Lcy12g002930 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g002930 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 5.3e-120
Identity = 301/813 (37.02%), Postives = 423/813 (52.03%), Query Frame = 0

Query: 1   MEC---KRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTE 60
           MEC   K+     V P+FY VDPS VR Q GSF EAF+ + +      DK+ RWR ALTE
Sbjct: 105 MECHKDKKDPGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENL---KDKIPRWRTALTE 164

Query: 61  AANLAGFELKNTAHGHEGEFIREIVEKVSRDLN-RTLHVAHYQVGIESHVDAITSNLDVE 120
           AANL+G+ L++   G+E   I+EI + + R L  + L      VGI+SHV  +   L +E
Sbjct: 165 AANLSGWPLQD---GYESNQIKEITDSIFRRLKCKRLDAGANLVGIDSHVKEMIWRLHME 224

Query: 121 LGGVRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP---VRLQEKLFKA 180
              VRM+G++G+GG+GKTTIAK +YN+L + FE   FL NI++ F       LQ +L   
Sbjct: 225 SSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDD 284

Query: 181 LLKSSRIE-LQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIII 240
           +LK    + + +V  G  +++  L  K V +VLDD+DD  QL+ L R REW G GSR+II
Sbjct: 285 ILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVII 344

Query: 241 TTRDEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLP 300
           TTR++ +L    VD +Y V  ++  EA +LFS YAF QN P  DY  LS  VV YC GLP
Sbjct: 345 TTRNKHVLDVQKVDDLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLP 404

Query: 301 LALQVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFF 360
           LAL+VLG  L  + + EWE  L KL   P  +I   LK SYDGL  +T K+IFLDV+CFF
Sbjct: 405 LALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGL-GRTEKSIFLDVACFF 464

Query: 361 IGMDKNKVD----------------------VT---NKLMMHDLLRDMGREIVLNESPNE 420
            G D++ V                       +T   N++ MHDL++ MG EIV  + P+E
Sbjct: 465 KGEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDE 524

Query: 421 PGKRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYA 480
           P K SRL+   +    L   KG   +E +SL + K      ++ AF+ M +LR L++  +
Sbjct: 525 PNKWSRLWDTCDFERALTAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQ-S 584

Query: 481 GIQGDFK-----------------------------HFPQ-ELRWLCWHGFPLKFLPEEF 540
            +  DF+                              FP  ELR+L W G+PL FLP  F
Sbjct: 585 SLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNF 644

Query: 541 NLEKVVVIDLSYSHLTNFFRKKQKFSLENLTVLNLSHSHYLKCTPNFLKTPHLEELKLEY 600
           +  K+V + L  S++     +     LE L V++LS+S  L     F   P+LE L L  
Sbjct: 645 DGGKLVELHLKCSNIKQL--RLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRG 704

Query: 601 CKSLVKVDDSIGSLKKLVYIDLSCCICLNNLPETL------------------------- 660
           C SL+ +  S+G++KKL  + L  C  L NLP+++                         
Sbjct: 705 CVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGG 764

Query: 661 ---SLKFLYADDTCIQELPRTIVELKKLDCLSVSRC--------KGPPSKSHVEISWPWI 715
              SL  L   +T I++LP +I +L+ L  L +S C        KG   KS  E+     
Sbjct: 765 NMKSLTELDLQNTAIKDLPDSIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDL--- 824

BLAST of Lcy12g002930 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.4e-117
Identity = 295/812 (36.33%), Postives = 422/812 (51.97%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC++ L   V P+FY VDPS VR Q GSF EAF+ +++      DK+ RWR ALTEAAN
Sbjct: 102 MECQKDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENW---KDKIPRWRTALTEAAN 161

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLN-RTLHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G+ L +    +E   I+EI   + R L  + L V    VGI+SHV  +   L +E   
Sbjct: 162 LSGWHLLDDR--YESNQIKEITNSIFRQLKCKRLDVGANLVGIDSHVKEMILRLHLESSD 221

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP---VRLQEKLFKALLK 180
           VRM+GI+G+GG+GKTTIAK +YN+L   FE   FL NI++   P     LQ +L   +L+
Sbjct: 222 VRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNPQVLFHLQNQLLGDILE 281

Query: 181 SSRIE-LQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
               + + +V     +++  L  ++V +VLDD+DD+ QL+ L   REW G GSR+IITTR
Sbjct: 282 GEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTR 341

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           ++ +L   +VD +Y V  ++  EA +LFS YAF QN P  DY  L+  VV YC GLPLAL
Sbjct: 342 NKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLAL 401

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L  + + +WE  L KL + P  DI + LK SYDGL D+  KNIFLD++CFF G 
Sbjct: 402 KVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSYDGL-DRIDKNIFLDLACFFKGE 461

Query: 361 DKNKV-------------------DVT------NKLMMHDLLRDMGREIVLNESPNEPGK 420
            ++ V                   D+       N++ MHDL++ MG EIV    P EP K
Sbjct: 462 GRDFVLRILDGCDFPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNK 521

Query: 421 RSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQL----NY 480
            SRL+   +    L   +G   +E +SL + K      ++  F+ M KLR L++    + 
Sbjct: 522 WSRLWDPCDFERALTADEGIKSVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDI 581

Query: 481 AGIQGD------------------------FKHFPQELRWLCWHGFPLKFLPEEFNLEKV 540
               GD                        FK    ELR+L W G+PL  LP  F+  K+
Sbjct: 582 DSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKL 641

Query: 541 VVIDLSYSHLTNFFRKKQKFSLENLTVLNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLV 600
           V + L  S++   ++  +   LE L V++LS+S  L     F   P+LE L L  C SL+
Sbjct: 642 VELHLKCSNIKQLWQGHK--DLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLI 701

Query: 601 KVDDSIGSLKKLVYIDLSCCICLNNLPETL----SLKFLYAD------------------ 660
            +  S+G++KKL  + L  C  L NLP+++    SL+ LY                    
Sbjct: 702 DIHPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSL 761

Query: 661 ------DTCIQELPRTIVELKKLDCLSVSRC--------KGPPSKSHVEISWPWIFPRQV 719
                 +T I++LP +I +L+ L+ L +S C        KG   KS  E+       +  
Sbjct: 762 TELDLKNTAIKDLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDL-----KNT 821

BLAST of Lcy12g002930 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.8e-113
Identity = 263/772 (34.07%), Postives = 417/772 (54.02%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           +E       +V+P+FYDVDPS VR QNG +   F++ +   V D DKV+RWR ALT+ AN
Sbjct: 91  LEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVDDRDKVLRWREALTKVAN 150

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRTLHVAHYQ-VGIESHVDAITSNLDVELGG 120
           ++G +L+NT +G E + I++I++ +      ++ + +   VGIES +  ++S L ++L G
Sbjct: 151 ISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRDLVGIESQIKKLSSLLRMDLKG 210

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP---VRLQEKLFKALLK 180
           VR++GIWGMGGVGKTT A+A++N+ Y++FE ACFL ++K+  +    + LQ+ L   LLK
Sbjct: 211 VRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVKEYLQHHTLLYLQKTLLSKLLK 270

Query: 181 SSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTRD 240
              ++  + +E   +L++RL  KKVL+VLDD++   QL  L  + +WFG+GSRI+ITTRD
Sbjct: 271 VEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRD 330

Query: 241 EQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLALQ 300
            +LLKN DV   Y +  +++ EA++LF+ +AF ++ P K++ +L   VV Y GGLPLAL+
Sbjct: 331 MKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGGLPLALK 390

Query: 301 VLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGMD 360
           VLGS L+   +  W   +++LK  P  +I   LKIS+DGL+D   K+IFLD++CFF G +
Sbjct: 391 VLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKISFDGLRDY-EKSIFLDIACFFRGYN 450

Query: 361 KNKVD-------------------------VTNKLMMHDLLRDMGREIVLNESPNEPGKR 420
           +  +                          + +K+ MHDL+++MGR+I + ESP      
Sbjct: 451 QRDMTALFHASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQESP-----M 510

Query: 421 SRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNK-----VLHTKAFSAMQKLRYLQLNY 480
            R++  ++V          + IEGL L  P+   +     +   +A    ++LR L   Y
Sbjct: 511 RRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEY 570

Query: 481 --AGIQGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSL 540
              G      + P  L WL W  +     P  F   K+V + +  S +   +   ++ + 
Sbjct: 571 YNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPSKLVYLTMKGSSIIELWNGAKRLAF 630

Query: 541 ENLTVLNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCIC 600
             LT L+LS+ H L  TP+F    +LE L L  C +LV+V  S+G LK L+ +++  CI 
Sbjct: 631 --LTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCIS 690

Query: 601 LNNLP--------ETLSLKFLYADDTCIQELPRTIVELKKLDCLSVSRCKGPPSKSHVEI 660
           L  LP        E L L + + +     E+ R +  LKKLD  S    + P S  H+  
Sbjct: 691 LERLPAIIQSECLEVLDLNYCF-NLKMFPEVERNMTHLKKLDLTSTGIRELPASIEHLS- 750

Query: 661 SWPWIFPRQVSISIVLPTSL-----------RGLESLTELN-LSHCN-------LSDDAI 709
           S   +     +  + LP+S+             L SL E++  S+C        +S   +
Sbjct: 751 SLENLQMHSCNQLVSLPSSIWRFRNLKISECEKLGSLPEIHGNSNCTRELILKLVSIKEL 810

BLAST of Lcy12g002930 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 4.5e-111
Identity = 286/829 (34.50%), Postives = 416/829 (50.18%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MECK    Q V+P+FYDVDPS VRNQ  SF +AF  H+ +   D + + RWR+AL EAAN
Sbjct: 91  MECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYKDDVEGIQRWRIALNEAAN 150

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNR-TLHVAHYQVGIESHVDAITSNLDVELGG 120
           L G    +     + + IR+IV+++S  L + +L      VGI++H++ I S L++ + G
Sbjct: 151 LKG--SCDNRDKTDADCIRQIVDQISSKLCKISLSYLQNIVGIDTHLEKIESLLEIGING 210

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLY------KHFEGACFLGNIKDTFEPVR-LQEKLFK 180
           VR++GIWGMGGVGKTTIA+A+++ L         F+GACFL +IK+    +  LQ  L  
Sbjct: 211 VRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKENKRGMHSLQNALLS 270

Query: 181 ALLKSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQ-LQALGRSREWFGTGSRII 240
            LL+  +    N ++G   +  RLR KKVL+VLDDID+    L+ L    +WFG GSRII
Sbjct: 271 ELLR-EKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRII 330

Query: 241 ITTRDEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGL 300
           ITTRD+ L++  D+  IY V  + + E++QLF  +AF +  P++++ +LS  VV Y  GL
Sbjct: 331 ITTRDKHLIEKNDI--IYEVTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGL 390

Query: 301 PLALQVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCF 360
           PLAL+V GS LH   + EW+ A+  +K   +  I +KLKISYDGL+ +  + +FLD++CF
Sbjct: 391 PLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPK-QQEMFLDIACF 450

Query: 361 FIG------------------------MDKNKVDVT--NKLMMHDLLRDMGREIVLNESP 420
             G                        +DK+ V ++  N++ MHDL++DMG+ IV     
Sbjct: 451 LRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIV--NFQ 510

Query: 421 NEPGKRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLN 480
            +PG+RSRL+   EV  V+    GT  +E + +    SS      +A   M++LR   + 
Sbjct: 511 KDPGERSRLWLAKEVEEVMSNNTGTMAMEAIWVS-SYSSTLRFSNQAVKNMKRLRVFNMG 570

Query: 481 YAGIQGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLE 540
            +       + P  LR      +P +  P  F L+ +V + L ++ L + + + +   L 
Sbjct: 571 RSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRHLWTETK--HLP 630

Query: 541 NLTVLNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLV---------- 600
           +L  ++LS S  L  TP+F   P+LE + L  C +L +V  S+G   K++          
Sbjct: 631 SLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSL 690

Query: 601 ------------YIDLSCCICLNNLPE-----------------------------TLSL 660
                       Y+ L  C  L  LPE                             T   
Sbjct: 691 KRFPCVNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVT 750

Query: 661 KFLYADDTCIQELPRTIVELKKLDCLSVSRCK---------GPPSKSHVEISWPWIFPRQ 716
           K L  +   +  LP +I  LK L  LSVS C          G      V  +   +  R 
Sbjct: 751 KLLLWNMKNLVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRP 810

BLAST of Lcy12g002930 vs. ExPASy Swiss-Prot
Match: Q9FI14 (Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.1e-96
Identity = 256/775 (33.03%), Postives = 404/775 (52.13%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           M+C+  L Q V+ +FYDVDPSDVR Q G F + F +         + V RW+ ALT AAN
Sbjct: 118 MKCREELGQTVMTVFYDVDPSDVRKQKGDFGKVFKKTCVGR--PEEMVQRWKQALTSAAN 177

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRTLHVAHYQ-----VGIESHVDAITSNLDV 120
           + G + +N       E   +++ K+S+D++  L     +     VGIE+H   ITS L +
Sbjct: 178 ILGEDSRN------WENEADMIIKISKDVSDVLSFTPSKDFDEFVGIEAHTTEITSLLQL 237

Query: 121 ELGGVRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTF-----EPVRLQEKL 180
           +L  VRM+GIWG  G+GKTTI++ +YN+L+  F+    + NIK  +     +    + +L
Sbjct: 238 DLEEVRMIGIWGPAGIGKTTISRVLYNKLFHQFQLGAIIDNIKVRYPRPCHDEYSAKLQL 297

Query: 181 FKALLKSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRI 240
            K LL     +   V   + + Q+RL+ KKVLLVLDD+D + QL A+ +  +WFG GSRI
Sbjct: 298 QKELLSQMINQKDMVVPHLGVAQERLKDKKVLLVLDDVDGLVQLDAMAKDVQWFGLGSRI 357

Query: 241 IITTRDEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGG 300
           I+ T+D +LLK   +  IY V+     EAL++F  YAF +  P   + Q++++V    G 
Sbjct: 358 IVVTQDLKLLKAHGIKYIYKVDFPTSDEALEIFCMYAFGEKSPKVGFEQIARTVTTLAGK 417

Query: 301 LPLALQVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSC 360
           LPL L+V+GS L     +EW  ++ +L+T    DI+  LK SY+ L +Q  K++FL ++C
Sbjct: 418 LPLGLRVMGSYLRRMSKQEWAKSIPRLRTSLDDDIESVLKFSYNSLAEQ-EKDLFLHITC 477

Query: 361 FF---------IGMDKNKVDVTNKLM----------------MHDLLRDMGREIVLNESP 420
           FF         + + K  VD+   L                 MH+LL  +G +IV  +S 
Sbjct: 478 FFRRERIETLEVFLAKKSVDMRQGLQILADKSLLSLNLGNIEMHNLLVQLGLDIVRKQSI 537

Query: 421 NEPGKRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLH--TKAFSAMQKLRYLQ 480
           ++PGKR  L   +++  VL    GT  + G+ L++      V++   +AF  M  L++L+
Sbjct: 538 HKPGKRQFLVDTEDICEVLTDDTGTRTLIGIDLELSGVIEGVINISERAFERMCNLQFLR 597

Query: 481 LNYAGIQGD-----------FKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHL 540
            ++    GD             H  ++LR L W  +PL  LP +FN E +V I++  S L
Sbjct: 598 FHHP--YGDRCHDILYLPQGLSHISRKLRLLHWERYPLTCLPPKFNPEFLVKINMRDSML 657

Query: 541 TNFFRKKQKFSLENLTVLNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLK 600
              +   +   + NL  ++LS    LK  P+F    +L+EL+L  C SLV++  SIG+  
Sbjct: 658 EKLWDGNE--PIRNLKWMDLSFCVNLKELPDFSTATNLQELRLINCLSLVELPSSIGNAT 717

Query: 601 KLVYIDLSCCICLNNLPETL----SLKFLYADD-TCIQELPRTIVELKKLDCLSVSRCKG 660
            L+ +DL  C  L  LP ++    +LK L+ +  + + +LP +   +  L  L++S C  
Sbjct: 718 NLLELDLIDCSSLVKLPSSIGNLTNLKKLFLNRCSSLVKLPSSFGNVTSLKELNLSGCSS 777

Query: 661 ---PPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDIGSL 716
               PS     ++   ++    S  + LP+S+    +L EL+L +C+ S    P  + +L
Sbjct: 778 LLEIPSSIGNIVNLKKVYADGCSSLVQLPSSIGNNTNLKELHLLNCS-SLMECPSSMLNL 837

BLAST of Lcy12g002930 vs. ExPASy TrEMBL
Match: A0A6J1DR26 (TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022364 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.9e-266
Identity = 495/768 (64.45%), Postives = 563/768 (73.31%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDN-DKVVRWRMALTEAA 60
           MEC+RTLNQ+VLP+FYDVDPSDVRNQ GSF EAFSR+Q     +N +K++ WRMALTEAA
Sbjct: 92  MECRRTLNQLVLPVFYDVDPSDVRNQKGSFGEAFSRYQLHFGSENYNKILSWRMALTEAA 151

Query: 61  NLAGFELKNTAHGHEGEFIREIVEKVSRDLNRTLHVAHYQVGIESHVDAITSNLDVELGG 120
           NLAGF L+N A GHEG+FI++I++KVSRDL R + VAH+ VGI+ HV+AITS L V LGG
Sbjct: 152 NLAGFNLENIADGHEGKFIKKIIQKVSRDLKRIIDVAHHLVGIDFHVNAITSKLGVGLGG 211

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALL 180
           VRM+GIWGMGGVGKTTIAKAVYN LY  F+GACFL NIKDT E     V LQEKL K++ 
Sbjct: 212 VRMVGIWGMGGVGKTTIAKAVYNLLYDDFDGACFLDNIKDTSEQLNGLVHLQEKLLKSIF 271

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KSS+IELQ V EGI  LQQRL HKKVLL+LDDIDDM QL AL RSR WFG G+RIIITTR
Sbjct: 272 KSSKIELQYVHEGITQLQQRLPHKKVLLILDDIDDMGQLNALARSRCWFGAGTRIIITTR 331

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           +E  LKN  VDAI T++EM+E EAL+LFS +AFH   PS DY QLSK VV YCGGLPLAL
Sbjct: 332 NEHFLKNFQVDAICTIDEMNENEALELFSWHAFHNKYPSVDYCQLSKRVVSYCGGLPLAL 391

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L+GR V EWEDALNKLK IP  +I  KLK S+DGL DQ +K+IFLD+SCFFIGM
Sbjct: 392 EVLGSFLYGRTVLEWEDALNKLKKIPLDEISRKLKTSFDGLGDQAYKDIFLDISCFFIGM 451

Query: 361 DKN--------------------------KVDVTNKLMMHDLLRDMGREIVLNESPNEPG 420
           DK+                          +VD  NK  MHDLLRDMGREIV NES  EP 
Sbjct: 452 DKSEVTRILDGCGFFPIIGISVLLQRRLLRVDHENKFRMHDLLRDMGREIVHNESRKEPA 511

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           KRSR+F ND+VL VLEKQKG DV EGLSLK+  SS K  HTKAFS MQKLR LQLNYA I
Sbjct: 512 KRSRIFVNDDVLDVLEKQKGADVTEGLSLKLSISSKKNFHTKAFSEMQKLRLLQLNYARI 571

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
           +GDFKH  QELRW+CWHGFPL+ LP+ F+LEK V IDL YSHL  FF+K+ KFSLE LT+
Sbjct: 572 RGDFKHISQELRWICWHGFPLESLPKSFHLEKAVAIDLRYSHLIKFFQKEPKFSLEKLTI 631

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHS YL CTPNF K PHL++L+LE CKSLVKVDDSIG L+KL +I+L  CICLN LP
Sbjct: 632 LNLSHSRYLTCTPNFSKLPHLQKLELEDCKSLVKVDDSIGCLQKLGFINLKDCICLNKLP 691

Query: 601 ETL----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLS 660
           E                              SL  L AD TCIQELP TIV LKKL CLS
Sbjct: 692 EAFCELKSLERLILSGCSKLEKLPNELGKMESLTTLAADGTCIQELPSTIVGLKKLKCLS 751

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 710
           ++R     SKS  EI W  IFP + S SIVLP  L+G +SLT L L +CN+SDDAIPKDI
Sbjct: 752 ITRSNRLLSKSLAEILWSRIFPSKFSNSIVLPMCLQGFKSLTILRLRNCNVSDDAIPKDI 811

BLAST of Lcy12g002930 vs. ExPASy TrEMBL
Match: A0A6J1DLU8 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022364 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.9e-266
Identity = 495/768 (64.45%), Postives = 563/768 (73.31%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDN-DKVVRWRMALTEAA 60
           MEC+RTLNQ+VLP+FYDVDPSDVRNQ GSF EAFSR+Q     +N +K++ WRMALTEAA
Sbjct: 92  MECRRTLNQLVLPVFYDVDPSDVRNQKGSFGEAFSRYQLHFGSENYNKILSWRMALTEAA 151

Query: 61  NLAGFELKNTAHGHEGEFIREIVEKVSRDLNRTLHVAHYQVGIESHVDAITSNLDVELGG 120
           NLAGF L+N A GHEG+FI++I++KVSRDL R + VAH+ VGI+ HV+AITS L V LGG
Sbjct: 152 NLAGFNLENIADGHEGKFIKKIIQKVSRDLKRIIDVAHHLVGIDFHVNAITSKLGVGLGG 211

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALL 180
           VRM+GIWGMGGVGKTTIAKAVYN LY  F+GACFL NIKDT E     V LQEKL K++ 
Sbjct: 212 VRMVGIWGMGGVGKTTIAKAVYNLLYDDFDGACFLDNIKDTSEQLNGLVHLQEKLLKSIF 271

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KSS+IELQ V EGI  LQQRL HKKVLL+LDDIDDM QL AL RSR WFG G+RIIITTR
Sbjct: 272 KSSKIELQYVHEGITQLQQRLPHKKVLLILDDIDDMGQLNALARSRCWFGAGTRIIITTR 331

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           +E  LKN  VDAI T++EM+E EAL+LFS +AFH   PS DY QLSK VV YCGGLPLAL
Sbjct: 332 NEHFLKNFQVDAICTIDEMNENEALELFSWHAFHNKYPSVDYCQLSKRVVSYCGGLPLAL 391

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L+GR V EWEDALNKLK IP  +I  KLK S+DGL DQ +K+IFLD+SCFFIGM
Sbjct: 392 EVLGSFLYGRTVLEWEDALNKLKKIPLDEISRKLKTSFDGLGDQAYKDIFLDISCFFIGM 451

Query: 361 DKN--------------------------KVDVTNKLMMHDLLRDMGREIVLNESPNEPG 420
           DK+                          +VD  NK  MHDLLRDMGREIV NES  EP 
Sbjct: 452 DKSEVTRILDGCGFFPIIGISVLLQRRLLRVDHENKFRMHDLLRDMGREIVHNESRKEPA 511

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           KRSR+F ND+VL VLEKQKG DV EGLSLK+  SS K  HTKAFS MQKLR LQLNYA I
Sbjct: 512 KRSRIFVNDDVLDVLEKQKGADVTEGLSLKLSISSKKNFHTKAFSEMQKLRLLQLNYARI 571

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
           +GDFKH  QELRW+CWHGFPL+ LP+ F+LEK V IDL YSHL  FF+K+ KFSLE LT+
Sbjct: 572 RGDFKHISQELRWICWHGFPLESLPKSFHLEKAVAIDLRYSHLIKFFQKEPKFSLEKLTI 631

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHS YL CTPNF K PHL++L+LE CKSLVKVDDSIG L+KL +I+L  CICLN LP
Sbjct: 632 LNLSHSRYLTCTPNFSKLPHLQKLELEDCKSLVKVDDSIGCLQKLGFINLKDCICLNKLP 691

Query: 601 ETL----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLS 660
           E                              SL  L AD TCIQELP TIV LKKL CLS
Sbjct: 692 EAFCELKSLERLILSGCSKLEKLPNELGKMESLTTLAADGTCIQELPSTIVGLKKLKCLS 751

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 710
           ++R     SKS  EI W  IFP + S SIVLP  L+G +SLT L L +CN+SDDAIPKDI
Sbjct: 752 ITRSNRLLSKSLAEILWSRIFPSKFSNSIVLPMCLQGFKSLTILRLRNCNVSDDAIPKDI 811

BLAST of Lcy12g002930 vs. ExPASy TrEMBL
Match: A0A6J1DMG1 (TMV resistance protein N OS=Momordica charantia OX=3673 GN=LOC111022384 PE=4 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.9e-242
Identity = 449/776 (57.86%), Postives = 557/776 (71.78%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC+RTL Q+VLP+FYDVDPS VR Q   FE AF RH++R   D D+V+RWRMALTEAAN
Sbjct: 93  MECRRTLRQLVLPVFYDVDPSTVRKQTDGFEHAFLRHEERFFFDIDRVLRWRMALTEAAN 152

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRT-LHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G++L+N A GHE +FIR IV+K+SR+LN T L+VA Y VGIES    ITS L+    G
Sbjct: 153 LSGWDLRNVADGHEAKFIRLIVDKISRELNSTYLYVALYPVGIESRTKVITSQLEKGSDG 212

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFE----PVRLQEKLFKALL 180
           VRM+GIWGMGG GKTTIAKAVYNQLY +FEG CFL NIK+        VRLQE+L  ++ 
Sbjct: 213 VRMVGIWGMGGTGKTTIAKAVYNQLYHNFEGRCFLANIKEISNQPDGQVRLQEQLLCSIS 272

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KSS+ +L+NVD GI LLQ+R R KKVLL+LDD+D + QL A+ RSREWFG+GSRI+ITTR
Sbjct: 273 KSSKTKLENVDRGIVLLQERFRRKKVLLILDDVDQIGQLNAIARSREWFGSGSRIVITTR 332

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           D+ LL+ L VDAI +V+EM++TEAL+LFS +AF  + P +D+++LSK VV Y GGLPLAL
Sbjct: 333 DKHLLEQLRVDAICSVDEMNDTEALELFSWHAFRNSYPDEDFHELSKCVVDYSGGLPLAL 392

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L GR + EW+DALNKLKTIP   IQ KL+IS+DGL D T+K+IFLDVSCFFIGM
Sbjct: 393 EVLGSFLFGRSIPEWKDALNKLKTIPDDQIQRKLRISFDGLSDHTYKDIFLDVSCFFIGM 452

Query: 361 DKNKVDVT--------------------------NKLMMHDLLRDMGREIVLNESPNEPG 420
           D+N V+                            NKLMMHDLLRDMGREIV  + P EP 
Sbjct: 453 DRNYVEQVLDGCGFFPKIGISVLLQRCLLTIGDRNKLMMHDLLRDMGREIVREKHPKEPE 512

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           + SRL  +DEVL VL +QKGTD IEGL+LK+P+ S + L T+AF+ MQ LR LQLN+  +
Sbjct: 513 RHSRLLLHDEVLSVLTRQKGTDAIEGLALKLPRFSKEKLSTEAFNEMQNLRLLQLNFVNL 572

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
            GDFKH  QELRWLCWHGFPLKFLP++F++EK+V IDL YSH+  FF K+ KF LE L +
Sbjct: 573 NGDFKHLSQELRWLCWHGFPLKFLPKDFHIEKLVAIDLRYSHI-RFFWKESKF-LEKLKI 632

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHSH L  TP+FLK P LE+LKL+ CK+LV++  SIG LK LV+I+L  C  L +LP
Sbjct: 633 LNLSHSHDLTHTPDFLKLPSLEKLKLKDCKNLVELHHSIGELKGLVFINLKDCKRLKSLP 692

Query: 601 ETL----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLS 660
           E+                             SL  L ADDT IQ++P TIV+LK L  LS
Sbjct: 693 ESFSKLKSLETLTISGCSKINTLPEDLGELKSLITLIADDTAIQQVPSTIVKLKNLKYLS 752

Query: 661 VSRCKGPPSKSHVEISWPWIFP-RQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKD 717
           +  CKGPPSKS   + W WI P +Q   SIVLP SL+GL SL  L L++CNLS++ IPKD
Sbjct: 753 LCGCKGPPSKSLPSLFWSWISPKKQHPNSIVLPASLQGLNSLRTLRLNNCNLSNNTIPKD 812

BLAST of Lcy12g002930 vs. ExPASy TrEMBL
Match: A0A6J1F3C0 (TMV resistance protein N OS=Cucurbita moschata OX=3662 GN=LOC111441805 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 6.9e-240
Identity = 436/775 (56.26%), Postives = 552/775 (71.23%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC+RTL Q+VLP+FYDVDPS VR Q G FEEAF RH+QR   D D+V+RWRMAL+EAAN
Sbjct: 98  MECRRTLRQLVLPIFYDVDPSTVRKQTGGFEEAFLRHEQRFFTDIDRVLRWRMALSEAAN 157

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRT-LHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G++L+N A GHE +F+R IVEK+SR+LN T L +A Y VGIES V  + S+LD+   G
Sbjct: 158 LSGWDLRNIADGHEAKFVRLIVEKISRELNSTYLFIALYPVGIESRVKVVASHLDIGSNG 217

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFE----PVRLQEKLFKALL 180
           VR +GI GMGG+GKTTIAKA+YNQLY +FE  CFL NIKD  +     +RLQE+L  +L 
Sbjct: 218 VRFVGILGMGGMGKTTIAKALYNQLYHNFEAKCFLANIKDISKQPSGQIRLQEQLLSSLT 277

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KS++I+L+NVD GI +LQ+RLR KKVLL+LDD+D++ QL A+ RSREWFG+GSRI++TTR
Sbjct: 278 KSTKIKLENVDRGIVVLQERLRRKKVLLILDDVDEIGQLNAIARSREWFGSGSRIVLTTR 337

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           D+ LL  L+VD I +V+EMD+TEAL+LFS +AF  + PS+ +++LSK VV Y GGLPLAL
Sbjct: 338 DQHLLNQLEVDGICSVDEMDDTEALELFSWHAFRNSYPSETFHELSKRVVNYSGGLPLAL 397

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L GR   EWED LNKLKTIP   IQ KL+IS+DGL D T+K+IFLDVSCFFIG+
Sbjct: 398 EVLGSFLFGRSRAEWEDTLNKLKTIPDDQIQRKLRISFDGLNDHTYKDIFLDVSCFFIGV 457

Query: 361 DKNKVDVT--------------------------NKLMMHDLLRDMGREIVLNESPNEPG 420
           DKN V+                            NKLMMHDLLRDMGREIV  + P EP 
Sbjct: 458 DKNYVEQVLDGCGFFPKIGISVLLQRCLLTIGDKNKLMMHDLLRDMGREIVREKFPKEPE 517

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           + SRL  ++EV+ VL + KGT  IEGLSLK+P+ S + L +KAF+ MQ LR LQLN+  +
Sbjct: 518 RHSRLVLHEEVISVLSRHKGTYAIEGLSLKLPRFSKEKLSSKAFNEMQNLRLLQLNFVTL 577

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
            GDFKH  QE+RWLCWHGFPLKFLP++F++EK+V +DL +S +  FF K+ KF L+NL  
Sbjct: 578 TGDFKHISQEIRWLCWHGFPLKFLPKDFHMEKLVAVDLRHSQI-RFFWKESKF-LKNLKF 637

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHSHYL  TP+F K P+LE LKL+ CKSLV++  +IG LK+L+ I+L  C CL +LP
Sbjct: 638 LNLSHSHYLTYTPDFSKLPNLETLKLKDCKSLVELHPTIGELKRLILINLKDCKCLKSLP 697

Query: 601 ----------------------------ETLSLKFLYADDTCIQELPRTIVELKKLDCLS 660
                                       E +SL  L ADDT IQ +P TIV+LK L  LS
Sbjct: 698 KGFSMLKSLETLFLSGCSKLSTLPEDLGEMVSLITLTADDTAIQRIPSTIVKLKNLKYLS 757

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 717
           +  CKGPPSKS     W WI P +   SI+LP+SL+GL +L  L L++CNLS++ IPKDI
Sbjct: 758 LCGCKGPPSKSFPSAFWSWILPTKYPNSILLPSSLQGLNALRTLRLNNCNLSNNTIPKDI 817

BLAST of Lcy12g002930 vs. ExPASy TrEMBL
Match: A0A6J1J021 (TMV resistance protein N OS=Cucurbita maxima OX=3661 GN=LOC111482231 PE=4 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 2.7e-236
Identity = 435/775 (56.13%), Postives = 544/775 (70.19%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC+RTL Q+VLP+FYDVDPS VR Q G FEEAF RH+QR   D D+V+RWRMAL+EAAN
Sbjct: 98  MECRRTLRQLVLPIFYDVDPSTVRKQMGGFEEAFLRHEQRFFTDIDRVLRWRMALSEAAN 157

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRT-LHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G++L+N A GHE +FIR IVEK+SR+LN T L +A Y VGIES V  + S+LD+   G
Sbjct: 158 LSGWDLRNIADGHEAKFIRLIVEKISRELNSTYLFIALYPVGIESRVKVVASHLDIGSNG 217

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFE----PVRLQEKLFKALL 180
           VR +GI GMGG+GKTTIAKA+YNQLY +FE  CFL NIKD  +     +RLQE+L  ++ 
Sbjct: 218 VRFVGILGMGGMGKTTIAKALYNQLYHNFEAKCFLANIKDISKQPNGQIRLQEQLLSSVT 277

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KS++I+L+NVD GI +LQ+RLR KKVLL+LDD+D++ QL A+ RSREWFG GSRI++TTR
Sbjct: 278 KSTKIKLENVDRGIVVLQERLRCKKVLLILDDVDEIGQLNAIARSREWFGPGSRIVLTTR 337

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           D+ LL  L+ D I +V+EMD+TEAL+LFS +AF  + P + +++LSK VV Y GGLPLAL
Sbjct: 338 DQHLLNQLEADGICSVDEMDDTEALELFSWHAFRNSYPLETFHELSKRVVNYSGGLPLAL 397

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L GR   EWE  LNKLK IP   IQ KL+IS+DGL D T+K+IFLDVSCFFIGM
Sbjct: 398 EVLGSFLFGRSRAEWEVTLNKLKKIPDDQIQRKLRISFDGLNDHTYKDIFLDVSCFFIGM 457

Query: 361 DKNKVDVT--------------------------NKLMMHDLLRDMGREIVLNESPNEPG 420
           DKN V+                            NKLMMHD LRDMGREIV  + P EP 
Sbjct: 458 DKNYVEQVLDGCGSFPKIGISVLLQRCLLTIGDKNKLMMHDFLRDMGREIVREKFPKEPE 517

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           K SRL  ++EV+ VL + KGT  IEGLSLK+P+ S + L +KAF+ MQ LR LQLN+  +
Sbjct: 518 KHSRLVLHEEVISVLSRHKGTYAIEGLSLKLPRFSKEKLSSKAFNEMQNLRLLQLNFVNL 577

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
            GDFKH  QE+RWLCWHGFPLKFLP++F++EK+V +DL YS +  FF K+ KF L+NL  
Sbjct: 578 TGDFKHISQEIRWLCWHGFPLKFLPKDFHMEKLVAVDLRYSQI-RFFWKESKF-LKNLKF 637

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHSHYL  TP+F K P+LE LKL+ CKSLV++  +IG LK L+ I+L  C CL +LP
Sbjct: 638 LNLSHSHYLTYTPDFSKLPNLETLKLKDCKSLVELHPTIGELKALILINLKDCKCLKSLP 697

Query: 601 ----------------------------ETLSLKFLYADDTCIQELPRTIVELKKLDCLS 660
                                       E +SL  L ADDT IQ +P TIV+LKKL  LS
Sbjct: 698 KGFSMLKSLETLILSGCSKLSTLPEDLGEMVSLITLTADDTAIQRIPSTIVKLKKLKYLS 757

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 717
           +  CKGPPSKS     W WI P +   S +LP+SL+GL SL  L L +CNLS++ IPKDI
Sbjct: 758 LCGCKGPPSKSFPSAFWSWISPTKYPNSSLLPSSLQGLNSLRTLRLKNCNLSNNTIPKDI 817

BLAST of Lcy12g002930 vs. NCBI nr
Match: XP_022155220.1 (TMV resistance protein N-like isoform X1 [Momordica charantia])

HSP 1 Score: 928.3 bits (2398), Expect = 4.0e-266
Identity = 495/768 (64.45%), Postives = 563/768 (73.31%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDN-DKVVRWRMALTEAA 60
           MEC+RTLNQ+VLP+FYDVDPSDVRNQ GSF EAFSR+Q     +N +K++ WRMALTEAA
Sbjct: 92  MECRRTLNQLVLPVFYDVDPSDVRNQKGSFGEAFSRYQLHFGSENYNKILSWRMALTEAA 151

Query: 61  NLAGFELKNTAHGHEGEFIREIVEKVSRDLNRTLHVAHYQVGIESHVDAITSNLDVELGG 120
           NLAGF L+N A GHEG+FI++I++KVSRDL R + VAH+ VGI+ HV+AITS L V LGG
Sbjct: 152 NLAGFNLENIADGHEGKFIKKIIQKVSRDLKRIIDVAHHLVGIDFHVNAITSKLGVGLGG 211

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALL 180
           VRM+GIWGMGGVGKTTIAKAVYN LY  F+GACFL NIKDT E     V LQEKL K++ 
Sbjct: 212 VRMVGIWGMGGVGKTTIAKAVYNLLYDDFDGACFLDNIKDTSEQLNGLVHLQEKLLKSIF 271

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KSS+IELQ V EGI  LQQRL HKKVLL+LDDIDDM QL AL RSR WFG G+RIIITTR
Sbjct: 272 KSSKIELQYVHEGITQLQQRLPHKKVLLILDDIDDMGQLNALARSRCWFGAGTRIIITTR 331

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           +E  LKN  VDAI T++EM+E EAL+LFS +AFH   PS DY QLSK VV YCGGLPLAL
Sbjct: 332 NEHFLKNFQVDAICTIDEMNENEALELFSWHAFHNKYPSVDYCQLSKRVVSYCGGLPLAL 391

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L+GR V EWEDALNKLK IP  +I  KLK S+DGL DQ +K+IFLD+SCFFIGM
Sbjct: 392 EVLGSFLYGRTVLEWEDALNKLKKIPLDEISRKLKTSFDGLGDQAYKDIFLDISCFFIGM 451

Query: 361 DKN--------------------------KVDVTNKLMMHDLLRDMGREIVLNESPNEPG 420
           DK+                          +VD  NK  MHDLLRDMGREIV NES  EP 
Sbjct: 452 DKSEVTRILDGCGFFPIIGISVLLQRRLLRVDHENKFRMHDLLRDMGREIVHNESRKEPA 511

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           KRSR+F ND+VL VLEKQKG DV EGLSLK+  SS K  HTKAFS MQKLR LQLNYA I
Sbjct: 512 KRSRIFVNDDVLDVLEKQKGADVTEGLSLKLSISSKKNFHTKAFSEMQKLRLLQLNYARI 571

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
           +GDFKH  QELRW+CWHGFPL+ LP+ F+LEK V IDL YSHL  FF+K+ KFSLE LT+
Sbjct: 572 RGDFKHISQELRWICWHGFPLESLPKSFHLEKAVAIDLRYSHLIKFFQKEPKFSLEKLTI 631

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHS YL CTPNF K PHL++L+LE CKSLVKVDDSIG L+KL +I+L  CICLN LP
Sbjct: 632 LNLSHSRYLTCTPNFSKLPHLQKLELEDCKSLVKVDDSIGCLQKLGFINLKDCICLNKLP 691

Query: 601 ETL----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLS 660
           E                              SL  L AD TCIQELP TIV LKKL CLS
Sbjct: 692 EAFCELKSLERLILSGCSKLEKLPNELGKMESLTTLAADGTCIQELPSTIVGLKKLKCLS 751

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 710
           ++R     SKS  EI W  IFP + S SIVLP  L+G +SLT L L +CN+SDDAIPKDI
Sbjct: 752 ITRSNRLLSKSLAEILWSRIFPSKFSNSIVLPMCLQGFKSLTILRLRNCNVSDDAIPKDI 811

BLAST of Lcy12g002930 vs. NCBI nr
Match: XP_022155221.1 (TMV resistance protein N-like isoform X2 [Momordica charantia])

HSP 1 Score: 928.3 bits (2398), Expect = 4.0e-266
Identity = 495/768 (64.45%), Postives = 563/768 (73.31%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDN-DKVVRWRMALTEAA 60
           MEC+RTLNQ+VLP+FYDVDPSDVRNQ GSF EAFSR+Q     +N +K++ WRMALTEAA
Sbjct: 92  MECRRTLNQLVLPVFYDVDPSDVRNQKGSFGEAFSRYQLHFGSENYNKILSWRMALTEAA 151

Query: 61  NLAGFELKNTAHGHEGEFIREIVEKVSRDLNRTLHVAHYQVGIESHVDAITSNLDVELGG 120
           NLAGF L+N A GHEG+FI++I++KVSRDL R + VAH+ VGI+ HV+AITS L V LGG
Sbjct: 152 NLAGFNLENIADGHEGKFIKKIIQKVSRDLKRIIDVAHHLVGIDFHVNAITSKLGVGLGG 211

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALL 180
           VRM+GIWGMGGVGKTTIAKAVYN LY  F+GACFL NIKDT E     V LQEKL K++ 
Sbjct: 212 VRMVGIWGMGGVGKTTIAKAVYNLLYDDFDGACFLDNIKDTSEQLNGLVHLQEKLLKSIF 271

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KSS+IELQ V EGI  LQQRL HKKVLL+LDDIDDM QL AL RSR WFG G+RIIITTR
Sbjct: 272 KSSKIELQYVHEGITQLQQRLPHKKVLLILDDIDDMGQLNALARSRCWFGAGTRIIITTR 331

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           +E  LKN  VDAI T++EM+E EAL+LFS +AFH   PS DY QLSK VV YCGGLPLAL
Sbjct: 332 NEHFLKNFQVDAICTIDEMNENEALELFSWHAFHNKYPSVDYCQLSKRVVSYCGGLPLAL 391

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L+GR V EWEDALNKLK IP  +I  KLK S+DGL DQ +K+IFLD+SCFFIGM
Sbjct: 392 EVLGSFLYGRTVLEWEDALNKLKKIPLDEISRKLKTSFDGLGDQAYKDIFLDISCFFIGM 451

Query: 361 DKN--------------------------KVDVTNKLMMHDLLRDMGREIVLNESPNEPG 420
           DK+                          +VD  NK  MHDLLRDMGREIV NES  EP 
Sbjct: 452 DKSEVTRILDGCGFFPIIGISVLLQRRLLRVDHENKFRMHDLLRDMGREIVHNESRKEPA 511

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           KRSR+F ND+VL VLEKQKG DV EGLSLK+  SS K  HTKAFS MQKLR LQLNYA I
Sbjct: 512 KRSRIFVNDDVLDVLEKQKGADVTEGLSLKLSISSKKNFHTKAFSEMQKLRLLQLNYARI 571

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
           +GDFKH  QELRW+CWHGFPL+ LP+ F+LEK V IDL YSHL  FF+K+ KFSLE LT+
Sbjct: 572 RGDFKHISQELRWICWHGFPLESLPKSFHLEKAVAIDLRYSHLIKFFQKEPKFSLEKLTI 631

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHS YL CTPNF K PHL++L+LE CKSLVKVDDSIG L+KL +I+L  CICLN LP
Sbjct: 632 LNLSHSRYLTCTPNFSKLPHLQKLELEDCKSLVKVDDSIGCLQKLGFINLKDCICLNKLP 691

Query: 601 ETL----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLS 660
           E                              SL  L AD TCIQELP TIV LKKL CLS
Sbjct: 692 EAFCELKSLERLILSGCSKLEKLPNELGKMESLTTLAADGTCIQELPSTIVGLKKLKCLS 751

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 710
           ++R     SKS  EI W  IFP + S SIVLP  L+G +SLT L L +CN+SDDAIPKDI
Sbjct: 752 ITRSNRLLSKSLAEILWSRIFPSKFSNSIVLPMCLQGFKSLTILRLRNCNVSDDAIPKDI 811

BLAST of Lcy12g002930 vs. NCBI nr
Match: XP_022155243.1 (TMV resistance protein N [Momordica charantia])

HSP 1 Score: 848.6 bits (2191), Expect = 4.0e-242
Identity = 449/776 (57.86%), Postives = 557/776 (71.78%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC+RTL Q+VLP+FYDVDPS VR Q   FE AF RH++R   D D+V+RWRMALTEAAN
Sbjct: 93  MECRRTLRQLVLPVFYDVDPSTVRKQTDGFEHAFLRHEERFFFDIDRVLRWRMALTEAAN 152

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRT-LHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G++L+N A GHE +FIR IV+K+SR+LN T L+VA Y VGIES    ITS L+    G
Sbjct: 153 LSGWDLRNVADGHEAKFIRLIVDKISRELNSTYLYVALYPVGIESRTKVITSQLEKGSDG 212

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFE----PVRLQEKLFKALL 180
           VRM+GIWGMGG GKTTIAKAVYNQLY +FEG CFL NIK+        VRLQE+L  ++ 
Sbjct: 213 VRMVGIWGMGGTGKTTIAKAVYNQLYHNFEGRCFLANIKEISNQPDGQVRLQEQLLCSIS 272

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KSS+ +L+NVD GI LLQ+R R KKVLL+LDD+D + QL A+ RSREWFG+GSRI+ITTR
Sbjct: 273 KSSKTKLENVDRGIVLLQERFRRKKVLLILDDVDQIGQLNAIARSREWFGSGSRIVITTR 332

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           D+ LL+ L VDAI +V+EM++TEAL+LFS +AF  + P +D+++LSK VV Y GGLPLAL
Sbjct: 333 DKHLLEQLRVDAICSVDEMNDTEALELFSWHAFRNSYPDEDFHELSKCVVDYSGGLPLAL 392

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L GR + EW+DALNKLKTIP   IQ KL+IS+DGL D T+K+IFLDVSCFFIGM
Sbjct: 393 EVLGSFLFGRSIPEWKDALNKLKTIPDDQIQRKLRISFDGLSDHTYKDIFLDVSCFFIGM 452

Query: 361 DKNKVDVT--------------------------NKLMMHDLLRDMGREIVLNESPNEPG 420
           D+N V+                            NKLMMHDLLRDMGREIV  + P EP 
Sbjct: 453 DRNYVEQVLDGCGFFPKIGISVLLQRCLLTIGDRNKLMMHDLLRDMGREIVREKHPKEPE 512

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           + SRL  +DEVL VL +QKGTD IEGL+LK+P+ S + L T+AF+ MQ LR LQLN+  +
Sbjct: 513 RHSRLLLHDEVLSVLTRQKGTDAIEGLALKLPRFSKEKLSTEAFNEMQNLRLLQLNFVNL 572

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
            GDFKH  QELRWLCWHGFPLKFLP++F++EK+V IDL YSH+  FF K+ KF LE L +
Sbjct: 573 NGDFKHLSQELRWLCWHGFPLKFLPKDFHIEKLVAIDLRYSHI-RFFWKESKF-LEKLKI 632

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHSH L  TP+FLK P LE+LKL+ CK+LV++  SIG LK LV+I+L  C  L +LP
Sbjct: 633 LNLSHSHDLTHTPDFLKLPSLEKLKLKDCKNLVELHHSIGELKGLVFINLKDCKRLKSLP 692

Query: 601 ETL----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLS 660
           E+                             SL  L ADDT IQ++P TIV+LK L  LS
Sbjct: 693 ESFSKLKSLETLTISGCSKINTLPEDLGELKSLITLIADDTAIQQVPSTIVKLKNLKYLS 752

Query: 661 VSRCKGPPSKSHVEISWPWIFP-RQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKD 717
           +  CKGPPSKS   + W WI P +Q   SIVLP SL+GL SL  L L++CNLS++ IPKD
Sbjct: 753 LCGCKGPPSKSLPSLFWSWISPKKQHPNSIVLPASLQGLNSLRTLRLNNCNLSNNTIPKD 812

BLAST of Lcy12g002930 vs. NCBI nr
Match: XP_022934694.1 (TMV resistance protein N [Cucurbita moschata])

HSP 1 Score: 840.1 bits (2169), Expect = 1.4e-239
Identity = 436/775 (56.26%), Postives = 552/775 (71.23%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC+RTL Q+VLP+FYDVDPS VR Q G FEEAF RH+QR   D D+V+RWRMAL+EAAN
Sbjct: 98  MECRRTLRQLVLPIFYDVDPSTVRKQTGGFEEAFLRHEQRFFTDIDRVLRWRMALSEAAN 157

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRT-LHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G++L+N A GHE +F+R IVEK+SR+LN T L +A Y VGIES V  + S+LD+   G
Sbjct: 158 LSGWDLRNIADGHEAKFVRLIVEKISRELNSTYLFIALYPVGIESRVKVVASHLDIGSNG 217

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFE----PVRLQEKLFKALL 180
           VR +GI GMGG+GKTTIAKA+YNQLY +FE  CFL NIKD  +     +RLQE+L  +L 
Sbjct: 218 VRFVGILGMGGMGKTTIAKALYNQLYHNFEAKCFLANIKDISKQPSGQIRLQEQLLSSLT 277

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KS++I+L+NVD GI +LQ+RLR KKVLL+LDD+D++ QL A+ RSREWFG+GSRI++TTR
Sbjct: 278 KSTKIKLENVDRGIVVLQERLRRKKVLLILDDVDEIGQLNAIARSREWFGSGSRIVLTTR 337

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           D+ LL  L+VD I +V+EMD+TEAL+LFS +AF  + PS+ +++LSK VV Y GGLPLAL
Sbjct: 338 DQHLLNQLEVDGICSVDEMDDTEALELFSWHAFRNSYPSETFHELSKRVVNYSGGLPLAL 397

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L GR   EWED LNKLKTIP   IQ KL+IS+DGL D T+K+IFLDVSCFFIG+
Sbjct: 398 EVLGSFLFGRSRAEWEDTLNKLKTIPDDQIQRKLRISFDGLNDHTYKDIFLDVSCFFIGV 457

Query: 361 DKNKVDVT--------------------------NKLMMHDLLRDMGREIVLNESPNEPG 420
           DKN V+                            NKLMMHDLLRDMGREIV  + P EP 
Sbjct: 458 DKNYVEQVLDGCGFFPKIGISVLLQRCLLTIGDKNKLMMHDLLRDMGREIVREKFPKEPE 517

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           + SRL  ++EV+ VL + KGT  IEGLSLK+P+ S + L +KAF+ MQ LR LQLN+  +
Sbjct: 518 RHSRLVLHEEVISVLSRHKGTYAIEGLSLKLPRFSKEKLSSKAFNEMQNLRLLQLNFVTL 577

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
            GDFKH  QE+RWLCWHGFPLKFLP++F++EK+V +DL +S +  FF K+ KF L+NL  
Sbjct: 578 TGDFKHISQEIRWLCWHGFPLKFLPKDFHMEKLVAVDLRHSQI-RFFWKESKF-LKNLKF 637

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHSHYL  TP+F K P+LE LKL+ CKSLV++  +IG LK+L+ I+L  C CL +LP
Sbjct: 638 LNLSHSHYLTYTPDFSKLPNLETLKLKDCKSLVELHPTIGELKRLILINLKDCKCLKSLP 697

Query: 601 ----------------------------ETLSLKFLYADDTCIQELPRTIVELKKLDCLS 660
                                       E +SL  L ADDT IQ +P TIV+LK L  LS
Sbjct: 698 KGFSMLKSLETLFLSGCSKLSTLPEDLGEMVSLITLTADDTAIQRIPSTIVKLKNLKYLS 757

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 717
           +  CKGPPSKS     W WI P +   SI+LP+SL+GL +L  L L++CNLS++ IPKDI
Sbjct: 758 LCGCKGPPSKSFPSAFWSWILPTKYPNSILLPSSLQGLNALRTLRLNNCNLSNNTIPKDI 817

BLAST of Lcy12g002930 vs. NCBI nr
Match: XP_023528161.1 (TMV resistance protein N [Cucurbita pepo subsp. pepo])

HSP 1 Score: 839.0 bits (2166), Expect = 3.2e-239
Identity = 438/775 (56.52%), Postives = 550/775 (70.97%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC+RTL Q+VLP+FYDVDPS VR Q G FEEAF RH+QR   D D+V+RWRMAL+EAAN
Sbjct: 98  MECRRTLRQLVLPIFYDVDPSTVRKQTGGFEEAFLRHEQRFFTDIDRVLRWRMALSEAAN 157

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDLNRT-LHVAHYQVGIESHVDAITSNLDVELGG 120
           L+G++L+N A GHE +FIR IVEK+SR+LN T L +A Y VGIES V  + S+LD+   G
Sbjct: 158 LSGWDLRNIADGHEAKFIRLIVEKISRELNSTYLFIALYPVGIESRVKVVASHLDIGSNG 217

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFE----PVRLQEKLFKALL 180
           VR +GI GMGG+GKTTIAKA+YNQLY +FE  CFL NIKD  +     +RLQE+L  +L 
Sbjct: 218 VRFVGILGMGGMGKTTIAKALYNQLYHNFEAKCFLANIKDISKQPNGQIRLQEQLLSSLT 277

Query: 181 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 240
           KS++I+L+NVD GI +LQ+RLR KKVLL+LDD+D++ QL A+ RSREWFG+GSRI++TTR
Sbjct: 278 KSTKIKLENVDRGIVVLQERLRRKKVLLILDDVDEIGQLNAIARSREWFGSGSRIVLTTR 337

Query: 241 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 300
           D+ LL  L+VD I +V+EMD+TEAL+LFS +AF  + PS+ +++LSK VV Y GGLPLAL
Sbjct: 338 DQHLLNQLEVDGICSVDEMDDTEALELFSWHAFRNSYPSETFHELSKRVVNYSGGLPLAL 397

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L GR   EWED LNKLK IP   IQ KL+IS+DGL D T+K+IFLDVSCFFIG+
Sbjct: 398 EVLGSFLFGRSRAEWEDTLNKLKKIPDDQIQRKLRISFDGLNDHTYKDIFLDVSCFFIGV 457

Query: 361 DKNKVDVT--------------------------NKLMMHDLLRDMGREIVLNESPNEPG 420
           DKN V+                            NKLMMHDLLRDMGREIV  + P EP 
Sbjct: 458 DKNYVEQVLDGCGFFPKIGISVLLQRCLLTIGDKNKLMMHDLLRDMGREIVREKFPKEPE 517

Query: 421 KRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGI 480
           + SRL  ++EVL VL + KGT  IEGLSLK+P+ S + L +KAF+ MQ LR LQLN+  +
Sbjct: 518 RHSRLVLHEEVLSVLSRHKGTYAIEGLSLKLPRFSKEKLSSKAFNEMQNLRLLQLNFVNL 577

Query: 481 QGDFKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTV 540
            GDFKH  QE+RWLCWHGFPLKFLP++F++EK+V +DL YS +  FF K+ KF L+NL  
Sbjct: 578 TGDFKHISQEIRWLCWHGFPLKFLPKDFHMEKLVAVDLRYSQI-RFFWKESKF-LKNLKF 637

Query: 541 LNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDLSCCICLNNLP 600
           LNLSHSHYL  TP+F K P+LE LKL+ CKSLV++  +IG LK L+ I+L  C CL +LP
Sbjct: 638 LNLSHSHYLTYTPDFSKLPNLETLKLKDCKSLVELHPTIGELKALILINLKDCKCLKSLP 697

Query: 601 ----------------------------ETLSLKFLYADDTCIQELPRTIVELKKLDCLS 660
                                       E +SL  L ADDT IQ +P TIV+LK L  LS
Sbjct: 698 KGFSMLKSLETLILSGCSKLSTLPEDLGEMVSLITLTADDTAIQRIPSTIVKLKNLKYLS 757

Query: 661 VSRCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDI 717
           +  CKGPPSKS     W WI P +   SI+LP+SL+GL +L  L L++CNLS++ IPKDI
Sbjct: 758 LCGCKGPPSKSFPSAFWSWISPTKYPNSILLPSSLQGLNALRTLRLNNCNLSNNTIPKDI 817

BLAST of Lcy12g002930 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 539.7 bits (1389), Expect = 3.7e-153
Identity = 321/768 (41.80%), Postives = 462/768 (60.16%), Query Frame = 0

Query: 4   KRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAANLAG 63
           K   + +V P+F  VDPSD+R Q GS+ ++FS+H+    L  +K+  WR ALT+ AN++G
Sbjct: 97  KNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPL--NKLKDWREALTKVANISG 156

Query: 64  FELKNTAHGHEGEFIREIVEKVSRDLN-RTLHVAHYQVGIESHVDAITSNLDVELGGVRM 123
           +++KN    +E E I +I  ++ + L  + LHV  Y VG+ S +  I+S L +   GVR+
Sbjct: 157 WDIKNR---NEAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRV 216

Query: 124 LGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALLKSS 183
           + I+GMGG+GKTT+AK  +N+    FEG+ FL N ++  +       LQ +L   +L+ +
Sbjct: 217 IVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRN 276

Query: 184 RIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTRDEQ 243
            IE + +D  +   ++R R K+VLLV+DD+DD+ QL +    R+ FG GSRIIITTR+  
Sbjct: 277 DIEFKGLDHAV---KERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMH 336

Query: 244 LLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLALQVL 303
           LLK L  +  Y+  E+D  E+L+LFS +AF  + P K++ Q S+ VV YC GLPLA++VL
Sbjct: 337 LLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVL 396

Query: 304 GSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGMDKN 363
           G+ L  R +REWE  L  LK IP+ +IQ KL+IS++ L  +  K++FLD++CFFIG+D  
Sbjct: 397 GAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIE-QKDVFLDIACFFIGVDSY 456

Query: 364 KV-----------DVT--------------NKLMMHDLLRDMGREIVLNESPNEPGKRSR 423
            V           D+               N +MMHDLLRDMGR+IV   SP + G+RSR
Sbjct: 457 YVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSR 516

Query: 424 LFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGIQGDF 483
           L+ +++V+ VL+K+ GT+ IEGLSLK      +    +AF+ MQ+LR L+L Y  + G +
Sbjct: 517 LWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSY 576

Query: 484 KHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLT-VLNL 543
           +HFP++LRWLCWHGF L+  P   +LE +  +DL YS+L  F++ +      N+   L+L
Sbjct: 577 EHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDL 636

Query: 544 SHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSL-KKLVYIDLSCCICLNNLPET 603
           SHS YL+ TP+F   P++E+L L  CKSLV V  SIG L KKLV ++LS CI L+ LPE 
Sbjct: 637 SHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEE 696

Query: 604 L----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLSVS 663
           +                            SL  L AD T ++E+P TI +LKKL  LS++
Sbjct: 697 IYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLN 756

Query: 664 RCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDIGS 712
            CKG  S     +       +  S+S++ P SL GL  +  L+L +CNLSD+ IP+DIGS
Sbjct: 757 GCKGLLSDDIDNL----YSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGS 816

BLAST of Lcy12g002930 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 539.7 bits (1389), Expect = 3.7e-153
Identity = 321/768 (41.80%), Postives = 462/768 (60.16%), Query Frame = 0

Query: 4   KRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAANLAG 63
           K   + +V P+F  VDPSD+R Q GS+ ++FS+H+    L  +K+  WR ALT+ AN++G
Sbjct: 100 KNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPL--NKLKDWREALTKVANISG 159

Query: 64  FELKNTAHGHEGEFIREIVEKVSRDLN-RTLHVAHYQVGIESHVDAITSNLDVELGGVRM 123
           +++KN    +E E I +I  ++ + L  + LHV  Y VG+ S +  I+S L +   GVR+
Sbjct: 160 WDIKNR---NEAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRV 219

Query: 124 LGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALLKSS 183
           + I+GMGG+GKTT+AK  +N+    FEG+ FL N ++  +       LQ +L   +L+ +
Sbjct: 220 IVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRN 279

Query: 184 RIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTRDEQ 243
            IE + +D  +   ++R R K+VLLV+DD+DD+ QL +    R+ FG GSRIIITTR+  
Sbjct: 280 DIEFKGLDHAV---KERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMH 339

Query: 244 LLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLALQVL 303
           LLK L  +  Y+  E+D  E+L+LFS +AF  + P K++ Q S+ VV YC GLPLA++VL
Sbjct: 340 LLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVL 399

Query: 304 GSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGMDKN 363
           G+ L  R +REWE  L  LK IP+ +IQ KL+IS++ L  +  K++FLD++CFFIG+D  
Sbjct: 400 GAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIE-QKDVFLDIACFFIGVDSY 459

Query: 364 KV-----------DVT--------------NKLMMHDLLRDMGREIVLNESPNEPGKRSR 423
            V           D+               N +MMHDLLRDMGR+IV   SP + G+RSR
Sbjct: 460 YVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSR 519

Query: 424 LFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYAGIQGDF 483
           L+ +++V+ VL+K+ GT+ IEGLSLK      +    +AF+ MQ+LR L+L Y  + G +
Sbjct: 520 LWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSY 579

Query: 484 KHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLT-VLNL 543
           +HFP++LRWLCWHGF L+  P   +LE +  +DL YS+L  F++ +      N+   L+L
Sbjct: 580 EHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDL 639

Query: 544 SHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSL-KKLVYIDLSCCICLNNLPET 603
           SHS YL+ TP+F   P++E+L L  CKSLV V  SIG L KKLV ++LS CI L+ LPE 
Sbjct: 640 SHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEE 699

Query: 604 L----------------------------SLKFLYADDTCIQELPRTIVELKKLDCLSVS 663
           +                            SL  L AD T ++E+P TI +LKKL  LS++
Sbjct: 700 IYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLN 759

Query: 664 RCKGPPSKSHVEISWPWIFPRQVSISIVLPTSLRGLESLTELNLSHCNLSDDAIPKDIGS 712
            CKG  S     +       +  S+S++ P SL GL  +  L+L +CNLSD+ IP+DIGS
Sbjct: 760 GCKGLLSDDIDNL----YSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGS 819

BLAST of Lcy12g002930 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 401.4 bits (1030), Expect = 1.6e-111
Identity = 267/760 (35.13%), Postives = 401/760 (52.76%), Query Frame = 0

Query: 1   MECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAAN 60
           MEC +     ++P+FY+VDPSDVR Q GSF E    H      D +KV +W+ AL + A 
Sbjct: 97  MECNK---DTIVPIFYEVDPSDVRRQRGSFGEDVESHS-----DKEKVGKWKEALKKLAA 156

Query: 61  LAGFELKNTAHGHEGEFIREIVEKVSRDL-NRTLHVAHYQVGIESHVDAITSNLDVELGG 120
           ++G + +N     + + I++IV+ +S  L + +   +   +G+ SH+D + S + +    
Sbjct: 157 ISGEDSRN---WDDSKLIKKIVKDISDKLVSTSWDDSKGLIGMSSHMDFLQSMISIVDKD 216

Query: 121 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDT---FEPVRLQEKLFKALLK 180
           VRMLGIWGMGGVGKTTIAK +YNQL   F+  CF+ N+K+    +   RLQ +    + +
Sbjct: 217 VRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVCNRYGVRRLQVEFLCRMFQ 276

Query: 181 SSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTRD 240
               E  +      ++++R RHK V +VLDD+D  +QL  L +   WFG GSRII+TTRD
Sbjct: 277 ERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRD 336

Query: 241 EQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQN-CPSKDYYQLSKSVVKYCGGLPLAL 300
             LL +  ++ +Y V  + + EALQLF  YAF +       + +LS   V Y  GLPLAL
Sbjct: 337 RHLLLSHGINLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLAL 396

Query: 301 QVLGSSLHGRMVREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGM 360
           +VLGS L+ R   EWE  L +LKT PH DI E L++SYDGL D+  K IFL +SCF+   
Sbjct: 397 RVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGL-DEQEKAIFLYISCFY--- 456

Query: 361 DKNKVDVTNKLM----------------------------MHDLLRDMGREIVLNESPNE 420
           +  +VD   KL+                            +HDLL  MGRE+V  ++ N 
Sbjct: 457 NMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNN 516

Query: 421 PGKRSRLFFNDEVLYVLEKQKGTDVIEGLSLKMPKSSNKVLHTKAFSAMQKLRYLQLNYA 480
           P +R  L+  +++ ++L +  GT ++EG+SL + + S      +AF  +  L+ L     
Sbjct: 517 PAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFYDL 576

Query: 481 GIQGD--------FKHFPQELRWLCWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKK 540
              G+          + P++LR+L W G+PLK +P  F  E +V + +S S+L   +   
Sbjct: 577 SFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGI 636

Query: 541 QKFSLENLTVLNLSHSHYLKCTPNFLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYIDL 600
           Q   L NL  ++LS   YL   P+  K  +LEEL L YC+SLV+V  SI +LK L    L
Sbjct: 637 Q--PLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYL 696

Query: 601 SCCICLNNLPETLSLKFLYADDTCIQELPRTIVELKKLDCLSVSRCKGPPSKSHVEISWP 660
           + CI L ++P                      + LK L+ + +S C     K   EISW 
Sbjct: 697 TNCIQLKDIP--------------------IGIILKSLETVGMSGCSS--LKHFPEISWN 756

Query: 661 WIFPRQVSISIV----LPTSLRGLESLTELNLSHCNLSDDAIPKDIGSLVSLVILDLEG- 715
               R++ +S      LP+S+  L  L +L++S C      +P  +G LVSL  L+L+G 
Sbjct: 757 ---TRRLYLSSTKIEELPSSISRLSCLVKLDMSDCQRL-RTLPSYLGHLVSLKSLNLDGC 813

BLAST of Lcy12g002930 vs. TAIR 10
Match: AT1G27170.1 (transmembrane receptors;ATP binding )

HSP 1 Score: 391.7 bits (1005), Expect = 1.2e-108
Identity = 269/825 (32.61%), Postives = 423/825 (51.27%), Query Frame = 0

Query: 4   KRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAANLAG 63
           K +L++ +LP+FY VDPS VR Q+   ++ F  HQ R   + +KV  WR ALT   NLAG
Sbjct: 95  KSSLDRRILPIFYHVDPSHVRKQSDHIKKDFEEHQVRFSEEKEKVQEWREALTLVGNLAG 154

Query: 64  FELKNTAHGHEGEFIREIVEKVSRDLNRTLH-VAHYQVGIESHVDAITSNLDVE-LGGVR 123
           +     +   + + I  +V++V  +L+ T   V  + VG+ES +  +T  +D E   GV+
Sbjct: 155 YVCDKDS--KDDDMIELVVKRVLAELSNTPEKVGEFIVGLESPLKDLTGLIDTESSSGVQ 214

Query: 124 MLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALLKS 183
           +LG++GMGG+GKTT+AKA YN++  +FE   F+ +I++        V LQ+ L K L + 
Sbjct: 215 VLGLYGMGGIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKELFRL 274

Query: 184 SRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTRDE 243
              E+++V  G+  ++  +  KK+++VLDD+D + Q+ AL     W+G G+ I+ITTRD 
Sbjct: 275 VP-EIEDVSIGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITTRDS 334

Query: 244 QLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLALQV 303
           ++L  L V+  Y V  + E +AL+LFS ++  +  P+K+   LSK +V+  G LPLA++V
Sbjct: 335 EILSKLSVNQQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLAVEV 394

Query: 304 LGSSLHGRM-VREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIGMD 363
            GS L+ +   ++W+  L+KLK     ++Q+ L++S+  L D+  K +FLD++C F+ M+
Sbjct: 395 FGSLLYDKKEEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDE-EKKVFLDIACLFLKME 454

Query: 364 KNKVDV----------------------------TNKLMMHDLLRDMGREIVLNESPNEP 423
             K +V                             + L MHD +RDMGR++VL ES  +P
Sbjct: 455 IKKDEVVIVLKGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESREDP 514

Query: 424 GKRSRLFFNDEVLYVLEKQKGTDVIEGLSL------------------------------ 483
           G RSRL+   E++ VL   KGT  I G+ L                              
Sbjct: 515 GLRSRLWDRGEIMTVLNNMKGTSSIRGIVLDFKKKFARDPTADEIVSRNLRNNPGIYSVF 574

Query: 484 -------------KMPKSSNKVLHTKAFSAMQKLRYLQLNYAGIQGDFKHFPQELRWLCW 543
                        + PKSS   +  ++F+ M KLR LQ+N   ++G+ K  P EL+W+ W
Sbjct: 575 NYLKNKLVRFPAEEKPKSSEITIPVESFAPMTKLRLLQINNVELEGNLKLLPSELKWIQW 634

Query: 544 HGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTVLNLSHSHYLKCTPNFL 603
            G PL+ LP +F   ++ V+DLS S +      + K   ENL V+ L   H L+  P+  
Sbjct: 635 KGCPLENLPPDFLARQLSVLDLSESGIRQVQTLRNKMVDENLKVVILRGCHSLEAIPDLS 694

Query: 604 KTPHLEELKLEYCKSLVKVDDSIGSLKKLVYID------------------------LSC 663
               LE+L  E C  LVKV  S+G+L+KL+++D                        LS 
Sbjct: 695 NHEALEKLVFEQCTLLVKVPKSVGNLRKLIHLDFRRCSKLSEFLVDVSGLKLLEKLFLSG 754

Query: 664 CICLNNLPETL----SLKFLYADDTCIQELPRTIVELKKLDCLSVSRCK--GPPSKSHVE 721
           C  L+ LPE +    SLK L  D T I+ LP +I  L+ L+ LS+  CK    P      
Sbjct: 755 CSDLSVLPENIGAMTSLKELLLDGTAIKNLPESINRLQNLEILSLRGCKIQELPLCIGTL 814

BLAST of Lcy12g002930 vs. TAIR 10
Match: AT1G27170.2 (transmembrane receptors;ATP binding )

HSP 1 Score: 390.2 bits (1001), Expect = 3.6e-108
Identity = 267/827 (32.29%), Postives = 423/827 (51.15%), Query Frame = 0

Query: 2   ECKRTLNQVVLPLFYDVDPSDVRNQNGSFEEAFSRHQQRSVLDNDKVVRWRMALTEAANL 61
           + K +L ++VLP+FY+V+P  +R QNG +E  F  H +R     +K+ RWR AL    N+
Sbjct: 95  DLKSSLGRLVLPIFYEVEPCMLRKQNGPYEMDFEEHSKR--FSEEKIQRWRRALNIIGNI 154

Query: 62  AGFELKNTAHGHEGEFIREIVEKVSRDLNRTLH-VAHYQVGIESHVDAITSNLDVE-LGG 121
            GF     +   + + I  +V++V  +L+ T   V  + VG+ES +  +T  +D E   G
Sbjct: 155 PGFVYSKDS--KDDDMIELVVKRVLAELSNTPEKVGEFIVGLESPLKDLTGLIDTESSSG 214

Query: 122 VRMLGIWGMGGVGKTTIAKAVYNQLYKHFEGACFLGNIKDTFEP----VRLQEKLFKALL 181
           V++LG++GMGG+GKTT+AKA YN++  +FE   F+ +I++        V LQ+ L K L 
Sbjct: 215 VQVLGLYGMGGIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKELF 274

Query: 182 KSSRIELQNVDEGIPLLQQRLRHKKVLLVLDDIDDMKQLQALGRSREWFGTGSRIIITTR 241
           +    E+++V  G+  ++  +  KK+++VLDD+D + Q+ AL     W+G G+ I+ITTR
Sbjct: 275 RLVP-EIEDVSIGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITTR 334

Query: 242 DEQLLKNLDVDAIYTVNEMDETEALQLFSCYAFHQNCPSKDYYQLSKSVVKYCGGLPLAL 301
           D ++L  L V+  Y V  + E +AL+LFS ++  +  P+K+   LSK +V+  G LPLA+
Sbjct: 335 DSEILSKLSVNQQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLAV 394

Query: 302 QVLGSSLHGRM-VREWEDALNKLKTIPHYDIQEKLKISYDGLKDQTHKNIFLDVSCFFIG 361
           +V GS L+ +   ++W+  L+KLK     ++Q+ L++S+  L D+  K +FLD++C F+ 
Sbjct: 395 EVFGSLLYDKKEEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDE-EKKVFLDIACLFLK 454

Query: 362 MDKNKVDV----------------------------TNKLMMHDLLRDMGREIVLNESPN 421
           M+  K +V                             + L MHD +RDMGR++VL ES  
Sbjct: 455 MEIKKDEVVIVLKGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESRE 514

Query: 422 EPGKRSRLFFNDEVLYVLEKQKGTDVIEGLSL---------------------------- 481
           +PG RSRL+   E++ VL   KGT  I G+ L                            
Sbjct: 515 DPGLRSRLWDRGEIMTVLNNMKGTSSIRGIVLDFKKKFARDPTADEIVSRNLRNNPGIYS 574

Query: 482 ---------------KMPKSSNKVLHTKAFSAMQKLRYLQLNYAGIQGDFKHFPQELRWL 541
                          + PKSS   +  ++F+ M KLR LQ+N   ++G+ K  P EL+W+
Sbjct: 575 VFNYLKNKLVRFPAEEKPKSSEITIPVESFAPMTKLRLLQINNVELEGNLKLLPSELKWI 634

Query: 542 CWHGFPLKFLPEEFNLEKVVVIDLSYSHLTNFFRKKQKFSLENLTVLNLSHSHYLKCTPN 601
            W G PL+ LP +F   ++ V+DLS S +      + K   ENL V+ L   H L+  P+
Sbjct: 635 QWKGCPLENLPPDFLARQLSVLDLSESGIRQVQTLRNKMVDENLKVVILRGCHSLEAIPD 694

Query: 602 FLKTPHLEELKLEYCKSLVKVDDSIGSLKKLVYID------------------------L 661
                 LE+L  E C  LVKV  S+G+L+KL+++D                        L
Sbjct: 695 LSNHEALEKLVFEQCTLLVKVPKSVGNLRKLIHLDFRRCSKLSEFLVDVSGLKLLEKLFL 754

Query: 662 SCCICLNNLPETL----SLKFLYADDTCIQELPRTIVELKKLDCLSVSRCK--GPPSKSH 721
           S C  L+ LPE +    SLK L  D T I+ LP +I  L+ L+ LS+  CK    P    
Sbjct: 755 SGCSDLSVLPENIGAMTSLKELLLDGTAIKNLPESINRLQNLEILSLRGCKIQELPLCIG 814