Lsi02G001120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G001120
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	transmembrane protein adipocyte-associated 1 homolog
Location	chr02: 908025 .. 908592 (+)
RNA-Seq Expression	Lsi02G001120
Synteny	Lsi02G001120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTGCATGATCTGAGTTCAGTAGATCGCAATTTCGCTGCTCCCAGAAATGGGGACGATTCTCCAAGCAGTTTACAGTGCCTCAGACCCTTTGTCGCCTATTGGGAGTTTTTCATCTCAGCCCAATGGGGACAACCGGGGACTCACTTCCGTCACTTCCGCCTCCGGTTGCCATGGAATGTGGTATAGTGCTCTCTTGGTTGCGCCCTCGGTTTTGTTCGCCATCTATTTGGCGATTAGTGCCATCAAGAACATGAAGAAGTATTTTCTTGGTCGGTCGTTTATAATGATGTCGTATTATGCCCTTCTCTGGATCACTACGCTCCTAAATCTTGCTTGGTGCTCTCTTCAGGTGAGGTGGACTTTAACGAAGTATAGTTTGCTTTGATTTAATGAGCTCTCTTGCACGACGGTAATATAGGATTTGTGTGTCGGGTACCTGCACATAATAATTCGTTTTTCATTTTGAGTTTATTCCTAAACAGAGCCTAGTTGCATCCAACCACAACAACTTGGTCTTCAAATCCTTGTTGATCCCATGGAAACCTGAAGACAAAGCCATCTGA

mRNA sequence

ATGGAAATGGGGACGATTCTCCAAGCAGTTTACAGTGCCTCAGACCCTTTGTCGCCTATTGGGAGTTTTTCATCTCAGCCCAATGGGGACAACCGGGGACTCACTTCCGTCACTTCCGCCTCCGGTTGCCATGGAATGTGGTATAGTGCTCTCTTGGTTGCGCCCTCGGTTTTGTTCGCCATCTATTTGGCGATTAGTGCCATCAAGAACATGAAGAAGTATTTTCTTGGTCGGTCGTTTATAATGATGTCGTATTATGCCCTTCTCTGGATCACTACGCTCCTAAATCTTGCTTGGTGCTCTCTTCAGAGCCTAGTTGCATCCAACCACAACAACTTGGTCTTCAAATCCTTGTTGATCCCATGGAAACCTGAAGACAAAGCCATCTGA

Coding sequence (CDS)

ATGGAAATGGGGACGATTCTCCAAGCAGTTTACAGTGCCTCAGACCCTTTGTCGCCTATTGGGAGTTTTTCATCTCAGCCCAATGGGGACAACCGGGGACTCACTTCCGTCACTTCCGCCTCCGGTTGCCATGGAATGTGGTATAGTGCTCTCTTGGTTGCGCCCTCGGTTTTGTTCGCCATCTATTTGGCGATTAGTGCCATCAAGAACATGAAGAAGTATTTTCTTGGTCGGTCGTTTATAATGATGTCGTATTATGCCCTTCTCTGGATCACTACGCTCCTAAATCTTGCTTGGTGCTCTCTTCAGAGCCTAGTTGCATCCAACCACAACAACTTGGTCTTCAAATCCTTGTTGATCCCATGGAAACCTGAAGACAAAGCCATCTGA

Protein sequence

MEMGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIYLAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQSLVASNHNNLVFKSLLIPWKPEDKAI

Homology

[Close]

BLAST of Lsi02G001120 vs. ExPASy Swiss-Prot
Match: Q94AH1 (Protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 2 OS=Arabidopsis thaliana OX=3702 GN=CAND2 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.9e-10
Identity = 32/78 (41.03%), Postives = 49/78 (62.82%), Query Frame = 0


Query: 43  CHGMWYSALLVAPSVLFAIYLAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSL 102
           CHG  ++ +LV  S+LF  YLA  A K++ K    RS+IM++YY  LW+ +LLNLAWC L
Sbjct: 35  CHGFLHNTVLVLASILFVAYLAYEAKKSLSKLSNRRSYIMIAYYGFLWLVSLLNLAWCCL 94

Query: 103 QSLVASNHNNLVFKSLLI 121
           Q+   +    +++  L +
Sbjct: 95  QAWECTPGKEVIWNLLTL 112

[Close]

BLAST of Lsi02G001120 vs. ExPASy Swiss-Prot
Match: F4K2U8 (Protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 8 OS=Arabidopsis thaliana OX=3702 GN=CAND8 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.7e-10
Identity = 33/78 (42.31%), Postives = 48/78 (61.54%), Query Frame = 0


Query: 43  CHGMWYSALLVAPSVLFAIYLAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSL 102
           CHG  ++ +LVA S+ F  YLA  A K++ K    RS+IM++YY  LW+ +LLNLAWC L
Sbjct: 32  CHGFLHNTVLVAASLFFVAYLAYEAKKSLSKLSNRRSYIMIAYYGCLWLVSLLNLAWCCL 91

Query: 103 QSLVASNHNNLVFKSLLI 121
           Q    +    +V+  L +
Sbjct: 92  QGWECTPGKEVVWNLLTL 109

[Close]

BLAST of Lsi02G001120 vs. ExPASy TrEMBL
Match: A0A0A0LSU2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G039110 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-36
Identity = 85/101 (84.16%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTIL  V+S+SD LSPIGSFSSQ NGD RGLT+  ++SGCHGMWYSALLVAPSVLFAIY
Sbjct: 1   MGTILLPVFSSSDALSPIGSFSSQLNGDVRGLTA--ASSGCHGMWYSALLVAPSVLFAIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LAISAI+N+KK+FLGRSFIM+SYYALLWITTLLNLAWCSLQ
Sbjct: 61  LAISAIRNIKKFFLGRSFIMISYYALLWITTLLNLAWCSLQ 99

[Close]

BLAST of Lsi02G001120 vs. ExPASy TrEMBL
Match: A0A5D3BL77 (Transmembrane protein adipocyte-associated 1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001280 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 9.7e-36
Identity = 85/101 (84.16%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTIL  VYS+SD LSPIGSFSSQ +GD RGLT+  ++SGCH MWYSALLVAPSVLFAIY
Sbjct: 1   MGTILVPVYSSSDALSPIGSFSSQLSGDVRGLTA--ASSGCHVMWYSALLVAPSVLFAIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LAISAIKN+KKYFLGRSFIM+SYY LLWITTLLNLAWCSLQ
Sbjct: 61  LAISAIKNIKKYFLGRSFIMISYYVLLWITTLLNLAWCSLQ 99

[Close]

BLAST of Lsi02G001120 vs. ExPASy TrEMBL
Match: A0A1S3BKM5 (transmembrane protein adipocyte-associated 1 homolog OS=Cucumis melo OX=3656 GN=LOC103490687 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 9.7e-36
Identity = 85/101 (84.16%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTIL  VYS+SD LSPIGSFSSQ +GD RGLT+  ++SGCH MWYSALLVAPSVLFAIY
Sbjct: 1   MGTILVPVYSSSDALSPIGSFSSQLSGDVRGLTA--ASSGCHVMWYSALLVAPSVLFAIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LAISAIKN+KKYFLGRSFIM+SYY LLWITTLLNLAWCSLQ
Sbjct: 61  LAISAIKNIKKYFLGRSFIMISYYVLLWITTLLNLAWCSLQ 99

[Close]

BLAST of Lsi02G001120 vs. ExPASy TrEMBL
Match: A0A6J1EDV1 (uncharacterized protein LOC111431639 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431639 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.4e-34
Identity = 82/116 (70.69%), Postives = 93/116 (80.17%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTILQAVYS SDPLSPIG+       D+RG T+VTSAS CHGMWYSALLVAPSVLF IY
Sbjct: 1   MGTILQAVYSVSDPLSPIGT------PDDRGFTAVTSASDCHGMWYSALLVAPSVLFVIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQSLVASNHNNLVFKSL 119
           LA SAIKNMK++F+GRSFIM+ YYALLWI TLLNLAWCSLQ+   S    +++  L
Sbjct: 61  LATSAIKNMKRFFVGRSFIMILYYALLWIATLLNLAWCSLQAWECSRGKEVLWNIL 110

[Close]

BLAST of Lsi02G001120 vs. ExPASy TrEMBL
Match: A0A6J1E884 (uncharacterized protein LOC111431639 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431639 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.1e-34
Identity = 80/101 (79.21%), Postives = 87/101 (86.14%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTILQAVYS SDPLSPIG+       D+RG T+VTSAS CHGMWYSALLVAPSVLF IY
Sbjct: 1   MGTILQAVYSVSDPLSPIGT------PDDRGFTAVTSASDCHGMWYSALLVAPSVLFVIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LA SAIKNMK++F+GRSFIM+ YYALLWI TLLNLAWCSLQ
Sbjct: 61  LATSAIKNMKRFFVGRSFIMILYYALLWIATLLNLAWCSLQ 95

[Close]

BLAST of Lsi02G001120 vs. NCBI nr
Match: XP_004137428.1 (protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 2 [Cucumis sativus] >KGN64044.1 hypothetical protein Csa_013150 [Cucumis sativus])

HSP 1 Score: 162.2 bits (409), Expect = 3.1e-36
Identity = 85/101 (84.16%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTIL  V+S+SD LSPIGSFSSQ NGD RGLT+  ++SGCHGMWYSALLVAPSVLFAIY
Sbjct: 1   MGTILLPVFSSSDALSPIGSFSSQLNGDVRGLTA--ASSGCHGMWYSALLVAPSVLFAIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LAISAI+N+KK+FLGRSFIM+SYYALLWITTLLNLAWCSLQ
Sbjct: 61  LAISAIRNIKKFFLGRSFIMISYYALLWITTLLNLAWCSLQ 99

[Close]

BLAST of Lsi02G001120 vs. NCBI nr
Match: XP_008448577.1 (PREDICTED: transmembrane protein adipocyte-associated 1 homolog [Cucumis melo])

HSP 1 Score: 159.5 bits (402), Expect = 2.0e-35
Identity = 85/101 (84.16%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTIL  VYS+SD LSPIGSFSSQ +GD RGLT+  ++SGCH MWYSALLVAPSVLFAIY
Sbjct: 1   MGTILVPVYSSSDALSPIGSFSSQLSGDVRGLTA--ASSGCHVMWYSALLVAPSVLFAIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LAISAIKN+KKYFLGRSFIM+SYY LLWITTLLNLAWCSLQ
Sbjct: 61  LAISAIKNIKKYFLGRSFIMISYYVLLWITTLLNLAWCSLQ 99

[Close]

BLAST of Lsi02G001120 vs. NCBI nr
Match: TYJ98928.1 (transmembrane protein adipocyte-associated 1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 159.5 bits (402), Expect = 2.0e-35
Identity = 85/101 (84.16%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTIL  VYS+SD LSPIGSFSSQ +GD RGLT+  ++SGCH MWYSALLVAPSVLFAIY
Sbjct: 1   MGTILVPVYSSSDALSPIGSFSSQLSGDVRGLTA--ASSGCHVMWYSALLVAPSVLFAIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LAISAIKN+KKYFLGRSFIM+SYY LLWITTLLNLAWCSLQ
Sbjct: 61  LAISAIKNIKKYFLGRSFIMISYYVLLWITTLLNLAWCSLQ 99

[Close]

BLAST of Lsi02G001120 vs. NCBI nr
Match: XP_023519639.1 (uncharacterized protein LOC111783005 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 156.4 bits (394), Expect = 1.7e-34
Identity = 83/116 (71.55%), Postives = 94/116 (81.03%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTILQAVYS SDPLSPIG+       D+RG T+VTSAS CHGMWYSALLVAPSVLF IY
Sbjct: 1   MGTILQAVYSVSDPLSPIGT------PDDRGFTAVTSASDCHGMWYSALLVAPSVLFVIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQSLVASNHNNLVFKSL 119
           LA SAIKNMK++F+GRSFIM+SYYALLWI TLLNLAWCSLQ+   S    +++  L
Sbjct: 61  LATSAIKNMKRFFVGRSFIMISYYALLWIATLLNLAWCSLQAWECSPGKEVLWNIL 110

[Close]

BLAST of Lsi02G001120 vs. NCBI nr
Match: XP_023519638.1 (uncharacterized protein LOC111783005 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 155.2 bits (391), Expect = 3.8e-34
Identity = 81/101 (80.20%), Postives = 88/101 (87.13%), Query Frame = 0


Query: 3   MGTILQAVYSASDPLSPIGSFSSQPNGDNRGLTSVTSASGCHGMWYSALLVAPSVLFAIY 62
           MGTILQAVYS SDPLSPIG+       D+RG T+VTSAS CHGMWYSALLVAPSVLF IY
Sbjct: 1   MGTILQAVYSVSDPLSPIGT------PDDRGFTAVTSASDCHGMWYSALLVAPSVLFVIY 60

Query: 63  LAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSLQ 104
           LA SAIKNMK++F+GRSFIM+SYYALLWI TLLNLAWCSLQ
Sbjct: 61  LATSAIKNMKRFFVGRSFIMISYYALLWIATLLNLAWCSLQ 95

[Close]

BLAST of Lsi02G001120 vs. TAIR 10
Match: AT3G05010.1 (Protein of unknown function, transmembrane-40 )

HSP 1 Score: 65.9 bits (159), Expect = 2.8e-11
Identity = 32/78 (41.03%), Postives = 49/78 (62.82%), Query Frame = 0


Query: 43  CHGMWYSALLVAPSVLFAIYLAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSL 102
           CHG  ++ +LV  S+LF  YLA  A K++ K    RS+IM++YY  LW+ +LLNLAWC L
Sbjct: 35  CHGFLHNTVLVLASILFVAYLAYEAKKSLSKLSNRRSYIMIAYYGFLWLVSLLNLAWCCL 94

Query: 103 QSLVASNHNNLVFKSLLI 121
           Q+   +    +++  L +
Sbjct: 95  QAWECTPGKEVIWNLLTL 112

[Close]

BLAST of Lsi02G001120 vs. TAIR 10
Match: AT5G27210.1 (Protein of unknown function, transmembrane-40 )

HSP 1 Score: 65.1 bits (157), Expect = 4.8e-11
Identity = 33/78 (42.31%), Postives = 48/78 (61.54%), Query Frame = 0


Query: 43  CHGMWYSALLVAPSVLFAIYLAISAIKNMKKYFLGRSFIMMSYYALLWITTLLNLAWCSL 102
           CHG  ++ +LVA S+ F  YLA  A K++ K    RS+IM++YY  LW+ +LLNLAWC L
Sbjct: 32  CHGFLHNTVLVAASLFFVAYLAYEAKKSLSKLSNRRSYIMIAYYGCLWLVSLLNLAWCCL 91

Query: 103 QSLVASNHNNLVFKSLLI 121
           Q    +    +V+  L +
Sbjct: 92  QGWECTPGKEVVWNLLTL 109

The following BLAST results are available for this feature:

BLAST of Lsi02G001120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AH1	3.9e-10	41.03	Protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 2 OS=Arabidopsis thaliana OX=3702 G...	[more]
F4K2U8	6.7e-10	42.31	Protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 8 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Lsi02G001120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSU2	1.5e-36	84.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G039110 PE=3 SV=1	[more]
A0A5D3BL77	9.7e-36	84.16	Transmembrane protein adipocyte-associated 1-like protein OS=Cucumis melo var. m...	[more]
A0A1S3BKM5	9.7e-36	84.16	transmembrane protein adipocyte-associated 1 homolog OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1EDV1	2.4e-34	70.69	uncharacterized protein LOC111431639 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1E884	9.1e-34	79.21	uncharacterized protein LOC111431639 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Lsi02G001120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137428.1	3.1e-36	84.16	protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 2 [Cucumis sativus] >KGN64044.1 hyp...	[more]
XP_008448577.1	2.0e-35	84.16	PREDICTED: transmembrane protein adipocyte-associated 1 homolog [Cucumis melo]	[more]
TYJ98928.1	2.0e-35	84.16	transmembrane protein adipocyte-associated 1-like protein [Cucumis melo var. mak...	[more]
XP_023519639.1	1.7e-34	71.55	uncharacterized protein LOC111783005 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023519638.1	3.8e-34	80.20	uncharacterized protein LOC111783005 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G001120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05010.1	2.8e-11	41.03	Protein of unknown function, transmembrane-40	[more]
AT5G27210.1	4.8e-11	42.31	Protein of unknown function, transmembrane-40	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018781	Transmembrane protein TPRA1/CAND2/CAND8	PFAM	PF10160	Tmemb_40	coord: 42..118 e-value: 3.7E-19 score: 69.2
IPR018781	Transmembrane protein TPRA1/CAND2/CAND8	PANTHER	PTHR15876	TRANSMEMBRANE PROTEIN ADIPOCYTE-ASSOCIATED 1	coord: 11..115
None	No IPR available	PANTHER	PTHR15876:SF10	TRANSMEMBRANE PROTEIN ADIPOCYTE-ASSOCIATED 1-RELATED	coord: 11..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G001120.1	Lsi02G001120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007186	G protein-coupled receptor signaling pathway
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004930	G protein-coupled receptor activity