Lcy08g006240 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g006240
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionE3 ubiquitin-protein ligase EL5
LocationChr08: 7925248 .. 7928734 (+)
RNA-Seq ExpressionLcy08g006240
SyntenyLcy08g006240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGGAAAGAGTGAATAATTTTTTTTAAAAAAAAACCAAATAAATTTATAGAAAAACATAAAAAAACAAAAACAAAAAACAAAATTCTTTACCTATAGCAGAGCCAAGAGATAATATGGCAGGTCAATTAGTGATCTTACACTAACAAAGTACATAAAATGGTGCTAAATTTCTATCACATACAACACTTCATCAAATAAAATTTGATCCAAAATTACTAGTCTCCAGAATTTCTACATCATGGACTATTTGTTTCCCACGCTGCAGAGAAAATGAAAGTATATTCAAGCAAAGTACTGGATCTCTAAACGGTTTTGTAGGGAGGCTTAAAGTTTTTGCTAACGGCATCTCAATTGTAAACTTTTTTTAAGTTCACGGGTGAGAAGATTTCTTGGTTGAGAATATATATCTTAACTAGTTGGAACTATGCTCTTTTTGGCCATCCTAATGGTAAACTGACAGTATGCAATCCCCACAGATTTGCAAAAATAAGGATTTGATCCAAATTTAAGTAAGAAATTCCATGTTTGCCAACCTGATCTCCAGTCAAATTTTTTGGAAGTCAGAAAAAACAATGAAAATACATGTGTGGTTTTAATGGATCAAAACTTCAATCTTGATAAAAGCTCTCTGCCAGGTTAGTTTTCATGCTTATGGGTAAATAAATGCATTCATTAAGAAACATGCTCAAAACTACATGGGGAGGAATATAGCTGAAAAATGCCATGCAGTTAGGATAAGCATGGAAGGCAGAAAATGCAAAGAGACTTTTATGCTTTAAAGCAAATAAACCGAAAAACTGCACGCGCTGAGGTTGTGGAATAAAGTTTATAGAATCACTTCGAGAGAAAATTGCCCCATCTTCGACGTTGCAGAGCCAAAGATACGAACTTTTCATTAGAATGTTTCTAGAAAAGATGCCTACTTGTCTGGTTGATTACTACTCAACAAAACACACAGGAAAGTTCAAAGATGGAGAGAGAGGTTCTGTGACTGAAGCAAAGACTTGAATTCATTTTCGGGATGTAAATATCCAACAATCTTATAGTGTAAAATCAGCAATGAAAGTGAAGGTTCCACGGAATCAGATTCCTTTCTTTAATTTCAAAGTGAATATTTTCCCCCTAATTTAAAATCTTCAAGCACCTAAATCTGGGAAAAGTAGATCTATAATGGAGTGGGAATCGGACTTTAGTCTTTTACGTGAGAATAAGACAACCAAGCAAAAAAAGAATGCCAAAAAAAAGAAGAAAGGACGGATAAGAGTTGATGGCATGGAGGAGTATATATTCACTTGGATTATCTATTCATCTTCTTGCAGTGAAAGATCTATAAAATCCCAAACACTCAAAACCAACAATCTAATATCTAAAAGATTAAAGAAGAAGAAACTTCTGAAAAATGAGCAGACGTTCTACTAATTTTCCCCCAGGAATTTGGCAGGCTAGAAGGGAATATGATCGCCAGAGATACAGAAATGATTCAAACCGTATGAACTCCACGCCACCAAACCCTCGTTCATCTCAGGTCAGCAGGTTAACAATTCTTTTTTTTTTGTAATGAACTGTATTCAAACCCGCCATCAAAGCTTAAGCAACTATCCTACAACATGATTAGCAGGCAGAAAAACAGAACTTGTAGATTACATTTAAATTCCATGTTAAAAGTTCTCTTAGTTTTTCTCAGAAACATATTAGTAGTTGAGAAGTTAAAGAGATGATAACAATCGTATAGCTCATAATTCATGTGTTGTCAGAATTCACATAGAGAGATAGATCCAGTTCCATGGCGGCCTGCTGGAGAGATAGATCCAGTTCCGTGGCGGCCTCTTGGGGAGATAGATCCAGTTCCGTGGCAGCCGGCTGAGGGGGCAATGATTACAAGAATATTGGACATCGGCCGGTAAATAAAACATAGGCTCAGTAGTTTGATAGGTGCTGTTTGTTTTGGACTGAATTATATGCAAACCAAAGAAGTCGAAATTGCAGATTCATTTCATAAAATGATCTTAAATCATAAGAATTTAGAGGCCAAGCAAAGTTTGTGGTATATTGCCTGAACTAGTATCTAACTCAGGAAGATAACTTGACAGAGCAGTCAGAACTCCATGAATTTCACTAATGGAAGAACTTGATGAAACAAATCATTCAAATCATGAAGTGTCAGAAATGGGGTTGAGCTTCAGGTTCCTTCTTAGTTGGAGACTAGTTCGTTGAAGATTCTTTTACACAAGGGAGTTCAACAGCATTCAACACCACACAACGTATTAAGCATAAATTTGCTCACCAATAATCTCTATTTACAAGTTGCAAGGAAAAAGAAACATGTCATTCTCATTCATTTAATTGAATTACTTATGATCAATAGCTTTCAATTTTTGTCAATAGCAGGATCCAATTTTGCAATAACAGACTAATCCCTTTTGAAAATATCAGTTCGTTTCAGAGATGATCTACTTACATTGCTCTTATTACCTGCAATTAAAATCCGTATCCTCAGTACTGTGTTGATATTCATTCACTTTTTGAGATATTTCCAGCCCGCCGGAAGAACCACCTGCCATAGTCAGAATAATTACCCAATCCAGACCGAGACAGCTCGAAGCCAGTTCAAGACTTGCGGAACTGCACTCGCCAACTCCAAGATCTCAAGGAAACTCCACGCTAACACCTGAAGAGCAAATTATGGCACTGAATAAGCTCAACAAAAAAGTCTATGATCCTCCGAATAACTCAAGTAGGAAAATAAGCTTCTATTACAGAGAGAATAACAGAAGAAATTACAACGAGAGGAGCAACGGCGACAACGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAAGTGATGACGACGCCCTGCAACCATATGTTTCACGAAGAATGCATAGTGCCATGGGTGAAGAGCAATGGCCAATGTCCAGTCTGCAGATTCGCCTTGTTCGAACGAACAGAGCGAAGAGAAGCTTCGTCCAATAATACTGTATCAATTGCGCCGGCCAGTAGCCTGGTCGACGACGATCTCGTGTACTTGATACGAGTCATGGAAGATGCGCTGATGCTGCGAAATCTGGCCGGATTTTGGGGACCATGAAGCGCTATATCGATCTTCCCCTGAAATCAACATGAAGAATGCCCTAAATTTCAAGGATTGATGAAATTCGATCTCTGAAAATTATTGTTGTGCTTTGAACATGAAATAACTCGACGAGAGAGCTCGTTGTGCCCATAAAAGAATCCTGTCGCCGATGGCATTTTCGATCGATTGAGTTTCTTCTTGATTTTGGCGGTATAATTCTTCACGGCCACTGCTGAATGCGATGATCATGAGTTTATAAAAATAATAAAAAATAAGGATGGAATCCTTGGCGCCAAACTTCAGTACTTTTATCTCCGTTATACCTCGAAGACTAAATTATAAAAAATAGGGGCAGTTGCAAGAATAGCAAAAAATAATATGGAATTTGC

mRNA sequence

TATGGAAAGAGTGAATAATTTTTTTTAAAAAAAAACCAAATAAATTTATAGAAAAACATAAAAAAACAAAAACAAAAAACAAAATTCTTTACCTATAGCAGAGCCAAGAGATAATATGGCAGGTCAATTAGTGATCTTACACTAACAAAGTACATAAAATGGTGCTAAATTTCTATCACATACAACACTTCATCAAATAAAATTTGATCCAAAATTACTAGTCTCCAGAATTTCTACATCATGGACTATTTGTTTCCCACGCTGCAGAGAAAATGAAAGTATATTCAAGCAAAGTACTGGATCTCTAAACGGTTTTGTAGGGAGGCTTAAAGTTTTTGCTAACGGCATCTCAATTGTAAACTTTTTTTAAGTTCACGGGTGAGAAGATTTCTTGGTTGAGAATATATATCTTAACTAGTTGGAACTATGCTCTTTTTGGCCATCCTAATGGTAAACTGACAGTATGCAATCCCCACAGATTTGCAAAAATAAGGATTTGATCCAAATTTAAGTAAGAAATTCCATGTTTGCCAACCTGATCTCCAGTCAAATTTTTTGGAAGTCAGAAAAAACAATGAAAATACATGTGTGGTTTTAATGGATCAAAACTTCAATCTTGATAAAAGCTCTCTGCCAGGTTAGTTTTCATGCTTATGGGTAAATAAATGCATTCATTAAGAAACATGCTCAAAACTACATGGGGAGGAATATAGCTGAAAAATGCCATGCAGTTAGGATAAGCATGGAAGGCAGAAAATGCAAAGAGACTTTTATGCTTTAAAGCAAATAAACCGAAAAACTGCACGCGCTGAGGTTGTGGAATAAAGTTTATAGAATCACTTCGAGAGAAAATTGCCCCATCTTCGACGTTGCAGAGCCAAAGATACGAACTTTTCATTAGAATGTTTCTAGAAAAGATGCCTACTTGTCTGGTTGATTACTACTCAACAAAACACACAGGAAAGTTCAAAGATGGAGAGAGAGGTTCTGTGACTGAAGCAAAGACTTGAATTCATTTTCGGGATGTAAATATCCAACAATCTTATAGTGTAAAATCAGCAATGAAAGTGAAGGTTCCACGGAATCAGATTCCTTTCTTTAATTTCAAAGTGAATATTTTCCCCCTAATTTAAAATCTTCAAGCACCTAAATCTGGGAAAAGTAGATCTATAATGGAGTGGGAATCGGACTTTAGTCTTTTACGTGAGAATAAGACAACCAAGCAAAAAAAGAATGCCAAAAAAAAGAAGAAAGGACGGATAAGAGTTGATGGCATGGAGGAGTATATATTCACTTGGATTATCTATTCATCTTCTTGCAGTGAAAGATCTATAAAATCCCAAACACTCAAAACCAACAATCTAATATCTAAAAGATTAAAGAAGAAGAAACTTCTGAAAAATGAGCAGACGTTCTACTAATTTTCCCCCAGGAATTTGGCAGGCTAGAAGGGAATATGATCGCCAGAGATACAGAAATGATTCAAACCGTATGAACTCCACGCCACCAAACCCTCGTTCATCTCAGAATTCACATAGAGAGATAGATCCAGTTCCATGGCGGCCTGCTGGAGAGATAGATCCAGTTCCGTGGCGGCCTCTTGGGGAGATAGATCCAGTTCCGTGGCAGCCGGCTGAGGGGGCAATGATTACAAGAATATTGGACATCGGCCGCCCGCCGGAAGAACCACCTGCCATAGTCAGAATAATTACCCAATCCAGACCGAGACAGCTCGAAGCCAGTTCAAGACTTGCGGAACTGCACTCGCCAACTCCAAGATCTCAAGGAAACTCCACGCTAACACCTGAAGAGCAAATTATGGCACTGAATAAGCTCAACAAAAAAGTCTATGATCCTCCGAATAACTCAAGTAGGAAAATAAGCTTCTATTACAGAGAGAATAACAGAAGAAATTACAACGAGAGGAGCAACGGCGACAACGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAAGTGATGACGACGCCCTGCAACCATATGTTTCACGAAGAATGCATAGTGCCATGGGTGAAGAGCAATGGCCAATGTCCAGTCTGCAGATTCGCCTTGTTCGAACGAACAGAGCGAAGAGAAGCTTCGTCCAATAATACTGTATCAATTGCGCCGGCCAGTAGCCTGGTCGACGACGATCTCGTGTACTTGATACGAGTCATGGAAGATGCGCTGATGCTGCGAAATCTGGCCGGATTTTGGGGACCATGAAGCGCTATATCGATCTTCCCCTGAAATCAACATGAAGAATGCCCTAAATTTCAAGGATTGATGAAATTCGATCTCTGAAAATTATTGTTGTGCTTTGAACATGAAATAACTCGACGAGAGAGCTCGTTGTGCCCATAAAAGAATCCTGTCGCCGATGGCATTTTCGATCGATTGAGTTTCTTCTTGATTTTGGCGGTATAATTCTTCACGGCCACTGCTGAATGCGATGATCATGAGTTTATAAAAATAATAAAAAATAAGGATGGAATCCTTGGCGCCAAACTTCAGTACTTTTATCTCCGTTATACCTCGAAGACTAAATTATAAAAAATAGGGGCAGTTGCAAGAATAGCAAAAAATAATATGGAATTTGC

Coding sequence (CDS)

ATGAGCAGACGTTCTACTAATTTTCCCCCAGGAATTTGGCAGGCTAGAAGGGAATATGATCGCCAGAGATACAGAAATGATTCAAACCGTATGAACTCCACGCCACCAAACCCTCGTTCATCTCAGAATTCACATAGAGAGATAGATCCAGTTCCATGGCGGCCTGCTGGAGAGATAGATCCAGTTCCGTGGCGGCCTCTTGGGGAGATAGATCCAGTTCCGTGGCAGCCGGCTGAGGGGGCAATGATTACAAGAATATTGGACATCGGCCGCCCGCCGGAAGAACCACCTGCCATAGTCAGAATAATTACCCAATCCAGACCGAGACAGCTCGAAGCCAGTTCAAGACTTGCGGAACTGCACTCGCCAACTCCAAGATCTCAAGGAAACTCCACGCTAACACCTGAAGAGCAAATTATGGCACTGAATAAGCTCAACAAAAAAGTCTATGATCCTCCGAATAACTCAAGTAGGAAAATAAGCTTCTATTACAGAGAGAATAACAGAAGAAATTACAACGAGAGGAGCAACGGCGACAACGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAAGTGATGACGACGCCCTGCAACCATATGTTTCACGAAGAATGCATAGTGCCATGGGTGAAGAGCAATGGCCAATGTCCAGTCTGCAGATTCGCCTTGTTCGAACGAACAGAGCGAAGAGAAGCTTCGTCCAATAATACTGTATCAATTGCGCCGGCCAGTAGCCTGGTCGACGACGATCTCGTGTACTTGATACGAGTCATGGAAGATGCGCTGATGCTGCGAAATCTGGCCGGATTTTGGGGACCATGA

Protein sequence

MSRRSTNFPPGIWQARREYDRQRYRNDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEIDPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAELHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGDNDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREASSNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWGP
Homology
BLAST of Lcy08g006240 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 29/58 (50.00%), Postives = 36/58 (62.07%), Query Frame = 0

Query: 187 CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREASSNN 245
           CAVC E F+ + E    PC H+FH++CIVPW+     CPVCRF L     RR  S+NN
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNN 254

BLAST of Lcy08g006240 vs. ExPASy Swiss-Prot
Match: Q9Y4L5 (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 31/69 (44.93%), Postives = 42/69 (60.87%), Query Frame = 0

Query: 184 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL-----FERTERR 243
           G  C VC ED+  +EEV   PCNH FH  CIVPW++ +  CPVCR +L       +++  
Sbjct: 225 GLECPVCKEDYTVEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQSQST 284

Query: 244 EASSNNTVS 248
           EAS++N  S
Sbjct: 285 EASASNRFS 293

BLAST of Lcy08g006240 vs. ExPASy Swiss-Prot
Match: Q9D0C1 (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 31/69 (44.93%), Postives = 42/69 (60.87%), Query Frame = 0

Query: 184 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL-----FERTERR 243
           G  C VC ED+  +E+V   PCNH FH  CIVPW++ +  CPVCR +L       +T+  
Sbjct: 226 GLECPVCKEDYTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQTQSS 285

Query: 244 EASSNNTVS 248
           EAS++N  S
Sbjct: 286 EASASNRFS 294

BLAST of Lcy08g006240 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-10
Identity = 26/67 (38.81%), Postives = 44/67 (65.67%), Query Frame = 0

Query: 181 DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREA 240
           DEEG+ C +C+E F+  ++V+  PC H FHE CI PW++ NG C +CR  +   +++R  
Sbjct: 391 DEEGE-CTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPVDPNSQQRNN 450

Query: 241 SSNNTVS 248
           +S ++ +
Sbjct: 451 TSTDSAN 456

BLAST of Lcy08g006240 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.3e-10
Identity = 22/49 (44.90%), Postives = 36/49 (73.47%), Query Frame = 0

Query: 183 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 232
           E  +CAVC+++F+   +V   PC H+FH++C++PW++ +  CPVCRF L
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of Lcy08g006240 vs. ExPASy TrEMBL
Match: A0A6J1JPC8 (E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.9e-121
Identity = 219/281 (77.94%), Postives = 244/281 (86.83%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRYR-NDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPG+W+ARREYDRQRY  NDSNRM+ST P+P+  QNS R+ +P+P RP   I
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
           DPVP RP  E+DP+PW+P  GAMI R+L+I    E+PPAIVRIIT SRPRQ+EASSR AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+ PTPRS+GNSTLT EEQ MALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNER NG+
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           ASSNNT SIA  S+LVDD+LVYLIR MED+LMLRNL G WG
Sbjct: 241 ASSNNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

BLAST of Lcy08g006240 vs. ExPASy TrEMBL
Match: A0A6J1ES11 (E3 ubiquitin-protein ligase EL5 OS=Cucurbita moschata OX=3662 GN=LOC111437204 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.3e-121
Identity = 218/281 (77.58%), Postives = 243/281 (86.48%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRYR-NDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRR TNFPPG+W+ARREYD QRY  NDSNRM+ST P+P+  QNS R+ +P+P RP G I
Sbjct: 1   MSRRPTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPSRPTGGI 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
           DPVP RP  E+DP+PW+P  GAMI R+L+I    E+PPAIVRIIT SRPRQ+EASSR AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+ PTPRS+GNSTLT EEQ MALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNERSNG+
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERSNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           ASSNN  SIA  S+LVDD+LVYLIR MED+LMLRNL G WG
Sbjct: 241 ASSNNAASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

BLAST of Lcy08g006240 vs. ExPASy TrEMBL
Match: A0A1S3C054 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495488 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.4e-111
Identity = 216/284 (76.06%), Postives = 235/284 (82.75%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRY-RNDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRY RN+SNR+NSTP + +S QNS+REIDPVPWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGE- 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
                                 MITRILDIG  PEEPPAIVRIIT SRPRQ+EAS   AE
Sbjct: 61  ---------------------TMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+SPTPRS+GNSTLT EEQ MALNKL KKVYDPP+N+SR+I+FYYRENNR NYNER N +
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTERR 240
           +DEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RTERR
Sbjct: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERR 240

Query: 241 EASS--NNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
            ASS  NN VSIAP SSLVDD+L+YLIR ME+ALMLRN+A FWG
Sbjct: 241 GASSNFNNNVSIAPPSSLVDDELMYLIRAMEEALMLRNVAEFWG 262

BLAST of Lcy08g006240 vs. ExPASy TrEMBL
Match: A0A5D3C526 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003170 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.2e-110
Identity = 215/283 (75.97%), Postives = 235/283 (83.04%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRY-RNDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRY RN+SNR+NSTP + +S QNS+REIDPVPWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGE- 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
                                 MITRILDIG  PEEPPAIVRIIT SRPRQ+EAS   AE
Sbjct: 61  ---------------------TMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+SPTPRS+GNSTLT EEQ MALNKL KKVYDPP+N+SR+I+FYYRENNR NYNER N +
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTERR 240
           +DEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RTERR
Sbjct: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERR 240

Query: 241 EASS--NNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFW 280
            ASS  NN+VSIAP SSLVDD+L+YLIR ME+ALMLRN+A FW
Sbjct: 241 GASSNFNNSVSIAPPSSLVDDELMYLIRAMEEALMLRNVAEFW 261

BLAST of Lcy08g006240 vs. ExPASy TrEMBL
Match: A0A0A0K1V1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.4e-110
Identity = 212/284 (74.65%), Postives = 234/284 (82.39%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRY-RNDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPGIWQARRE+DRQRY RN+SNR NSTP + +S QNS+R+IDPVPWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRTNSTPSHTQSFQNSYRQIDPVPWRPAGE- 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
                                 +ITRILDIG  PEEPPAIVRIIT SRPRQLEAS   AE
Sbjct: 61  ---------------------TVITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+SPTPRS+GNSTLT EEQ MALNKL KKVYDPP+N+SR+ISFYYRENNR N NER N +
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
            DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKS+GQCPVCRF LFERTERR 
Sbjct: 181 GDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRG 240

Query: 241 ASS---NNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           ++S   NN+VSIAP SSLVD++LVYLIR ME+ALMLRN+A FWG
Sbjct: 241 SASSNFNNSVSIAPPSSLVDEELVYLIRAMEEALMLRNVAEFWG 262

BLAST of Lcy08g006240 vs. NCBI nr
Match: XP_023529368.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 446.4 bits (1147), Expect = 1.8e-121
Identity = 218/281 (77.58%), Postives = 245/281 (87.19%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRYR-NDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPG+W+ARREYD QRY  NDSNRM+ST P+P+  QNS R+ +P+P RP G I
Sbjct: 1   MSRRSTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPLRPTGGI 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
           DPVP RP  E+DP+PW+P  GAMI R+L+I    E+PPAIVRIIT SRPRQ+EASSR AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           ++ PTPRS+GNSTLT EEQ MALNKL KKVYDPPNN+SR+ISFYYRENNR+NYNERSNG+
Sbjct: 121 MYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRKNYNERSNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           ASSNNT SIA  S+LVDD+LVYLIR MED+LMLRNL G WG
Sbjct: 241 ASSNNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

BLAST of Lcy08g006240 vs. NCBI nr
Match: XP_022989128.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita maxima])

HSP 1 Score: 445.3 bits (1144), Expect = 4.0e-121
Identity = 219/281 (77.94%), Postives = 244/281 (86.83%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRYR-NDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPG+W+ARREYDRQRY  NDSNRM+ST P+P+  QNS R+ +P+P RP   I
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
           DPVP RP  E+DP+PW+P  GAMI R+L+I    E+PPAIVRIIT SRPRQ+EASSR AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+ PTPRS+GNSTLT EEQ MALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNER NG+
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           ASSNNT SIA  S+LVDD+LVYLIR MED+LMLRNL G WG
Sbjct: 241 ASSNNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

BLAST of Lcy08g006240 vs. NCBI nr
Match: XP_022930836.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita moschata] >KAG7022947.1 E3 ubiquitin-protein ligase SDIR1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 444.1 bits (1141), Expect = 8.8e-121
Identity = 218/281 (77.58%), Postives = 243/281 (86.48%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRYR-NDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRR TNFPPG+W+ARREYD QRY  NDSNRM+ST P+P+  QNS R+ +P+P RP G I
Sbjct: 1   MSRRPTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPSRPTGGI 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
           DPVP RP  E+DP+PW+P  GAMI R+L+I    E+PPAIVRIIT SRPRQ+EASSR AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+ PTPRS+GNSTLT EEQ MALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNERSNG+
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERSNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           ASSNN  SIA  S+LVDD+LVYLIR MED+LMLRNL G WG
Sbjct: 241 ASSNNAASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

BLAST of Lcy08g006240 vs. NCBI nr
Match: XP_038888228.1 (uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida])

HSP 1 Score: 427.6 bits (1098), Expect = 8.6e-116
Identity = 221/283 (78.09%), Postives = 235/283 (83.04%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRY-RNDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRY RNDSNR+NSTP +  S QNS+RE+DPVPWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRINSTPSHTNSFQNSYREVDPVPWRPAGE- 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
                                 MI RILDIGRP EEPPAIVRIIT SRPRQLEAS   AE
Sbjct: 61  ---------------------TMIARILDIGRPREEPPAIVRIITSSRPRQLEASPIPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+SPTPRS+GNSTLT EEQ MAL KL K VYDPP+N+SR+ISFYYRENNR NYNER+N +
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALTKLKKNVYDPPSNTSRRISFYYRENNRGNYNERNNSN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           +DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRF LFERTERR 
Sbjct: 181 SDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTERRG 240

Query: 241 AS--SNNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
           AS  SNNTVS AP SSL+DDDL+YLIR MEDALMLRNLAGFWG
Sbjct: 241 ASSNSNNTVSTAPPSSLIDDDLMYLIRAMEDALMLRNLAGFWG 261

BLAST of Lcy08g006240 vs. NCBI nr
Match: XP_008455285.1 (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo])

HSP 1 Score: 411.8 bits (1057), Expect = 4.9e-111
Identity = 216/284 (76.06%), Postives = 235/284 (82.75%), Query Frame = 0

Query: 1   MSRRSTNFPPGIWQARREYDRQRY-RNDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRY RN+SNR+NSTP + +S QNS+REIDPVPWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGE- 60

Query: 61  DPVPWRPLGEIDPVPWQPAEGAMITRILDIGRPPEEPPAIVRIITQSRPRQLEASSRLAE 120
                                 MITRILDIG  PEEPPAIVRIIT SRPRQ+EAS   AE
Sbjct: 61  ---------------------TMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAE 120

Query: 121 LHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGD 180
           L+SPTPRS+GNSTLT EEQ MALNKL KKVYDPP+N+SR+I+FYYRENNR NYNER N +
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTERR 240
           +DEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RTERR
Sbjct: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERR 240

Query: 241 EASS--NNTVSIAPASSLVDDDLVYLIRVMEDALMLRNLAGFWG 281
            ASS  NN VSIAP SSLVDD+L+YLIR ME+ALMLRN+A FWG
Sbjct: 241 GASSNFNNNVSIAPPSSLVDDELMYLIRAMEEALMLRNVAEFWG 262

BLAST of Lcy08g006240 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.7e-29
Identity = 71/182 (39.01%), Postives = 102/182 (56.04%), Query Frame = 0

Query: 100 VRIITQSRPRQLEASSRLAELHSPTPRSQGNSTLTPEEQIMALNKLNKKVYDPPNNSS-- 159
           +R     R + LEA  +     S  P S   S+    E+   L  L K+ Y+P   S+  
Sbjct: 53  IRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLL 112

Query: 160 RKISFYYRENNRRNYNERS--NGDNDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIV 219
           R +S YYR  N  + + R+  N   +E+ KRC+VCLEDF+PKE VM TPC HMFHEECIV
Sbjct: 113 RSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIV 172

Query: 220 PWVKSNGQCPVCRFALFERTERRE---ASSNNTVSIAPASSLVDDDLVYLIRVMEDALML 275
           PW+K+ GQCPVCRF + + T++     A+ +N       + L   +L+ ++R ME+  + 
Sbjct: 173 PWLKTKGQCPVCRFVILKPTKQDSSPPANGSNLTGDMTTNDLFTLELLSVVRAMEETFLF 232

BLAST of Lcy08g006240 vs. TAIR 10
Match: AT3G58720.2 (RING/U-box superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-28
Identity = 88/264 (33.33%), Postives = 127/264 (48.11%), Query Frame = 0

Query: 26  NDSNRMNSTPPNPRSSQNSHREIDPVPWRPAGEIDPVPWRPLGEI-------DPVPWQPA 85
           N+ N  +S P  P  S +S  +     WR         W     I       DP+     
Sbjct: 3   NNRNGYSSVPNQPNYSYDS--DTRSSGWRVPAFSSRNAWSRSNSIVNRHLIDDPI--SAY 62

Query: 86  EGAMITRILDIGRPPEEPPAI-VRIITQSRPRQLEASSRLAELHSPTPRSQGNSTLTPEE 145
               I+  +D+  P      I +R     R + LEA  +     S  P S   S+    E
Sbjct: 63  SRTSISSTVDLVPPSHTRHQIPIRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNE 122

Query: 146 QIMALNKLNKKVYDPPNNSS--RKISFYYRENNRRNYNERS--NGDNDEEGKRCAVCLED 205
           +   L  L K+ Y+P   S+  R +S YYR  N  + + R+  N   +E+ KRC+VCLED
Sbjct: 123 EDEILKHLTKETYNPVPKSTLLRSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLED 182

Query: 206 FQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE---ASSNNTVSIAP 265
           F+PKE VM TPC HMFHEECIVPW+K+ GQCPVCRF + + T++     A+ +N      
Sbjct: 183 FEPKETVMLTPCKHMFHEECIVPWLKTKGQCPVCRFVILKPTKQDSSPPANGSNLTGDMT 242

Query: 266 ASSLVDDDLVYLIRVMEDALMLRN 275
            + L   +L+ ++R ME+  +  N
Sbjct: 243 TNDLFTLELLSVVRAMEETFLFGN 262

BLAST of Lcy08g006240 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-12
Identity = 29/58 (50.00%), Postives = 36/58 (62.07%), Query Frame = 0

Query: 187 CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREASSNN 245
           CAVC E F+ + E    PC H+FH++CIVPW+     CPVCRF L     RR  S+NN
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNN 254

BLAST of Lcy08g006240 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-11
Identity = 22/49 (44.90%), Postives = 36/49 (73.47%), Query Frame = 0

Query: 183 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 232
           E  +CAVC+++F+   +V   PC H+FH++C++PW++ +  CPVCRF L
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of Lcy08g006240 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-11
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 0

Query: 178 GDNDEEGKR-CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL----F 237
           G+ND++G   CAVC ++     + +  PCNH +H ECIVPW+K    CPVCR+ L     
Sbjct: 299 GENDDDGGLVCAVCKDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDDA 358

Query: 238 ERTERREASSNNT 246
           E  +R+   + NT
Sbjct: 359 EYEQRKTQRTTNT 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q940T52.7e-1150.00E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Q9Y4L52.7e-1144.93E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2[more]
Q9D0C12.7e-1144.93E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1[more]
Q9C1X41.0e-1038.81Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra... [more]
Q8LPN72.3e-1044.90E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Match NameE-valueIdentityDescription
A0A6J1JPC81.9e-12177.94E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4... [more]
A0A6J1ES114.3e-12177.58E3 ubiquitin-protein ligase EL5 OS=Cucurbita moschata OX=3662 GN=LOC111437204 PE... [more]
A0A1S3C0542.4e-11176.06E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5D3C5261.2e-11075.97E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0K1V14.4e-11074.65RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 P... [more]
Match NameE-valueIdentityDescription
XP_023529368.11.8e-12177.58E3 ubiquitin-protein ligase EL5 [Cucurbita pepo subsp. pepo][more]
XP_022989128.14.0e-12177.94E3 ubiquitin-protein ligase EL5 [Cucurbita maxima][more]
XP_022930836.18.8e-12177.58E3 ubiquitin-protein ligase EL5 [Cucurbita moschata] >KAG7022947.1 E3 ubiquitin-... [more]
XP_038888228.18.6e-11678.09uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida][more]
XP_008455285.14.9e-11176.06PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G58720.11.7e-2939.01RING/U-box superfamily protein [more]
AT3G58720.23.2e-2833.33RING/U-box superfamily protein [more]
AT5G59550.11.9e-1250.00zinc finger (C3HC4-type RING finger) family protein [more]
AT3G19950.11.6e-1144.90RING/U-box superfamily protein [more]
AT5G08139.11.6e-1141.10RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 187..227
e-value: 1.8E-9
score: 47.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 186..228
e-value: 3.6E-14
score: 52.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 187..228
score: 13.536741
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 171..247
e-value: 1.1E-21
score: 77.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..134
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 138..274
NoneNo IPR availablePANTHERPTHR22765:SF328ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEINcoord: 138..274
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 187..228
e-value: 1.30805E-19
score: 78.1125
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 179..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g006240.1Lcy08g006240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
molecular_function GO:0061630 ubiquitin protein ligase activity