Lcy09g014270 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g014270
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr09: 16429022 .. 16430004 (-)
RNA-Seq ExpressionLcy09g014270
SyntenyLcy09g014270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTATGCAGGGAGATCCCTACATACCGGTATGCAGCCAAGTTTTTTCCTATTTATATATAGTTAAGGAAAAAACTTGGCTGCATACCGGTGGGTAGTGGGTAGGGATCTCCCTGCATACCCCATTAAAATCTTGACAACTAAGAAAGAAGAAAGTAAAAAGTGATAACTTTTTTTTGTATCTCTCTTAGTTGTCAAGATTTTAATGGTGGTATGTAGGGAGATTCCTACCCACCGGTATGTAGCCAAGTTTTTTCCTATAGTTAATTGATGTGGAGAAGGATAAAACAACCATGGAATATGGAAAGAGTGTAATAAAATATATAGAATGATCATTTATATAATTTCTTTCGAAATGTAGTCGTTTCTTAATAAAGAATCACGTAGTTGAATATTAATAGAGGGAGTAAATATTATAATATAATATATTGAAATTTTTTCTCGGGGATTGCCTACTGCCGCAATCGAGTGATTGGGAGGAGCGCTGTGTATGGAGTCCGCCTCCTGCTAGGAAGCTGAAACTTAATGTTGATGCTTCTGTCAGGCCTGATACAGGGGAAGCGGGGGATGGTTGTGTGCTGCGTGGGGCTGATGGTGAGGTATTTATGGCCGCCTGTTTGGAACTACAGAGGTGTTGGAGCGTGGATTTGGCTGAGGGATGGACTGTGTATAAAGAGGTCCAACTTGCTCGGCAGTTGGGGTTTGCGGAGTTTGTGGTAGAGACTGACTCGCTGAGGTTGGTCAAAATCCTCCATGGCGATCTGCATGATGTTTCAGAAGTGGGGCTGCTAATGGATGACATTCGAGGGATTCTCCACCCTTGGGACAACGGTAAGGTTTTGTTTACATTACGCCAGGGAAACAAGGTGGCGCATGTTCTGGGTAGTTTGGCCTTTTCATATGTTGACCGTGTTTGGCTTGAAGAGTGGCCCAGCGAAATCTCGGATGTCCTGAGGGGTGATGTTGTTTCAGCTGCGTGA

mRNA sequence

ATGGTGGTATGCAGGGAGATCCCTACATACCGGCCTGATACAGGGGAAGCGGGGGATGGTTGTGTGCTGCGTGGGGCTGATGGTGAGGTATTTATGGCCGCCTGTTTGGAACTACAGAGGTGTTGGAGCGTGGATTTGGCTGAGGGATGGACTGTGTATAAAGAGGTCCAACTTGCTCGGCAGTTGGGGTTTGCGGAGTTTGTGGTAGAGACTGACTCGCTGAGGTTGGTCAAAATCCTCCATGGCGATCTGCATGATGTTTCAGAAGTGGGGCTGCTAATGGATGACATTCGAGGGATTCTCCACCCTTGGGACAACGGTAAGGTTTTGTTTACATTACGCCAGGGAAACAAGGTGGCGCATGTTCTGGGTAGTTTGGCCTTTTCATATGTTGACCGTGTTTGGCTTGAAGAGTGGCCCAGCGAAATCTCGGATGTCCTGAGGGGTGATGTTGTTTCAGCTGCGTGA

Coding sequence (CDS)

ATGGTGGTATGCAGGGAGATCCCTACATACCGGCCTGATACAGGGGAAGCGGGGGATGGTTGTGTGCTGCGTGGGGCTGATGGTGAGGTATTTATGGCCGCCTGTTTGGAACTACAGAGGTGTTGGAGCGTGGATTTGGCTGAGGGATGGACTGTGTATAAAGAGGTCCAACTTGCTCGGCAGTTGGGGTTTGCGGAGTTTGTGGTAGAGACTGACTCGCTGAGGTTGGTCAAAATCCTCCATGGCGATCTGCATGATGTTTCAGAAGTGGGGCTGCTAATGGATGACATTCGAGGGATTCTCCACCCTTGGGACAACGGTAAGGTTTTGTTTACATTACGCCAGGGAAACAAGGTGGCGCATGTTCTGGGTAGTTTGGCCTTTTCATATGTTGACCGTGTTTGGCTTGAAGAGTGGCCCAGCGAAATCTCGGATGTCCTGAGGGGTGATGTTGTTTCAGCTGCGTGA

Protein sequence

MVVCREIPTYRPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVETDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSYVDRVWLEEWPSEISDVLRGDVVSAA
Homology
BLAST of Lcy09g014270 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.9e-18
Identity = 58/143 (40.56%), Postives = 81/143 (56.64%), Query Frame = 0

Query: 10  YRPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVV 69
           +R ++  AG G ++R + G V++ A   L R   VD  EG+ VY+ + LA + GF  F +
Sbjct: 120 FRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQI 179

Query: 70  ETDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPW-DNGKVLFTLRQGNKVAHVLGSLAF 129
           ETDSLR+  +L  D  D SEVG+L   I+  L    +     FT R GN  AH+L  LA 
Sbjct: 180 ETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLAL 239

Query: 130 SYVD-RVWLEEWPSEISDVLRGD 151
           +    ++W+EEWP EIS VL  D
Sbjct: 240 TSPHLQIWVEEWPDEISSVLAVD 262

BLAST of Lcy09g014270 vs. ExPASy TrEMBL
Match: A0A5C7ITL2 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001058 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.4e-12
Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 0

Query: 11  RPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVE 70
           R   G +G GCV+R +DG V  + C     C+   +AE   +    +LA +LG    V+E
Sbjct: 72  RDRDGRSGVGCVIRDSDGYVRASLCQGFDVCYPPQVAETLAILHGCRLAAELGLLPTVLE 131

Query: 71  TDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSY 130
           +D+L +V+ +      +SEVG+++ DI  I   +    + F  R  NKV H L  LA  +
Sbjct: 132 SDALSVVEAVRLKSMPLSEVGVVIQDILEIFTSFPVFSICFVPRLANKVVHGLARLAVGH 191

Query: 131 VDR-VWLEEWPSEISDVLRGD 151
           V R VW+ + P  +  ++ GD
Sbjct: 192 VGRFVWVNDCPLAVESLVLGD 212

BLAST of Lcy09g014270 vs. ExPASy TrEMBL
Match: A0A6P9ETQ1 (uncharacterized protein LOC108983473 OS=Juglans regia OX=51240 GN=LOC108983473 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 9.0e-12
Identity = 42/138 (30.43%), Postives = 74/138 (53.62%), Query Frame = 0

Query: 14  TGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVETDS 73
           + + G G V+R   GEV  A     +   +  +AEG  +++ ++L  ++G  +   E DS
Sbjct: 179 SSKIGMGVVIRDKKGEVVAALSASRREGQNAFVAEGMALWRAMELCLEIGMVDVEFEGDS 238

Query: 74  LRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSYVDR 133
             L++ +  D  + S  G +++DI+ + H ++N KV+F  R+ N+VAH L  +A    + 
Sbjct: 239 KELIEAVMSDEEEDSRWGHIVEDIKQLRHQYNNWKVVFNYRESNEVAHNLAKMALLIDEE 298

Query: 134 -VWLEEWPSEISDVLRGD 151
            VW+EE PS I   +  D
Sbjct: 299 LVWIEEVPSSIHQYIVKD 316

BLAST of Lcy09g014270 vs. ExPASy TrEMBL
Match: A0A803MUM1 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-11
Identity = 47/143 (32.87%), Postives = 74/143 (51.75%), Query Frame = 0

Query: 15  GEAGD--GCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVETD 74
           GEAG   G V+R   GEV  A C ++ + W   + E   +   ++LA Q      +VE+D
Sbjct: 180 GEAGSDMGVVVRDHLGEVVRAGCQQVHQSWDASIVEAKAMVLGLKLAMQCNVKRVIVESD 239

Query: 75  SLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLA-FSYV 134
             RLVK+++G   D S +GLL  +I+ +   +D+       R+ N VAH +  L+   Y 
Sbjct: 240 CERLVKLVNGVGKDGSFLGLLAKEIKCVAEFFDSIVFAHVFREANSVAHAMAHLSPLEYS 299

Query: 135 DRVWLEEWPSEISDVLRGDVVSA 155
            RVW+   PS + DV+  + + A
Sbjct: 300 TRVWVWCCPSMVDDVVALEFIPA 322

BLAST of Lcy09g014270 vs. ExPASy TrEMBL
Match: A0A1R3HHY9 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_19197 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-11
Identity = 44/143 (30.77%), Postives = 73/143 (51.05%), Query Frame = 0

Query: 9   TYRPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFV 68
           +++  T   G G V R   GEV  +    L++      AE     K +  AR++GF + +
Sbjct: 724 SFQAATSLGGFGAVGRDGSGEVLGSIAGRLEQVADAFAAEAKAAVKAIIWAREMGFTDII 783

Query: 69  VETDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAF 128
           VE D+L ++K ++  L D+S +G+  ++++     +++       R GN VAH L +   
Sbjct: 784 VEGDALIIIKKVNSTLEDLSPIGIYTEELKFYRSMFNSCSFHHVGRDGNGVAHALATYGI 843

Query: 129 SYV-DRVWLEEWPSEISDVLRGD 151
           S   D VW+EE P  I DVL+ D
Sbjct: 844 SLTEDLVWMEEVPELILDVLKND 866

BLAST of Lcy09g014270 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 101.7 bits (252), Expect = 5.9e-18
Identity = 58/143 (40.56%), Postives = 81/143 (56.64%), Query Frame = 0

Query: 10  YRPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVV 69
           +R ++  AG G ++R + G V++ A   L R   VD  EG+ VY+ + LA + GF  F +
Sbjct: 120 FRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQI 179

Query: 70  ETDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPW-DNGKVLFTLRQGNKVAHVLGSLAF 129
           ETDSLR+  +L  D  D SEVG+L   I+  L    +     FT R GN  AH+L  LA 
Sbjct: 180 ETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLAL 239

Query: 130 SYVD-RVWLEEWPSEISDVLRGD 151
           +    ++W+EEWP EIS VL  D
Sbjct: 240 TSPHLQIWVEEWPDEISSVLAVD 262

BLAST of Lcy09g014270 vs. NCBI nr
Match: XP_030970599.1 (uncharacterized protein LOC115990979 [Quercus lobata])

HSP 1 Score: 88.2 bits (217), Expect = 6.8e-14
Identity = 53/142 (37.32%), Postives = 76/142 (53.52%), Query Frame = 0

Query: 13  DTGEAGDGCVLRGADGEVFMAACLE-LQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVET 72
           +   +G G V+R  DG V MA+C E L   +  +  E     K +QLA  LGF   ++E 
Sbjct: 88  NANSSGVGAVIRNHDGAV-MASCAEKLNEAYKAEEIEALAALKALQLAFDLGFQNAILEG 147

Query: 73  DSLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSYV 132
           DSL L+K L  D H++S +GLL++D++     + +       R GN VAH L   A S  
Sbjct: 148 DSLGLIKALKADDHNLSPLGLLVEDVKLAASNFVSLSYSHIKRNGNSVAHNLAKHAISIP 207

Query: 133 D-RVWLEEWPSEISDVLRGDVV 153
           D +VW+E+ PS +   L  DVV
Sbjct: 208 DFQVWMEDVPSHVVSFLHSDVV 228

BLAST of Lcy09g014270 vs. NCBI nr
Match: XP_021716820.1 (uncharacterized protein LOC110684687 [Chenopodium quinoa])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 50/143 (34.97%), Postives = 77/143 (53.85%), Query Frame = 0

Query: 13  DTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVETD 72
           D    G G V+R A GEV +A CL L+  + VD+AE   +   +++A +  F +  +ETD
Sbjct: 159 DNSAVGLGGVMRDALGEVVVATCLCLRSKYEVDVAEALALRHSLRIALESEFRKVCLETD 218

Query: 73  SLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSYVD 132
            +++   L       + +G++++DI  +     +    F  R GN  AH L  L+ SY D
Sbjct: 219 CMKVFSHLTKGCAPATILGMVINDIMQLARGCQSCSFSFVKRSGNGAAHALAKLSSSYGD 278

Query: 133 -RVWLEEWPSEISDVLRGDVVSA 155
            RVW+EE PSEIS  +  D+ SA
Sbjct: 279 LRVWMEEVPSEISPFVMADLASA 301

BLAST of Lcy09g014270 vs. NCBI nr
Match: KAF7802400.1 (uncharacterized protein G2W53_041511 [Senna tora])

HSP 1 Score: 85.5 bits (210), Expect = 4.4e-13
Identity = 43/146 (29.45%), Postives = 80/146 (54.79%), Query Frame = 0

Query: 13  DTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVETD 72
           D G+   GC++R   G    A   E+  C+SV++AE   VY+ + +A+++     +VE D
Sbjct: 623 DGGQTRVGCIIRDYMGRCLAAMTKEITGCYSVEMAEALAVYEGMVVAKRMSILNVLVEGD 682

Query: 73  SLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSYVD 132
           S  ++K+L+G+  D + V  ++ DI  +   + +    +  R+ NKVAH+L     ++  
Sbjct: 683 SASVIKLLNGEGMDCTYVSFIIKDILQLCSSFRSYSFNWVRREANKVAHILAHDPVAFPS 742

Query: 133 -----RVWLEEWPSEISDVLRGDVVS 154
                 VWLE++PS I + L+ D+++
Sbjct: 743 DNSGILVWLEDYPSLICNALKSDILT 768

BLAST of Lcy09g014270 vs. NCBI nr
Match: XP_042964529.1 (uncharacterized protein LOC122298728 [Carya illinoinensis])

HSP 1 Score: 84.0 bits (206), Expect = 1.3e-12
Identity = 46/139 (33.09%), Postives = 74/139 (53.24%), Query Frame = 0

Query: 11  RPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQLGFAEFVVE 70
           R D  + G G V+R  + EV  + C  +       + E   + + VQ   +LGF+  V+E
Sbjct: 78  RIDLNKVGIGVVVRDEEREVLASLCKVVDCLHDPSVGEALALKRAVQFCDELGFSNVVLE 137

Query: 71  TDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAHVLGSLAFSY 130
            D+  +VK  +      +  G +M+++RG L   ++ K+ F  R+ N VAH+L   AF+Y
Sbjct: 138 GDAQVIVKAANNREELYTAYGNVMEEVRGFLKMRESWKIQFIHREANNVAHILAKNAFNY 197

Query: 131 -VDRVWLEEWPSEISDVLR 149
             +RVW+EE P EI  VL+
Sbjct: 198 TTERVWIEEGPIEILHVLQ 216

BLAST of Lcy09g014270 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 8.6e-07
Identity = 38/133 (28.57%), Postives = 61/133 (45.86%), Query Frame = 0

Query: 2   VVCREIPTYRPDTGEAGDGCVLRGADGEVFMAACLELQRCWSVDLAEGWTVYKEVQLARQ 61
           V C    T++ +    G G +LR   G V       L R  +V  AE   +   V    +
Sbjct: 145 VKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSR 204

Query: 62  LGFAEFVVETDSLRLVKILHGDLHDVSEVGLLMDDIRGILHPWDNGKVLFTLRQGNKVAH 121
             +   + E+D+  LV +L+ D      +   ++DI+ +LH ++  K  FT R GNKVA 
Sbjct: 205 FNYKRIIFESDAQALVNLLNSD-DFWPTLQPALEDIQQLLHHFEEVKFEFTPRGGNKVAD 264

Query: 122 VLG--SLAFSYVD 133
            +   S++FS  D
Sbjct: 265 RIARESISFSNYD 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DBJ72.9e-1840.56uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A5C7ITL21.4e-1232.62RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001058 ... [more]
A0A6P9ETQ19.0e-1230.43uncharacterized protein LOC108983473 OS=Juglans regia OX=51240 GN=LOC108983473 P... [more]
A0A803MUM11.2e-1132.87Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A1R3HHY91.5e-1130.77Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_19197 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022150944.15.9e-1840.56uncharacterized protein LOC111018973 [Momordica charantia][more]
XP_030970599.16.8e-1437.32uncharacterized protein LOC115990979 [Quercus lobata][more]
XP_021716820.11.2e-1334.97uncharacterized protein LOC110684687 [Chenopodium quinoa][more]
KAF7802400.14.4e-1329.45uncharacterized protein G2W53_041511 [Senna tora][more]
XP_042964529.11.3e-1233.09uncharacterized protein LOC122298728 [Carya illinoinensis][more]
Match NameE-valueIdentityDescription
AT2G34320.18.6e-0728.57Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 10..124
e-value: 7.4E-18
score: 64.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 8..129
e-value: 4.4E-5
score: 25.5
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 13..141
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 10..123
e-value: 2.5189E-12
score: 58.0944

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g014270.1Lcy09g014270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity