Lcy08g000620 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g000620
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCN hydrolase domain-containing protein
LocationChr08: 1039475 .. 1044019 (+)
RNA-Seq ExpressionLcy08g000620
SyntenyLcy08g000620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGACCTCGCAGACTGACGCTGCAAATGGAGAGCGTAACGATGGAAAACACGCCATTAACAGTGCCTCGGTCTGTGGTTACGACTCGCTCCATCAACTCCTTGCTGAAAATCTCAAACCTCAGATTTTCCAGGTCGTTTTCTCTGTTTTTTTTTTTTTTATCGATTTTGTCCGTGTGGCTAGAAAAATTGTTAGTGAAGTATACAAGCGATCGTGAAAAATTGTTAATGACCTGTTTGTGGAGGTCGATTGTTTTTTGCGCATTTCGTTGAGCTCTGTTTGATTCCGTACTGTTGGGCGGTGGATTATGTGAATCGGGAATCTTTTCGTACTTTATCTGAGAGATGCTTGTAAGTTTTTGGATGTAGTAGGTTTTAAACAATCAGCCGCTTAAACATTTCGGTTTCGAAATTTAGTTGTTAATTATTGTTGTTACCCGTGTGGCTAGAAAAATTGTTAGTGAAGTATATAAGCGATCATGAAAAATTGTTAATGATCTGTTTGGGAATGTCGATTGTTTTTGCTCATTTCGTAGAGTTCTGTTTGATTCCGTACTGTTGGGCAGTGGATTCTGTGAATCGGGAATATTTTCGTACTTTATCTGAGAGATGCTTGAAAGTTTTTTTGGATGTAGTAGGTTTAATCAATCAGCCGCTTAAACATTTCGGTTTCGAAATTTAGTTGTTAATTATTGCTGTTACCCGTGTGGCTAGAAAAATTGTTAGTGAAGTATATAAGCGATCATGAAAAATTGTTAATGATCTGTTTGTGAATGTCGATTGTTTTTGCTCATTTCGTAGAGTTCTGTTTGATTCCGTACTGTTGGGCAGTGGATTCTGTGAATCGGGAATATTTTCGTACTTTATCTGAGAGATGCTTGAAAGTTTTTGGATGTAGTAGGTTTAATCAATCAGCCGCTTAAACACTTCGGTTTCGAAATTTAGTTGTTAATTATTGTTGTTACTCTGTTGTTGGTTGAAAAGGTTTGAAAATCACGATGTGAATTATATCCAATGGAGATTGGTATGAATTTTTGGCTGAAGAGCCTTAACTGAACAAGCTAGCTGGAATAGGTAGATGCGAATCCTGCAGTAGCTTTATACAAGGACTATTGAATTCTATGTCAACGGATTCTCTGAGCACACTTTTTTGCTCATGTATTTGTCTTTAAAACCATACTTATTTACCAACAATTTTTTCTTTATTTTTTAATTACTTCCGACCCAAAACTTGAAACATACAAAAAAAAAAAGTAGGTTAAAAAACATGTTTGGTTTTTAGAATTTGACAAAGATTGCAAAGGTGGTTTTAAAAATTTAAAAATAAAATAAAATAAATATAGGTATATATAGTTTTCATAAGCTTAATTTTTTTAAAAAAAATATAAAACCATTGGCAAATTCATTATTAAATAGGGTCGTGAAGGTTTATTAGTCAACAATGTCACAGGGTGCTAAGATTGTCTGATCCACGTTGCAAAGTAGCTAAAAAACACATAAAAATCATGTTGTGTATTTACTAGTTTTTATGTGGCCAACGTGTCTGACTTTGCTCCATGCTGTAGCTATTCATGTTTCTTTCAATTACTTTTCTATTTCGTAGCCTAACAACTACATTTGTTCATTATATTATTCATATGATCTTCATTTATCTAGAAATTACAAATTTAGCATTTTTAGCATCTCCTGAAACATTGGACAAAATCAGTCCAAAATTTCTTTTCTCCTCAGACCAACGTATTCCAAAGCAACATAGATTTTTTTTCTGGTATGCATGCTTCAAGTATTTCTTTGTTTACCCATTAAAAAAAAGTAGTTTTTTTTTTTTTTTTTTATTTTTTTTTTATTTTTTTATTTTTTTATTTATTTATTTACTTTGACCTTTTGAAATATACAACTAACTTCATCATTGCACTGCCAATACCAGGAAGTTAGCCGGTTGCTATTAGGGCTTAACTGTGGGAAGCCGCTTCAAACTATTTCTCTTGCAGAGCCTGTGAAGAGTCTTTCTCTAAAGCATGATTTTGACCTCCAGGTAGTCACCTGTAGGAATCCCTTATTAAGTTTCTTTGATAACATTGATTACATGTTATTTGCAATGCAGAAACCATATTAATATTTGTGCCTGCTTTACTATAAATCTGAGTTTATGAGTTGATTGCAGAGATTTGTTTTGCCAGAAGCAGTCAGCAATCTCTATCTTCTATTGAACAAAACTTGGCTGCATACCGGTATTGACAAATAAGAAAGAGAAAGATAGAAAATGACAACTTTTTTATCTCTCCTAGTTGTTAACACTTTAATGGTGGTATGTAGCGAAATCCCTACATACCGGTATGCAGCCAAGTTTTTTCCTCTTTTATTTTTATTGTTTAGTTGTGATGTTTTCATGTTGGTAACCCATTTTTTCCATGTTGAAGTACATTGAAAGCGATGAATTAAAGGTTTGTCTTTTATGGAGCACACTGAAGAAGTAATGGGAATCAATAAAGGAAAATTAAGAAGTTTTGAGCTGATGTAGTTCTTACATATTCTTTGTTTGTATTTTTCCTATAGGATCGGAGGTCAACATGGGCTGTGTAGTCTTTTATAATTTACATTTATTGCTCTCTTGCCCTCTCTTTCACAAAAATAAAAAGAGAAAGATTAAAATAATTCAAATGTATGATAGCAAGTCAGACCTCTTTGTGGTCATTCCAACACATTGGTTATTTAACAAAGGGGAGGTGATGCGTGTAATATTAGGATACAATGAGGTTTCTGTGGCATTTGAAAGCCTCTCTCTGCAAATCTTATAAGAAAAGGCTTCTCTCTGCAAAAGATTATTGTGGCCTACAGACTTCTATACTGATCTATGTCATACCAATTATTTTATTCATCGTGCAACATTTATGTGATGAATGGCGCTCATGGTTTTTTTTTTTATTATTATTTTTTATTTATTTATTTATTTATTTTTTTTTTTTATTTCTATGACATTTTATTGATGTTTATACCTATTATTCTTTTATGATTTCAGGCTTTCCGCTTTCATGCTGACAAGGAATCAGTGAGAGAACCTCGAATAGTAAGGGTGGGTCTCATTCAAAATTCAATTGCTCTTCCAACAACTGCCCCATTCTCAGACCAAAAGAGGGCTATTCTTGACAAAGTAAAGCCAATTATTGATGCAGCAGGTGCTTCTGGAGTCAATATATTATGCTTGCAAGTAGGTTACGGTGGCCAACTGTATTTCTACTTATATTCTTTGACGTTTTGGAGCTTTCTATATTCATACATATATATATATGTAGGAAGCATGGATGATGCCATTTGCTTTTTGCACACGTGAGAAGCGGTGGTGTGAATTTGCAGAGCCAATTGATGGGGAATCGACACAGTTTCTTCAGGATCTTGCGTTAAGATACAACATGGTCATTGTAAGTTCCATTCTTGAGAGAGATGTTAATCATGGAGAAACTTTGTGGAATACTGCTGTTGTTATTGGAAATCATGGAAACATAATTGGCAAGCATCGCAAGGTAACCGTTGTTTATGGAGTTAGTTTTCATATCACACTCTCTCGGCTGTTGAGACTATTTTGGTTAATGGCATTTTGCAGAACCATATACCAAGAGTTGGTGACTTTAACGAGAGCACGTACTATATGGAAGGAAACACCGGGCATCCTGTGTTTGAGACAGCCTACGGGAAAATTGCAATAAATATATGTTACGGGAGACACCACCCTTTGAACTGGTTAGCTTTCGGCTTCAATGGTGCTGAGATTGTTTTCAACCCTTCTGCGACTGTTGGTGAACTCAGTGAACCAATGTGGCCTATTGAGGTTAGTCTTTTACTTGTTTCACCCAGTTCTCATTTGTTTCAAGTAATCATTTCACTTATACCACAAAAGTCAAACAAGGCAAATGAATGCAGATAATTGTTCTTTTTCCTTTGATTTATAACTGCAAATTGCGATGTTCCATTTTTTTTTCCTTTGGCTTAGATGCTTCAACTTTGGCCTATTACGTTGTTCGACTGAGTTGCAAATTACGAAACTGGTTATACTGTTATCGTTGTATGAGCAGTCTGTTACTATACTAGTTCTACCAACTTCTATAGAAAACTCATACTTTTATCTGCAATCTATTATTACCATTTAAAATTACGATGGACATATTCTGTGTTTGCAGGCCCGAAATGCTGCAATAGCAAATAGTTATTTTGTTGGATCGATCAATCGTGTCGGGACCGAGATTTTCCCCAATCCATTCACGTCAGGTGATGGGAAACCACAGCATACAGATTTTGGTCACTTTTACGGGTCCAGCCATTTCTCTGCACCAGATGCCTCTTGCACTCCCTCACTCTCCCGTTTTCGGGATGGTTTATTAATCTCCGACATGGATCTTAACTTGTGTAGGCAGCTGAAAGATAAATGGGGATTTCGAATGACTGCTCGGTACGAGTTGTATGCTGAAATGCTTGCTCGGTATCTGAAGCCTGACTTTGAGCCTCAAGTCATTTCTGATCCCTTGTTACACAAGAAGGCTTCTTGA

mRNA sequence

ATGGAGAAGACCTCGCAGACTGACGCTGCAAATGGAGAGCGTAACGATGGAAAACACGCCATTAACAGTGCCTCGGTCTGTGGTTACGACTCGCTCCATCAACTCCTTGCTGAAAATCTCAAACCTCAGATTTTCCAGGAAGTTAGCCGGTTGCTATTAGGGCTTAACTGTGGGAAGCCGCTTCAAACTATTTCTCTTGCAGAGCCTGTGAAGAGTCTTTCTCTAAAGCATGATTTTGACCTCCAGGCTTTCCGCTTTCATGCTGACAAGGAATCAGTGAGAGAACCTCGAATAGTAAGGGTGGGTCTCATTCAAAATTCAATTGCTCTTCCAACAACTGCCCCATTCTCAGACCAAAAGAGGGCTATTCTTGACAAAGTAAAGCCAATTATTGATGCAGCAGGTGCTTCTGGAGTCAATATATTATGCTTGCAAGAAGCATGGATGATGCCATTTGCTTTTTGCACACGTGAGAAGCGGTGGTGTGAATTTGCAGAGCCAATTGATGGGGAATCGACACAGTTTCTTCAGGATCTTGCGTTAAGATACAACATGGTCATTGTAAGTTCCATTCTTGAGAGAGATGTTAATCATGGAGAAACTTTGTGGAATACTGCTGTTGTTATTGGAAATCATGGAAACATAATTGGCAAGCATCGCAAGAACCATATACCAAGAGTTGGTGACTTTAACGAGAGCACGTACTATATGGAAGGAAACACCGGGCATCCTGTGTTTGAGACAGCCTACGGGAAAATTGCAATAAATATATGTTACGGGAGACACCACCCTTTGAACTGGTTAGCTTTCGGCTTCAATGGTGCTGAGATTGTTTTCAACCCTTCTGCGACTGTTGGTGAACTCAGTGAACCAATGTGGCCTATTGAGGCCCGAAATGCTGCAATAGCAAATAGTTATTTTGTTGGATCGATCAATCGTGTCGGGACCGAGATTTTCCCCAATCCATTCACGTCAGGTGATGGGAAACCACAGCATACAGATTTTGGTCACTTTTACGGGTCCAGCCATTTCTCTGCACCAGATGCCTCTTGCACTCCCTCACTCTCCCGTTTTCGGGATGGTTTATTAATCTCCGACATGGATCTTAACTTGTGTAGGCAGCTGAAAGATAAATGGGGATTTCGAATGACTGCTCGGTACGAGTTGTATGCTGAAATGCTTGCTCGGTATCTGAAGCCTGACTTTGAGCCTCAAGTCATTTCTGATCCCTTGTTACACAAGAAGGCTTCTTGA

Coding sequence (CDS)

ATGGAGAAGACCTCGCAGACTGACGCTGCAAATGGAGAGCGTAACGATGGAAAACACGCCATTAACAGTGCCTCGGTCTGTGGTTACGACTCGCTCCATCAACTCCTTGCTGAAAATCTCAAACCTCAGATTTTCCAGGAAGTTAGCCGGTTGCTATTAGGGCTTAACTGTGGGAAGCCGCTTCAAACTATTTCTCTTGCAGAGCCTGTGAAGAGTCTTTCTCTAAAGCATGATTTTGACCTCCAGGCTTTCCGCTTTCATGCTGACAAGGAATCAGTGAGAGAACCTCGAATAGTAAGGGTGGGTCTCATTCAAAATTCAATTGCTCTTCCAACAACTGCCCCATTCTCAGACCAAAAGAGGGCTATTCTTGACAAAGTAAAGCCAATTATTGATGCAGCAGGTGCTTCTGGAGTCAATATATTATGCTTGCAAGAAGCATGGATGATGCCATTTGCTTTTTGCACACGTGAGAAGCGGTGGTGTGAATTTGCAGAGCCAATTGATGGGGAATCGACACAGTTTCTTCAGGATCTTGCGTTAAGATACAACATGGTCATTGTAAGTTCCATTCTTGAGAGAGATGTTAATCATGGAGAAACTTTGTGGAATACTGCTGTTGTTATTGGAAATCATGGAAACATAATTGGCAAGCATCGCAAGAACCATATACCAAGAGTTGGTGACTTTAACGAGAGCACGTACTATATGGAAGGAAACACCGGGCATCCTGTGTTTGAGACAGCCTACGGGAAAATTGCAATAAATATATGTTACGGGAGACACCACCCTTTGAACTGGTTAGCTTTCGGCTTCAATGGTGCTGAGATTGTTTTCAACCCTTCTGCGACTGTTGGTGAACTCAGTGAACCAATGTGGCCTATTGAGGCCCGAAATGCTGCAATAGCAAATAGTTATTTTGTTGGATCGATCAATCGTGTCGGGACCGAGATTTTCCCCAATCCATTCACGTCAGGTGATGGGAAACCACAGCATACAGATTTTGGTCACTTTTACGGGTCCAGCCATTTCTCTGCACCAGATGCCTCTTGCACTCCCTCACTCTCCCGTTTTCGGGATGGTTTATTAATCTCCGACATGGATCTTAACTTGTGTAGGCAGCTGAAAGATAAATGGGGATTTCGAATGACTGCTCGGTACGAGTTGTATGCTGAAATGCTTGCTCGGTATCTGAAGCCTGACTTTGAGCCTCAAGTCATTTCTGATCCCTTGTTACACAAGAAGGCTTCTTGA

Protein sequence

MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLALRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS
Homology
BLAST of Lcy08g000620 vs. ExPASy Swiss-Prot
Match: Q8H183 (Beta-ureidopropionase OS=Arabidopsis thaliana OX=3702 GN=PYD3 PE=1 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 6.2e-206
Identity = 329/393 (83.72%), Postives = 366/393 (93.13%), Query Frame = 0

Query: 25  SVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAF 84
           S+CGYDSLHQLL+ NLKP+++QEV+RLLLG NCG+ L+ I L E  K+LS KHDFDLQA 
Sbjct: 16  SICGYDSLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAA 75

Query: 85  RFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCL 144
            F ADKE +R PR+VRVGLIQNSIALPTTAPFSDQ R I DK+KPIIDAAG +GVNILCL
Sbjct: 76  SFSADKEQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCL 135

Query: 145 QEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLALRYNMVIVSSILERDVNHGETLWN 204
           QEAW MPFAFCTRE+RWCEFAEP+DGEST+FLQ+LA +YNMVIVS ILERD++HGE LWN
Sbjct: 136 QEAWTMPFAFCTRERRWCEFAEPVDGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWN 195

Query: 205 TAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHP 264
           TAV+IGN+GNIIGKHRKNHIPRVGDFNESTYYMEG+TGHPVFET +GKIA+NICYGRHHP
Sbjct: 196 TAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHP 255

Query: 265 LNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFT 324
           LNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTE+FPNPFT
Sbjct: 256 LNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEVFPNPFT 315

Query: 325 SGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMT 384
           SGDGKPQH DFGHFYGSSHFSAPDASCTPSLSR++DGLLISDMDLNLCRQ KDKWGFRMT
Sbjct: 316 SGDGKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMDLNLCRQYKDKWGFRMT 375

Query: 385 ARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           ARYE+YA++LA+Y+KPDF+PQV+SDPLLHK ++
Sbjct: 376 ARYEVYADLLAKYIKPDFKPQVVSDPLLHKNST 408

BLAST of Lcy08g000620 vs. ExPASy Swiss-Prot
Match: Q964D8 (Beta-ureidopropionase OS=Dictyostelium discoideum OX=44689 GN=pyd3 PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.7e-145
Identity = 245/384 (63.80%), Postives = 300/384 (78.12%), Query Frame = 0

Query: 29  YDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAFRFHA 88
           ++S+   L + +  +   EV R+L G N G  ++++ + +    L+ K++F++ A +  A
Sbjct: 5   FESVQATLEKYIPAEELSEVKRILYGYNRGHHVKSLPICQEALDLANKNNFEIVASKVEA 64

Query: 89  DKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAW 148
           D E +R+PRIVR+G+IQNSI   TTAP  DQ  AI  K++ +IDAAGA GVN+LCLQE W
Sbjct: 65  DPEQLRKPRIVRLGIIQNSIGAETTAPIQDQYLAIEAKIEKMIDAAGAMGVNVLCLQETW 124

Query: 149 MMPFAFCTREK-RWCEFAEPID-GESTQFLQDLALRYNMVIVSSILERDVNHGETLWNTA 208
            MPFAFCTREK  W EFAE    G+S +F+Q +A +YNMVI+S +LERD  H  T+ NTA
Sbjct: 125 HMPFAFCTREKYPWVEFAESASTGQSIKFIQRMARKYNMVIISPMLERDDVHASTIHNTA 184

Query: 209 VVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHPLN 268
           VV+GN+GNIIGK RKNHIPR GDFNESTYYME   GHPVFET YGKIAINICYGRHH LN
Sbjct: 185 VVVGNNGNIIGKSRKNHIPRTGDFNESTYYMESTLGHPVFETIYGKIAINICYGRHHNLN 244

Query: 269 WLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFTSG 328
           WLA+G NGAEIVFNPSATVGELSEPMW +EARNAA+ N+YFVGSINRVGTE FPN FTSG
Sbjct: 245 WLAYGLNGAEIVFNPSATVGELSEPMWGVEARNAAMTNNYFVGSINRVGTEHFPNEFTSG 304

Query: 329 DGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMTAR 388
           +GKP H DFGHFYGSS+FS+PD  CTPSLSR  DGL IS++DLNLC+Q+KDKW F+MTAR
Sbjct: 305 NGKPAHKDFGHFYGSSYFSSPDNCCTPSLSRVSDGLNISEVDLNLCQQVKDKWNFQMTAR 364

Query: 389 YELYAEMLARYLKPDFEPQVISDP 411
           YELYA+ L  Y+ P+++P +I DP
Sbjct: 365 YELYAKFLTDYINPNYQPNIIKDP 388

BLAST of Lcy08g000620 vs. ExPASy Swiss-Prot
Match: Q9UBR1 (Beta-ureidopropionase OS=Homo sapiens OX=9606 GN=UPB1 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 1.0e-131
Identity = 225/383 (58.75%), Postives = 281/383 (73.37%), Query Frame = 0

Query: 29  YDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAFRFHA 88
           + SL + L ++L     QEV R+L     GK L+ + L       + + DF+LQ + F A
Sbjct: 6   WKSLEECLEKHLPLPDLQEVKRVLY----GKELRKLDLPREAFEAASREDFELQGYAFEA 65

Query: 89  DKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAW 148
            +E +R PRIV VGL+QN I LP  AP ++Q  A+  ++K I++ A   GVNI+C QEAW
Sbjct: 66  AEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAW 125

Query: 149 MMPFAFCTREK-RWCEFAEPI-DGESTQFLQDLALRYNMVIVSSILERDVNHGETLWNTA 208
            MPFAFCTREK  W EFAE   DG +T+F Q LA  ++MV+VS ILERD  HG+ LWNTA
Sbjct: 126 TMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTA 185

Query: 209 VVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHPLN 268
           VVI N G ++GK RKNHIPRVGDFNESTYYMEGN GHPVF+T +G+IA+NICYGRHHPLN
Sbjct: 186 VVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVNICYGRHHPLN 245

Query: 269 WLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFTSG 328
           WL +  NGAEI+FNPSAT+G LSE +WPIEARNAAIAN  F  +INRVGTE FPN FTSG
Sbjct: 246 WLMYSINGAEIIFNPSATIGALSESLWPIEARNAAIANHCFTCAINRVGTEHFPNEFTSG 305

Query: 329 DGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMTAR 388
           DGK  H DFG+FYGSS+ +APD+S TP LSR RDGLL++ +DLNLC+Q+ D W F+MT R
Sbjct: 306 DGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRSRDGLLVAKLDLNLCQQVNDVWNFKMTGR 365

Query: 389 YELYAEMLARYLKPDFEPQVISD 410
           YE+YA  LA  +K ++ P ++ +
Sbjct: 366 YEMYARELAEAVKSNYSPTIVKE 384

BLAST of Lcy08g000620 vs. ExPASy Swiss-Prot
Match: Q5RBM6 (Beta-ureidopropionase OS=Pongo abelii OX=9601 GN=UPB1 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 3.2e-130
Identity = 223/383 (58.22%), Postives = 279/383 (72.85%), Query Frame = 0

Query: 29  YDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAFRFHA 88
           + SL + L ++L     QEV R+L     GK L+ + L       + + DF+LQ + F A
Sbjct: 6   WKSLEECLEKHLPLPDLQEVKRVLY----GKELRKLDLPREAFEAASREDFELQGYAFEA 65

Query: 89  DKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAW 148
            +E +R PRIV VGL+QN I LP  AP ++Q  A+  ++K I++ A   GVNI+C QEAW
Sbjct: 66  AEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAW 125

Query: 149 MMPFAFCTREK-RWCEFAEPI-DGESTQFLQDLALRYNMVIVSSILERDVNHGETLWNTA 208
            MPFAFCTREK  W EFAE   DG +T+F Q LA  ++MV+VS ILERD  HG+ LWNTA
Sbjct: 126 TMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTA 185

Query: 209 VVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHPLN 268
           VVI N G ++GK RKNHIPRVGDFNESTYYMEGN GHPVF+T +G+IA+NICYGRHHPLN
Sbjct: 186 VVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVNICYGRHHPLN 245

Query: 269 WLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFTSG 328
           WL +  NGAEI+FNPSAT+G LSE +W IEARNAAIAN  F  +INRVGTE FPN FTSG
Sbjct: 246 WLMYSINGAEIIFNPSATIGALSESLWSIEARNAAIANHCFTCAINRVGTEHFPNEFTSG 305

Query: 329 DGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMTAR 388
           DGK  H DFG+FYGSS+ +APD S TP LSR +DGLL++ +DLNLC+Q+ D W F+MT R
Sbjct: 306 DGKKAHQDFGYFYGSSYVAAPDGSRTPGLSRSQDGLLVAKLDLNLCQQVNDVWNFKMTGR 365

Query: 389 YELYAEMLARYLKPDFEPQVISD 410
           YE+YA  LA  +K ++ P ++ +
Sbjct: 366 YEMYARELAEAVKSNYSPTIVKE 384

BLAST of Lcy08g000620 vs. ExPASy Swiss-Prot
Match: Q03248 (Beta-ureidopropionase OS=Rattus norvegicus OX=10116 GN=Upb1 PE=1 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.8e-128
Identity = 220/386 (56.99%), Postives = 283/386 (73.32%), Query Frame = 0

Query: 29  YDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAFRFHA 88
           + SL Q L ++L P    +V R+L     GK  + + L       + + +F+L+ + F A
Sbjct: 6   WQSLEQCLEKHLPPDDLSQVKRILY----GKQTRNLDLPRKALEAASERNFELKGYAFGA 65

Query: 89  DKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAW 148
            KE  R P+IVRVGL+QN I LPT+AP ++Q  A+  +++ I + A   GVNI+C QEAW
Sbjct: 66  AKEQQRCPQIVRVGLVQNRIPLPTSAPVAEQVSALHKRIEEIAEVAAMCGVNIICFQEAW 125

Query: 149 MMPFAFCTREK-RWCEFAEPI-DGESTQFLQDLALRYNMVIVSSILERDVNHGETLWNTA 208
            MPFAFCTREK  W EFAE   DG +T+F Q LA ++NMV++S ILERD +HG  LWNTA
Sbjct: 126 NMPFAFCTREKLPWTEFAESAEDGLTTRFCQKLAKKHNMVVISPILERDRDHGGVLWNTA 185

Query: 209 VVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHPLN 268
           VVI N G ++GK RKNHIPRVGDFNESTYYMEGN GHPVF+T +G+IA+NICYGRHHPLN
Sbjct: 186 VVISNSGLVMGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVNICYGRHHPLN 245

Query: 269 WLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFTSG 328
           WL +  NGAEI+FNPSAT+GELSE MWPIEARNAAIAN  F  ++NRVG E +PN FTSG
Sbjct: 246 WLMYSVNGAEIIFNPSATIGELSESMWPIEARNAAIANHCFTCALNRVGQEHYPNEFTSG 305

Query: 329 DGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMTAR 388
           DGK  H D G+FYGSS+ +APD S TP LSR +DGLL+++++LNLC+Q+ D W F+MT R
Sbjct: 306 DGKKAHHDLGYFYGSSYVAAPDGSRTPGLSRNQDGLLVTELNLNLCQQINDFWTFKMTGR 365

Query: 389 YELYAEMLARYLKPDFEPQVISDPLL 413
            E+YA  LA  +KP++ P ++ + L+
Sbjct: 366 LEMYARELAEAVKPNYSPNIVKEDLV 387

BLAST of Lcy08g000620 vs. ExPASy TrEMBL
Match: A0A6J1I4J4 (beta-ureidopropionase OS=Cucurbita maxima OX=3661 GN=LOC111470599 PE=4 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 4.3e-234
Identity = 397/417 (95.20%), Postives = 404/417 (96.88%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK S TDA NGE NDGK AIN ASVCGYDSLHQLLA NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISNTDAVNGEVNDGKDAINDASVCGYDSLHQLLAANLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQT+SLAEPVKSLSLKHDFDLQAFRF ADKESVR PRIVRVGLIQNSIALPTTAPFSDQK
Sbjct: 61  LQTVSLAEPVKSLSLKHDFDLQAFRFIADKESVRVPRIVRVGLIQNSIALPTTAPFSDQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAIL+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA
Sbjct: 121 RAILEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           L+YNMVIVSSILERDV+HGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LKYNMVIVSSILERDVSHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKIAINICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTE FPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSR RD
Sbjct: 301 AIANSYFVGSINRVGTETFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRSRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYE+YAE LARYLKP+FEPQVISDPLLHKKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYEMYAETLARYLKPEFEPQVISDPLLHKKAS 417

BLAST of Lcy08g000620 vs. ExPASy TrEMBL
Match: A0A6J1GL16 (beta-ureidopropionase OS=Cucurbita moschata OX=3662 GN=LOC111455342 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.0e-232
Identity = 394/417 (94.48%), Postives = 402/417 (96.40%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK S TDA NGE NDGK A N ASVCGYDSLHQLLA NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISNTDAVNGELNDGKDATNDASVCGYDSLHQLLAANLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQT+ LAEPVKSLSLKHDFDLQAFRF ADKESVR PRIVRVGLIQNSIALPTT+PFSDQK
Sbjct: 61  LQTVPLAEPVKSLSLKHDFDLQAFRFIADKESVRVPRIVRVGLIQNSIALPTTSPFSDQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAIL+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA
Sbjct: 121 RAILEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           L+YNMVIVSSILERDV+HGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LKYNMVIVSSILERDVSHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKIAINICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTE FPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSR RD
Sbjct: 301 AIANSYFVGSINRVGTETFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRSRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYE+YAE LARYLKP+FEPQVISDPLLHKKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYEMYAETLARYLKPEFEPQVISDPLLHKKAS 417

BLAST of Lcy08g000620 vs. ExPASy TrEMBL
Match: A0A5A7SL45 (Beta-ureidopropionase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002100 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.4e-231
Identity = 389/412 (94.42%), Postives = 400/412 (97.09%), Query Frame = 0

Query: 4   TSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQT 63
           TS TDAAN E NDGK A N+ SVCGYDSLHQLLA NLKP IFQEVSRLLLGLNCGKPLQT
Sbjct: 6   TSITDAANRELNDGKDATNNGSVCGYDSLHQLLAANLKPHIFQEVSRLLLGLNCGKPLQT 65

Query: 64  ISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAI 123
           +SLAEPV SLSL+HDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPF DQKRAI
Sbjct: 66  LSLAEPVNSLSLEHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFLDQKRAI 125

Query: 124 LDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLALRY 183
           L+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA+RY
Sbjct: 126 LEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLAIRY 185

Query: 184 NMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGH 243
           NMVIVSSILERDV+HG+TLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGH
Sbjct: 186 NMVIVSSILERDVSHGDTLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGH 245

Query: 244 PVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIA 303
           PVFETAYGKI INICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNAAIA
Sbjct: 246 PVFETAYGKIGINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAIA 305

Query: 304 NSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLL 363
           NSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLL
Sbjct: 306 NSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLL 365

Query: 364 ISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKK 416
           ISDMDLNLCRQLKDKWGFRMTARYELYA++LA+YLKPDFEPQVISDPLLHKK
Sbjct: 366 ISDMDLNLCRQLKDKWGFRMTARYELYAKLLAQYLKPDFEPQVISDPLLHKK 417

BLAST of Lcy08g000620 vs. ExPASy TrEMBL
Match: A0A1S3C0D2 (beta-ureidopropionase OS=Cucumis melo OX=3656 GN=LOC103495390 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.4e-231
Identity = 389/412 (94.42%), Postives = 400/412 (97.09%), Query Frame = 0

Query: 4   TSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQT 63
           TS TDAAN E NDGK A N+ SVCGYDSLHQLLA NLKP IFQEVSRLLLGLNCGKPLQT
Sbjct: 6   TSITDAANRELNDGKDATNNGSVCGYDSLHQLLAANLKPHIFQEVSRLLLGLNCGKPLQT 65

Query: 64  ISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAI 123
           +SLAEPV SLSL+HDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPF DQKRAI
Sbjct: 66  LSLAEPVNSLSLEHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFLDQKRAI 125

Query: 124 LDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLALRY 183
           L+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA+RY
Sbjct: 126 LEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLAIRY 185

Query: 184 NMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGH 243
           NMVIVSSILERDV+HG+TLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGH
Sbjct: 186 NMVIVSSILERDVSHGDTLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGH 245

Query: 244 PVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIA 303
           PVFETAYGKI INICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNAAIA
Sbjct: 246 PVFETAYGKIGINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAIA 305

Query: 304 NSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLL 363
           NSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLL
Sbjct: 306 NSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLL 365

Query: 364 ISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKK 416
           ISDMDLNLCRQLKDKWGFRMTARYELYA++LA+YLKPDFEPQVISDPLLHKK
Sbjct: 366 ISDMDLNLCRQLKDKWGFRMTARYELYAKLLAQYLKPDFEPQVISDPLLHKK 417

BLAST of Lcy08g000620 vs. ExPASy TrEMBL
Match: A0A6J1JI94 (beta-ureidopropionase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486031 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 5.8e-231
Identity = 391/417 (93.76%), Postives = 404/417 (96.88%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK SQ  AAN E NDGK AIN+ SVCGYDSLHQLLA+NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISQAGAANHELNDGKDAINNVSVCGYDSLHQLLAQNLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQTISLAEPVKSLSLKHDFDLQAF FHADKESVREPRIVRVGLIQNSIALPTTAPFS+QK
Sbjct: 61  LQTISLAEPVKSLSLKHDFDLQAFCFHADKESVREPRIVRVGLIQNSIALPTTAPFSNQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           +AI +KVKPII+AAGASGVNILCLQEAWMMPFAFCTREK WCEFAEP+DGESTQFLQDLA
Sbjct: 121 KAIFEKVKPIIEAAGASGVNILCLQEAWMMPFAFCTREKGWCEFAEPVDGESTQFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
            RYNMVIVSSILERDVNHGETLWNTA+VIGN+GNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 SRYNMVIVSSILERDVNHGETLWNTALVIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKIAINICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIAN YFVGSINRVGTEIFPNPFTSG+G+PQHTDFGHFYGSSHFSAPDASCTPSLSRFRD
Sbjct: 301 AIANIYFVGSINRVGTEIFPNPFTSGNGRPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKP+FEPQVISDPLL+KKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPEFEPQVISDPLLNKKAS 417

BLAST of Lcy08g000620 vs. NCBI nr
Match: XP_022971926.1 (beta-ureidopropionase [Cucurbita maxima])

HSP 1 Score: 820.1 bits (2117), Expect = 8.8e-234
Identity = 397/417 (95.20%), Postives = 404/417 (96.88%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK S TDA NGE NDGK AIN ASVCGYDSLHQLLA NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISNTDAVNGEVNDGKDAINDASVCGYDSLHQLLAANLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQT+SLAEPVKSLSLKHDFDLQAFRF ADKESVR PRIVRVGLIQNSIALPTTAPFSDQK
Sbjct: 61  LQTVSLAEPVKSLSLKHDFDLQAFRFIADKESVRVPRIVRVGLIQNSIALPTTAPFSDQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAIL+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA
Sbjct: 121 RAILEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           L+YNMVIVSSILERDV+HGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LKYNMVIVSSILERDVSHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKIAINICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTE FPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSR RD
Sbjct: 301 AIANSYFVGSINRVGTETFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRSRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYE+YAE LARYLKP+FEPQVISDPLLHKKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYEMYAETLARYLKPEFEPQVISDPLLHKKAS 417

BLAST of Lcy08g000620 vs. NCBI nr
Match: XP_038889066.1 (beta-ureidopropionase [Benincasa hispida])

HSP 1 Score: 816.2 bits (2107), Expect = 1.3e-232
Identity = 392/417 (94.00%), Postives = 404/417 (96.88%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEKT  T A +GE NDGK AIN+ SVCGYDSLH+LLA NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKTPNTHATDGELNDGKDAINNGSVCGYDSLHRLLAANLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQTISLAEPV SLSL+HDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPF DQ 
Sbjct: 61  LQTISLAEPVNSLSLEHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFLDQN 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAIL+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA
Sbjct: 121 RAILEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           L+YNMVIVSSILERDV+HG+TLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LKYNMVIVSSILERDVSHGDTLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKI INICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIGINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSR+RD
Sbjct: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRYRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYE+YAE+LARYLKPDFEPQVISDPLLHKKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYEVYAEILARYLKPDFEPQVISDPLLHKKAS 417

BLAST of Lcy08g000620 vs. NCBI nr
Match: XP_022952736.1 (beta-ureidopropionase [Cucurbita moschata] >KAG6571870.1 Beta-ureidopropionase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011551.1 Beta-ureidopropionase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-232
Identity = 394/417 (94.48%), Postives = 402/417 (96.40%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK S TDA NGE NDGK A N ASVCGYDSLHQLLA NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISNTDAVNGELNDGKDATNDASVCGYDSLHQLLAANLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQT+ LAEPVKSLSLKHDFDLQAFRF ADKESVR PRIVRVGLIQNSIALPTT+PFSDQK
Sbjct: 61  LQTVPLAEPVKSLSLKHDFDLQAFRFIADKESVRVPRIVRVGLIQNSIALPTTSPFSDQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAIL+KVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGEST+FLQDLA
Sbjct: 121 RAILEKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           L+YNMVIVSSILERDV+HGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LKYNMVIVSSILERDVSHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKIAINICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTE FPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSR RD
Sbjct: 301 AIANSYFVGSINRVGTETFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRSRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYE+YAE LARYLKP+FEPQVISDPLLHKKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYEMYAETLARYLKPEFEPQVISDPLLHKKAS 417

BLAST of Lcy08g000620 vs. NCBI nr
Match: KAG6588746.1 (Beta-ureidopropionase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 813.1 bits (2099), Expect = 1.1e-231
Identity = 393/417 (94.24%), Postives = 406/417 (97.36%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK SQ  AAN E NDGK AIN+ SVCGYDSLHQLLA+NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISQACAANHELNDGKDAINNVSVCGYDSLHQLLAQNLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQTISLAEPVKSLSLKHDFDLQAF FHADKESVREPRIVRVGLIQNSIALPTTAPFS+QK
Sbjct: 61  LQTISLAEPVKSLSLKHDFDLQAFCFHADKESVREPRIVRVGLIQNSIALPTTAPFSNQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAI +KVKPII+AAGASGVNILCLQEAWMMPFAFCTREK WCEFAEP+DGEST+FLQDLA
Sbjct: 121 RAIFEKVKPIIEAAGASGVNILCLQEAWMMPFAFCTREKGWCEFAEPVDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           LR+NMVIVSSILERDVNHGETLWNTAVVIGN+GNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LRFNMVIVSSILERDVNHGETLWNTAVVIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETA+GKIAINICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAFGKIAINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTEIFPNPFTSGDG+PQHTDFGHFYGSSHFSAPDASCTPSLSRFRD
Sbjct: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGRPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKP+FEPQVISDPLL+KKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPEFEPQVISDPLLNKKAS 417

BLAST of Lcy08g000620 vs. NCBI nr
Match: XP_023553889.1 (beta-ureidopropionase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 812.8 bits (2098), Expect = 1.4e-231
Identity = 393/417 (94.24%), Postives = 400/417 (95.92%), Query Frame = 0

Query: 1   MEKTSQTDAANGERNDGKHAINSASVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKP 60
           MEK S TDA NGE NDGK A N ASVCGYDSLHQLLA NLKPQIFQEVSRLLLGLNCGKP
Sbjct: 1   MEKISNTDAVNGELNDGKDATNDASVCGYDSLHQLLAANLKPQIFQEVSRLLLGLNCGKP 60

Query: 61  LQTISLAEPVKSLSLKHDFDLQAFRFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQK 120
           LQT+ LAEPVKSLSLKHDFDLQAFRF ADKESVR PRIVRVGLIQNSIALPTTAPFSDQK
Sbjct: 61  LQTVPLAEPVKSLSLKHDFDLQAFRFIADKESVRVPRIVRVGLIQNSIALPTTAPFSDQK 120

Query: 121 RAILDKVKPIIDAAGASGVNILCLQEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLA 180
           RAIL+KVKPIIDAAGASGVNILCLQEAW MPFAFCTREKRWCEFAEPIDGEST+FLQDLA
Sbjct: 121 RAILEKVKPIIDAAGASGVNILCLQEAWTMPFAFCTREKRWCEFAEPIDGESTRFLQDLA 180

Query: 181 LRYNMVIVSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240
           L+YNMVIVSSILERDV+HGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN
Sbjct: 181 LKYNMVIVSSILERDVSHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN 240

Query: 241 TGHPVFETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNA 300
           TGHPVFETAYGKI INICYGRHHPLNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNA
Sbjct: 241 TGHPVFETAYGKIGINICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNA 300

Query: 301 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 360
           AIANSYFVGSINRVGTE FPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSR RD
Sbjct: 301 AIANSYFVGSINRVGTETFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRSRD 360

Query: 361 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           GLLISDMDLNLCRQLKDKWGFRMTARYE+YAE LARYLKP+FEPQVISDPLLHKKAS
Sbjct: 361 GLLISDMDLNLCRQLKDKWGFRMTARYEMYAETLARYLKPEFEPQVISDPLLHKKAS 417

BLAST of Lcy08g000620 vs. TAIR 10
Match: AT5G64370.1 (beta-ureidopropionase )

HSP 1 Score: 718.0 bits (1852), Expect = 4.4e-207
Identity = 329/393 (83.72%), Postives = 366/393 (93.13%), Query Frame = 0

Query: 25  SVCGYDSLHQLLAENLKPQIFQEVSRLLLGLNCGKPLQTISLAEPVKSLSLKHDFDLQAF 84
           S+CGYDSLHQLL+ NLKP+++QEV+RLLLG NCG+ L+ I L E  K+LS KHDFDLQA 
Sbjct: 16  SICGYDSLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAA 75

Query: 85  RFHADKESVREPRIVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCL 144
            F ADKE +R PR+VRVGLIQNSIALPTTAPFSDQ R I DK+KPIIDAAG +GVNILCL
Sbjct: 76  SFSADKEQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCL 135

Query: 145 QEAWMMPFAFCTREKRWCEFAEPIDGESTQFLQDLALRYNMVIVSSILERDVNHGETLWN 204
           QEAW MPFAFCTRE+RWCEFAEP+DGEST+FLQ+LA +YNMVIVS ILERD++HGE LWN
Sbjct: 136 QEAWTMPFAFCTRERRWCEFAEPVDGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWN 195

Query: 205 TAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRHHP 264
           TAV+IGN+GNIIGKHRKNHIPRVGDFNESTYYMEG+TGHPVFET +GKIA+NICYGRHHP
Sbjct: 196 TAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHP 255

Query: 265 LNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNPFT 324
           LNWLAFG NGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTE+FPNPFT
Sbjct: 256 LNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEVFPNPFT 315

Query: 325 SGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDLNLCRQLKDKWGFRMT 384
           SGDGKPQH DFGHFYGSSHFSAPDASCTPSLSR++DGLLISDMDLNLCRQ KDKWGFRMT
Sbjct: 316 SGDGKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMDLNLCRQYKDKWGFRMT 375

Query: 385 ARYELYAEMLARYLKPDFEPQVISDPLLHKKAS 418
           ARYE+YA++LA+Y+KPDF+PQV+SDPLLHK ++
Sbjct: 376 ARYEVYADLLAKYIKPDFKPQVVSDPLLHKNST 408

BLAST of Lcy08g000620 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 100.5 bits (249), Expect = 3.3e-21
Identity = 79/274 (28.83%), Postives = 126/274 (45.99%), Query Frame = 0

Query: 130 IIDAAGASGVNILCLQEAWMMPFAFCTREKR-WCEFAEPIDGEST-QFLQDLALRYNMVI 189
           ++  A A G NI+ +QE +   + FC  ++  + + A+P     T   +Q LA    +VI
Sbjct: 33  LVREAHAKGANIILIQELF-EGYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 92

Query: 190 -VSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVF 249
            VS   E +  H    +N+  +I   G  +G +RK+HIP    + E  Y+  G+TG  VF
Sbjct: 93  PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 152

Query: 250 ETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGEL------SEPMWPIEARNA 309
           +T + KI + IC+ +  P    A    GAEI+F P+A   E       S   W    +  
Sbjct: 153 QTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGH 212

Query: 310 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 369
           A AN   + + NR+G EI      +  G  Q T    FYG+S  + P            +
Sbjct: 213 AGANVVPLVASNRIGKEI----IETEHGPSQIT----FYGTSFIAGPTGEIVAEADDKSE 272

Query: 370 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEML 395
            +L++  DL++ +  +  WG     R +LY  +L
Sbjct: 273 AVLVAQFDLDMIKSKRQSWGVFRDRRPDLYKVLL 293

BLAST of Lcy08g000620 vs. TAIR 10
Match: AT2G27450.2 (nitrilase-like protein 1 )

HSP 1 Score: 100.5 bits (249), Expect = 3.3e-21
Identity = 79/274 (28.83%), Postives = 126/274 (45.99%), Query Frame = 0

Query: 130 IIDAAGASGVNILCLQEAWMMPFAFCTREKR-WCEFAEPIDGEST-QFLQDLALRYNMVI 189
           ++  A A G NI+ +QE +   + FC  ++  + + A+P     T   +Q LA    +VI
Sbjct: 60  LVREAHAKGANIILIQELF-EGYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 119

Query: 190 -VSSILERDVNHGETLWNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVF 249
            VS   E +  H    +N+  +I   G  +G +RK+HIP    + E  Y+  G+TG  VF
Sbjct: 120 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 179

Query: 250 ETAYGKIAINICYGRHHPLNWLAFGFNGAEIVFNPSATVGEL------SEPMWPIEARNA 309
           +T + KI + IC+ +  P    A    GAEI+F P+A   E       S   W    +  
Sbjct: 180 QTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGH 239

Query: 310 AIANSYFVGSINRVGTEIFPNPFTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRD 369
           A AN   + + NR+G EI      +  G  Q T    FYG+S  + P            +
Sbjct: 240 AGANVVPLVASNRIGKEI----IETEHGPSQIT----FYGTSFIAGPTGEIVAEADDKSE 299

Query: 370 GLLISDMDLNLCRQLKDKWGFRMTARYELYAEML 395
            +L++  DL++ +  +  WG     R +LY  +L
Sbjct: 300 AVLVAQFDLDMIKSKRQSWGVFRDRRPDLYKVLL 320

BLAST of Lcy08g000620 vs. TAIR 10
Match: AT3G44320.1 (nitrilase 3 )

HSP 1 Score: 63.5 bits (153), Expect = 4.5e-10
Identity = 83/332 (25.00%), Postives = 129/332 (38.86%), Query Frame = 0

Query: 99  VRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAWM--MPFAF-- 158
           VRV ++Q+S     T        A LDK +  I  A + G  ++   EA++   P  F  
Sbjct: 25  VRVTIVQSSTVYNDTP-------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRF 84

Query: 159 -----CTREKRWCEF------AEPIDGESTQFLQDLALRYNMVIVSSILERDVNHGETLW 218
                   E+   EF      A  + G   + L +LA + N+ +V   +E+D   G TL+
Sbjct: 85  GLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---GYTLY 144

Query: 219 NTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGN-TGHPVFETAYGKIAINICYGRH 278
            TA+     G  +GKHRK     +    E   + +G+ +  PV++T  GKI   IC+   
Sbjct: 145 CTALFFSPQGQFLGKHRKVMPTSL----ERCIWGQGDGSTIPVYDTPIGKIGAAICWENR 204

Query: 279 HPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINRVGTEIFPNP 338
            PL   A    G EI   P+A         W     + A+    FV S ++         
Sbjct: 205 MPLYRTALYAKGIEIYCAPTADY----SLEWQASMIHIAVEGGCFVLSAHQ--------- 264

Query: 339 FTSGDGKPQHTDF-------------GHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDL 398
           F      P+H D+                 G S   +P        +   +GL+ +D+DL
Sbjct: 265 FCKRREFPEHPDYLFNDIVDTKEHDPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDL 317

Query: 399 NLCRQLKDKWGFRMTARYELYAEMLARYLKPD 402
                          AR +LY +++  Y KPD
Sbjct: 325 G------------DIARAKLYFDVVGHYSKPD 317

BLAST of Lcy08g000620 vs. TAIR 10
Match: AT5G22300.1 (nitrilase 4 )

HSP 1 Score: 59.7 bits (143), Expect = 6.5e-09
Identity = 70/291 (24.05%), Postives = 116/291 (39.86%), Query Frame = 0

Query: 98  IVRVGLIQNSIALPTTAPFSDQKRAILDKVKPIIDAAGASGVNILCLQEAWM-------- 157
           IVR  ++Q S     T        A LDK + ++  A  +G  ++   EA++        
Sbjct: 35  IVRATVVQASTVFYDTP-------ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGST 94

Query: 158 MPFAFCTRE-------KRWCEFAEPIDGESTQFLQDLALRYNMVIVSSILERDVNHGETL 217
              A  +R        +++   A  + G   + L  +A +Y + +V  ++ER+   G TL
Sbjct: 95  FELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKVYLVMGVIERE---GYTL 154

Query: 218 WNTAVVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETAYGKIAINICYGRH 277
           + T +   + G  +GKHRK  +P         +     +  PVF+T  GKI   IC+   
Sbjct: 155 YCTVLFFDSQGLFLGKHRK-LMPTA--LERCIWGFGDGSTIPVFDTPIGKIGAAICWENR 214

Query: 278 HPLNWLAFGFNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINR-VGTEIFPN 337
            P    A    G EI   P+A     S   W     + A+    FV S N+    + +P+
Sbjct: 215 MPSLRTAMYAKGIEIYCAPTAD----SRETWLASMTHIALEGGCFVLSANQFCRRKDYPS 274

Query: 338 P---FTSGDGKPQHTDFGHFYGSSHFSAPDASCTPSLSRFRDGLLISDMDL 370
           P     SG  +    D     G S   +P        +   + L+ +D+DL
Sbjct: 275 PPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDL 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1836.2e-20683.72Beta-ureidopropionase OS=Arabidopsis thaliana OX=3702 GN=PYD3 PE=1 SV=1[more]
Q964D82.7e-14563.80Beta-ureidopropionase OS=Dictyostelium discoideum OX=44689 GN=pyd3 PE=1 SV=1[more]
Q9UBR11.0e-13158.75Beta-ureidopropionase OS=Homo sapiens OX=9606 GN=UPB1 PE=1 SV=1[more]
Q5RBM63.2e-13058.22Beta-ureidopropionase OS=Pongo abelii OX=9601 GN=UPB1 PE=2 SV=1[more]
Q032481.8e-12856.99Beta-ureidopropionase OS=Rattus norvegicus OX=10116 GN=Upb1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I4J44.3e-23495.20beta-ureidopropionase OS=Cucurbita maxima OX=3661 GN=LOC111470599 PE=4 SV=1[more]
A0A6J1GL161.0e-23294.48beta-ureidopropionase OS=Cucurbita moschata OX=3662 GN=LOC111455342 PE=4 SV=1[more]
A0A5A7SL454.4e-23194.42Beta-ureidopropionase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13... [more]
A0A1S3C0D24.4e-23194.42beta-ureidopropionase OS=Cucumis melo OX=3656 GN=LOC103495390 PE=4 SV=1[more]
A0A6J1JI945.8e-23193.76beta-ureidopropionase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486031 PE=... [more]
Match NameE-valueIdentityDescription
XP_022971926.18.8e-23495.20beta-ureidopropionase [Cucurbita maxima][more]
XP_038889066.11.3e-23294.00beta-ureidopropionase [Benincasa hispida][more]
XP_022952736.12.2e-23294.48beta-ureidopropionase [Cucurbita moschata] >KAG6571870.1 Beta-ureidopropionase, ... [more]
KAG6588746.11.1e-23194.24Beta-ureidopropionase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023553889.11.4e-23194.24beta-ureidopropionase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G64370.14.4e-20783.72beta-ureidopropionase [more]
AT2G27450.13.3e-2128.83nitrilase-like protein 1 [more]
AT2G27450.23.3e-2128.83nitrilase-like protein 1 [more]
AT3G44320.14.5e-1025.00nitrilase 3 [more]
AT5G22300.16.5e-0924.05nitrilase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 101..376
e-value: 4.8E-52
score: 176.9
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 99..369
score: 51.364071
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 25..410
e-value: 4.3E-120
score: 403.0
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 94..385
NoneNo IPR availablePANTHERPTHR43674:SF11BNAANNG15120D PROTEINcoord: 20..411
NoneNo IPR availablePANTHERPTHR43674NITRILASE C965.09-RELATEDcoord: 20..411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g000620.1Lcy08g000620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033396 beta-alanine biosynthetic process via 3-ureidopropionate
biological_process GO:0006807 nitrogen compound metabolic process
molecular_function GO:0003837 beta-ureidopropionase activity