Lcy13g013850 (gene) Sponge gourd (P93075) v1

BLAST of Lcy13g013850 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.8e-63
Identity = 193/596 (32.38%), Postives = 315/596 (52.85%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNM--ELQQELVKIF 66
           +VTTRS  VA ++     H+L  LS   CWS+F K+   N     +    +L + +V   
Sbjct: 311 LVTTRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKC 370

Query: 67  GGLPLVARVLGRAVKFEGDYVRWVKILES-VVKTPLQENFVSSILKLSVDHLPSSSLKQC 126
            GLPL  + LG  ++FEG  + W ++L S +   P  ++ +  +L++S  +LP + LK+C
Sbjct: 371 RGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLP-AHLKRC 430

Query: 127 FAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDV 186
           FAY S FPK   F+K++++  WMA+GF+Q   +   +K +EE+G  YF  L SR L Q  
Sbjct: 431 FAYCSIFPKGHAFEKDKVVLLWMAEGFLQ---QTRSSKNLEELGNEYFSELESRSLLQKT 490

Query: 187 VKDDRGRIIYCKMHDLIHDIACGVSND--------QKLQLDPSSLWDKELCINGSEKFR- 246
               + R I   MHD I+++A   S +         KLQ+   + +   L  N +E    
Sbjct: 491 ----KTRYI---MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEF 550

Query: 247 SKLRTVVFIEKMLHKKEIN---------VIHDKIL-NLICLRVLLMNSQSIYKLPNSIGK 306
             LR V F+   L     N         ++ +K+L  L  LRVL ++   I +LP    K
Sbjct: 551 EALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFK 610

Query: 307 -LKHLRYLDISGCPIEKLPESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDF 366
            + H R+LD+S   +EKLP+S+  +YNLQTLL    + + +LP ++ + +NLR+    D 
Sbjct: 611 NISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYL---DL 670

Query: 367 GFEETKQMPPNMGQLTQLQTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEE 426
              + +QMP   G+L  LQTL+ F      G +++ELG L +L G L +++L++V+   +
Sbjct: 671 IGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVAD 730

Query: 427 AKTAKLLEKENLCELTFE-RSLLSGTED-----KTHNYLEVLEGLEPHKNLQFLNIKHFP 486
           A  A L  K++L E+ F  R+  S +E+     +T N  EV E L PH++++ L I+ + 
Sbjct: 731 AAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYK 790

Query: 487 NKQLPHNIFVES---LVAIHLHECGIC-ELSMLGQLTNLEELHNSFLYHLQNLSIQDFGR 546
            ++ P  +   S   +V I L EC  C  L  LGQL  L+ELH S +  LQ++  + +  
Sbjct: 791 GRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFS 850

Query: 547 IEALREW----LRNLTSLRTLSL-SFCKNLKLLPSKGAMLHLTRLSRLHVFQCPQL 565
            + LR+      R+L +LR  +L  + + L +  ++G +     L +L + +CP+L
Sbjct: 851 DQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDL--FPSLKKLFILRCPEL 890

BLAST of Lcy13g013850 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 222.2 bits (565), Expect = 1.5e-56
Identity = 181/568 (31.87%), Postives = 281/568 (49.47%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGV-TMFSNMELQQELVKIFG 66
           ++TTR  ++  ++ T   + L  LS + CW +FK+ A  +   T    ME+ +E+VK  G
Sbjct: 288 LITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCG 347

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVV-KTPLQENFVSSILKLSVDHLPSSSLKQCF 126
           G+PL A+ LG  ++F+ +   W  + +S +   P  EN V   L+LS  HLP   L+QCF
Sbjct: 348 GVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLP-LDLRQCF 407

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDV- 186
           AY + FPKD + +KE LI  WMA  F+  +     N  +E++G   ++ L  R  FQ++ 
Sbjct: 408 AYCAVFPKDTKIEKEYLIALWMAHSFLLSKG----NMELEDVGNEVWNELYLRSFFQEIE 467

Query: 187 VKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFI 246
           VK  +    Y KMHDLIHD+A  +          SS   +++ +   E     +     +
Sbjct: 468 VKSGK---TYFKMHDLIHDLATSM-----FSASASSRSIRQINVKDDEDMMFIVTNYKDM 527

Query: 247 EKMLHKKEINVIHDKIL-NLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLP 306
             +   + ++     +    + LRVL +++    +LP+S+G L HLRYLD+SG  I  LP
Sbjct: 528 MSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLP 587

Query: 307 ESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQ 366
           + +  L NLQTL       +  LPK      +LR+             MPP +G LT L+
Sbjct: 588 KRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC---PLTSMPPRIGLLTCLK 647

Query: 367 TLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFER 426
           TL  F+ G  KG +L EL  L NL+G +++  LE+V +  EAK A L  K NL  L+   
Sbjct: 648 TLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSW 707

Query: 427 SLLSGTEDKTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLP---HNIFVESLVAIHLHECG 486
              +  E +    ++VLE L+PH NL++L I  F    LP   ++  ++++V+I +  C 
Sbjct: 708 DRPNRYESEE---VKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCE 767

Query: 487 ICE-LSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALRE----WLRNLTSLRTLSLSF 546
            C  L   G+L  LE L       LQ+ S++    +E + +      R   SLR L +  
Sbjct: 768 NCSCLPPFGELPCLESL------ELQDGSVE----VEYVEDSGFLTRRRFPSLRKLHIGG 825

Query: 547 CKNLKLLPSKGAMLHLTRLSRLHVFQCP 563
             NLK L           L  + +  CP
Sbjct: 828 FCNLKGLQRMKGAEQFPVLEEMKISDCP 825

BLAST of Lcy13g013850 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 217.6 bits (553), Expect = 3.7e-55
Identity = 172/498 (34.54%), Postives = 260/498 (52.21%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSN-MELQQELVKIFG 66
           + TTR  +V  ++ T   + L  LS + CW +F + A  +   +  N M + +E+VK  G
Sbjct: 288 LTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCG 347

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVV-KTPLQENFVSSILKLSVDHLPSSSLKQCF 126
           G+PL A+ LG  ++F+ +   W  + +S +   P  E+ +   L+LS  HLP   L+QCF
Sbjct: 348 GVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLP-LDLRQCF 407

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDVV 186
            Y + FPKD +  KE LI  WMA GF+  +     N  +E++G   ++ L  R  FQ+ +
Sbjct: 408 VYCAVFPKDTKMAKENLIAFWMAHGFLLSKG----NLELEDVGNEVWNELYLRSFFQE-I 467

Query: 187 KDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFIE 246
           + + G+  Y KMHDLIHD+A  +        + SS   +E+  N    +   + ++ F E
Sbjct: 468 EVESGK-TYFKMHDLIHDLATSL-----FSANTSSSNIREINAN----YDGYMMSIGFAE 527

Query: 247 KMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISG-CPIEKLPE 306
            +      +     +   + LRVL + + ++ +LP+SIG L HLRYLD+SG   I  LP+
Sbjct: 528 VV-----SSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPK 587

Query: 307 SIVLLYNLQTL-LKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQ 366
            +  L NLQTL L   D+L   LPK      +LR+          T   PP +G LT L+
Sbjct: 588 RLCKLQNLQTLDLHYCDSLSC-LPKQTSKLGSLRNLLLDGCSLTST---PPRIGLLTCLK 647

Query: 367 TLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFER 426
           +LS F+ G  KG +L EL  L NL G +++ KL++V    +AK A L  K NL  L    
Sbjct: 648 SLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSL---- 707

Query: 427 SLLSGTEDKTHNY-LEVLEGLEPHKNLQFLNIKHFPNKQLP---HNIFVESLVAIHLHEC 486
             LS   D  H Y  EVLE L+PH NL++L I  F   +LP   +   ++++V+I +  C
Sbjct: 708 -CLSWDLDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGC 754

Query: 487 GICE-LSMLGQLTNLEEL 496
             C  L   G+L  LE L
Sbjct: 768 ENCSCLPPFGELPCLESL 754

BLAST of Lcy13g013850 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.6e-53
Identity = 182/574 (31.71%), Postives = 284/574 (49.48%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNM-ELQQELVKIFG 66
           + TTR  +V  ++ T   + L  LS + CW +F + A  +   +  N+  + +E+VK  G
Sbjct: 289 LTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSG 348

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVV-KTPLQENFVSSILKLSVDHLPSSSLKQCF 126
           G+PL A+ LG  + F+ +   W  + +S +   P  E+ +   L+LS   LP   LKQCF
Sbjct: 349 GVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLP-LDLKQCF 408

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDV- 186
           AY + FPKD + +KE+LI  WMA GF+  +     N  +E++G+  +  L  R  FQ++ 
Sbjct: 409 AYCAVFPKDAKMEKEKLISLWMAHGFLLSKG----NMELEDVGDEVWKELYLRSFFQEIE 468

Query: 187 VKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFI 246
           VKD +    Y KMHDLIHD+A  +        + SS   +E+    ++   + + ++ F 
Sbjct: 469 VKDGK---TYFKMHDLIHDLATSL-----FSANTSSSNIREI----NKHSYTHMMSIGFA 528

Query: 247 EKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLPE 306
           E +            +   I LRVL +   +  KLP+SIG L HLRYL++ G  +  LP+
Sbjct: 529 EVVFF-----YTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMRSLPK 588

Query: 307 SIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQT 366
            +  L NLQTL     T +  LPK      +LR+      G +    MPP +G LT L+T
Sbjct: 589 QLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLD--GSQSLTCMPPRIGSLTCLKT 648

Query: 367 LSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFERS 426
           L  F+ G  KG +L ELG L NL G + +  LE+V + ++AK A L  K NL  L+   +
Sbjct: 649 LGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWN 708

Query: 427 LLSGTEDKTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLP---HNIFVESLVAIHLHECGI 486
              G        ++VLE L+PH NL  L I  F    LP   ++  ++++V+I +     
Sbjct: 709 NF-GPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRN 768

Query: 487 CE-LSMLGQLTNLE--ELH--NSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFC 546
           C  L   G L  LE  ELH  ++ + +++ + I     + +         SLR L +   
Sbjct: 769 CSCLPPFGDLPCLESLELHWGSADVEYVEEVDID----VHSGFPTRIRFPSLRKLDIWDF 828

Query: 547 KNLKLLPSKGAMLHLTRLSRLHVFQCPQLRLGQS 570
            +LK L  K        L  + + +CP L L  +
Sbjct: 829 GSLKGLLKKEGEEQFPVLEEMIIHECPFLTLSSN 832

BLAST of Lcy13g013850 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.0e-49
Identity = 173/570 (30.35%), Postives = 275/570 (48.25%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLS-HDQCWSIFKKSANANGVTMFSNMELQQELVKIFG 66
           + TTR  +V  ++ T   +HL  LS HD      +++         + + + +E+VK  G
Sbjct: 290 LATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCG 349

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVV-KTPLQENFVSSILKLSVDHLPSSSLKQCF 126
           G+PL A+ LG  ++F+ +   W  + ++ +   P  E+ +   L+LS  HLP   L+QCF
Sbjct: 350 GVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHLP-LDLRQCF 409

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDVV 186
           AY + FPKD +  KE LI  WMA GF+  +     N  +E++G   ++ L  R  FQ++ 
Sbjct: 410 AYCAVFPKDTKMIKENLITLWMAHGFLLSKG----NLELEDVGNEVWNELYLRSFFQEI- 469

Query: 187 KDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFIE 246
            + +    Y K+HDLIHD+A  + +      +   +  K+     S  F + + +  +  
Sbjct: 470 -EAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVSS--YSP 529

Query: 247 KMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLPES 306
            +L K             + LRVL ++   + +LP+SIG L HLRYLD+S      LPE 
Sbjct: 530 SLLKK------------FVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPER 589

Query: 307 IVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQTL 366
           +  L NLQTL       +  LPK      +LRH          T   PP +G LT L+TL
Sbjct: 590 LCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTST---PPRIGLLTCLKTL 649

Query: 367 SNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFERSL 426
             FI G  KG +L EL  L NL G +++  LE+V +  +A+ A L  K NL  L+     
Sbjct: 650 GFFIVGSKKGYQLGELKNL-NLCGSISITHLERVKNDTDAE-ANLSAKANLQSLS----- 709

Query: 427 LSGTEDKTHNY----LEVLEGLEPHKNLQFLNIKHFPNKQLP---HNIFVESLVAIHLHE 486
           +S   D  + Y    ++VLE L+PH NL++L I  F   + P   ++  +E ++++ +  
Sbjct: 710 MSWDNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKS 769

Query: 487 CGICE-LSMLGQLTNLEELHNSFLYHLQNLSIQ----DFGRIEALREWLRNLTSLRTLSL 546
           C  C  L   G+L  LE L       LQN S +    +   + +     R+  SL+ L +
Sbjct: 770 CKNCLCLPPFGELPCLENL------ELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRI 822

Query: 547 SFCKNLKLLPSKGAMLHLTRLSRLHVFQCP 563
            F ++LK L  +        L  + +  CP
Sbjct: 830 WFFRSLKGLMKEEGEEKFPMLEEMAILYCP 822

BLAST of Lcy13g013850 vs. ExPASy TrEMBL
Match: A0A6J1DXY1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111025175 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 3.9e-164
Identity = 336/582 (57.73%), Postives = 419/582 (71.99%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNMELQQELVKIFGG 66
           +VTTRS EVAK++ET P HHL +LS DQCWS+F +SAN NG+++ S++++ QELVK  GG
Sbjct: 305 LVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVVQELVKKIGG 364

Query: 67  LPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ---ENFVSSILKLSVDHLPSSSLKQC 126
           +PLVARVLGRAVKFEGDY  WV  LES+++T LQ   E+FV SILKLSVD LP SSLKQC
Sbjct: 365 IPLVARVLGRAVKFEGDYETWVTTLESIIRTRLQLQEESFVLSILKLSVDRLPLSSLKQC 424

Query: 127 FAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDV 186
           F+Y SNFPKDF F+KE LI+ WMAQGFIQ +E R+ NKTME+ G+ YF ILLSRCLFQD+
Sbjct: 425 FSYCSNFPKDFVFEKEPLIRMWMAQGFIQLQERRS-NKTMEDEGDTYFKILLSRCLFQDI 484

Query: 187 VKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCING-SEKFRSKLRTVVF 246
           +KD +GRII  KMHDLIHDIAC +SND+KL LDP +L D E   N  SEK  SKLRTV+ 
Sbjct: 485 IKDGKGRIISFKMHDLIHDIACNISNDKKLHLDPCNLLDNEHWRNEISEKVASKLRTVIC 544

Query: 247 IEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLP 306
             + LH++E  VI DK+ N I LRVL++N  +  KLP+SIGKLKHLRYLDISGC +++LP
Sbjct: 545 RRETLHRRE-QVIDDKMSNFIWLRVLIINFWNAKKLPDSIGKLKHLRYLDISGCSMDELP 604

Query: 307 ESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDF-GFEETKQMPPNMGQLTQL 366
           ESIV LY+LQT L+I+ T+IVDLPKNL   V+LRH EFS F G ++  QMP ++ QL QL
Sbjct: 605 ESIVSLYHLQT-LRIEGTMIVDLPKNLGKLVSLRHLEFSFFLGIKKILQMPSHLSQLVQL 664

Query: 367 QTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFE 426
           QTLS FI GF+KG K+ ELGPLKNLKG LNL  LE++ SKEEA  AKL EKENL ELTF 
Sbjct: 665 QTLSTFIVGFEKGRKITELGPLKNLKGSLNLSNLERIKSKEEAMFAKLGEKENLHELTFT 724

Query: 427 RSLLSGTEDKTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECGIC 486
            +  S  E   HN L+VLEGL+PHK+LQ L+I+ F  + LP+ IFVE+LVAI L +C  C
Sbjct: 725 WTCSSEREYNNHNDLDVLEGLQPHKSLQSLSIQSFLGECLPNKIFVENLVAISLVDCENC 784

Query: 487 E-LSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNLKL 546
           E L MLGQLTNLEEL  S L+ ++++  + +G     R +      L+  +L+   NL+ 
Sbjct: 785 ERLPMLGQLTNLEELCISGLHSVRSIGNEFYGNDSDQRTF---FPKLKKFTLNKMSNLE- 844

Query: 547 LPSKGAMLHLTRLSRLHVFQCPQLRLGQSHTERAKLFHLPTI 583
                    +T    L VF C ++      +E  KL  +P I
Sbjct: 845 -----QWEEVTLAPNLTVFPCIEM---WKISECFKLLKVPKI 871

BLAST of Lcy13g013850 vs. ExPASy TrEMBL
Match: A0A6J1FYJ5 (disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 1.1e-153
Identity = 311/558 (55.73%), Postives = 395/558 (70.79%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVE-TFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIF 66
           +VTTR+ EV K++E   P HH+ +LS +QCWS+F +SANANG+   SN+E +++ELVK F
Sbjct: 308 LVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKF 367

Query: 67  GGLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ---ENFVSSILKLSVDHLPSSSLK 126
           GG+PLVARVLG AVKFE DY  WV +LE V+++PL    +NF   ILKLSVD+LP SSLK
Sbjct: 368 GGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDYLP-SSLK 427

Query: 127 QCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQ 186
            CFAY SNFPKD+EF+K+ LIQ W+AQGFIQ +E R+C +T+EE GE YF ILLSRCLFQ
Sbjct: 428 PCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKILLSRCLFQ 487

Query: 187 DVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVV 246
           DVVKD RGRII CKMHDLIHD+AC VSNDQK  LD  +  D+EL  N ++   SKLR + 
Sbjct: 488 DVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLDSCTSSDEELWRNRTKNIGSKLRMIA 547

Query: 247 FIEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKL 306
             E M H+ E   +HDKI + +CLRVL+MNS+SIYKLP SIG+LKHLRY+DISGC I+KL
Sbjct: 548 CHEGMPHRIE-KAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDISGCLIQKL 607

Query: 307 PESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQL 366
           P+SIVLLYNL+T L+I  + I +LPK+LR  VNLRH EFS+  F + K+MPP++ QL  L
Sbjct: 608 PKSIVLLYNLKT-LRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPHLSQLIHL 667

Query: 367 QTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFE 426
           QTLS F+ GF+K C++AELGPLKNLKG L +  LE+V S+EEAK+A  +EKE L EL  E
Sbjct: 668 QTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEKLRELVLE 727

Query: 427 RSLLSGTED--KTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECG 486
               + TED    HN  +VLEGL+PHK L  L+I  F  + L +NIFV++LVAI L +C 
Sbjct: 728 WGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVAISLVDCR 787

Query: 487 IC-ELSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNL 546
            C  L MLG L +LEEL  S L  ++++  + +G          +   LR L +  C  L
Sbjct: 788 NCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGN--------HSNQHLRVLQILECSKL 847

Query: 547 KLLPSKGAMLHLTRLSRL 557
             LP+     H  +  RL
Sbjct: 848 TKLPNGLQFCHSIQHLRL 854

BLAST of Lcy13g013850 vs. ExPASy TrEMBL
Match: A0A6J1FWC6 (disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.8e-153
Identity = 307/566 (54.24%), Postives = 399/566 (70.49%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVE-TFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIF 66
           +VTTR+ EV K++E   P HH+ +LS +QCWS+F +SANANG+   SN+E +++ELVK F
Sbjct: 280 LVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKF 339

Query: 67  GGLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ---ENFVSSILKLSVDHLPSSSLK 126
           GG+PLVARVLG AVKFE DY  WV +LE V+++PL    +NF   ILKLSVD+LP SSLK
Sbjct: 340 GGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDYLP-SSLK 399

Query: 127 QCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQ 186
            CFAY SNFPKD+EF+K+ LIQ W+AQGFIQ +E R+C +T+EE GE YF ILLSRCLFQ
Sbjct: 400 PCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKILLSRCLFQ 459

Query: 187 DVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVV 246
           DVVKD RGRII CKMHDLIHD+AC VSNDQK  LD  +  D+EL  N ++   SKLR + 
Sbjct: 460 DVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLDSCTSSDEELWRNRTKNIGSKLRMIA 519

Query: 247 FIEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKL 306
             E M H+ E   +HDKI + +CLRVL+MNS+SIYKLP SIG+LKHLRY+DISGC I+KL
Sbjct: 520 CHEGMPHRIE-KAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDISGCLIQKL 579

Query: 307 PESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQL 366
           P+SIVLLYNL+T L+I  + I +LPK+LR  VNLRH EFS+  F + K+MPP++ QL  L
Sbjct: 580 PKSIVLLYNLKT-LRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPHLSQLIHL 639

Query: 367 QTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFE 426
           QTLS F+ GF+K C++AELGPLKNLKG L +  LE+V S+EEAK+A  +EKE L EL  E
Sbjct: 640 QTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEKLRELVLE 699

Query: 427 RSLLSGTED--KTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECG 486
               + TED    HN  +VLEGL+PHK L  L+I  F  + L +NIFV++LVAI L +C 
Sbjct: 700 WGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVAISLVDCR 759

Query: 487 IC-ELSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNL 546
            C  L MLG L +LEEL  S L  ++++  + +G     R     +   + + +   +  
Sbjct: 760 NCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGNHSNQRALFPKVKIFKLIEVERLEQW 819

Query: 547 KLLPSKGAMLHLTRLSRLHVFQCPQL 565
           + +     +    +L  L + +CP+L
Sbjct: 820 EEVTVGAHVTTFPQLQVLEISKCPRL 842

BLAST of Lcy13g013850 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.9e-150
Identity = 312/566 (55.12%), Postives = 406/566 (71.73%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIFG 66
           +VTTRS EVAK++ET P HHLG+LS DQCWS+F++SANANG++M S+++ +++ELVK  G
Sbjct: 307 LVTTRSAEVAKIMETLPSHHLGKLSDDQCWSLFQESANANGLSMTSSLKVVREELVKKIG 366

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ-ENFVSSILKLSVDHLPSSSLKQCF 126
           GLPL+A+V G A ++ GD  +WV  L+S+V   LQ EN  +S LKLSVD LP  SLKQCF
Sbjct: 367 GLPLIAKVWGSAEQYLGDPDKWVINLKSLVCNSLQNENSATSALKLSVDRLPRHSLKQCF 426

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDVV 186
           AY SNFPKDFEF KEQLIQ W+AQGFI   + R+ N+T+E+ G+ +F ILL RCLFQDVV
Sbjct: 427 AYCSNFPKDFEFTKEQLIQMWIAQGFIHLPKGRS-NETIEDEGDRHFKILLRRCLFQDVV 486

Query: 187 KDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFIE 246
           +DDRG I+ CKMHDLIHDIAC VSND KLQ   +SL D E+  + ++K  SKLRT+++ E
Sbjct: 487 QDDRGIIVSCKMHDLIHDIACDVSNDHKLQYSNNSL-DGEVWTDENKKIASKLRTIIYCE 546

Query: 247 -KMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLPE 306
            +M+      VIHDK++N +CLRVL MNS+SI +LP+SIG+LKHLRYLDISGC I +LP+
Sbjct: 547 DQMMPPIMEKVIHDKVMNFVCLRVLKMNSRSISELPDSIGELKHLRYLDISGCSITQLPK 606

Query: 307 SIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQT 366
           SI LLYNLQT L +   LI DLPKN+ D VNL+H +F  + F +T +MP   G+LTQLQT
Sbjct: 607 SIALLYNLQT-LNMGGLLIKDLPKNIIDLVNLKHLQFYPYHF-DTYKMPAYFGKLTQLQT 666

Query: 367 LSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFERS 426
           LS F+ GF+ G K++ELG LKNLKG LNL  LE+V SKEEA+ AKL+EKENL EL FE S
Sbjct: 667 LSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKEEARAAKLVEKENLDELEFEWS 726

Query: 427 LLSGTEDK-THNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECGICE 486
                +D  ++   E+LEGL+PHKNL+ L+I+    K LP+NIFVE+L  I L +C  CE
Sbjct: 727 QSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSLPNNIFVENLAVISLRDCQNCE 786

Query: 487 -LSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNLKLL 546
            L MLGQL NLE L    L+ +Q++S + +G  +  +  L     LR   LS  +NL+  
Sbjct: 787 RLPMLGQLRNLERLEVYGLHSVQSISNEFYGN-DCNQRTL--FPKLRAFLLSDMENLEQW 846

Query: 547 PSKGAMLHLTR---LSRLHVFQCPQL 565
                  +LT    L  L++++CP+L
Sbjct: 847 KEVTVASNLTTFPCLESLNIWRCPKL 865

BLAST of Lcy13g013850 vs. ExPASy TrEMBL
Match: A0A6J1DEU3 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111019843 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 2.6e-144
Identity = 305/555 (54.95%), Postives = 387/555 (69.73%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIFG 66
           +VTTRS +VAK+VET P HHL  LS DQCWS+FK+SA A G++M S++E +++ELVK FG
Sbjct: 43  VVTTRSADVAKIVETLPSHHLRELSDDQCWSLFKESAGAYGLSMNSSLEVIREELVKKFG 102

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ-ENFVSSILKLSVDHLPSSSLKQCF 126
           G+P+VARVLGRAVKFEGDY RW+ IL+S+V+TPLQ E FV SILKLSVD LPSSSLKQCF
Sbjct: 103 GIPVVARVLGRAVKFEGDYDRWMTILKSIVRTPLQEEKFVLSILKLSVDRLPSSSLKQCF 162

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDVV 186
           AY SNFPKD+ F KE+LI+ W+AQGFIQ ++ER  N +ME IG+MYF+ILLSRCLFQD V
Sbjct: 163 AYCSNFPKDYIFYKEELIRMWIAQGFIQLQQER-INLSMENIGDMYFNILLSRCLFQDSV 222

Query: 187 KDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFIE 246
           KDD+GRII+ KMHDL+HDI+  V ND+ LQLD  +L  +E     SE   SK RT+V  E
Sbjct: 223 KDDKGRIIHVKMHDLVHDISYEVLNDKILQLDLMNLLKEEHWKKESETIASKFRTMVCYE 282

Query: 247 KMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGC------PI 306
            MLH +E   I DKI+  + LRVL+M  + I+KLPNSIG+LKHLRYL I G        I
Sbjct: 283 DMLHNEE-KTIGDKIVKFVHLRVLIMEPEYIHKLPNSIGRLKHLRYLHIYGYSILGTHTI 342

Query: 307 EKLPESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQL 366
           +K+PESI LLYNLQT L+I++  +VDL KNLR+ V+LRH EFS   F   K MPP + +L
Sbjct: 343 DKVPESIALLYNLQT-LRIENLPMVDLSKNLRELVSLRHLEFSMNNFVANK-MPPYLSKL 402

Query: 367 TQLQTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCEL 426
           T+LQTLSNF+ G++KGCK+ ELG LKNLKG L L  LEQV SKEEA +AKL++KENL  L
Sbjct: 403 TKLQTLSNFVVGYEKGCKITELGFLKNLKGSLKLYCLEQVKSKEEASSAKLVDKENLDSL 462

Query: 427 TFERSLLSGTED-KTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESL-VAIHLH 486
            F      G       N LEVLEGL+PHKNLQ L I  F  K LP+ IFVE+L   +++ 
Sbjct: 463 AFHWINGYGIRKVNNQNDLEVLEGLQPHKNLQSLEIWKFEGKFLPNKIFVENLETRLYIR 522

Query: 487 ECGICELSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLT---------SL 543
           E  I    +LG+    +    +F   L+  ++     +++L  W   +T          L
Sbjct: 523 EL-IGVRRILGEFYGNDSKQRAFFPKLEEFTLI---CMDSLERWEEEVTVASNATTFPHL 582

BLAST of Lcy13g013850 vs. NCBI nr
Match: XP_022158717.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 588.2 bits (1515), Expect = 8.0e-164
Identity = 336/582 (57.73%), Postives = 419/582 (71.99%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNMELQQELVKIFGG 66
           +VTTRS EVAK++ET P HHL +LS DQCWS+F +SAN NG+++ S++++ QELVK  GG
Sbjct: 305 LVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVVQELVKKIGG 364

Query: 67  LPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ---ENFVSSILKLSVDHLPSSSLKQC 126
           +PLVARVLGRAVKFEGDY  WV  LES+++T LQ   E+FV SILKLSVD LP SSLKQC
Sbjct: 365 IPLVARVLGRAVKFEGDYETWVTTLESIIRTRLQLQEESFVLSILKLSVDRLPLSSLKQC 424

Query: 127 FAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDV 186
           F+Y SNFPKDF F+KE LI+ WMAQGFIQ +E R+ NKTME+ G+ YF ILLSRCLFQD+
Sbjct: 425 FSYCSNFPKDFVFEKEPLIRMWMAQGFIQLQERRS-NKTMEDEGDTYFKILLSRCLFQDI 484

Query: 187 VKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCING-SEKFRSKLRTVVF 246
           +KD +GRII  KMHDLIHDIAC +SND+KL LDP +L D E   N  SEK  SKLRTV+ 
Sbjct: 485 IKDGKGRIISFKMHDLIHDIACNISNDKKLHLDPCNLLDNEHWRNEISEKVASKLRTVIC 544

Query: 247 IEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLP 306
             + LH++E  VI DK+ N I LRVL++N  +  KLP+SIGKLKHLRYLDISGC +++LP
Sbjct: 545 RRETLHRRE-QVIDDKMSNFIWLRVLIINFWNAKKLPDSIGKLKHLRYLDISGCSMDELP 604

Query: 307 ESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDF-GFEETKQMPPNMGQLTQL 366
           ESIV LY+LQT L+I+ T+IVDLPKNL   V+LRH EFS F G ++  QMP ++ QL QL
Sbjct: 605 ESIVSLYHLQT-LRIEGTMIVDLPKNLGKLVSLRHLEFSFFLGIKKILQMPSHLSQLVQL 664

Query: 367 QTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFE 426
           QTLS FI GF+KG K+ ELGPLKNLKG LNL  LE++ SKEEA  AKL EKENL ELTF 
Sbjct: 665 QTLSTFIVGFEKGRKITELGPLKNLKGSLNLSNLERIKSKEEAMFAKLGEKENLHELTFT 724

Query: 427 RSLLSGTEDKTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECGIC 486
            +  S  E   HN L+VLEGL+PHK+LQ L+I+ F  + LP+ IFVE+LVAI L +C  C
Sbjct: 725 WTCSSEREYNNHNDLDVLEGLQPHKSLQSLSIQSFLGECLPNKIFVENLVAISLVDCENC 784

Query: 487 E-LSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNLKL 546
           E L MLGQLTNLEEL  S L+ ++++  + +G     R +      L+  +L+   NL+ 
Sbjct: 785 ERLPMLGQLTNLEELCISGLHSVRSIGNEFYGNDSDQRTF---FPKLKKFTLNKMSNLE- 844

Query: 547 LPSKGAMLHLTRLSRLHVFQCPQLRLGQSHTERAKLFHLPTI 583
                    +T    L VF C ++      +E  KL  +P I
Sbjct: 845 -----QWEEVTLAPNLTVFPCIEM---WKISECFKLLKVPKI 871

BLAST of Lcy13g013850 vs. NCBI nr
Match: XP_022944235.1 (disease resistance protein RGA2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 553.5 bits (1425), Expect = 2.2e-153
Identity = 311/558 (55.73%), Postives = 395/558 (70.79%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVE-TFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIF 66
           +VTTR+ EV K++E   P HH+ +LS +QCWS+F +SANANG+   SN+E +++ELVK F
Sbjct: 308 LVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKF 367

Query: 67  GGLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ---ENFVSSILKLSVDHLPSSSLK 126
           GG+PLVARVLG AVKFE DY  WV +LE V+++PL    +NF   ILKLSVD+LP SSLK
Sbjct: 368 GGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDYLP-SSLK 427

Query: 127 QCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQ 186
            CFAY SNFPKD+EF+K+ LIQ W+AQGFIQ +E R+C +T+EE GE YF ILLSRCLFQ
Sbjct: 428 PCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKILLSRCLFQ 487

Query: 187 DVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVV 246
           DVVKD RGRII CKMHDLIHD+AC VSNDQK  LD  +  D+EL  N ++   SKLR + 
Sbjct: 488 DVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLDSCTSSDEELWRNRTKNIGSKLRMIA 547

Query: 247 FIEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKL 306
             E M H+ E   +HDKI + +CLRVL+MNS+SIYKLP SIG+LKHLRY+DISGC I+KL
Sbjct: 548 CHEGMPHRIE-KAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDISGCLIQKL 607

Query: 307 PESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQL 366
           P+SIVLLYNL+T L+I  + I +LPK+LR  VNLRH EFS+  F + K+MPP++ QL  L
Sbjct: 608 PKSIVLLYNLKT-LRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPHLSQLIHL 667

Query: 367 QTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFE 426
           QTLS F+ GF+K C++AELGPLKNLKG L +  LE+V S+EEAK+A  +EKE L EL  E
Sbjct: 668 QTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEKLRELVLE 727

Query: 427 RSLLSGTED--KTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECG 486
               + TED    HN  +VLEGL+PHK L  L+I  F  + L +NIFV++LVAI L +C 
Sbjct: 728 WGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVAISLVDCR 787

Query: 487 IC-ELSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNL 546
            C  L MLG L +LEEL  S L  ++++  + +G          +   LR L +  C  L
Sbjct: 788 NCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGN--------HSNQHLRVLQILECSKL 847

Query: 547 KLLPSKGAMLHLTRLSRL 557
             LP+     H  +  RL
Sbjct: 848 TKLPNGLQFCHSIQHLRL 854

BLAST of Lcy13g013850 vs. NCBI nr
Match: XP_022944234.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 552.7 bits (1423), Expect = 3.7e-153
Identity = 307/566 (54.24%), Postives = 399/566 (70.49%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVE-TFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIF 66
           +VTTR+ EV K++E   P HH+ +LS +QCWS+F +SANANG+   SN+E +++ELVK F
Sbjct: 280 LVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKF 339

Query: 67  GGLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ---ENFVSSILKLSVDHLPSSSLK 126
           GG+PLVARVLG AVKFE DY  WV +LE V+++PL    +NF   ILKLSVD+LP SSLK
Sbjct: 340 GGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDYLP-SSLK 399

Query: 127 QCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQ 186
            CFAY SNFPKD+EF+K+ LIQ W+AQGFIQ +E R+C +T+EE GE YF ILLSRCLFQ
Sbjct: 400 PCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKILLSRCLFQ 459

Query: 187 DVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVV 246
           DVVKD RGRII CKMHDLIHD+AC VSNDQK  LD  +  D+EL  N ++   SKLR + 
Sbjct: 460 DVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLDSCTSSDEELWRNRTKNIGSKLRMIA 519

Query: 247 FIEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKL 306
             E M H+ E   +HDKI + +CLRVL+MNS+SIYKLP SIG+LKHLRY+DISGC I+KL
Sbjct: 520 CHEGMPHRIE-KAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDISGCLIQKL 579

Query: 307 PESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQL 366
           P+SIVLLYNL+T L+I  + I +LPK+LR  VNLRH EFS+  F + K+MPP++ QL  L
Sbjct: 580 PKSIVLLYNLKT-LRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPHLSQLIHL 639

Query: 367 QTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFE 426
           QTLS F+ GF+K C++AELGPLKNLKG L +  LE+V S+EEAK+A  +EKE L EL  E
Sbjct: 640 QTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEKLRELVLE 699

Query: 427 RSLLSGTED--KTHNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECG 486
               + TED    HN  +VLEGL+PHK L  L+I  F  + L +NIFV++LVAI L +C 
Sbjct: 700 WGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVAISLVDCR 759

Query: 487 IC-ELSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNL 546
            C  L MLG L +LEEL  S L  ++++  + +G     R     +   + + +   +  
Sbjct: 760 NCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGNHSNQRALFPKVKIFKLIEVERLEQW 819

Query: 547 KLLPSKGAMLHLTRLSRLHVFQCPQL 565
           + +     +    +L  L + +CP+L
Sbjct: 820 EEVTVGAHVTTFPQLQVLEISKCPRL 842

BLAST of Lcy13g013850 vs. NCBI nr
Match: XP_022148515.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 542.7 bits (1397), Expect = 3.8e-150
Identity = 312/566 (55.12%), Postives = 406/566 (71.73%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNME-LQQELVKIFG 66
           +VTTRS EVAK++ET P HHLG+LS DQCWS+F++SANANG++M S+++ +++ELVK  G
Sbjct: 307 LVTTRSAEVAKIMETLPSHHLGKLSDDQCWSLFQESANANGLSMTSSLKVVREELVKKIG 366

Query: 67  GLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ-ENFVSSILKLSVDHLPSSSLKQCF 126
           GLPL+A+V G A ++ GD  +WV  L+S+V   LQ EN  +S LKLSVD LP  SLKQCF
Sbjct: 367 GLPLIAKVWGSAEQYLGDPDKWVINLKSLVCNSLQNENSATSALKLSVDRLPRHSLKQCF 426

Query: 127 AYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDVV 186
           AY SNFPKDFEF KEQLIQ W+AQGFI   + R+ N+T+E+ G+ +F ILL RCLFQDVV
Sbjct: 427 AYCSNFPKDFEFTKEQLIQMWIAQGFIHLPKGRS-NETIEDEGDRHFKILLRRCLFQDVV 486

Query: 187 KDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVFIE 246
           +DDRG I+ CKMHDLIHDIAC VSND KLQ   +SL D E+  + ++K  SKLRT+++ E
Sbjct: 487 QDDRGIIVSCKMHDLIHDIACDVSNDHKLQYSNNSL-DGEVWTDENKKIASKLRTIIYCE 546

Query: 247 -KMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLPE 306
            +M+      VIHDK++N +CLRVL MNS+SI +LP+SIG+LKHLRYLDISGC I +LP+
Sbjct: 547 DQMMPPIMEKVIHDKVMNFVCLRVLKMNSRSISELPDSIGELKHLRYLDISGCSITQLPK 606

Query: 307 SIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQT 366
           SI LLYNLQT L +   LI DLPKN+ D VNL+H +F  + F +T +MP   G+LTQLQT
Sbjct: 607 SIALLYNLQT-LNMGGLLIKDLPKNIIDLVNLKHLQFYPYHF-DTYKMPAYFGKLTQLQT 666

Query: 367 LSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFERS 426
           LS F+ GF+ G K++ELG LKNLKG LNL  LE+V SKEEA+ AKL+EKENL EL FE S
Sbjct: 667 LSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKEEARAAKLVEKENLDELEFEWS 726

Query: 427 LLSGTEDK-THNYLEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECGICE 486
                +D  ++   E+LEGL+PHKNL+ L+I+    K LP+NIFVE+L  I L +C  CE
Sbjct: 727 QSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSLPNNIFVENLAVISLRDCQNCE 786

Query: 487 -LSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNLKLL 546
            L MLGQL NLE L    L+ +Q++S + +G  +  +  L     LR   LS  +NL+  
Sbjct: 787 RLPMLGQLRNLERLEVYGLHSVQSISNEFYGN-DCNQRTL--FPKLRAFLLSDMENLEQW 846

Query: 547 PSKGAMLHLTR---LSRLHVFQCPQL 565
                  +LT    L  L++++CP+L
Sbjct: 847 KEVTVASNLTTFPCLESLNIWRCPKL 865

BLAST of Lcy13g013850 vs. NCBI nr
Match: XP_038881404.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 536.2 bits (1380), Expect = 3.6e-148
Identity = 315/540 (58.33%), Postives = 386/540 (71.48%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANA--NGVTMFSNME-LQQELVKI 66
           IVTTRS EVA+++ET P  HL RLS DQCWS+FKK++NA  NG+++ S +E +++ELVK 
Sbjct: 311 IVTTRSNEVARIMETLPRLHLERLSDDQCWSLFKKTSNANTNGLSITSKLEVIREELVKK 370

Query: 67  FGGLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQ-ENFVSSILKLSVDHLPSSSLKQ 126
           FGG+PL+ARVLG A+KFEG Y  W+K LESV+  PLQ E+ V SILKLSVD LPSSSLKQ
Sbjct: 371 FGGIPLIARVLGAALKFEGVYEGWLKTLESVIDIPLQDEDLVLSILKLSVDRLPSSSLKQ 430

Query: 127 CFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQD 186
           CFAY SNFPKD++F+K++LIQ W+AQGF+Q +E R+ N  ME+IGE +F ILLSR LFQD
Sbjct: 431 CFAYCSNFPKDYKFEKKELIQMWIAQGFVQLQEGRS-NIKMEDIGEKFFDILLSRSLFQD 490

Query: 187 VVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGSEKFRSKLRTVVF 246
           VVKD +G I + KMHDLIHDIA  + N           + KE  I+ S K   KLRT++ 
Sbjct: 491 VVKDGKGGITHFKMHDLIHDIAGTIQN-----------FLKEFYIDESRKSSRKLRTLIC 550

Query: 247 IEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYKLPNSIGKLKHLRYLDISGCPIEKLP 306
             KM+HK     IHD I N   LRVL++NS S+  LPNSI KLKHLRYLDIS   IE+LP
Sbjct: 551 DRKMVHK----TIHDMISNNAHLRVLVVNS-SMKNLPNSIDKLKHLRYLDISNSEIEELP 610

Query: 307 ESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDFGFEETKQMPPNMGQLTQLQ 366
            SI+LL NLQ +LK+  +LI +LPK+L   VNLRH  FS    E+TKQMPP++ QLTQLQ
Sbjct: 611 HSIILLCNLQ-ILKL-GSLIKELPKSLGLLVNLRHLTFSIPFTEKTKQMPPHLSQLTQLQ 670

Query: 367 TLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEEAKTAKLLEKENLCELTFER 426
           TLS F+ GF+KGCK+ EL PLKNLKG L L  LEQV SKEEA++AKL EKENLCEL FE 
Sbjct: 671 TLSGFVVGFEKGCKIEELKPLKNLKGRLQLSNLEQVKSKEEARSAKLKEKENLCELFFEW 730

Query: 427 SLLSGTEDKTHNY--LEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVESLVAIHLHECGI 486
           SL    E   +NY   EVLEGL+PHKNLQFL+I++F  K LPH IFV+ L  IHL  C  
Sbjct: 731 SLHPERESSNNNYNDFEVLEGLQPHKNLQFLSIENFAGKLLPHCIFVDKLTVIHLRYCRR 790

Query: 487 CE-LSMLGQLTNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNLK 540
           CE L MLGQL+NLEEL  S L+H++++  + +GR      W      L+   LS   NLK
Sbjct: 791 CEVLPMLGQLSNLEELKISSLHHVKSIGNEFYGR-NGANHWAL-FPKLKKFILSQMHNLK 829

BLAST of Lcy13g013850 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 244.6 bits (623), Expect = 2.0e-64
Identity = 193/596 (32.38%), Postives = 315/596 (52.85%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNM--ELQQELVKIF 66
           +VTTRS  VA ++     H+L  LS   CWS+F K+   N     +    +L + +V   
Sbjct: 311 LVTTRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKC 370

Query: 67  GGLPLVARVLGRAVKFEGDYVRWVKILES-VVKTPLQENFVSSILKLSVDHLPSSSLKQC 126
            GLPL  + LG  ++FEG  + W ++L S +   P  ++ +  +L++S  +LP + LK+C
Sbjct: 371 RGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLP-AHLKRC 430

Query: 127 FAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILLSRCLFQDV 186
           FAY S FPK   F+K++++  WMA+GF+Q   +   +K +EE+G  YF  L SR L Q  
Sbjct: 431 FAYCSIFPKGHAFEKDKVVLLWMAEGFLQ---QTRSSKNLEELGNEYFSELESRSLLQKT 490

Query: 187 VKDDRGRIIYCKMHDLIHDIACGVSND--------QKLQLDPSSLWDKELCINGSEKFR- 246
               + R I   MHD I+++A   S +         KLQ+   + +   L  N +E    
Sbjct: 491 ----KTRYI---MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEF 550

Query: 247 SKLRTVVFIEKMLHKKEIN---------VIHDKIL-NLICLRVLLMNSQSIYKLPNSIGK 306
             LR V F+   L     N         ++ +K+L  L  LRVL ++   I +LP    K
Sbjct: 551 EALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFK 610

Query: 307 -LKHLRYLDISGCPIEKLPESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDF 366
            + H R+LD+S   +EKLP+S+  +YNLQTLL    + + +LP ++ + +NLR+    D 
Sbjct: 611 NISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYL---DL 670

Query: 367 GFEETKQMPPNMGQLTQLQTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVMSKEE 426
              + +QMP   G+L  LQTL+ F      G +++ELG L +L G L +++L++V+   +
Sbjct: 671 IGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVAD 730

Query: 427 AKTAKLLEKENLCELTFE-RSLLSGTED-----KTHNYLEVLEGLEPHKNLQFLNIKHFP 486
           A  A L  K++L E+ F  R+  S +E+     +T N  EV E L PH++++ L I+ + 
Sbjct: 731 AAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYK 790

Query: 487 NKQLPHNIFVES---LVAIHLHECGIC-ELSMLGQLTNLEELHNSFLYHLQNLSIQDFGR 546
            ++ P  +   S   +V I L EC  C  L  LGQL  L+ELH S +  LQ++  + +  
Sbjct: 791 GRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFS 850

Query: 547 IEALREW----LRNLTSLRTLSL-SFCKNLKLLPSKGAMLHLTRLSRLHVFQCPQL 565
            + LR+      R+L +LR  +L  + + L +  ++G +     L +L + +CP+L
Sbjct: 851 DQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDL--FPSLKKLFILRCPEL 890

BLAST of Lcy13g013850 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 179.5 bits (454), Expect = 8.0e-45
Identity = 164/542 (30.26%), Postives = 261/542 (48.15%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPGHHLGRLSHDQCWSIFKKSANANGVTMFSNMELQ---QELVKI 66
           ++TTRS  V+ + +    + +  +++++CW +  + A  N      N EL+   + + + 
Sbjct: 306 VLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQ 365

Query: 67  FGGLPLVARVLGRAVKFEGDYVRWVKILESVVKTPLQENFVSSILKLSVDHLPSSSLKQC 126
             GLPL AR +   ++ + +   W  + ++        N +  +LKLS D LP   LK+C
Sbjct: 366 CKGLPLAARAIASHLRSKPNPDDWYAVSKNFSS---YTNSILPVLKLSYDSLP-PQLKRC 425

Query: 127 FAYRSNFPKDFEFKKEQLIQTWMAQGFI-QPEEERTCNKTMEEIGEMYFHILLSRCLFQ- 186
           FA  S FPK   F +E+L+  WMA   + QP   R     +E+IG  Y   L+++  FQ 
Sbjct: 426 FALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSR----RLEDIGNDYLGDLVAQSFFQR 485

Query: 187 -DVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSL-----------WDKELCING 246
            D+       +    MHDL++D+A  VS D   +L+  ++           + +  C + 
Sbjct: 486 LDIT------MTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQC-DA 545

Query: 247 SEKFRS-----KLRTVVFIEKMLHKKEINVIHDKILN-----LICLRVLLMNSQSIYKLP 306
           S  FRS      LRT++        + +  + +K+LN     L  LR+L ++   I  LP
Sbjct: 546 SVAFRSICGAEFLRTILPFNSPTSLESLQ-LTEKVLNPLLNALSGLRILSLSHYQITNLP 605

Query: 307 NSIGKLKHLRYLDISGCPIEKLPESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFE 366
            S+  LK LRYLD+S   I++LPE +  L NLQTLL      +  LPK++ + +NLR   
Sbjct: 606 KSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLR--- 665

Query: 367 FSDFGFEETKQMPPNMGQLTQLQTLSNFIAGFDKGCKLAELGPLKNLKGGLNLLKLEQVM 426
             D       +MPP + +L  LQ LSNF+ G   G  L EL  L +L+G L + +L+ V 
Sbjct: 666 LLDLVGTPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVA 725

Query: 427 SKEEAKTAKLLEKENLCELTFERSLL-SGTEDKTHNYL-----EVLEGLEPHKNLQFLNI 486
              EAK A L  K  L  L  + ++  SG    + N L     EVL  LEPH +L+   I
Sbjct: 726 FASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCI 785

Query: 487 KHFPNKQLPHNIFVES---LVAIHLHECGIC-ELSMLGQLTNLEELHNSFLYHLQNLSIQ 512
           + +     P  +   S   + ++ L  C +C  L  +GQL +L+ L       LQ + + 
Sbjct: 786 ESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLD 828

BLAST of Lcy13g013850 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-32
Identity = 160/562 (28.47%), Postives = 256/562 (45.55%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPG--HHLGRLSHDQCWSIFKKSANANGVTMFSNMELQ---QELV 66
           IVTTRS  VAK V+      H    LS D  W +F   A A         EL+   +E+V
Sbjct: 293 IVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIV 352

Query: 67  KIFGGLPLVARVLGRAVKFEGD-YVRWVKILESVV-----KTPLQENFVSSILKLSVDHL 126
               GLPL  + +G  +  +   Y  W +I E         T   +N +SS L+LS D L
Sbjct: 353 TKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSS-LQLSYDEL 412

Query: 127 PSSSLKQCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILL 186
           P S LK C    S +P+D    K+QL+  W+ +GF+     R+      E GE  F  L 
Sbjct: 413 P-SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSAT----ESGEDCFSGLT 472

Query: 187 SRCLFQDVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGS----- 246
           +RCL + V K   G II CK+HD++ D+   ++       +P  L  + L I+G+     
Sbjct: 473 NRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFS-NPEGLNCRHLGISGNFDEKQ 532

Query: 247 EKFRSKLRTVVFIEKMLHKKEINV-IHDKILNLICLRVLLMNSQSIYKLP-----NSIGK 306
            K   KLR VV   K     ++N  +  K  +   LRVL + S+SI+  P     + I  
Sbjct: 533 IKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDI-SKSIFDAPLSEILDEIAS 592

Query: 307 LKHLRYLDISGC-PIEKLPESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDF 366
           L+HL  L +S   P+ + P S+  L+NLQ L       +  L   +  F  L   + ++ 
Sbjct: 593 LQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNC 652

Query: 367 GFEETKQMPPNMGQLTQLQTLSNF-IAGFDKGCKLAELGPLKNL-KGGLNLLKLEQVMSK 426
           G  E    P  +G L +L+ L  F  A  + GCKL+E+  L NL K GL+L + +Q+   
Sbjct: 653 GSLEC--FPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQI--- 712

Query: 427 EEAKTAKLLEKENLCELTFERSLLSGTEDKTHNYLEVLEGLEPHKNLQFLNIKHFPNKQL 486
           EE +   L+    L  ++     ++  +    + +  ++ L P   L  L+++ +P K  
Sbjct: 713 EEEELDSLINLSKLMSIS-----INCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSS 772

Query: 487 PHNIFVESLVAIHLHECGICELSMLGQLTNLEEL---HNSFLYHLQNLSIQDFGRIEALR 540
           P  +    L    L    IC     G L  ++E    + +  + ++ L +     ++   
Sbjct: 773 PSWLSPHKLPM--LRYMSICS----GNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDW 830

BLAST of Lcy13g013850 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-32
Identity = 160/562 (28.47%), Postives = 256/562 (45.55%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFPG--HHLGRLSHDQCWSIFKKSANANGVTMFSNMELQ---QELV 66
           IVTTRS  VAK V+      H    LS D  W +F   A A         EL+   +E+V
Sbjct: 293 IVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIV 352

Query: 67  KIFGGLPLVARVLGRAVKFEGD-YVRWVKILESVV-----KTPLQENFVSSILKLSVDHL 126
               GLPL  + +G  +  +   Y  W +I E         T   +N +SS L+LS D L
Sbjct: 353 TKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSS-LQLSYDEL 412

Query: 127 PSSSLKQCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHILL 186
           P S LK C    S +P+D    K+QL+  W+ +GF+     R+      E GE  F  L 
Sbjct: 413 P-SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSAT----ESGEDCFSGLT 472

Query: 187 SRCLFQDVVKDDRGRIIYCKMHDLIHDIACGVSNDQKLQLDPSSLWDKELCINGS----- 246
           +RCL + V K   G II CK+HD++ D+   ++       +P  L  + L I+G+     
Sbjct: 473 NRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFS-NPEGLNCRHLGISGNFDEKQ 532

Query: 247 EKFRSKLRTVVFIEKMLHKKEINV-IHDKILNLICLRVLLMNSQSIYKLP-----NSIGK 306
            K   KLR VV   K     ++N  +  K  +   LRVL + S+SI+  P     + I  
Sbjct: 533 IKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDI-SKSIFDAPLSEILDEIAS 592

Query: 307 LKHLRYLDISGC-PIEKLPESIVLLYNLQTLLKIKDTLIVDLPKNLRDFVNLRHFEFSDF 366
           L+HL  L +S   P+ + P S+  L+NLQ L       +  L   +  F  L   + ++ 
Sbjct: 593 LQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNC 652

Query: 367 GFEETKQMPPNMGQLTQLQTLSNF-IAGFDKGCKLAELGPLKNL-KGGLNLLKLEQVMSK 426
           G  E    P  +G L +L+ L  F  A  + GCKL+E+  L NL K GL+L + +Q+   
Sbjct: 653 GSLEC--FPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQI--- 712

Query: 427 EEAKTAKLLEKENLCELTFERSLLSGTEDKTHNYLEVLEGLEPHKNLQFLNIKHFPNKQL 486
           EE +   L+    L  ++     ++  +    + +  ++ L P   L  L+++ +P K  
Sbjct: 713 EEEELDSLINLSKLMSIS-----INCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSS 772

Query: 487 PHNIFVESLVAIHLHECGICELSMLGQLTNLEEL---HNSFLYHLQNLSIQDFGRIEALR 540
           P  +    L    L    IC     G L  ++E    + +  + ++ L +     ++   
Sbjct: 773 PSWLSPHKLPM--LRYMSICS----GNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDW 830

BLAST of Lcy13g013850 vs. TAIR 10
Match: AT3G07040.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 132.9 bits (333), Expect = 8.6e-31
Identity = 163/636 (25.63%), Postives = 284/636 (44.65%), Query Frame = 0

Query: 7   IVTTRSFEVAKMVETFP------GHHLGRLSHDQCWSIFKKSANANGVTMFSNMELQ--- 66
           ++TTR   VA    +FP       H +  L  D+ W +F   A    +       L+   
Sbjct: 311 MMTTRDMNVA----SFPYGIGSTKHEIELLKEDEAWVLFSNKAFPASLEQCRTQNLEPIA 370

Query: 67  QELVKIFGGLPLVARVLGRAV---KFEGDYVRWVKILESVVKTPLQENFVSSILKLSVDH 126
           ++LV+   GLPL    LG  +   KFE ++ +    L   +    +   V SI+ LS + 
Sbjct: 371 RKLVERCQGLPLAIASLGSMMSTKKFESEWKKVYSTLNWELNNNHELKIVRSIMFLSFND 430

Query: 127 LPSSSLKQCFAYRSNFPKDFEFKKEQLIQTWMAQGFIQPEEERTCNKTMEEIGEMYFHIL 186
           LP   LK+CF Y S FP ++  K+++LI+ WMAQ F++P          EE+ + Y + L
Sbjct: 431 LP-YPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIR----GVKAEEVADSYLNEL 490

Query: 187 LSRCLFQDVVKDDRGRIIYCKMHDLIHDIA---------CGVSNDQKLQLDPSSLWD--- 246
           + R + Q ++ +  GR    KMHD+I +IA         C V ND     D +   +   
Sbjct: 491 VYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDSDGDDAAETMENYG 550

Query: 247 -KELCINGS---EKFR-SKLRTVVFIEKMLHKKEINVIHDKILNLICLRVLLMNSQSIYK 306
            + LCI      +  R + L +++      HK E+      + +L  LR L +   SI K
Sbjct: 551 SRHLCIQKEMTPDSIRATNLHSLLVCSSAKHKMEL------LPSLNLLRALDLEDSSISK 610

Query: 307 LPNSIGKLKHLRYLDISGCPIEKLPESIVLLYNLQTLLKIKDTLIVDLP------KNLRD 366
           LP+ +  + +L+YL++S   +++LP++   L NL+T L  K + I +LP      K LR 
Sbjct: 611 LPDCLVTMFNLKYLNLSKTQVKELPKNFHKLVNLET-LNTKHSKIEELPLGMWKLKKLRY 670

Query: 367 FVNLRHFE--FSDFGFEETKQMPPNMGQLTQLQTLSNFIAGFD----KGC--KLAELGPL 426
            +  R  E   S++ +    ++ P + QL  LQ +  F A  +     GC  +L  +  +
Sbjct: 671 LITFRRNEGHDSNWNYVLGTRVVPKIWQLKDLQVMDCFNAEDELIKNLGCMTQLTRISLV 730

Query: 427 -------KNLKGGLNLLKLEQVMSKEEAKTAKLLEKENL-CELTFERSLLSGTEDKTHNY 486
                  ++L   LN +K  + +S       + LE ++L    + E+  L+G  ++  ++
Sbjct: 731 MVRREHGRDLCDSLNKIKRIRFLSLTSIDEEEPLEIDDLIATASIEKLFLAGKLERVPSW 790

Query: 487 LEVLEGLEPHKNLQFLNIKHFPNKQLPHNIFVE-----SLVAIHLHECGICELSMLGQ-L 546
              L+      NL +L ++     QL  N  +       LV +  +   +       Q  
Sbjct: 791 FNTLQ------NLTYLGLR---GSQLQENAILSIQTLPRLVWLSFYNAYMGPRLRFAQGF 850

Query: 547 TNLEELHNSFLYHLQNLSIQDFGRIEALREWLRNLTSLRTLSLSFCKNLKLLPSKGA--M 583
            NL+ L    + HL  + I+D    E           L+ L +  C+ L+ +P +G   +
Sbjct: 851 QNLKILEIVQMKHLTEVVIEDGAMFE-----------LQKLYVRACRGLEYVP-RGIENL 909