Lcy09g003880 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g003880
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionprotease Do-like 8, chloroplastic
LocationChr09: 3390669 .. 3396093 (+)
RNA-Seq ExpressionLcy09g003880
SyntenyLcy09g003880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCACAATGTACTAAGCTCTCAGTTCTCACTGGTCTAAAACTTATCTCAATTCATCCATTCTTTTCTAAAAGCACAATAAAACCATAATCCCAAAAGAGAGACCAATCCCTTTTTTATTAGAGACAGAATTGGTTGATCCTTGGTGGTTGGTTATGGAGCATGTAAGCTTCGAAGTTCCATAGCTCCCATTACTGGGTGAAGCTCTTGCCCCTTCAACTTCATTGCCTCTCAATCTCCATCTATCATCGCAGGCCCTTTCTCTCTACTGGGTTGTTGGATTACAACTCCAGTCATCGTCTCTCTCAGTTTTGCCTCTCTCTTTGTCCCTGATTTTGCCGAATCCGTTGAGAGTTCATCTCCAGGTTAGTAATTAATCTTAATGGAGGCGTTTTACAATCTCCCTCTTCTTTTGTACTGATTCGATGACGATATTTGAGTGAGAAACCATTTTCAAAATTGTGAAATTAGTCTTGCTCCTTGGTATGATTTGTTTGTTTCTAAATTTAGAAATGCAAGTTCTGGCCTGCAATGCTCCCCACCTGCGAACAATGGTAAAATCTGGTGTTTCGACTTCGACCCATTTCTTGGGAAGGCGGGAACTGTGCTTTGATGGTGTGTCTTCTGTATGTTCTCCTTCTCAACCCTCCACTTCAAATCCCAGCACTGCCCAATCCATTTCCATTCAAAGGTTGTCTTTCTCTCTTTTTTACTCCGTGCCTCTGCATTGTCTGATCAAAATTATTTGTTCATTGTTTACCCATTGATATGCATTTATATGTTTTAGTTATGGATAGTGTTGTTGGTTGTTGGTATTGGATTTTGTGTGAAGTTTGTAATTTGGTTAGCTGATTATTTAGGAGTGCTGGTGATGACTCTGGGGATCTAGGTTCTGTACTGAGCAGCAAATTTCTTTTCACAACTCGGCGGACGTTATTTGCTAGTTTGTTTCTGAGTTCTTGGGCATATCCTTCAAGGTACTTCTTTCACTGTTTCAGCTTTATGCTCTGCTCAATGAAGTGCATTTGGTTAAAGATTGGTCTCGTCTTCTTATATTATTTTCTGTCATGTAAGAAATGTTTTTTTTGGATATCCAAGAGTGTCCAGGCTAGTTTGCACGCTCCTTGACATACATTTTCATAGAGAAATGTTGAGTTGATTGACACGCATTTTCAATTTCTAATGTCATTTTTGTAAGTGAAAATAAAATTGTGGTGAAGTAGCAAATTTGTCAAGTTTCAAAGCTGAAACCATGTATAAATCTTCCCTTCAGCATGAAATGTGCAAATTTCTGATCGAGGGTTCTAAGAAGCATAATTGTTAGTTTAAAGAGTAATACTTATAACTTTTTATATGTTCATACTTTGTTACACTTATGAGAAAATAACTTATTTATAACCAAGTTGTGTAGTGTATTTACAGCTCAAGCTTTGGGCGATCCATCTGTAACCATAGAGGAAGTAACTCCCACTATTTCACCTTCTGGATCCCTTTTCCCTACAGAGGTTTTTTTTTGTTTTGTTCTTCTCACTCTATACTTATTTTTGCTTACTTCCTAATAGATTTGCTTCGGAACTTCGTTGCATTGTTGTTATTTATCATGGTGTATGAATGGGAAATATGTGATTGACAGGAAAGAATTGTCCAATTGTTCGAGAAAAATACATACTCTGTTGTCAATATTTTTGATGTGACTTTGCGTCCTCAACTCAATGTAACTGGCATGGTTGAGGTAATCTAGCACTAGCAATAAAACTATGGCTCATGTTGTTTTAGCAACTTTGACACATTACAAATAGACATATTTCAAGCCGTTTAATGTGTTCCTATTTTGATAACCATCATATTAGATCATCATTTTGTAATCATAGCCATGCCTGTTCACAGATTCCTGAAGGAAATGGTTCTGGAGTAGTTTGGGATGATCAAGGACATATAGTAACAAACTATCATGGTGAGAGCTTTTAACGGTCCCAACATACAGCTAAAACAACTACATTGAATGACTTTAGAAAGGATAATCTAAATCTGGAAACGTTGTTTTACCAAAATATGGCATTTCTAATGCCAAGCCTAGTATTTTACCTAATCAACTTTAGACTGTGATTCAAAAAGAAGTTCAACATTTCCTCATAAGCTTTGTTTTTTTTCTAATCACTGAACAGTAGTTGCTAGTGCCCTTGCAAGAAATCCGAGCTCGGGGCAGGTTGTTGCACGAGTTAATATTCTAGCATCTGATGGGTGAGAATACTTGAAGTTAGACCAATATACTATTGTGATAAACAACAGGTATCTGTTTCCTCAATTTTTCTACGTCTTTGAATAGGATACAGAAGAATTTTGAAGGCAAGCTCATTGGTGCAGACCGTACAAAGGATCTTGCTGTTTTGAAGGCAAGGCTTTTCAACTTTAAATTCTTTTGCTTTATAGGTTATCCAAGAATTTGCACTTTTAAATGATATGCTTTTATTTCATTTAATTTCAAATGTCAACGATTGATGAACAATTTTAGGTTTATTAGTTCAAGCATGCATCATTACCATTGTTACAAAAAAAAAAAAAAATAAAAAATAAAAAAAAATAAAAATAAGAAAACAAAAAATAAAAAATATCACTGGTTGTGAATTCTGAGACTATTGCTCATCCATTCTCTGATTTGTCTCACCCTCGAAAGTCTATGCTTGTTCTTTGTTCTTTCATATTCTAAATGAAATTTGGTTTCTTATATATATATAAAAAATCCCTCCAAAGTCTAACCGAAGATGAAATAATCTGTGTCCTTGCAGGTGGATGCCTCAGACGATTTATTGAGACCAATCAAGGTAGGGCAGTCATCTTCTCTTAAAGTGGGGCAACAGTGCTTGGCAATTGGAAATCCTTTTGGTTTCGATCATACTCTCACAGTCGGAGTCATTAGTGGACTAAATCGCGATATTTTTAGTCAGACTGGCGCCACCATTGGTGGTGGAATCCAAACAGATGCAGCTATAAATCCTGGGAATAGGTTAATCTAAGATTCATACAAAAATGAATTGTACCCCACTTTATCATTCTTTGTTCAGGAACTCTCCTGTCTTATTATTCATTTCTTTGATTGCTGAATGTATTACCACCATATATACGTAACTTTGACATCAAGGACAAGGAGCTAAAATTTTCCTGTCACTTTGACAGTGGAGGTCCTCTACTTGATTCAAAAGGAAACTTGATTGGTATCAATACTGCTATTTTTACTCAAACAGGTAACGTCCATCTTTCTTCTGCCAAGTTCTTCACCATCAATGCTTAATGCAATATTTACAACACTCAGTTGCATCCATGCTGTAACGACGTGGCAGTTGTCGTGATTTTCTTATTTGCCTCACTCCATGCTTCAACAATAACATTAGGAATGTGATGAATATTGGTCTTACTATTATGGTTGCAGGAACTTCAGCTGGTGTTGGTTTTGCAATCCCATCTTCAACCGTAGTCAAGATTGTACCTCAGTTAATCCAATTTGGTAGAGTAAGTGATCTTGCTTTTTACCAATTGTGGTGTAGGATTAATTTGGTGTTTATACAAGACTTATCTTATTTCTATTCGATTTATGTGTATATATATATTTGTTTCCGAAGCCAGCCTATATTTTTAGTTAGTTATACCAGCTTAGTTCTTTTAACACTCGGATTCTGTCTATTCTTTATACAGTATAAACTCTTACAAAGGCTTTAAACCATTCTTGATTAAGATTCTGGTTTTTCATATAGGTTGTTCGGGCTGGCATAAATGTAGATTTTGCTCCCGACCTGATTGCAAATCAACTAAATGTCCGTGATGGAGCTCTAATCCTGCAGGTTGATTCTCCCCAGACTAATTTCTTCTTTTTATATTTTGTGCTCTTTTCTTGCTAACATGCATCACAATTAGCCAATTTAAGATGATTTATAGTCTTTTTATCTATGGTTTTTACTCCTCAGGTTCCTGCAAATAGTCCTGCAGCCAAGGCTGGACTATTGCCCACCACCAGAGGATTTGCAGGTAATATAGTACTCGGGGACATCATTGCTGCCATTGATAACAAGCCGGTGAGCTCTTCTTTTCAAACTAACGTTGCATTTAGCAGCCACTGCATTAAACTGGGAAAACATTTCTGTATAATTGCATCAACAATGAAAAGTCCTTCCAGATATTTAAGAAAGTTAGCAGTGCTTCTTGAGAATATAAGGTTCTGCATCTCTCTATAGGTCAAAAACAAAGCAGAATTGTACAAGTTATTGGATGAGTACGAGGCCGGAGACAAAGTTATTCTAAAGATAAAGAGAGGAGGACAAAATTTGGAGCTGCCTCTGATATTAGAAGAAAGTCAGTAGGAATTAGGAAGTATCAGCAGGAAATTGCGAATAACATGCTAACCCATTTCGACATTGTACTCGGAATCGAATGGGGAAGAAGAAGATTAAGAGAAGCAATGAAAGCATAAAGCTTTGAGGTAAAATGAGTAATATTTTGTTATAAAATCGAAGCTTTCTATAGAAAGTTGATGAGTTTCTGTGTGATATTGAAATGTTTTGCATGTTTCAAATCATAACAGCAAGCAGTGAATTTGGTGAGTTTTCATGGGGTGGACTAAACTTTTTCAACTTGAGGCCTTGATGGTAAGGGAGAGATGGAAAATCAACTATGAGCCTTTGTTAGTATTGATGTAAATGTTAATGTAAAGATAAAATGTCAAATTAATCCGATGCTCATAATGGATCTAATTCAAGAGTCAATATGTGCTTCTGTAATCTTTATCTCCCTGTTTATTCTGGAATTATGACATAATTATCATTTGTTATCATCTGGAAGTCGCATGGAGATATTCGTTTTGTTACATTAATATTGAAATATAAACAGGATTTCATCAGTGTTTGAAAATTGATTTATGAGTTATCTTTATAGTTTGGGTGAATCAAACCCACCCAAATGTATGAATGAAAAAAATGTGTTTGTTTTCCATTTTACACATTTTCTTCTAATTAGTAACTTGAAAATTTTGACTTAAATAAAACTTGAGGTTGGCTCAATCCAAAGATCATTGGTTAATTGATTGGTGGGTTACTTCCAAAGATGATCTACCAACAAAAAACTGACTTAAATCAGTGAAATGCATTGAAGTGTAATGTTAAATTTTGGGGAGATTTTTATTTGTCTCCGTGAAAGAATGTCAACACTTTCATATTGATGTTAACAAATGAACAAGTCTTTTCACAACTGGTTTTGTTGTACAGATACAAATTGTGTAATTTCAAGCATTTCTAACTTTAGACTTGTTGAGATTTGTGGAGCTTGTTAATTATTAAGATGACTTAGTCAAGGACTTGTTAGAGGAAGGTGTATTCGATGCCTGGTTGACAAAATGGAGAAAATGAGTAACAAAGCACTATAGCATAAGGCAGACCCAAG

mRNA sequence

TTCACAATGTACTAAGCTCTCAGTTCTCACTGGTCTAAAACTTATCTCAATTCATCCATTCTTTTCTAAAAGCACAATAAAACCATAATCCCAAAAGAGAGACCAATCCCTTTTTTATTAGAGACAGAATTGGTTGATCCTTGGTGGTTGGTTATGGAGCATGTAAGCTTCGAAGTTCCATAGCTCCCATTACTGGGTGAAGCTCTTGCCCCTTCAACTTCATTGCCTCTCAATCTCCATCTATCATCGCAGGCCCTTTCTCTCTACTGGGTTGTTGGATTACAACTCCAGTCATCGTCTCTCTCAGTTTTGCCTCTCTCTTTGTCCCTGATTTTGCCGAATCCGTTGAGAGTTCATCTCCAGAAATGCAAGTTCTGGCCTGCAATGCTCCCCACCTGCGAACAATGGTAAAATCTGGTGTTTCGACTTCGACCCATTTCTTGGGAAGGCGGGAACTGTGCTTTGATGGTGTGTCTTCTGTATGTTCTCCTTCTCAACCCTCCACTTCAAATCCCAGCACTGCCCAATCCATTTCCATTCAAAGGAGTGCTGGTGATGACTCTGGGGATCTAGGTTCTGTACTGAGCAGCAAATTTCTTTTCACAACTCGGCGGACGTTATTTGCTAGTTTGTTTCTGAGTTCTTGGGCATATCCTTCAAGTGTATTTACAGCTCAAGCTTTGGGCGATCCATCTGTAACCATAGAGGAAGTAACTCCCACTATTTCACCTTCTGGATCCCTTTTCCCTACAGAGGAAAGAATTGTCCAATTGTTCGAGAAAAATACATACTCTGTTGTCAATATTTTTGATGTGACTTTGCGTCCTCAACTCAATGTAACTGGCATGGTTGAGATTCCTGAAGGAAATGGTTCTGGAGTAGTTTGGGATGATCAAGGACATATAGTAACAAACTATCATGTAGTTGCTAGTGCCCTTGCAAGAAATCCGAGCTCGGGGCAGGTTGTTGCACGAGTTAATATTCTAGCATCTGATGGGATACAGAAGAATTTTGAAGGCAAGCTCATTGGTGCAGACCGTACAAAGGATCTTGCTGTTTTGAAGGTGGATGCCTCAGACGATTTATTGAGACCAATCAAGGTAGGGCAGTCATCTTCTCTTAAAGTGGGGCAACAGTGCTTGGCAATTGGAAATCCTTTTGGTTTCGATCATACTCTCACAGTCGGAGTCATTAGTGGACTAAATCGCGATATTTTTAGTCAGACTGGCGCCACCATTGGTGGTGGAATCCAAACAGATGCAGCTATAAATCCTGGGAATAGTGGAGGTCCTCTACTTGATTCAAAAGGAAACTTGATTGGTATCAATACTGCTATTTTTACTCAAACAGGAACTTCAGCTGGTGTTGGTTTTGCAATCCCATCTTCAACCGTAGTCAAGATTGTACCTCAGTTAATCCAATTTGGTAGAGTTGTTCGGGCTGGCATAAATGTAGATTTTGCTCCCGACCTGATTGCAAATCAACTAAATGTCCGTGATGGAGCTCTAATCCTGCAGGTTCCTGCAAATAGTCCTGCAGCCAAGGCTGGACTATTGCCCACCACCAGAGGATTTGCAGGTAATATAGTACTCGGGGACATCATTGCTGCCATTGATAACAAGCCGGTCAAAAACAAAGCAGAATTGTACAAGTTATTGGATGAGTACGAGGCCGGAGACAAAGTTATTCTAAAGATAAAGAGAGGAGGACAAAATTTGGAGCTGCCTCTGATATTAGAAGAAAGTCAGTAGGAATTAGGAAGTATCAGCAGGAAATTGCGAATAACATGCTAACCCATTTCGACATTGTACTCGGAATCGAATGGGGAAGAAGAAGATTAAGAGAAGCAATGAAAGCATAAAGCTTTGAGCAAGCAGTGAATTTGGTGAGTTTTCATGGGGTGGACTAAACTTTTTCAACTTGAGGCCTTGATGGTAAGGGAGAGATGGAAAATCAACTATGAGCCTTTGTTAGTATTGATGTAAATGTTAATGTAAAGATAAAATGTCAAATTAATCCGATGCTCATAATGGATCTAATTCAAGAGTCAATATGTGCTTCTGTAATCTTTATCTCCCTGTTTATTCTGGAATTATGACATAATTATCATTTGTTATCATCTGGAAGTCGCATGGAGATATTCGTTTTGTTACATTAATATTGAAATATAAACAGGATTTCATCAGTGTTTGAAAATTGATTTATGAGTTATCTTTATAGTTTGGGTGAATCAAACCCACCCAAATGTATGAATGAAAAAAATGTGTTTGTTTTCCATTTTACACATTTTCTTCTAATTAGTAACTTGAAAATTTTGACTTAAATAAAACTTGAGGTTGGCTCAATCCAAAGATCATTGGTTAATTGATTGGTGGGTTACTTCCAAAGATGATCTACCAACAAAAAACTGACTTAAATCAGTGAAATGCATTGAAGTGTAATGTTAAATTTTGGGGAGATTTTTATTTGTCTCCGTGAAAGAATGTCAACACTTTCATATTGATGTTAACAAATGAACAAGTCTTTTCACAACTGGTTTTGTTGTACAGATACAAATTGTGTAATTTCAAGCATTTCTAACTTTAGACTTGTTGAGATTTGTGGAGCTTGTTAATTATTAAGATGACTTAGTCAAGGACTTGTTAGAGGAAGGTGTATTCGATGCCTGGTTGACAAAATGGAGAAAATGAGTAACAAAGCACTATAGCATAAGGCAGACCCAAG

Coding sequence (CDS)

ATGCAAGTTCTGGCCTGCAATGCTCCCCACCTGCGAACAATGGTAAAATCTGGTGTTTCGACTTCGACCCATTTCTTGGGAAGGCGGGAACTGTGCTTTGATGGTGTGTCTTCTGTATGTTCTCCTTCTCAACCCTCCACTTCAAATCCCAGCACTGCCCAATCCATTTCCATTCAAAGGAGTGCTGGTGATGACTCTGGGGATCTAGGTTCTGTACTGAGCAGCAAATTTCTTTTCACAACTCGGCGGACGTTATTTGCTAGTTTGTTTCTGAGTTCTTGGGCATATCCTTCAAGTGTATTTACAGCTCAAGCTTTGGGCGATCCATCTGTAACCATAGAGGAAGTAACTCCCACTATTTCACCTTCTGGATCCCTTTTCCCTACAGAGGAAAGAATTGTCCAATTGTTCGAGAAAAATACATACTCTGTTGTCAATATTTTTGATGTGACTTTGCGTCCTCAACTCAATGTAACTGGCATGGTTGAGATTCCTGAAGGAAATGGTTCTGGAGTAGTTTGGGATGATCAAGGACATATAGTAACAAACTATCATGTAGTTGCTAGTGCCCTTGCAAGAAATCCGAGCTCGGGGCAGGTTGTTGCACGAGTTAATATTCTAGCATCTGATGGGATACAGAAGAATTTTGAAGGCAAGCTCATTGGTGCAGACCGTACAAAGGATCTTGCTGTTTTGAAGGTGGATGCCTCAGACGATTTATTGAGACCAATCAAGGTAGGGCAGTCATCTTCTCTTAAAGTGGGGCAACAGTGCTTGGCAATTGGAAATCCTTTTGGTTTCGATCATACTCTCACAGTCGGAGTCATTAGTGGACTAAATCGCGATATTTTTAGTCAGACTGGCGCCACCATTGGTGGTGGAATCCAAACAGATGCAGCTATAAATCCTGGGAATAGTGGAGGTCCTCTACTTGATTCAAAAGGAAACTTGATTGGTATCAATACTGCTATTTTTACTCAAACAGGAACTTCAGCTGGTGTTGGTTTTGCAATCCCATCTTCAACCGTAGTCAAGATTGTACCTCAGTTAATCCAATTTGGTAGAGTTGTTCGGGCTGGCATAAATGTAGATTTTGCTCCCGACCTGATTGCAAATCAACTAAATGTCCGTGATGGAGCTCTAATCCTGCAGGTTCCTGCAAATAGTCCTGCAGCCAAGGCTGGACTATTGCCCACCACCAGAGGATTTGCAGGTAATATAGTACTCGGGGACATCATTGCTGCCATTGATAACAAGCCGGTCAAAAACAAAGCAGAATTGTACAAGTTATTGGATGAGTACGAGGCCGGAGACAAAGTTATTCTAAAGATAAAGAGAGGAGGACAAAATTTGGAGCTGCCTCTGATATTAGAAGAAAGTCAGTAG

Protein sequence

MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQRSAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTISPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAGINVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ
Homology
BLAST of Lcy09g003880 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.8e-172
Identity = 327/463 (70.63%), Postives = 373/463 (80.56%), Query Frame = 0

Query: 1   MQVLA--CNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSP-SQPSTSNPSTAQSIS 60
           MQV+A  C+ P+              F+GRR+L    +SSVCS  SQ    +     S+ 
Sbjct: 1   MQVIASFCSKPN-----------ENEFVGRRQL----LSSVCSKISQGDVVSHPPVSSVK 60

Query: 61  IQRSAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSV-TIEEV 120
           + +   D   +L  +       TTRR L  SLF++    PS   +A ALGDPSV T+E+V
Sbjct: 61  VTQ---DWKSNLHELAVKSVPSTTRRILLTSLFMNLCFNPSRYLSALALGDPSVATVEDV 120

Query: 121 TPTISPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDD 180
           +PT+ P+G LFPTE RIVQLFEKNTYSVVNIFDVTLRPQL +TG+VEIPEGNGSGVVWD 
Sbjct: 121 SPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDG 180

Query: 181 QGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDA 240
           QG+IVTNYHV+ +AL+RNPS G VV RVNILASDG+QKNFEGKL+GADR KDLAVLKVDA
Sbjct: 181 QGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDA 240

Query: 241 SDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQ 300
            + LL+PIKVGQS+SLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQ
Sbjct: 241 PETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQ 300

Query: 301 TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRV 360
           TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTV+KIVPQLIQF +V
Sbjct: 301 TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKV 360

Query: 361 VRAGINVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAI 420
           +RAGIN++ APD +ANQLNVR+GAL+LQVP  S A KAGL PT+RGFAGNIVLGDII A+
Sbjct: 361 LRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAV 420

Query: 421 DNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEE 460
           D+KPVKNKAEL K+LDEY  GDKV LKIKRG ++LEL + LEE
Sbjct: 421 DDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEE 445

BLAST of Lcy09g003880 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-80
Identity = 170/363 (46.83%), Postives = 234/363 (64.46%), Query Frame = 0

Query: 98  SSVFTAQALGDPSVTIEEVTPTISPSGSLFPTEE-RIVQLFEKNTYSVVNIFDVTLRPQL 157
           +SV  + +L   S  +E  +  +  +     T+E   V+LF++NT SVV I ++ +R   
Sbjct: 85  TSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVRLFQENTPSVVYITNLAVRQDA 144

Query: 158 NVTGMVEIPEGNGSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNF 217
               ++E+P+G+GSG VWD QGHIVTNYHV+  A           + + +  +D  Q  F
Sbjct: 145 FTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGA-----------SDLRVTLAD--QTTF 204

Query: 218 EGKLIGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVI 277
           + K++G D+ KD+AVL++DA  + LRPI VG S+ L VGQ+  AIGNPFG DHTLT GVI
Sbjct: 205 DAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTLTTGVI 264

Query: 278 SGLNRDIFS-QTGATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVG 337
           SGL R+I S  TG  I   IQTDAAINPGNSGGPLLDS G LIGINTAI++ +G S+GVG
Sbjct: 265 SGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVG 324

Query: 338 FAIPSSTVVKIVPQLIQFGRVVRAGINVDFAPDLIANQLNVRDGALILQVPANSPAAKAG 397
           F+IP  TV  IV QL++FG+V R  + + FAPD    QL V  G L+L  P + PA KAG
Sbjct: 325 FSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGV-SGVLVLDAPPSGPAGKAG 384

Query: 398 LLPTTRGFAGNIVLGDIIAAIDNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPL 457
           L  T R   G +VLGDII +++   V N ++LY++LD+ + GD+V +++ RG    ++ +
Sbjct: 385 LQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEVLRGDHKEKISV 433

Query: 458 ILE 459
            LE
Sbjct: 445 TLE 433

BLAST of Lcy09g003880 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 199.5 bits (506), Expect = 8.2e-50
Identity = 122/276 (44.20%), Postives = 164/276 (59.42%), Query Frame = 0

Query: 89  LFLSSWAYPSSVFTAQALGDPSVTIEEVTPTISPSGSLFPTEERIVQLFEKNTYSVVNIF 148
           +F SS A  SS+  +     P  +   +         L   EER V LF+K + SVV I 
Sbjct: 47  IFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSVVYIE 106

Query: 149 DVTLRPQLNVTGMVEIP----EGNGSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARV 208
            + L    +   + +      EG GSG VWD  GHIVTNYHV+A         G    +V
Sbjct: 107 AIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAK--LATDQFGLQRCKV 166

Query: 209 NILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNP 268
           +++ + G + + EGK++G D   DLAVLK++     L P+ +G S+ L+VGQ C AIGNP
Sbjct: 167 SLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNP 226

Query: 269 FGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAI 328
           +G+++TLT+GV+SGL R+I S  G +I   IQTDA IN GNSGGPLLDS G+ IG+NTA 
Sbjct: 227 YGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSGGPLLDSYGHTIGVNTAT 286

Query: 329 FTQ--TGTSAGVGFAIPSSTVVKIVPQLIQFGRVVR 359
           FT+  +G S+GV FAIP  TVV+ VP LI +G   R
Sbjct: 287 FTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYR 320

BLAST of Lcy09g003880 vs. ExPASy Swiss-Prot
Match: F6AA62 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fulva (strain 12-X) OX=743720 GN=Psefu_3239 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.7e-43
Identity = 115/289 (39.79%), Postives = 164/289 (56.75%), Query Frame = 0

Query: 169 GSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKD 228
           GSG +    G+I+TN HVVA A        +++ R+    SD  +   E KLIG D   D
Sbjct: 102 GSGFIISKDGYILTNNHVVADA-------DEIIVRL----SD--RSELEAKLIGTDPRSD 161

Query: 229 LAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG 288
           +A+LKV+A+D  L  +K+G S +LKVG+  LAIG+PFGFDH++T G++S   R + +++ 
Sbjct: 162 VALLKVEAND--LPTVKLGNSDNLKVGEWVLAIGSPFGFDHSVTAGIVSAKGRSLPNESY 221

Query: 289 ATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVP 348
                 IQTD AINPGNSGGPL +  G ++GIN+ IFT++G   G+ FAIP S  + +  
Sbjct: 222 VPF---IQTDVAINPGNSGGPLFNLDGEVVGINSQIFTRSGGFMGLSFAIPMSVAMDVAD 281

Query: 349 QLIQFGRVVRAGINV---DFAPDLIAN-QLNVRDGALILQVPANSPAAKAGLLPTTRGFA 408
           QL   G+V R  + V   +   DL  +  L    GAL+ QV  + PAAK GL        
Sbjct: 282 QLKASGKVSRGWLGVVIQEVNKDLAESFGLEKPAGALVAQVLEDGPAAKGGL-------- 341

Query: 409 GNIVLGDIIAAIDNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLEL 454
               +GD+I ++D KP+   A+L  L+   + G K  L+I R G    L
Sbjct: 342 ---QVGDVILSLDGKPIIMSADLPHLVGALKPGTKANLEIVREGSRKTL 361

BLAST of Lcy09g003880 vs. ExPASy Swiss-Prot
Match: O05942 (Probable periplasmic serine endoprotease DegP-like OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=htrA PE=3 SV=2)

HSP 1 Score: 173.7 bits (439), Expect = 4.8e-42
Identity = 119/307 (38.76%), Postives = 171/307 (55.70%), Query Frame = 0

Query: 156 LNVTGMVEIPEG--NGSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQ 215
           LN+  + + P+    GSG +    G IVTNYHV+A+           V ++NI  +D  +
Sbjct: 108 LNLEEIAKTPKSIPLGSGFIIAPNGLIVTNYHVIAN-----------VEKINIKLADNTE 167

Query: 216 KNFEGKLIGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNPFG-FDHTLT 275
             F  KLIG+D   DLA+LK+D S++ L  ++ G S+  +VG   +AIGNPFG    T+T
Sbjct: 168 --FLAKLIGSDSKTDLALLKID-SEEPLPFVEFGDSNDARVGDWVIAIGNPFGNLGGTVT 227

Query: 276 VGVISGLNRDIFSQTGATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSA 335
            G+IS   RDI   T   +   IQTDAAIN GNSGGP+ +    +IG+NTAIF+  GT+ 
Sbjct: 228 SGIISSKGRDIDVDTDNIVDNFIQTDAAINNGNSGGPMFNLDQKVIGVNTAIFSPLGTNI 287

Query: 336 GVGFAIPSSTVVKIVPQLIQFGRVVRA--GINVDFAPDLIANQLNVR--DGALILQVPAN 395
           G+GFAIPS+T   I+ +L + G+V R   G+ +    + I+  L  +  +G L+ +V  N
Sbjct: 288 GIGFAIPSNTAKPIIERLKKDGKVSRGRLGVTIQDLTEEISEVLGFKGTNGVLVSKVQEN 347

Query: 396 SPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKPVKNKAELYKLLDEYEAGDKVILKIKRGG 455
            P  KAG           I  GDII    ++ VKN  +L  ++ +     +V LKI R  
Sbjct: 348 GPGYKAG-----------IKKGDIIIKFGDRLVKNTKKLRVIIADTPINQEVKLKILRDA 389

BLAST of Lcy09g003880 vs. ExPASy TrEMBL
Match: A0A6J1BXM7 (protease Do-like 8, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006537 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.7e-242
Identity = 438/461 (95.01%), Postives = 447/461 (96.96%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           MQVLACNAPH RTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPST N  TAQSISIQR
Sbjct: 1   MQVLACNAPHQRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTPNTGTAQSISIQR 60

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
           SA DDSGDLGSVLS KFL TTRRTLFASLF+S W+YPSSVFTAQALGDPSVTIEEVTPT+
Sbjct: 61  SARDDSGDLGSVLSRKFLSTTRRTLFASLFMSLWSYPSSVFTAQALGDPSVTIEEVTPTV 120

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
            PSG+LFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 121 FPSGTLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVL+VDA +DL
Sbjct: 181 VTNYHVIASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLQVDAPNDL 240

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVD APDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 361 INVDIAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           V+NKAELYKLLDEY AGD+VILKIKRGGQ LELPLILEESQ
Sbjct: 421 VRNKAELYKLLDEYNAGDEVILKIKRGGQTLELPLILEESQ 461

BLAST of Lcy09g003880 vs. ExPASy TrEMBL
Match: A0A5D3CVJ7 (Protease Do-like 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00330 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.5e-240
Identity = 435/461 (94.36%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTSTH LGRRELCFDGVSSVCS SQPSTSNPST++SI IQR
Sbjct: 10  MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 69

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV+S KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 70  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 129

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 130 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 189

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 190 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 249

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 250 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 309

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 310 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 369

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 370 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 429

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKLLDEY AGDK+ILKIKRGGQ LELPLILEESQ
Sbjct: 430 VKNKAELYKLLDEYNAGDKIILKIKRGGQTLELPLILEESQ 470

BLAST of Lcy09g003880 vs. ExPASy TrEMBL
Match: A0A1S3C9Y8 (protease Do-like 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498478 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.5e-240
Identity = 435/461 (94.36%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTSTH LGRRELCFDGVSSVCS SQPSTSNPST++SI IQR
Sbjct: 1   MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 60

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV+S KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 61  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 120

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 121 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 181 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 240

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKLLDEY AGDK+ILKIKRGGQ LELPLILEESQ
Sbjct: 421 VKNKAELYKLLDEYNAGDKIILKIKRGGQTLELPLILEESQ 461

BLAST of Lcy09g003880 vs. ExPASy TrEMBL
Match: A0A5A7T7R2 (Protease Do-like 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G003250 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.7e-239
Identity = 434/461 (94.14%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTSTH LGRRELCFDGVSSVCS SQPSTSNPST++SI IQR
Sbjct: 10  MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 69

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV+S KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 70  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 129

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 130 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 189

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 190 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 249

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 250 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 309

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 310 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 369

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 370 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 429

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKLLD+Y AGDK+ILKIKRGGQ LELPLILEESQ
Sbjct: 430 VKNKAELYKLLDDYNAGDKIILKIKRGGQTLELPLILEESQ 470

BLAST of Lcy09g003880 vs. ExPASy TrEMBL
Match: A0A6J1GY04 (protease Do-like 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111457871 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.2e-231
Identity = 425/461 (92.19%), Postives = 436/461 (94.58%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           MQVLACN P              HFLGRRELCFDGVSSV   SQPSTSNP+TAQSISIQR
Sbjct: 1   MQVLACNTP--------------HFLGRRELCFDGVSSV---SQPSTSNPTTAQSISIQR 60

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
           SAGD+SGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI
Sbjct: 61  SAGDESGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
            PSG+LFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 121 FPSGTLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS++L
Sbjct: 181 VTNYHVIASALARNPSPGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNEL 240

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPS TVVKIVPQLIQFGRV+RAG
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSLTVVKIVPQLIQFGRVIRAG 360

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALIL VPANSPAAKAGLLPTTRGFAGNIVLGDI+ AIDNKP
Sbjct: 361 INVDFAPDLIANQLNVRDGALILLVPANSPAAKAGLLPTTRGFAGNIVLGDIVTAIDNKP 420

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           V+NKAELYKLLDEY AGDKVILKI+RGGQ+LELPLILEESQ
Sbjct: 421 VRNKAELYKLLDEYNAGDKVILKIERGGQSLELPLILEESQ 444

BLAST of Lcy09g003880 vs. NCBI nr
Match: XP_022134229.1 (protease Do-like 8, chloroplastic [Momordica charantia] >XP_022134230.1 protease Do-like 8, chloroplastic [Momordica charantia])

HSP 1 Score: 846.7 bits (2186), Expect = 9.7e-242
Identity = 438/461 (95.01%), Postives = 447/461 (96.96%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           MQVLACNAPH RTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPST N  TAQSISIQR
Sbjct: 1   MQVLACNAPHQRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTPNTGTAQSISIQR 60

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
           SA DDSGDLGSVLS KFL TTRRTLFASLF+S W+YPSSVFTAQALGDPSVTIEEVTPT+
Sbjct: 61  SARDDSGDLGSVLSRKFLSTTRRTLFASLFMSLWSYPSSVFTAQALGDPSVTIEEVTPTV 120

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
            PSG+LFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 121 FPSGTLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVL+VDA +DL
Sbjct: 181 VTNYHVIASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLQVDAPNDL 240

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVD APDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 361 INVDIAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           V+NKAELYKLLDEY AGD+VILKIKRGGQ LELPLILEESQ
Sbjct: 421 VRNKAELYKLLDEYNAGDEVILKIKRGGQTLELPLILEESQ 461

BLAST of Lcy09g003880 vs. NCBI nr
Match: XP_008459309.1 (PREDICTED: protease Do-like 8, chloroplastic [Cucumis melo])

HSP 1 Score: 839.3 bits (2167), Expect = 1.5e-239
Identity = 435/461 (94.36%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTSTH LGRRELCFDGVSSVCS SQPSTSNPST++SI IQR
Sbjct: 1   MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 60

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV+S KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 61  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 120

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 121 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 181 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 240

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKLLDEY AGDK+ILKIKRGGQ LELPLILEESQ
Sbjct: 421 VKNKAELYKLLDEYNAGDKIILKIKRGGQTLELPLILEESQ 461

BLAST of Lcy09g003880 vs. NCBI nr
Match: TYK15258.1 (protease Do-like 8 [Cucumis melo var. makuwa])

HSP 1 Score: 839.3 bits (2167), Expect = 1.5e-239
Identity = 435/461 (94.36%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTSTH LGRRELCFDGVSSVCS SQPSTSNPST++SI IQR
Sbjct: 10  MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 69

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV+S KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 70  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 129

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 130 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 189

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 190 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 249

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 250 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 309

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 310 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 369

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 370 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 429

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKLLDEY AGDK+ILKIKRGGQ LELPLILEESQ
Sbjct: 430 VKNKAELYKLLDEYNAGDKIILKIKRGGQTLELPLILEESQ 470

BLAST of Lcy09g003880 vs. NCBI nr
Match: KAA0039504.1 (protease Do-like 8 [Cucumis melo var. makuwa])

HSP 1 Score: 838.2 bits (2164), Expect = 3.5e-239
Identity = 434/461 (94.14%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTSTH LGRRELCFDGVSSVCS SQPSTSNPST++SI IQR
Sbjct: 10  MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 69

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV+S KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 70  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 129

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 130 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 189

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 190 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 249

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 250 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 309

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 310 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 369

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 370 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 429

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKLLD+Y AGDK+ILKIKRGGQ LELPLILEESQ
Sbjct: 430 VKNKAELYKLLDDYNAGDKIILKIKRGGQTLELPLILEESQ 470

BLAST of Lcy09g003880 vs. NCBI nr
Match: XP_004148708.1 (protease Do-like 8, chloroplastic [Cucumis sativus] >KAE8648918.1 hypothetical protein Csa_008511 [Cucumis sativus])

HSP 1 Score: 828.6 bits (2139), Expect = 2.7e-236
Identity = 429/461 (93.06%), Postives = 440/461 (95.44%), Query Frame = 0

Query: 1   MQVLACNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSPSQPSTSNPSTAQSISIQR 60
           M VLACNAP LR M KSGVSTS   LGRRELCFDGVSSVCS SQPSTS+PST++SI IQR
Sbjct: 1   MHVLACNAPQLRMMAKSGVSTSAQMLGRRELCFDGVSSVCSSSQPSTSSPSTSESIFIQR 60

Query: 61  SAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSVTIEEVTPTI 120
            A DDSGDLGSV++ KFLFTTRRTLF SL LS W YPSSVFTAQALGDPSVTI+EVTPTI
Sbjct: 61  RADDDSGDLGSVVNRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 120

Query: 121 SPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180
           SPSGSLFPTEERI QLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI
Sbjct: 121 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDL 240
           VTNYHV+ASALARNPS+GQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDAS+DL
Sbjct: 181 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 240

Query: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAA 300
           LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQTDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360
           INPGNSGGPLLDSKGNLIGINTAIFTQTG SAGVGFAIPSSTVVKIVPQLIQFGRVVRAG
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGISAGVGFAIPSSTVVKIVPQLIQFGRVVRAG 360

Query: 361 INVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420
           INVDFAPDLI NQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP
Sbjct: 361 INVDFAPDLITNQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKP 420

Query: 421 VKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEESQ 462
           VKNKAELYKL+DEY AGDKVILKIKRGGQ+LELPLILEESQ
Sbjct: 421 VKNKAELYKLMDEYNAGDKVILKIKRGGQSLELPLILEESQ 461

BLAST of Lcy09g003880 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 605.9 bits (1561), Expect = 2.7e-173
Identity = 327/463 (70.63%), Postives = 373/463 (80.56%), Query Frame = 0

Query: 1   MQVLA--CNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSP-SQPSTSNPSTAQSIS 60
           MQV+A  C+ P+              F+GRR+L    +SSVCS  SQ    +     S+ 
Sbjct: 1   MQVIASFCSKPN-----------ENEFVGRRQL----LSSVCSKISQGDVVSHPPVSSVK 60

Query: 61  IQRSAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSV-TIEEV 120
           + +   D   +L  +       TTRR L  SLF++    PS   +A ALGDPSV T+E+V
Sbjct: 61  VTQ---DWKSNLHELAVKSVPSTTRRILLTSLFMNLCFNPSRYLSALALGDPSVATVEDV 120

Query: 121 TPTISPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDD 180
           +PT+ P+G LFPTE RIVQLFEKNTYSVVNIFDVTLRPQL +TG+VEIPEGNGSGVVWD 
Sbjct: 121 SPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDG 180

Query: 181 QGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDA 240
           QG+IVTNYHV+ +AL+RNPS G VV RVNILASDG+QKNFEGKL+GADR KDLAVLKVDA
Sbjct: 181 QGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDA 240

Query: 241 SDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQ 300
            + LL+PIKVGQS+SLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQ
Sbjct: 241 PETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQ 300

Query: 301 TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRV 360
           TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTV+KIVPQLIQF +V
Sbjct: 301 TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKV 360

Query: 361 VRAGINVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAI 420
           +RAGIN++ APD +ANQLNVR+GAL+LQVP  S A KAGL PT+RGFAGNIVLGDII A+
Sbjct: 361 LRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAV 420

Query: 421 DNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEE 460
           D+KPVKNKAEL K+LDEY  GDKV LKIKRG ++LEL + LEE
Sbjct: 421 DDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEE 445

BLAST of Lcy09g003880 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 570.9 bits (1470), Expect = 9.6e-163
Identity = 313/463 (67.60%), Postives = 359/463 (77.54%), Query Frame = 0

Query: 1   MQVLA--CNAPHLRTMVKSGVSTSTHFLGRRELCFDGVSSVCSP-SQPSTSNPSTAQSIS 60
           MQV+A  C+ P+              F+GRR+L    +SSVCS  SQ    +     S+ 
Sbjct: 1   MQVIASFCSKPN-----------ENEFVGRRQL----LSSVCSKISQGDVVSHPPVSSVK 60

Query: 61  IQRSAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTAQALGDPSV-TIEEV 120
           + +   D   +L  +       TTRR L  SLF++    PS   +A ALGDPSV T+E+V
Sbjct: 61  VTQ---DWKSNLHELAVKSVPSTTRRILLTSLFMNLCFNPSRYLSALALGDPSVATVEDV 120

Query: 121 TPTISPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDD 180
           +PT+ P+G LFPTE RIVQLFEKNTYSVVNIFDVTLRPQL +TG+VEIPEGNGSGVVWD 
Sbjct: 121 SPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDG 180

Query: 181 QGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDA 240
           QG+IVTNYHV+ +AL+RNPS G VV RVNILASDG+QKNFEGKL+GADR KDLAVLKVDA
Sbjct: 181 QGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDA 240

Query: 241 SDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGATIGGGIQ 300
            + LL+PIKVGQS+SLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG TIGGGIQ
Sbjct: 241 PETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQ 300

Query: 301 TDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRV 360
           TDAAINPGNSGGPLLDSKGNLIGINTAIFTQ              TV+KIVPQLIQF +V
Sbjct: 301 TDAAINPGNSGGPLLDSKGNLIGINTAIFTQ--------------TVLKIVPQLIQFSKV 360

Query: 361 VRAGINVDFAPDLIANQLNVRDGALILQVPANSPAAKAGLLPTTRGFAGNIVLGDIIAAI 420
           +RAGIN++ APD +ANQLNVR+GAL+LQVP  S A KAGL PT+RGFAGNIVLGDII A+
Sbjct: 361 LRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAV 420

Query: 421 DNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPLILEE 460
           D+KPVKNKAEL K+LDEY  GDKV LKIKRG ++LEL + LEE
Sbjct: 421 DDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEE 431

BLAST of Lcy09g003880 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 301.2 bits (770), Expect = 1.4e-81
Identity = 170/363 (46.83%), Postives = 234/363 (64.46%), Query Frame = 0

Query: 98  SSVFTAQALGDPSVTIEEVTPTISPSGSLFPTEE-RIVQLFEKNTYSVVNIFDVTLRPQL 157
           +SV  + +L   S  +E  +  +  +     T+E   V+LF++NT SVV I ++ +R   
Sbjct: 85  TSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVRLFQENTPSVVYITNLAVRQDA 144

Query: 158 NVTGMVEIPEGNGSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARVNILASDGIQKNF 217
               ++E+P+G+GSG VWD QGHIVTNYHV+  A           + + +  +D  Q  F
Sbjct: 145 FTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGA-----------SDLRVTLAD--QTTF 204

Query: 218 EGKLIGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVI 277
           + K++G D+ KD+AVL++DA  + LRPI VG S+ L VGQ+  AIGNPFG DHTLT GVI
Sbjct: 205 DAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTLTTGVI 264

Query: 278 SGLNRDIFS-QTGATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVG 337
           SGL R+I S  TG  I   IQTDAAINPGNSGGPLLDS G LIGINTAI++ +G S+GVG
Sbjct: 265 SGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVG 324

Query: 338 FAIPSSTVVKIVPQLIQFGRVVRAGINVDFAPDLIANQLNVRDGALILQVPANSPAAKAG 397
           F+IP  TV  IV QL++FG+V R  + + FAPD    QL V  G L+L  P + PA KAG
Sbjct: 325 FSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGV-SGVLVLDAPPSGPAGKAG 384

Query: 398 LLPTTRGFAGNIVLGDIIAAIDNKPVKNKAELYKLLDEYEAGDKVILKIKRGGQNLELPL 457
           L  T R   G +VLGDII +++   V N ++LY++LD+ + GD+V +++ RG    ++ +
Sbjct: 385 LQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEVLRGDHKEKISV 433

Query: 458 ILE 459
            LE
Sbjct: 445 TLE 433

BLAST of Lcy09g003880 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 199.5 bits (506), Expect = 5.8e-51
Identity = 122/276 (44.20%), Postives = 164/276 (59.42%), Query Frame = 0

Query: 89  LFLSSWAYPSSVFTAQALGDPSVTIEEVTPTISPSGSLFPTEERIVQLFEKNTYSVVNIF 148
           +F SS A  SS+  +     P  +   +         L   EER V LF+K + SVV I 
Sbjct: 47  IFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSVVYIE 106

Query: 149 DVTLRPQLNVTGMVEIP----EGNGSGVVWDDQGHIVTNYHVVASALARNPSSGQVVARV 208
            + L    +   + +      EG GSG VWD  GHIVTNYHV+A         G    +V
Sbjct: 107 AIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAK--LATDQFGLQRCKV 166

Query: 209 NILASDGIQKNFEGKLIGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNP 268
           +++ + G + + EGK++G D   DLAVLK++     L P+ +G S+ L+VGQ C AIGNP
Sbjct: 167 SLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNP 226

Query: 269 FGFDHTLTVGVISGLNRDIFSQTGATIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAI 328
           +G+++TLT+GV+SGL R+I S  G +I   IQTDA IN GNSGGPLLDS G+ IG+NTA 
Sbjct: 227 YGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSGGPLLDSYGHTIGVNTAT 286

Query: 329 FTQ--TGTSAGVGFAIPSSTVVKIVPQLIQFGRVVR 359
           FT+  +G S+GV FAIP  TVV+ VP LI +G   R
Sbjct: 287 FTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYR 320

BLAST of Lcy09g003880 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 119.8 bits (299), Expect = 5.8e-27
Identity = 120/395 (30.38%), Postives = 185/395 (46.84%), Query Frame = 0

Query: 46  STSNPS--TAQSISIQRSAGDDSGDLGSVLSSKFLFTTRRTLFASLFLSSWAYPSSVFTA 105
           +++NP   T  S++I  S  +    L   +S   +    ++LF +   SS   P S   A
Sbjct: 33  ASTNPDARTRVSLAIPESVRESLSLLPWQISPGLIHRPEQSLFGNFVFSSRVSPKS--EA 92

Query: 106 QALGDPSVTIEEVTPTISPSGSLFPTEERIVQLFEKNTYSVVNIFDVTLRPQLNVTGMVE 165
               +  V++E    +  PS   +   + I     +   +VVN+      PQ    G   
Sbjct: 93  PINDEKGVSVEASDSSSKPSNG-YLGRDTIANAAARIGPAVVNL----SVPQ----GFHG 152

Query: 166 IPEGN--GSGVVWDDQGHIVTNYHVVAS-ALARNPSSGQVVARVNILASDGIQKNFEGKL 225
           I  G   GSG + D  G I+T  HVV      R+ S G    RV++   DG  + FEG +
Sbjct: 153 ISMGKSIGSGTIIDADGTILTCAHVVVDFQNIRHSSKG----RVDVTLQDG--RTFEGVV 212

Query: 226 IGADRTKDLAVLKVDASDDLLRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLN 285
           + AD   D+A++K+  S   L   K+G SS L+ G   +A+G P    +T+T G++S ++
Sbjct: 213 VNADLQSDIALVKI-KSKTPLPTAKLGFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVD 272

Query: 286 RDIFSQTGATIGGG----IQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGF 345
           R     +   +GG     +QTD +IN GNSGGPL++  G +IG+N     +   + G+GF
Sbjct: 273 R---KSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVN---IMKVLAADGLGF 332

Query: 346 AIPSSTVVKIVPQLIQFGRVVRAGINVDFAP--DLIANQLNVRD--------GALILQVP 405
           ++P  +V KI+    + GRV+R  I +      +LI  QL  RD        G L+  V 
Sbjct: 333 SVPIDSVSKIIEHFKKSGRVIRPWIGLKMVELNNLIVAQLKERDPMFPDVERGVLVPTVI 392

Query: 406 ANSPAAKAGLLPTTRGFAGNIVLGDIIAAIDNKPV 422
             SPA +AG  P           GD++   D KPV
Sbjct: 393 PGSPADRAGFKP-----------GDVVVRFDGKPV 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LU103.8e-17270.63Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ... [more]
O226092.0e-8046.83Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ... [more]
Q9SEL78.2e-5044.20Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ... [more]
F6AA629.7e-4339.79Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fulva (strain ... [more]
O059424.8e-4238.76Probable periplasmic serine endoprotease DegP-like OS=Rickettsia prowazekii (str... [more]
Match NameE-valueIdentityDescription
A0A6J1BXM74.7e-24295.01protease Do-like 8, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006537... [more]
A0A5D3CVJ77.5e-24094.36Protease Do-like 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G0... [more]
A0A1S3C9Y87.5e-24094.36protease Do-like 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498478 PE=3 S... [more]
A0A5A7T7R21.7e-23994.14Protease Do-like 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00... [more]
A0A6J1GY042.2e-23192.19protease Do-like 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111457871 ... [more]
Match NameE-valueIdentityDescription
XP_022134229.19.7e-24295.01protease Do-like 8, chloroplastic [Momordica charantia] >XP_022134230.1 protease... [more]
XP_008459309.11.5e-23994.36PREDICTED: protease Do-like 8, chloroplastic [Cucumis melo][more]
TYK15258.11.5e-23994.36protease Do-like 8 [Cucumis melo var. makuwa][more]
KAA0039504.13.5e-23994.14protease Do-like 8 [Cucumis melo var. makuwa][more]
XP_004148708.12.7e-23693.06protease Do-like 8, chloroplastic [Cucumis sativus] >KAE8648918.1 hypothetical p... [more]
Match NameE-valueIdentityDescription
AT5G39830.12.7e-17370.63Trypsin family protein with PDZ domain [more]
AT5G39830.29.6e-16367.60Trypsin family protein with PDZ domain [more]
AT3G27925.11.4e-8146.83DegP protease 1 [more]
AT4G18370.15.8e-5144.20DEGP protease 5 [more]
AT5G27660.15.8e-2730.38Trypsin family protein with PDZ domain [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001940Peptidase S1CPRINTSPR00834PROTEASES2Ccoord: 178..190
score: 56.85
coord: 313..330
score: 45.12
coord: 254..278
score: 51.12
coord: 291..308
score: 69.24
coord: 406..418
score: 31.71
IPR001478PDZ domainSMARTSM00228pdz_newcoord: 332..448
e-value: 2.5E-4
score: 30.4
IPR001478PDZ domainPFAMPF13180PDZ_2coord: 376..455
e-value: 9.6E-13
score: 48.4
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 124..244
e-value: 2.6E-32
score: 112.9
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 245..358
e-value: 7.7E-41
score: 140.9
NoneNo IPR availablePFAMPF13365Trypsin_2coord: 169..320
e-value: 1.3E-29
score: 104.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..66
NoneNo IPR availablePANTHERPTHR43343:SF3PROTEASE DO-LIKE 8, CHLOROPLASTICcoord: 63..460
NoneNo IPR availablePANTHERPTHR43343PEPTIDASE S12coord: 63..460
NoneNo IPR availableCDDcd00987PDZ_serine_proteasecoord: 360..455
e-value: 3.76836E-19
score: 79.9911
IPR036034PDZ superfamilyGENE3D2.30.42.10coord: 360..461
e-value: 1.2E-30
score: 107.3
IPR036034PDZ superfamilySUPERFAMILY50156PDZ domain-likecoord: 329..459
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 133..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g003880.1Lcy09g003880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010206 photosystem II repair
biological_process GO:0006508 proteolysis
cellular_component GO:0009543 chloroplast thylakoid lumen
molecular_function GO:0005515 protein binding
molecular_function GO:0004252 serine-type endopeptidase activity