Lcy09g002890 (gene) Sponge gourd (P93075) v1

BLAST of Lcy09g002890 vs. ExPASy Swiss-Prot
Match: O67409 (Tryptophan synthase beta chain 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 6.9e-148
Identity = 255/425 (60.00%), Postives = 312/425 (73.41%), Query Frame = 0

Query: 50  EIPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVV 109
           EIP++W N++  LP    P L P T +P+KP+ L  +FP  L+ Q+ +   +I+IP+EV+
Sbjct: 10  EIPKKWLNILPLLPEPLEPPLDPETMEPVKPEKLLAIFPEPLVEQEVSDKEWIDIPEEVL 69

Query: 110 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNV 169
           DIY LWRPTPL RAK LE+ L TPA+I+YK E VSP GSHK N+AV Q +Y+   GVK +
Sbjct: 70  DIYSLWRPTPLHRAKNLEEFLGTPAKIFYKNESVSPPGSHKPNTAVAQAYYNKISGVKRL 129

Query: 170 VTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNE 229
            TETGAGQWGSAL+FA   F L C+V+ VR SY+QKPYRR++M+TW  +V PSPS  TN 
Sbjct: 130 TTETGAGQWGSALSFATQFFDLQCRVYMVRVSYNQKPYRRILMETWKGEVIPSPSPYTNA 189

Query: 230 GRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEA 289
           GRK  +  P  PGSL IAISEA+E AA   DTKY +GSVLNHVLLHQTVIG E  KQME 
Sbjct: 190 GRKYYEENPEHPGSLGIAISEAIEEAASREDTKYSLGSVLNHVLLHQTVIGLEAKKQMEE 249

Query: 290 LGESPDVIIGCTGGGSNFAGLSFPFLREKFAG---KISPVIRAVEPAACPSLTKGVYAYD 349
            G  PDVIIG  GGGSNFAGLSFPFL +   G   K    + AVEP ACP+LTKG Y YD
Sbjct: 250 AGYYPDVIIGAVGGGSNFAGLSFPFLADVLRGDKRKEDLKVLAVEPEACPTLTKGEYKYD 309

Query: 350 YADTARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQ 409
           + D+   TPL+KM+TLGH+F+P PIHA GLRYHG APL+  +YNLG ++A++  QTE F+
Sbjct: 310 FGDSVGLTPLIKMYTLGHDFVPSPIHAGGLRYHGDAPLVCKLYNLGYIDAVAYKQTEVFE 369

Query: 410 GAVQFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQ 469
            AV FAR EG++PAPE+ HAI A I EAL+C+ETGE KVIL  +SGHG+FDL AYDKYL 
Sbjct: 370 AAVTFARTEGIVPAPESAHAIKAAIDEALKCKETGEEKVILFNLSGHGYFDLSAYDKYLH 429

Query: 470 GGMID 472
           G + D
Sbjct: 430 GELTD 434

BLAST of Lcy09g002890 vs. ExPASy Swiss-Prot
Match: Q8TL44 (Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.4e-147
Identity = 242/421 (57.48%), Postives = 319/421 (75.77%), Query Frame = 0

Query: 50  EIPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVV 109
           E+P++WYN++ADLP    P L P T+QPI P  L P+FP  LI Q+ ++DRYI+IP+EV+
Sbjct: 22  EMPKKWYNVLADLPSPIDPPLDPRTWQPISPDALEPIFPKALIMQEMSSDRYIDIPEEVL 81

Query: 110 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNV 169
           D+Y LWRP+PL RA +LEK+L +PA+IYYKYEGVSPAGSHK N+++ Q +Y+++EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKVLKSPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 170 VTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNE 229
            TETGAGQWGSAL+ AC+ F L C+V+ VR+S+ QKPYR+ ++  WG  V PSPS  T  
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSFYQKPYRKSLITLWGGNVVPSPSPDTEF 201

Query: 230 GRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEA 289
           GRK+LQ +P +PGSL IAISEA+E A  + +TKY +GSVLNHV+LHQTVIG EC +Q+  
Sbjct: 202 GRKILQEQPDTPGSLGIAISEAVEDAIAHENTKYSLGSVLNHVVLHQTVIGAECKQQLAQ 261

Query: 290 LGESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYAD 349
           + E PDV+IGC GGGSN  G+   F++++  GK S  + AVEP+ACPSLTKG Y YD+ D
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHSARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 350 TARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAV 409
           TA  TPL+KM+TLGH  +P  IHA GLRYHG +P+IS + + GL+EA+S  Q E F  AV
Sbjct: 322 TAEMTPLLKMYTLGHKHVPPAIHAGGLRYHGDSPIISKLCSEGLMEAVSYDQQEVFDAAV 381

Query: 410 QFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGM 469
           QFAR EG++PAPE++HAI   I EAL  ++TGE K IL  +SGHGHFD+ +YDKY  G +
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALAAKQTGEEKTILFNLSGHGHFDMSSYDKYFSGEL 441

Query: 470 I 471
           +
Sbjct: 442 M 442

BLAST of Lcy09g002890 vs. ExPASy Swiss-Prot
Match: Q8Q001 (Tryptophan synthase beta chain 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.9e-147
Identity = 244/418 (58.37%), Postives = 316/418 (75.60%), Query Frame = 0

Query: 50  EIPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVV 109
           E+P++WYN+++DLP    P L P T+QPI P+ L P+F  ELI Q+ ++DRYI+IP E++
Sbjct: 22  EMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEIL 81

Query: 110 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNV 169
           D+Y LWRP+PL RA +LEK L TPA+IYYKYEGVSPAGSHK N+++ Q +Y+++EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 170 VTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNE 229
            TETGAGQWGSAL+ AC+ F L C+V+ VR+SY QKPYR+ +M  WG  V PSPS  T  
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSYYQKPYRKSLMTLWGGNVVPSPSPDTEF 201

Query: 230 GRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEA 289
           GRK+L+ +P +PGSL IAISEA+E A  + +TKY +GSVLNHV+LHQTVIG EC KQ+E 
Sbjct: 202 GRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTVIGAECKKQLEQ 261

Query: 290 LGESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYAD 349
           + E PDV+IGC GGGSN  G+   F++++  GK +  + AVEP+ACPSLTKG Y YD+ D
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 350 TARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAV 409
           TA  TPL+KM+TLGH  IP  IHA GLRYHG +P+IS +   GL+EA+S  Q E F  AV
Sbjct: 322 TAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAV 381

Query: 410 QFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQG 468
           QFAR EG++PAPE++HAI   I EAL  ++ GE KVIL  +SGHGHFD+ +YDKY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDMASYDKYFSG 439

BLAST of Lcy09g002890 vs. ExPASy Swiss-Prot
Match: O29028 (Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 247/422 (58.53%), Postives = 313/422 (74.17%), Query Frame = 0

Query: 50  EIPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVV 109
           E+P++WYN++ DLP   PP LHP T +P+KP+DL P+FP  LI Q+ + +R+I IP++V 
Sbjct: 15  EMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIPEDVR 74

Query: 110 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNV 169
           +IY +WRPTPL+RA+RLEK L TPARIY+KYEG SP GSHK N+AV Q +Y+ +EGV+ +
Sbjct: 75  EIYRIWRPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEGVERL 134

Query: 170 VTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNE 229
            TETGAGQWGSAL FA  LF + C V+ V+ S+ QKPYRR+MM+TWG +V PSPSD T  
Sbjct: 135 TTETGAGQWGSALCFATKLFEMACTVYMVKVSFMQKPYRRVMMETWGGEVIPSPSDRTEV 194

Query: 230 GRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEA 289
           GRK+L   P +PGSL IAISEA+E AA N +TKY +GSVLNHVLLHQTVIG E   Q+E 
Sbjct: 195 GRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLHQTVIGLETKAQLEK 254

Query: 290 LGESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYAD 349
           + E PDV+IGC GGGSNFAGL++PF+ +   G +   I AVEPAACP+LT G Y YD+ D
Sbjct: 255 VDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLE--IIAVEPAACPTLTAGEYKYDFGD 314

Query: 350 TARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAV 409
            A  TPL+KM+TLGH+FIP PIHA GLRYHG AP +  +   G+++A ++ Q   F+  +
Sbjct: 315 VAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGL 374

Query: 410 QFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGM 469
            FAR EG+IPAPET HA+ A I EA++ RE  E KVI+   SGHG  DL AYD YL G +
Sbjct: 375 LFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLDLQAYDDYLAGRL 434

Query: 470 ID 472
            D
Sbjct: 435 AD 434

BLAST of Lcy09g002890 vs. ExPASy Swiss-Prot
Match: Q9WZ09 (Tryptophan synthase beta chain 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.0e-138
Identity = 243/417 (58.27%), Postives = 307/417 (73.62%), Query Frame = 0

Query: 45  NLKAIEIPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINI 104
           NLK  EIP+ WYN++ADLP K  P L P T QPI P+ LS +FP  LI Q+ + +R+I I
Sbjct: 6   NLKPEEIPKHWYNVLADLPFKLDPPLDPETKQPISPEKLSVIFPMSLIEQEVSEERFIEI 65

Query: 105 PDEVVDIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQE 164
           P+ V+  Y ++RPTPLIRA  LE+ L TPARIYYKYEGVSP GSHK N+A+ Q +Y+  E
Sbjct: 66  PEPVLKEYAVYRPTPLIRATFLEEYLQTPARIYYKYEGVSPTGSHKPNTAIAQAYYNKIE 125

Query: 165 GVKNVVTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPS 224
           GVK +VTETGAGQWGSAL++A + FGL  +V+ V+ SY QKP R+ MM  +G KV PSPS
Sbjct: 126 GVKRLVTETGAGQWGSALSYAGAKFGLEVKVFMVKVSYQQKPMRKYMMNLFGGKVTPSPS 185

Query: 225 DLTNEGRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECI 284
           + TN GRK+L  +P +PGSL IAISEA+EVA  + +TKY +GSVLNHVLLHQTVIG E  
Sbjct: 186 EETNFGRKILSEDPDNPGSLGIAISEALEVAVSDPNTKYSLGSVLNHVLLHQTVIGLEIK 245

Query: 285 KQMEALGESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYA 344
           KQ+E +GE PD+++GC GGGSNF G   PF+ +K +G+    + A EPAACPSLTKG Y 
Sbjct: 246 KQLELIGEKPDILLGCHGGGSNFGGTILPFVPDKLSGRDIRFV-ACEPAACPSLTKGNYD 305

Query: 345 YDYADTARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTEC 404
           YD+ DTA  TPL+KM+TLG +FIP  IHA GLRYHG AP+I+ +   GL+EA +  Q E 
Sbjct: 306 YDFGDTAGLTPLLKMYTLGKDFIPPKIHAGGLRYHGSAPIIARLVKEGLVEAQAFDQDET 365

Query: 405 FQGAVQFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAY 462
           F+ A  FA+ EG+IPAPE+ HAIA  IREA + +E G+ +VI+  +SGHG  DL AY
Sbjct: 366 FEAAKIFAKLEGIIPAPESAHAIAGAIREAKKAKEEGKERVIVFTLSGHGLLDLTAY 421

BLAST of Lcy09g002890 vs. ExPASy TrEMBL
Match: A0A6J1BXI7 (Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111006607 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.1e-252
Identity = 436/500 (87.20%), Postives = 463/500 (92.60%), Query Frame = 0

Query: 1   MAAHF---ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEI 60
           MA+HF   ASS S  ING+VGE+LP G +S+KRNP      NGYRT+AASN  NLK +E+
Sbjct: 1   MASHFLVAASSTSPAINGRVGEELP-GRLSLKRNPRNLRFSNGYRTRAASNR-NLKPVEV 60

Query: 61  PRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDI 120
           PR+WYNLVADLPVKPPP LHP TFQPIKPQDLSPLFP  LI Q+A+TDRYINIPDEVVDI
Sbjct: 61  PRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDI 120

Query: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVT 180
           YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+VQEGVKNVVT
Sbjct: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVT 180

Query: 181 ETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGR 240
           ETGAGQWGSALAFACS+FGLGC+VWQVRASYDQKPYRRLMMQTWG KVHPSPSDLT+ GR
Sbjct: 181 ETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGR 240

Query: 241 KLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALG 300
           KLLQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQMEALG
Sbjct: 241 KLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALG 300

Query: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTA 360
           ESPDVIIGCTGGGSNFAGLSFPFLREK AGKI+P++RAVEPAACPSLTKGVYAYD+ DTA
Sbjct: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTA 360

Query: 361 RTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQF 420
             TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEAISLPQTECFQGA+QF
Sbjct: 361 GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQF 420

Query: 421 ARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMID 480
           AR EGLI APE THAIAATIREALRCRETGESKVILTAM GHGHFDLPAY+KYLQGGM D
Sbjct: 421 ARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTD 480

Query: 481 LSFAEEKIQESLTSSIPQLA 492
           LSFAEEKIQESLT +IPQ+A
Sbjct: 481 LSFAEEKIQESLT-TIPQVA 497

BLAST of Lcy09g002890 vs. ExPASy TrEMBL
Match: A0A0A0KUJ4 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_5G643330 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.7e-246
Identity = 425/500 (85.00%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MAAHF---ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEI 60
           MAA F    S  SS IN  VGEQLP GC+S+KR P      NGYRTKAAS NPNLK++E+
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLP-GCLSLKRVPAHLRFSNGYRTKAAS-NPNLKSVEV 60

Query: 61  PRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDI 120
           PRQWYNLVADLPVKPPP LHP T++PIKP+DLS LFP ELI Q+A+T+RYINIPDEVVDI
Sbjct: 61  PRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDI 120

Query: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVT 180
           YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+ QEGVKNVVT
Sbjct: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVT 180

Query: 181 ETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGR 240
           ETGAGQWG +LAFACS+FG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPSD+T+ GR
Sbjct: 181 ETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGR 240

Query: 241 KLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALG 300
           K LQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQ+EALG
Sbjct: 241 KFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALG 300

Query: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTA 360
           E+PD+IIGCTGGGSNFAGLSFPFLREK AG I+PVIRAVEPAACPSLTKGVYAYDY DTA
Sbjct: 301 ETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTA 360

Query: 361 RTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQF 420
             TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEAISLPQTECFQGA+QF
Sbjct: 361 GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQF 420

Query: 421 ARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMID 480
           AR EGLIPAPE THAIAATIREALRCRETGESKVILTAM GHGHFDLPAY+KYLQGG+ D
Sbjct: 421 ARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISD 480

Query: 481 LSFAEEKIQESLTSSIPQLA 492
           LSFA EKIQESLT +IPQ+A
Sbjct: 481 LSFANEKIQESLT-TIPQVA 496

BLAST of Lcy09g002890 vs. ExPASy TrEMBL
Match: A0A1S3CA62 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498574 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.8e-245
Identity = 423/500 (84.60%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MAAHF---ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEI 60
           MAA F    S  SS IN  VGEQLP GC+S+KR P      NGYRTK+AS NPNLKA+EI
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLP-GCLSLKRVPTHLRFSNGYRTKSAS-NPNLKAVEI 60

Query: 61  PRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDI 120
           PRQWYNLVADLPVKPPP LHP T++PIKP+DLS LFP ELI Q+A+T+RYINIPDEVVDI
Sbjct: 61  PRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDI 120

Query: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVT 180
           YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+ QEGVKNVVT
Sbjct: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVT 180

Query: 181 ETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGR 240
           ETGAGQWG +LAFACS+FG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPSD+T+ GR
Sbjct: 181 ETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDVTDAGR 240

Query: 241 KLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALG 300
           K LQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQ+EALG
Sbjct: 241 KFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALG 300

Query: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTA 360
           ESPD+IIGCTGGGSNFAGLSFPFLREK AG I+PVIRAVEPAACPSLTKGVYAYDY DTA
Sbjct: 301 ESPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTA 360

Query: 361 RTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQF 420
             TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGA++F
Sbjct: 361 GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIKF 420

Query: 421 ARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMID 480
           AR EGLIPAPE THAIAATIREALRCRETGESKVILTAM GHGHFDLPAY+KYLQGG+ D
Sbjct: 421 ARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITD 480

Query: 481 LSFAEEKIQESLTSSIPQLA 492
           LSFA EK++ESLT +IPQ+A
Sbjct: 481 LSFANEKMKESLT-TIPQVA 496

BLAST of Lcy09g002890 vs. ExPASy TrEMBL
Match: A0A6J1JY44 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111489338 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.2e-245
Identity = 420/491 (85.54%), Postives = 451/491 (91.85%), Query Frame = 0

Query: 6   ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEIPRQWYNLV 65
           ASS SS ING        GC+S++R P      NGYRTKAAS NPNLKA+E+PRQWYNLV
Sbjct: 3   ASSISSGING-------GGCLSIERIPRHLRFSNGYRTKAAS-NPNLKAVEVPRQWYNLV 62

Query: 66  ADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDIYGLWRPTP 125
           ADLPVKPPP LHP TF+PIKP+DLSPLFP ELI Q+A+ +RYI+IPDEVVDIYGLWRPTP
Sbjct: 63  ADLPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTP 122

Query: 126 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVTETGAGQWG 185
           LIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+VQEGVKNVVTETGAGQWG
Sbjct: 123 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWG 182

Query: 186 SALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGRKLLQVEPS 245
            +LAFACSLFG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPS++T+ GRK LQV+PS
Sbjct: 183 CSLAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPS 242

Query: 246 SPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALGESPDVIIG 305
           SPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQMEALGE+PDVIIG
Sbjct: 243 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIG 302

Query: 306 CTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTARTTPLMKM 365
           CTGGGSNFAGLSFPFLREK AGKISPVIRAVEPAACPSLTKGVYAYDY DTA  TPLMKM
Sbjct: 303 CTGGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKM 362

Query: 366 HTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQFARCEGLIP 425
           HTLGHNFIPDPIHA GLRYHGMAPLISHVY+LGLLEA+SLPQTECFQGA+QFAR EGLIP
Sbjct: 363 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYDLGLLEAVSLPQTECFQGAIQFARSEGLIP 422

Query: 426 APETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMIDLSFAEEKI 485
           APE THAIA TIREALRCRETGESKVILTAM GHGHFDL AY+KYLQGGM DLSFAEEKI
Sbjct: 423 APEPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKI 482

Query: 486 QESLTSSIPQL 491
           +ESLT +IPQL
Sbjct: 483 KESLT-TIPQL 484

BLAST of Lcy09g002890 vs. ExPASy TrEMBL
Match: A0A6J1FH74 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111445435 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.2e-243
Identity = 419/491 (85.34%), Postives = 449/491 (91.45%), Query Frame = 0

Query: 6   ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEIPRQWYNLV 65
           AS  SS ING        GC+S++R P      NGYRTKAAS NPNLKA+E+PRQWYNLV
Sbjct: 3   ASLISSGING-------GGCLSLERIPRHLRFSNGYRTKAAS-NPNLKAVEVPRQWYNLV 62

Query: 66  ADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDIYGLWRPTP 125
           ADLPVKPPP LHP TF+PIKP+DLSPLFP ELI Q+A+ +RYI+IPDEVVDIYGLWRPTP
Sbjct: 63  ADLPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTP 122

Query: 126 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVTETGAGQWG 185
           LIRAKRLEKLL TPARIYYKYEGVSPAGSHK NSAVPQVWY+VQEGVKNVVTETGAGQWG
Sbjct: 123 LIRAKRLEKLLGTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWG 182

Query: 186 SALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGRKLLQVEPS 245
            +LAFACSLFG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPS++T+ GRK LQV+PS
Sbjct: 183 CSLAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPS 242

Query: 246 SPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALGESPDVIIG 305
           SPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQMEALGE+PDVIIG
Sbjct: 243 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIG 302

Query: 306 CTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTARTTPLMKM 365
           CTGGGSNFAGLSFPFLREK AGKISPVIRAVEPAACPSLTKGVYAYDY DTA  TPLMKM
Sbjct: 303 CTGGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKM 362

Query: 366 HTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQFARCEGLIP 425
           HTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGA+QFAR EGLIP
Sbjct: 363 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIQFARSEGLIP 422

Query: 426 APETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMIDLSFAEEKI 485
           APE THAIA TIREALRCRETGESKVILTAM GHGHFDL AY+KYLQGGM DLSFAEEKI
Sbjct: 423 APEPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKI 482

Query: 486 QESLTSSIPQL 491
           +ESLT +IPQL
Sbjct: 483 KESLT-TIPQL 484

BLAST of Lcy09g002890 vs. NCBI nr
Match: XP_022134316.1 (uncharacterized protein LOC111006607 [Momordica charantia])

HSP 1 Score: 882.1 bits (2278), Expect = 2.2e-252
Identity = 436/500 (87.20%), Postives = 463/500 (92.60%), Query Frame = 0

Query: 1   MAAHF---ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEI 60
           MA+HF   ASS S  ING+VGE+LP G +S+KRNP      NGYRT+AASN  NLK +E+
Sbjct: 1   MASHFLVAASSTSPAINGRVGEELP-GRLSLKRNPRNLRFSNGYRTRAASNR-NLKPVEV 60

Query: 61  PRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDI 120
           PR+WYNLVADLPVKPPP LHP TFQPIKPQDLSPLFP  LI Q+A+TDRYINIPDEVVDI
Sbjct: 61  PRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDI 120

Query: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVT 180
           YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+VQEGVKNVVT
Sbjct: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVT 180

Query: 181 ETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGR 240
           ETGAGQWGSALAFACS+FGLGC+VWQVRASYDQKPYRRLMMQTWG KVHPSPSDLT+ GR
Sbjct: 181 ETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGR 240

Query: 241 KLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALG 300
           KLLQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQMEALG
Sbjct: 241 KLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALG 300

Query: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTA 360
           ESPDVIIGCTGGGSNFAGLSFPFLREK AGKI+P++RAVEPAACPSLTKGVYAYD+ DTA
Sbjct: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTA 360

Query: 361 RTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQF 420
             TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEAISLPQTECFQGA+QF
Sbjct: 361 GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQF 420

Query: 421 ARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMID 480
           AR EGLI APE THAIAATIREALRCRETGESKVILTAM GHGHFDLPAY+KYLQGGM D
Sbjct: 421 ARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTD 480

Query: 481 LSFAEEKIQESLTSSIPQLA 492
           LSFAEEKIQESLT +IPQ+A
Sbjct: 481 LSFAEEKIQESLT-TIPQVA 497

BLAST of Lcy09g002890 vs. NCBI nr
Match: XP_031741291.1 (uncharacterized protein LOC101219640 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 5.3e-246
Identity = 426/500 (85.20%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MAAHF---ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEI 60
           MAA F    S  SS IN  VGEQLP GC+S+KR P      NGYRTKAAS NPNLKA+E+
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLP-GCLSLKRVPAHLRFSNGYRTKAAS-NPNLKAVEV 60

Query: 61  PRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDI 120
           PRQWYNLVADLPVKPPP LHP T++PIKP+DLS LFP ELI Q+A+T+RYINIPDEVVDI
Sbjct: 61  PRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDI 120

Query: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVT 180
           YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+ QEGVKNVVT
Sbjct: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVT 180

Query: 181 ETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGR 240
           ETGAGQWG +LAFACS+FG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPSD+T+ GR
Sbjct: 181 ETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGR 240

Query: 241 KLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALG 300
           K LQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQ+EALG
Sbjct: 241 KFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALG 300

Query: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTA 360
           E+PD+IIGCTGGGSNFAGLSFPFLREK AG I+PVIRAVEPAACPSLTKGVYAYDY DTA
Sbjct: 301 ETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTA 360

Query: 361 RTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQF 420
             TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEAISLPQTECFQGA+QF
Sbjct: 361 GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQF 420

Query: 421 ARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMID 480
           AR EGLIPAPE THAIAATIREALRCRETGESKVILTAM GHGHFDLPAY+KYLQGG+ D
Sbjct: 421 ARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISD 480

Query: 481 LSFAEEKIQESLTSSIPQLA 492
           LSFA EKIQESLT +IPQ+A
Sbjct: 481 LSFANEKIQESLT-TIPQVA 496

BLAST of Lcy09g002890 vs. NCBI nr
Match: XP_008459440.1 (PREDICTED: tryptophan synthase beta chain 2-like [Cucumis melo])

HSP 1 Score: 856.7 bits (2212), Expect = 1.0e-244
Identity = 423/500 (84.60%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MAAHF---ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEI 60
           MAA F    S  SS IN  VGEQLP GC+S+KR P      NGYRTK+AS NPNLKA+EI
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLP-GCLSLKRVPTHLRFSNGYRTKSAS-NPNLKAVEI 60

Query: 61  PRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDI 120
           PRQWYNLVADLPVKPPP LHP T++PIKP+DLS LFP ELI Q+A+T+RYINIPDEVVDI
Sbjct: 61  PRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDI 120

Query: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVT 180
           YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+ QEGVKNVVT
Sbjct: 121 YGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVT 180

Query: 181 ETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGR 240
           ETGAGQWG +LAFACS+FG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPSD+T+ GR
Sbjct: 181 ETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDVTDAGR 240

Query: 241 KLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALG 300
           K LQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQ+EALG
Sbjct: 241 KFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALG 300

Query: 301 ESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTA 360
           ESPD+IIGCTGGGSNFAGLSFPFLREK AG I+PVIRAVEPAACPSLTKGVYAYDY DTA
Sbjct: 301 ESPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTA 360

Query: 361 RTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQF 420
             TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGA++F
Sbjct: 361 GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIKF 420

Query: 421 ARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMID 480
           AR EGLIPAPE THAIAATIREALRCRETGESKVILTAM GHGHFDLPAY+KYLQGG+ D
Sbjct: 421 ARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITD 480

Query: 481 LSFAEEKIQESLTSSIPQLA 492
           LSFA EK++ESLT +IPQ+A
Sbjct: 481 LSFANEKMKESLT-TIPQVA 496

BLAST of Lcy09g002890 vs. NCBI nr
Match: XP_022993275.1 (uncharacterized protein LOC111489338 [Cucurbita maxima])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 420/491 (85.54%), Postives = 451/491 (91.85%), Query Frame = 0

Query: 6   ASSFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIEIPRQWYNLV 65
           ASS SS ING        GC+S++R P      NGYRTKAAS NPNLKA+E+PRQWYNLV
Sbjct: 3   ASSISSGING-------GGCLSIERIPRHLRFSNGYRTKAAS-NPNLKAVEVPRQWYNLV 62

Query: 66  ADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVDIYGLWRPTP 125
           ADLPVKPPP LHP TF+PIKP+DLSPLFP ELI Q+A+ +RYI+IPDEVVDIYGLWRPTP
Sbjct: 63  ADLPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTP 122

Query: 126 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVTETGAGQWG 185
           LIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWY+VQEGVKNVVTETGAGQWG
Sbjct: 123 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWG 182

Query: 186 SALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGRKLLQVEPS 245
            +LAFACSLFG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPS++T+ GRK LQV+PS
Sbjct: 183 CSLAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPS 242

Query: 246 SPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEALGESPDVIIG 305
           SPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQMEALGE+PDVIIG
Sbjct: 243 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIG 302

Query: 306 CTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADTARTTPLMKM 365
           CTGGGSNFAGLSFPFLREK AGKISPVIRAVEPAACPSLTKGVYAYDY DTA  TPLMKM
Sbjct: 303 CTGGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKM 362

Query: 366 HTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQFARCEGLIP 425
           HTLGHNFIPDPIHA GLRYHGMAPLISHVY+LGLLEA+SLPQTECFQGA+QFAR EGLIP
Sbjct: 363 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYDLGLLEAVSLPQTECFQGAIQFARSEGLIP 422

Query: 426 APETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMIDLSFAEEKI 485
           APE THAIA TIREALRCRETGESKVILTAM GHGHFDL AY+KYLQGGM DLSFAEEKI
Sbjct: 423 APEPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKI 482

Query: 486 QESLTSSIPQL 491
           +ESLT +IPQL
Sbjct: 483 KESLT-TIPQL 484

BLAST of Lcy09g002890 vs. NCBI nr
Match: XP_038890851.1 (tryptophan synthase beta chain 2 [Benincasa hispida])

HSP 1 Score: 854.4 bits (2206), Expect = 5.0e-244
Identity = 423/500 (84.60%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MAAHFAS----SFSSCINGKVGEQLPAGCVSVKRNP------NGYRTKAASNNPNLKAIE 60
           MAA F +    + S   NG+VGEQL +G +S+KR P      NGYRTKAAS NPNLKA+E
Sbjct: 1   MAATFLAVGSLTSSGINNGRVGEQL-SGYLSLKRVPRHLRFSNGYRTKAAS-NPNLKAVE 60

Query: 61  IPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPHELINQDATTDRYINIPDEVVD 120
           +PRQWYNLVADLPVKPPP LHP T +PIKP+DLS LFP ELI Q+A+TD+YINIPDEVVD
Sbjct: 61  VPRQWYNLVADLPVKPPPPLHPKTLEPIKPEDLSHLFPDELIKQEASTDQYINIPDEVVD 120

Query: 121 IYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVV 180
           IYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQV+Y+VQEGVKNVV
Sbjct: 121 IYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVYYNVQEGVKNVV 180

Query: 181 TETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEG 240
           TETGAGQWG +LAFACS+FG+GC+VWQVRASYDQKPYRR+MM+TWGAKVHPSPSD+T+ G
Sbjct: 181 TETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDMTDAG 240

Query: 241 RKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVLNHVLLHQTVIGEECIKQMEAL 300
           RK LQV+PSSPGSL IAISEA+EVAALNADTKYC+GSVLNHVLLHQTVIGEEC+KQMEAL
Sbjct: 241 RKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEAL 300

Query: 301 GESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAVEPAACPSLTKGVYAYDYADT 360
           GESPDVIIGCTGGGSNFAGLSFPFLREK AGKI+PVIRAVEPAACPSLTKGVYAYDY DT
Sbjct: 301 GESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPVIRAVEPAACPSLTKGVYAYDYGDT 360

Query: 361 ARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQ 420
           A  TPLMKMHTLGHNFIPDPIHA GLRYHGMAPLISHVYNLGLLEAISLPQTECFQGA+Q
Sbjct: 361 AGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQ 420

Query: 421 FARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQGGMI 480
           FAR EGLIPAPE THAIAATI+EALRCRETGESKVILTAM GHGHFDLPAY+KYLQGG+ 
Sbjct: 421 FARSEGLIPAPEPTHAIAATIQEALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGIT 480

Query: 481 DLSFAEEKIQESLTSSIPQL 491
           DLSFA+EKIQESL  +IPQ+
Sbjct: 481 DLSFAKEKIQESLI-TIPQV 497

BLAST of Lcy09g002890 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 740.3 bits (1910), Expect = 9.7e-214
Identity = 354/461 (76.79%), Postives = 405/461 (87.85%), Query Frame = 0

Query: 31  NPNGYRTKAA-SNNPNLKAIEIPRQWYNLVADLPVKPPPSLHPNTFQPIKPQDLSPLFPH 90
           N +  R KAA  +  N   +EIP+QWYNLVADL VKPPP LHP TF+PIKP+DL+ LFP+
Sbjct: 46  NGSSLRVKAALRSTHNKSVVEIPKQWYNLVADLSVKPPPPLHPKTFEPIKPEDLAHLFPN 105

Query: 91  ELINQDATTDRYINIPDEVVDIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSH 150
           ELI Q+AT +R+I+IP+EV++IY LWRPTPLIRAKRLEKLL TPARIY+KYEG SPAGSH
Sbjct: 106 ELIKQEATQERFIDIPEEVLEIYKLWRPTPLIRAKRLEKLLQTPARIYFKYEGGSPAGSH 165

Query: 151 KLNSAVPQVWYHVQEGVKNVVTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRR 210
           K N+AVPQ +Y+ +EGVKNVVTETGAGQWGS+LAFA SLFGL C+VWQV  SY  KPYRR
Sbjct: 166 KPNTAVPQAYYNAKEGVKNVVTETGAGQWGSSLAFASSLFGLDCEVWQVANSYHTKPYRR 225

Query: 211 LMMQTWGAKVHPSPSDLTNEGRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCVGSVL 270
           LMMQTWGAKVHPSPSDLT  GR++L+ +PSSPGSL IAISEA+EVAA N DTKYC+GSVL
Sbjct: 226 LMMQTWGAKVHPSPSDLTEAGRRILESDPSSPGSLGIAISEAVEVAARNEDTKYCLGSVL 285

Query: 271 NHVLLHQTVIGEECIKQMEALGESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRA 330
           NHVLLHQT+IGEECI+QME  GE+PD+IIGCTGGGSNFAGLSFPF+REK  GKI+PVIRA
Sbjct: 286 NHVLLHQTIIGEECIQQMENFGETPDLIIGCTGGGSNFAGLSFPFIREKLKGKINPVIRA 345

Query: 331 VEPAACPSLTKGVYAYDYADTARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVY 390
           VEP+ACPSLTKGVYAYD+ DTA  TPLMKMHTLGH+FIPDPIHA GLRYHGMAPLISHVY
Sbjct: 346 VEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVY 405

Query: 391 NLGLLEAISLPQTECFQGAVQFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTA 450
             G +EAIS+PQ ECFQGA+QFAR EG+IPAPE THAIAATIREALRC+ETGE+KVIL A
Sbjct: 406 EQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCKETGEAKVILMA 465

Query: 451 MSGHGHFDLPAYDKYLQGGMIDLSFAEEKIQESLTSSIPQL 491
           M GHGHFDL +YDKYL+G ++DLSF+EEKI+ESL S +P +
Sbjct: 466 MCGHGHFDLTSYDKYLKGELVDLSFSEEKIRESL-SKVPHV 505

BLAST of Lcy09g002890 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 121.7 bits (304), Expect = 1.6e-27
Identity = 104/342 (30.41%), Postives = 152/342 (44.44%), Query Frame = 0

Query: 136 IYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVTETGAGQWGSALAFACSLFGLGCQV 195
           IY K E ++  G+HK+N+AV Q     + G K ++ ETGAGQ G A A  C+ FGL C +
Sbjct: 156 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCII 215

Query: 196 WQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGRKLLQVEPSSPGSLAIAISEAMEVA 255
           +      +++      M+  GA+V               +   S   +L  A SEA+   
Sbjct: 216 YMGAQDMERQALNVFRMRLLGAEV---------------RGVHSGTATLKDATSEAIRDW 275

Query: 256 ALNAD-TKYCVGSVLN------HVLLHQTVIGEECIKQ-MEALGESPDVIIGCTGGGSNF 315
             N + T Y +GSV         V     VIG+E  KQ ME  G  PDV++ C GGGSN 
Sbjct: 276 VTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGKPDVLVACVGGGSNA 335

Query: 316 AGLSFPFLRE---KFAGKISPVIRAVEPAACPSLTKGVYAYDYADTARTTPLMKMHTLGH 375
            GL   F+ +   +  G  +            +LTKG    D          +     G 
Sbjct: 336 MGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSYLLQDDDGQ 395

Query: 376 NFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQFARCEGLIPAPETT 435
              P  I +AGL Y G+ P  S + ++G  E  S+   E  +   + +R EG+IPA ET+
Sbjct: 396 IIEPHSI-SAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLEGIIPALETS 455

Query: 436 HAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYLQ 467
           HA+A   +    C    +   ++   SG G  D+    KYL+
Sbjct: 456 HALAHLEK---LCPTLPDGARVVLNFSGRGDKDVQTAIKYLE 474

BLAST of Lcy09g002890 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 121.7 bits (304), Expect = 1.6e-27
Identity = 103/341 (30.21%), Postives = 150/341 (43.99%), Query Frame = 0

Query: 136 IYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKNVVTETGAGQWGSALAFACSLFGLGCQV 195
           IY K E ++  G+HK+N+AV Q     + G K ++ ETGAGQ G A A  C+ FGL C +
Sbjct: 151 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECII 210

Query: 196 WQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTNEGRKLLQVEPSSPGSLAIAISEAMEVA 255
           +      +++      M+  GA+V               +   S   +L  A SEA+   
Sbjct: 211 YMGAQDMERQALNVFRMRLLGAEV---------------RGVHSGTATLKDATSEAIRDW 270

Query: 256 ALNAD-TKYCVGSVLN------HVLLHQTVIGEECIKQ-MEALGESPDVIIGCTGGGSNF 315
             N + T Y +GSV         V     VIG+E  KQ +E  G  PDV++ C GGGSN 
Sbjct: 271 VTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNA 330

Query: 316 AGLSFPFLRE---KFAGKISPVIRAVEPAACPSLTKGVYAYDYADTARTTPLMKMHTLGH 375
            GL   F+ +   +  G  +            +LTKG    D          +     G 
Sbjct: 331 MGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSYLLQDDDGQ 390

Query: 376 NFIPDPIHAAGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAVQFARCEGLIPAPETT 435
              P  I +AGL Y G+ P  S   ++G  E  S+   E  +   + +R EG+IPA ET+
Sbjct: 391 IIEPHSI-SAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPALETS 450

Query: 436 HAIAATIREALRCRETGESKVILTAMSGHGHFDLPAYDKYL 466
           HA+A   +    C    +   ++   SG G  D+    KYL
Sbjct: 451 HALAYLEK---LCPTLSDGTRVVLNFSGRGDKDVQTVAKYL 468

BLAST of Lcy09g002890 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.8e-27
Identity = 114/377 (30.24%), Postives = 168/377 (44.56%), Query Frame = 0

Query: 116 RPTPLIRAKRLEK-------LLDTPARIYYKYEGVSPAGSHKLNSAVPQVWYHVQEGVKN 175
           R TPL  A+RL +        ++    IY K E +S  GSHK+N+A+ Q     + G   
Sbjct: 118 RETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSR 177

Query: 176 VVTETGAGQWGSALAFACSLFGLGCQVWQVRASYDQKPYRRLMMQTWGAKVHPSPSDLTN 235
           VV  TGAGQ G A A AC+   L C V+   A  +++ +  L M+  GA+V         
Sbjct: 178 VVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQV--------- 237

Query: 236 EGRKLLQVEPSSPGSLAIAISEAMEVAALNADTKYCV-GSVLNH------VLLHQTVIGE 295
                + VE    G+   A SEA+     N  T Y + G+V+        V   Q+VIG+
Sbjct: 238 -----ISVE----GTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGK 297

Query: 296 ECIKQMEAL-GESPDVIIGCTGGGSNFAGLSFPFLREKFAGKISPVIRAV------EPAA 355
           E  +Q + L G  PDV++ C G GSN  GL   F+ ++    +      +        A 
Sbjct: 298 ETRRQAKQLWGGKPDVLVACVGSGSNALGLFHEFVGDEDVRLVGVEAAGLGLDSGKHSAT 357

Query: 356 CPSLTKGVY--AYDYADTARTTPLMKMHTLGHNFIPDPIHAAGLRYHGMAPLISHVYNLG 415
                 GVY  +  Y        ++K H++G           GL Y G+ P IS +   G
Sbjct: 358 LAFGDVGVYHGSMSYLLQDDQGQILKPHSVG----------VGLEYPGVGPEISFMKETG 417

Query: 416 LLEAISLPQTECFQGAVQFARCEGLIPAPETTHAIAATIREALRCRETGESKVILTAMSG 470
             E  +    E  Q  ++ +R EG+IPA E +HA+A   +     R+   +KV++   SG
Sbjct: 418 RAEFYTATDEEAIQACMRLSRLEGIIPALEASHALAFLDKLVPTLRD--GAKVVVNC-SG 460