Lsi01G008040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G008040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmitochondrial uncoupling protein 5-like
Locationchr01: 6354957 .. 6355931 (-)
RNA-Seq ExpressionLsi01G008040
SyntenyLsi01G008040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTCAAGGGATTTCTCGAAGGTGGAATTGCTTCCATTGTTGCCGGCTGTTCCACCCACCCGCTTGATCTCATCAAGGTCCGAATGCAACTTCAGGGTGAGACTCCTGCTCCTACGGCCGCGGTTCATAATCTCCGCCCTGCTCTCGCTTTTCAAACCACCTCTGTAACTGCACCGAGGTCTATGAATATTCCTCCGCCGCCGCCTCCGGCAGCGCGTGTTGGTCCAATCGCTGTTGGGGTTCGTATTGTGCAGCAGGAAGGAGTAGCGGCTCTCTTCTCTGGCGTTTCCGCCACCGTTCTCCGACAGACTCTGTACTCGACTACCCGTATGGGGCTTTACGATATTCTAAAACAGAAGTGGACCGATCAGGATACCGGGAAAATGCCACTCATTCGGAAGATATCGGCCGGTTTGATTGCCGGCGCAGTCGGTGCTGCAGTCGGCAACCCGGCTGATGTAGCGATGGTCCGAATGCAAGCCGACGGGCGGCTACCACTAGCGCAGCGGCGGAACTACAAAAGCGTGGTCGACGCCATAACCCAGATGGCACGTGGCGAAGGCGTGACGAGTCTGTGGCGTGGTTCGTCGTTGACGGTGAATCGTGCGATGCTCGTAACGGCTTCGCAGCTCGCATCGTACGATCAGATCAAGGAGACAATTCTGCAGAAGGGATTGATGAAGGATGGGCTTGGGACACACGTGACGGCGAGTTTCGCAGCCGGATTCGTGGCGGCGGTGGCGTCGAATCCGGTGGACGTGATAAAAACCAGAGTGATGAACATGAAGGTGGAGGCGGGGGCTGCGCCACCGTACTCCGGCGCGTTGGATTGCGCGTTGAAGACGGTACGGGCGGAGGGGCCGATGGCACTATACAAAGGGTTTATTCCGACGATTTCACGGCAGGGACCATTCACGGTGGTGCTGTTTGTGACGCTGGAACAAGTTCGAAAGGTGTTTAAAGATTTCTAA

mRNA sequence

ATGGGTTTCAAGGGATTTCTCGAAGGTGGAATTGCTTCCATTGTTGCCGGCTGTTCCACCCACCCGCTTGATCTCATCAAGGTCCGAATGCAACTTCAGGGTGAGACTCCTGCTCCTACGGCCGCGGTTCATAATCTCCGCCCTGCTCTCGCTTTTCAAACCACCTCTGTAACTGCACCGAGGTCTATGAATATTCCTCCGCCGCCGCCTCCGGCAGCGCGTGTTGGTCCAATCGCTGTTGGGGTTCGTATTGTGCAGCAGGAAGGAGTAGCGGCTCTCTTCTCTGGCGTTTCCGCCACCGTTCTCCGACAGACTCTGTACTCGACTACCCGTATGGGGCTTTACGATATTCTAAAACAGAAGTGGACCGATCAGGATACCGGGAAAATGCCACTCATTCGGAAGATATCGGCCGGTTTGATTGCCGGCGCAGTCGGTGCTGCAGTCGGCAACCCGGCTGATGTAGCGATGGTCCGAATGCAAGCCGACGGGCGGCTACCACTAGCGCAGCGGCGGAACTACAAAAGCGTGGTCGACGCCATAACCCAGATGGCACGTGGCGAAGGCGTGACGAGTCTGTGGCGTGGTTCGTCGTTGACGGTGAATCGTGCGATGCTCGTAACGGCTTCGCAGCTCGCATCGTACGATCAGATCAAGGAGACAATTCTGCAGAAGGGATTGATGAAGGATGGGCTTGGGACACACGTGACGGCGAGTTTCGCAGCCGGATTCGTGGCGGCGGTGGCGTCGAATCCGGTGGACGTGATAAAAACCAGAGTGATGAACATGAAGGTGGAGGCGGGGGCTGCGCCACCGTACTCCGGCGCGTTGGATTGCGCGTTGAAGACGGTACGGGCGGAGGGGCCGATGGCACTATACAAAGGGTTTATTCCGACGATTTCACGGCAGGGACCATTCACGGTGGTGCTGTTTGTGACGCTGGAACAAGTTCGAAAGGTGTTTAAAGATTTCTAA

Coding sequence (CDS)

ATGGGTTTCAAGGGATTTCTCGAAGGTGGAATTGCTTCCATTGTTGCCGGCTGTTCCACCCACCCGCTTGATCTCATCAAGGTCCGAATGCAACTTCAGGGTGAGACTCCTGCTCCTACGGCCGCGGTTCATAATCTCCGCCCTGCTCTCGCTTTTCAAACCACCTCTGTAACTGCACCGAGGTCTATGAATATTCCTCCGCCGCCGCCTCCGGCAGCGCGTGTTGGTCCAATCGCTGTTGGGGTTCGTATTGTGCAGCAGGAAGGAGTAGCGGCTCTCTTCTCTGGCGTTTCCGCCACCGTTCTCCGACAGACTCTGTACTCGACTACCCGTATGGGGCTTTACGATATTCTAAAACAGAAGTGGACCGATCAGGATACCGGGAAAATGCCACTCATTCGGAAGATATCGGCCGGTTTGATTGCCGGCGCAGTCGGTGCTGCAGTCGGCAACCCGGCTGATGTAGCGATGGTCCGAATGCAAGCCGACGGGCGGCTACCACTAGCGCAGCGGCGGAACTACAAAAGCGTGGTCGACGCCATAACCCAGATGGCACGTGGCGAAGGCGTGACGAGTCTGTGGCGTGGTTCGTCGTTGACGGTGAATCGTGCGATGCTCGTAACGGCTTCGCAGCTCGCATCGTACGATCAGATCAAGGAGACAATTCTGCAGAAGGGATTGATGAAGGATGGGCTTGGGACACACGTGACGGCGAGTTTCGCAGCCGGATTCGTGGCGGCGGTGGCGTCGAATCCGGTGGACGTGATAAAAACCAGAGTGATGAACATGAAGGTGGAGGCGGGGGCTGCGCCACCGTACTCCGGCGCGTTGGATTGCGCGTTGAAGACGGTACGGGCGGAGGGGCCGATGGCACTATACAAAGGGTTTATTCCGACGATTTCACGGCAGGGACCATTCACGGTGGTGCTGTTTGTGACGCTGGAACAAGTTCGAAAGGTGTTTAAAGATTTCTAA

Protein sequence

MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAPRSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQKWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDAITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKVFKDF
Homology
BLAST of Lsi01G008040 vs. ExPASy Swiss-Prot
Match: Q9SJY5 (Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.2e-135
Identity = 254/324 (78.40%), Postives = 285/324 (87.96%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF EGGIASIVAGCSTHPLDLIKVRMQLQGE+ AP     NLRPALAFQT++    
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGES-APIQT--NLRPALAFQTST---- 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
            ++N PP      RVG I VG R++++EG+ ALFSGVSATVLRQTLYSTTRMGLYDI+K 
Sbjct: 61  -TVNAPP-----LRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKG 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           +WTD +T  MPL++KI AG IAGA+GAAVGNPADVAMVRMQADGRLPL  RRNYKSV+DA
Sbjct: 121 EWTDPETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQM RGEGVTSLWRGSSLT+NRAMLVT+SQLASYD +KETIL+KGL+KDGLGTHV+ASF
Sbjct: 181 ITQMIRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV AG APPY GA+DCALKTV+AEG M+LYKGFIPT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQ PFTVVLFVTLEQV+K+FKD+
Sbjct: 301 SRQAPFTVVLFVTLEQVKKLFKDY 311

BLAST of Lsi01G008040 vs. ExPASy Swiss-Prot
Match: Q9SB52 (Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.5e-124
Identity = 234/324 (72.22%), Postives = 267/324 (82.41%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG K F+EGGIAS++AGCSTHPLDLIKVR+QL GE P+ T  V  LRPALAF  +S  A 
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPS-TTTVTLLRPALAFPNSSPAAF 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
                  P     +VGPI++G+ IV+ EG AALFSGVSAT+LRQTLYSTTRMGLY++LK 
Sbjct: 61  LETTSSVP-----KVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKN 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTD ++GK+ L RKI AGL+AG +GAAVGNPADVAMVRMQADGRLPLAQRRNY  V DA
Sbjct: 121 KWTDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           I  M +GEGVTSLWRGS+LT+NRAM+VTA+QLASYDQ KE IL+ G+M DGLGTHV ASF
Sbjct: 181 IRSMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV A     Y GA DCA+KTV+AEG MALYKGF+PT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
            RQGPFTVVLFVTLEQVRK+ +DF
Sbjct: 301 CRQGPFTVVLFVTLEQVRKLLRDF 313

BLAST of Lsi01G008040 vs. ExPASy Swiss-Prot
Match: Q9FY68 (Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 6.9e-104
Identity = 213/339 (62.83%), Postives = 250/339 (73.75%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGE-------TPAPTAAV-HNL-----R 60
           MGFK FLEGGIA+I+AG  THPLDLIKVRMQLQGE        P P  ++ HNL     R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFQTT--SVTAPRSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQT 120
           P  A  +   S++        P     + + P AVG  IV+ EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDILKQKWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK++WTDQ TG  PL+ KI+AGLIAGAVG+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPLAQRRNYKSVVDAITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQK 240
           LPL +RRNYKSVVDAI ++AR EGV+SLWRGS LTVNRAM+VTASQLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 GL-MKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 RAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKVFKD 324
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR + KD
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVRGLLKD 334

BLAST of Lsi01G008040 vs. ExPASy Swiss-Prot
Match: Q54PY7 (Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium discoideum OX=44689 GN=ucpC PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 9.1e-64
Identity = 140/319 (43.89%), Postives = 191/319 (59.87%), Query Frame = 0

Query: 4   KGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAPRSM 63
           K F+ GG+A +++   THP+D +KVRMQLQGE                   T V   R  
Sbjct: 26  KQFVIGGLAGMLSSAFTHPIDSLKVRMQLQGE------------------GTGVGPKR-- 85

Query: 64  NIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQKWT 123
                       G + + V I Q EG   L+ G+SA++LRQ  Y+TTR GLYD++K    
Sbjct: 86  ------------GALKMLVHINQTEGFFTLYKGLSASLLRQATYTTTRFGLYDLIKD-IV 145

Query: 124 DQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDAITQ 183
            +D   +P  +KI  G+++GA GA VG PAD+ MVRMQADG+LP   RRNYK+V D I +
Sbjct: 146 AKDDKPLPFTQKIMVGMLSGAGGAIVGTPADLTMVRMQADGKLPFNLRRNYKNVFDGIFR 205

Query: 184 MARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAAG 243
           +++ EG+ SLW+G S  + RAM +TA Q++SYDQ K+ +L  G   D + TH+ AS  A 
Sbjct: 206 ISKEEGIISLWKGCSPNLIRAMFMTAGQVSSYDQTKQLMLASGYFHDDIKTHLIASTTAA 265

Query: 244 FVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTISRQ 303
           FVAAVA++P+DVIKTR+MN          Y G  DC  KT+RAEG  A YKGF P   R 
Sbjct: 266 FVAAVATSPLDVIKTRIMNSPKTVTGELQYKGTFDCLSKTLRAEGFKAFYKGFNPYFMRL 311

Query: 304 GPFTVVLFVTLEQVRKVFK 323
           GP T++ F+ +EQ+  ++K
Sbjct: 326 GPQTILTFIFVEQLNILWK 311

BLAST of Lsi01G008040 vs. ExPASy Swiss-Prot
Match: Q9CR62 (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Mus musculus OX=10090 GN=Slc25a11 PE=1 SV=3)

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-57
Identity = 133/317 (41.96%), Postives = 181/317 (57.10%), Query Frame = 0

Query: 6   FLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAPRSMNI 65
           FL GG+A + A     PLDL+K RMQL GE  A T        AL               
Sbjct: 25  FLFGGLAGMGATVFVQPLDLVKNRMQLSGE-GAKTREYKTSFHAL--------------- 84

Query: 66  PPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQKWTDQ 125
                             I++ EG+  +++G+SA +LRQ  Y+TTR+G+Y +L ++ T  
Sbjct: 85  ----------------TSILKTEGLKGIYTGLSAGLLRQATYTTTRLGIYTVLFERLTGA 144

Query: 126 DTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDAITQMA 185
           D      + K   G+ AGA GA VG PA+VA++RM ADGRLP  QRR YK+V +A+ ++A
Sbjct: 145 DGTPPGFLLKALIGMTAGATGAFVGTPAEVALIRMTADGRLPADQRRGYKNVFNALVRIA 204

Query: 186 RGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAAGFV 245
           R EGV +LWRG   T+ RA++V A+QLASY Q K+ +L  G   D +  H  AS  +G V
Sbjct: 205 REEGVPTLWRGCIPTMARAVVVNAAQLASYSQSKQFLLDSGYFSDNILCHFCASMISGLV 264

Query: 246 AAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTISRQGP 305
              AS PVD++KTR+ NM++  G  P Y   LD  LK VR EG  +L+KGF P  +R GP
Sbjct: 265 TTAASMPVDIVKTRIQNMRMIDG-KPEYKNGLDVLLKVVRYEGFFSLWKGFTPYYARLGP 308

Query: 306 FTVVLFVTLEQVRKVFK 323
            TV+ F+ LEQ+ K +K
Sbjct: 325 HTVLTFIFLEQMNKAYK 308

BLAST of Lsi01G008040 vs. ExPASy TrEMBL
Match: A0A5A7T2E3 (Mitochondrial uncoupling protein 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003180 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.0e-172
Identity = 319/324 (98.46%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAV+NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVNNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RS+NIPPPPPP ARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ
Sbjct: 61  RSINIPPPPPPTARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKVFKDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVFKDF 324

BLAST of Lsi01G008040 vs. ExPASy TrEMBL
Match: A0A1S3B1F2 (mitochondrial uncoupling protein 5-like OS=Cucumis melo OX=3656 GN=LOC103484939 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.0e-172
Identity = 319/324 (98.46%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAV+NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVNNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RS+NIPPPPPP ARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ
Sbjct: 61  RSINIPPPPPPTARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKVFKDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVFKDF 324

BLAST of Lsi01G008040 vs. ExPASy TrEMBL
Match: A0A0A0KIS8 (Mitochondrial dicarboxylate carrier protein OS=Cucumis sativus OX=3659 GN=Csa_6G499090 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.0e-171
Identity = 317/324 (97.84%), Postives = 321/324 (99.07%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           +S+NIPPPPPP ARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ
Sbjct: 61  KSINIPPPPPPPARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. ExPASy TrEMBL
Match: A0A6J1GDC4 (mitochondrial uncoupling protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452954 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.7e-166
Identity = 308/324 (95.06%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGE PAPT AV NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGEAPAPTGAVQNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGP+AVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGK+PL+RKISAGLIAGA+GAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQM RGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMVRGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAGAA PYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. ExPASy TrEMBL
Match: A0A6J1IN72 (mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111477955 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.6e-166
Identity = 307/324 (94.75%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPT AV NLRPALAFQT SVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTGAVQNLRPALAFQTNSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGP+AVGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQKEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGK+PL+RKISAGLIAGA+GAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAA PYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEEGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. NCBI nr
Match: XP_008440559.1 (PREDICTED: mitochondrial uncoupling protein 5-like [Cucumis melo] >KAA0036336.1 mitochondrial uncoupling protein 5-like [Cucumis melo var. makuwa] >TYK12730.1 mitochondrial uncoupling protein 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 612.8 bits (1579), Expect = 1.7e-171
Identity = 319/324 (98.46%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAV+NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVNNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RS+NIPPPPPP ARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ
Sbjct: 61  RSINIPPPPPPTARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKVFKDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVFKDF 324

BLAST of Lsi01G008040 vs. NCBI nr
Match: XP_004143448.1 (mitochondrial uncoupling protein 5 [Cucumis sativus] >KGN48714.1 hypothetical protein Csa_002838 [Cucumis sativus])

HSP 1 Score: 610.5 bits (1573), Expect = 8.2e-171
Identity = 317/324 (97.84%), Postives = 321/324 (99.07%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           +S+NIPPPPPP ARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ
Sbjct: 61  KSINIPPPPPPPARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. NCBI nr
Match: XP_038883604.1 (mitochondrial uncoupling protein 5-like [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.4e-170
Identity = 316/324 (97.53%), Postives = 320/324 (98.77%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RSMNIPPPPPPAARVGPI VGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ
Sbjct: 61  RSMNIPPPPPPAARVGPITVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGKM L+RKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP+AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMSLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPVAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAG+APPYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGSAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. NCBI nr
Match: KAG6604059.1 (Mitochondrial uncoupling protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 310/324 (95.68%), Postives = 317/324 (97.84%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPT AV NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTGAVQNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGP+AVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGK+PL+RKISAGLIAGA+GAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAGAA PYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. NCBI nr
Match: XP_023544374.1 (mitochondrial uncoupling protein 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596.7 bits (1537), Expect = 1.2e-166
Identity = 309/324 (95.37%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGETPAPT AV NLRPALAFQT SVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTGAVQNLRPALAFQTNSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGP+AVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTDQDTGK+PL+RKISAGLIAGA+GAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVEAGAA PYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Lsi01G008040 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 484.2 bits (1245), Expect = 8.3e-137
Identity = 254/324 (78.40%), Postives = 285/324 (87.96%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG KGF EGGIASIVAGCSTHPLDLIKVRMQLQGE+ AP     NLRPALAFQT++    
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGES-APIQT--NLRPALAFQTST---- 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
            ++N PP      RVG I VG R++++EG+ ALFSGVSATVLRQTLYSTTRMGLYDI+K 
Sbjct: 61  -TVNAPP-----LRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKG 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           +WTD +T  MPL++KI AG IAGA+GAAVGNPADVAMVRMQADGRLPL  RRNYKSV+DA
Sbjct: 121 EWTDPETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQM RGEGVTSLWRGSSLT+NRAMLVT+SQLASYD +KETIL+KGL+KDGLGTHV+ASF
Sbjct: 181 ITQMIRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV AG APPY GA+DCALKTV+AEG M+LYKGFIPT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQ PFTVVLFVTLEQV+K+FKD+
Sbjct: 301 SRQAPFTVVLFVTLEQVKKLFKDY 311

BLAST of Lsi01G008040 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 446.0 bits (1146), Expect = 2.5e-125
Identity = 234/324 (72.22%), Postives = 267/324 (82.41%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60
           MG K F+EGGIAS++AGCSTHPLDLIKVR+QL GE P+ T  V  LRPALAF  +S  A 
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPS-TTTVTLLRPALAFPNSSPAAF 60

Query: 61  RSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120
                  P     +VGPI++G+ IV+ EG AALFSGVSAT+LRQTLYSTTRMGLY++LK 
Sbjct: 61  LETTSSVP-----KVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKN 120

Query: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180
           KWTD ++GK+ L RKI AGL+AG +GAAVGNPADVAMVRMQADGRLPLAQRRNY  V DA
Sbjct: 121 KWTDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           I  M +GEGVTSLWRGS+LT+NRAM+VTA+QLASYDQ KE IL+ G+M DGLGTHV ASF
Sbjct: 181 IRSMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV A     Y GA DCA+KTV+AEG MALYKGF+PT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
            RQGPFTVVLFVTLEQVRK+ +DF
Sbjct: 301 CRQGPFTVVLFVTLEQVRKLLRDF 313

BLAST of Lsi01G008040 vs. TAIR 10
Match: AT5G09470.1 (dicarboxylate carrier 3 )

HSP 1 Score: 378.6 bits (971), Expect = 4.9e-105
Identity = 213/339 (62.83%), Postives = 250/339 (73.75%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGE-------TPAPTAAV-HNL-----R 60
           MGFK FLEGGIA+I+AG  THPLDLIKVRMQLQGE        P P  ++ HNL     R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFQTT--SVTAPRSMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQT 120
           P  A  +   S++        P     + + P AVG  IV+ EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDILKQKWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK++WTDQ TG  PL+ KI+AGLIAGAVG+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPLAQRRNYKSVVDAITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQK 240
           LPL +RRNYKSVVDAI ++AR EGV+SLWRGS LTVNRAM+VTASQLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 GL-MKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 RAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKVFKD 324
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR + KD
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVRGLLKD 334

BLAST of Lsi01G008040 vs. TAIR 10
Match: AT5G19760.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.8e-52
Identity = 122/325 (37.54%), Postives = 177/325 (54.46%), Query Frame = 0

Query: 4   KGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAPRSM 63
           K F+ GG + ++A C   P+D+IKVR+QL   + A                         
Sbjct: 16  KPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAA------------------------- 75

Query: 64  NIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQKWT 123
                          ++   +++ EGV A + G+SA +LRQ  Y+T R+G + +L  K  
Sbjct: 76  ---------------SITTNMLKNEGVGAFYKGLSAGLLRQATYTTARLGSFKLLTAKAI 135

Query: 124 DQDTGK-MPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDAIT 183
           + + GK +PL +K   GL AGA+GA VG+PAD+A++RMQAD  LPLAQRRNY +   A+T
Sbjct: 136 ESNDGKPLPLYQKALCGLTAGAIGACVGSPADLALIRMQADNTLPLAQRRNYTNAFHALT 195

Query: 184 QMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLG-----THVT 243
           +++  EGV +LW+G   TV RAM +    LASYDQ  E       M+D LG     T V 
Sbjct: 196 RISADEGVLALWKGCGPTVVRAMALNMGMLASYDQSAE------YMRDNLGFGEMSTVVG 255

Query: 244 ASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFI 303
           AS  +GF AA  S P D +KT++  M+ +A    PY+G+LDCA+KT++  GP+  Y GF 
Sbjct: 256 ASAVSGFCAAACSLPFDFVKTQIQKMQPDAQGKYPYTGSLDCAMKTLKEGGPLKFYSGFP 294

Query: 304 PTISRQGPFTVVLFVTLEQVRKVFK 323
               R  P  ++ ++ L Q+ K  K
Sbjct: 316 VYCVRIAPHVMMTWIFLNQITKFQK 294

BLAST of Lsi01G008040 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 124/321 (38.63%), Postives = 176/321 (54.83%), Query Frame = 0

Query: 6   FLEGGIASIVAGC----STHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAPR 65
           FLE  I S  A C     T PLD  KVR+QLQ +   PT    NL               
Sbjct: 12  FLETFICSAFAACFAELCTIPLDTAKVRLQLQRK--IPTGDGENL--------------- 71

Query: 66  SMNIPPPPPPAARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQK 125
                  P     +G +A    I ++EG++ L+ GV A + RQ +Y   R+GLY+ +K  
Sbjct: 72  -------PKYRGSIGTLAT---IAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTL 131

Query: 126 WTDQD-TGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 185
               D  G +PL +KI A L+ GA+   V NP D+  VR+Q++G+LP    R Y   VDA
Sbjct: 132 LVGSDFIGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDA 191

Query: 186 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 245
              + + EGV++LW G    + R  +V A++LASYDQIKETI++    +D + TH+ A  
Sbjct: 192 YFTIVKLEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGL 251

Query: 246 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 305
           AAGF A    +P+DV+K+R+M           Y   +DC +KT++ EG MA YKGF+P  
Sbjct: 252 AAGFFAVCIGSPIDVVKSRMMGDST-------YRNTVDCFIKTMKTEGIMAFYKGFLPNF 298

Query: 306 SRQGPFTVVLFVTLEQVRKVF 322
           +R G +  ++F+TLEQV+KVF
Sbjct: 312 TRLGTWNAIMFLTLEQVKKVF 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJY51.2e-13578.40Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2... [more]
Q9SB523.5e-12472.22Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2... [more]
Q9FY686.9e-10462.83Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2... [more]
Q54PY79.1e-6443.89Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium di... [more]
Q9CR622.2e-5741.96Mitochondrial 2-oxoglutarate/malate carrier protein OS=Mus musculus OX=10090 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7T2E38.0e-17298.46Mitochondrial uncoupling protein 5-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B1F28.0e-17298.46mitochondrial uncoupling protein 5-like OS=Cucumis melo OX=3656 GN=LOC103484939 ... [more]
A0A0A0KIS84.0e-17197.84Mitochondrial dicarboxylate carrier protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
A0A6J1GDC41.7e-16695.06mitochondrial uncoupling protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IN726.6e-16694.75mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111477... [more]
Match NameE-valueIdentityDescription
XP_008440559.11.7e-17198.46PREDICTED: mitochondrial uncoupling protein 5-like [Cucumis melo] >KAA0036336.1 ... [more]
XP_004143448.18.2e-17197.84mitochondrial uncoupling protein 5 [Cucumis sativus] >KGN48714.1 hypothetical pr... [more]
XP_038883604.12.4e-17097.53mitochondrial uncoupling protein 5-like [Benincasa hispida][more]
KAG6604059.13.2e-16795.68Mitochondrial uncoupling protein 5, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023544374.11.2e-16695.37mitochondrial uncoupling protein 5-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G22500.18.3e-13778.40uncoupling protein 5 [more]
AT4G24570.12.5e-12572.22dicarboxylate carrier 2 [more]
AT5G09470.14.9e-10562.83dicarboxylate carrier 3 [more]
AT5G19760.12.8e-5237.54Mitochondrial substrate carrier family protein [more]
AT5G58970.11.1e-5138.63uncoupling protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002030Mitochondrial carrier UCP-likePRINTSPR00784MTUNCOUPLINGcoord: 115..127
score: 31.36
coord: 309..324
score: 23.78
coord: 227..245
score: 38.06
coord: 184..201
score: 41.45
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 4..323
e-value: 3.7E-84
score: 284.0
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 4..317
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 4..123
e-value: 9.6E-17
score: 60.7
coord: 130..224
e-value: 5.2E-18
score: 64.8
coord: 233..322
e-value: 7.2E-19
score: 67.5
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 130..221
score: 26.288755
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 4..121
score: 17.557344
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 230..320
score: 26.503286
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 1..324
NoneNo IPR availablePANTHERPTHR45618:SF45MITOCHONDRIAL UNCOUPLING PROTEIN 5coord: 1..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G008040.1Lsi01G008040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071423 malate transmembrane transport
biological_process GO:0006839 mitochondrial transport
biological_process GO:1902356 oxaloacetate(2-) transmembrane transport
biological_process GO:0035435 phosphate ion transmembrane transport
biological_process GO:0071422 succinate transmembrane transport
biological_process GO:1902358 sulfate transmembrane transport
biological_process GO:0015709 thiosulfate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031966 mitochondrial membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0015140 malate transmembrane transporter activity
molecular_function GO:0015131 oxaloacetate transmembrane transporter activity
molecular_function GO:0015141 succinate transmembrane transporter activity
molecular_function GO:0015116 sulfate transmembrane transporter activity
molecular_function GO:0015117 thiosulfate transmembrane transporter activity