Lsi01G013960 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G013960
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr01: 12110088 .. 12111638 (-)
RNA-Seq ExpressionLsi01G013960
SyntenyLsi01G013960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCATAACAATTTGGAGAAAATCTTTCACTTCATCTTGTAGCCAATTGATCAAACTCAAAGCCGATGGCCATGGCGCCGACGGTACTCCGCCTCTTCTATCCAAGACAGCCCCATTTTCTCGTGGTGAATCGAATGCCCGCCAACTGTTCGACAAAATGCCTCACTTAAACGTTGTTTCAGCAACCGCCGAGATTGGGCGCTGCGCAAGGCAGCACCAATACGAAGAGGCATTATGTCTCTTTTCTGCAATGTTTGTGTTAAATTTAAGGCCCAACGAGTTCACTTTTGGAACTGTGATTCATTCTGCAACTGCACTTAGAGACATTGATATTGGCAAGCAACTTCATATTTGTGCCATAAAAACAGGCTTTCATTCTAATGTGTTTGTTGGTAGTGCGCTTTTGGATTTGTATGTCAAGTTGAGTGTCATTGAGGAAGCTCAAAGAGCTTTTGATGATATCAAAATGCCAAATGTGGTTTCTTACACTAGTTTGATTTCTGGGTACCTGAAAATGGAGAGGATTCAAGATGCTCTCCAGGTGTTCGAGGAAATGCCCGAGCGAAACGTCGTGTCATGGAATGCGATGATTGGCGGATTTAGCCAAAAGGGACACAATGAGGATGCTGTGCATCTGTTCATTGATATGCTTAGAGAAGGGTTTTTGCCCACTCAGTCTACTTTTCCTTGTGCTATATGTGCTGCTGCTAATATAGCATCTATTGGGATAGGGAGGAGTTTTCATGCTTGTGCTGTCAAGTTCTTGGGCAAGCTCGACGTGTTCGTTAGCAATTCGCTTATTAGTTTCTATGCAAAATGTGGAAGTATGGAAGATAGTTTGTTGGTTTTCAATAAATTACTTGATGAAAGAAACATAGTTTCATGGAATGCTGTCCTTTGTGGTTATGCTCAAAATGGTAGGGGAAAGGAAGCTATAGATTTCTATCAAAAGATGATTTTTGCAGGCTGCAAACCAAATGATGTGACACTTCTTAGCTTATTATGGGCTTGTAATCATGCTGGCCTGGTTGATGAAGGTTATTCATATTTCAATCAAGCGAAAATCGATAACCCCAGCCTGCTAAAACCCGAGCATTATGCTTGTATGGTCGATTTGCTTTCACGTTCGGGTCAGTTGAGACGAGCAGAGGAATTTATACGTGACCTGCCATTCGATCCAGGGATCGGGTTTTGGAAAGCATTGCTCGGGGGCTGCCAAATTCACTTAAATGTGGAACTTGGGGAGTTTGCAGCTCAGAGAATTCTGGCCTTGGATCCGGGTGATGTATCGTCGTATGTAATGATGTCGAATGCGCATTCTGCAGCCGGGAAATGGCAAAGCATGTCGATATTAAGAAGGGAAATGAAGGAGAAGGGGATGAAGAGAATTCCAGGTTGCAGTTGGATTGAAATTAGAAGCAAAGTTCATGTATTTGTTACTGGTGATAAGAATCACCACCAAAAGGATGAAATTTATTCTGCCTTGAGATTCTTTGTTGAGCACCAGAAAGAGAGTGAAGATTTCAACTTTCTTTCAAATTCATAA

mRNA sequence

ATGAAACCCATAACAATTTGGAGAAAATCTTTCACTTCATCTTGTAGCCAATTGATCAAACTCAAAGCCGATGGCCATGGCGCCGACGGTACTCCGCCTCTTCTATCCAAGACAGCCCCATTTTCTCGTGGTGAATCGAATGCCCGCCAACTGTTCGACAAAATGCCTCACTTAAACGTTGTTTCAGCAACCGCCGAGATTGGGCGCTGCGCAAGGCAGCACCAATACGAAGAGGCATTATGTCTCTTTTCTGCAATGTTTGTGTTAAATTTAAGGCCCAACGAGTTCACTTTTGGAACTGTGATTCATTCTGCAACTGCACTTAGAGACATTGATATTGGCAAGCAACTTCATATTTGTGCCATAAAAACAGGCTTTCATTCTAATGTGTTTGTTGGTAGTGCGCTTTTGGATTTGTATGTCAAGTTGAGTGTCATTGAGGAAGCTCAAAGAGCTTTTGATGATATCAAAATGCCAAATGTGGTTTCTTACACTAGTTTGATTTCTGGGTACCTGAAAATGGAGAGGATTCAAGATGCTCTCCAGGTGTTCGAGGAAATGCCCGAGCGAAACGTCGTGTCATGGAATGCGATGATTGGCGGATTTAGCCAAAAGGGACACAATGAGGATGCTGTGCATCTGTTCATTGATATGCTTAGAGAAGGGTTTTTGCCCACTCAGTCTACTTTTCCTTGTGCTATATGTGCTGCTGCTAATATAGCATCTATTGGGATAGGGAGGAGTTTTCATGCTTGTGCTGTCAAGTTCTTGGGCAAGCTCGACGTGTTCGTTAGCAATTCGCTTATTAGTTTCTATGCAAAATGTGGAAGTATGGAAGATAGTTTGTTGGTTTTCAATAAATTACTTGATGAAAGAAACATAGTTTCATGGAATGCTGTCCTTTGTGGTTATGCTCAAAATGGTAGGGGAAAGGAAGCTATAGATTTCTATCAAAAGATGATTTTTGCAGGCTGCAAACCAAATGATGTGACACTTCTTAGCTTATTATGGGCTTGTAATCATGCTGGCCTGGTTGATGAAGGTTATTCATATTTCAATCAAGCGAAAATCGATAACCCCAGCCTGCTAAAACCCGAGCATTATGCTTGTATGGTCGATTTGCTTTCACGTTCGGGTCAGTTGAGACGAGCAGAGGAATTTATACGTGACCTGCCATTCGATCCAGGGATCGGGTTTTGGAAAGCATTGCTCGGGGGCTGCCAAATTCACTTAAATGTGGAACTTGGGGAGTTTGCAGCTCAGAGAATTCTGGCCTTGGATCCGGGTGATGTATCGTCGTATGTAATGATGTCGAATGCGCATTCTGCAGCCGGGAAATGGCAAAGCATGTCGATATTAAGAAGGGAAATGAAGGAGAAGGGGATGAAGAGAATTCCAGGTTGCAGTTGGATTGAAATTAGAAGCAAAGTTCATGTATTTGTTACTGGTGATAAGAATCACCACCAAAAGGATGAAATTTATTCTGCCTTGAGATTCTTTGTTGAGCACCAGAAAGAGAGTGAAGATTTCAACTTTCTTTCAAATTCATAA

Coding sequence (CDS)

ATGAAACCCATAACAATTTGGAGAAAATCTTTCACTTCATCTTGTAGCCAATTGATCAAACTCAAAGCCGATGGCCATGGCGCCGACGGTACTCCGCCTCTTCTATCCAAGACAGCCCCATTTTCTCGTGGTGAATCGAATGCCCGCCAACTGTTCGACAAAATGCCTCACTTAAACGTTGTTTCAGCAACCGCCGAGATTGGGCGCTGCGCAAGGCAGCACCAATACGAAGAGGCATTATGTCTCTTTTCTGCAATGTTTGTGTTAAATTTAAGGCCCAACGAGTTCACTTTTGGAACTGTGATTCATTCTGCAACTGCACTTAGAGACATTGATATTGGCAAGCAACTTCATATTTGTGCCATAAAAACAGGCTTTCATTCTAATGTGTTTGTTGGTAGTGCGCTTTTGGATTTGTATGTCAAGTTGAGTGTCATTGAGGAAGCTCAAAGAGCTTTTGATGATATCAAAATGCCAAATGTGGTTTCTTACACTAGTTTGATTTCTGGGTACCTGAAAATGGAGAGGATTCAAGATGCTCTCCAGGTGTTCGAGGAAATGCCCGAGCGAAACGTCGTGTCATGGAATGCGATGATTGGCGGATTTAGCCAAAAGGGACACAATGAGGATGCTGTGCATCTGTTCATTGATATGCTTAGAGAAGGGTTTTTGCCCACTCAGTCTACTTTTCCTTGTGCTATATGTGCTGCTGCTAATATAGCATCTATTGGGATAGGGAGGAGTTTTCATGCTTGTGCTGTCAAGTTCTTGGGCAAGCTCGACGTGTTCGTTAGCAATTCGCTTATTAGTTTCTATGCAAAATGTGGAAGTATGGAAGATAGTTTGTTGGTTTTCAATAAATTACTTGATGAAAGAAACATAGTTTCATGGAATGCTGTCCTTTGTGGTTATGCTCAAAATGGTAGGGGAAAGGAAGCTATAGATTTCTATCAAAAGATGATTTTTGCAGGCTGCAAACCAAATGATGTGACACTTCTTAGCTTATTATGGGCTTGTAATCATGCTGGCCTGGTTGATGAAGGTTATTCATATTTCAATCAAGCGAAAATCGATAACCCCAGCCTGCTAAAACCCGAGCATTATGCTTGTATGGTCGATTTGCTTTCACGTTCGGGTCAGTTGAGACGAGCAGAGGAATTTATACGTGACCTGCCATTCGATCCAGGGATCGGGTTTTGGAAAGCATTGCTCGGGGGCTGCCAAATTCACTTAAATGTGGAACTTGGGGAGTTTGCAGCTCAGAGAATTCTGGCCTTGGATCCGGGTGATGTATCGTCGTATGTAATGATGTCGAATGCGCATTCTGCAGCCGGGAAATGGCAAAGCATGTCGATATTAAGAAGGGAAATGAAGGAGAAGGGGATGAAGAGAATTCCAGGTTGCAGTTGGATTGAAATTAGAAGCAAAGTTCATGTATTTGTTACTGGTGATAAGAATCACCACCAAAAGGATGAAATTTATTCTGCCTTGAGATTCTTTGTTGAGCACCAGAAAGAGAGTGAAGATTTCAACTTTCTTTCAAATTCATAA

Protein sequence

MKPITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS
Homology
BLAST of Lsi01G013960 vs. ExPASy Swiss-Prot
Match: Q9FIH2 (Pentatricopeptide repeat-containing protein At5g42450, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E102 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.0e-165
Identity = 282/468 (60.26%), Postives = 364/468 (77.78%), Query Frame = 0

Query: 47  NARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSAT 106
           NA ++FD++P L+V+SATA IGR  ++ ++ EA   F  +  L +RPNEFTFGTVI S+T
Sbjct: 45  NAHKVFDEIPELDVISATAVIGRFVKESRHVEASQAFKRLLCLGIRPNEFTFGTVIGSST 104

Query: 107 ALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTS 166
             RD+ +GKQLH  A+K G  SNVFVGSA+L+ YVKLS + +A+R FDD + PNVVS T+
Sbjct: 105 TSRDVKLGKQLHCYALKMGLASNVFVGSAVLNCYVKLSTLTDARRCFDDTRDPNVVSITN 164

Query: 167 LISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREG-FLP 226
           LISGYLK    ++AL +F  MPER+VV+WNA+IGGFSQ G NE+AV+ F+DMLREG  +P
Sbjct: 165 LISGYLKKHEFEEALSLFRAMPERSVVTWNAVIGGFSQTGRNEEAVNTFVDMLREGVVIP 224

Query: 227 TQSTFPCAICAAANIASIGIGRSFHACAVKFLGK-LDVFVSNSLISFYAKCGSMEDSLLV 286
            +STFPCAI A +NIAS G G+S HACA+KFLGK  +VFV NSLISFY+KCG+MEDSLL 
Sbjct: 225 NESTFPCAITAISNIASHGAGKSIHACAIKFLGKRFNVFVWNSLISFYSKCGNMEDSLLA 284

Query: 287 FNKLLDE-RNIVSWNAVLCGYAQNGRGKEAIDFYQKMI-FAGCKPNDVTLLSLLWACNHA 346
           FNKL +E RNIVSWN+++ GYA NGRG+EA+  ++KM+     +PN+VT+L +L+ACNHA
Sbjct: 285 FNKLEEEQRNIVSWNSMIWGYAHNGRGEEAVAMFEKMVKDTNLRPNNVTILGVLFACNHA 344

Query: 347 GLVDEGYSYFNQA--KIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFW 406
           GL+ EGY YFN+A    D+P+LL+ EHYACMVD+LSRSG+ + AEE I+ +P DPGIGFW
Sbjct: 345 GLIQEGYMYFNKAVNDYDDPNLLELEHYACMVDMLSRSGRFKEAEELIKSMPLDPGIGFW 404

Query: 407 KALLGGCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEK 466
           KALLGGCQIH N  L + AA +IL LDP DVSSYVM+SNA+SA   WQ++S++RR+MKE 
Sbjct: 405 KALLGGCQIHSNKRLAKLAASKILELDPRDVSSYVMLSNAYSAMENWQNVSLIRRKMKET 464

Query: 467 GMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESE 509
           G+KR  GCSWIE+R ++ VFV  DKN+  KDE+Y  L    +H +E+E
Sbjct: 465 GLKRFTGCSWIEVRDQIRVFVNADKNNELKDEVYRMLALVSQHLEENE 512

BLAST of Lsi01G013960 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.4e-98
Identity = 177/471 (37.58%), Postives = 289/471 (61.36%), Query Frame = 0

Query: 46  SNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSA 105
           ++A+++FD+M   NVVS  + I    +     EAL +F  M    + P+E T  +VI + 
Sbjct: 204 NDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISAC 263

Query: 106 TALRDIDIGKQLHICAIKTG-FHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSY 165
            +L  I +G+++H   +K     +++ + +A +D+Y K S I+EA+  FD + + NV++ 
Sbjct: 264 ASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAE 323

Query: 166 TSLISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFL 225
           TS+ISGY      + A  +F +M ERNVVSWNA+I G++Q G NE+A+ LF  + RE   
Sbjct: 324 TSMISGYAMAASTKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVC 383

Query: 226 PTQSTFPCAICAAANIASIGIGRSFHACAVKFLGKL------DVFVSNSLISFYAKCGSM 285
           PT  +F   + A A++A + +G   H   +K   K       D+FV NSLI  Y KCG +
Sbjct: 384 PTHYSFANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCV 443

Query: 286 EDSLLVFNKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWA 345
           E+  LVF K++ ER+ VSWNA++ G+AQNG G EA++ +++M+ +G KP+ +T++ +L A
Sbjct: 444 EEGYLVFRKMM-ERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSA 503

Query: 346 CNHAGLVDEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIG 405
           C HAG V+EG  YF+    D       +HY CMVDLL R+G L  A+  I ++P  P   
Sbjct: 504 CGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSV 563

Query: 406 FWKALLGGCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMK 465
            W +LL  C++H N+ LG++ A+++L ++P +   YV++SN ++  GKW+ +  +R+ M+
Sbjct: 564 IWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMR 623

Query: 466 EKGMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESED 510
           ++G+ + PGCSWI+I+   HVF+  DK+H +K +I+S L   +   +  +D
Sbjct: 624 KEGVTKQPGCSWIKIQGHDHVFMVKDKSHPRKKQIHSLLDILIAEMRPEQD 673

BLAST of Lsi01G013960 vs. ExPASy Swiss-Prot
Match: Q9FWA6 (Pentatricopeptide repeat-containing protein At3g02330, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E90 PE=2 SV=2)

HSP 1 Score: 343.6 bits (880), Expect = 3.9e-93
Identity = 176/473 (37.21%), Postives = 279/473 (58.99%), Query Frame = 0

Query: 46  SNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSA 105
           + A ++FD+M   + VS  A I    +  +  E L LF +M    + P+EFTFG+++ + 
Sbjct: 434 AEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSRIEPDEFTFGSILKAC 493

Query: 106 TALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYT 165
           T    +  G ++H   +K+G  SN  VG +L+D+Y K  +IEEA++              
Sbjct: 494 TG-GSLGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEK-------------- 553

Query: 166 SLISGYLKMERIQDALQVFEEMPERNV----VSWNAMIGGFSQKGHNEDAVHLFIDMLRE 225
            + S + +   +   ++  E+M  + +    VSWN++I G+  K  +EDA  LF  M+  
Sbjct: 554 -IHSRFFQRANVSGTMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRMMEM 613

Query: 226 GFLPTQSTFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDS 285
           G  P + T+   +   AN+AS G+G+  HA  +K   + DV++ ++L+  Y+KCG + DS
Sbjct: 614 GITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDLHDS 673

Query: 286 LLVFNKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNH 345
            L+F K L  R+ V+WNA++CGYA +G+G+EAI  +++MI    KPN VT +S+L AC H
Sbjct: 674 RLMFEKSL-RRDFVTWNAMICGYAHHGKGEEAIQLFERMILENIKPNHVTFISILRACAH 733

Query: 346 AGLVDEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWK 405
            GL+D+G  YF   K D     +  HY+ MVD+L +SG+++RA E IR++PF+     W+
Sbjct: 734 MGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADDVIWR 793

Query: 406 ALLGGCQIHL-NVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEK 465
            LLG C IH  NVE+ E A   +L LDP D S+Y ++SN ++ AG W+ +S LRR M+  
Sbjct: 794 TLLGVCTIHRNNVEVAEEATAALLRLDPQDSSAYTLLSNVYADAGMWEKVSDLRRNMRGF 853

Query: 466 GMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESEDFNFL 514
            +K+ PGCSW+E++ ++HVF+ GDK H + +EIY  L       K  +D +F+
Sbjct: 854 KLKKEPGCSWVELKDELHVFLVGDKAHPRWEEIYEELGLIYSEMKPFDDSSFV 889

BLAST of Lsi01G013960 vs. ExPASy Swiss-Prot
Match: Q9LIQ7 (Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H87 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 7.4e-92
Identity = 173/448 (38.62%), Postives = 266/448 (59.38%), Query Frame = 0

Query: 48  ARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATA 107
           AR++F+KMP  + V+ T  I   ++  +  +AL  F+ M      PNEFT  +VI +A A
Sbjct: 114 ARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAA 173

Query: 108 LRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSL 167
            R    G QLH   +K GF SNV VGSALLDLY +  ++++AQ  FD ++          
Sbjct: 174 ERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALE---------- 233

Query: 168 ISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQ 227
                                 RN VSWNA+I G +++   E A+ LF  MLR+GF P+ 
Sbjct: 234 ---------------------SRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSH 293

Query: 228 STFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNK 287
            ++     A ++   +  G+  HA  +K   KL  F  N+L+  YAK GS+ D+  +F++
Sbjct: 294 FSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDR 353

Query: 288 LLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDE 347
           L  +R++VSWN++L  YAQ+G GKEA+ ++++M   G +PN+++ LS+L AC+H+GL+DE
Sbjct: 354 LA-KRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSHSGLLDE 413

Query: 348 GYSYFNQAKIDNPSLLKPE--HYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLG 407
           G+ Y+   K D    + PE  HY  +VDLL R+G L RA  FI ++P +P    WKALL 
Sbjct: 414 GWHYYELMKKDG---IVPEAWHYVTVVDLLGRAGDLNRALRFIEEMPIEPTAAIWKALLN 473

Query: 408 GCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRI 467
            C++H N ELG +AA+ +  LDP D   +V++ N +++ G+W   + +R++MKE G+K+ 
Sbjct: 474 ACRMHKNTELGAYAAEHVFELDPDDPGPHVILYNIYASGGRWNDAARVRKKMKESGVKKE 526

Query: 468 PGCSWIEIRSKVHVFVTGDKNHHQKDEI 494
           P CSW+EI + +H+FV  D+ H Q++EI
Sbjct: 534 PACSWVEIENAIHMFVANDERHPQREEI 526

BLAST of Lsi01G013960 vs. ExPASy Swiss-Prot
Match: Q9SI53 (Pentatricopeptide repeat-containing protein At2g03880, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H44 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.9e-89
Identity = 162/452 (35.84%), Postives = 268/452 (59.29%), Query Frame = 0

Query: 46  SNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSA 105
           ++A QLFD+MP  NV+S T  I   ++   +++AL L   M   N+RPN +T+ +V+ S 
Sbjct: 113 NDAHQLFDQMPQRNVISWTTMISAYSKCKIHQKALELLVLMLRDNVRPNVYTYSSVLRSC 172

Query: 106 TALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYT 165
             + D+   + LH   IK G  S+VFV SAL+D++ KL   E                  
Sbjct: 173 NGMSDV---RMLHCGIIKEGLESDVFVRSALIDVFAKLGEPE------------------ 232

Query: 166 SLISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLP 225
                        DAL VF+EM   + + WN++IGGF+Q   ++ A+ LF  M R GF+ 
Sbjct: 233 -------------DALSVFDEMVTGDAIVWNSIIGGFAQNSRSDVALELFKRMKRAGFIA 292

Query: 226 TQSTFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVF 285
            Q+T    + A   +A + +G   H   VK+    D+ ++N+L+  Y KCGS+ED+L VF
Sbjct: 293 EQATLTSVLRACTGLALLELGMQAHVHIVKY--DQDLILNNALVDMYCKCGSLEDALRVF 352

Query: 286 NKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLV 345
           N+ + ER++++W+ ++ G AQNG  +EA+  +++M  +G KPN +T++ +L+AC+HAGL+
Sbjct: 353 NQ-MKERDVITWSTMISGLAQNGYSQEALKLFERMKSSGTKPNYITIVGVLFACSHAGLL 412

Query: 346 DEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLG 405
           ++G+ YF   K         EHY CM+DLL ++G+L  A + + ++  +P    W+ LLG
Sbjct: 413 EDGWYYFRSMKKLYGIDPVREHYGCMIDLLGKAGKLDDAVKLLNEMECEPDAVTWRTLLG 472

Query: 406 GCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRI 465
            C++  N+ L E+AA++++ALDP D  +Y ++SN ++ + KW S+  +R  M+++G+K+ 
Sbjct: 473 ACRVQRNMVLAEYAAKKVIALDPEDAGTYTLLSNIYANSQKWDSVEEIRTRMRDRGIKKE 527

Query: 466 PGCSWIEIRSKVHVFVTGDKNHHQKDEIYSAL 498
           PGCSWIE+  ++H F+ GD +H Q  E+   L
Sbjct: 533 PGCSWIEVNKQIHAFIIGDNSHPQIVEVSKKL 527

BLAST of Lsi01G013960 vs. ExPASy TrEMBL
Match: A0A6J1GCJ3 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111452968 PE=4 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 3.9e-269
Identity = 457/514 (88.91%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 4   ITIWRKSF-TSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNVVS 63
           I+IWR+S+ +SSC QLIKLKAD +GA  TP + SK APF R E +ARQLFDKMP LNVVS
Sbjct: 2   ISIWRRSYVSSSCRQLIKLKADAYGAVVTPAVQSKKAPFPRSELDARQLFDKMPQLNVVS 61

Query: 64  ATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHICAI 123
           AT+ IGRCARQHQ+EEALCLFSAMFVLNLRPNEFTFGTVIHSATAL DIDIGKQLH CAI
Sbjct: 62  ATSMIGRCARQHQHEEALCLFSAMFVLNLRPNEFTFGTVIHSATALGDIDIGKQLHACAI 121

Query: 124 KTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDALQ 183
           K G HSNVFVGSA LDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLK E+IQDAL+
Sbjct: 122 KIGLHSNVFVGSAALDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKTEKIQDALR 181

Query: 184 VFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANIAS 243
           VF+EMPERN+VSWNAMIGGFSQKGHNEDAVHLFIDML EGFLPTQSTFPCA+CAAANIAS
Sbjct: 182 VFDEMPERNIVSWNAMIGGFSQKGHNEDAVHLFIDMLTEGFLPTQSTFPCALCAAANIAS 241

Query: 244 IGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAVLC 303
           IGIGRSFHACA+KFLG LDVFVSNSLISFYAKCGSMEDSLLVFNKLLD+RN VSWNAVLC
Sbjct: 242 IGIGRSFHACAIKFLGDLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDKRNTVSWNAVLC 301

Query: 304 GYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNPSL 363
           GYAQNGRGKEAIDFYQ+M F+GCKPN VTLLSLLWACNHAGLVDEGYSYFNQAK++NPSL
Sbjct: 302 GYAQNGRGKEAIDFYQRMSFSGCKPNGVTLLSLLWACNHAGLVDEGYSYFNQAKLENPSL 361

Query: 364 LKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAAQR 423
           LKPEHYACMVDLLSRSGQ RRAEEFI DLPFDPGIGFWKALLGGCQIH N+ELGE AAQR
Sbjct: 362 LKPEHYACMVDLLSRSGQFRRAEEFIHDLPFDPGIGFWKALLGGCQIHSNMELGELAAQR 421

Query: 424 ILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 483
           ILALDP DVSSYVMMSNAHSAAGKW S+SILRREMKEKGMKRIPGCSWIEIRSKVHVFVT
Sbjct: 422 ILALDPSDVSSYVMMSNAHSAAGKWHSVSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 481

Query: 484 GDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           GDKNHHQKDEI S L+FFVEH KESEDFNFLS+S
Sbjct: 482 GDKNHHQKDEICSTLKFFVEHLKESEDFNFLSDS 515

BLAST of Lsi01G013960 vs. ExPASy TrEMBL
Match: A0A0A0KX53 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293300 PE=4 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.6e-267
Identity = 452/516 (87.60%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 1   MKPITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNV 60
           MKPITIWRK  TSSC+QL KLK DG+ A GTPPL  +   FS G+ +ARQL+DKMPHLNV
Sbjct: 1   MKPITIWRKPLTSSCTQLTKLKPDGNAAVGTPPLPFQATSFSGGQLDARQLYDKMPHLNV 60

Query: 61  VSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHIC 120
           VSAT  IGRCARQHQ+EEAL LFSAM VLNLRPNEFTFGTVI S  AL DI IGKQLH+C
Sbjct: 61  VSATTAIGRCARQHQHEEALSLFSAMLVLNLRPNEFTFGTVIQSPKALGDIHIGKQLHVC 120

Query: 121 AIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDA 180
           AIKTG HSNVFVGSALLDLYVK+ VIEEAQRAF+DIKMPNVVSYTSLISGYLK+ERI+DA
Sbjct: 121 AIKTGLHSNVFVGSALLDLYVKVGVIEEAQRAFEDIKMPNVVSYTSLISGYLKIERIRDA 180

Query: 181 LQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI 240
           L+VF+EMPERNVVSWN+MIGGFSQKGHNEDAVHLFIDMLREG LPTQSTFPCAICAAANI
Sbjct: 181 LRVFDEMPERNVVSWNSMIGGFSQKGHNEDAVHLFIDMLREGILPTQSTFPCAICAAANI 240

Query: 241 ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV 300
           ASIGIGRSFHACAVKF GKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERN+VSWNAV
Sbjct: 241 ASIGIGRSFHACAVKFFGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNVVSWNAV 300

Query: 301 LCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNP 360
           L G+AQNGRGKEAIDFYQ+MI AGCKPN VT LSLLWACNHAGLVDEGYSYFNQA++DNP
Sbjct: 301 LSGFAQNGRGKEAIDFYQRMILAGCKPNAVTFLSLLWACNHAGLVDEGYSYFNQARLDNP 360

Query: 361 SLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAA 420
           +LLK EHYACMVDLLSRSGQ +RAEEFI DLPFDPGIGFWKALLGGCQIH NVELGE AA
Sbjct: 361 NLLKAEHYACMVDLLSRSGQFKRAEEFIHDLPFDPGIGFWKALLGGCQIHSNVELGELAA 420

Query: 421 QRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVF 480
           QRILALDPGDVSSYVMMSNAHSAAGKW S+SILRREMKEKG+KRIPGCSWIEIRSKVHVF
Sbjct: 421 QRILALDPGDVSSYVMMSNAHSAAGKWHSVSILRREMKEKGLKRIPGCSWIEIRSKVHVF 480

Query: 481 VTGDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           VTGDKNHHQKDEIYSAL+FFVEH KE EDFNFLS+S
Sbjct: 481 VTGDKNHHQKDEIYSALKFFVEHLKEREDFNFLSDS 516

BLAST of Lsi01G013960 vs. ExPASy TrEMBL
Match: A0A6J1IHX7 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111477616 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.1e-266
Identity = 454/514 (88.33%), Postives = 478/514 (93.00%), Query Frame = 0

Query: 4   ITIWRKSF-TSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNVVS 63
           I IWR+S+ +SSC QLIKLKAD HGA  TP + SK  PF RGE +ARQLFDKMP LNVVS
Sbjct: 2   IPIWRRSYVSSSCRQLIKLKADAHGAVVTPAVQSKKDPFPRGELDARQLFDKMPQLNVVS 61

Query: 64  ATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHICAI 123
           ATA IG CARQHQ+EEALCLFSAMFVLNLRPNEFTFGTVIHSATAL DIDIGKQLH CAI
Sbjct: 62  ATAMIGCCARQHQHEEALCLFSAMFVLNLRPNEFTFGTVIHSATALGDIDIGKQLHACAI 121

Query: 124 KTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDALQ 183
           K G HSNVFVGSA LDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLK E+IQ AL+
Sbjct: 122 KIGLHSNVFVGSAALDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKTEKIQAALR 181

Query: 184 VFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANIAS 243
           VF++MPERN+VSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCA+CAAANIAS
Sbjct: 182 VFDKMPERNIVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCALCAAANIAS 241

Query: 244 IGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAVLC 303
           IGIGRSFHACA+KFLG LDVFVSNSLISFYAKCGSMEDSLLVFNKLLD+RN VSWNAVLC
Sbjct: 242 IGIGRSFHACAIKFLGDLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDKRNTVSWNAVLC 301

Query: 304 GYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNPSL 363
           GYAQNGRGKEAIDFYQ+M F+GCKPN VTLLSLLWACNHAGLVDEGYSYFNQAK++NPSL
Sbjct: 302 GYAQNGRGKEAIDFYQRMSFSGCKPNGVTLLSLLWACNHAGLVDEGYSYFNQAKLENPSL 361

Query: 364 LKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAAQR 423
           LKPEHYACMVDLLSRSGQ +RAEEFI DLPFDPGIGFWKALLGGCQIH N+ELGE AAQR
Sbjct: 362 LKPEHYACMVDLLSRSGQFKRAEEFIHDLPFDPGIGFWKALLGGCQIHSNMELGELAAQR 421

Query: 424 ILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 483
           ILALDP DVSSYVMMSNAHSAAGKW S+SILRREMKEKGMKRIPGCSWIE RSKVHVFVT
Sbjct: 422 ILALDPSDVSSYVMMSNAHSAAGKWHSVSILRREMKEKGMKRIPGCSWIESRSKVHVFVT 481

Query: 484 GDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           GDK+HHQKDEI S L+FFVEH KESEDFNFLS+S
Sbjct: 482 GDKSHHQKDEICSTLKFFVEHLKESEDFNFLSDS 515

BLAST of Lsi01G013960 vs. ExPASy TrEMBL
Match: A0A6J1CRE4 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111013558 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.3e-260
Identity = 443/516 (85.85%), Postives = 475/516 (92.05%), Query Frame = 0

Query: 1   MKPITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNV 60
           MKP TIWRKS++SSC QLI    +GHGA  T PL S   PF RG+  A Q+FDKMP LNV
Sbjct: 1   MKPTTIWRKSYSSSCRQLI----NGHGAIETLPLKSSNVPFPRGKLYASQMFDKMPQLNV 60

Query: 61  VSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHIC 120
           VSATA IGR AR++Q+EE LCLFSAMFVLNLRPNEFTFGTVIHSATA+RDIDIGK++H C
Sbjct: 61  VSATAVIGRHARKNQHEEVLCLFSAMFVLNLRPNEFTFGTVIHSATAVRDIDIGKEIHAC 120

Query: 121 AIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDA 180
           A K G HSNVFVGSALLDLY KLSVIEE QRAFDDIK+PNVVSYTSLISGYLKMERIQDA
Sbjct: 121 AKKIGLHSNVFVGSALLDLYAKLSVIEEVQRAFDDIKIPNVVSYTSLISGYLKMERIQDA 180

Query: 181 LQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI 240
           LQVF+EMPERN+VSWNAMIGG SQKGHNEDAVHLFIDMLREGFLPTQSTFP AICAAANI
Sbjct: 181 LQVFDEMPERNIVSWNAMIGGLSQKGHNEDAVHLFIDMLREGFLPTQSTFPSAICAAANI 240

Query: 241 ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV 300
           AS+GIGRSFHACAVKFLGKLDVFV+NSLISFYAKCGSMEDSL++FNKL+ ERNIVSWNAV
Sbjct: 241 ASLGIGRSFHACAVKFLGKLDVFVNNSLISFYAKCGSMEDSLVIFNKLV-ERNIVSWNAV 300

Query: 301 LCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNP 360
           LCGYAQNGRGKEAI FY++M  AG KPN VTLLSLLWACNHAGLVDEGYSYFNQA++DNP
Sbjct: 301 LCGYAQNGRGKEAIGFYERMSSAGVKPNGVTLLSLLWACNHAGLVDEGYSYFNQARLDNP 360

Query: 361 SLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAA 420
           ++LKPEHYAC+VDLLSRSGQ RRAEEFIRDLPFDPGIGFWKALLGGCQIH NVELGE AA
Sbjct: 361 NMLKPEHYACLVDLLSRSGQFRRAEEFIRDLPFDPGIGFWKALLGGCQIHSNVELGELAA 420

Query: 421 QRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVF 480
           QRILALDPGDVSSYVMMSNAHSAAGKWQS+S LRREMKEKGMKR+PGCSWIEIRSKV+VF
Sbjct: 421 QRILALDPGDVSSYVMMSNAHSAAGKWQSVSTLRREMKEKGMKRVPGCSWIEIRSKVNVF 480

Query: 481 VTGDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           VTGD+NHHQKDEIYSA+RFFVEH KESEDFN LS+S
Sbjct: 481 VTGDRNHHQKDEIYSAMRFFVEHLKESEDFNLLSDS 511

BLAST of Lsi01G013960 vs. ExPASy TrEMBL
Match: A0A5A7U174 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001620 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.5e-252
Identity = 424/501 (84.63%), Postives = 456/501 (91.02%), Query Frame = 0

Query: 1   MKPITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNV 60
           MKPI IW K FTSSC QL K+K +G+ A GTPPL SKT PFS G+  ARQLFDKMPHLNV
Sbjct: 1   MKPIRIWIKPFTSSCRQLTKIKPNGNAAVGTPPLGSKTTPFSGGQLAARQLFDKMPHLNV 60

Query: 61  VSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHIC 120
           VSATA IG CARQHQ+EEAL LFSA+ VLNL+PNEFTFGTVIHSA AL DI  GKQLH+C
Sbjct: 61  VSATAAIGHCARQHQHEEALYLFSAILVLNLKPNEFTFGTVIHSAKALGDIQSGKQLHVC 120

Query: 121 AIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDA 180
           AIKTGFHS+VFVGSALLDLY KL  IEEA+RAFDDIKMPNVVSYTSLISGYLK+ERI DA
Sbjct: 121 AIKTGFHSDVFVGSALLDLYFKLGFIEEAERAFDDIKMPNVVSYTSLISGYLKIERIHDA 180

Query: 181 LQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI 240
           L+VF+EMPERNVVSWN+MI GFSQKGHNEDAVHLF+DMLREG LPTQSTF CAICAAANI
Sbjct: 181 LRVFDEMPERNVVSWNSMISGFSQKGHNEDAVHLFVDMLREGILPTQSTFTCAICAAANI 240

Query: 241 ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV 300
           ASIGIGRSFHACAVKF GKLDVFVSNSLISFYAKCGSMEDSLLVFNKL +ERN+VSWNA+
Sbjct: 241 ASIGIGRSFHACAVKFFGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLRNERNVVSWNAI 300

Query: 301 LCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNP 360
           L G+AQNGRGKEAIDFYQ+MI AGCKPNDVT LSLLWACNHAGLVDEGYSYFNQA++ NP
Sbjct: 301 LGGFAQNGRGKEAIDFYQRMILAGCKPNDVTFLSLLWACNHAGLVDEGYSYFNQARLINP 360

Query: 361 SLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAA 420
           +LLK EHYACMVDLLSRSGQ +RAEEFI DLPF+PGIGFWKALLGGCQIH NVELGE AA
Sbjct: 361 NLLKAEHYACMVDLLSRSGQFKRAEEFIHDLPFNPGIGFWKALLGGCQIHSNVELGELAA 420

Query: 421 QRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVF 480
           QRILALDPGD SSYVMMSNAHSAAGKWQ++S+ RREMKEKG+KRIPGCSWIEIRSK+HVF
Sbjct: 421 QRILALDPGDFSSYVMMSNAHSAAGKWQNVSLARREMKEKGLKRIPGCSWIEIRSKIHVF 480

Query: 481 VTGDKNHHQKDEIYSALRFFV 502
           V GDKNHHQKDEIYS L+  +
Sbjct: 481 VNGDKNHHQKDEIYSTLKLLL 501

BLAST of Lsi01G013960 vs. NCBI nr
Match: XP_038882815.1 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 956.1 bits (2470), Expect = 1.3e-274
Identity = 459/516 (88.95%), Postives = 488/516 (94.57%), Query Frame = 0

Query: 1   MKPITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNV 60
           MKPI++WRKSFTSSC + IKLK DGHGA GTPPL S  APF R E NA QLFDK PHLNV
Sbjct: 1   MKPISMWRKSFTSSCRKSIKLKPDGHGAVGTPPLQSNAAPFPRSELNAHQLFDKTPHLNV 60

Query: 61  VSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHIC 120
           +SAT EIGRCARQHQ+EEAL LFSAM +LNLRPNEFTFGTVIHSATAL DIDIGKQLH+C
Sbjct: 61  ISATVEIGRCARQHQHEEALYLFSAMLMLNLRPNEFTFGTVIHSATALGDIDIGKQLHVC 120

Query: 121 AIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDA 180
           A+KTG HSNVFVGSALL LYVKLSVIEEAQ+AFDDIKMPNVVSYTSLISGYLKMERI DA
Sbjct: 121 ALKTGLHSNVFVGSALLHLYVKLSVIEEAQKAFDDIKMPNVVSYTSLISGYLKMERIHDA 180

Query: 181 LQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI 240
           L+VF++MPERN+VSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI
Sbjct: 181 LRVFDKMPERNIVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI 240

Query: 241 ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV 300
           ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV
Sbjct: 241 ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV 300

Query: 301 LCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNP 360
           LCGYAQNGRGKEAIDFYQ+MIFAGCKPN VTLLSLLWACNHAGLVDEGY+YFNQA+++NP
Sbjct: 301 LCGYAQNGRGKEAIDFYQRMIFAGCKPNGVTLLSLLWACNHAGLVDEGYAYFNQARLNNP 360

Query: 361 SLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAA 420
           +LLKPEHYACMVDLLSRSGQ RRA+EFI DLPFDPGIGFWKALLGGCQIH NVELGE AA
Sbjct: 361 NLLKPEHYACMVDLLSRSGQFRRAKEFIHDLPFDPGIGFWKALLGGCQIHSNVELGELAA 420

Query: 421 QRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVF 480
           Q+ILALDPGDVSSYVMMSNAHSAAGKWQ++SILRR+MKEKG+KRIPGCSWI++RSKVH+F
Sbjct: 421 QKILALDPGDVSSYVMMSNAHSAAGKWQNVSILRRKMKEKGLKRIPGCSWIDVRSKVHIF 480

Query: 481 VTGDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           VT DKNH QKDEIY ALRFFVEH KESEDFNFLS+S
Sbjct: 481 VTSDKNHRQKDEIYVALRFFVEHLKESEDFNFLSDS 516

BLAST of Lsi01G013960 vs. NCBI nr
Match: KAG6603678.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 939.5 bits (2427), Expect = 1.2e-269
Identity = 458/514 (89.11%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 4   ITIWRKSF-TSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNVVS 63
           I IWR+S+ +SSC QL+KLKAD HGA  TP   SK APF RGE +ARQLFDKMP LNVVS
Sbjct: 2   IPIWRRSYVSSSCRQLMKLKADAHGAVVTPAEQSKKAPFPRGELDARQLFDKMPQLNVVS 61

Query: 64  ATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHICAI 123
           ATA IGRCARQHQ+EEALCLFSAMFVLNLRPNEFTFGTVIHSATAL DID+GKQLH CAI
Sbjct: 62  ATAMIGRCARQHQHEEALCLFSAMFVLNLRPNEFTFGTVIHSATALGDIDVGKQLHACAI 121

Query: 124 KTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDALQ 183
           K G HSNVFVGSA LDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLK E+IQDAL+
Sbjct: 122 KIGLHSNVFVGSAALDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKTEKIQDALR 181

Query: 184 VFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANIAS 243
           VF+EMPERN+VSWNA+IGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCA+CAAANIAS
Sbjct: 182 VFDEMPERNIVSWNAVIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCALCAAANIAS 241

Query: 244 IGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAVLC 303
           IGIGRSFHACA+KFLG LDVFVSNSLISFYAKCGSMEDSLLVFNKLLD+RN VSWNAVLC
Sbjct: 242 IGIGRSFHACAIKFLGDLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDKRNTVSWNAVLC 301

Query: 304 GYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNPSL 363
           GYAQNGRGKEAIDFYQ+M F+GCKPN VTLLSLLWACNHAGLVDEGYSYFNQAK++NPSL
Sbjct: 302 GYAQNGRGKEAIDFYQRMSFSGCKPNGVTLLSLLWACNHAGLVDEGYSYFNQAKLENPSL 361

Query: 364 LKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAAQR 423
           LKPEHYACMVDLLSRSGQ RRAEEFI DLPFDPGIGFWKALLGGCQIH N+ELGE AAQR
Sbjct: 362 LKPEHYACMVDLLSRSGQFRRAEEFIHDLPFDPGIGFWKALLGGCQIHSNMELGELAAQR 421

Query: 424 ILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 483
           ILALDP DVSSYVMMSNAHSAAGKW S+SILRREMKEKGMKRIPGCSWIEIRSKVHVFVT
Sbjct: 422 ILALDPSDVSSYVMMSNAHSAAGKWHSVSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 481

Query: 484 GDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           GDKNHHQKDEI S L+FFVEH KESEDFNFLS+S
Sbjct: 482 GDKNHHQKDEICSTLKFFVEHLKESEDFNFLSDS 515

BLAST of Lsi01G013960 vs. NCBI nr
Match: XP_022949636.1 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 936.8 bits (2420), Expect = 8.0e-269
Identity = 457/514 (88.91%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 4   ITIWRKSF-TSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNVVS 63
           I+IWR+S+ +SSC QLIKLKAD +GA  TP + SK APF R E +ARQLFDKMP LNVVS
Sbjct: 2   ISIWRRSYVSSSCRQLIKLKADAYGAVVTPAVQSKKAPFPRSELDARQLFDKMPQLNVVS 61

Query: 64  ATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHICAI 123
           AT+ IGRCARQHQ+EEALCLFSAMFVLNLRPNEFTFGTVIHSATAL DIDIGKQLH CAI
Sbjct: 62  ATSMIGRCARQHQHEEALCLFSAMFVLNLRPNEFTFGTVIHSATALGDIDIGKQLHACAI 121

Query: 124 KTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDALQ 183
           K G HSNVFVGSA LDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLK E+IQDAL+
Sbjct: 122 KIGLHSNVFVGSAALDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKTEKIQDALR 181

Query: 184 VFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANIAS 243
           VF+EMPERN+VSWNAMIGGFSQKGHNEDAVHLFIDML EGFLPTQSTFPCA+CAAANIAS
Sbjct: 182 VFDEMPERNIVSWNAMIGGFSQKGHNEDAVHLFIDMLTEGFLPTQSTFPCALCAAANIAS 241

Query: 244 IGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAVLC 303
           IGIGRSFHACA+KFLG LDVFVSNSLISFYAKCGSMEDSLLVFNKLLD+RN VSWNAVLC
Sbjct: 242 IGIGRSFHACAIKFLGDLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDKRNTVSWNAVLC 301

Query: 304 GYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNPSL 363
           GYAQNGRGKEAIDFYQ+M F+GCKPN VTLLSLLWACNHAGLVDEGYSYFNQAK++NPSL
Sbjct: 302 GYAQNGRGKEAIDFYQRMSFSGCKPNGVTLLSLLWACNHAGLVDEGYSYFNQAKLENPSL 361

Query: 364 LKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAAQR 423
           LKPEHYACMVDLLSRSGQ RRAEEFI DLPFDPGIGFWKALLGGCQIH N+ELGE AAQR
Sbjct: 362 LKPEHYACMVDLLSRSGQFRRAEEFIHDLPFDPGIGFWKALLGGCQIHSNMELGELAAQR 421

Query: 424 ILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 483
           ILALDP DVSSYVMMSNAHSAAGKW S+SILRREMKEKGMKRIPGCSWIEIRSKVHVFVT
Sbjct: 422 ILALDPSDVSSYVMMSNAHSAAGKWHSVSILRREMKEKGMKRIPGCSWIEIRSKVHVFVT 481

Query: 484 GDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           GDKNHHQKDEI S L+FFVEH KESEDFNFLS+S
Sbjct: 482 GDKNHHQKDEICSTLKFFVEHLKESEDFNFLSDS 515

BLAST of Lsi01G013960 vs. NCBI nr
Match: XP_023544385.1 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.4 bits (2419), Expect = 1.0e-268
Identity = 455/513 (88.69%), Postives = 477/513 (92.98%), Query Frame = 0

Query: 4   ITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNVVSA 63
           I IWR+S+ SSC QLIKLKAD HGA  TP   SK  PF RGE +ARQLFDKMP +NVVSA
Sbjct: 2   IPIWRRSYASSCRQLIKLKADAHGAVVTPAEQSKKPPFPRGELDARQLFDKMPQINVVSA 61

Query: 64  TAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHICAIK 123
           TA IGRCARQHQ+EEAL LFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLH CAIK
Sbjct: 62  TAMIGRCARQHQHEEALGLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHACAIK 121

Query: 124 TGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDALQV 183
            G HSNVFVGSA LDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLK E+IQDA +V
Sbjct: 122 IGLHSNVFVGSAALDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKTEKIQDAFRV 181

Query: 184 FEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANIASI 243
           F+EMPERN+VSWNAMIGGFS+KGHNEDAVHLFIDMLREGFLPTQSTFPCA+CAAANIASI
Sbjct: 182 FDEMPERNIVSWNAMIGGFSRKGHNEDAVHLFIDMLREGFLPTQSTFPCALCAAANIASI 241

Query: 244 GIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAVLCG 303
           GIGRSFHAC +KFLG LDVFVSNSLISFYAKCGSMEDSLLVFNKLLD+RN VSWNAVLCG
Sbjct: 242 GIGRSFHACGIKFLGDLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDKRNTVSWNAVLCG 301

Query: 304 YAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNPSLL 363
           YAQNGRGKEAIDFYQ+M F+GCKPN VTLLSLLWACNHAGLVDEGYSYFNQAK++NPSLL
Sbjct: 302 YAQNGRGKEAIDFYQRMSFSGCKPNGVTLLSLLWACNHAGLVDEGYSYFNQAKLENPSLL 361

Query: 364 KPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAAQRI 423
           KPEHYACMVDLLSRSGQ R+AEEFIRDLPFDPGIGFWKALLGGCQIH N+ELGE AAQRI
Sbjct: 362 KPEHYACMVDLLSRSGQFRQAEEFIRDLPFDPGIGFWKALLGGCQIHSNMELGELAAQRI 421

Query: 424 LALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVFVTG 483
           LALDP DVSSYVMMSN HSAAGKW S+SILRREMKEKGMKRIPGCSWIEIRSKVHVFVTG
Sbjct: 422 LALDPSDVSSYVMMSNVHSAAGKWHSVSILRREMKEKGMKRIPGCSWIEIRSKVHVFVTG 481

Query: 484 DKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           DKNHHQKDEI S L+FFVEH KESEDFNFLS+S
Sbjct: 482 DKNHHQKDEICSTLKFFVEHLKESEDFNFLSDS 514

BLAST of Lsi01G013960 vs. NCBI nr
Match: XP_004142091.1 (pentatricopeptide repeat-containing protein At5g42450, mitochondrial [Cucumis sativus] >KGN54220.1 hypothetical protein Csa_017944 [Cucumis sativus])

HSP 1 Score: 931.4 bits (2406), Expect = 3.4e-267
Identity = 452/516 (87.60%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 1   MKPITIWRKSFTSSCSQLIKLKADGHGADGTPPLLSKTAPFSRGESNARQLFDKMPHLNV 60
           MKPITIWRK  TSSC+QL KLK DG+ A GTPPL  +   FS G+ +ARQL+DKMPHLNV
Sbjct: 1   MKPITIWRKPLTSSCTQLTKLKPDGNAAVGTPPLPFQATSFSGGQLDARQLYDKMPHLNV 60

Query: 61  VSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATALRDIDIGKQLHIC 120
           VSAT  IGRCARQHQ+EEAL LFSAM VLNLRPNEFTFGTVI S  AL DI IGKQLH+C
Sbjct: 61  VSATTAIGRCARQHQHEEALSLFSAMLVLNLRPNEFTFGTVIQSPKALGDIHIGKQLHVC 120

Query: 121 AIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSLISGYLKMERIQDA 180
           AIKTG HSNVFVGSALLDLYVK+ VIEEAQRAF+DIKMPNVVSYTSLISGYLK+ERI+DA
Sbjct: 121 AIKTGLHSNVFVGSALLDLYVKVGVIEEAQRAFEDIKMPNVVSYTSLISGYLKIERIRDA 180

Query: 181 LQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQSTFPCAICAAANI 240
           L+VF+EMPERNVVSWN+MIGGFSQKGHNEDAVHLFIDMLREG LPTQSTFPCAICAAANI
Sbjct: 181 LRVFDEMPERNVVSWNSMIGGFSQKGHNEDAVHLFIDMLREGILPTQSTFPCAICAAANI 240

Query: 241 ASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNIVSWNAV 300
           ASIGIGRSFHACAVKF GKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERN+VSWNAV
Sbjct: 241 ASIGIGRSFHACAVKFFGKLDVFVSNSLISFYAKCGSMEDSLLVFNKLLDERNVVSWNAV 300

Query: 301 LCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDEGYSYFNQAKIDNP 360
           L G+AQNGRGKEAIDFYQ+MI AGCKPN VT LSLLWACNHAGLVDEGYSYFNQA++DNP
Sbjct: 301 LSGFAQNGRGKEAIDFYQRMILAGCKPNAVTFLSLLWACNHAGLVDEGYSYFNQARLDNP 360

Query: 361 SLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLGGCQIHLNVELGEFAA 420
           +LLK EHYACMVDLLSRSGQ +RAEEFI DLPFDPGIGFWKALLGGCQIH NVELGE AA
Sbjct: 361 NLLKAEHYACMVDLLSRSGQFKRAEEFIHDLPFDPGIGFWKALLGGCQIHSNVELGELAA 420

Query: 421 QRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRIPGCSWIEIRSKVHVF 480
           QRILALDPGDVSSYVMMSNAHSAAGKW S+SILRREMKEKG+KRIPGCSWIEIRSKVHVF
Sbjct: 421 QRILALDPGDVSSYVMMSNAHSAAGKWHSVSILRREMKEKGLKRIPGCSWIEIRSKVHVF 480

Query: 481 VTGDKNHHQKDEIYSALRFFVEHQKESEDFNFLSNS 517
           VTGDKNHHQKDEIYSAL+FFVEH KE EDFNFLS+S
Sbjct: 481 VTGDKNHHQKDEIYSALKFFVEHLKEREDFNFLSDS 516

BLAST of Lsi01G013960 vs. TAIR 10
Match: AT5G42450.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 584.7 bits (1506), Expect = 7.2e-167
Identity = 282/468 (60.26%), Postives = 364/468 (77.78%), Query Frame = 0

Query: 47  NARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSAT 106
           NA ++FD++P L+V+SATA IGR  ++ ++ EA   F  +  L +RPNEFTFGTVI S+T
Sbjct: 45  NAHKVFDEIPELDVISATAVIGRFVKESRHVEASQAFKRLLCLGIRPNEFTFGTVIGSST 104

Query: 107 ALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTS 166
             RD+ +GKQLH  A+K G  SNVFVGSA+L+ YVKLS + +A+R FDD + PNVVS T+
Sbjct: 105 TSRDVKLGKQLHCYALKMGLASNVFVGSAVLNCYVKLSTLTDARRCFDDTRDPNVVSITN 164

Query: 167 LISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREG-FLP 226
           LISGYLK    ++AL +F  MPER+VV+WNA+IGGFSQ G NE+AV+ F+DMLREG  +P
Sbjct: 165 LISGYLKKHEFEEALSLFRAMPERSVVTWNAVIGGFSQTGRNEEAVNTFVDMLREGVVIP 224

Query: 227 TQSTFPCAICAAANIASIGIGRSFHACAVKFLGK-LDVFVSNSLISFYAKCGSMEDSLLV 286
            +STFPCAI A +NIAS G G+S HACA+KFLGK  +VFV NSLISFY+KCG+MEDSLL 
Sbjct: 225 NESTFPCAITAISNIASHGAGKSIHACAIKFLGKRFNVFVWNSLISFYSKCGNMEDSLLA 284

Query: 287 FNKLLDE-RNIVSWNAVLCGYAQNGRGKEAIDFYQKMI-FAGCKPNDVTLLSLLWACNHA 346
           FNKL +E RNIVSWN+++ GYA NGRG+EA+  ++KM+     +PN+VT+L +L+ACNHA
Sbjct: 285 FNKLEEEQRNIVSWNSMIWGYAHNGRGEEAVAMFEKMVKDTNLRPNNVTILGVLFACNHA 344

Query: 347 GLVDEGYSYFNQA--KIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFW 406
           GL+ EGY YFN+A    D+P+LL+ EHYACMVD+LSRSG+ + AEE I+ +P DPGIGFW
Sbjct: 345 GLIQEGYMYFNKAVNDYDDPNLLELEHYACMVDMLSRSGRFKEAEELIKSMPLDPGIGFW 404

Query: 407 KALLGGCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEK 466
           KALLGGCQIH N  L + AA +IL LDP DVSSYVM+SNA+SA   WQ++S++RR+MKE 
Sbjct: 405 KALLGGCQIHSNKRLAKLAASKILELDPRDVSSYVMLSNAYSAMENWQNVSLIRRKMKET 464

Query: 467 GMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESE 509
           G+KR  GCSWIE+R ++ VFV  DKN+  KDE+Y  L    +H +E+E
Sbjct: 465 GLKRFTGCSWIEVRDQIRVFVNADKNNELKDEVYRMLALVSQHLEENE 512

BLAST of Lsi01G013960 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 9.9e-100
Identity = 177/471 (37.58%), Postives = 289/471 (61.36%), Query Frame = 0

Query: 46  SNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSA 105
           ++A+++FD+M   NVVS  + I    +     EAL +F  M    + P+E T  +VI + 
Sbjct: 204 NDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISAC 263

Query: 106 TALRDIDIGKQLHICAIKTG-FHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSY 165
            +L  I +G+++H   +K     +++ + +A +D+Y K S I+EA+  FD + + NV++ 
Sbjct: 264 ASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAE 323

Query: 166 TSLISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFL 225
           TS+ISGY      + A  +F +M ERNVVSWNA+I G++Q G NE+A+ LF  + RE   
Sbjct: 324 TSMISGYAMAASTKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVC 383

Query: 226 PTQSTFPCAICAAANIASIGIGRSFHACAVKFLGKL------DVFVSNSLISFYAKCGSM 285
           PT  +F   + A A++A + +G   H   +K   K       D+FV NSLI  Y KCG +
Sbjct: 384 PTHYSFANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCV 443

Query: 286 EDSLLVFNKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWA 345
           E+  LVF K++ ER+ VSWNA++ G+AQNG G EA++ +++M+ +G KP+ +T++ +L A
Sbjct: 444 EEGYLVFRKMM-ERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSA 503

Query: 346 CNHAGLVDEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIG 405
           C HAG V+EG  YF+    D       +HY CMVDLL R+G L  A+  I ++P  P   
Sbjct: 504 CGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSV 563

Query: 406 FWKALLGGCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMK 465
            W +LL  C++H N+ LG++ A+++L ++P +   YV++SN ++  GKW+ +  +R+ M+
Sbjct: 564 IWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMR 623

Query: 466 EKGMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESED 510
           ++G+ + PGCSWI+I+   HVF+  DK+H +K +I+S L   +   +  +D
Sbjct: 624 KEGVTKQPGCSWIKIQGHDHVFMVKDKSHPRKKQIHSLLDILIAEMRPEQD 673

BLAST of Lsi01G013960 vs. TAIR 10
Match: AT3G02330.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.8e-94
Identity = 176/473 (37.21%), Postives = 279/473 (58.99%), Query Frame = 0

Query: 46  SNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSA 105
           + A ++FD+M   + VS  A I    +  +  E L LF +M    + P+EFTFG+++ + 
Sbjct: 434 AEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSRIEPDEFTFGSILKAC 493

Query: 106 TALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYT 165
           T    +  G ++H   +K+G  SN  VG +L+D+Y K  +IEEA++              
Sbjct: 494 TG-GSLGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEK-------------- 553

Query: 166 SLISGYLKMERIQDALQVFEEMPERNV----VSWNAMIGGFSQKGHNEDAVHLFIDMLRE 225
            + S + +   +   ++  E+M  + +    VSWN++I G+  K  +EDA  LF  M+  
Sbjct: 554 -IHSRFFQRANVSGTMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRMMEM 613

Query: 226 GFLPTQSTFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDS 285
           G  P + T+   +   AN+AS G+G+  HA  +K   + DV++ ++L+  Y+KCG + DS
Sbjct: 614 GITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDLHDS 673

Query: 286 LLVFNKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNH 345
            L+F K L  R+ V+WNA++CGYA +G+G+EAI  +++MI    KPN VT +S+L AC H
Sbjct: 674 RLMFEKSL-RRDFVTWNAMICGYAHHGKGEEAIQLFERMILENIKPNHVTFISILRACAH 733

Query: 346 AGLVDEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWK 405
            GL+D+G  YF   K D     +  HY+ MVD+L +SG+++RA E IR++PF+     W+
Sbjct: 734 MGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADDVIWR 793

Query: 406 ALLGGCQIHL-NVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEK 465
            LLG C IH  NVE+ E A   +L LDP D S+Y ++SN ++ AG W+ +S LRR M+  
Sbjct: 794 TLLGVCTIHRNNVEVAEEATAALLRLDPQDSSAYTLLSNVYADAGMWEKVSDLRRNMRGF 853

Query: 466 GMKRIPGCSWIEIRSKVHVFVTGDKNHHQKDEIYSALRFFVEHQKESEDFNFL 514
            +K+ PGCSW+E++ ++HVF+ GDK H + +EIY  L       K  +D +F+
Sbjct: 854 KLKKEPGCSWVELKDELHVFLVGDKAHPRWEEIYEELGLIYSEMKPFDDSSFV 889

BLAST of Lsi01G013960 vs. TAIR 10
Match: AT3G24000.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 339.3 bits (869), Expect = 5.2e-93
Identity = 173/448 (38.62%), Postives = 266/448 (59.38%), Query Frame = 0

Query: 48  ARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSATA 107
           AR++F+KMP  + V+ T  I   ++  +  +AL  F+ M      PNEFT  +VI +A A
Sbjct: 114 ARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAA 173

Query: 108 LRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYTSL 167
            R    G QLH   +K GF SNV VGSALLDLY +  ++++AQ  FD ++          
Sbjct: 174 ERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALE---------- 233

Query: 168 ISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLPTQ 227
                                 RN VSWNA+I G +++   E A+ LF  MLR+GF P+ 
Sbjct: 234 ---------------------SRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSH 293

Query: 228 STFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVFNK 287
            ++     A ++   +  G+  HA  +K   KL  F  N+L+  YAK GS+ D+  +F++
Sbjct: 294 FSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDR 353

Query: 288 LLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLVDE 347
           L  +R++VSWN++L  YAQ+G GKEA+ ++++M   G +PN+++ LS+L AC+H+GL+DE
Sbjct: 354 LA-KRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSHSGLLDE 413

Query: 348 GYSYFNQAKIDNPSLLKPE--HYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLG 407
           G+ Y+   K D    + PE  HY  +VDLL R+G L RA  FI ++P +P    WKALL 
Sbjct: 414 GWHYYELMKKDG---IVPEAWHYVTVVDLLGRAGDLNRALRFIEEMPIEPTAAIWKALLN 473

Query: 408 GCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRI 467
            C++H N ELG +AA+ +  LDP D   +V++ N +++ G+W   + +R++MKE G+K+ 
Sbjct: 474 ACRMHKNTELGAYAAEHVFELDPDDPGPHVILYNIYASGGRWNDAARVRKKMKESGVKKE 526

Query: 468 PGCSWIEIRSKVHVFVTGDKNHHQKDEI 494
           P CSW+EI + +H+FV  D+ H Q++EI
Sbjct: 534 PACSWVEIENAIHMFVANDERHPQREEI 526

BLAST of Lsi01G013960 vs. TAIR 10
Match: AT2G03880.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 4.2e-90
Identity = 162/452 (35.84%), Postives = 268/452 (59.29%), Query Frame = 0

Query: 46  SNARQLFDKMPHLNVVSATAEIGRCARQHQYEEALCLFSAMFVLNLRPNEFTFGTVIHSA 105
           ++A QLFD+MP  NV+S T  I   ++   +++AL L   M   N+RPN +T+ +V+ S 
Sbjct: 113 NDAHQLFDQMPQRNVISWTTMISAYSKCKIHQKALELLVLMLRDNVRPNVYTYSSVLRSC 172

Query: 106 TALRDIDIGKQLHICAIKTGFHSNVFVGSALLDLYVKLSVIEEAQRAFDDIKMPNVVSYT 165
             + D+   + LH   IK G  S+VFV SAL+D++ KL   E                  
Sbjct: 173 NGMSDV---RMLHCGIIKEGLESDVFVRSALIDVFAKLGEPE------------------ 232

Query: 166 SLISGYLKMERIQDALQVFEEMPERNVVSWNAMIGGFSQKGHNEDAVHLFIDMLREGFLP 225
                        DAL VF+EM   + + WN++IGGF+Q   ++ A+ LF  M R GF+ 
Sbjct: 233 -------------DALSVFDEMVTGDAIVWNSIIGGFAQNSRSDVALELFKRMKRAGFIA 292

Query: 226 TQSTFPCAICAAANIASIGIGRSFHACAVKFLGKLDVFVSNSLISFYAKCGSMEDSLLVF 285
            Q+T    + A   +A + +G   H   VK+    D+ ++N+L+  Y KCGS+ED+L VF
Sbjct: 293 EQATLTSVLRACTGLALLELGMQAHVHIVKY--DQDLILNNALVDMYCKCGSLEDALRVF 352

Query: 286 NKLLDERNIVSWNAVLCGYAQNGRGKEAIDFYQKMIFAGCKPNDVTLLSLLWACNHAGLV 345
           N+ + ER++++W+ ++ G AQNG  +EA+  +++M  +G KPN +T++ +L+AC+HAGL+
Sbjct: 353 NQ-MKERDVITWSTMISGLAQNGYSQEALKLFERMKSSGTKPNYITIVGVLFACSHAGLL 412

Query: 346 DEGYSYFNQAKIDNPSLLKPEHYACMVDLLSRSGQLRRAEEFIRDLPFDPGIGFWKALLG 405
           ++G+ YF   K         EHY CM+DLL ++G+L  A + + ++  +P    W+ LLG
Sbjct: 413 EDGWYYFRSMKKLYGIDPVREHYGCMIDLLGKAGKLDDAVKLLNEMECEPDAVTWRTLLG 472

Query: 406 GCQIHLNVELGEFAAQRILALDPGDVSSYVMMSNAHSAAGKWQSMSILRREMKEKGMKRI 465
            C++  N+ L E+AA++++ALDP D  +Y ++SN ++ + KW S+  +R  M+++G+K+ 
Sbjct: 473 ACRVQRNMVLAEYAAKKVIALDPEDAGTYTLLSNIYANSQKWDSVEEIRTRMRDRGIKKE 527

Query: 466 PGCSWIEIRSKVHVFVTGDKNHHQKDEIYSAL 498
           PGCSWIE+  ++H F+ GD +H Q  E+   L
Sbjct: 533 PGCSWIEVNKQIHAFIIGDNSHPQIVEVSKKL 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIH21.0e-16560.26Pentatricopeptide repeat-containing protein At5g42450, mitochondrial OS=Arabidop... [more]
Q9SIT71.4e-9837.58Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
Q9FWA63.9e-9337.21Pentatricopeptide repeat-containing protein At3g02330, mitochondrial OS=Arabidop... [more]
Q9LIQ77.4e-9238.62Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidop... [more]
Q9SI535.9e-8935.84Pentatricopeptide repeat-containing protein At2g03880, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1GCJ33.9e-26988.91pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like OS=Cuc... [more]
A0A0A0KX531.6e-26787.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293300 PE=4 SV=1[more]
A0A6J1IHX71.1e-26688.33pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like OS=Cuc... [more]
A0A6J1CRE47.3e-26085.85pentatricopeptide repeat-containing protein At5g42450, mitochondrial OS=Momordic... [more]
A0A5A7U1741.5e-25284.63Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
XP_038882815.11.3e-27488.95pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like [Benin... [more]
KAG6603678.11.2e-26989.11Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022949636.18.0e-26988.91pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like [Cucur... [more]
XP_023544385.11.0e-26888.69pentatricopeptide repeat-containing protein At5g42450, mitochondrial-like [Cucur... [more]
XP_004142091.13.4e-26787.60pentatricopeptide repeat-containing protein At5g42450, mitochondrial [Cucumis sa... [more]
Match NameE-valueIdentityDescription
AT5G42450.17.2e-16760.26Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G13600.19.9e-10037.58Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G02330.12.8e-9437.21Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G24000.15.2e-9338.62Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G03880.14.2e-9035.84Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 266..290
e-value: 0.018
score: 15.2
coord: 433..462
e-value: 1.1
score: 9.7
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 295..328
e-value: 1.3E-9
score: 35.6
coord: 193..226
e-value: 3.1E-5
score: 21.8
coord: 162..193
e-value: 1.6E-7
score: 29.0
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 190..234
e-value: 5.9E-11
score: 42.4
coord: 292..338
e-value: 4.0E-11
score: 42.9
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 158..187
e-value: 1.6E-7
score: 30.9
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 430..464
score: 8.571795
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 160..190
score: 10.972319
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 191..225
score: 12.265752
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 293..327
score: 12.353442
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 249..363
e-value: 8.4E-23
score: 83.3
coord: 145..248
e-value: 2.7E-22
score: 81.6
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 43..144
e-value: 1.3E-11
score: 46.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 364..499
e-value: 6.1E-6
score: 27.8
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 267..449
NoneNo IPR availablePANTHERPTHR47926:SF288OS01G0839400 PROTEINcoord: 46..506
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 46..506

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G013960.1Lsi01G013960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding