Lcy07g002160 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g002160
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	dTMP kinase
Location	Chr07: 7606717 .. 7607430 (-)
RNA-Seq Expression	Lcy07g002160
Synteny	Lcy07g002160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAACTAATCAGAACTCTATACTCAGAGGTTGCGGCAGTAACTCTAGAGGAGCCTTGGTGGTTCTTGAAGGTTTGAACCGTTGTGGGAAGACTACACAGTCCAGTAGATTAGTCCAGTACTTAGAAGGACTTGGGCATTCTGCTGAACTATGGCGGTTTCCTGACAGAACTACAAGTGTTGGATAGATGATATCTTCCTATCTTTCAAACAAATCACAGCTGGATGACCACACAATACATCTTCTTTTCAGTGCTAACCGATGGGAGAAGAGGTTTTTCAATTTTTATATTGGGTTTTTGTATGGAACTCAATCAATTGTATTGTTGTTCAGTATTTGCTTTTACAAGTACTTGGGAGATCAGCAGCAGTGCTAAAATTGATGCTATTACTATGGTAAGCTTCATTTCAATTCTGATTCTTCTTCTGTCACAATCTTGTATGCATTTTGATGTTTTTCTGTTGTGTGTATAAAAGGGCATATATAGACTGGTTTGCTTTGACCTTGCCTTGAAGTTTGTAATTATAATGTTGAGTTCTATAGCATTTGTGCTTGACTTCTTGAGTTTCAATTTCAAAGAGCAGAAGTCTGATAGCTTAAAGGATTTAAATGCAGCACAGTGAATTAATAGACACAACATCCGATGCTAGAAGGGTGGGTGGGAAGCAAGGACTTGCTACCTTCAAATTTGTGACTTCCAATGCCCATTTAT

mRNA sequence

ATGGGAACTAATCAGAACTCTATACTCAGAGGTTGCGGCAGTAACTCTAGAGGAGCCTTGGTGGTTCTTGAAGGTTTGAACCGTTGTGGGAAGACTACACAGTCCAGTAGATTAGTCCAGTACTTAGAAGGACTTGGGCATTCTGCTGAACTATGGCGGTTTCCTGACAGAACTACAATATTTGCTTTTACAAGTACTTGGGAGATCAGCAGCAGTGCTAAAATTGATGCTATTACTATGCACAGTGAATTAATAGACACAACATCCGATGCTAGAAGGGTGGGTGGGAAGCAAGGACTTGCTACCTTCAAATTTGTGACTTCCAATGCCCATTTAT

Coding sequence (CDS)

ATGGGAACTAATCAGAACTCTATACTCAGAGGTTGCGGCAGTAACTCTAGAGGAGCCTTGGTGGTTCTTGAAGGTTTGAACCGTTGTGGGAAGACTACACAGTCCAGTAGATTAGTCCAGTACTTAGAAGGACTTGGGCATTCTGCTGAACTATGGCGGTTTCCTGACAGAACTACAATATTTGCTTTTACAAGTACTTGGGAGATCAGCAGCAGTGCTAAAATTGATGCTATTACTATGCACAGTGAATTAATAGACACAACATCCGATGCTAGAAGGGTGGGTGGGAAGCAAGGACTTGCTACCTTCAAATTTGTGACTTCCAATGCCCATTTAT

Protein sequence

MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTIFAFTSTWEISSSAKIDAITMHSELIDTTSDARRVGGKQGLATFKFVTSNAHL

Homology

[Close]

BLAST of Lcy07g002160 vs. ExPASy Swiss-Prot
Match: Q0WW55 (Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 4.8e-12
Identity = 33/65 (50.77%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17  RGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTIFAFTSTWEISSSAKID 76
           RGAL+VLEGL+R GK+TQ ++L+ +L GLGH  ELWRFPDR T      +  +S+ +++D
Sbjct: 58  RGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLD 117

Query: 77  AITMH 82
             T+H
Sbjct: 118 DHTIH 122

[Close]

BLAST of Lcy07g002160 vs. ExPASy Swiss-Prot
Match: P23919 (Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4)

HSP 1 Score: 58.2 bits (139), Expect = 7.1e-08
Identity = 27/43 (62.79%), Postives = 35/43 (81.40%), Query Frame = 0


Query: 17 RGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 60
          RGAL+VLEG++R GK+TQS +LV+ L   GH AEL RFP+R+T
Sbjct: 5  RGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERST 47

[Close]

BLAST of Lcy07g002160 vs. ExPASy Swiss-Prot
Match: P97930 (Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 3.5e-07
Identity = 27/46 (58.70%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 14 SNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 60
          ++ RGAL+VLEG++R GKTTQ  +LV  L   GH AEL RFP+R+T
Sbjct: 2  ASRRGALIVLEGVDRAGKTTQGLKLVTALCASGHRAELLRFPERST 47

[Close]

BLAST of Lcy07g002160 vs. ExPASy Swiss-Prot
Match: P36590 (Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tmp1 PE=2 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 2.5e-05
Identity = 24/44 (54.55%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 16 SRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 60
          +RG L+V+EGL+R GK+TQ   LV  L      AEL++FPDRTT
Sbjct: 5  NRGRLIVIEGLDRSGKSTQCQLLVDKLISQHEKAELFKFPDRTT 48

[Close]

BLAST of Lcy07g002160 vs. ExPASy TrEMBL
Match: A0A6J1KDZ8 (dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.5e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1  MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
          MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 1  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 60

Query: 61 FAFTSTWEISSSAKIDAITMH 82
               +  +S+ +++D  T+H
Sbjct: 61 VGQMISSYLSNKSQLDDHTIH 81

[Close]

BLAST of Lcy07g002160 vs. ExPASy TrEMBL
Match: A0A6J1KGD6 (dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.5e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1   MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
           MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 38  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 97

Query: 61  FAFTSTWEISSSAKIDAITMH 82
                +  +S+ +++D  T+H
Sbjct: 98  VGQMISSYLSNKSQLDDHTIH 118

[Close]

BLAST of Lcy07g002160 vs. ExPASy TrEMBL
Match: A0A6J1G9Z9 (dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.5e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1   MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
           MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 38  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 97

Query: 61  FAFTSTWEISSSAKIDAITMH 82
                +  +S+ +++D  T+H
Sbjct: 98  VGQMISSYLSNKSQLDDHTIH 118

[Close]

BLAST of Lcy07g002160 vs. ExPASy TrEMBL
Match: A0A6J1G9C4 (dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.5e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1  MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
          MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 1  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 60

Query: 61 FAFTSTWEISSSAKIDAITMH 82
               +  +S+ +++D  T+H
Sbjct: 61 VGQMISSYLSNKSQLDDHTIH 81

[Close]

BLAST of Lcy07g002160 vs. ExPASy TrEMBL
Match: A0A6J1DF06 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111019882 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 7.4e-24
Identity = 59/81 (72.84%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 1  MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
          MGTNQNS+LRGC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLG SAELWRFPDRTT 
Sbjct: 1  MGTNQNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGFSAELWRFPDRTTS 60

Query: 61 FAFTSTWEISSSAKIDAITMH 82
               +  +S+ +++D  T+H
Sbjct: 61 VGQMISSYLSNKSQLDDRTIH 81

[Close]

BLAST of Lcy07g002160 vs. NCBI nr
Match: KAG6606822.1 (Thymidylate kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1   MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
           MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 562 MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 621

Query: 61  FAFTSTWEISSSAKIDAITMH 82
                +  +S+ +++D  T+H
Sbjct: 622 VGQMISSYLSNKSQLDDHTIH 642

[Close]

BLAST of Lcy07g002160 vs. NCBI nr
Match: XP_022998308.1 (thymidylate kinase isoform X1 [Cucurbita maxima])

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1   MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
           MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 38  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 97

Query: 61  FAFTSTWEISSSAKIDAITMH 82
                +  +S+ +++D  T+H
Sbjct: 98  VGQMISSYLSNKSQLDDHTIH 118

[Close]

BLAST of Lcy07g002160 vs. NCBI nr
Match: XP_023523991.1 (thymidylate kinase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1   MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
           MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 38  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 97

Query: 61  FAFTSTWEISSSAKIDAITMH 82
                +  +S+ +++D  T+H
Sbjct: 98  VGQMISSYLSNKSQLDDHTIH 118

[Close]

BLAST of Lcy07g002160 vs. NCBI nr
Match: XP_022998309.1 (thymidylate kinase isoform X2 [Cucurbita maxima])

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1  MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
          MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 1  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 60

Query: 61 FAFTSTWEISSSAKIDAITMH 82
               +  +S+ +++D  T+H
Sbjct: 61 VGQMISSYLSNKSQLDDHTIH 81

[Close]

BLAST of Lcy07g002160 vs. NCBI nr
Match: XP_022948491.1 (thymidylate kinase isoform X2 [Cucurbita moschata])

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-24
Identity = 60/81 (74.07%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 1  MGTNQNSILRGCGSNSRGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTI 60
          MGTNQNSIL+GC SNSRGALVVLEGL+RCGKTTQSSRLVQYLEGLGHSAELWRFPDRTT 
Sbjct: 1  MGTNQNSILQGCNSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 60

Query: 61 FAFTSTWEISSSAKIDAITMH 82
               +  +S+ +++D  T+H
Sbjct: 61 VGQMISSYLSNKSQLDDHTIH 81

[Close]

BLAST of Lcy07g002160 vs. TAIR 10
Match: AT5G59440.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 3.4e-13
Identity = 33/65 (50.77%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17 RGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTIFAFTSTWEISSSAKID 76
          RGAL+VLEGL+R GK+TQ ++L+ +L GLGH  ELWRFPDR T      +  +S+ +++D
Sbjct: 19 RGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLD 78

Query: 77 AITMH 82
            T+H
Sbjct: 79 DHTIH 83

[Close]

BLAST of Lcy07g002160 vs. TAIR 10
Match: AT5G59440.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 3.4e-13
Identity = 33/65 (50.77%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17  RGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTIFAFTSTWEISSSAKID 76
           RGAL+VLEGL+R GK+TQ ++L+ +L GLGH  ELWRFPDR T      +  +S+ +++D
Sbjct: 58  RGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLD 117

Query: 77  AITMH 82
             T+H
Sbjct: 118 DHTIH 122

[Close]

BLAST of Lcy07g002160 vs. TAIR 10
Match: AT5G59440.3 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 3.4e-13
Identity = 33/65 (50.77%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17  RGALVVLEGLNRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTIFAFTSTWEISSSAKID 76
           RGAL+VLEGL+R GK+TQ ++L+ +L GLGH  ELWRFPDR T      +  +S+ +++D
Sbjct: 66  RGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLD 125

Query: 77  AITMH 82
             T+H
Sbjct: 126 DHTIH 130

The following BLAST results are available for this feature:

BLAST of Lcy07g002160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WW55	4.8e-12	50.77	Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1	[more]
P23919	7.1e-08	62.79	Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4	[more]
P97930	3.5e-07	58.70	Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2	[more]
P36590	2.5e-05	54.55	Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284...	[more]

BLAST of Lcy07g002160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KDZ8	1.5e-24	74.07	dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1	[more]
A0A6J1KGD6	1.5e-24	74.07	dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1	[more]
A0A6J1G9Z9	1.5e-24	74.07	dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1	[more]
A0A6J1G9C4	1.5e-24	74.07	dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1	[more]
A0A6J1DF06	7.4e-24	72.84	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111019882 PE=3 SV=1	[more]

BLAST of Lcy07g002160 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606822.1	3.1e-24	74.07	Thymidylate kinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022998308.1	3.1e-24	74.07	thymidylate kinase isoform X1 [Cucurbita maxima]	[more]
XP_023523991.1	3.1e-24	74.07	thymidylate kinase isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022998309.1	3.1e-24	74.07	thymidylate kinase isoform X2 [Cucurbita maxima]	[more]
XP_022948491.1	3.1e-24	74.07	thymidylate kinase isoform X2 [Cucurbita moschata]	[more]

BLAST of Lcy07g002160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59440.2	3.4e-13	50.77	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.1	3.4e-13	50.77	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.3	3.4e-13	50.77	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 13..93 e-value: 6.3E-12 score: 47.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 17..60
IPR039430	Thymidylate kinase-like domain	PFAM	PF02223	Thymidylate_kin	coord: 23..62 e-value: 1.2E-5 score: 25.0
None	No IPR available	PANTHER	PTHR10344	THYMIDYLATE KINASE	coord: 16..60
None	No IPR available	PANTHER	PTHR10344:SF1	THYMIDYLATE KINASE	coord: 16..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g002160.1	Lcy07g002160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006233	dTDP biosynthetic process
biological_process	GO:0046940	nucleoside monophosphate phosphorylation
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004798	thymidylate kinase activity