Lsi01G020220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G020220
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionTranslation factor GUF1 homolog, chloroplastic
Locationchr01: 27645742 .. 27650514 (-)
RNA-Seq ExpressionLsi01G020220
SyntenyLsi01G020220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTTTAGGTACAGAGAAAGTGAATTGATAAAACTGGACATTCAGATAAATGGTGATCGAGTGGAACCATTGGCAACAATAGTGCACAGCAATAAGGTAATACTTATATAAAAAATACATGCTGTCATAACTTTTGTTTTACAATCTCCCATGCTATCATTCGAGAAATAAATTTTGCTTATGTTAGGTTTCCGAACTCCTACAAACTGAAACCCAACGTTTCCAAAGAACAGGGGAAATGTTGTCCTCCTTGTAATTACAAATATTCCCTGGGAGCCAAAATCAAACTCAACAAGAAAAGCAACAGATTGTTCAACTAGAGGAAAAAACAAGAACTAAGAATAACTCAGCAAGAAAATACAAGAGATATCTTCTACTGTTTGGGTTATATTTTTATAAGATAGAGTAAACGTTCCAGATCAACACTTCAAAGATAAATCCACCACAAGTCTTAGAACAAACAATGTAAAGATAAACAAACACTGCCCAGCACAATTCGGAGGAGCTGGAATTCCCTCCACCTTCATAAGCCGATTATCCCAAAATTCACATAATCCCCAAAGCATCCGCAAAATCTCTCAAGTGGTCCCCTGTTGGTCCCCCTCAGCATAACCACATTCCTTCCACGTCCCACTATCTTCTCCTCTATGTGGAATTCCTTTCTTACCCTTTCGATAATATGTATAACGAATTGGGGGTTTCACAAGTTCCTCTTTTACCCTTCCTTGCATACGTGTGCTTAATCGGAGGGCTTACAGATTATTCATGAAAGAACCAAAAGTAAAATCATATAATATGGTATCAATTTCAAGCATGTATTGGTTATTACTCTGGATAGGAATACCTAGCTTCTACTTTCTTGGTTTCTCGCCTCATGGAATAGTGTTTTTCTTTGGGTGGGTAGGAACTTATGTAATGTTAAACTTCATGCATGGTTGGTTATTTCTCTGCTATTTTATCTTCTGTTGGTGGATAATAGTCGTTTGACTCAAAAAAGCCTTGGAGTTCTAGATAGCTTGATGAAATATCAATTCCCGGGAAATTGAAAGTTCTTCCTTTTTATCCTCCTTACTTTTCTAATTATTCCTGATATTGGCCTTTTTATTCCCTCTTTCTCTTCCATACTTCACTCTTTGCTTCGTTCTGTTCTACATCATTAAGCTACTTGGTGAGGATTAATCTTAACTTTTACGAAGTGCGACAAACCTTGTAGAGTAAGGATCTCAATATAATTCAATAATTGGTGAGCAAGTGATATAATTTGAGGAGGTTTCTCAACTTTTCCCTCAGGTAGGTATTGCCTTTCCTTCTGTTCTGGTCTGAAGACAGTGAGTTTTCATAGTAATGGTTTTAGACTTTATTTTGTCTTATATAGTTTTGACATTGTCACTATGCGAGTGTGCTGATTAACCTACATTCTTCGATGCTTATTCTGCCTTAGACAAACTTGCTATGCTATGAAGTTGAAAAGAAGTACTGTTTTCAGGCATATTCTGTCGGAAGAGCTTTGACTCAGAAGCTGAAGGAATTGATACCACGACAAATGTTTAAAGTACCTATTCAGGTAAAATTAATACTTCCTTCCTCAACGACATGATAACTATTGATCATATCATAACTTTATTATGTATTTTCCTGGTGTAAAAGTCTGAATTTCTTCCACTTTTCTTTTTCTACTTCTTCTTCATCATTTTTTTTCTCCTTTTATAAAAATAAATTAAAAAAAAACCTAGCAGTATTTTCAAGAACACATTTCAAATCCCAACCAAATGTAACAAAAAAGGTTCATACTGTTTACCTTTTTTATTTCTTTTTCAATGGCAATTAACATATTTACAGAGTCCCAGCATTACATTTGTAAGGTATCTTCCTTACGCTGTATTGATAATAGAAGAAGAAAACCTTTAAAGGCAGTACAAGATAGCTTCCAGCCAATTTAAAGGCTGGTAAACTCTCTTCCCTTCCCTGAACACTATTCACAGAATAAAAGCATAAATCGTAAGTCTACCCTCCCCAAGCATTGTACCTCTAGGCCTTGATTTTTCCTTCTTAGCCCTCTTGTATTGTATCAGAATTGGGGGTTGAACAACAGTGTCACCACATGATGTTATGGTTATTATTATGGTTGTAAAGTCATGAAACAAGTTGGGGCAAAATTCTTGGAGTAGCGTTGGTTATCAGAGAAAAATCGATCTCAACTCTATTGGCTTCAGGTGTTCACAAACTCAAATGTAGAGTTGGATATGGTGAATGCCTTAATGGCATTTGTAAACTGCAAAGGGGTTCCCTTTTCTCTTATCTTAATATGCCTCTTTAAAGAAATCCTTAGCATCTCCTAAAATCTTCGTCTCGAGAAGATATAATTTAGAATTCATAAGAACATGCCATTTTTTCAGAGGTAAAATACAGAAACCGGATTTTAGTTTCCTGTTCAGAATAGGATCGAGGACAGGAATAGGAGAAAATATTTTCCCCACAAGAATCTCCATTGCCTAAACGGAGATTCCTCTACCCTGAGTCTATTTCTCCAAATCAGGAAATGGGACAAGGATTGAGAATAAAAATCTATGCGCGCAAGGTTGGGGAGGGCATCCCTATCCGAGTTCCACCGTGCCATTTTCTACCAAGAGAATCAGTAAGCTTCCTTATGAAATTTTGTGAGAAGTAATTCTCATCTGATCATGCGGAGTGTCACAATCGTACTTGAAGTAAGTAGATTGTGACCCTGCTAATAAGTGGGGCGGACAGTGTAAGTGGCGACACGGGAGTGTCAACACATCTGGCAACGACAGAGGGCCAAGACAAGTGCCATGATGCGACCGAGGAGGACGGTGCATCATCCAACACACCAAGATAAATGGGCAAGCATGCCAAGTCGAGCATCAAGCGAGAATACAAGCATCAGTGCCATTGGCAGTGTCAGTGACAAGCTCAGTGGGGGCCAGATGGGGCCTCGGGCCGTTTCCTCAAAAAGAAGGAGTTTTGTTGTGAGTTTGGACGAGCGGCTTTGGAGTTAGAACCATATTGATGGAATATGGAAGCACGTCGAATTTCATGTCAATCGGAGTTCGGACGGGCGCTGTACAAAACTTGTGACGAGGTAGCACTGTCAACGTTCATCGGTGCCAAGGTGGGAAATGCTCAGAATGTACTATAAGGGGGAGGCATGGTGTCAGCTCTCCACCACCCCTTGGCCTGATGGCTTAAGCGAGCTACCCTATGGCACAGACATGTGCCATGGCGTGGGCGAGCGTTCTGGGACAAGAGTCTCGGGCGTCTTCCATTGAGAATGGGATCTCGTGGACAGTGTCGGACGGCACGGGTGTGCCGACATTGCGCTTTCTCCCATGGGCCGGAGGTTCGATATAGCACCCGCTATGCCGTTTTATCTCGGCAAATGATTTAGCATGGACACGGAAGGGTTCATGCCTCGTTATGTTTGGATGGATGGATGTCCGGGTAGTCCCGACGTTGGAAATATGCCCATGGGGTAGTTGTGACGATTCTTGACAAGCAGACCCGAGTTGGAGAGAGAAAGCTAATAGCCTTAGCCTCATGCAAAGGCAAGGTTGAGCTGTAATACTTGGCCTAGATTAAAGGCAAGTCGGATAGAGTCGCTTGATGGAGGGAAGTACACGTACAGGTAACGTGTATGTCTGAATAGAGTTCAGTTCCGCATGAGCTCAGTTCGGTTGAGGACGAGAGAACCTCGCCTAAGGTCCGTTGAGGCTTCAAAAAGAATGGAAAAAAGAAGATTGGGACTTGAGTTCCCCTTAAGGCTGACCATGTACGAGTGACTGTCATGGTGCCGCACGGTTGGAGAAGCCCGACCGTGACACGGAGCATGATCACCTTCTCTAAGGATCATAGGTTCAAATCTAAGTAGCACTCACTAATTTTGATTTATTGACAAATTTGACGCCTTCATGCTCCTCAGTGTCAACTTGAAAGGTAATTGTACAATTAGCGTTTTTACTTTATTTGTTCCTGTTAAATGGAATCTTGTTATTATTTTTTTTTTTAAAAAAAACATGTATAAAATTTTAAAAATTAATTAAAAAAATGTTTGGCATATGTAATGACTGAAATCACCCTCTTTGTTTTGTTTTCTATGGGGCACCCCTTTTCTTGGTTTTTTTTCCCCACCTGGCAGGCCTGTATTGGCTCTAAAGTGATTGCTAGTGAAACTATATCAGCAATTAGAAAGGATGTCCTTGCCAAATGCTACGGTGAGAAACTTTTCTTGCAGTTGAAGAATATCAATCTGTATTATTTCAGCATCAACTTTTATTGCTTTCTCTTACGTTTGAGGTCCAATCCTTTATTGATATCATTAAAAAGTTGACAATATTTTAGAGGGCAGCTGGTAAAATTTCTGGTGTTTGCTTTGTTTGTAGGTGGAGACATAACAAGGAAGAAGAAATTGCTTAGGAAACAGGTGTGTTGTGTTGTTTGACATTTCATTATTAGAAAATGAGACTACCTGTGGCTATGACTATCCATTTTCTTTTCCTAATTTGCCTTTACATTCACACTATCAATTTCTAATGTATAGGCTGAAGGAAAGAAGAGAATGAAAGCGATTGGGAGAGTTGATGTACCTCAAGAAGCTTTTATGGCTGTTTTGAAGCTTGAAAAGGAGGTTTTGTAATTTTGGTCCTTGGGATCATTTTTTTCCATTAACTATTTATTGTATATATTTTTCAAGTGACTCATATGACTTTTTTTTTCCCCTCCCAATAACTATGTTGTAATTTATGTATATAATTT

mRNA sequence

ATGACTTTTAGGTACAGAGAAAGTGAATTGATAAAACTGGACATTCAGATAAATGGTGATCGAGTGGAACCATTGGCAACAATAGTGCACAGCAATAAGACAAACTTGCTATGCTATGAAGTTGAAAAGAAGTACTGTTTTCAGGCATATTCTGTCGGAAGAGCTTTGACTCAGAAGCTGAAGGAATTGATACCACGACAAATGTTTAAAGTACCTATTCAGGCCTGTATTGGCTCTAAAGTGATTGCTAGTGAAACTATATCAGCAATTAGAAAGGATGTCCTTGCCAAATGCTACGGTGGAGACATAACAAGGAAGAAGAAATTGCTTAGGAAACAGGCTGAAGGAAAGAAGAGAATGAAAGCGATTGGGAGAGTTGATGTACCTCAAGAAGCTTTTATGGCTGTTTTGAAGCTTGAAAAGGAGGTTTTGTAATTTTGGTCCTTGGGATCATTTTTTTCCATTAACTATTTATTGTATATATTTTTCAAGTGACTCATATGACTTTTTTTTTCCCCTCCCAATAACTATGTTGTAATTTATGTATATAATTT

Coding sequence (CDS)

ATGACTTTTAGGTACAGAGAAAGTGAATTGATAAAACTGGACATTCAGATAAATGGTGATCGAGTGGAACCATTGGCAACAATAGTGCACAGCAATAAGACAAACTTGCTATGCTATGAAGTTGAAAAGAAGTACTGTTTTCAGGCATATTCTGTCGGAAGAGCTTTGACTCAGAAGCTGAAGGAATTGATACCACGACAAATGTTTAAAGTACCTATTCAGGCCTGTATTGGCTCTAAAGTGATTGCTAGTGAAACTATATCAGCAATTAGAAAGGATGTCCTTGCCAAATGCTACGGTGGAGACATAACAAGGAAGAAGAAATTGCTTAGGAAACAGGCTGAAGGAAAGAAGAGAATGAAAGCGATTGGGAGAGTTGATGTACCTCAAGAAGCTTTTATGGCTGTTTTGAAGCTTGAAAAGGAGGTTTTGTAA

Protein sequence

MTFRYRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Homology
BLAST of Lsi01G020220 vs. ExPASy Swiss-Prot
Match: A5B4D2 (Translation factor GUF1 homolog, chloroplastic OS=Vitis vinifera OX=29760 GN=VITISV_013255 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.9e-55
Identity = 114/140 (81.43%), Postives = 124/140 (88.57%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           Y+ESELIKLDIQING+RVEPLATIVH +K               AY+VGRALTQKLKELI
Sbjct: 556 YKESELIKLDIQINGERVEPLATIVHKDK---------------AYAVGRALTQKLKELI 615

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIG+KVIASE++SAIRKDVL+KCYGGDITRKKKLL+KQAEGKKRMKAIG
Sbjct: 616 PRQMFKVPIQACIGAKVIASESLSAIRKDVLSKCYGGDITRKKKLLKKQAEGKKRMKAIG 675

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           +VDVPQEAFMAVLKLEKEVL
Sbjct: 676 KVDVPQEAFMAVLKLEKEVL 680

BLAST of Lsi01G020220 vs. ExPASy Swiss-Prot
Match: B9GHA6 (Translation factor GUF1 homolog, chloroplastic OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_815670 PE=3 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-53
Identity = 112/140 (80.00%), Postives = 122/140 (87.14%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           Y+ES+LI+LDIQINGD VEPLATIVH +K               AYSVGRALTQKLKELI
Sbjct: 554 YKESDLIRLDIQINGDPVEPLATIVHKDK---------------AYSVGRALTQKLKELI 613

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIG+KVIASE++SAIRKDVLAKCYGGDI+RKKKLL+KQA GKKRMKAIG
Sbjct: 614 PRQMFKVPIQACIGAKVIASESLSAIRKDVLAKCYGGDISRKKKLLKKQAAGKKRMKAIG 673

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           +VDVPQEAFMAVLKLEKEVL
Sbjct: 674 KVDVPQEAFMAVLKLEKEVL 678

BLAST of Lsi01G020220 vs. ExPASy Swiss-Prot
Match: B8AI54 (Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_05919 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-52
Identity = 111/140 (79.29%), Postives = 120/140 (85.71%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRES L+KLDIQINGD VE L+TIVH +K               AYSVGRALTQKLKELI
Sbjct: 512 YRESNLVKLDIQINGDPVEALSTIVHRDK---------------AYSVGRALTQKLKELI 571

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMF+VPIQACIG+KVIASE +SAIRKDVL+KCYGGDI+RKKKLL+KQAEGKKRMKAIG
Sbjct: 572 PRQMFRVPIQACIGAKVIASEALSAIRKDVLSKCYGGDISRKKKLLKKQAEGKKRMKAIG 631

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 632 RVDVPQEAFMAVLKLEKEVL 636

BLAST of Lsi01G020220 vs. ExPASy Swiss-Prot
Match: B9F2U5 (Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0157700 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-52
Identity = 111/140 (79.29%), Postives = 120/140 (85.71%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRES L+KLDIQINGD VE L+TIVH +K               AYSVGRALTQKLKELI
Sbjct: 556 YRESNLVKLDIQINGDPVEALSTIVHRDK---------------AYSVGRALTQKLKELI 615

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMF+VPIQACIG+KVIASE +SAIRKDVL+KCYGGDI+RKKKLL+KQAEGKKRMKAIG
Sbjct: 616 PRQMFRVPIQACIGAKVIASEALSAIRKDVLSKCYGGDISRKKKLLKKQAEGKKRMKAIG 675

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 676 RVDVPQEAFMAVLKLEKEVL 680

BLAST of Lsi01G020220 vs. ExPASy Swiss-Prot
Match: Q9FNM5 (Translation factor GUF1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g08650 PE=1 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 4.6e-52
Identity = 111/140 (79.29%), Postives = 119/140 (85.00%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRES+LIKLDI IN + VEPL+TIVH +K               AYSVGRALTQKLKELI
Sbjct: 557 YRESDLIKLDILINAEMVEPLSTIVHRDK---------------AYSVGRALTQKLKELI 616

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASE +SAIRKDVLAKCYGGDI+RKKKLL+KQA GKKRMKAIG
Sbjct: 617 PRQMFKVPIQACIGSKVIASEALSAIRKDVLAKCYGGDISRKKKLLKKQAAGKKRMKAIG 676

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLE+EVL
Sbjct: 677 RVDVPQEAFMAVLKLEREVL 681

BLAST of Lsi01G020220 vs. ExPASy TrEMBL
Match: A0A6J1HRW4 (Translation factor GUF1 homolog, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466184 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.5e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 561 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 620

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 621 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 680

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 681 RVDVPQEAFMAVLKLEKEVL 685

BLAST of Lsi01G020220 vs. ExPASy TrEMBL
Match: A0A6J1EAZ6 (Translation factor GUF1 homolog, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111432406 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.5e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 561 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 620

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 621 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 680

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 681 RVDVPQEAFMAVLKLEKEVL 685

BLAST of Lsi01G020220 vs. ExPASy TrEMBL
Match: A0A5A7V5D5 (Translation factor GUF1 homolog, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold130G00180 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.5e-56
Identity = 123/140 (87.86%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPL+TIVH+NK               AYSVGRALTQKLKELI
Sbjct: 248 YRESELIKLDIQINGDRVEPLSTIVHNNK---------------AYSVGRALTQKLKELI 307

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 308 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 367

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 368 RVDVPQEAFMAVLKLEKEVL 372

BLAST of Lsi01G020220 vs. ExPASy TrEMBL
Match: A0A1S3BMN9 (Translation factor GUF1 homolog, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491317 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.5e-56
Identity = 123/140 (87.86%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPL+TIVH+NK               AYSVGRALTQKLKELI
Sbjct: 558 YRESELIKLDIQINGDRVEPLSTIVHNNK---------------AYSVGRALTQKLKELI 617

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 618 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 677

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 678 RVDVPQEAFMAVLKLEKEVL 682

BLAST of Lsi01G020220 vs. ExPASy TrEMBL
Match: A0A0A0KYH8 (Translation factor GUF1 homolog, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_4G437010 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 7.2e-56
Identity = 122/140 (87.14%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPL+TIVH+NK               AYSVGRALTQKLKELI
Sbjct: 251 YRESELIKLDIQINGDRVEPLSTIVHNNK---------------AYSVGRALTQKLKELI 310

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSK+IASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 311 PRQMFKVPIQACIGSKIIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 370

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 371 RVDVPQEAFMAVLKLEKEVL 375

BLAST of Lsi01G020220 vs. NCBI nr
Match: XP_022925039.1 (translation factor GUF1 homolog, chloroplastic [Cucurbita moschata])

HSP 1 Score: 228.0 bits (580), Expect = 5.1e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 561 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 620

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 621 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 680

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 681 RVDVPQEAFMAVLKLEKEVL 685

BLAST of Lsi01G020220 vs. NCBI nr
Match: KAG6595718.1 (Translation factor GUF1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 228.0 bits (580), Expect = 5.1e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 561 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 620

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 621 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 680

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 681 RVDVPQEAFMAVLKLEKEVL 685

BLAST of Lsi01G020220 vs. NCBI nr
Match: XP_023518730.1 (translation factor GUF1 homolog, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 228.0 bits (580), Expect = 5.1e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 561 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 620

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 621 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 680

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 681 RVDVPQEAFMAVLKLEKEVL 685

BLAST of Lsi01G020220 vs. NCBI nr
Match: XP_022966535.1 (translation factor GUF1 homolog, chloroplastic [Cucurbita maxima])

HSP 1 Score: 228.0 bits (580), Expect = 5.1e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 561 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 620

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 621 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 680

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 681 RVDVPQEAFMAVLKLEKEVL 685

BLAST of Lsi01G020220 vs. NCBI nr
Match: KAG7027680.1 (Translation factor GUF1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 228.0 bits (580), Expect = 5.1e-56
Identity = 124/140 (88.57%), Postives = 125/140 (89.29%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELI
Sbjct: 252 YRESELIKLDIQINGDRVEPLATIVHNNK---------------AYSVGRALTQKLKELI 311

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG
Sbjct: 312 PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 371

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLEKEVL
Sbjct: 372 RVDVPQEAFMAVLKLEKEVL 376

BLAST of Lsi01G020220 vs. TAIR 10
Match: AT5G08650.1 (Small GTP-binding protein )

HSP 1 Score: 205.3 bits (521), Expect = 3.3e-53
Identity = 111/140 (79.29%), Postives = 119/140 (85.00%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           YRES+LIKLDI IN + VEPL+TIVH +K               AYSVGRALTQKLKELI
Sbjct: 557 YRESDLIKLDILINAEMVEPLSTIVHRDK---------------AYSVGRALTQKLKELI 616

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
           PRQMFKVPIQACIGSKVIASE +SAIRKDVLAKCYGGDI+RKKKLL+KQA GKKRMKAIG
Sbjct: 617 PRQMFKVPIQACIGSKVIASEALSAIRKDVLAKCYGGDISRKKKLLKKQAAGKKRMKAIG 676

Query: 125 RVDVPQEAFMAVLKLEKEVL 145
           RVDVPQEAFMAVLKLE+EVL
Sbjct: 677 RVDVPQEAFMAVLKLEREVL 681

BLAST of Lsi01G020220 vs. TAIR 10
Match: AT5G39900.1 (Small GTP-binding protein )

HSP 1 Score: 152.9 bits (385), Expect = 1.9e-37
Identity = 81/135 (60.00%), Postives = 97/135 (71.85%), Query Frame = 0

Query: 5   YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELI 64
           Y+ S+L+KLDI +NG  V+ LATIVH  K               AY VG+ L +KLK  I
Sbjct: 543 YQASDLVKLDILLNGQAVDALATIVHKQK---------------AYRVGKELVEKLKNYI 602

Query: 65  PRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLLRKQAEGKKRMKAIG 124
            RQMF+V IQA IGSK+IA +TISA+RK+VLAKCYGGDITRKKKLL KQ EGKKRMK +G
Sbjct: 603 ERQMFEVMIQAAIGSKIIARDTISAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKRVG 662

Query: 125 RVDVPQEAFMAVLKL 140
            VD+P EAF  +LK+
Sbjct: 663 SVDIPHEAFQQILKV 662

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A5B4D25.9e-5581.43Translation factor GUF1 homolog, chloroplastic OS=Vitis vinifera OX=29760 GN=VIT... [more]
B9GHA61.9e-5380.00Translation factor GUF1 homolog, chloroplastic OS=Populus trichocarpa OX=3694 GN... [more]
B8AI541.2e-5279.29Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. indica OX=... [more]
B9F2U51.2e-5279.29Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. japonica O... [more]
Q9FNM54.6e-5279.29Translation factor GUF1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1HRW42.5e-5688.57Translation factor GUF1 homolog, chloroplastic OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1EAZ62.5e-5688.57Translation factor GUF1 homolog, chloroplastic OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5A7V5D55.5e-5687.86Translation factor GUF1 homolog, mitochondrial OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BMN95.5e-5687.86Translation factor GUF1 homolog, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KYH87.2e-5687.14Translation factor GUF1 homolog, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa... [more]
Match NameE-valueIdentityDescription
XP_022925039.15.1e-5688.57translation factor GUF1 homolog, chloroplastic [Cucurbita moschata][more]
KAG6595718.15.1e-5688.57Translation factor GUF1-like, chloroplastic, partial [Cucurbita argyrosperma sub... [more]
XP_023518730.15.1e-5688.57translation factor GUF1 homolog, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022966535.15.1e-5688.57translation factor GUF1 homolog, chloroplastic [Cucurbita maxima][more]
KAG7027680.15.1e-5688.57Translation factor GUF1-like, chloroplastic, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT5G08650.13.3e-5379.29Small GTP-binding protein [more]
AT5G39900.11.9e-3760.00Small GTP-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013842GTP-binding protein LepA, C-terminalPFAMPF06421LepA_Ccoord: 17..138
e-value: 1.0E-43
score: 147.8
IPR038363LepA, C-terminal domain superfamilyGENE3D3.30.70.2570coord: 44..98
e-value: 3.2E-22
score: 80.3
IPR006297Elongation factor 4PANTHERPTHR43512TRANSLATION FACTOR GUF1-RELATEDcoord: 4..140
NoneNo IPR availablePANTHERPTHR43512:SF6TRANSLATION FACTOR GUF1 HOMOLOG, MITOCHONDRIALcoord: 4..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G020220.1Lsi01G020220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045727 positive regulation of translation
biological_process GO:0006414 translational elongation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0043022 ribosome binding
molecular_function GO:0003746 translation elongation factor activity