Lsi01G019770 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G019770
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Proteinase inhibitor 1
Location	chr01: 26088610 .. 26089359 (-)
RNA-Seq Expression	Lsi01G019770
Synteny	Lsi01G019770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTAAGATACAAAGAGAAGCTCTCAGTAATTAGAAAAAGACATGGCTGATATTCCTTTCCCTATTCCTGGTAAGCCTAATTCAAAACCTTTACATCACTTCTCTAAACTAACAACATTTGAAAATTTTGAATCTCATTTTTAAATATATTTATAAATTGAATTGTTTTTTTATAGAAGTTATTACGTTAAAGATCAATATTTGGAGATGAATTGTAGAACTTGTTATAGTGTTAATGGTTGATTGAACATAAAAGATCATACTATTTATTCACTTTTGTCAAACAAATGGCAAATGATGTTTTCGTTTTTAGAAGTGTTTTCATTTAGTTTAATATTTGGAGATGAATCGTAGAACTTGTTATTTATATACTACTAATTTGTTGTAAAGACTTGTTTGGTAGCCAATCTAAAAATATAAATTCAGATTGATTATCAAACACATATTTGTTAAACTAAGTGAATCTGAAAACACGAAACAATATTCAAATTGCCAACTAAATAAATCCTAAATTATTTGTACTAATTAATGAAATATTATTTTGATCCATTAGGAAAATCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

mRNA sequence

CCTTAAGATACAAAGAGAAGCTCTCAGTAATTAGAAAAAGACATGGCTGATATTCCTTTCCCTATTCCTGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

Coding sequence (CDS)

ATGGCTGATATTCCTTTCCCTATTCCTGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

Protein sequence

MADIPFPIPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG

Homology

[Close]

BLAST of Lsi01G019770 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.6e-10
Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIP 66
           PEL+G+ A  AK +I+K+NP +  I I+L GSPV +DFR DRVR+FVNI    V+IP
Sbjct: 52  PELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIP 108

[Close]

BLAST of Lsi01G019770 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.5e-10
Identity = 28/60 (46.67%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A+ A+ +IQK+N ++  +  +L GSPV +DFR +RVR+FVN+ +  V+IP +G
Sbjct: 35 PELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIPRVG 94

[Close]

BLAST of Lsi01G019770 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.8e-09
Identity = 28/60 (46.67%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+  ++AK +I+K+N  +  + I+L GSPV  D R DRVR+F NI    V+IP++G
Sbjct: 48  PELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVDIPVVG 107

[Close]

BLAST of Lsi01G019770 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 3.0e-09
Identity = 26/59 (44.07%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPII 68
           PEL+G+  ++AK +I+K+NP +  I I+L+GSP+  D+  DRVR+F NI    V++P++
Sbjct: 52  PELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQMPVV 110

[Close]

BLAST of Lsi01G019770 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-08
Identity = 26/60 (43.33%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G  A+ AK +IQK+NP++  ++ +L GS    D R +RVR+FVN+ ++ V+ P +G
Sbjct: 60  PELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTPKVG 119

[Close]

BLAST of Lsi01G019770 vs. ExPASy TrEMBL
Match: A0A6N2B0Y4 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_018437 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.4e-09
Identity = 34/60 (56.67%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+ A+ AK +IQK+NP +  + I+L GSPV  DFR  RVRI VNI +VAV IP++G
Sbjct: 52  PELIGVPAQYAKGIIQKENPFITDVQIVLNGSPVTADFRCFRVRIVVNILDVAVSIPVVG 111

[Close]

BLAST of Lsi01G019770 vs. ExPASy TrEMBL
Match: Q40444 (Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii OX=79230 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.3e-09
Identity = 30/60 (50.00%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A+ A+ +IQK+NP++  +  +L GSPV +DFR +RVR+FVN+ +V V+IP +G
Sbjct: 5  PELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQIPRVG 64

[Close]

BLAST of Lsi01G019770 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 6.9e-09
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 1  MADIPFP----IPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVN 60
          MADI  P     PELVG++A  AK +I+KDNP VE + ++LAGS    D R DRV +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IRNVAVEIPIIG 69
          I ++ VE+P +G
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of Lsi01G019770 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 9.1e-09
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 1  MADIPFP----IPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVN 60
          MADI  P     PELVGI+A IA+ +I+KDNP VE + ++LAG+   +DFR DRV +F N
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IRNVAVEIPIIG 69
          I    VE+P +G
Sbjct: 61 IDGRVVEMPTVG 72

[Close]

BLAST of Lsi01G019770 vs. ExPASy TrEMBL
Match: A0A2G2VTY8 (Ethylene-responsive proteinase inhibitor 1 OS=Capsicum baccatum OX=33114 GN=CQW23_24145 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 9.1e-09
Identity = 31/60 (51.67%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+ A +A+  IQK+NP++  +  +L GSPV RDFR DRVR+FVN+ +V V+ P +G
Sbjct: 48  PELLGVPAGLARRTIQKENPKLTNVPSVLNGSPVTRDFRCDRVRLFVNVLDVVVQTPRVG 107

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: KAG6571499.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 74.7 bits (182), Expect = 3.4e-10
Identity = 37/60 (61.67%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PELVGIEA I + +I+K+NP V I+ I LAGSPVP DF   RVR+FVNI +V V IP  G
Sbjct: 13 PELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVSIPWTG 72

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: XP_004247658.1 (wound-induced proteinase inhibitor 1 [Solanum lycopersicum])

HSP 1 Score: 73.2 bits (178), Expect = 9.9e-10
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+ A+ AK +IQK+NP V  + I+L GSPV  DFR  RVRI VNI NVAV IP++G
Sbjct: 52  PELIGVPAQYAKGIIQKENPFVTDVQIVLNGSPVTADFRCFRVRIVVNILNVAVSIPVVG 111

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-09
Identity = 36/60 (60.00%), Postives = 46/60 (76.67%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PELVGI++ IA+ +I+KDN  VE ++ ILAGS V  D R DRVRIFVNI+ + V+IP IG
Sbjct: 13 PELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKGIIVQIPTIG 72

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: TMW88525.1 (hypothetical protein EJD97_018437 [Solanum chilense])

HSP 1 Score: 70.9 bits (172), Expect = 4.9e-09
Identity = 34/60 (56.67%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+ A+ AK +IQK+NP +  + I+L GSPV  DFR  RVRI VNI +VAV IP++G
Sbjct: 52  PELIGVPAQYAKGIIQKENPFITDVQIVLNGSPVTADFRCFRVRIVVNILDVAVSIPVVG 111

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: BAA05472.1 (tumor-related protein, partial [Nicotiana glauca x Nicotiana langsdorffii])

HSP 1 Score: 69.7 bits (169), Expect = 1.1e-08
Identity = 30/60 (50.00%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A+ A+ +IQK+NP++  +  +L GSPV +DFR +RVR+FVN+ +V V+IP +G
Sbjct: 5  PELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQIPRVG 64

[Close]

BLAST of Lsi01G019770 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.3 bits (129), Expect = 4.4e-08
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL G   + A +VI+++NP V    +IL GSPV  DFR DRVR+FV+   + V+ P  G
Sbjct: 12 PELTGTNGDYAAVVIERENPTVNAA-VILDGSPVTADFRCDRVRVFVDGNRIVVKTPKSG 70

[Close]

BLAST of Lsi01G019770 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-07
Identity = 28/60 (46.67%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G + E AK VI+++NP+++ + IIL G+ VP  F   RV ++VN   + V+IPIIG
Sbjct: 41 PELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIVVQIPIIG 99

The following BLAST results are available for this feature:

BLAST of Lsi01G019770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	1.6e-10	56.14	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q02214	3.5e-10	46.67	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
Q00783	1.8e-09	46.67	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P05118	3.0e-09	44.07	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
P20076	1.9e-08	43.33	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]

BLAST of Lsi01G019770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6N2B0Y4	2.4e-09	56.67	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_018437 PE=3 SV=1	[more]
Q40444	5.3e-09	50.00	Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii OX...	[more]
A0A0A0KME7	6.9e-09	51.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A0A0KMM0	9.1e-09	51.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
A0A2G2VTY8	9.1e-09	51.67	Ethylene-responsive proteinase inhibitor 1 OS=Capsicum baccatum OX=33114 GN=CQW2...	[more]

BLAST of Lsi01G019770 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571499.1	3.4e-10	61.67	Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004247658.1	9.9e-10	60.00	wound-induced proteinase inhibitor 1 [Solanum lycopersicum]	[more]
KAG6571494.1	1.3e-09	60.00	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
TMW88525.1	4.9e-09	56.67	hypothetical protein EJD97_018437 [Solanum chilense]	[more]
BAA05472.1	1.1e-08	50.00	tumor-related protein, partial [Nicotiana glauca x Nicotiana langsdorffii]	[more]

BLAST of Lsi01G019770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	4.4e-08	46.67	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.7e-07	46.67	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 45..55 score: 59.29 coord: 7..20 score: 47.52 coord: 33..44 score: 38.04
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..68 e-value: 5.1E-19 score: 68.7
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 9..68
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 8..68 e-value: 5.2E-18 score: 67.0
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 9..68
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 9..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G019770.1	Lsi01G019770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity