Lsi01G012920 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G012920
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAquaporin TIP1-1
Locationchr01: 11208708 .. 11210685 (+)
RNA-Seq ExpressionLsi01G012920
SyntenyLsi01G012920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCTAAAAATTTCTCCAATTATTACCGGCTACCTGTTGTAGCCGGTTAAGCCTGCCCTTGAACGTTCCATTATAAGGGCTAGAAATGGACGTGAAAGTGGAGTACTACTACTACTACAAGTGAAAAAAAGAGAGTTAGAATTAATTTTATAGCTCTCTAATTATAGTCATGCCGATCCGTAACATTGCAATTGGAAGGCCCGAAGAGGCTACTCATCCAGATGCTTTGAAGGCTGGGTTGGCTGAGTTCATTTCCACGTTGATTTTTGTCTTTGCTGGCCAAGGTTCCGGCATCGCCTTCAGTAAGCTTACCGAAGGCGGCGCCACCACTCCTGCCGGTCTCATCTCCGCATCCATCGCCCATGCCTTTGCCCTTTTTGTTGCCGTCTCCGTCGGTGCCAACATCTCTGGTGGCCATGTCAACCCCGCCGTTACCTTCGGTGCCTTCGTTGGCGGTAACATCACCCTCTTACGTGGCATCATCTACTGGATTGCCCAACTCCTCGGATCCGTCGTCGCATGCTTGCTCCTTAAATTCGTCACCGGCTTGGTGAGTTTTACCTAAATTTGCTAATGTCTTACCACAGTTAAACTATGCAAAATAGATAACAAAACTAATAGGATAAAAAAATTTTAGTCTGAACATAGCTAAACTGATTTAATAAATAATAAAAAGAAAATTATAGGTTGAAATCTTCACTATCGCTCATTCTTGACTTATTTTGAAAAAGAAATTGTGACAAAAAAAAGGGACAAAACTATGTAATTTTTTTTAATCTAGAAAATAAATAAAAAATATCGTTTAAAATTATATCACTCACAAAGTAAATAGAAGAAGATCAAATTATTGGATGTTGCAATGAGATATTATTGAATGATGCTATCACAATGTGACAAATCTACACCGGAGAAAAATTATTTAAGGCCCCACCATACTATAGCGGGATCTCAAATTTATATTTAATAATTATTAAAGCATATGTAGAATTTTAAATATTAACTTGAAAATTATTATGAGACAGCTACATTGTTATTAAACAATATTTCTTTTAAGTATATGGTTAGTTAGAGTTTAAATATTGGAGTTAGATGTCGTTATTTCCGTTACTAGGGCAGAAAATCACCTTTTTTTTATTTTTTAATTAAGTTATTAACCGATGTTGAGGGTTTTGTTCCTGTGCATGGCAGCCCACCGGAAGCTTCGCTCTATCAGCTGGAGTTGGTGAAATAAACGCCTTCGTTTTCGAGATCGTGATGACATTCGGATTGGTGTACACAGTGTACGCGACCGCAGTGGATCCCAAGAAGGGCAGTTTGGGAACTATTGCACCCATCGCAATCGGTTTCATCGTCGGAGCTAACATTTTGGCCGGAGGAGCATTCACCGGAGCATCCATGAACCCAGCCGTGGCCTTCGGTCCATCAGTAGTGAGCTGGTCATGGAGCAGCCACTGGGTCTACTGGGCCGGGCCTCTGATCGGAGGTGGGCTTGCTGGACTCATCTACGAGTTCATCTTCATTTCCAGCACCCACGAGCAGCTCCCAACCACCGACTACTAAGTCAATCACCACAACCCACGGCGGCGGCGACGGAGGCCACTTTTTTTCTCTCCTTAATTTGGTGTATTTGGTTTTGTTAATTTTCTTTTTATCGGACGGGTTTGATCGTGTCTCATTTGTGACCGTTGATCTTTTCATCATCTTTAATTTGTGGTCGTCGTCGTGTGCAGTGCCTGGACCTACGATGATTTGATTGAAGATGAAAATCAGGGCCAGGTTGTAATTTATGTTATTCTGCTCCTGTCTGTACGTGAATTATTTTCAAGTCTTTTTTCTTCTTTGTCCATTTCGATAATTTTCTAAATTTAAGATTTTCTTTTCCTCCTTTCCATTTTCTGAATAATGCAGTGGGACCCATTTTCCCCATCCCGGAATAACTTTATAATAATAATCCCAACCTTTTATATTTTTCACAAA

mRNA sequence

TTTCCTAAAAATTTCTCCAATTATTACCGGCTACCTGTTGTAGCCGGTTAAGCCTGCCCTTGAACGTTCCATTATAAGGGCTAGAAATGGACGTGAAAGTGGAGTACTACTACTACTACAAGTGAAAAAAAGAGAGTTAGAATTAATTTTATAGCTCTCTAATTATAGTCATGCCGATCCGTAACATTGCAATTGGAAGGCCCGAAGAGGCTACTCATCCAGATGCTTTGAAGGCTGGGTTGGCTGAGTTCATTTCCACGTTGATTTTTGTCTTTGCTGGCCAAGGTTCCGGCATCGCCTTCAGTAAGCTTACCGAAGGCGGCGCCACCACTCCTGCCGGTCTCATCTCCGCATCCATCGCCCATGCCTTTGCCCTTTTTGTTGCCGTCTCCGTCGGTGCCAACATCTCTGGTGGCCATGTCAACCCCGCCGTTACCTTCGGTGCCTTCGTTGGCGGTAACATCACCCTCTTACGTGGCATCATCTACTGGATTGCCCAACTCCTCGGATCCGTCGTCGCATGCTTGCTCCTTAAATTCGTCACCGGCTTGTTATTAACCGATGTTGAGGGTTTTGTTCCTCCCACCGGAAGCTTCGCTCTATCAGCTGGAGTTGGTGAAATAAACGCCTTCGTTTTCGAGATCGTGATGACATTCGGATTGGTGTACACAGTGTACGCGACCGCAGTGGATCCCAAGAAGGGCAGTTTGGGAACTATTGCACCCATCGCAATCGGTTTCATCGTCGGAGCTAACATTTTGGCCGGAGGAGCATTCACCGGAGCATCCATGAACCCAGCCGTGGCCTTCGGTCCATCAGTAGTGAGCTGGTCATGGAGCAGCCACTGGGTCTACTGGGCCGGGCCTCTGATCGGAGGTGGGCTTGCTGGACTCATCTACGAGTTCATCTTCATTTCCAGCACCCACGAGCAGCTCCCAACCACCGACTACTAAGTCAATCACCACAACCCACGGCGGCGGCGACGGAGGCCACTTTTTTTCTCTCCTTAATTTGGTGTATTTGGTTTTGTTAATTTTCTTTTTATCGGACGGGTTTGATCGTGTCTCATTTGTGACCGTTGATCTTTTCATCATCTTTAATTTGTGGTCGTCGTCGTGTGCAGTGCCTGGACCTACGATGATTTGATTGAAGATGAAAATCAGGGCCAGGTTGTAATTTATGTTATTCTGCTCCTGTCTGTACGTGAATTATTTTCAAGTCTTTTTTCTTCTTTGTCCATTTCGATAATTTTCTAAATTTAAGATTTTCTTTTCCTCCTTTCCATTTTCTGAATAATGCAGTGGGACCCATTTTCCCCATCCCGGAATAACTTTATAATAATAATCCCAACCTTTTATATTTTTCACAAA

Coding sequence (CDS)

ATGCCGATCCGTAACATTGCAATTGGAAGGCCCGAAGAGGCTACTCATCCAGATGCTTTGAAGGCTGGGTTGGCTGAGTTCATTTCCACGTTGATTTTTGTCTTTGCTGGCCAAGGTTCCGGCATCGCCTTCAGTAAGCTTACCGAAGGCGGCGCCACCACTCCTGCCGGTCTCATCTCCGCATCCATCGCCCATGCCTTTGCCCTTTTTGTTGCCGTCTCCGTCGGTGCCAACATCTCTGGTGGCCATGTCAACCCCGCCGTTACCTTCGGTGCCTTCGTTGGCGGTAACATCACCCTCTTACGTGGCATCATCTACTGGATTGCCCAACTCCTCGGATCCGTCGTCGCATGCTTGCTCCTTAAATTCGTCACCGGCTTGTTATTAACCGATGTTGAGGGTTTTGTTCCTCCCACCGGAAGCTTCGCTCTATCAGCTGGAGTTGGTGAAATAAACGCCTTCGTTTTCGAGATCGTGATGACATTCGGATTGGTGTACACAGTGTACGCGACCGCAGTGGATCCCAAGAAGGGCAGTTTGGGAACTATTGCACCCATCGCAATCGGTTTCATCGTCGGAGCTAACATTTTGGCCGGAGGAGCATTCACCGGAGCATCCATGAACCCAGCCGTGGCCTTCGGTCCATCAGTAGTGAGCTGGTCATGGAGCAGCCACTGGGTCTACTGGGCCGGGCCTCTGATCGGAGGTGGGCTTGCTGGACTCATCTACGAGTTCATCTTCATTTCCAGCACCCACGAGCAGCTCCCAACCACCGACTACTAA

Protein sequence

MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLISASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLLLKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAGLIYEFIFISSTHEQLPTTDY
Homology
BLAST of Lsi01G012920 vs. ExPASy Swiss-Prot
Match: P25818 (Aquaporin TIP1-1 OS=Arabidopsis thaliana OX=3702 GN=TIP1-1 PE=1 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.3e-116
Identity = 213/260 (81.92%), Postives = 236/260 (90.77%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRP+EAT PDALKA LAEFISTLIFV AG GSG+AF+KLTE GATTP+GL++
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           A++AHAF LFVAVSVGANISGGHVNPAVTFGAF+GGNITLLRGI+YWIAQLLGSVVACL+
Sbjct: 61  AAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKF TG       G   P  +F LSAGVG +NAFVFEIVMTFGLVYTVYATA+DPK GSL
Sbjct: 121 LKFATG-------GLAVP--AFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAF+GASMNPAVAFGP+VVSW+W++HWVYWAGPL+GGG+AG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYE  FI++THEQLPTTDY
Sbjct: 241 LIYEVFFINTTHEQLPTTDY 251

BLAST of Lsi01G012920 vs. ExPASy Swiss-Prot
Match: Q9FY14 (Probable aquaporin TIP-type OS=Medicago truncatula OX=3880 GN=AQP1 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 4.0e-110
Identity = 210/260 (80.77%), Postives = 226/260 (86.92%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+G P+EATHPD LKAGLAEFIST IFVFAG GSGIA++KLT  GA TPAGLIS
Sbjct: 1   MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGI+Y IAQLLGS+VA  L
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIVYIIAQLLGSIVASAL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           L FVT          VP   +F LS GVG   A V EIVMTFGLVYTVYATAVDPKKG++
Sbjct: 121 LVFVTA-------SSVP---AFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNI 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           G IAPIAIGFIVGANIL GGAFTGASMNPAV+FGP+VVSWSWS+HWVYWAGPLIGGG+AG
Sbjct: 181 GIIAPIAIGFIVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           L+YE +FI+STHEQLPTTDY
Sbjct: 241 LVYEVLFINSTHEQLPTTDY 250

BLAST of Lsi01G012920 vs. ExPASy Swiss-Prot
Match: P50156 (Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 9.7e-109
Identity = 207/260 (79.62%), Postives = 223/260 (85.77%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+G  +E  HP ALKA LAEFISTLIFVFAGQGSG+AFSKLT GGATTPAGLI+
Sbjct: 1   MPIRNIAVGSHQEVYHPGALKAALAEFISTLIFVFAGQGSGMAFSKLTGGGATTPAGLIA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           A++AHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITL RG++YWIAQLLGS VAC L
Sbjct: 61  AAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLFRGLLYWIAQLLGSTVACFL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           L+F TG L          TG+F L+ GV    A V EIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LRFSTGGL---------ATGTFGLT-GVSVWEALVLEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANIL GGAF GASMNPAV+FGP++VSWSW S WVYW GPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILVGGAFDGASMNPAVSFGPALVSWSWESQWVYWVGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           +IYE +FIS THEQLPTTDY
Sbjct: 241 VIYEVLFISHTHEQLPTTDY 250

BLAST of Lsi01G012920 vs. ExPASy Swiss-Prot
Match: Q41963 (Aquaporin TIP1-2 OS=Arabidopsis thaliana OX=3702 GN=TIP1-2 PE=1 SV=2)

HSP 1 Score: 393.3 bits (1009), Expect = 2.2e-108
Identity = 203/262 (77.48%), Postives = 226/262 (86.26%), Query Frame = 0

Query: 1   MPIRNIAIGR-PEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLI 60
           MP RNIAIG   EE  HP+AL+A LAEFISTLIFVFAG GSGIAF+K+T+ GATTP+GL+
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKITDNGATTPSGLV 60

Query: 61  SASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACL 120
           +A++AHAF LFVAVSVGANISGGHVNPAVTFG  +GGNITLLRGI+YWIAQLLGSV AC 
Sbjct: 61  AAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNITLLRGILYWIAQLLGSVAACF 120

Query: 121 LLKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGS 180
           LL F TG           P  +F LSAGVG +NA VFEIVMTFGLVYTVYATAVDPK GS
Sbjct: 121 LLSFATG---------GEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGS 180

Query: 181 LGTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLA 240
           LGTIAPIAIGFIVGANILAGGAF+GASMNPAVAFGP+VVSW+W++HWVYWAGPLIGGGLA
Sbjct: 181 LGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLIGGGLA 240

Query: 241 GLIYEFIFI-SSTHEQLPTTDY 261
           G+IY+F+FI  + HEQLPTTDY
Sbjct: 241 GIIYDFVFIDENAHEQLPTTDY 253

BLAST of Lsi01G012920 vs. ExPASy Swiss-Prot
Match: P42067 (Probable aquaporin TIP-type OS=Medicago sativa OX=3879 GN=MCP1 PE=2 SV=2)

HSP 1 Score: 391.0 bits (1003), Expect = 1.1e-107
Identity = 208/260 (80.00%), Postives = 224/260 (86.15%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+G P+EATHPD LKAGLAEFIST IFVFAG GSGIA++KLT  GA TPAGLIS
Sbjct: 1   MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAV FGAFVGGNITLLRGI+Y IAQLLGS+V   L
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAV-FGAFVGGNITLLRGIVYIIAQLLGSIVCSAL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           L FVT          VP   +F LS GVG   A V EIVMTFGLVYTVYATAVDPKKG++
Sbjct: 121 LVFVTA-------SSVP---AFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNI 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           G IAPIAIGFIVGANIL GGAFTGASMNPAV+FGP+VVSWSWS+HWVYWAGPLIGGG+AG
Sbjct: 181 GIIAPIAIGFIVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           L+YE +FI+STHEQLPTTDY
Sbjct: 241 LVYEVLFINSTHEQLPTTDY 249

BLAST of Lsi01G012920 vs. ExPASy TrEMBL
Match: U3R786 (Aquaporin TIP1-1 OS=Cucumis melo OX=3656 GN=LOC103500838 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 8.2e-127
Identity = 242/260 (93.08%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLT  GA TPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSF LSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFGLSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW SHWVYWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWVYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLPTTDY
Sbjct: 241 LIYEFIFISNSHEQLPTTDY 250

BLAST of Lsi01G012920 vs. ExPASy TrEMBL
Match: A0A0A0KF67 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448110 PE=3 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 8.2e-127
Identity = 241/260 (92.69%), Postives = 246/260 (94.62%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAF+KLT  GA TPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW SHW+YWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWIYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLPTTDY
Sbjct: 241 LIYEFIFISNSHEQLPTTDY 250

BLAST of Lsi01G012920 vs. ExPASy TrEMBL
Match: A0A6J1IRX0 (aquaporin TIP1-1 OS=Cucurbita maxima OX=3661 GN=LOC111478777 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.8e-126
Identity = 240/260 (92.31%), Postives = 247/260 (95.00%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT+GGA TPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW +HWVYWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLPTTDY
Sbjct: 241 LIYEFIFISNSHEQLPTTDY 250

BLAST of Lsi01G012920 vs. ExPASy TrEMBL
Match: A0A6J1HEF4 (aquaporin TIP1-1-like OS=Cucurbita moschata OX=3662 GN=LOC111463217 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.3e-126
Identity = 239/260 (91.92%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRPEEAT PDALKAGLAEFISTLIFVFAGQGSGIAF KLT+GGA TPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATRPDALKAGLAEFISTLIFVFAGQGSGIAFGKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGEVNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW +HWVYWAGPL+GGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLVGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS+THEQLP T+Y
Sbjct: 241 LIYEFIFISNTHEQLPVTEY 250

BLAST of Lsi01G012920 vs. ExPASy TrEMBL
Match: A0A6J1ED08 (aquaporin TIP1-1 OS=Cucurbita moschata OX=3662 GN=LOC111433053 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.3e-126
Identity = 239/260 (91.92%), Postives = 247/260 (95.00%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT+GGA TPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW +HWVYWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLP+TDY
Sbjct: 241 LIYEFIFISNSHEQLPSTDY 250

BLAST of Lsi01G012920 vs. NCBI nr
Match: XP_004143284.1 (aquaporin TIP1-1 [Cucumis sativus] >KGN48203.1 hypothetical protein Csa_002806 [Cucumis sativus])

HSP 1 Score: 463.0 bits (1190), Expect = 1.7e-126
Identity = 241/260 (92.69%), Postives = 246/260 (94.62%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAF+KLT  GA TPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW SHW+YWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWIYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLPTTDY
Sbjct: 241 LIYEFIFISNSHEQLPTTDY 250

BLAST of Lsi01G012920 vs. NCBI nr
Match: NP_001284655.1 (aquaporin TIP1-1 [Cucumis melo] >AGW99230.1 aquaporin TIP1-1 [Cucumis melo])

HSP 1 Score: 463.0 bits (1190), Expect = 1.7e-126
Identity = 242/260 (93.08%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLT  GA TPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSF LSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFGLSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW SHWVYWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWVYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLPTTDY
Sbjct: 241 LIYEFIFISNSHEQLPTTDY 250

BLAST of Lsi01G012920 vs. NCBI nr
Match: XP_022978995.1 (aquaporin TIP1-1 [Cucurbita maxima] >XP_023544236.1 aquaporin TIP1-1 [Cucurbita pepo subsp. pepo] >KAG6604390.1 Aquaporin TIP1-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034540.1 Aquaporin TIP1-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.8 bits (1187), Expect = 3.8e-126
Identity = 240/260 (92.31%), Postives = 247/260 (95.00%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT+GGA TPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW +HWVYWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLPTTDY
Sbjct: 241 LIYEFIFISNSHEQLPTTDY 250

BLAST of Lsi01G012920 vs. NCBI nr
Match: XP_022925724.1 (aquaporin TIP1-1 [Cucurbita moschata])

HSP 1 Score: 460.3 bits (1183), Expect = 1.1e-125
Identity = 239/260 (91.92%), Postives = 247/260 (95.00%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT+GGA TPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW +HWVYWAGPLIGGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS++HEQLP+TDY
Sbjct: 241 LIYEFIFISNSHEQLPSTDY 250

BLAST of Lsi01G012920 vs. NCBI nr
Match: XP_022962846.1 (aquaporin TIP1-1-like [Cucurbita moschata] >KAG7027237.1 Aquaporin TIP1-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 460.3 bits (1183), Expect = 1.1e-125
Identity = 239/260 (91.92%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRPEEAT PDALKAGLAEFISTLIFVFAGQGSGIAF KLT+GGA TPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATRPDALKAGLAEFISTLIFVFAGQGSGIAFGKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKFVTGL          PTGSFALSAGVGE+NAFVFEIVMTFGLVYTVYATAVDPKKGSL
Sbjct: 121 LKFVTGL----------PTGSFALSAGVGEVNAFVFEIVMTFGLVYTVYATAVDPKKGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSW +HWVYWAGPL+GGGLAG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLVGGGLAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYEFIFIS+THEQLP T+Y
Sbjct: 241 LIYEFIFISNTHEQLPVTEY 250

BLAST of Lsi01G012920 vs. TAIR 10
Match: AT2G36830.1 (gamma tonoplast intrinsic protein )

HSP 1 Score: 420.6 bits (1080), Expect = 9.0e-118
Identity = 213/260 (81.92%), Postives = 236/260 (90.77%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPIRNIAIGRP+EAT PDALKA LAEFISTLIFV AG GSG+AF+KLTE GATTP+GL++
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           A++AHAF LFVAVSVGANISGGHVNPAVTFGAF+GGNITLLRGI+YWIAQLLGSVVACL+
Sbjct: 61  AAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LKF TG       G   P  +F LSAGVG +NAFVFEIVMTFGLVYTVYATA+DPK GSL
Sbjct: 121 LKFATG-------GLAVP--AFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSL 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           GTIAPIAIGFIVGANILAGGAF+GASMNPAVAFGP+VVSW+W++HWVYWAGPL+GGG+AG
Sbjct: 181 GTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAG 240

Query: 241 LIYEFIFISSTHEQLPTTDY 261
           LIYE  FI++THEQLPTTDY
Sbjct: 241 LIYEVFFINTTHEQLPTTDY 251

BLAST of Lsi01G012920 vs. TAIR 10
Match: AT3G26520.1 (tonoplast intrinsic protein 2 )

HSP 1 Score: 393.3 bits (1009), Expect = 1.5e-109
Identity = 203/262 (77.48%), Postives = 226/262 (86.26%), Query Frame = 0

Query: 1   MPIRNIAIGR-PEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLI 60
           MP RNIAIG   EE  HP+AL+A LAEFISTLIFVFAG GSGIAF+K+T+ GATTP+GL+
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKITDNGATTPSGLV 60

Query: 61  SASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACL 120
           +A++AHAF LFVAVSVGANISGGHVNPAVTFG  +GGNITLLRGI+YWIAQLLGSV AC 
Sbjct: 61  AAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNITLLRGILYWIAQLLGSVAACF 120

Query: 121 LLKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGS 180
           LL F TG           P  +F LSAGVG +NA VFEIVMTFGLVYTVYATAVDPK GS
Sbjct: 121 LLSFATG---------GEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGS 180

Query: 181 LGTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLA 240
           LGTIAPIAIGFIVGANILAGGAF+GASMNPAVAFGP+VVSW+W++HWVYWAGPLIGGGLA
Sbjct: 181 LGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLIGGGLA 240

Query: 241 GLIYEFIFI-SSTHEQLPTTDY 261
           G+IY+F+FI  + HEQLPTTDY
Sbjct: 241 GIIYDFVFIDENAHEQLPTTDY 253

BLAST of Lsi01G012920 vs. TAIR 10
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3 )

HSP 1 Score: 352.1 bits (902), Expect = 3.9e-97
Identity = 181/261 (69.35%), Postives = 210/261 (80.46%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLIS 60
           MPI  IAIG P EA+ PDA++A  AEF S +IFVFAGQGSG+A+ KLT  G  TPAGL++
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGSGMAYGKLTGDGPATPAGLVA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120
           AS++HAFALFVAVSVGAN+SGGHVNPAVTFGAF+GGNITLLR I+YWIAQLLG+VVACLL
Sbjct: 61  ASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNITLLRAILYWIAQLLGAVVACLL 120

Query: 121 LKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSL 180
           LK  TG +          T +F+LS GV   NA VFEIVMTFGLVYTVYATAVDPKKG +
Sbjct: 121 LKVSTGGM---------ETAAFSLSYGVTPWNAVVFEIVMTFGLVYTVYATAVDPKKGDI 180

Query: 181 GTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAG 240
           G IAP+AIG IVGANIL GGAF GASMNPAV+FGP+VVSW W++HWVYW GP IG  +A 
Sbjct: 181 GIIAPLAIGLIVGANILVGGAFDGASMNPAVSFGPAVVSWIWTNHWVYWVGPFIGAAIAA 240

Query: 241 LIYEFIFISST-HEQLPTTDY 261
           ++Y+ IFI S  HE LP+ D+
Sbjct: 241 IVYDTIFIGSNGHEPLPSNDF 252

BLAST of Lsi01G012920 vs. TAIR 10
Match: AT3G16240.1 (delta tonoplast integral protein )

HSP 1 Score: 291.6 bits (745), Expect = 6.3e-79
Identity = 157/256 (61.33%), Postives = 189/256 (73.83%), Query Frame = 0

Query: 6   IAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTEGGATTPAGLISASIAH 65
           +A G  +++    +L+A LAEFISTL+FVFAG GS IA++KLT   A    GL++ ++ H
Sbjct: 4   VAFGSFDDSFSLASLRAYLAEFISTLLFVFAGVGSAIAYAKLTSDAALDTPGLVAIAVCH 63

Query: 66  AFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLLLKFVT 125
            FALFVAV++GANISGGHVNPAVTFG  VGG IT++ G+ YWIAQLLGS  AC LLK+VT
Sbjct: 64  GFALFVAVAIGANISGGHVNPAVTFGLAVGGQITVITGVFYWIAQLLGSTAACFLLKYVT 123

Query: 126 GLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAP 185
           G       G   PT S A  AG+G I   V EI++TF LVYTVYATA DPKKGSLGTIAP
Sbjct: 124 G-------GLAVPTHSVA--AGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIAP 183

Query: 186 IAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGLAGLIYEF 245
           +AIG IVGANILA G F+G SMNPA +FGP+V +  +S HWVYW GPLIGGGLAGLIY  
Sbjct: 184 LAIGLIVGANILAAGPFSGGSMNPARSFGPAVAAGDFSGHWVYWVGPLIGGGLAGLIYGN 243

Query: 246 IFI-SSTHEQLPTTDY 261
           +F+ SS H  L + D+
Sbjct: 244 VFMGSSEHVPLASADF 250

BLAST of Lsi01G012920 vs. TAIR 10
Match: AT1G73190.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 2.4e-78
Identity = 150/271 (55.35%), Postives = 186/271 (68.63%), Query Frame = 0

Query: 4   RNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKL-----TEGGATTPAGL 63
           R    GR +EATHPD+++A LAEF+ST +FVFA +GS ++  KL        G  TP GL
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKLYWEHAAHAGTNTPGGL 66

Query: 64  ISASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVAC 123
           I  ++AHAFALF AVS   N+SGGHVNPAVTFGA VGG +T +R I YWIAQLLG+++AC
Sbjct: 67  ILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRVTAIRAIYYWIAQLLGAILAC 126

Query: 124 LLLKFVTGLLLTDVEGFVPPTGSFALSAGVGEINAFVFEIVMTFGLVYTVYATAVDPKKG 183
           LLL+  T          + P G F L++GVG +N  V EI++TFGLVY VY+T +DPK+G
Sbjct: 127 LLLRLTT--------NGMRPVG-FRLASGVGAVNGLVLEIILTFGLVYVVYSTLIDPKRG 186

Query: 184 SLGTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWSSHWVYWAGPLIGGGL 243
           SLG IAP+AIG IVGANIL GG F+GASMNPA AFGP++V W W  HW+YW GP IG  L
Sbjct: 187 SLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWIYWVGPFIGSAL 246

Query: 244 AGLIYEFIFISS---------THEQLPTTDY 261
           A LIYE++ I +          H+ L   DY
Sbjct: 247 AALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P258181.3e-11681.92Aquaporin TIP1-1 OS=Arabidopsis thaliana OX=3702 GN=TIP1-1 PE=1 SV=1[more]
Q9FY144.0e-11080.77Probable aquaporin TIP-type OS=Medicago truncatula OX=3880 GN=AQP1 PE=1 SV=1[more]
P501569.7e-10979.62Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP1-1 PE=... [more]
Q419632.2e-10877.48Aquaporin TIP1-2 OS=Arabidopsis thaliana OX=3702 GN=TIP1-2 PE=1 SV=2[more]
P420671.1e-10780.00Probable aquaporin TIP-type OS=Medicago sativa OX=3879 GN=MCP1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
U3R7868.2e-12793.08Aquaporin TIP1-1 OS=Cucumis melo OX=3656 GN=LOC103500838 PE=2 SV=1[more]
A0A0A0KF678.2e-12792.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448110 PE=3 SV=1[more]
A0A6J1IRX01.8e-12692.31aquaporin TIP1-1 OS=Cucurbita maxima OX=3661 GN=LOC111478777 PE=3 SV=1[more]
A0A6J1HEF45.3e-12691.92aquaporin TIP1-1-like OS=Cucurbita moschata OX=3662 GN=LOC111463217 PE=3 SV=1[more]
A0A6J1ED085.3e-12691.92aquaporin TIP1-1 OS=Cucurbita moschata OX=3662 GN=LOC111433053 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143284.11.7e-12692.69aquaporin TIP1-1 [Cucumis sativus] >KGN48203.1 hypothetical protein Csa_002806 [... [more]
NP_001284655.11.7e-12693.08aquaporin TIP1-1 [Cucumis melo] >AGW99230.1 aquaporin TIP1-1 [Cucumis melo][more]
XP_022978995.13.8e-12692.31aquaporin TIP1-1 [Cucurbita maxima] >XP_023544236.1 aquaporin TIP1-1 [Cucurbita ... [more]
XP_022925724.11.1e-12591.92aquaporin TIP1-1 [Cucurbita moschata][more]
XP_022962846.11.1e-12591.92aquaporin TIP1-1-like [Cucurbita moschata] >KAG7027237.1 Aquaporin TIP1-1 [Cucur... [more]
Match NameE-valueIdentityDescription
AT2G36830.19.0e-11881.92gamma tonoplast intrinsic protein [more]
AT3G26520.11.5e-10977.48tonoplast intrinsic protein 2 [more]
AT4G01470.13.9e-9769.35tonoplast intrinsic protein 1;3 [more]
AT3G16240.16.3e-7961.33delta tonoplast integral protein [more]
AT1G73190.12.4e-7855.35Aquaporin-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 156..174
score: 42.65
coord: 102..121
score: 38.3
coord: 226..246
score: 43.5
coord: 21..40
score: 45.07
coord: 65..89
score: 50.93
coord: 189..211
score: 45.93
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 25..243
e-value: 4.6E-52
score: 175.0
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 14..243
e-value: 4.3E-75
score: 252.3
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 21..246
e-value: 7.12E-61
score: 189.772
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 4..260
e-value: 1.0E-76
score: 259.3
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 13..255
IPR034294Aquaporin transporterPANTHERPTHR45665AQUAPORIN-8coord: 1..260
NoneNo IPR availablePANTHERPTHR45665:SF2AQUAPORIN TIP1-1coord: 1..260
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 83..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G012920.1Lsi01G012920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity