Lsi01G003330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G003330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionEpoxide hydrolase-like
Locationchr01: 2916098 .. 2919206 (-)
RNA-Seq ExpressionLsi01G003330
SyntenyLsi01G003330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAATTAACCATTTAATTAAAGAAAAAAATCTTTTGATTGATTTTGTTCTCTTAAAGAGTAAATGATAAATAATTATCCGCTGAGAATTCGACCACCACAAATTCTTTACCAAGAATGTTCACCTCAATCTTTGCAAAATTATTCATAATTTTATCGAAATAGAGAATGAAATGAGTGGCAGGAAGGGAAAGGGATGGACAAATCATTCTCATTCCCCCTTGACATCTCTAAAACAAACCCCATAGTTATTGGTTATTTTTTATAATTTAATTATATGTGTTTTTAATGGGTAATTTTTTGTATTATTAATTTAAATTGTTTGAAATGTTATGGGCTAAACTTAAGAAAATATTTATAGGCTAATATTATGCAGAAGATTTCTTTATTATTATAATCGTTACAATTCCTTTCAACTTTAGCGAACAACTTTCGTCATTTTTTAAAGTTAAATTACAGGGGAAAAAATGTAGTCCTAGTTTTTGCAATTTTGTATTTAACAGTAATAAACTTCCATAAAAATACTAAAATGATGACAATAAATAGAACACTGAAAAGTCTCCGTTCGGTTTTTTCGGAACACCGCCAACAAAGTCAACCCGCCCACAAACATCGACTTTATTTATTTATTTAATCATTCACTGAGCGGCAGTTGGTTCGAAAATTGAAGCAGAGCAGAGTTCAAAATATCTTCGCCGCTCGCTCACTCATTTCCCTCAAATGGATCGAATTCAGCACAATTTCATCGAAGTTGGAGGCCTAAAGCTCCATGTAGCCGAGATCGGAACTGGTGATCTCTCATCTCATCGTCAATTTCTTCACCGGAGCTTGTCTTGCTTACAAATACCTACTCTCTGTCTGTTTGTGCTGCTCCTTTTCCCATTTTTCTTTCAGGTTCCAATGTCGTTGTCTTCTTACACGGATTTCCGGAGATATGGTACTCATGGCGGTACCAGATGATTGCTCTTGCTGACGCCGGCTTCCGAGTACTCGCTCCCGATTACAGAGGATATGGACTCTCCGATTCCCCCGCCGAGCCCTCCAAGGCTTCATTCAACGACCTCATCAGTGATCTCCTTGGGATTCTTGACGCTCTCAACATCCCGAAGGTACTATTCTCCTTAGCATGCGCTTTGATTTGGTTGTAATCGTATTGAAGTTAATAGGGTTCCACTGAAGATTGCATTACATTGACTGCGAGGTGTTTGAAAAAATGAACTGCTGGATGAATTGAGTAATAGGGAAAAAGTGTAAAACATATTGATGTTCATTGTATCAAACCAGGCATATTAACCTTTGATTTTATGATTTTAAGTTAACGGCATTCCAACATAGTTAGTCGCATTGATATATATGGCAAACTAAGGAATCTTCAAACATTACTTTGTATAGGTGTTTGTTGTTGCGAAAGATTTTGGAGCATGGCCAGCTTATTACTTTGCATTGAAACATCCTGAAAGGGCATTGGGAATTGTCACATTGGGAGTGCCATTCCTTCCTCCTGAATCTGTTAAGAGTTCCCAAAGCAACATCCCTGAAGGCGTGTATACTTTAAGATGGCGGGTATGCACATGCTCTACCATTGCCTTACACTCTCCCCTTTCAAAAGGAAACTGAATGGAAGTATTCCAAGTGTGTATTATTTCAATGCTTTGGACACGAATGTATTGTTTGTTGAAAGAAACAATTAGTTTCAAGTTGTTTTTGTTAGAGTGTTGATCTATTATTAAATTTACCTTTTACCTATTAGCTTAAGCTTTTGGGTTAATCAATGATTTAACATAGTATTACAGTAGGTGCTCCTGAGTTTAAAACCTTGCCGTGTCTTATTTTCTCTCCAATTAATATTGATTTCCACTTGTTGGGTCTTCTAATTAAAAGTGAGGGGAGTGTTAGAGTGTTGATATAATATTAAATTTAACTTCTTCTATTTTGGGTCAATTGGTGATTTAATAGTTTTTACTTTTTTAACAAATGGACATTCCTACTATGAGTCTGTGACTATTGATTTTACAGTATTGATTCTGGATAGAGATTTCTATCTCAATAGTTAATAAGCAAATCATAGTTCTTCTACAGAATAAATGAATCTTTTATCATGTTAGGTTGGATTTCAAGTTTCTTAACTTTGTTTGCCTGTTTGTTCTTTTCTTTTACTAACTTGTTGCTTCAACTTTCTCTCTACTCAAGGAACCCGGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTGTTGTCAGGAATGTTTATATCCTCTTCTCCAAAAGTGAAATACCAATAGCTCAAGAAAATCAAGAAGTAATGGACTTAGTAGACCCATCCACCCCTCTTCCTCCTTGGTTCACTGAGGAAGATCTAGCAACTTATGGAGCTCTCTATGAGAAATCTGGATTTGATACTGCATTGAAAATTCCTTACAGGTATGGCACTTGAGTTATGAGATGATCACATCTTGTTTTACTTGGATAATTCTGTTACATTTGATTATGATGGATTGATTCACATGCATGATAGGTCATTCAATGAAGATTTGGGAATAAAGGATCCAAAAGTTGAAATTCCAGCACTCTTTATAATGGGTGAAAAGGACTATGTTCTCAAATTCCCTGAAATTGAGGAATACGTAAGAAGCGAAAGAGTTAAAGATTTTGTTCCCAAATTGGAGATTATCTTTTTACCAGAAGGATCTCATTTTGTTCAGGAGCAATCTCCCGAAGAAGTTAATCAGCTACTACTTGCTTTCCTTGCCAAGCATATTTGACGGTGAACACATTATCAACACTCCAAGTTTTAAATCTTAATAAAACTCAATATGACTCTTTCTGTGTACTTTGTTCTTAAATTCAATCAATAGTTACAATAGGTAGGTTATTATTATTATTATTATTATTATTATTATTATTATTTTGAGTTAAACATGGTGGAGATTCCAATTAACATCAAACCTTTTACTTAAGAGTACACCGTTTATGTTTACTTTGACACGATATATATCTTATTTGGGGAGCTAGACGTTTGTAAACTTTATAGTTGTCTACTTGAAATGTCAGAGGGACTTTCGTCCAAACTAAAAGGATTTATGTATAAAATGTTATGTGAGATC

mRNA sequence

AGAGAATTAACCATTTAATTAAAGAAAAAAATCTTTTGATTGATTTTGTTCTCTTAAAGAGTAAATGATAAATAATTATCCGCTGAGAATTCGACCACCACAAATTCTTTACCAAGAATGTTCACCTCAATCTTTGCAAAATTATTCATAATTTTATCGAAATAGAGAATGAAATGAGTGGCAGGAAGGGAAAGGGATGGACAAATCATTCTCATTCCCCCTTGACATCTCTAAAACAAACCCCATAGTTATTGGTTATTTTTTATAATTTAATTATATGTGTTTTTAATGGGTAATTTTTTGTATTATTAATTTAAATTGTTTGAAATGTTATGGGCTAAACTTAAGAAAATATTTATAGGCTAATATTATGCAGAAGATTTCTTTATTATTATAATCGTTACAATTCCTTTCAACTTTAGCGAACAACTTTCGTCATTTTTTAAAGTTAAATTACAGGGGAAAAAATGTAGTCCTAGTTTTTGCAATTTTGTATTTAACAGTAATAAACTTCCATAAAAATACTAAAATGATGACAATAAATAGAACACTGAAAAGTCTCCGTTCGGTTTTTTCGGAACACCGCCAACAAAGTCAACCCGCCCACAAACATCGACTTTATTTATTTATTTAATCATTCACTGAGCGGCAGTTGGTTCGAAAATTGAAGCAGAGCAGAGTTCAAAATATCTTCGCCGCTCGCTCACTCATTTCCCTCAAATGGATCGAATTCAGCACAATTTCATCGAAGTTGGAGGCCTAAAGCTCCATGTAGCCGAGATCGGAACTGGTTCCAATGTCGTTGTCTTCTTACACGGATTTCCGGAGATATGGTACTCATGGCGGTACCAGATGATTGCTCTTGCTGACGCCGGCTTCCGAGTACTCGCTCCCGATTACAGAGGATATGGACTCTCCGATTCCCCCGCCGAGCCCTCCAAGGCTTCATTCAACGACCTCATCAGTGATCTCCTTGGGATTCTTGACGCTCTCAACATCCCGAAGGTGTTTGTTGTTGCGAAAGATTTTGGAGCATGGCCAGCTTATTACTTTGCATTGAAACATCCTGAAAGGGCATTGGGAATTGTCACATTGGGAGTGCCATTCCTTCCTCCTGAATCTGTTAAGAGTTCCCAAAGCAACATCCCTGAAGGCGTGTATACTTTAAGATGGCGGGAACCCGGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTGTTGTCAGGAATGTTTATATCCTCTTCTCCAAAAGTGAAATACCAATAGCTCAAGAAAATCAAGAAGTAATGGACTTAGTAGACCCATCCACCCCTCTTCCTCCTTGGTTCACTGAGGAAGATCTAGCAACTTATGGAGCTCTCTATGAGAAATCTGGATTTGATACTGCATTGAAAATTCCTTACAGGTCATTCAATGAAGATTTGGGAATAAAGGATCCAAAAGTTGAAATTCCAGCACTCTTTATAATGGGTGAAAAGGACTATGTTCTCAAATTCCCTGAAATTGAGGAATACGTAAGAAGCGAAAGAGTTAAAGATTTTGTTCCCAAATTGGAGATTATCTTTTTACCAGAAGGATCTCATTTTGTTCAGGAGCAATCTCCCGAAGAAGTTAATCAGCTACTACTTGCTTTCCTTGCCAAGCATATTTGACGGTGAACACATTATCAACACTCCAAGTTTTAAATCTTAATAAAACTCAATATGACTCTTTCTGTGTACTTTGTTCTTAAATTCAATCAATAGTTACAATAGGTAGGTTATTATTATTATTATTATTATTATTATTATTATTATTTTGAGTTAAACATGGTGGAGATTCCAATTAACATCAAACCTTTTACTTAAGAGTACACCGTTTATGTTTACTTTGACACGATATATATCTTATTTGGGGAGCTAGACGTTTGTAAACTTTATAGTTGTCTACTTGAAATGTCAGAGGGACTTTCGTCCAAACTAAAAGGATTTATGTATAAAATGTTATGTGAGATC

Coding sequence (CDS)

ATGGATCGAATTCAGCACAATTTCATCGAAGTTGGAGGCCTAAAGCTCCATGTAGCCGAGATCGGAACTGGTTCCAATGTCGTTGTCTTCTTACACGGATTTCCGGAGATATGGTACTCATGGCGGTACCAGATGATTGCTCTTGCTGACGCCGGCTTCCGAGTACTCGCTCCCGATTACAGAGGATATGGACTCTCCGATTCCCCCGCCGAGCCCTCCAAGGCTTCATTCAACGACCTCATCAGTGATCTCCTTGGGATTCTTGACGCTCTCAACATCCCGAAGGTGTTTGTTGTTGCGAAAGATTTTGGAGCATGGCCAGCTTATTACTTTGCATTGAAACATCCTGAAAGGGCATTGGGAATTGTCACATTGGGAGTGCCATTCCTTCCTCCTGAATCTGTTAAGAGTTCCCAAAGCAACATCCCTGAAGGCGTGTATACTTTAAGATGGCGGGAACCCGGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTGTTGTCAGGAATGTTTATATCCTCTTCTCCAAAAGTGAAATACCAATAGCTCAAGAAAATCAAGAAGTAATGGACTTAGTAGACCCATCCACCCCTCTTCCTCCTTGGTTCACTGAGGAAGATCTAGCAACTTATGGAGCTCTCTATGAGAAATCTGGATTTGATACTGCATTGAAAATTCCTTACAGGTCATTCAATGAAGATTTGGGAATAAAGGATCCAAAAGTTGAAATTCCAGCACTCTTTATAATGGGTGAAAAGGACTATGTTCTCAAATTCCCTGAAATTGAGGAATACGTAAGAAGCGAAAGAGTTAAAGATTTTGTTCCCAAATTGGAGATTATCTTTTTACCAGAAGGATCTCATTTTGTTCAGGAGCAATCTCCCGAAGAAGTTAATCAGCTACTACTTGCTTTCCTTGCCAAGCATATTTGA

Protein sequence

MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEVNQLLLAFLAKHI
Homology
BLAST of Lsi01G003330 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 6.3e-38
Identity = 107/321 (33.33%), Postives = 166/321 (51.71%), Query Frame = 0

Query: 4   IQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ V   ++LH  E+G+G   V   HGFPE WYSWRYQ+ ALA AG+RVLA D +G
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSGP-AVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKG 296

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S +P E  +     L  +++  LD L + +   +  D+G    +Y AL +PER   +
Sbjct: 297 YGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAV 356

Query: 124 VTLGVPFLPPESVKSSQSNI---PEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  PF+P     S   +I   P   Y L ++EPG AEA+      + + R    LF  
Sbjct: 357 ASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAEL----EQNLSRTFKSLFRA 416

Query: 184 SEIPIAQENQEV----MDLVDPSTP-LPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 243
           S+  +   ++      + +  P  P L    TEE++  Y   ++KSGF   L   YR+  
Sbjct: 417 SDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNW-YRNME 476

Query: 244 -------EDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPE 303
                  + LG    K+ IPAL +  EKD+VL  P++     S+ ++D++P L+   + +
Sbjct: 477 RNWKWACKSLG---RKILIPALMVTAEKDFVL-VPQM-----SQHMEDWIPHLKRGHIED 536

Query: 304 GSHFVQEQSPEEVNQLLLAFL 309
             H+ Q   P EVNQ+L+ +L
Sbjct: 537 CGHWTQMDKPTEVNQILIKWL 542

BLAST of Lsi01G003330 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.3e-38
Identity = 107/322 (33.23%), Postives = 167/322 (51.86%), Query Frame = 0

Query: 4   IQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ V  G++LH  E+G+G   +   HGFPE W+SWRYQ+ ALA AGFRVLA D +G
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGP-AICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKG 294

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S SP E  + +   L  +++  L+ L IP+   +  D+     +  AL HPER   +
Sbjct: 295 YGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAV 354

Query: 124 VTLGVPFLPPESVKSSQS---NIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  P +PP    S      +IP   Y L ++EPG AEA+      K + R     F  
Sbjct: 355 ASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAEL----EKNMSRTFKSFFRT 414

Query: 184 S-EIPIAQENQEV-MDLVDPSTPLPPWF----TEEDLATYGALYEKSGFDTAL---KIPY 243
           S ++ +   N+   M  +   TP  P      TEE++  Y   ++KSGF   L   +   
Sbjct: 415 SDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTE 474

Query: 244 RSFNEDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSH 303
           R++         K+ +PAL +  EKD VL+ PE+     S+ +++++P L+   + +  H
Sbjct: 475 RNWKWSCKALGRKILVPALMVTAEKDIVLR-PEM-----SKNMENWIPFLKRGHIEDCGH 534

Query: 304 FVQEQSPEEVNQLLLAFLAKHI 313
           + Q + P EVNQ+L+ +L   I
Sbjct: 535 WTQIEKPAEVNQILIKWLKTEI 545

BLAST of Lsi01G003330 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 8.3e-38
Identity = 102/319 (31.97%), Postives = 170/319 (53.29%), Query Frame = 0

Query: 4   IQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ V  G++LH  E+G+G   +   HGFPE W+SWRYQ+ ALA AGFRVLA D +G
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGP-ALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKG 294

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S SP E  + +   L  +++  LD L IP+   +  D+     +  AL +PER   +
Sbjct: 295 YGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAV 354

Query: 124 VTLGVPFLPPESVKSSQS---NIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  PF+PP+   S      +IP   Y L ++EPG AEA+  +  ++T  ++ +    +
Sbjct: 355 ASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEKNMSRT-FKSFFRASDE 414

Query: 184 SEIPIAQENQEVMDLV--DPSTP-LPPWFTEEDLATYGALYEKSGFDTAL---KIPYRSF 243
           +      +  E+  ++   P  P L    TEE++  Y   ++K+GF   L   +   R++
Sbjct: 415 TGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNW 474

Query: 244 NEDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQ 303
                    K+ +PAL +  EKD VL+ PE+     S+ ++ ++P L+   + +  H+ Q
Sbjct: 475 KWSCKGLGRKILVPALMVTAEKDIVLR-PEM-----SKNMEKWIPFLKRGHIEDCGHWTQ 534

Query: 304 EQSPEEVNQLLLAFLAKHI 313
            + P EVNQ+L+ +L   +
Sbjct: 535 IEKPTEVNQILIKWLQTEV 545

BLAST of Lsi01G003330 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.0e-37
Identity = 105/316 (33.23%), Postives = 161/316 (50.95%), Query Frame = 0

Query: 9   IEVGGLKLHVAEIG-TGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRGYGLSD 68
           ++  G++L V E G  G+ VV+  HGFPE+ YSWR+Q+ ALADAG+ VLAPD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  SPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGIVTLGV 128
            P        + L +DL+G+LD +   +   V  D+GA   +   L H +R   +  L V
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 PFLPPESVKSSQ---SNIPEG-VYTLRWREPGRAEADFGRFDAKTVVRNVYIL-----FS 188
           P LP   V  +Q   S   E   Y L ++EPG A+A+     A+T+ R +  L      S
Sbjct: 129 PALPRAQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQS 188

Query: 189 KSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNED-- 248
            +   +A      +D +     LP W ++E+L  Y   + ++GF   L   YR+F+ +  
Sbjct: 189 AAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNW-YRNFDRNWE 248

Query: 249 --LGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFV--PKLEIIFLPEGSHFV 308
               +    + +P+LFI G  D VL F       R++R  + +  P  E++ +    H++
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTF------TRTDRAAEVISGPYREVL-IDGAGHWL 308

BLAST of Lsi01G003330 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.9e-34
Identity = 102/315 (32.38%), Postives = 164/315 (52.06%), Query Frame = 0

Query: 4   IQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ +  G++LH  E+G+G   V   HGFPE W+SWRYQ+ ALA AGFRVLA D +G
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSGP-AVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKG 296

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S +P E  + S   L  D++  L+ L + +   +  D+G    +  AL +PER   +
Sbjct: 297 YGESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAV 356

Query: 124 VTLGVPFLPPESVKSSQSNI---PEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  PF+P     S    I   P   Y L ++EPG AEA+  + +     +N +    +
Sbjct: 357 ASLNTPFMPSNPNVSPMEIIKANPVFDYQLYFQEPGVAEAELEQ-NLDRTFKNFFRAHDE 416

Query: 184 SEIPIAQENQ-EVMDLVDPSTP-LPPWFTEEDLATYGALYEKSGFDTALKIPYRSF--NE 243
           + +   +  +   + +  P  P L    TEED+  Y   ++KSGF   L   YR+   N 
Sbjct: 417 TFLTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNW-YRNMERNW 476

Query: 244 DLGIKDP--KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQ 303
             G K    K+ IPAL +  E D VL  P++     S+ +++++P L+   + +  H+ Q
Sbjct: 477 QWGCKGSGRKILIPALMVTAENDLVL-HPKM-----SKHMENWIPHLKRGHIKDCGHWTQ 536

Query: 304 EQSPEEVNQLLLAFL 309
              P E+N++L+ +L
Sbjct: 537 IDKPAELNRILIEWL 542

BLAST of Lsi01G003330 vs. ExPASy TrEMBL
Match: A0A0A0KHK9 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517180 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.5e-172
Identity = 296/312 (94.87%), Postives = 302/312 (96.79%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFIEVG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIEVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLV+PSTPLPPWFTEEDLATYG LYEKSGFDTALK+PYRSFNED GIK
Sbjct: 181 EIPTAQENQEVMDLVEPSTPLPPWFTEEDLATYGTLYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALFIMGEKDYV KFPEIEEYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 241 DPKVEIPALFIMGEKDYVFKFPEIEEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAKHI
Sbjct: 301 NHLLLTFLAKHI 312

BLAST of Lsi01G003330 vs. ExPASy TrEMBL
Match: A0A1S3AZS9 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103484538 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 2.9e-171
Identity = 293/312 (93.91%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFI+VG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFNED GIK
Sbjct: 181 EIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 241 DPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAK+I
Sbjct: 301 NHLLLTFLAKYI 312

BLAST of Lsi01G003330 vs. ExPASy TrEMBL
Match: A0A5A7U9V8 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003160 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.7e-169
Identity = 293/318 (92.14%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTG------SNVVVFLHGFPEIWYSWRYQMIALADAGFR 60
           MDRI HNFI+VG LKLHVAEIGTG      SNVVVFLHGFPEIWYSWRYQMIALADAGFR
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGNHSSHRSNVVVFLHGFPEIWYSWRYQMIALADAGFR 60

Query: 61  VLAPDYRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120
           VLAPDYRGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK
Sbjct: 61  VLAPDYRGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120

Query: 121 HPERALGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180
           HPERALGIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY
Sbjct: 121 HPERALGIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180

Query: 181 ILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 240
           ILFSKSEIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFN
Sbjct: 181 ILFSKSEIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFN 240

Query: 241 EDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQE 300
           ED GIKDP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQE
Sbjct: 241 EDWGIKDPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQE 300

Query: 301 QSPEEVNQLLLAFLAKHI 313
           QSPEEVN LLL FLAK+I
Sbjct: 301 QSPEEVNHLLLTFLAKYI 318

BLAST of Lsi01G003330 vs. ExPASy TrEMBL
Match: A0A5D3CMT0 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007310 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 8.0e-169
Identity = 292/318 (91.82%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTG------SNVVVFLHGFPEIWYSWRYQMIALADAGFR 60
           MDRI HNFI+VG LKLHVAEIGTG      SNVVVFLHGFPEIWYSWRYQMIALADAGFR
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGNHSSHRSNVVVFLHGFPEIWYSWRYQMIALADAGFR 60

Query: 61  VLAPDYRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120
           VLAPDYRGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK
Sbjct: 61  VLAPDYRGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120

Query: 121 HPERALGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180
           HPERALGIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY
Sbjct: 121 HPERALGIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180

Query: 181 ILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 240
           ILFSKSEIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFN
Sbjct: 181 ILFSKSEIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFN 240

Query: 241 EDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQE 300
           ED GIKDP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQE
Sbjct: 241 EDWGIKDPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQE 300

Query: 301 QSPEEVNQLLLAFLAKHI 313
           QSP+EVN LLL FLAK+I
Sbjct: 301 QSPKEVNHLLLTFLAKYI 318

BLAST of Lsi01G003330 vs. ExPASy TrEMBL
Match: A0A6J1GFK2 (uncharacterized protein LOC111453471 OS=Cucurbita moschata OX=3662 GN=LOC111453471 PE=4 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.2e-167
Identity = 283/311 (91.00%), Postives = 302/311 (97.11%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MD+IQHNFIEV GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIA+A+AGFR +APDY
Sbjct: 1   MDQIQHNFIEVAGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIAIANAGFRAIAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKV +V KDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVIIVGKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           G+VTLGVPF+PP SV+S+QS+IPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GVVTLGVPFMPPGSVRSTQSHIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           E+PIAQENQE+MDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFNED G+K
Sbjct: 181 EMPIAQENQEIMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGVK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALFI+GEKDYVLKFP+IEEYVRS RVK+FVPKLEII+LPEGSHFVQEQSPEEV
Sbjct: 241 DPKVEIPALFIVGEKDYVLKFPDIEEYVRSGRVKEFVPKLEIIYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKH 312
           NQLLL FLA H
Sbjct: 301 NQLLLTFLANH 311

BLAST of Lsi01G003330 vs. NCBI nr
Match: XP_038881162.1 (bifunctional epoxide hydrolase 2-like [Benincasa hispida])

HSP 1 Score: 615.5 bits (1586), Expect = 2.5e-172
Identity = 298/312 (95.51%), Postives = 304/312 (97.44%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFIEVGGL LHVAEIGTGSN+VVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIEVGGLTLHVAEIGTGSNIVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPESVKS QS IPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESVKSVQSIIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRS NED GIK
Sbjct: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSLNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           +PKVEIPALFIMGEKDYV KFPEIEEYVRSERVKDFVPKLEII++PEGSHFVQEQSPEEV
Sbjct: 241 EPKVEIPALFIMGEKDYVFKFPEIEEYVRSERVKDFVPKLEIIYIPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           NQLLL FLAKHI
Sbjct: 301 NQLLLTFLAKHI 312

BLAST of Lsi01G003330 vs. NCBI nr
Match: XP_004134720.1 (uncharacterized protein LOC101208531 [Cucumis sativus] >KGN49210.1 hypothetical protein Csa_004043 [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 7.2e-172
Identity = 296/312 (94.87%), Postives = 302/312 (96.79%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFIEVG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIEVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLV+PSTPLPPWFTEEDLATYG LYEKSGFDTALK+PYRSFNED GIK
Sbjct: 181 EIPTAQENQEVMDLVEPSTPLPPWFTEEDLATYGTLYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALFIMGEKDYV KFPEIEEYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 241 DPKVEIPALFIMGEKDYVFKFPEIEEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAKHI
Sbjct: 301 NHLLLTFLAKHI 312

BLAST of Lsi01G003330 vs. NCBI nr
Match: XP_008439884.2 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 610.9 bits (1574), Expect = 6.1e-171
Identity = 293/312 (93.91%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFI+VG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFNED GIK
Sbjct: 181 EIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 241 DPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAK+I
Sbjct: 301 NHLLLTFLAKYI 312

BLAST of Lsi01G003330 vs. NCBI nr
Match: KAA0052703.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 604.4 bits (1557), Expect = 5.7e-169
Identity = 293/318 (92.14%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTG------SNVVVFLHGFPEIWYSWRYQMIALADAGFR 60
           MDRI HNFI+VG LKLHVAEIGTG      SNVVVFLHGFPEIWYSWRYQMIALADAGFR
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGNHSSHRSNVVVFLHGFPEIWYSWRYQMIALADAGFR 60

Query: 61  VLAPDYRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120
           VLAPDYRGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK
Sbjct: 61  VLAPDYRGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120

Query: 121 HPERALGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180
           HPERALGIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY
Sbjct: 121 HPERALGIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180

Query: 181 ILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 240
           ILFSKSEIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFN
Sbjct: 181 ILFSKSEIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFN 240

Query: 241 EDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQE 300
           ED GIKDP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQE
Sbjct: 241 EDWGIKDPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQE 300

Query: 301 QSPEEVNQLLLAFLAKHI 313
           QSPEEVN LLL FLAK+I
Sbjct: 301 QSPEEVNHLLLTFLAKYI 318

BLAST of Lsi01G003330 vs. NCBI nr
Match: TYK13121.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 602.8 bits (1553), Expect = 1.7e-168
Identity = 292/318 (91.82%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTG------SNVVVFLHGFPEIWYSWRYQMIALADAGFR 60
           MDRI HNFI+VG LKLHVAEIGTG      SNVVVFLHGFPEIWYSWRYQMIALADAGFR
Sbjct: 1   MDRIHHNFIQVGALKLHVAEIGTGNHSSHRSNVVVFLHGFPEIWYSWRYQMIALADAGFR 60

Query: 61  VLAPDYRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120
           VLAPDYRGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK
Sbjct: 61  VLAPDYRGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALK 120

Query: 121 HPERALGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180
           HPERALGIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY
Sbjct: 121 HPERALGIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 180

Query: 181 ILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 240
           ILFSKSEIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFN
Sbjct: 181 ILFSKSEIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFN 240

Query: 241 EDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQE 300
           ED GIKDP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQE
Sbjct: 241 EDWGIKDPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQE 300

Query: 301 QSPEEVNQLLLAFLAKHI 313
           QSP+EVN LLL FLAK+I
Sbjct: 301 QSPKEVNHLLLTFLAKYI 318

BLAST of Lsi01G003330 vs. TAIR 10
Match: AT3G51000.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 208.0 bits (528), Expect = 1.1e-53
Identity = 112/316 (35.44%), Postives = 186/316 (58.86%), Query Frame = 0

Query: 4   IQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           ++   I+  G+ L+VAE G     +V+ LHGFPE WYSWR+Q+  L+  G+ V+APD RG
Sbjct: 5   VREKKIKTNGIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRG 64

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG SDS       + + L++D++G+LD     + FV   D+GA   +   L  P+R  G 
Sbjct: 65  YGDSDSLPSHESYTVSHLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGF 124

Query: 124 VTLGVPFLPPE-SVKSSQ--SNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
           ++L VP+ P +  +K S       +G+Y  ++++PGRAEA F + D  +V++  ++L ++
Sbjct: 125 ISLSVPYFPRDPKLKPSDFFKIFGDGLYITQFQKPGRAEAAFAKHDCLSVMKK-FLLITR 184

Query: 184 SEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGI 243
           ++  +A  + E++D ++  + +P W TEE++  Y   +++SGF   L   YRS + +  I
Sbjct: 185 TDYLVAPPDTEIIDHLEIPSTIPDWITEEEIQVYAEKFQRSGFTGPLNY-YRSMDMNWEI 244

Query: 244 ----KDPKVEIPALFIMGEKDYVLKFPE-IEEYVRSERVKDFVPKLEIIFLPEGSHFVQE 303
               +D K+ +P  FI G+KD   + P    EYV+ E  K  VP LEI+ +  G HF+Q+
Sbjct: 245 LAPWQDSKIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGGHHFIQQ 304

Query: 304 QSPEEVNQLLLAFLAK 311
           +  E+V+Q +L+FL K
Sbjct: 305 EKSEQVSQEILSFLNK 318

BLAST of Lsi01G003330 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 206.5 bits (524), Expect = 3.2e-53
Identity = 106/319 (33.23%), Postives = 176/319 (55.17%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           M++I+H  I   G+ +HVA IG+G  V++F+HGFP++WYSWR+Q+++ A  G+R +APD 
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGP-VILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDL 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYG SD+P      +   ++ DL+G+LD+L + +VF+V  D+GA  A++  +  P+R  
Sbjct: 61  RGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVN 120

Query: 121 GIVTLGVPFLP----PESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYIL 180
            +V   V F P     + V + ++   +  Y  R++EPG  E DF + D K ++   +  
Sbjct: 121 ALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITR-FFT 180

Query: 181 FSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNED 240
                 P   ++     L DP + LP W TE+D+  YG  + + GF   L   YR+ N  
Sbjct: 181 SRNPRPPCIPKSVGFRGLPDPPS-LPAWLTEQDVRFYGDKFSQKGFTGGLNY-YRALNLS 240

Query: 241 LGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG-SHF 300
             +  P    ++++P  FI+G+ D     P  +EY+    +K  VP L+ + + EG  HF
Sbjct: 241 WELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHF 300

Query: 301 VQEQSPEEVNQLLLAFLAK 311
           + ++ P+EV   +  F  K
Sbjct: 301 LHQEKPDEVTDHIYGFFKK 315

BLAST of Lsi01G003330 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 198.7 bits (504), Expect = 6.7e-51
Identity = 109/322 (33.85%), Postives = 180/322 (55.90%), Query Frame = 0

Query: 4   IQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           ++H  +   G+ +HVA  G     +V+ LHGFPE+WYSWR+Q+  LA  G+R +APD RG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNI---PKVFVVAKDFGAWPAYYFALKHPERA 123
           YG SD+PAE S  +  +++ DL+ ++ AL      KVFVV  D+GA  A+Y  L  P+R 
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 LGIVTLGVPFL------PPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNV 183
             +V L VPF         + V   ++   +  Y  R++E G  EA+      + V++ +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 YILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSF 243
               +   + I  +++        + PLP W TEED+A + + +E+ GF   +   YR+F
Sbjct: 181 LTYRTPGPV-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNY-YRNF 240

Query: 244 NEDLGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG- 303
           N +  +  P    K+++P  F++GE D V   P ++EY+   + K+ VP LE   + EG 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 304 SHFVQEQSPEEVNQLLLAFLAK 311
           +HF+ ++ P+E+ Q++L F++K
Sbjct: 301 AHFINQEKPQEILQIILDFISK 320

BLAST of Lsi01G003330 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 5.3e-48
Identity = 105/320 (32.81%), Postives = 179/320 (55.94%), Query Frame = 0

Query: 4   IQHNFIEVGGLKLHVAEIG-TGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           ++H  +   G+ +HVA  G +   +V+ LHGFPE+WYSWR+Q+  LA  G+R +APD RG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDAL--NIPKVFVVAKDFGAWPAYYFALKHPERAL 123
           YG SD+PAE S  +  +++ DL+ ++  L     KVFVV  D+GA  A+Y  L  P++  
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 GIVTLGVP--FLPPE----SVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 183
            +V L VP  F P +     V   ++      Y  R++E G  EA+      + V++ + 
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 ILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 243
              +   + I  +++        + PLP W TEED+A + + +++ GF   +   YR+FN
Sbjct: 181 TYRTPGPL-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNY-YRNFN 240

Query: 244 EDLGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG-S 303
            +  +  P    K+++P  F++GE D V   P ++EY+   + K+ VP +E   + EG +
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 304 HFVQEQSPEEVNQLLLAFLA 310
           HF+ ++ P+E+ Q++L F++
Sbjct: 301 HFLNQEKPQEILQIILDFIS 318

BLAST of Lsi01G003330 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-47
Identity = 106/325 (32.62%), Postives = 180/325 (55.38%), Query Frame = 0

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPD 60
           M+ I H  + V G+ +H+AE G     VV+ LHGFP++WY+WR+Q+  L+  G+R +APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  YRGYGLSDSPAEPSKASFNDLISDLLGILDAL--NIPKVFVVAKDFGAWPAYYFALKHPE 120
            RGYG SDSP   S+ +  +++ DL+ +LD++  N  KVF+V  D+GA   ++  L  PE
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RALGIVTLGVPFL----PPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNV 180
           +  G V L VP+       + V+  ++   +  Y  R++EPG+ E +    D +  +RN+
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 YILFSKSEIPIAQENQEVMDLVDPST---PLPPWFTEEDLATYGALYEKSGFDTALKIPY 240
           +   +    PI  ++    +  +P++    LP WF+++DL  Y + +EK+GF   L   Y
Sbjct: 181 FTGRTLGP-PILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNY-Y 240

Query: 241 RSFNEDLGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKL-EIIFL 300
           R+ + +  +  P    K+++P  F+ G+ D V   P ++EY+        VP L EI+ +
Sbjct: 241 RAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVI 300

Query: 301 PEGSHFVQEQSPEEVNQLLLAFLAK 311
            +  HFV ++ P+EV   +  F  K
Sbjct: 301 EDAGHFVNQEKPQEVTAHINDFFTK 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P349136.3e-3833.33Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P802996.3e-3833.23Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349148.3e-3831.97Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
I6YGS07.0e-3733.23Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C21.9e-3432.38Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KHK93.5e-17294.87AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517... [more]
A0A1S3AZS92.9e-17193.91bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103484538 PE... [more]
A0A5A7U9V82.7e-16992.14Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5D3CMT08.0e-16991.82Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1GFK21.2e-16791.00uncharacterized protein LOC111453471 OS=Cucurbita moschata OX=3662 GN=LOC1114534... [more]
Match NameE-valueIdentityDescription
XP_038881162.12.5e-17295.51bifunctional epoxide hydrolase 2-like [Benincasa hispida][more]
XP_004134720.17.2e-17294.87uncharacterized protein LOC101208531 [Cucumis sativus] >KGN49210.1 hypothetical ... [more]
XP_008439884.26.1e-17193.91PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
KAA0052703.15.7e-16992.14bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa][more]
TYK13121.11.7e-16891.82bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G51000.11.1e-5335.44alpha/beta-Hydrolases superfamily protein [more]
AT4G02340.13.2e-5333.23alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.16.7e-5133.85soluble epoxide hydrolase [more]
AT2G26750.15.3e-4832.81alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.11.2e-4732.62alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 98..111
score: 29.87
coord: 112..125
score: 28.9
coord: 285..307
score: 29.84
coord: 32..50
score: 55.5
coord: 52..67
score: 56.53
coord: 246..262
score: 33.42
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 52..67
score: 47.2
coord: 112..125
score: 30.3
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 26..284
e-value: 7.3E-22
score: 78.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..311
e-value: 2.8E-86
score: 292.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..310
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 3..311
NoneNo IPR availablePANTHERPTHR43329:SF106BIFUNCTIONAL EPOXIDE HYDROLASE 2-LIKE ISOFORM X1coord: 3..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G003330.1Lsi01G003330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity