Lcy09g004300 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g004300
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	metalloendoproteinase 1-like
Location	Chr09: 3912092 .. 3913039 (-)
RNA-Seq Expression	Lcy09g004300
Synteny	Lcy09g004300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCTCAACACATCAATCTCAATTTGTCTCCAAGTTTCTTGTCAACATGGATTTTAAATTATCTCTCCAACTCTTCCTACTTCTCGCCTTGGTCGCACCTCATGCGATCTCGGCACGTGATCTCCACATCCGCAAATCGTCTCGGTTTTCGTTGCCTCGACAACTTCTAGGAAGTCGCAAGGGGCACAACATAGAAGGAACGCACAACGTAAAAACTTATCTCCGACGTTACGGTTACTTAAGCAGCAATGTCGTCGAGGCCAACAACGACAATGCCTTCGACGACGACTTGGAATATGCCGTTAAAAGATACCAAAAATTCTTCAAGCTCAACGTGAGTGGCATCTTGGACGCGGATACATTACATCTAATGTCACAGCCTCGATGCTCAGTTCCCGACGTGTTCGACGACAAGATCGTGAACGCAACGGGGTCGCGCAGGGCCATAGGAACTCTTTACACATTTTTCCCAGGGGAACCCAAATGGTCCAGAGAGAAGCTAAAATACGCATTTGTTAACGATTTCCCATCGTACTTCGAGGAGCCAGTGAATCAAGCCCTTAAGGATTGGGCTTTGTACAGCCCATTTGAATTCACAGAAGTTGATCATGATGCTGTAGCAGATATAAGGATAAGCTTTGAACTGGGGGACCATGGAGATGGGCATCCGTTCATAAATGGATCGGGAGTGTTGGCGCATGCTTTTGCGCCAGAAAATGGGAGGCTGCACTTCAATTCGATGATGTCTTGGTCGAATGGAGCTGTGGATGAAACGTTTGACGTGCGCAGTGTAGCAATGCATGAGCTTGGACATAATCTTGGGCTCGGCCACAGCACCTCAAAACAAGCCATCATGTTCCCGACTATTCCTCCCGCTACCGTCAAGGAAATAAATTTCGACGATATTGATGGACTCAACGAGTTATATGGCAATTATTTAGGTTAA

mRNA sequence

ATGATCTCAACACATCAATCTCAATTTGTCTCCAAGTTTCTTGTCAACATGGATTTTAAATTATCTCTCCAACTCTTCCTACTTCTCGCCTTGGTCGCACCTCATGCGATCTCGGCACGTGATCTCCACATCCGCAAATCGTCTCGGTTTTCGTTGCCTCGACAACTTCTAGGAAGTCGCAAGGGGCACAACATAGAAGGAACGCACAACGTAAAAACTTATCTCCGACGTTACGGTTACTTAAGCAGCAATGTCGTCGAGGCCAACAACGACAATGCCTTCGACGACGACTTGGAATATGCCGTTAAAAGATACCAAAAATTCTTCAAGCTCAACGTGAGTGGCATCTTGGACGCGGATACATTACATCTAATGTCACAGCCTCGATGCTCAGTTCCCGACGTGTTCGACGACAAGATCGTGAACGCAACGGGGTCGCGCAGGGCCATAGGAACTCTTTACACATTTTTCCCAGGGGAACCCAAATGGTCCAGAGAGAAGCTAAAATACGCATTTGTTAACGATTTCCCATCGTACTTCGAGGAGCCAGTGAATCAAGCCCTTAAGGATTGGGCTTTGTACAGCCCATTTGAATTCACAGAAGTTGATCATGATGCTGTAGCAGATATAAGGATAAGCTTTGAACTGGGGGACCATGGAGATGGGCATCCGTTCATAAATGGATCGGGAGTGTTGGCGCATGCTTTTGCGCCAGAAAATGGGAGGCTGCACTTCAATTCGATGATGTCTTGGTCGAATGGAGCTGTGGATGAAACGTTTGACGTGCGCAGTGTAGCAATGCATGAGCTTGGACATAATCTTGGGCTCGGCCACAGCACCTCAAAACAAGCCATCATGTTCCCGACTATTCCTCCCGCTACCGTCAAGGAAATAAATTTCGACGATATTGATGGACTCAACGAGTTATATGGCAATTATTTAGGTTAA

Coding sequence (CDS)

ATGATCTCAACACATCAATCTCAATTTGTCTCCAAGTTTCTTGTCAACATGGATTTTAAATTATCTCTCCAACTCTTCCTACTTCTCGCCTTGGTCGCACCTCATGCGATCTCGGCACGTGATCTCCACATCCGCAAATCGTCTCGGTTTTCGTTGCCTCGACAACTTCTAGGAAGTCGCAAGGGGCACAACATAGAAGGAACGCACAACGTAAAAACTTATCTCCGACGTTACGGTTACTTAAGCAGCAATGTCGTCGAGGCCAACAACGACAATGCCTTCGACGACGACTTGGAATATGCCGTTAAAAGATACCAAAAATTCTTCAAGCTCAACGTGAGTGGCATCTTGGACGCGGATACATTACATCTAATGTCACAGCCTCGATGCTCAGTTCCCGACGTGTTCGACGACAAGATCGTGAACGCAACGGGGTCGCGCAGGGCCATAGGAACTCTTTACACATTTTTCCCAGGGGAACCCAAATGGTCCAGAGAGAAGCTAAAATACGCATTTGTTAACGATTTCCCATCGTACTTCGAGGAGCCAGTGAATCAAGCCCTTAAGGATTGGGCTTTGTACAGCCCATTTGAATTCACAGAAGTTGATCATGATGCTGTAGCAGATATAAGGATAAGCTTTGAACTGGGGGACCATGGAGATGGGCATCCGTTCATAAATGGATCGGGAGTGTTGGCGCATGCTTTTGCGCCAGAAAATGGGAGGCTGCACTTCAATTCGATGATGTCTTGGTCGAATGGAGCTGTGGATGAAACGTTTGACGTGCGCAGTGTAGCAATGCATGAGCTTGGACATAATCTTGGGCTCGGCCACAGCACCTCAAAACAAGCCATCATGTTCCCGACTATTCCTCCCGCTACCGTCAAGGAAATAAATTTCGACGATATTGATGGACTCAACGAGTTATATGGCAATTATTTAGGTTAA

Protein sequence

MISTHQSQFVSKFLVNMDFKLSLQLFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDVFDDKIVNATGSRRAIGTLYTFFPGEPKWSREKLKYAFVNDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFNSMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGLNELYGNYLG

Homology

[Close]

BLAST of Lcy09g004300 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.8e-47
Identity = 109/271 (40.22%), Postives = 145/271 (53.51%), Query Frame = 0


Query: 55  QLLGSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVS 114
           +L G   G NI G   +K Y RR+GY+++     N  + FDD L+ A+  YQK F L V+
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYITTT---GNCTDDFDDVLQSAINTYQKNFNLKVT 106

Query: 115 GILDADTLHLMSQPRCSVPDVFDDKIVNATGSRRAIGTLYTFFPGEPKWSREK--LKYAF 174
           G LD+ TL  + +PRC  PD+ D       G        Y+FFPG+P+W + K  L YAF
Sbjct: 107 GKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAF 166

Query: 175 V--NDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSG 234
              N+     +   ++A   WA  +P  FT  +    ADI I F  G+HGDG PF    G
Sbjct: 167 APQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG 226

Query: 235 VLAHAFAPENGRLHFNSMMSW--SNGAVDE-------TFDVRSVAMHELGHNLGLGHSTS 294
            LAHA +P  G LH +    W  SNG +           D+ SVA+HE+GH LGLGHS+ 
Sbjct: 227 TLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 286

Query: 295 KQAIMFPTIPPATVK-EINFDDIDGLNELYG 312
           + AIMFP I     K E+  DDI+G+  LYG
Sbjct: 287 EDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lcy09g004300 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.7e-46
Identity = 114/315 (36.19%), Postives = 166/315 (52.70%), Query Frame = 0


Query: 26  FLLLALVAPHAISARD--------------LHIRKSSRFSLPRQLLGSRKGHNIEGTHNV 85
           F+L+    P+   AR+              +H+  S+     R L+  + G ++ G   +
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSR-LVDVQIGSHVSGVSEL 74

Query: 86  KTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCS 145
           K YL R+GY++    E  +D  FD  LE A+  YQ+   L ++G LD  T+ LMS PRC 
Sbjct: 75  KRYLHRFGYVNDG-SEIFSD-VFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCG 134

Query: 146 VPD---VFDDKIVNATGSRRAIGTLYTFFPGEPKWSREKLKYAF--VNDFPSYFEEPV-- 205
           V D     ++  ++ T         YT+F G+PKW+R+ L YA    +       E V  
Sbjct: 135 VSDTHMTINNDFLHTTAH-------YTYFNGKPKWNRDTLTYAISKTHKLDYLTSEDVKT 194

Query: 206 --NQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGR 265
              +A   W+   P  F EVD    AD++I F  GDHGDG PF    G LAHAFAPENGR
Sbjct: 195 VFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGR 254

Query: 266 LHFNSMMSW-----SNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVK- 312
           LH ++  +W       G+ +   D+ SVA HE+GH LGLGHS+ + A+M+P++ P T K 
Sbjct: 255 LHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKV 314

[Close]

BLAST of Lcy09g004300 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 182.6 bits (462), Expect = 7.1e-45
Identity = 105/263 (39.92%), Postives = 144/263 (54.75%), Query Frame = 0


Query: 62  GHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADT 121
           G N +G  NVK Y    GY+ +     + D+ FDD L  A+K YQK + LNV+G  D +T
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPN---APHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINT 107

Query: 122 LHLMSQPRCSVPDVFDDKIVNATGSRRAIGTL--YTFFPGEPKW--SREKLKYAFVND-- 181
           L  +  PRC VPD+    I+N T    + G +  YTFF   P+W     +L YAF  +  
Sbjct: 108 LKQIMTPRCGVPDI----IIN-TNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPR 167

Query: 182 FPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHA 241
               F+  + +A   W       F E      A+I+I F   +HGD +PF    G+L HA
Sbjct: 168 LDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHA 227

Query: 242 FAPENGRLHFNSMMSW------SNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPT 301
           FAP +GR HF++   W      +   V   FD+ SVA+HE+GH LGLGHS+  +AIM+P+
Sbjct: 228 FAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPS 287

Query: 302 IPPATVK-EINFDDIDGLNELYG 312
           IPP T K  +  DDIDG+ +LYG
Sbjct: 288 IPPRTRKVNLAQDDIDGIRKLYG 302

[Close]

BLAST of Lcy09g004300 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.6e-44
Identity = 103/255 (40.39%), Postives = 143/255 (56.08%), Query Frame = 0


Query: 71  VKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRC 130
           +K +L++YGYL  N  + ++D +F    E A+ RYQK   L ++G  D+DTL  +  PRC
Sbjct: 53  IKRHLQQYGYLPQN--KESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQILLPRC 112

Query: 131 SVPDVFDDKIVNATGSRRAIGTLYTFFPGEPKWSRE---KLKYAFVND------FPSYFE 190
             PD  + K      +    G  Y +FPG P+W+R+   KL YAF  +       P+   
Sbjct: 113 GFPDDVEPKT-----APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIR 172

Query: 191 EPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENG 250
               +A   WA   P  F E +   +ADI+I F  GDHGDG PF    GVLAH F+PENG
Sbjct: 173 RVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENG 232

Query: 251 RLHFNSMMSWSNGAVDE----TFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVK- 310
           RLH +   +W+    +E      D+ SVA+HE+GH LGLGHS+ K A M+PT+ P + K 
Sbjct: 233 RLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKV 292

Query: 311 EINFDDIDGLNELYG 312
            +N DD+ G+  LYG
Sbjct: 293 NLNMDDVVGVQSLYG 296

[Close]

BLAST of Lcy09g004300 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.6e-44
Identity = 103/277 (37.18%), Postives = 149/277 (53.79%), Query Frame = 0


Query: 58  GSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGIL 117
           G   G N++G + +K Y +R+GY+       N  + FDD L+ AV+ YQ  F LNV+G L
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYI-PETFSGNFTDDFDDILKAAVELYQTNFNLNVTGEL 108

Query: 118 DADTLHLMSQPRCSVPDVFDDKIVNATGSRRAIGT-----------LYTFFPGEPKWSRE 177
           DA T+  +  PRC  PDV +   +   G R+                YT FPGEP+W R 
Sbjct: 109 DALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRN 168

Query: 178 K--LKYAF--VNDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDG 237
           +  L YAF   N      +   ++A   W+  +   FT  +  + +DI I F  GDHGDG
Sbjct: 169 RRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDG 228

Query: 238 HPFINGSGVLAHAFAPENGRLHFNSMMSW-------SNGAVDETFDVRSVAMHELGHNLG 297
            PF    G LAHAF+P +G+ H ++  +W       S  +V    D+ SVA+HE+GH LG
Sbjct: 229 EPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLG 288

Query: 298 LGHSTSKQAIMFPTIPPATVK-EINFDDIDGLNELYG 312
           LGHS+ +++IM+PTI     K ++  DD++G+  LYG
Sbjct: 289 LGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Lcy09g004300 vs. ExPASy TrEMBL
Match: A0A6J1H7I1 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.6e-84
Identity = 167/304 (54.93%), Postives = 212/304 (69.74%), Query Frame = 0


Query: 17  MDFKLSLQ-LFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD +  LQ +F+L+A +APHAI AR LHI K S    P+ L+GS KGHNIEG HNV+ +L
Sbjct: 1   MDLRSCLQVMFILVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      N FDD+LE+A+K YQKFF LNVSGILDA+TL LMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--ETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 137 FDDKIVNATGS------RRAIGTLYTFFPG-EPKWSREK--LKYAFVNDFPSYFEEPVNQ 196
           F    +N TG          IG  Y+FF   +PKWS  K  LKY F+  FP  ++  V Q
Sbjct: 121 F----INETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQ 180

Query: 197 ALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFN 256
           A+  W+  S F F+E   D   D+RISFE+GDHGDG PF + +G LAHAFAPE+GRLHFN
Sbjct: 181 AMISWSFNSVFAFSEGSTDN-TDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFN 240

Query: 257 SMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGL 311
           +  +W++GA  + FDV SVA+HELGH LGLGHS  + A+M+P++PP   KE++ DD+ G+
Sbjct: 241 AQHAWADGAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGI 297

[Close]

BLAST of Lcy09g004300 vs. ExPASy TrEMBL
Match: A0A6J1H7Q4 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.7e-81
Identity = 162/301 (53.82%), Postives = 206/301 (68.44%), Query Frame = 0


Query: 17  MDFKLSLQL-FLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD K  LQL F+L+A +APHAI AR LH  K S    P+ L+GS KGHN+EG HNV+ +L
Sbjct: 1   MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAFL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      NAFDD+LE+A+K YQKFF LNVSGILD +TL LMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--DTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPDV 120

Query: 137 FDDKIVNATGSRRAIGTLYTFF-PGEPKW--SREKLKYAFVNDFPSYFEEPVNQALKDWA 196
           F                 YTFF   +PKW  S+  LKYAFV +FP  ++  V +A   W+
Sbjct: 121 FRID--------------YTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWS 180

Query: 197 LYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFNSMMSWS 256
             + F FTE   D   D++ISFE+GDHGDG+PF+ GSGVLAHAFAPE+GRLHFN+  +W+
Sbjct: 181 SVTGFTFTEGSVDN-TDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWA 240

Query: 257 NGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGLNELYGN 314
           +GA    +DV +VA+HELGH LGLGHS  + A+M+P  PP  V  ++ DD++G+  LY  
Sbjct: 241 DGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDDVNGIMALYNG 284

[Close]

BLAST of Lcy09g004300 vs. ExPASy TrEMBL
Match: A0A0A0KX75 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632100 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.8e-81
Identity = 167/310 (53.87%), Postives = 211/310 (68.06%), Query Frame = 0


Query: 17  MDFK-LSLQLFLLLA--LVAPHAISARDL-HIRKSSRFSLPRQLLGSRKGHNIEGTHNVK 76
           MDFK L LQLFLLL   L+  H IS+RDL HI   S+F  P+ L+GSRKGHN+EGTH +K
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 77  TYLRRYGYLSS--NVVEANN--DNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQP 136
           TYL+RYGYLS   N+++ N   +NA+D+ LE ++K+YQKFF+LN SGILD +TL  MSQ 
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 137 RCSVPDVF--DDKIVNATGSRRAIGTLYTFFPGEPKWSREK---LKYAFVNDFPSYFEEP 196
           RCSVPD F  DD   + T S   +G+ + FFPG PKW   K   L ++F+N+FP  F+  
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 197 VNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRL 256
           V  A   W   S F FTEV     +DI+ISFE+GDHGDGHPF    GVLAHAF P +GRL
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTPTDGRL 240

Query: 257 HFNSMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDI 314
           HFN    +S+   +  + VRSVA+HELGH+LGL H+    AIM+PT+PP   K IN DD+
Sbjct: 241 HFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDV 300

[Close]

BLAST of Lcy09g004300 vs. ExPASy TrEMBL
Match: A0A6J1BVR3 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 4.1e-80
Identity = 164/308 (53.25%), Postives = 207/308 (67.21%), Query Frame = 0


Query: 18  DFKLSLQL----FLLLALVAPHAISARDLH---IRKSSRFSLPRQLLGSRKGHNIEGTHN 77
           +FK SL L    F+LLA +A H  SAR LH     KSS     + LLGSRKGHNI+G H+
Sbjct: 3   EFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHH 62

Query: 78  VKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRC 137
           VKTYL+RYGY +         NAFDD LE A+K YQKFF LNVSGILD +TL LMSQPRC
Sbjct: 63  VKTYLKRYGYFTH-----TQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRC 122

Query: 138 SVPDVFDDKIVNATG----SRRAIGTLYTFFPGEPKW--SREKLKYAFVNDFPSYFEEPV 197
           +VPD+F ++     G        +G+ Y FFP  PKW  S+ +L+Y++ +DFP  F++PV
Sbjct: 123 AVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPV 182

Query: 198 NQALKDWALYSPFEFTEV-DHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRL 257
           N AL+ W+ Y  F F EV + D  AD++ISFE+G+HGDGHPFI  SGVLAHAFAP +GR 
Sbjct: 183 NNALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRF 242

Query: 258 HFNSMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDI 312
           HFN    W+ GA     DV SVA+HE+GH LGLGHS +++AIM+P I P  VK +  D++
Sbjct: 243 HFNVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNV 302

[Close]

BLAST of Lcy09g004300 vs. ExPASy TrEMBL
Match: A0A6J1H5Y2 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.7e-79
Identity = 155/284 (54.58%), Postives = 198/284 (69.72%), Query Frame = 0


Query: 39  ARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDL 98
           AR LH  K S    P+ L+GS KGHNIEG HNV+ +L+RYGYLS+NV      NAFDD+L
Sbjct: 2   ARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNV--ETKGNAFDDNL 61

Query: 99  EYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDVFDDKIVNATGSRR------AIGT 158
           E+A+K YQKFF LNVSGILDA+TLHLMSQPRC VPDVF    +N T   R      +IG 
Sbjct: 62  EFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVF----INETSGDRDRDWDHSIGL 121

Query: 159 LYTFF-PGEPKW--SREKLKYAFVNDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVAD 218
            YTFF   +PKW  S+  LKYAFV +FP  ++  V +A   W+  + F FTE   D   D
Sbjct: 122 DYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVTGFTFTEGSADN-TD 181

Query: 219 IRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFNSMMSWSNGAVDETFDVRSVAMHE 278
           ++ISFE+GDHGDG+PF  GSGVLAHAFAPE+GRLH+N+  +W++GA  + +DV +VA+HE
Sbjct: 182 VKISFEVGDHGDGYPFEEGSGVLAHAFAPEDGRLHYNAREAWADGAYPDKYDVGTVALHE 241

Query: 279 LGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGLNELYGNY 314
           LGH LGLGHS  + A+M+P  PP  V  ++ DD++G+  LY  +
Sbjct: 242 LGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNGF 278

[Close]

BLAST of Lcy09g004300 vs. NCBI nr
Match: XP_023544311.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.1 bits (845), Expect = 2.1e-86
Identity = 172/307 (56.03%), Postives = 214/307 (69.71%), Query Frame = 0


Query: 17  MDFKLSLQ-LFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD K  LQ +F+L+A +APHAI AR LH  K S    P+ L+GS KGHNIEG HNV+ +L
Sbjct: 1   MDLKSCLQVMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      NAFDD+LE+A+K YQKFF LNVSGILDA+TL LMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--DTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 137 FDDKIVNATGS------RRAIGTLYTFF-PGEPKWSREK--LKYAFVNDFPSYFEEPVNQ 196
           F    +N TG          IG  Y FF   +PKWS  K  LKY FV  FP  ++  V Q
Sbjct: 121 F----INETGGDGNGDRDHPIGLHYRFFNDSKPKWSASKYNLKYTFVRSFPEQYKSTVVQ 180

Query: 197 ALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFN 256
           A+  W+L S F F+E   D   D+RISFE+G+HGDGHPF  GSGVLAHAFAPE+GRLHFN
Sbjct: 181 AMISWSLNSVFAFSEGSTDD-TDVRISFEVGEHGDGHPFKKGSGVLAHAFAPEDGRLHFN 240

Query: 257 SMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGL 314
              +W++GA  + +DV SVA+HELGH LGLGHS  + A+M+P++ P T KE++ DD+ G+
Sbjct: 241 EWHAWADGAYPDKYDVGSVALHELGHILGLGHSDIRDAVMWPSMRPNTKKELSSDDVSGI 300

[Close]

BLAST of Lcy09g004300 vs. NCBI nr
Match: XP_023544665.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 325.1 bits (832), Expect = 6.7e-85
Identity = 164/301 (54.49%), Postives = 213/301 (70.76%), Query Frame = 0


Query: 17  MDFKLSLQ-LFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD    LQ +F+L+A +APHAI AR LH  K S    P+ L+GS KGHNIEG HNV+ +L
Sbjct: 1   MDLTSCLQVMFVLVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      NAFDD+LE+A+K YQKFF LNVSGILDA+TLHLMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--ETKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDV 120

Query: 137 FDDKIVNATGSRRAIGTLYTFF-PGEPKW--SREKLKYAFVNDFPSYFEEPVNQALKDWA 196
           F ++         +IG  YTFF   +PKW  S+  LKYAFV +FP  ++  V +A   W+
Sbjct: 121 FINETSGEGDRDHSIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWS 180

Query: 197 LYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFNSMMSWS 256
             + F FTE   D   D++ISFE+GDHGDG+PF +GSGVLAHAFAPE+GRLH+N+  +W+
Sbjct: 181 SVTGFTFTEGSADN-TDVKISFEVGDHGDGYPFEDGSGVLAHAFAPEDGRLHYNAQEAWA 240

Query: 257 NGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGLNELYGN 314
           +GA  + +DV +VA+HELGH LGLGHS  + A+M+P  PP  V  ++ DD++G+  LY  
Sbjct: 241 DGAYPDKYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNG 298

[Close]

BLAST of Lcy09g004300 vs. NCBI nr
Match: KAG6602464.1 (hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.6 bits (828), Expect = 2.0e-84
Identity = 168/307 (54.72%), Postives = 213/307 (69.38%), Query Frame = 0


Query: 17  MDFKLSLQ-LFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD K  LQ +F+L+A +APHAI AR LH  K S    P+ L+GS KGHN+EG HNV+ YL
Sbjct: 1   MDLKSCLQVMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAYL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      NAFDD+LE+A+K YQKFF LNVSGILDA+TL LMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--DTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 137 FDDKIVNATGS------RRAIGTLYTFF-PGEPKW--SREKLKYAFVNDFPSYFEEPVNQ 196
           F     N TG         +IG  YTFF   +PKW  S+  LKYAFV +FP  ++  V +
Sbjct: 121 FS----NETGGGGDGDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVRE 180

Query: 197 ALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFN 256
           A   W+  + F FTE   D   D++ISFE+GDHGDG+PF+ GSGVLAHAFAPE+GRLHFN
Sbjct: 181 ATNTWSSVTGFTFTEGSADN-TDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFN 240

Query: 257 SMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGL 314
           +  +W++GA    +DV +VA+HELGH LGLGHS  + A+M+P  PP  V  ++ DD++G+
Sbjct: 241 TRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPYTPPNFVTGLDNDDVNGI 300

[Close]

BLAST of Lcy09g004300 vs. NCBI nr
Match: XP_022959850.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 3.3e-84
Identity = 167/304 (54.93%), Postives = 212/304 (69.74%), Query Frame = 0


Query: 17  MDFKLSLQ-LFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD +  LQ +F+L+A +APHAI AR LHI K S    P+ L+GS KGHNIEG HNV+ +L
Sbjct: 1   MDLRSCLQVMFILVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      N FDD+LE+A+K YQKFF LNVSGILDA+TL LMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--ETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 137 FDDKIVNATGS------RRAIGTLYTFFPG-EPKWSREK--LKYAFVNDFPSYFEEPVNQ 196
           F    +N TG          IG  Y+FF   +PKWS  K  LKY F+  FP  ++  V Q
Sbjct: 121 F----INETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQ 180

Query: 197 ALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFN 256
           A+  W+  S F F+E   D   D+RISFE+GDHGDG PF + +G LAHAFAPE+GRLHFN
Sbjct: 181 AMISWSFNSVFAFSEGSTDN-TDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFN 240

Query: 257 SMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGL 311
           +  +W++GA  + FDV SVA+HELGH LGLGHS  + A+M+P++PP   KE++ DD+ G+
Sbjct: 241 AQHAWADGAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGI 297

[Close]

BLAST of Lcy09g004300 vs. NCBI nr
Match: KAG6602458.1 (hypothetical protein SDJN03_07691, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 321.6 bits (823), Expect = 7.4e-84
Identity = 167/304 (54.93%), Postives = 211/304 (69.41%), Query Frame = 0


Query: 17  MDFKLSLQ-LFLLLALVAPHAISARDLHIRKSSRFSLPRQLLGSRKGHNIEGTHNVKTYL 76
           MD +  LQ +F L+A +APHAI AR LHI K S    P+ L+GS KGHNIEG HNV+ +L
Sbjct: 1   MDLRSCLQVMFTLVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 77  RRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCSVPDV 136
           +RYGYLS+NV      N FDD+LE+A+K YQKFF LNVSGILDA+TL LMSQPRC VPDV
Sbjct: 61  QRYGYLSTNV--ETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 137 FDDKIVNATGS------RRAIGTLYTFFPG-EPKWSREK--LKYAFVNDFPSYFEEPVNQ 196
           F    +N TG          IG  Y+FF   +PKWS  K  LKY F+  FP  ++  V Q
Sbjct: 121 F----INETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVVQ 180

Query: 197 ALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGRLHFN 256
           A+  W+  S F F+E   D   D+RISFE+GDHGDG PF + +G LAHAFAPE+GRLHFN
Sbjct: 181 AMISWSFNSVFAFSEGSTDN-TDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFN 240

Query: 257 SMMSWSNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVKEINFDDIDGL 311
           +  +W++GA  + FDV SVA+HELGH LGLGHS  + A+M+P++PP   KE++ DD+ G+
Sbjct: 241 AQHAWADGAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGI 297

[Close]

BLAST of Lcy09g004300 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.1e-48
Identity = 109/271 (40.22%), Postives = 145/271 (53.51%), Query Frame = 0


Query: 55  QLLGSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVS 114
           +L G   G NI G   +K Y RR+GY+++     N  + FDD L+ A+  YQK F L V+
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYITTT---GNCTDDFDDVLQSAINTYQKNFNLKVT 106

Query: 115 GILDADTLHLMSQPRCSVPDVFDDKIVNATGSRRAIGTLYTFFPGEPKWSREK--LKYAF 174
           G LD+ TL  + +PRC  PD+ D       G        Y+FFPG+P+W + K  L YAF
Sbjct: 107 GKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAF 166

Query: 175 V--NDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSG 234
              N+     +   ++A   WA  +P  FT  +    ADI I F  G+HGDG PF    G
Sbjct: 167 APQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG 226

Query: 235 VLAHAFAPENGRLHFNSMMSW--SNGAVDE-------TFDVRSVAMHELGHNLGLGHSTS 294
            LAHA +P  G LH +    W  SNG +           D+ SVA+HE+GH LGLGHS+ 
Sbjct: 227 TLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 286

Query: 295 KQAIMFPTIPPATVK-EINFDDIDGLNELYG 312
           + AIMFP I     K E+  DDI+G+  LYG
Sbjct: 287 EDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lcy09g004300 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.7e-47
Identity = 114/315 (36.19%), Postives = 166/315 (52.70%), Query Frame = 0


Query: 26  FLLLALVAPHAISARD--------------LHIRKSSRFSLPRQLLGSRKGHNIEGTHNV 85
           F+L+    P+   AR+              +H+  S+     R L+  + G ++ G   +
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSR-LVDVQIGSHVSGVSEL 74

Query: 86  KTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRCS 145
           K YL R+GY++    E  +D  FD  LE A+  YQ+   L ++G LD  T+ LMS PRC 
Sbjct: 75  KRYLHRFGYVNDG-SEIFSD-VFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCG 134

Query: 146 VPD---VFDDKIVNATGSRRAIGTLYTFFPGEPKWSREKLKYAF--VNDFPSYFEEPV-- 205
           V D     ++  ++ T         YT+F G+PKW+R+ L YA    +       E V  
Sbjct: 135 VSDTHMTINNDFLHTTAH-------YTYFNGKPKWNRDTLTYAISKTHKLDYLTSEDVKT 194

Query: 206 --NQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENGR 265
              +A   W+   P  F EVD    AD++I F  GDHGDG PF    G LAHAFAPENGR
Sbjct: 195 VFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGR 254

Query: 266 LHFNSMMSW-----SNGAVDETFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVK- 312
           LH ++  +W       G+ +   D+ SVA HE+GH LGLGHS+ + A+M+P++ P T K 
Sbjct: 255 LHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKV 314

[Close]

BLAST of Lcy09g004300 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.1e-45
Identity = 103/255 (40.39%), Postives = 143/255 (56.08%), Query Frame = 0


Query: 71  VKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGILDADTLHLMSQPRC 130
           +K +L++YGYL  N  + ++D +F    E A+ RYQK   L ++G  D+DTL  +  PRC
Sbjct: 53  IKRHLQQYGYLPQN--KESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQILLPRC 112

Query: 131 SVPDVFDDKIVNATGSRRAIGTLYTFFPGEPKWSRE---KLKYAFVND------FPSYFE 190
             PD  + K      +    G  Y +FPG P+W+R+   KL YAF  +       P+   
Sbjct: 113 GFPDDVEPKT-----APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIR 172

Query: 191 EPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDGHPFINGSGVLAHAFAPENG 250
               +A   WA   P  F E +   +ADI+I F  GDHGDG PF    GVLAH F+PENG
Sbjct: 173 RVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENG 232

Query: 251 RLHFNSMMSWSNGAVDE----TFDVRSVAMHELGHNLGLGHSTSKQAIMFPTIPPATVK- 310
           RLH +   +W+    +E      D+ SVA+HE+GH LGLGHS+ K A M+PT+ P + K 
Sbjct: 233 RLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKV 292

Query: 311 EINFDDIDGLNELYG 312
            +N DD+ G+  LYG
Sbjct: 293 NLNMDDVVGVQSLYG 296

[Close]

BLAST of Lcy09g004300 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 179.9 bits (455), Expect = 3.2e-45
Identity = 103/277 (37.18%), Postives = 149/277 (53.79%), Query Frame = 0


Query: 58  GSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGIL 117
           G   G N++G + +K Y +R+GY+       N  + FDD L+ AV+ YQ  F LNV+G L
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYI-PETFSGNFTDDFDDILKAAVELYQTNFNLNVTGEL 108

Query: 118 DADTLHLMSQPRCSVPDVFDDKIVNATGSRRAIGT-----------LYTFFPGEPKWSRE 177
           DA T+  +  PRC  PDV +   +   G R+                YT FPGEP+W R 
Sbjct: 109 DALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRN 168

Query: 178 K--LKYAF--VNDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGDG 237
           +  L YAF   N      +   ++A   W+  +   FT  +  + +DI I F  GDHGDG
Sbjct: 169 RRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDG 228

Query: 238 HPFINGSGVLAHAFAPENGRLHFNSMMSW-------SNGAVDETFDVRSVAMHELGHNLG 297
            PF    G LAHAF+P +G+ H ++  +W       S  +V    D+ SVA+HE+GH LG
Sbjct: 229 EPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLG 288

Query: 298 LGHSTSKQAIMFPTIPPATVK-EINFDDIDGLNELYG 312
           LGHS+ +++IM+PTI     K ++  DD++G+  LYG
Sbjct: 289 LGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Lcy09g004300 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.2e-44
Identity = 102/279 (36.56%), Postives = 148/279 (53.05%), Query Frame = 0


Query: 58  GSRKGHNIEGTHNVKTYLRRYGYLSSNVVEANNDNAFDDDLEYAVKRYQKFFKLNVSGIL 117
           G   G   +G + +K Y + +GY++   +  N  + FDD L+ AV+ YQ+ F+LNV+G+L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 118 DADTLHLMSQPRCSVPDVFD------------DKIVNATGSRRAIGTLYTFFPGEPKWSR 177
           D  TL  +  PRC  PDV +            +      G R      Y+FFPGEP+W R
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 178 EK--LKYAF--VNDFPSYFEEPVNQALKDWALYSPFEFTEVDHDAVADIRISFELGDHGD 237
            +  L YAF   N      +   ++A   W   +P  FT V+  + +DI I F  G+HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 238 GHPFINGSGVLAHAFAPENGRLHFNSMMSW------SNG--AVDETFDVRSVAMHELGHN 297
           G PF      LAHAF+P  G  H +   +W       +G  +V E  D+ SVA+HE+GH 
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 298 LGLGHSTSKQAIMFPTIPPATVK-EINFDDIDGLNELYG 312
           LGLGHS+ + +IM+PTI     K ++  DD++G+  LYG
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

The following BLAST results are available for this feature:

BLAST of Lcy09g004300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUJ5	5.8e-47	40.22	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O23507	3.7e-46	36.19	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
P29136	7.1e-45	39.92	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q8GWW6	1.6e-44	40.39	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]
O04529	4.6e-44	37.18	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]

BLAST of Lcy09g004300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7I1	1.6e-84	54.93	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 ...	[more]
A0A6J1H7Q4	1.7e-81	53.82	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 ...	[more]
A0A0A0KX75	2.8e-81	53.87	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632100 PE=3 S...	[more]
A0A6J1BVR3	4.1e-80	53.25	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3...	[more]
A0A6J1H5Y2	2.7e-79	54.58	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 ...	[more]

BLAST of Lcy09g004300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023544311.1	2.1e-86	56.03	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023544665.1	6.7e-85	54.49	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602464.1	2.0e-84	54.72	hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022959850.1	3.3e-84	54.93	metalloendoproteinase 1-like [Cucurbita moschata]	[more]
KAG6602458.1	7.4e-84	54.93	hypothetical protein SDJN03_07691, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lcy09g004300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59970.1	4.1e-48	40.22	Matrixin family protein	[more]
AT4G16640.1	2.7e-47	36.19	Matrixin family protein	[more]
AT2G45040.1	1.1e-45	40.39	Matrixin family protein	[more]
AT1G70170.1	3.2e-45	37.18	matrix metalloproteinase	[more]
AT1G24140.1	1.2e-44	36.56	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 265..290 score: 57.36 coord: 298..311 score: 45.03 coord: 125..138 score: 52.7 coord: 208..236 score: 55.05 coord: 184..199 score: 30.34
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 159..312 e-value: 3.1E-27 score: 106.5
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 59..313 e-value: 8.1E-71 score: 240.3
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 66..125 e-value: 4.0E-14 score: 52.6
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 162..311 e-value: 8.9E-37 score: 126.4
None	No IPR available	PIRSR	PIRSR001191-1	PIRSR001191-1	coord: 68..311 e-value: 8.4E-56 score: 187.4
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 39..312
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 39..312
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 156..312
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 162..311 e-value: 1.04539E-46 score: 153.129
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 62..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g004300.1	Lcy09g004300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity