Lcy09g006000 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g006000
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionhydrolase family protein / HAD-superfamily protein
LocationChr09: 5204734 .. 5206844 (-)
RNA-Seq ExpressionLcy09g006000
SyntenyLcy09g006000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGAGTATGGTTTCAAGTAAGCTCTTAGCCAGTCTTGGATTAACGTTCGTCCAATGCCTTCAGAATTGTTGGATCACATATGATGAATTTTTTTTATACCTTTACAGAAAAGTTTTCTCCATTGATGAGTATGCTTCCTTCTTTGAGAATATTGATCCTGTATCCCAATACAAGAACTGGACAACCAAGCAAACTTTTGATTTGACTTTCAACCCTGAGAAGTTGATGAGAAGGCAGAGTGTTTTATCTGAAAGAGTGAAGGCAGCTTTTGTTGTCAGTGACCCTGTTGATTGGGGCAGGGATATCCAAGTACTCATTCTCTTCCTTTTATTCTCTGAGTTGTTCCTGAGCTGGTAACTGTATAGTTGGTTGCATCGTTAAATTTAGCTTTTAAGACTATTATATTCCAAAGTTCCATAAGGTCAATTAATTTCATGTCTAGTTATTTATATTTGTCCAGCAACAAATAATTATTTGTCTTCGTTTTGAAATAACTTGGGCTCTTTAATGGTAACATTTGTCTGTCTGTCTATCTGAAACACTGAAGTACTATACTTTGTGGATAATAATTTCTAAATTTTTCTCCAAGGATATACCCATGTTGATTTTCTAAACGATCTCAATTGTGGAAATTTTATACTGCCACCTCCTCAAAAGTTCAGATTCTTAGTTGAAGATTGTATGTCTTTCAATTCATCAATTTGTTTTAACGTTTATGGTAGTTGAAATTGCGAGATGTTAATGCCTTGTGTTACCAAAACAGAATAAGATATTGAACTATTGCGTAGTCTTCATCCCAAAACTTTTAACCGATTTGTTTCCTTGTAAATTTGAAGCAGCCCCTCTTCTTGATGCTGACATCTTGATAATAGCTATTCTTGCAAAAGCTTCGAACTCATTGATGAATGAATTAGGTTCTTTGTGATGTTTTAAGATCTGGAGGCCTTCCAGGACAGGAGGAGAATGGGGATCAAGTGCCTCTATATTTTGCTGCAGATGATTTTGAATATCAGGTTAGTTTTAATGATTCTTTAAGCAATGAATTGCTTACATGGAGAACTAGTTCTTTAGGGTTGTTTTAGTTTACGTCTGCAGTTAGTGCCAGCATAGGGTCACAGCTCTTAATAGTTCCATGCAATTGTTTGCCATTTCTCTGACTTGCATATCCCACACCTTACCCTTCTCTCCATTACTTTAAGGCTGCGACTGCCAGTAAAATTTAGAATATGCCGATTGGGTTTTTTATGCAGGCTGCATTTCCGTTTAAGCGGCTTGGGATAGGTGCCTTTAAAATTGCATTGGAGAGCATATTTAATAGGTAAGGTTGCTATCTTGTGTCAAGATTTTCTCCATTTGTTGCTTAAAACTTAGGAACCAAGGTGTCAACATTTTGGCCCATGGATTGACCCACAAGCTGTTCTAGAATTATAAGTATTACTTTTGCTGGTTTTTCAACAGCATCCACACAAGCCGCTAGTGAGAAGCTAATCCTTTGTGGTAGAATTATTACTATTGCAAGCATTCATTGAAACCGTTTAATCTTGATTGACAGGATTCACCATACACCTCTCGAATATGTATCTTACGGGAAACCAAATCCTCTTGTTTTTAAGAGTGTTGAAACTGTATTGAGACAAATTCTCTCGTCTCACTGTGAGGATCATTTCGTGAACAAAGGAGACGTTGAATTTAATGGTTTCAAAACACTTTATATGATTGGTGACAATCCTGTGGTAGACATCAAAGGCGCACGAGAGGTTTGTCTTTTTTTAATTATTATAATTCCATCATCATGTTGACATTGAGTAAACATGTTGTCATCGGAAACCCAGCGTTCCTATTTGATATCGACTTGTGATTTCAGACAGGACGACCTTGGTTTTCTATTTTAACAAGGACAGGAGTTTTCAGGGGTGAAGAAAATCATGCTGAGTTTCCAGCCGATCTTGTAAGTTGATTGGTATAGATGAGTTTAGAAGTAATAGAAATTAAGGGCAATAATGTTCGACTATCACTTGTGTTTGAGTTCCTATTTCAATCCTTGCAGGTCGTTAATGATGTTGAAGAAGCAGTGGACTATATTTTGGAATTAGAAGGCATTTCTTGA

mRNA sequence

ATGTCTGAGTATGGTTTCAAAAAAGTTTTCTCCATTGATGAGTATGCTTCCTTCTTTGAGAATATTGATCCTGTATCCCAATACAAGAACTGGACAACCAAGCAAACTTTTGATTTGACTTTCAACCCTGAGAAGTTGATGAGAAGGCAGAGTGTTTTATCTGAAAGAGTGAAGGCAGCTTTTGTTGTCAGTGACCCTGTTGATTGGGGCAGGGATATCCAAGTTCTTTGTGATGTTTTAAGATCTGGAGGCCTTCCAGGACAGGAGGAGAATGGGGATCAAGTGCCTCTATATTTTGCTGCAGATGATTTTGAATATCAGGCTGCATTTCCGTTTAAGCGGCTTGGGATAGGTGCCTTTAAAATTGCATTGGAGAGCATATTTAATAGGATTCACCATACACCTCTCGAATATGTATCTTACGGGAAACCAAATCCTCTTGTTTTTAAGAGTGTTGAAACTGTATTGAGACAAATTCTCTCGTCTCACTGTGAGGATCATTTCGTGAACAAAGGAGACGTTGAATTTAATGGTTTCAAAACACTTTATATGATTGGTGACAATCCTGTGGTAGACATCAAAGGCGCACGAGAGACAGGACGACCTTGGTTTTCTATTTTAACAAGGACAGGAGTTTTCAGGGGTGAAGAAAATCATGCTGAGTTTCCAGCCGATCTTGTCGTTAATGATGTTGAAGAAGCAGTGGACTATATTTTGGAATTAGAAGGCATTTCTTGA

Coding sequence (CDS)

ATGTCTGAGTATGGTTTCAAAAAAGTTTTCTCCATTGATGAGTATGCTTCCTTCTTTGAGAATATTGATCCTGTATCCCAATACAAGAACTGGACAACCAAGCAAACTTTTGATTTGACTTTCAACCCTGAGAAGTTGATGAGAAGGCAGAGTGTTTTATCTGAAAGAGTGAAGGCAGCTTTTGTTGTCAGTGACCCTGTTGATTGGGGCAGGGATATCCAAGTTCTTTGTGATGTTTTAAGATCTGGAGGCCTTCCAGGACAGGAGGAGAATGGGGATCAAGTGCCTCTATATTTTGCTGCAGATGATTTTGAATATCAGGCTGCATTTCCGTTTAAGCGGCTTGGGATAGGTGCCTTTAAAATTGCATTGGAGAGCATATTTAATAGGATTCACCATACACCTCTCGAATATGTATCTTACGGGAAACCAAATCCTCTTGTTTTTAAGAGTGTTGAAACTGTATTGAGACAAATTCTCTCGTCTCACTGTGAGGATCATTTCGTGAACAAAGGAGACGTTGAATTTAATGGTTTCAAAACACTTTATATGATTGGTGACAATCCTGTGGTAGACATCAAAGGCGCACGAGAGACAGGACGACCTTGGTTTTCTATTTTAACAAGGACAGGAGTTTTCAGGGGTGAAGAAAATCATGCTGAGTTTCCAGCCGATCTTGTCGTTAATGATGTTGAAGAAGCAGTGGACTATATTTTGGAATTAGAAGGCATTTCTTGA

Protein sequence

MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAAFVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAFKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFKTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILELEGIS
Homology
BLAST of Lcy09g006000 vs. ExPASy Swiss-Prot
Match: O13899 (Uncharacterized CDP-alcohol phosphatidyltransferase class-I family protein C22A12.08c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22A12.08c PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.7e-20
Identity = 71/241 (29.46%), Postives = 113/241 (46.89%), Query Frame = 0

Query: 3   EYGFKKVFS-IDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAAF 62
           +YGFK V + +D  A       P + +     K   D              ++  ++A F
Sbjct: 137 KYGFKSVINELDVIAKLGTPFWPFTSFNEEDIKDAKDFD------------VTRPIEAVF 196

Query: 63  VVSDPVDWGRDIQVLCDV--LRSGGLPGQEENGDQVP-LYFAADDFEYQAAFPFKRLGIG 122
              DPV  G D+Q++ ++   ++G L    +  ++ P +YF+  D  +   +P  RLG G
Sbjct: 197 TYVDPVRLGLDLQLVMELGQSKNGVLGTVSKTANEGPDIYFSNADLIWPNEYPLPRLGQG 256

Query: 123 AFKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNG 182
           AF I  ES+F  +    L    YGKP+ L +   + +L   +  H      N        
Sbjct: 257 AFAICCESVFKELTGKDLRNTKYGKPHKLTYDYAKNIL---MKKHKTLGITNPP------ 316

Query: 183 FKTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYI 240
            K ++M+GDNP  DI+GA   G  W SIL RTG+F+G +N  ++ A  V ++V E V + 
Sbjct: 317 -KEIFMVGDNPESDIRGANNYG--WTSILVRTGIFQG-DNSPKYSAKHVSDNVWEGVRWA 352

BLAST of Lcy09g006000 vs. ExPASy Swiss-Prot
Match: P36151 (Uncharacterized protein YKR070W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKR070W PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.0e-20
Identity = 67/250 (26.80%), Postives = 114/250 (45.60%), Query Frame = 0

Query: 4   YGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAAFVV 63
           YGF+ V    +   +  +I P   +   + +Q  + + +   L       +++  A  V 
Sbjct: 111 YGFQDVVHQTDIVRYNRDIAP---FSGLSDEQVMEYSRDIPDL------TTKKFDAVLVF 170

Query: 64  SDPVDWGRDIQVLCDVLRS-----GGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIG 123
           +DP DW  DIQ++ D + S       L  ++     +P+YF+  D  +   +   R G G
Sbjct: 171 NDPHDWAADIQIISDAINSENGMLNTLRNEKSGKPSIPIYFSNQDLLWANPYKLNRFGQG 230

Query: 124 AFKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVL---RQILSSHCEDHFVNKGDV- 183
           AF++ +  ++  ++  PL+  + GKP  L +     VL    + LS         K  + 
Sbjct: 231 AFRLLVRRLYLELNGEPLQDYTLGKPTKLTYDFAHHVLIDWEKRLSGKIGQSVKQKLPLL 290

Query: 184 ----EFNGFKTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVND 241
                 + F  ++M+GDNP  DI GA+  G  W S L +TGV+   ++  E    L+VND
Sbjct: 291 GTKPSTSPFHAVFMVGDNPASDIIGAQNYG--WNSCLVKTGVYNEGDDLKECKPTLIVND 349

BLAST of Lcy09g006000 vs. ExPASy Swiss-Prot
Match: Q91WM2 (Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Mus musculus OX=10090 GN=Hdhd5 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 5.0e-14
Identity = 79/296 (26.69%), Postives = 120/296 (40.54%), Query Frame = 0

Query: 5   GFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAAFVVS 64
           GF+ V +IDE    F  +D V            DL   P K MR +S     ++   ++ 
Sbjct: 150 GFQNVVTIDELRLAFPELDMV------------DLQRRP-KTMRLRSDF-PAIEGVLLLG 209

Query: 65  DPVDWGRDIQVLCDVLRSGGLPG---QEENGDQVPLYFAADDFEYQAAFPFKRLGIGAFK 124
           +PV W  ++Q++ DVL S G PG          +P+  +  D  + A     R G G F 
Sbjct: 210 EPVRWETNLQLIMDVLLSNGHPGTGLATAPYPHLPVLASNMDLLWMAEAKMPRFGHGTFL 269

Query: 125 IALESIFNRIHHTPLEYVS-YGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGF- 184
           + LE+I+ +I    L+Y    GKP+ L ++  E V+RQ                E  G+ 
Sbjct: 270 LCLETIYRKITGNELKYEGLMGKPSILTYQYAEDVIRQ--------------QAERRGWA 329

Query: 185 ---KTLYMIGDNPVVDIKGA----------------RETG-----RP-----WFSILTRT 244
              + LY IGDNP+ D+ GA                ++TG     RP       SIL  T
Sbjct: 330 APIRKLYAIGDNPMSDVYGANLFHQYLQMANRGEEEQQTGGQQKQRPSATQSCASILVCT 389

BLAST of Lcy09g006000 vs. ExPASy Swiss-Prot
Match: Q9BXW7 (Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Homo sapiens OX=9606 GN=HDHD5 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.8e-11
Identity = 51/200 (25.50%), Postives = 84/200 (42.00%), Query Frame = 0

Query: 5   GFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAAFVVS 64
           GF+ V ++DE    F  +D V   +   T       F              R++   ++ 
Sbjct: 150 GFRNVVTVDELRMAFPLLDMVDLERRLKTTPLPRNDF-------------PRIEGVLLLG 209

Query: 65  DPVDWGRDIQVLCDVLRSGGLPG---QEENGDQVPLYFAADDFEYQAAFPFKRLGIGAFK 124
           +PV W   +Q++ DVL S G PG          +P+  +  D  + A     R G G F 
Sbjct: 210 EPVRWETSLQLIMDVLLSNGSPGAGLATPPYPHLPVLASNMDLLWMAEAKMPRFGHGTFL 269

Query: 125 IALESIFNRIHHTPLEYVS-YGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGF- 184
           + LE+I+ ++    L Y    GKP+ L ++  E ++R+                E  G+ 
Sbjct: 270 LCLETIYQKVTGKELRYEGLMGKPSILTYQYAEDLIRR--------------QAERRGWA 322

Query: 185 ---KTLYMIGDNPVVDIKGA 197
              + LY +GDNP+ D+ GA
Sbjct: 330 APIRKLYAVGDNPMSDVYGA 322

BLAST of Lcy09g006000 vs. ExPASy Swiss-Prot
Match: Q0VD18 (Phospholysine phosphohistidine inorganic pyrophosphate phosphatase OS=Bos taurus OX=9913 GN=LHPP PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.1e-04
Identity = 43/146 (29.45%), Postives = 63/146 (43.15%), Query Frame = 0

Query: 96  PLYFAADDFEYQAAFPFKRLGIGAFKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETV 155
           P+ F+     Y        L +G +  ALE      +   +E    GKP+P  FKS    
Sbjct: 148 PVLFSLGKGRYYKETSGLMLDVGPYMKALE------YACGIEAEVVGKPSPEFFKSA--- 207

Query: 156 LRQILSSHCEDHFVNKGDVEFNGFKTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFR- 215
              +     E H                MIGD+ V D+ GA+  G    ++  RTG FR 
Sbjct: 208 ---LQEMGVEAH-------------EAIMIGDDIVGDVGGAQRYGMR--ALQVRTGKFRP 266

Query: 216 GEENHAEFPADLVVNDVEEAVDYILE 241
            +E+H E  AD  V+++ EAVD +L+
Sbjct: 268 SDEHHPEVKADGYVDNLAEAVDLLLQ 266

BLAST of Lcy09g006000 vs. ExPASy TrEMBL
Match: A0A6J1JXI7 (uncharacterized protein YKR070W-like OS=Cucurbita maxima OX=3661 GN=LOC111489204 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.2e-127
Identity = 224/245 (91.43%), Postives = 234/245 (95.51%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FDLT NPEKLMR+QSVLSERVKAA
Sbjct: 166 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDLTCNPEKLMRKQSVLSERVKAA 225

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYFAADDFEYQAAFPFKRLGIGAF
Sbjct: 226 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFAADDFEYQAAFPFKRLGIGAF 285

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DH VNK D+EFNGF+
Sbjct: 286 KIALESIFNRIHHRPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHLVNKEDIEFNGFR 345

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           TLYMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEENH EFPADLVVN VEEAV+YILE
Sbjct: 346 TLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILE 405

Query: 241 LEGIS 246
            E IS
Sbjct: 406 SECIS 409

BLAST of Lcy09g006000 vs. ExPASy TrEMBL
Match: A0A6J1FF73 (uncharacterized protein YKR070W-like OS=Cucurbita moschata OX=3662 GN=LOC111444927 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.3e-126
Identity = 223/245 (91.02%), Postives = 232/245 (94.69%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FD T NPEKLMR+QSVLSERVKAA
Sbjct: 166 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDSTCNPEKLMRKQSVLSERVKAA 225

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYFAADDFEYQAAFP KRLGIGAF
Sbjct: 226 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFAADDFEYQAAFPLKRLGIGAF 285

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DHFVNK DVEFNGF+
Sbjct: 286 KIALESIFNRIHHRPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFR 345

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           T YMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEENH EFPADLVVN VEEAV+YILE
Sbjct: 346 TFYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILE 405

Query: 241 LEGIS 246
            E IS
Sbjct: 406 SECIS 409

BLAST of Lcy09g006000 vs. ExPASy TrEMBL
Match: A0A5D3C5R2 (Hydrolase family protein / HAD-superfamily protein isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G002260 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.6e-117
Identity = 209/246 (84.96%), Postives = 218/246 (88.62%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFSI EYASFFENIDPVSQYK+WT+KQ FD   +P   MRRQSVLSERVKAA
Sbjct: 304 MSEYGFKKVFSIGEYASFFENIDPVSQYKSWTSKQAFDSNCDPHGSMRRQSVLSERVKAA 363

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPG-QEENGDQVPLYFAADDFEYQAAFPFKRLGIGA 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPG Q  N +QVPLYFAADD EYQ AFPFKRLGIGA
Sbjct: 364 FVVSDPVDWGRDIQVLCDVLRSGGLPGHQNGNLNQVPLYFAADDLEYQGAFPFKRLGIGA 423

Query: 121 FKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGF 180
           FKIALESIF RIHH PLEYV YGKPNPLVFK+VE V RQIL SHC+DHFVNKGD+E NGF
Sbjct: 424 FKIALESIFERIHHIPLEYVCYGKPNPLVFKNVEAVFRQILLSHCDDHFVNKGDIEVNGF 483

Query: 181 KTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYIL 240
           KTLYMIGDNPVVDIKGARE GRPWFSILTRTGVF+G+ENH EFPADLVVN VEEAVDYI 
Sbjct: 484 KTLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFKGKENHVEFPADLVVNTVEEAVDYIF 543

Query: 241 ELEGIS 246
             E IS
Sbjct: 544 ASEHIS 549

BLAST of Lcy09g006000 vs. ExPASy TrEMBL
Match: A0A1S3CK22 (uncharacterized protein YKR070W-like OS=Cucumis melo OX=3656 GN=LOC103501676 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.6e-117
Identity = 209/246 (84.96%), Postives = 218/246 (88.62%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFSI EYASFFENIDPVSQYK+WT+KQ FD   +P   MRRQSVLSERVKAA
Sbjct: 134 MSEYGFKKVFSIGEYASFFENIDPVSQYKSWTSKQAFDSNCDPHGSMRRQSVLSERVKAA 193

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPG-QEENGDQVPLYFAADDFEYQAAFPFKRLGIGA 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPG Q  N +QVPLYFAADD EYQ AFPFKRLGIGA
Sbjct: 194 FVVSDPVDWGRDIQVLCDVLRSGGLPGHQNGNLNQVPLYFAADDLEYQGAFPFKRLGIGA 253

Query: 121 FKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGF 180
           FKIALESIF RIHH PLEYV YGKPNPLVFK+VE V RQIL SHC+DHFVNKGD+E NGF
Sbjct: 254 FKIALESIFERIHHIPLEYVCYGKPNPLVFKNVEAVFRQILLSHCDDHFVNKGDIEVNGF 313

Query: 181 KTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYIL 240
           KTLYMIGDNPVVDIKGARE GRPWFSILTRTGVF+G+ENH EFPADLVVN VEEAVDYI 
Sbjct: 314 KTLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFKGKENHVEFPADLVVNTVEEAVDYIF 373

Query: 241 ELEGIS 246
             E IS
Sbjct: 374 ASEHIS 379

BLAST of Lcy09g006000 vs. ExPASy TrEMBL
Match: A0A0A0KU31 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G497050 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.8e-115
Identity = 206/246 (83.74%), Postives = 217/246 (88.21%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFSI EYASFFENIDPVS YK+WT+KQ F+   NP +LMRRQSVLSERVKAA
Sbjct: 136 MSEYGFKKVFSIGEYASFFENIDPVSHYKSWTSKQAFNSNCNPHELMRRQSVLSERVKAA 195

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPG-QEENGDQVPLYFAADDFEYQAAFPFKRLGIGA 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPG Q  N +QVPLYFAADD EYQ AFP KRLGIGA
Sbjct: 196 FVVSDPVDWGRDIQVLCDVLRSGGLPGYQNGNLNQVPLYFAADDLEYQGAFPLKRLGIGA 255

Query: 121 FKIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGF 180
           FKIALESIFNRIHH PLEYV YGKPNPLVFK+VE V +QIL SHC+DHFVNKGD+E NGF
Sbjct: 256 FKIALESIFNRIHHIPLEYVCYGKPNPLVFKNVEAVFKQILLSHCDDHFVNKGDIEVNGF 315

Query: 181 KTLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYIL 240
           K LYMIGDNPVVDIKGARE GRPWFSILTRTGVF+ +ENH EFPADLVVN VEEAVDYI 
Sbjct: 316 KKLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFKDKENHIEFPADLVVNTVEEAVDYIF 375

Query: 241 ELEGIS 246
             E IS
Sbjct: 376 ASEHIS 381

BLAST of Lcy09g006000 vs. NCBI nr
Match: XP_022993080.1 (uncharacterized protein YKR070W-like [Cucurbita maxima])

HSP 1 Score: 465.7 bits (1197), Expect = 2.5e-127
Identity = 224/245 (91.43%), Postives = 234/245 (95.51%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FDLT NPEKLMR+QSVLSERVKAA
Sbjct: 166 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDLTCNPEKLMRKQSVLSERVKAA 225

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYFAADDFEYQAAFPFKRLGIGAF
Sbjct: 226 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFAADDFEYQAAFPFKRLGIGAF 285

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DH VNK D+EFNGF+
Sbjct: 286 KIALESIFNRIHHRPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHLVNKEDIEFNGFR 345

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           TLYMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEENH EFPADLVVN VEEAV+YILE
Sbjct: 346 TLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILE 405

Query: 241 LEGIS 246
            E IS
Sbjct: 406 SECIS 409

BLAST of Lcy09g006000 vs. NCBI nr
Match: XP_023549962.1 (uncharacterized protein YKR070W-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549963.1 uncharacterized protein YKR070W-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 463.0 bits (1190), Expect = 1.6e-126
Identity = 224/245 (91.43%), Postives = 233/245 (95.10%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FDLT NPEKLMR+QSVLSERVKAA
Sbjct: 143 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDLTCNPEKLMRKQSVLSERVKAA 202

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYFAADDFEYQAAFPFKRLGIGAF
Sbjct: 203 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFAADDFEYQAAFPFKRLGIGAF 262

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DHFVNK D+EFN FK
Sbjct: 263 KIALESIFNRIHHRPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHFVNKEDIEFNSFK 322

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           TLYMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEEN  EFPADLVVN VEEAV+YILE
Sbjct: 323 TLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENQDEFPADLVVNTVEEAVEYILE 382

Query: 241 LEGIS 246
            E IS
Sbjct: 383 SECIS 386

BLAST of Lcy09g006000 vs. NCBI nr
Match: KAG6578738.1 (hypothetical protein SDJN03_23186, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 462.6 bits (1189), Expect = 2.1e-126
Identity = 223/245 (91.02%), Postives = 233/245 (95.10%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FD T NPEKLMR+QSVLSERVKAA
Sbjct: 166 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDSTCNPEKLMRKQSVLSERVKAA 225

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYF+ADDFEYQAAFP KRLGIGAF
Sbjct: 226 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFSADDFEYQAAFPLKRLGIGAF 285

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DHFVNK DVEFNGF+
Sbjct: 286 KIALESIFNRIHHKPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFR 345

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           TLYMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEENH EFPADLVVN VEEAV+YILE
Sbjct: 346 TLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILE 405

Query: 241 LEGIS 246
            E IS
Sbjct: 406 SECIS 409

BLAST of Lcy09g006000 vs. NCBI nr
Match: XP_022938837.1 (uncharacterized protein YKR070W-like [Cucurbita moschata])

HSP 1 Score: 462.2 bits (1188), Expect = 2.7e-126
Identity = 223/245 (91.02%), Postives = 232/245 (94.69%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FD T NPEKLMR+QSVLSERVKAA
Sbjct: 166 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDSTCNPEKLMRKQSVLSERVKAA 225

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYFAADDFEYQAAFP KRLGIGAF
Sbjct: 226 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFAADDFEYQAAFPLKRLGIGAF 285

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DHFVNK DVEFNGF+
Sbjct: 286 KIALESIFNRIHHRPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFR 345

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           T YMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEENH EFPADLVVN VEEAV+YILE
Sbjct: 346 TFYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILE 405

Query: 241 LEGIS 246
            E IS
Sbjct: 406 SECIS 409

BLAST of Lcy09g006000 vs. NCBI nr
Match: KAG7016268.1 (hypothetical protein SDJN02_21374 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.1 bits (1185), Expect = 6.1e-126
Identity = 222/245 (90.61%), Postives = 232/245 (94.69%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MSEYGFKKVFS+DEYASFFENIDPVSQYKNWT++Q FD T NPEKLMR+Q VLSERVKAA
Sbjct: 166 MSEYGFKKVFSLDEYASFFENIDPVSQYKNWTSQQAFDSTCNPEKLMRKQGVLSERVKAA 225

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           FVVSDPVDWGRDIQVLCDVLRSGGLPGQ ENG+QVPLYF+ADDFEYQAAFP KRLGIGAF
Sbjct: 226 FVVSDPVDWGRDIQVLCDVLRSGGLPGQ-ENGNQVPLYFSADDFEYQAAFPLKRLGIGAF 285

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           KIALESIFNRIHH PLEYVSYGKPNPLVFK+VETVLRQILSSHC+DHFVNK DVEFNGF+
Sbjct: 286 KIALESIFNRIHHRPLEYVSYGKPNPLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFR 345

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           TLYMIGDNPVVDIKGARE GRPWFSILTRTGVFRGEENH EFPADLVVN VEEAV+YILE
Sbjct: 346 TLYMIGDNPVVDIKGAREAGRPWFSILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILE 405

Query: 241 LEGIS 246
            E IS
Sbjct: 406 SECIS 409

BLAST of Lcy09g006000 vs. TAIR 10
Match: AT3G45740.1 (hydrolase family protein / HAD-superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 4.0e-83
Identity = 151/242 (62.40%), Postives = 187/242 (77.27%), Query Frame = 0

Query: 1   MSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQTFDLTFNPEKLMRRQSVLSERVKAA 60
           MS YGFK V S+DEYAS+F+NIDP++ YK    +Q        ++L  R+ VLS+RV+AA
Sbjct: 139 MSNYGFKNVISMDEYASYFDNIDPLAPYKKLMFRQD-----GHKELRSREDVLSQRVQAA 198

Query: 61  FVVSDPVDWGRDIQVLCDVLRSGGLPGQEENGDQVPLYFAADDFEYQAAFPFKRLGIGAF 120
           F+VSDPVDW RDIQVLCD+LR+GGLPG +E G Q  LY A DD +YQ  FP +RLG+GAF
Sbjct: 199 FIVSDPVDWSRDIQVLCDILRTGGLPG-KEIGPQPHLYIANDDLDYQTEFPTERLGMGAF 258

Query: 121 KIALESIFNRIHHTPLEYVSYGKPNPLVFKSVETVLRQILSSHCEDHFVNKGDVEFNGFK 180
           +IALESIFNRIH  PLEY S+GKPNP VFK+ E VL++I +S    +  N+G    + FK
Sbjct: 259 RIALESIFNRIHEKPLEYTSFGKPNPFVFKNAEDVLKEIATS---PYSSNQGS---HHFK 318

Query: 181 TLYMIGDNPVVDIKGARETGRPWFSILTRTGVFRGEENHAEFPADLVVNDVEEAVDYILE 240
           TLYMIGDNP +DI+GAR+ G PWFSILTRTGVF+G++NH+EFPADLVV+ VEEAVD+IL 
Sbjct: 319 TLYMIGDNPKIDIRGARQAGTPWFSILTRTGVFKGKDNHSEFPADLVVDTVEEAVDFILT 368

Query: 241 LE 243
            E
Sbjct: 379 RE 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O138994.7e-2029.46Uncharacterized CDP-alcohol phosphatidyltransferase class-I family protein C22A1... [more]
P361518.0e-2026.80Uncharacterized protein YKR070W OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Q91WM25.0e-1426.69Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Mus musculus OX=1009... [more]
Q9BXW78.8e-1125.50Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Homo sapiens OX=9606... [more]
Q0VD182.1e-0429.45Phospholysine phosphohistidine inorganic pyrophosphate phosphatase OS=Bos taurus... [more]
Match NameE-valueIdentityDescription
A0A6J1JXI71.2e-12791.43uncharacterized protein YKR070W-like OS=Cucurbita maxima OX=3661 GN=LOC111489204... [more]
A0A6J1FF731.3e-12691.02uncharacterized protein YKR070W-like OS=Cucurbita moschata OX=3662 GN=LOC1114449... [more]
A0A5D3C5R25.6e-11784.96Hydrolase family protein / HAD-superfamily protein isoform 2 OS=Cucumis melo var... [more]
A0A1S3CK225.6e-11784.96uncharacterized protein YKR070W-like OS=Cucumis melo OX=3656 GN=LOC103501676 PE=... [more]
A0A0A0KU311.8e-11583.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G497050 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022993080.12.5e-12791.43uncharacterized protein YKR070W-like [Cucurbita maxima][more]
XP_023549962.11.6e-12691.43uncharacterized protein YKR070W-like isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
KAG6578738.12.1e-12691.02hypothetical protein SDJN03_23186, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022938837.12.7e-12691.02uncharacterized protein YKR070W-like [Cucurbita moschata][more]
KAG7016268.16.1e-12690.61hypothetical protein SDJN02_21374 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G45740.14.0e-8362.40hydrolase family protein / HAD-superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 1..141
e-value: 7.4E-59
score: 201.3
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 142..235
e-value: 7.4E-59
score: 201.3
IPR006353HAD-superfamily hydrolase, subfamily IIA, CECR5TIGRFAMTIGR01456TIGR01456coord: 1..239
e-value: 2.5E-71
score: 238.1
NoneNo IPR availablePFAMPF13242Hydrolase_likecoord: 141..234
e-value: 1.5E-15
score: 57.0
NoneNo IPR availablePANTHERPTHR14269CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATEDcoord: 1..242
NoneNo IPR availablePANTHERPTHR14269:SF41HYDROLASE FAMILY PROTEIN / HAD-SUPERFAMILY PROTEINcoord: 1..242
IPR006357HAD-superfamily hydrolase, subfamily IIATIGRFAMTIGR01460TIGR01460coord: 1..211
e-value: 2.7E-30
score: 103.9
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 53..235

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g006000.1Lcy09g006000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001732 formation of cytoplasmic translation initiation complex
biological_process GO:0046474 glycerophospholipid biosynthetic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016282 eukaryotic 43S preinitiation complex
cellular_component GO:0033290 eukaryotic 48S preinitiation complex
cellular_component GO:0005852 eukaryotic translation initiation factor 3 complex
cellular_component GO:0005739 mitochondrion
molecular_function GO:0070122 isopeptidase activity
molecular_function GO:0008237 metallopeptidase activity
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0031369 translation initiation factor binding