCACCAAGTTTGCAAGACTTCAAAGAGGTAGTAGAAGCCGATGGATCATTTTCCATAAACAAACTAGAAGTTTTCAAAGGAGGCAGTCCATTGGTAGTGAACCAACCAGACGATGCAGCAGAGGTCGGTCGAGCTTTGGCCAACAGCTGTCGAAGTGTTTCTGGTGTCCTCGTCGACGCCCATATCGGCGATCGGCTAAGCGAAGAGCTCTTCTATCGAGTCGAACGTAGAGCCACAAACCATGCTAAAGACCTTCTTGAGAAGCTTCAATTCTTTCACATTGTGGCATCACTATCCCTTGCATAAACAAGAGACCAAAATGAAAGAAAAACAAAAAACACAAAAACAAAAAAACAAAAACACTGCCTTCATCTCTCTTTTGGTGGTTGTTCTGTCTTTTAAGGATATGATCATATGTTACATAGAAAACAAAAGGAATCAAACAGGCAAAAGAGAGGTAAAAGGAGCTGCCATTTTCTGTCTTTTTTTTTTTTGTTCATTATAACTTTGTAGTGTGATGTTTTGGGTCATTTTACTGACTATGTAGTTATAAAAATATGTGTTTAGAGTTGATGGGCTTTATTTTTGTTCCCCCTTGGCTTTTGGGAATCCCAAAAAAGAAAATATAGAGCCAAGTCTGTAACCCTCTCTTAGTTTTACTTTCAGTTTCCTTGTAGCTTTTTTTTTTAATGTCTATAAATGTCAAAAGTCTCTTTGTAATGC

mRNA sequence

CTACACAAAAGCTTTCTCATAAAAGACTCTCCCCTTCTCCAAGCTTAAAGATAAAAGCTTCAAACCCTTCTCATGGCTCCCAAGGGCGACAACGTCGTCGTTTCGAACATGAAACTCGAACGAATGCTCAGCATGAAAGGAGGTAAAGGCGAAACGAGCTATGCTAACAACTCTCAAGCTCAGGCTCAACATGCGAGATCAATGCTTCATCTTCTAAAAGAAACATTGGACGGAGTTCATTTAAACTCGCCGGAACTGCCGTTCGTGGTGGTGGATCTCGGCTGCAGCTGCGGCAGCAACACCATTTACATCGTCGACGTCATCATAAAGCACATTATAAAGCGCTTTGAGGCTTTGGCCGTCGACCCGCCGGAGTTCACCGCCTTTTTCTCCGATCTCCCTGGAAATGACTTCAATACACTCTTCCAGCTCCTCCCGCCTTTGGCCACTTATGGCGGCAGCATGGAGGAATGCCTCGCCGCCGACAACCATCGCTCCTACTTCGCTGCCGGCGTTCCTGGCTCCTTCTACCGCCGTCTGTTTCCGGCCAGGTCCATCGACGTACCAGAGACGGTGGTGGATGCGAGATCGACGGCATACAACAGAGGGAGGGTGTTCATCCACGGCGCAAACGAGGCGGCGGCGGCGGCTTACCGGAAGCAATTTCAGACGGACTTGGCCGGATTTCTGTGGGCCAGGGCCCACGAGCTGAAAAGAGGTGGGTCCATGTTCCTTGTCTGCTTGGGCCGGACCTCACTGGACCCCACCGACCAAGGTGGAGCCGGCCTCCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCTTGTCCAAGAGGGATTGATAAGCAATGAGAAACGTGATAGTTTCAACATTCCAGTGTATGCACCAAGTTTGCAAGACTTCAAAGAGGTAGTAGAAGCCGATGGATCATTTTCCATAAACAAACTAGAAGTTTTCAAAGGAGGCAGTCCATTGGTAGTGAACCAACCAGACGATGCAGCAGAGGTCGGTCGAGCTTTGGCCAACAGCTGTCGAAGTGTTTCTGGTGTCCTCGTCGACGCCCATATCGGCGATCGGCTAAGCGAAGAGCTCTTCTATCGAGTCGAACGTAGAGCCACAAACCATGCTAAAGACCTTCTTGAGAAGCTTCAATTCTTTCACATTGTGGCATCACTATCCCTTGCATAAACAAGAGACCAAAATGAAAGAAAAACAAAAAACACAAAAACAAAAAAACAAAAACACTGCCTTCATCTCTCTTTTGGTGGTTGTTCTGTCTTTTAAGGATATGATCATATGTTACATAGAAAACAAAAGGAATCAAACAGGCAAAAGAGAGGTAAAAGGAGCTGCCATTTTCTGTCTTTTTTTTTTTTGTTCATTATAACTTTGTAGTGTGATGTTTTGGGTCATTTTACTGACTATGTAGTTATAAAAATATGTGTTTAGAGTTGATGGGCTTTATTTTTGTTCCCCCTTGGCTTTTGGGAATCCCAAAAAAGAAAATATAGAGCCAAGTCTGTAACCCTCTCTTAGTTTTACTTTCAGTTTCCTTGTAGCTTTTTTTTTTAATGTCTATAAATGTCAAAAGTCTCTTTGTAATGC

Coding sequence (CDS)

ATGGCTCCCAAGGGCGACAACGTCGTCGTTTCGAACATGAAACTCGAACGAATGCTCAGCATGAAAGGAGGTAAAGGCGAAACGAGCTATGCTAACAACTCTCAAGCTCAGGCTCAACATGCGAGATCAATGCTTCATCTTCTAAAAGAAACATTGGACGGAGTTCATTTAAACTCGCCGGAACTGCCGTTCGTGGTGGTGGATCTCGGCTGCAGCTGCGGCAGCAACACCATTTACATCGTCGACGTCATCATAAAGCACATTATAAAGCGCTTTGAGGCTTTGGCCGTCGACCCGCCGGAGTTCACCGCCTTTTTCTCCGATCTCCCTGGAAATGACTTCAATACACTCTTCCAGCTCCTCCCGCCTTTGGCCACTTATGGCGGCAGCATGGAGGAATGCCTCGCCGCCGACAACCATCGCTCCTACTTCGCTGCCGGCGTTCCTGGCTCCTTCTACCGCCGTCTGTTTCCGGCCAGGTCCATCGACGTACCAGAGACGGTGGTGGATGCGAGATCGACGGCATACAACAGAGGGAGGGTGTTCATCCACGGCGCAAACGAGGCGGCGGCGGCGGCTTACCGGAAGCAATTTCAGACGGACTTGGCCGGATTTCTGTGGGCCAGGGCCCACGAGCTGAAAAGAGGTGGGTCCATGTTCCTTGTCTGCTTGGGCCGGACCTCACTGGACCCCACCGACCAAGGTGGAGCCGGCCTCCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCTTGTCCAAGAGGGATTGATAAGCAATGAGAAACGTGATAGTTTCAACATTCCAGTGTATGCACCAAGTTTGCAAGACTTCAAAGAGGTAGTAGAAGCCGATGGATCATTTTCCATAAACAAACTAGAAGTTTTCAAAGGAGGCAGTCCATTGGTAGTGAACCAACCAGACGATGCAGCAGAGGTCGGTCGAGCTTTGGCCAACAGCTGTCGAAGTGTTTCTGGTGTCCTCGTCGACGCCCATATCGGCGATCGGCTAAGCGAAGAGCTCTTCTATCGAGTCGAACGTAGAGCCACAAACCATGCTAAAGACCTTCTTGAGAAGCTTCAATTCTTTCACATTGTGGCATCACTATCCCTTGCATAA

Protein sequence

MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVPETVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVERRATNHAKDLLEKLQFFHIVASLSLA
Homology
BLAST of Lsi02G018800 vs. ExPASy Swiss-Prot
Match: Q9FLN8 (Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 4.9e-162
Identity = 286/385 (74.29%), Postives = 323/385 (83.90%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M  KGDNV V NMKLER+LSMKGGKG+ SYANNSQAQA HARSMLHLL+ETL+ VHLNS 
Sbjct: 1   MGSKGDNVAVCNMKLERLLSMKGGKGQDSYANNSQAQAMHARSMLHLLEETLENVHLNSS 60

Query: 61  EL--PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLF 120
               PF  VDLGCS G+NT++I+D I+KHI KRF+A  +DPPEFTAFFSDLP NDFNTLF
Sbjct: 61  ASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGIDPPEFTAFFSDLPSNDFNTLF 120

Query: 121 QLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VP 180
           QLLPPL +    MEECLAAD +RSYF AGVPGSFYRRLFPAR+ID             VP
Sbjct: 121 QLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLFPARTIDFFHSAFSLHWLSQVP 180

Query: 181 ETVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLG 240
           E+V D RS AYNRGRVFIHGA E    AY++QFQ DLA FL ARA E+KRGG+MFLVCLG
Sbjct: 181 ESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEFLRARAAEVKRGGAMFLVCLG 240

Query: 241 RTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEAD 300
           RTS+DPTDQGGAGLLFGTHFQDAWDDLV+EGL++ EKRD FNIPVYAPSLQDFKEVV+A+
Sbjct: 241 RTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDGFNIPVYAPSLQDFKEVVDAN 300

Query: 301 GSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRV 360
           GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV+GVLV+AHIG+ LS +LF RV
Sbjct: 301 GSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVAGVLVEAHIGEELSNKLFSRV 360

Query: 361 ERRATNHAKDLLEKLQFFHIVASLS 371
           E RAT+HAKD+L  LQFFHIVASLS
Sbjct: 361 ESRATSHAKDVLVNLQFFHIVASLS 384

BLAST of Lsi02G018800 vs. ExPASy Swiss-Prot
Match: Q0J998 (Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 9.3e-137
Identity = 250/395 (63.29%), Postives = 300/395 (75.95%), Query Frame = 0

Query: 4   KGDNVVVS------NMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDG-VH 63
           KG+NV VS        KL  ML MKGG G+ SY NNSQAQA HAR MLH L+ETLD  + 
Sbjct: 9   KGENVTVSAAAAPRMKKLASMLCMKGGNGDGSYLNNSQAQALHARRMLHFLEETLDAMME 68

Query: 64  LNSPELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNT 123
            +S +  F   DLGCSCGSN+++IVDVI++ + + +E+   D PEF  FFSDLP NDFNT
Sbjct: 69  RSSSDKLFTAADLGCSCGSNSLFIVDVIVRRVSEAYESRGRDAPEFQVFFSDLPSNDFNT 128

Query: 124 LFQLLPP-LATYGGSMEECLAADN-----HRSYFAAGVPGSFYRRLFPARSID------- 183
           LFQLLPP LA   GS+EECLAA        R Y AAGVPG+FY RLFP  SID       
Sbjct: 129 LFQLLPPLLAPVAGSLEECLAAGEGAATATRPYHAAGVPGTFYGRLFPGESIDVFTSTFS 188

Query: 184 ------VPETVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGG 243
                 VPE V D+ S AYN GRVF+H A EA AAAY++QFQ DLA FL +RA E+KRGG
Sbjct: 189 LHWLSQVPEEVGDSASPAYNGGRVFVHRATEAVAAAYKRQFQADLARFLRSRAREMKRGG 248

Query: 244 SMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQD 303
           +MFL CLGR+S DP DQGGAGLLFGTHFQDAWDDLVQEG++  EKRDSFNIPVYAPSLQ+
Sbjct: 249 AMFLACLGRSSGDPADQGGAGLLFGTHFQDAWDDLVQEGVVEGEKRDSFNIPVYAPSLQE 308

Query: 304 FKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL 363
           F++VV ADG+F+I++LE+ +GGSPLVV++PDDAAEVGRA+ANSC++V+GVLVDAHIG+R 
Sbjct: 309 FRDVVRADGAFAIDRLELVRGGSPLVVDRPDDAAEVGRAMANSCKAVAGVLVDAHIGERR 368

Query: 364 SEELFYRVERRATNHAKDLLEKLQFFHIVASLSLA 373
             +LF R+ERRA  HA++L+EK+ FFH+V SLSLA
Sbjct: 369 GAQLFERLERRAARHARELVEKMHFFHVVCSLSLA 403

BLAST of Lsi02G018800 vs. ExPASy Swiss-Prot
Match: Q5XF78 (Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 9.8e-54
Identity = 133/379 (35.09%), Postives = 191/379 (50.40%), Query Frame = 0

Query: 15  LERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSPELPFVVVDLGCSCG 74
           L R+ +M+GG+ + SY NNS +QA        +L  +L  + L S + P  + DLGC+ G
Sbjct: 18  LHRVFAMQGGEDDLSYVNNSDSQALAITLSKPILISSLQSIKLFSDQTPIKITDLGCATG 77

Query: 75  SNTIYIVDVIIKHIIKRFEAL--AVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSME 134
           SNT   VD +++ + +R+ A       PEF AFF DLP NDFN LF+LL           
Sbjct: 78  SNTFTTVDTVVETLQRRYTARCGGGGSPEFEAFFCDLPSNDFNMLFKLL----------A 137

Query: 135 ECLAADNHRSYFAAGVPGSFYRRLFPARSI-------------DVPETVVDARSTAYNRG 194
           E    D+   YFA GV GSFY RLFP  +I              +PE V++  S  +N+G
Sbjct: 138 EKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWLSQIPEKVLEKESRTWNKG 197

Query: 195 RVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTDQGG--- 254
           + +I GA +    AY +Q   DL  F+  R  E+ +GG +F++  GR S   +  G    
Sbjct: 198 KTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFVLMAGRPSGSSSQFGDQDT 257

Query: 255 -AGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV 314
            A   F T  + AW DL++EGLI  E RD FNIP Y  S ++    ++  G F I K++ 
Sbjct: 258 RAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEEVTAGIDRCGGFKIGKMDF 317

Query: 315 FKGGSPLVVNQPD--------DAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 367
            K     +V   D        D    GRA  N  ++    +VDA++G  LS ELF R E 
Sbjct: 318 LK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMVDAYLGPDLSHELFKRYEN 377

BLAST of Lsi02G018800 vs. ExPASy Swiss-Prot
Match: A0A061DFA8 (Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 PE=3 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-50
Identity = 130/355 (36.62%), Postives = 185/355 (52.11%), Query Frame = 0

Query: 15  LERMLSMKGGKGETSYANNSQAQAQ-HARSMLHLLKETLDGVHLNSPELPFVVVDLGCSC 74
           +E++LSM GG GE SYANNS  Q+   +RSM  L +  LD    N PE    + DLGCS 
Sbjct: 1   MEQILSMNGGSGEQSYANNSIGQSTCLSRSMPVLKQAVLDSCCTNLPE-TVTMADLGCSS 60

Query: 75  GSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEE 134
           G NT Y V  I   I KR   L    PEF  F +DLPGNDFNT+FQ LP    Y G + E
Sbjct: 61  GPNTFYAVSEITSIIYKRCCQLGRSAPEFRVFLNDLPGNDFNTVFQSLP---AYQGKLRE 120

Query: 135 CLAADNHRSY---FAAGVPGSFYRRLFPARSI-------------DVPETVVDARSTAYN 194
               +N   +   + AGVPGSFY RLFP +++              VP  + D  +   N
Sbjct: 121 ----ENGPGFGPLYVAGVPGSFYGRLFPLKTLHFVHSSSSLHWLSQVPPELTDKANPLIN 180

Query: 195 RGRVFIHGAN-EAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTDQGG 254
           + ++FI   +  A  ++Y  QF+ D + FL  R+ E+   G M L   GRT+ DPT    
Sbjct: 181 KWKIFISKTSPPAVISSYLTQFRKDFSLFLKLRSEEVAPEGRMVLTFRGRTTADPTSD-- 240

Query: 255 AGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEVF 314
              L   +   A+ DLV EGL+  E+ D++N P Y P  ++ +  +E +GSF++++LEV 
Sbjct: 241 ESCLLWDYLGQAFQDLVAEGLVEEERLDTYNTPYYEPHPEEIQAEIEKEGSFTLDRLEVI 300

Query: 315 -------KGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYR 345
                   GG        DD A   + +A + R+V+  +  +H G+ + + LF+R
Sbjct: 301 ALPWDSVNGGI------KDDRATTAKRMAKAIRAVNESMFRSHFGEDILDPLFHR 339

BLAST of Lsi02G018800 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 8.9e-47
Identity = 126/375 (33.60%), Postives = 196/375 (52.27%), Query Frame = 0

Query: 13  MKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSPELPFVVVDLGCS 72
           M++  +L M GG G++SYANNS  Q +       + ++ +  ++ +       + DLGCS
Sbjct: 1   MEVVEVLHMNGGNGDSSYANNSLVQQKVILMTKPITEQAMIDLYSSLFPETLCIADLGCS 60

Query: 73  CGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSME 132
            G+NT  +V  I+K + K  +      PEF   F+DLPGNDFNTLFQ L       G+ +
Sbjct: 61  LGANTFLVVSQIVKIVEKERKKHGFKSPEFYFHFNDLPGNDFNTLFQSL-------GAFQ 120

Query: 133 ECLAADNHRSY---FAAGVPGSFYRRLFPARSI-------------DVPETVVDARSTAY 192
           E L      S+   F +GVPGSFY RLFP++S+              VP  + +      
Sbjct: 121 EDLRKHIGESFGPCFFSGVPGSFYTRLFPSKSLHFVYSSYSLMWLSQVPNGIEN------ 180

Query: 193 NRGRVFIHGANE-AAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTDQG 252
           N+G +++   +  +   AY KQ++ D + FL  R+ EL +GG M L  LGR S DPT + 
Sbjct: 181 NKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEELMKGGKMVLTLLGRESEDPTSK- 240

Query: 253 GAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV 312
                       A ++LV+EGLI  EK D+FNIP Y PS  + K +VE +GSF+IN+LE 
Sbjct: 241 -ECCYIWELLAMALNELVKEGLIKEEKVDAFNIPQYTPSPAEVKYLVEKEGSFTINRLET 300

Query: 313 FKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVERRATNHAKD 371
               S +  N  ++    G  ++   R+V+  L+ +H    L + +F++ E   ++    
Sbjct: 301 ----SRVHWNASNNVKNGGYNVSRCMRAVAEPLLVSHFDKELMDLVFHKYEEIISDCMSK 354

BLAST of Lsi02G018800 vs. ExPASy TrEMBL
Match: A0A0A0K2W3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 1.7e-205
Identity = 365/385 (94.81%), Postives = 366/385 (95.06%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E PFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EEPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDLFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLAGFLWARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAGFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. ExPASy TrEMBL
Match: A0A5D3DHL8 (Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00180 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.7e-205
Identity = 365/385 (94.81%), Postives = 366/385 (95.06%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLA FLWARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAEFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. ExPASy TrEMBL
Match: A0A1S3BIY7 (indole-3-acetate O-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103490122 PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.1e-204
Identity = 364/385 (94.55%), Postives = 365/385 (94.81%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFT FFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTVFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLA FLWARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAEFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. ExPASy TrEMBL
Match: A0A6J1F711 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442937 PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 5.4e-204
Identity = 362/385 (94.03%), Postives = 368/385 (95.58%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYANNSQAQAQHARS+LHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           ELPFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRV IHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLIS+EKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. ExPASy TrEMBL
Match: A0A6J1IFI9 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476788 PE=4 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.5e-202
Identity = 358/385 (92.99%), Postives = 367/385 (95.32%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           MAPKGDNV+VSN+KLERMLSMKGGKGE SYANNSQAQAQHARS+LHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVIVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           ELPFVVVDLGCSCGSNTIYIVDVIIKHI+KRFEALA+DPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIVKRFEALALDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAA+NHRSYFAAGVPGSFYRRLFPARSID             VPE+
Sbjct: 121 LPPLATYGGSMEECLAANNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRV IHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLIS+EKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALA SCRSVSGVLVDAHIGDRLSEELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALAISCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. NCBI nr
Match: XP_038897278.1 (indole-3-acetate O-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 731.1 bits (1886), Expect = 4.8e-207
Identity = 367/385 (95.32%), Postives = 370/385 (96.10%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDTRSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           F INKLEVFKGGSPLVVNQPD+AAEVGRALANSCRS+SGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FYINKLEVFKGGSPLVVNQPDNAAEVGRALANSCRSISGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. NCBI nr
Match: XP_004151408.1 (indole-3-acetate O-methyltransferase 1 [Cucumis sativus] >KGN43990.1 hypothetical protein Csa_016269 [Cucumis sativus])

HSP 1 Score: 724.9 bits (1870), Expect = 3.4e-205
Identity = 365/385 (94.81%), Postives = 366/385 (95.06%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E PFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EEPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDLFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLAGFLWARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAGFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. NCBI nr
Match: KAA0048120.1 (indole-3-acetate O-methyltransferase 1 [Cucumis melo var. makuwa] >TYK23052.1 indole-3-acetate O-methyltransferase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 723.8 bits (1867), Expect = 7.7e-205
Identity = 365/385 (94.81%), Postives = 366/385 (95.06%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLA FLWARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAEFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. NCBI nr
Match: XP_008447710.1 (PREDICTED: indole-3-acetate O-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 722.2 bits (1863), Expect = 2.2e-204
Identity = 364/385 (94.55%), Postives = 365/385 (94.81%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFT FFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTVFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPET
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLA FLWARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAEFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. NCBI nr
Match: XP_022936266.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 719.9 bits (1857), Expect = 1.1e-203
Identity = 362/385 (94.03%), Postives = 368/385 (95.58%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYANNSQAQAQHARS+LHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           ELPFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VPET 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID             VPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRV IHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLIS+EKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 373
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lsi02G018800 vs. TAIR 10
Match: AT5G55250.1 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 572.0 bits (1473), Expect = 3.5e-163
Identity = 286/385 (74.29%), Postives = 323/385 (83.90%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60
           M  KGDNV V NMKLER+LSMKGGKG+ SYANNSQAQA HARSMLHLL+ETL+ VHLNS 
Sbjct: 1   MGSKGDNVAVCNMKLERLLSMKGGKGQDSYANNSQAQAMHARSMLHLLEETLENVHLNSS 60

Query: 61  EL--PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLF 120
               PF  VDLGCS G+NT++I+D I+KHI KRF+A  +DPPEFTAFFSDLP NDFNTLF
Sbjct: 61  ASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGIDPPEFTAFFSDLPSNDFNTLF 120

Query: 121 QLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID-------------VP 180
           QLLPPL +    MEECLAAD +RSYF AGVPGSFYRRLFPAR+ID             VP
Sbjct: 121 QLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLFPARTIDFFHSAFSLHWLSQVP 180

Query: 181 ETVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLG 240
           E+V D RS AYNRGRVFIHGA E    AY++QFQ DLA FL ARA E+KRGG+MFLVCLG
Sbjct: 181 ESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEFLRARAAEVKRGGAMFLVCLG 240

Query: 241 RTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEAD 300
           RTS+DPTDQGGAGLLFGTHFQDAWDDLV+EGL++ EKRD FNIPVYAPSLQDFKEVV+A+
Sbjct: 241 RTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDGFNIPVYAPSLQDFKEVVDAN 300

Query: 301 GSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRV 360
           GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV+GVLV+AHIG+ LS +LF RV
Sbjct: 301 GSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVAGVLVEAHIGEELSNKLFSRV 360

Query: 361 ERRATNHAKDLLEKLQFFHIVASLS 371
           E RAT+HAKD+L  LQFFHIVASLS
Sbjct: 361 ESRATSHAKDVLVNLQFFHIVASLS 384

BLAST of Lsi02G018800 vs. TAIR 10
Match: AT5G55250.2 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 513.5 bits (1321), Expect = 1.5e-145
Identity = 255/346 (73.70%), Postives = 290/346 (83.82%), Query Frame = 0

Query: 40  HARSMLHLLKETLDGVHLNSPEL--PFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAV 99
           HARSMLHLL+ETL+ VHLNS     PF  VDLGCS G+NT++I+D I+KHI KRF+A  +
Sbjct: 2   HARSMLHLLEETLENVHLNSSASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGI 61

Query: 100 DPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 159
           DPPEFTAFFSDLP NDFNTLFQLLPPL +    MEECLAAD +RSYF AGVPGSFYRRLF
Sbjct: 62  DPPEFTAFFSDLPSNDFNTLFQLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLF 121

Query: 160 PARSID-------------VPETVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAG 219
           PAR+ID             VPE+V D RS AYNRGRVFIHGA E    AY++QFQ DLA 
Sbjct: 122 PARTIDFFHSAFSLHWLSQVPESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAE 181

Query: 220 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRD 279
           FL ARA E+KRGG+MFLVCLGRTS+DPTDQGGAGLLFGTHFQDAWDDLV+EGL++ EKRD
Sbjct: 182 FLRARAAEVKRGGAMFLVCLGRTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRD 241

Query: 280 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 339
            FNIPVYAPSLQDFKEVV+A+GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV
Sbjct: 242 GFNIPVYAPSLQDFKEVVDANGSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSV 301

Query: 340 SGVLVDAHIGDRLSEELFYRVERRATNHAKDLLEKLQFFHIVASLS 371
           +GVLV+AHIG+ LS +LF RVE RAT+HAKD+L  LQFFHIVASLS
Sbjct: 302 AGVLVEAHIGEELSNKLFSRVESRATSHAKDVLVNLQFFHIVASLS 346

BLAST of Lsi02G018800 vs. TAIR 10
Match: AT5G56300.1 (gibberellic acid methyltransferase 2 )

HSP 1 Score: 212.2 bits (539), Expect = 7.0e-55
Identity = 133/379 (35.09%), Postives = 191/379 (50.40%), Query Frame = 0

Query: 15  LERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSPELPFVVVDLGCSCG 74
           L R+ +M+GG+ + SY NNS +QA        +L  +L  + L S + P  + DLGC+ G
Sbjct: 18  LHRVFAMQGGEDDLSYVNNSDSQALAITLSKPILISSLQSIKLFSDQTPIKITDLGCATG 77

Query: 75  SNTIYIVDVIIKHIIKRFEAL--AVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSME 134
           SNT   VD +++ + +R+ A       PEF AFF DLP NDFN LF+LL           
Sbjct: 78  SNTFTTVDTVVETLQRRYTARCGGGGSPEFEAFFCDLPSNDFNMLFKLL----------A 137

Query: 135 ECLAADNHRSYFAAGVPGSFYRRLFPARSI-------------DVPETVVDARSTAYNRG 194
           E    D+   YFA GV GSFY RLFP  +I              +PE V++  S  +N+G
Sbjct: 138 EKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWLSQIPEKVLEKESRTWNKG 197

Query: 195 RVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTDQGG--- 254
           + +I GA +    AY +Q   DL  F+  R  E+ +GG +F++  GR S   +  G    
Sbjct: 198 KTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFVLMAGRPSGSSSQFGDQDT 257

Query: 255 -AGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV 314
            A   F T  + AW DL++EGLI  E RD FNIP Y  S ++    ++  G F I K++ 
Sbjct: 258 RAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEEVTAGIDRCGGFKIGKMDF 317

Query: 315 FKGGSPLVVNQPD--------DAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 367
            K     +V   D        D    GRA  N  ++    +VDA++G  LS ELF R E 
Sbjct: 318 LK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMVDAYLGPDLSHELFKRYEN 377

BLAST of Lsi02G018800 vs. TAIR 10
Match: AT4G26420.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-46
Identity = 129/384 (33.59%), Postives = 186/384 (48.44%), Query Frame = 0

Query: 11  SNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP-ELPFVVVDL 70
           S+  LE +LSM+GG+ + SY  N    A        +L   ++ + L         + DL
Sbjct: 3   SSRSLEHVLSMQGGEDDASYVKNCYGPAARLALSKPMLTTAINSIKLTEGCSSHLKIADL 62

Query: 71  GCSCGSNTIYIVDVIIKHIIKRFEAL--AVDPP-EFTAFFSDLPGNDFNTLFQLLPPLAT 130
           GC+ G NT   V+ +++ + K+   +    +P  EF  FFSDL  NDFN LF+       
Sbjct: 63  GCAIGDNTFSTVETVVEVLGKKLAVIDGGTEPEMEFEVFFSDLSSNDFNALFR------- 122

Query: 131 YGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSI-------------DVPETVVDARS 190
              S++E +   + R YFAAGVPGSFY+RLFP   +              VPE V++  S
Sbjct: 123 ---SLDEKVNGSS-RKYFAAGVPGSFYKRLFPKGELHVVVTMSALQWLSQVPEKVMEKGS 182

Query: 191 TAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTD 250
            ++N+G V+I GA +    AY +Q   DL  FL  R  E+  GG +F++  GR S     
Sbjct: 183 KSWNKGGVWIEGAEKEVVEAYAEQADKDLVEFLKCRKEEIVVGGVLFMLMGGRPSGSVNQ 242

Query: 251 QGGAGLL----FGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFS 310
            G         F T    AW DLV EGLI  EKRD FNIPVY  + ++    ++  G F 
Sbjct: 243 IGDPDSSLKHPFTTLMDQAWQDLVDEGLIEEEKRDGFNIPVYFRTTEEIAAAIDRCGGFK 302

Query: 311 INKLE------VFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFY 368
           I K E         G    ++  PD     GR  AN  ++    +V A++G  L+ +LF 
Sbjct: 303 IEKTENLIIADHMNGKQEELMKDPD---SYGRDRANYAQAGLKPIVQAYLGPDLTHKLFK 362

BLAST of Lsi02G018800 vs. TAIR 10
Match: AT5G38020.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 4.2e-44
Identity = 125/375 (33.33%), Postives = 181/375 (48.27%), Query Frame = 0

Query: 18  MLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSPELP--FVVVDLGCSCGS 77
           +LSMKGG GE SYANNS+ Q + A     ++ ET+  + + + + P    V DLGCS G 
Sbjct: 3   VLSMKGGDGEHSYANNSEGQKRLASDAKPVVVETVKEMIVKT-DFPGCIKVADLGCSSGE 62

Query: 78  NTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECL 137
           NT+ ++  I+  II  ++    + PE     +DLP NDFNT F+L+P       +  + L
Sbjct: 63  NTLLVMSEIVNTIITSYQQKGKNLPEINCCLNDLPDNDFNTTFKLVP-------AFHKLL 122

Query: 138 AADNHRSYFAAGVPGSFYRRLFPARSI-------------DVPETVVDARSTAYNRGRVF 197
             D     F +GVPGSFY RLFP++S+              VP+ + D +   Y R    
Sbjct: 123 KMDVKGKCFISGVPGSFYSRLFPSKSLHFVHSSLCLHWLSKVPDGLEDNKKNVYLRS--- 182

Query: 198 IHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFG 257
                     +Y  QF+ D + FL  RA E    G M L  +GR SLDP  +        
Sbjct: 183 --PCPPNVYKSYLTQFKNDFSLFLRLRADETVPNGRMALTFVGRKSLDPLSKD--CFQNW 242

Query: 258 THFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV------- 317
           +   D+  DLV EG++     DSFN+P Y P   + +EV+E++GSF I+  E        
Sbjct: 243 SSISDSLLDLVSEGIVKESDVDSFNLPFYNPDESEVREVIESEGSFKISNFETIFGLLFS 302

Query: 318 FKGGSPLVVNQPDD-----AAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVERRAT 364
           +K G   V +  D+       EV R  A+  RS++  ++ AH GD + + LF R      
Sbjct: 303 YKTGRTEVKDDDDNLDQSCRFEVIRKRASIIRSITEPMLGAHFGDAIMDRLFERYTYHLA 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLN84.9e-16274.29Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 ... [more]
Q0J9989.3e-13763.29Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5XF789.8e-5435.09Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE... [more]
A0A061DFA83.0e-5036.62Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 P... [more]
A4ZDG88.9e-4733.60S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
Match NameE-valueIdentityDescription
A0A0A0K2W31.7e-20594.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1[more]
A0A5D3DHL83.7e-20594.81Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BIY71.1e-20494.55indole-3-acetate O-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103490122 P... [more]
A0A6J1F7115.4e-20494.03indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IFI96.5e-20292.99indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_038897278.14.8e-20795.32indole-3-acetate O-methyltransferase 1 [Benincasa hispida][more]
XP_004151408.13.4e-20594.81indole-3-acetate O-methyltransferase 1 [Cucumis sativus] >KGN43990.1 hypothetica... [more]
KAA0048120.17.7e-20594.81indole-3-acetate O-methyltransferase 1 [Cucumis melo var. makuwa] >TYK23052.1 in... [more]
XP_008447710.12.2e-20494.55PREDICTED: indole-3-acetate O-methyltransferase 1 [Cucumis melo][more]
XP_022936266.11.1e-20394.03indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G55250.13.5e-16374.29IAA carboxylmethyltransferase 1 [more]
AT5G55250.21.5e-14573.70IAA carboxylmethyltransferase 1 [more]
AT5G56300.17.0e-5535.09gibberellic acid methyltransferase 2 [more]
AT4G26420.11.2e-4633.59S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G38020.14.2e-4433.33S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 33..299
e-value: 2.1E-106
score: 357.6
NoneNo IPR availablePANTHERPTHR31009:SF42INDOLE-3-ACETATE O-METHYLTRANSFERASE 1coord: 10..371
IPR042086Methyltransferase, alpha-helical capping domainGENE3D1.10.1200.270coord: 18..354
e-value: 2.1E-106
score: 357.6
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 62..370
e-value: 1.2E-87
score: 294.3
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 10..371
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 13..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G018800.1Lsi02G018800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity