Lcy06g022740 (gene) Sponge gourd (P93075) v1

BLAST of Lcy06g022740 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 6.7e-175
Identity = 325/486 (66.87%), Postives = 383/486 (78.81%), Query Frame = 0

Query: 116 EESGSSSDLLTSEATGCEGRSQDEESSSSSSTLMGWPVHQRAAEEGNFDGRELE-RQVSS 175
           EE G  S   +SE T  E   ++E+S S +      PV  R + + N DG + + R  S 
Sbjct: 12  EEKGRESSCCSSETTRQE---EEEQSPSCTEDFTASPVSSRWSVK-NIDGEKKKIRSDSR 71

Query: 176 VSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKA 235
           VSE EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLCATLFGQLWRLEPL  E+K 
Sbjct: 72  VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEKKE 131

Query: 236 MWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDL 295
           MWRREMEWLLCVS+HIVE+ P WQTFPDG+KLEIMTCRPRSDL VNLPALRKLD+MLL++
Sbjct: 132 MWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLLEI 191

Query: 296 LDSFVDSEFWYVDQGILAAE--ADGSSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQL 355
           LDSF ++EFWYVDQGI+A E  ADGSSSFRK  +RQEDKWWLP+PRV  GGL E SR+QL
Sbjct: 192 LDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRKQL 251

Query: 356 QHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVC 415
           QHKRDCTNQILKAAMAINS+TLADM++P SYLE+LP+ GR+ LG+ IYRYISS++FSP C
Sbjct: 252 QHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQFSPEC 311

Query: 416 LLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDAD 475
           LL+CLD+SSEHQAIEIANRVE+++Y W K T SKP       + +K+SWEM+KEL++DAD
Sbjct: 312 LLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPA------TNTKTSWEMVKELMVDAD 371

Query: 476 KTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVT 535
           K +L A+RAESLL  LKQ+FP LPQT+LDMSKIQ+NKD+GK+ILESYSRVLESLAFNIV 
Sbjct: 372 KLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVA 431

Query: 536 RIDDLLYVDDLTKH-SDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAVG 595
           RIDDLL+VDDLT+H SDQIP  + LG    N+ N    S+    S Y+     PS   + 
Sbjct: 432 RIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTTPSYSPSKQELR 481

Query: 596 TRSPIP 598
           +   +P
Sbjct: 492 SSITVP 481

BLAST of Lcy06g022740 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.9e-153
Identity = 286/509 (56.19%), Postives = 370/509 (72.69%), Query Frame = 0

Query: 111 EKSGEEESGSSSDLLT--SEATGCEGRSQDEESSSS---------SSTLMGWPV-----H 170
           EK     + S  D+LT   E +   G S +  S+++          S ++GWP+      
Sbjct: 12  EKKRSNLTPSMEDILTESKERSSTSGASYESSSTTTVASSSPPPPPSQILGWPIRKASFR 71

Query: 171 QRAAEEGNFDGREL--------ERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALA 230
           + + E  NFD ++L        + +  + ++ EMMKERF+KLLLGEDMSG G GVCTALA
Sbjct: 72  KNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALA 131

Query: 231 ISNAITNLCATLFGQLWRLEPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKL 290
           ISNAITNLCAT+FGQLWRLEPL+ E+K MWRREMEW+L VS+HIVEL P  QT+PDG+K 
Sbjct: 132 ISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKF 191

Query: 291 EIMTCRPRSDLCVNLPALRKLDHMLLDLLDSFVDSEFWYVDQGILAAEADGSSSFRKVLE 350
           E+MTCRPR DL +NLPALRKLD+MLLD+L SF  +EFWYVDQGI+A+E DGS+SFR+ ++
Sbjct: 192 EVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQ 251

Query: 351 RQEDKWWLPIPRVPSGGLSEPSRRQLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEA 410
           RQE+KWWLP+PR+   GL+E +R +L HKR+C  QILKAAMAINS+ L +MDVP SYLE 
Sbjct: 252 RQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLET 311

Query: 411 LPKSGRASLGEAIYRYISSEEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSK 470
           LPK+GR+ LG+ IYRY++S++FS   LL+CLD+SSEH A++IANRVEA++Y WR+   +K
Sbjct: 312 LPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTK 371

Query: 471 -PTNHNSSRSTSKSSWEMLKELVIDADKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKI 530
              N+N+S +T K +WEM+KEL+   DK  L  ER+E+LL CLKQ+FP+L QTSLD+SKI
Sbjct: 372 LGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKI 431

Query: 531 QFNKDVGKAILESYSRVLESLAFNIVTRIDDLLYVDDLTKHSD-----QIPGISQLGIVA 589
           Q+NKD+GK+ILESYSR LESLA NI+ RIDDLLYVDDLTK SD       P +S   I+A
Sbjct: 432 QWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVS--SIIA 491

BLAST of Lcy06g022740 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 480.7 bits (1236), Expect = 2.6e-134
Identity = 251/425 (59.06%), Postives = 312/425 (73.41%), Query Frame = 0

Query: 173 SSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPER 232
           + +SE EMMKERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LWRLEPLAP++
Sbjct: 86  NDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQK 145

Query: 233 KAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLL 292
           KAMWRRE+EWLLCVS+ IVELIP  Q FP G   EIM  RPRSDL  NLPAL+KLD ML+
Sbjct: 146 KAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLI 205

Query: 293 DLLDSFVDSEFWYVDQGILAAEADGSSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQL 352
           D+LD+F D+EFWY D+GI+  + D  S       RQEDKWWLP P+VP  GLSE +R++L
Sbjct: 206 DMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKL 265

Query: 353 QHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVC 412
           Q  RD  NQILKAA+AINS  LA+M++P  YLE LPKSG+  LGE IY+Y+++ +FSP C
Sbjct: 266 QQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPEC 325

Query: 413 LLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSW-EMLKELVIDA 472
           LL+CLD+SSEHQ +EIANR+EAA++ WR+        H        SSW   +K LV D 
Sbjct: 326 LLDCLDLSSEHQTLEIANRIEAAVHVWRQ---KNGRRHKKQAKLKLSSWGGKVKGLVNDN 385

Query: 473 DKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV 532
           ++     +RAE+LL  L+ +FP LPQT+LDM+KIQ+NKDVG++ILESYSRV+ES+AFNI 
Sbjct: 386 ERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNIT 445

Query: 533 TRIDDLLYVDDLTKHSDQIPGISQLGIVAHNSSN-RIQFSVPFCSSPYSATFMKPSFSAV 592
            RIDD+LYVDD  + S  I     L + + N  N +  FSV   SSP+ + F  P+ S V
Sbjct: 446 ARIDDVLYVDDAMRRS--ISVTESLSLFSINGLNPQKAFSVQ--SSPHGSPFATPALS-V 502

Query: 593 GTRSP 596
            +RSP
Sbjct: 506 ASRSP 502

BLAST of Lcy06g022740 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.3e-127
Identity = 274/553 (49.55%), Postives = 366/553 (66.18%), Query Frame = 0

Query: 96  EEKCEGFDNARKVFDEKS----------GEEESGSSSDLLTSEATGCEGRSQDEESSSSS 155
           +  C GF+ +++ F E S           E  + SS    +S      GRS   E S SS
Sbjct: 3   DNSCIGFEGSKR-FGETSKRIIGLIDSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSS 62

Query: 156 ----STLMGWPVHQRAAEEGN----------FDGRELERQVSSVSETEMMKERFSKLLLG 215
               S ++GWP+ Q +  + +           D    +R  +  SE E++KER +KLLLG
Sbjct: 63  PPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLG 122

Query: 216 EDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMWRREMEWLLCVSNHIV 275
           EDMSG G GVC ALAISNAITNL A + GQ WRLEP+  E+K MWRRE+E LL VS+HIV
Sbjct: 123 EDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIV 182

Query: 276 ELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLDSFVDSEFWYVDQGIL 335
           EL+P +Q FP+G+K+E+M CRPRSDL   LPALRKLD+ML+++LDSF ++EFWYVDQGI+
Sbjct: 183 ELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIV 242

Query: 336 AAEADGSSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQLQHKRDCTNQILKAAMAINS 395
           AAE+  S+SFR+      DKWWLP+PRVPS GL+E +R++L H R+ TNQILKA M+INS
Sbjct: 243 AAESARSNSFRE----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSINS 302

Query: 396 VTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLLECLDMSSEHQAIEIANR 455
           + LA+M+VP SYLEALPK+GR+ LG+ +YR I+S+ FS   LLE +D+SSE   +E+ANR
Sbjct: 303 IALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMANR 362

Query: 456 VEAAMYAWRKGTTSKPTNHNSS--RSTSKSSWEMLKELVI---DADKTQLFAERAESLLF 515
           VEA+MY WR+   S+   H  S  RSTS     ++KE+++   D DK ++FAERAESLL 
Sbjct: 363 VEASMYVWRRRAHSR---HLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLLI 422

Query: 516 CLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRIDDLLYVDDLTKH 575
            LKQ+FP L QT+LD SKIQ+NKDVGK+ILESYSRVLESLA++I  RI+++L++DD++K 
Sbjct: 423 RLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISKD 482

Query: 576 ---------SDQIPGISQLGIVAHNSSNRIQFSVPF--------CSSPY---SATFMKPS 600
                    SD++  +S+      + S R + S P          S+PY   S +   PS
Sbjct: 483 DGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFSASTPS 542

BLAST of Lcy06g022740 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 2.1e-120
Identity = 235/436 (53.90%), Postives = 315/436 (72.25%), Query Frame = 0

Query: 127 SEAT-GCEGRSQDEESSSSSSTLMGWPVHQRAAEEGNFDGRELERQ----VSSVSETEM- 186
           SE T G   +  +  S  S ++   WPV   +       G E++      V  +SE E+ 
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNWPVLTESKSSKCLSGLEMQSNECLVVQEISEPELE 106

Query: 187 -MKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMWRRE 246
            MKERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEPL  E++A+W+RE
Sbjct: 107 TMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKRE 166

Query: 247 MEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLDSFV 306
           M  LL V ++IVE IP  Q   +G+ +E+M  RPR+D+ +NLPALRKLD ML++ LDSF 
Sbjct: 167 MNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQ 226

Query: 307 DSEFWYVDQGILAAEADGSS--SFRKVL-ERQEDKWWLPIPRVPSGGLSEPSRRQLQHKR 366
           ++EFWY ++G L+ ++  SS  SFRKV+ +R+E+KWWLP+P VPS GLS+ +R+QL++KR
Sbjct: 227 NTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKR 286

Query: 367 DCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYIS-SEEFSPVCLLE 426
           + TNQI KAAMAINS  L++M++P SY+  LPK G++S+G++IYRY+S S  F P  LL+
Sbjct: 287 ESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLD 346

Query: 427 CLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVID---AD 486
           CL++SSEH+A+++A+RVEA+MY WR+          S  S SK+SW M+K+L+      D
Sbjct: 347 CLNISSEHEAVQLADRVEASMYTWRR---------KSCLSNSKNSWNMVKDLMSTTERTD 406

Query: 487 KTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVT 546
           K  + AERAE+LLFCLKQ++P L QTSLD+ KIQ+NKDVGKA+LESYSRVLE LAFNIV 
Sbjct: 407 KNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVA 466

Query: 547 RIDDLLYVDDLTKHSD 549
            IDD+LYVD   + S+
Sbjct: 467 WIDDVLYVDKTMRGSE 473

BLAST of Lcy06g022740 vs. ExPASy TrEMBL
Match: A0A6J1CWY5 (rop guanine nucleotide exchange factor 7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 9.8e-278
Identity = 521/667 (78.11%), Postives = 557/667 (83.51%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MD+ F HQ+    PIR  +HSLKLCLP +     DC NPFSIL  WV KSARK+     F
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNL--VNHDCSNPFSILRRWVWKSARKV-----F 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAME--------TEEKCEGFDNARKVFDEK 120
            SGFCC GLEYKGMVVNNSTFCSSP FLEEVAME         EEKC+ F NA KVFDEK
Sbjct: 61  PSGFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEK 120

Query: 121 --SGEEESGSSSDLLTSEATGCEGRSQDEESSSSSSTLMGWPVHQRAAEEG-----NFDG 180
              GE  S SSSDLLTSEATG    S DEE  SSSSTLMGWP++Q   EEG     +FD 
Sbjct: 121 RGDGESASSSSSDLLTSEATG----SHDEE--SSSSTLMGWPLNQTVGEEGGGDKLHFDD 180

Query: 181 RELERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRL 240
           RELER+ SSVSE E+M ERFSKLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL
Sbjct: 181 RELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240

Query: 241 EPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALR 300
           EPLA ERK MWRREMEWLLCVSNHIVELIP+WQTFPDGSKLEIMTCRPRSDL  NLPALR
Sbjct: 241 EPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALR 300

Query: 301 KLDHMLLDLLDSFVDSEFWYVDQGILAAEADGSSSFRKVLERQEDKWWLPIPRVPSGGLS 360
           KLDHMLLD+LDSFVDSEFWYVDQGILAAEADG SSFRK+LERQEDKWWLP+PRVPSGGLS
Sbjct: 301 KLDHMLLDILDSFVDSEFWYVDQGILAAEADG-SSFRKLLERQEDKWWLPVPRVPSGGLS 360

Query: 361 EPSRRQLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISS 420
           E SRRQLQHKRDCTNQILKAAMAINSVTLADMDVP SYLEALPK+GRASLGE IYR+ISS
Sbjct: 361 EASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISS 420

Query: 421 EEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLK 480
           +EFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKG  SKPTN NS RS++KSSWEMLK
Sbjct: 421 DEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTN-NSLRSSAKSSWEMLK 480

Query: 481 ELVIDADKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLES 540
           ELVIDADK++LFAERAES+L+CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLES
Sbjct: 481 ELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLES 540

Query: 541 LAFNIVTRIDDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKP 600
           LAFNIV RIDDLLYVDDL KHSD+IP ISQLGIVAHN+SNRIQFSVPF SSPY+ TF KP
Sbjct: 541 LAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKP 600

Query: 601 SFSAVGTRSPIPVGGQASQCGVVVK-----NVMNMKGCGDLIEKLDLVAEIVEE--SEKS 646
           SF      SPIP+  Q    G+ V      NV +MKGCG+ IEKLDLVAEI E+  SE+S
Sbjct: 601 SF------SPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERS 645

BLAST of Lcy06g022740 vs. ExPASy TrEMBL
Match: A0A6J1CV30 (rop guanine nucleotide exchange factor 7-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 3.2e-273
Identity = 521/695 (74.96%), Postives = 557/695 (80.14%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MD+ F HQ+    PIR  +HSLKLCLP +     DC NPFSIL  WV KSARK+     F
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNL--VNHDCSNPFSILRRWVWKSARKV-----F 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAME--------TEEKCEGFDNARKVFDEK 120
            SGFCC GLEYKGMVVNNSTFCSSP FLEEVAME         EEKC+ F NA KVFDEK
Sbjct: 61  PSGFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEK 120

Query: 121 --SGEEESGSSSDLLTSEATGCEGRSQDEESSSSSSTLMGWPVHQRAAEEG-----NFDG 180
              GE  S SSSDLLTSEATG    S DEE  SSSSTLMGWP++Q   EEG     +FD 
Sbjct: 121 RGDGESASSSSSDLLTSEATG----SHDEE--SSSSTLMGWPLNQTVGEEGGGDKLHFDD 180

Query: 181 RELERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRL 240
           RELER+ SSVSE E+M ERFSKLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL
Sbjct: 181 RELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240

Query: 241 EPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLE------------------ 300
           EPLA ERK MWRREMEWLLCVSNHIVELIP+WQTFPDGSKLE                  
Sbjct: 241 EPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEVQFKISKYLFTKFLSLQL 300

Query: 301 ----------IMTCRPRSDLCVNLPALRKLDHMLLDLLDSFVDSEFWYVDQGILAAEADG 360
                     IMTCRPRSDL  NLPALRKLDHMLLD+LDSFVDSEFWYVDQGILAAEADG
Sbjct: 301 FFIFFLLADQIMTCRPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADG 360

Query: 361 SSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQLQHKRDCTNQILKAAMAINSVTLADM 420
            SSFRK+LERQEDKWWLP+PRVPSGGLSE SRRQLQHKRDCTNQILKAAMAINSVTLADM
Sbjct: 361 -SSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADM 420

Query: 421 DVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLLECLDMSSEHQAIEIANRVEAAMY 480
           DVP SYLEALPK+GRASLGE IYR+ISS+EFSPVCLLECLDMSSEHQAIEIANRVEAAMY
Sbjct: 421 DVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMY 480

Query: 481 AWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADKTQLFAERAESLLFCLKQQFPNLPQ 540
           AWRKG  SKPTN NS RS++KSSWEMLKELVIDADK++LFAERAES+L+CLKQQFPNLPQ
Sbjct: 481 AWRKGMGSKPTN-NSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQ 540

Query: 541 TSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRIDDLLYVDDLTKHSDQIPGISQLG 600
           T+LDMSKIQ NKDVGKAILESYSRVLESLAFNIV RIDDLLYVDDL KHSD+IP ISQLG
Sbjct: 541 TNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLG 600

Query: 601 IVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAVGTRSPIPVGGQASQCGVVVK-----NV 646
           IVAHN+SNRIQFSVPF SSPY+ TF KPSF      SPIP+  Q    G+ V      NV
Sbjct: 601 IVAHNTSNRIQFSVPFSSSPYTTTFTKPSF------SPIPIISQTPHRGIGVNNESIDNV 660

BLAST of Lcy06g022740 vs. ExPASy TrEMBL
Match: A0A6J1FND9 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111447031 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.5e-251
Identity = 475/610 (77.87%), Postives = 517/610 (84.75%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MDLIFTHQ++ + P+ +T+HSL         SGSD FNPF  L  WVSKS++KL  RARF
Sbjct: 1   MDLIFTHQEERQ-PVPITNHSL-------NRSGSDRFNPFLTLSCWVSKSSQKLRHRARF 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAMETEEKCEGFDNARKVFDEKSGEEESG- 120
           S+GFCCGG+EYKGMVVNNSTFCSSPVFLEEVA+ET EKC+GF +ARKVFDEK+G  ES  
Sbjct: 61  STGFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETGEKCDGFGDARKVFDEKTGVGESSL 120

Query: 121 --SSSDLLTSEATGCEGRSQDEE-SSSSSSTLMGWPVHQRAAEEGNFDGRELERQVSSVS 180
             SSSDLL+SEATG EG+S +EE SSSSSSTLMGWP+H+ AAEE NFDGR+LE QVS VS
Sbjct: 121 SDSSSDLLSSEATGHEGQSHEEESSSSSSSTLMGWPLHRTAAEERNFDGRQLETQVSPVS 180

Query: 181 ETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMW 240
           ETEMMKERF+KLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL+PLA   KAMW
Sbjct: 181 ETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLQPLASHIKAMW 240

Query: 241 RREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLD 300
           +REME LL V+NHIV+LIP+ QTFPDG+KLEIMT RPRSDL V+LPALRKLDHMLLD+LD
Sbjct: 241 QREMELLLSVTNHIVQLIPVSQTFPDGTKLEIMTSRPRSDLYVSLPALRKLDHMLLDILD 300

Query: 301 SFVDSEFWYVDQGIL-AAEADGSSSFRKVLERQEDKWWLPIPRVPSG-GLSEPSRRQLQH 360
           SFVDS FWYVDQGIL AA+AD SSS      RQEDKWWLPIPRVPSG GLSE +RRQLQH
Sbjct: 301 SFVDSAFWYVDQGILAAADADRSSS------RQEDKWWLPIPRVPSGSGLSEAARRQLQH 360

Query: 361 KRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLL 420
           KRD TNQILKA MAINS+TLADMDVP SYLEALPK+GR SLGE IYRYISS+EFSPV +L
Sbjct: 361 KRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYISSDEFSPVYVL 420

Query: 421 ECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADKT 480
           ECLD+SSEHQA+EIANRVEAAMYAWR          NSSRSTSKSSWEMLKELV+DA+K+
Sbjct: 421 ECLDISSEHQAVEIANRVEAAMYAWR----------NSSRSTSKSSWEMLKELVVDAEKS 480

Query: 481 QLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRI 540
           Q+FAERAESLL CLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV RI
Sbjct: 481 QVFAERAESLLLCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVARI 540

Query: 541 DDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAV---- 597
           DDL+YVDDLTKHSDQIPGISQLGI     S RIQF VPF SSP    F KPSFSAV    
Sbjct: 541 DDLVYVDDLTKHSDQIPGISQLGI-----SGRIQFPVPFSSSP----FTKPSFSAVDLVT 577

BLAST of Lcy06g022740 vs. ExPASy TrEMBL
Match: A0A6J1I2R8 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111470019 PE=4 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 3.3e-249
Identity = 470/611 (76.92%), Postives = 513/611 (83.96%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MDLIFTHQ++ E P+ +T+HSL           SD FNPF  L  WVSKSA+KL  RARF
Sbjct: 1   MDLIFTHQEERE-PVPITNHSLNRI-------SSDRFNPFLTLSYWVSKSAQKLRHRARF 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAMETEEKCEGFDNARKVFDEKSGEEESG- 120
           S+GFCCGG+EYKGMVVNNSTFCSSPVFLEEVA+ET EKC+GF +A KVFDEKSG  ES  
Sbjct: 61  STGFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETGEKCDGFGDALKVFDEKSGVGESSL 120

Query: 121 --SSSDLLTSEATGCEGRSQDEE---SSSSSSTLMGWPVHQRAAEEGNFDGRELERQVSS 180
             SS DLL+SEATG EG+S +EE   SSSSSSTLMGWP+H+ AAEE NFD R+LE QVS 
Sbjct: 121 SDSSPDLLSSEATGHEGQSHEEEPSSSSSSSSTLMGWPLHRTAAEERNFDDRQLETQVSP 180

Query: 181 VSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKA 240
           VSETEMMKERF+KLLLGEDMSGCGNGV TALAISNAITNLCA+LFGQLWRL+PLAP +KA
Sbjct: 181 VSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCASLFGQLWRLQPLAPHKKA 240

Query: 241 MWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDL 300
           MW+REME LL V+NHIV+LIPI QTFPDG+KLEIMT RPRSDL V+LPALRKLD MLLD+
Sbjct: 241 MWQREMELLLSVTNHIVQLIPISQTFPDGTKLEIMTSRPRSDLYVSLPALRKLDRMLLDI 300

Query: 301 LDSFVDSEFWYVDQGILAAEADGSSSFRKVLERQEDKWWLPIPRVPSG-GLSEPSRRQLQ 360
           LDSFVDSEFWYVDQGILAA+ D SSS      RQEDKWWLPIPRVPSG GLSE +RRQLQ
Sbjct: 301 LDSFVDSEFWYVDQGILAADTDRSSS------RQEDKWWLPIPRVPSGSGLSEAARRQLQ 360

Query: 361 HKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCL 420
           HKRD TNQILKA MAINS+TLADMDVP SYLEALPK+GR SLGE IYRYISS+EFSPV +
Sbjct: 361 HKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYISSDEFSPVYV 420

Query: 421 LECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADK 480
           LEC+D+SSEHQA+EIANRVEAAMYAWR          NSSRSTSKSSWEMLKELV+DA+K
Sbjct: 421 LECVDISSEHQAVEIANRVEAAMYAWR----------NSSRSTSKSSWEMLKELVVDAEK 480

Query: 481 TQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTR 540
           +Q+FAERAES+LFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV R
Sbjct: 481 SQVFAERAESVLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVAR 540

Query: 541 IDDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAV--- 597
           IDDL+YVDDLTKHSDQIPGISQLG+     S RIQF VPF SSP    F K SFSAV   
Sbjct: 541 IDDLVYVDDLTKHSDQIPGISQLGV-----SGRIQFPVPFSSSP----FTKLSFSAVDLV 578

BLAST of Lcy06g022740 vs. ExPASy TrEMBL
Match: A0A5A7TMM7 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004810 PE=4 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 5.2e-247
Identity = 479/669 (71.60%), Postives = 528/669 (78.92%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MDL FTHQQ  E PI +T+HS         P+ SDCFNPFS LG WVSKSARKLC RAR 
Sbjct: 1   MDLTFTHQQHIE-PIPLTNHS---------PTPSDCFNPFSNLGNWVSKSARKLCHRARS 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAME---TEEKCEGFDNARKVFDEKSGEEE 120
           S+GFCC GLEY+GMVVNNS       FLE VAME    EEKC+ F+NARKV DEK+    
Sbjct: 61  STGFCCRGLEYRGMVVNNS------AFLEVVAMEIEAKEEKCDVFENARKVSDEKTA--- 120

Query: 121 SGSSSDLLTSEATG--CEGRSQDEESSSSSST-----------LMGWPVHQRAAEEGNFD 180
           S SSSDLL S  T    E  S+DEESSSSSS+            MGWP+H+R   EG  D
Sbjct: 121 SNSSSDLLKSVETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVG-EGEED 180

Query: 181 GRELERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWR 240
           G  +E++VS+ SE EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC ++FGQLWR
Sbjct: 181 G--VEKEVSTDSEMEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWR 240

Query: 241 LEPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPAL 300
           LEPL  ERKAMW REME+LLCVSNHIVELIPIWQTFPDG+KLEIMTCRPRSDL VNLPAL
Sbjct: 241 LEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPAL 300

Query: 301 RKLDHMLLDLLDSFVDSEFWYVDQGILAA-EADGSSSFRKVLERQEDKWWLPIPRVPSGG 360
           RKLDHMLLD+LDSFVDSEFWY+DQGILA  +AD SSSFRK+LERQ+DKWWLPIPRVP+GG
Sbjct: 301 RKLDHMLLDMLDSFVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGG 360

Query: 361 LSEPSRRQLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYI 420
           LSE S R L HKRDCTNQILKAAMAINSVTLADMDVPISYLEALPK+GRASLGEAIY+YI
Sbjct: 361 LSEASTRHLHHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYI 420

Query: 421 SSEEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWR-KGTTSKPTNHNSSRSTSKSSWE 480
           SS+EFSP  LLECLD+SSEHQAIEIANRVE+AMYAWR KG  S         + SKSSWE
Sbjct: 421 SSDEFSPAWLLECLDISSEHQAIEIANRVESAMYAWRSKGIAS---------NNSKSSWE 480

Query: 481 MLKELVIDADKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRV 540
           MLKEL+IDADK+++ AERAE +L CLKQQFPNLPQTSLDMSKIQ+NKDVGKAILESYSRV
Sbjct: 481 MLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRV 540

Query: 541 LESLAFNIVTRIDDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATF 600
           LESLA+NIV RI+DLLYV++LTKHSDQIPGISQLGIVAHNSSNRIQ SVPF +SPY+  F
Sbjct: 541 LESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKF 600

Query: 601 MKPSFSA---VGTRSPIPVGGQASQCGVVVKNVMNMKGCGDLIEKLDLVAEIVEESEKSE 649
           +KPSFSA   VGTRSP P   +  QCG                         +EESEKSE
Sbjct: 601 IKPSFSAVDLVGTRSPTP---KPPQCG-------------------------LEESEKSE 610

BLAST of Lcy06g022740 vs. NCBI nr
Match: XP_022145651.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Momordica charantia])

HSP 1 Score: 965.7 bits (2495), Expect = 2.0e-277
Identity = 521/667 (78.11%), Postives = 557/667 (83.51%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MD+ F HQ+    PIR  +HSLKLCLP +     DC NPFSIL  WV KSARK+     F
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNL--VNHDCSNPFSILRRWVWKSARKV-----F 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAME--------TEEKCEGFDNARKVFDEK 120
            SGFCC GLEYKGMVVNNSTFCSSP FLEEVAME         EEKC+ F NA KVFDEK
Sbjct: 61  PSGFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEK 120

Query: 121 --SGEEESGSSSDLLTSEATGCEGRSQDEESSSSSSTLMGWPVHQRAAEEG-----NFDG 180
              GE  S SSSDLLTSEATG    S DEE  SSSSTLMGWP++Q   EEG     +FD 
Sbjct: 121 RGDGESASSSSSDLLTSEATG----SHDEE--SSSSTLMGWPLNQTVGEEGGGDKLHFDD 180

Query: 181 RELERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRL 240
           RELER+ SSVSE E+M ERFSKLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL
Sbjct: 181 RELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240

Query: 241 EPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALR 300
           EPLA ERK MWRREMEWLLCVSNHIVELIP+WQTFPDGSKLEIMTCRPRSDL  NLPALR
Sbjct: 241 EPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALR 300

Query: 301 KLDHMLLDLLDSFVDSEFWYVDQGILAAEADGSSSFRKVLERQEDKWWLPIPRVPSGGLS 360
           KLDHMLLD+LDSFVDSEFWYVDQGILAAEADG SSFRK+LERQEDKWWLP+PRVPSGGLS
Sbjct: 301 KLDHMLLDILDSFVDSEFWYVDQGILAAEADG-SSFRKLLERQEDKWWLPVPRVPSGGLS 360

Query: 361 EPSRRQLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISS 420
           E SRRQLQHKRDCTNQILKAAMAINSVTLADMDVP SYLEALPK+GRASLGE IYR+ISS
Sbjct: 361 EASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISS 420

Query: 421 EEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLK 480
           +EFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKG  SKPTN NS RS++KSSWEMLK
Sbjct: 421 DEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTN-NSLRSSAKSSWEMLK 480

Query: 481 ELVIDADKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLES 540
           ELVIDADK++LFAERAES+L+CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLES
Sbjct: 481 ELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLES 540

Query: 541 LAFNIVTRIDDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKP 600
           LAFNIV RIDDLLYVDDL KHSD+IP ISQLGIVAHN+SNRIQFSVPF SSPY+ TF KP
Sbjct: 541 LAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKP 600

Query: 601 SFSAVGTRSPIPVGGQASQCGVVVK-----NVMNMKGCGDLIEKLDLVAEIVEE--SEKS 646
           SF      SPIP+  Q    G+ V      NV +MKGCG+ IEKLDLVAEI E+  SE+S
Sbjct: 601 SF------SPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERS 645

BLAST of Lcy06g022740 vs. NCBI nr
Match: XP_022145650.1 (rop guanine nucleotide exchange factor 7-like isoform X1 [Momordica charantia])

HSP 1 Score: 950.7 bits (2456), Expect = 6.7e-273
Identity = 521/695 (74.96%), Postives = 557/695 (80.14%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MD+ F HQ+    PIR  +HSLKLCLP +     DC NPFSIL  WV KSARK+     F
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNL--VNHDCSNPFSILRRWVWKSARKV-----F 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAME--------TEEKCEGFDNARKVFDEK 120
            SGFCC GLEYKGMVVNNSTFCSSP FLEEVAME         EEKC+ F NA KVFDEK
Sbjct: 61  PSGFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEK 120

Query: 121 --SGEEESGSSSDLLTSEATGCEGRSQDEESSSSSSTLMGWPVHQRAAEEG-----NFDG 180
              GE  S SSSDLLTSEATG    S DEE  SSSSTLMGWP++Q   EEG     +FD 
Sbjct: 121 RGDGESASSSSSDLLTSEATG----SHDEE--SSSSTLMGWPLNQTVGEEGGGDKLHFDD 180

Query: 181 RELERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRL 240
           RELER+ SSVSE E+M ERFSKLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL
Sbjct: 181 RELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240

Query: 241 EPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLE------------------ 300
           EPLA ERK MWRREMEWLLCVSNHIVELIP+WQTFPDGSKLE                  
Sbjct: 241 EPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEVQFKISKYLFTKFLSLQL 300

Query: 301 ----------IMTCRPRSDLCVNLPALRKLDHMLLDLLDSFVDSEFWYVDQGILAAEADG 360
                     IMTCRPRSDL  NLPALRKLDHMLLD+LDSFVDSEFWYVDQGILAAEADG
Sbjct: 301 FFIFFLLADQIMTCRPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADG 360

Query: 361 SSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQLQHKRDCTNQILKAAMAINSVTLADM 420
            SSFRK+LERQEDKWWLP+PRVPSGGLSE SRRQLQHKRDCTNQILKAAMAINSVTLADM
Sbjct: 361 -SSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADM 420

Query: 421 DVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLLECLDMSSEHQAIEIANRVEAAMY 480
           DVP SYLEALPK+GRASLGE IYR+ISS+EFSPVCLLECLDMSSEHQAIEIANRVEAAMY
Sbjct: 421 DVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMY 480

Query: 481 AWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADKTQLFAERAESLLFCLKQQFPNLPQ 540
           AWRKG  SKPTN NS RS++KSSWEMLKELVIDADK++LFAERAES+L+CLKQQFPNLPQ
Sbjct: 481 AWRKGMGSKPTN-NSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQ 540

Query: 541 TSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRIDDLLYVDDLTKHSDQIPGISQLG 600
           T+LDMSKIQ NKDVGKAILESYSRVLESLAFNIV RIDDLLYVDDL KHSD+IP ISQLG
Sbjct: 541 TNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLG 600

Query: 601 IVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAVGTRSPIPVGGQASQCGVVVK-----NV 646
           IVAHN+SNRIQFSVPF SSPY+ TF KPSF      SPIP+  Q    G+ V      NV
Sbjct: 601 IVAHNTSNRIQFSVPFSSSPYTTTFTKPSF------SPIPIISQTPHRGIGVNNESIDNV 660

BLAST of Lcy06g022740 vs. NCBI nr
Match: KAG6596381.1 (Rop guanine nucleotide exchange factor 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027930.1 Rop guanine nucleotide exchange factor 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 882.9 bits (2280), Expect = 1.7e-252
Identity = 477/610 (78.20%), Postives = 519/610 (85.08%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MDLIFTHQ++ + P+ +T+HSL         SGSD FNPF  L  WVSKS++KL  RARF
Sbjct: 1   MDLIFTHQEERQ-PVPITNHSL-------NRSGSDRFNPFLTLSCWVSKSSQKLRHRARF 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAMETEEKCEGFDNARKVFDEKSGEEESG- 120
           S+GFCCGG+EYKGMVVNNSTFCSSPVFLEEVA+ET EKC+GF +ARKVFDEK+G  ES  
Sbjct: 61  STGFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETGEKCDGFGDARKVFDEKTGVGESSL 120

Query: 121 --SSSDLLTSEATGCEGRSQDEE-SSSSSSTLMGWPVHQRAAEEGNFDGRELERQVSSVS 180
             SSSDLL+SEATG EG+S +EE SSSSSSTLMGWP+H+ AAEE NFDGR+LE QVS VS
Sbjct: 121 SDSSSDLLSSEATGHEGQSHEEESSSSSSSTLMGWPLHRTAAEERNFDGRQLETQVSPVS 180

Query: 181 ETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMW 240
           ETEMMKERF+KLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL+PLAP  KAMW
Sbjct: 181 ETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLQPLAPHIKAMW 240

Query: 241 RREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLD 300
           +REME LL V+NHIV+LIP+ QTFPDG+KLEIMT RPRSDL V+LPALRKLDHMLLD+LD
Sbjct: 241 QREMELLLSVTNHIVQLIPVSQTFPDGTKLEIMTSRPRSDLYVSLPALRKLDHMLLDILD 300

Query: 301 SFVDSEFWYVDQGIL-AAEADGSSSFRKVLERQEDKWWLPIPRVPSG-GLSEPSRRQLQH 360
           SFVDSEFWYVDQGIL AA+AD SSS      RQEDKWWLPIPRVPSG GLSE +RRQLQH
Sbjct: 301 SFVDSEFWYVDQGILAAADADRSSS------RQEDKWWLPIPRVPSGSGLSEAARRQLQH 360

Query: 361 KRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLL 420
           KRD TNQILKA MAINS+TLADMDVP SYLEALPK+GR SLGE IYRYISS+EFSPV +L
Sbjct: 361 KRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYISSDEFSPVYVL 420

Query: 421 ECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADKT 480
           ECLD+SSEHQA+EIANRVEAAMYAWR          NSSRSTSKSSWEMLKELV+DA+K+
Sbjct: 421 ECLDISSEHQAVEIANRVEAAMYAWR----------NSSRSTSKSSWEMLKELVVDAEKS 480

Query: 481 QLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRI 540
           Q+FAERAESLL CLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV RI
Sbjct: 481 QVFAERAESLLLCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVARI 540

Query: 541 DDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAV---- 597
           DDL+YVDDLTKHSDQIPGISQLGI     S RIQF VPF SSP    F KPSFSAV    
Sbjct: 541 DDLVYVDDLTKHSDQIPGISQLGI-----SGRIQFPVPFSSSP----FTKPSFSAVDLVT 577

BLAST of Lcy06g022740 vs. NCBI nr
Match: XP_022941762.1 (rop guanine nucleotide exchange factor 7-like [Cucurbita moschata])

HSP 1 Score: 877.5 bits (2266), Expect = 7.2e-251
Identity = 475/610 (77.87%), Postives = 517/610 (84.75%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MDLIFTHQ++ + P+ +T+HSL         SGSD FNPF  L  WVSKS++KL  RARF
Sbjct: 1   MDLIFTHQEERQ-PVPITNHSL-------NRSGSDRFNPFLTLSCWVSKSSQKLRHRARF 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAMETEEKCEGFDNARKVFDEKSGEEESG- 120
           S+GFCCGG+EYKGMVVNNSTFCSSPVFLEEVA+ET EKC+GF +ARKVFDEK+G  ES  
Sbjct: 61  STGFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETGEKCDGFGDARKVFDEKTGVGESSL 120

Query: 121 --SSSDLLTSEATGCEGRSQDEE-SSSSSSTLMGWPVHQRAAEEGNFDGRELERQVSSVS 180
             SSSDLL+SEATG EG+S +EE SSSSSSTLMGWP+H+ AAEE NFDGR+LE QVS VS
Sbjct: 121 SDSSSDLLSSEATGHEGQSHEEESSSSSSSTLMGWPLHRTAAEERNFDGRQLETQVSPVS 180

Query: 181 ETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMW 240
           ETEMMKERF+KLLLGEDMSGCGNGV TALAISNAITNLCATLFGQLWRL+PLA   KAMW
Sbjct: 181 ETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLQPLASHIKAMW 240

Query: 241 RREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLD 300
           +REME LL V+NHIV+LIP+ QTFPDG+KLEIMT RPRSDL V+LPALRKLDHMLLD+LD
Sbjct: 241 QREMELLLSVTNHIVQLIPVSQTFPDGTKLEIMTSRPRSDLYVSLPALRKLDHMLLDILD 300

Query: 301 SFVDSEFWYVDQGIL-AAEADGSSSFRKVLERQEDKWWLPIPRVPSG-GLSEPSRRQLQH 360
           SFVDS FWYVDQGIL AA+AD SSS      RQEDKWWLPIPRVPSG GLSE +RRQLQH
Sbjct: 301 SFVDSAFWYVDQGILAAADADRSSS------RQEDKWWLPIPRVPSGSGLSEAARRQLQH 360

Query: 361 KRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLL 420
           KRD TNQILKA MAINS+TLADMDVP SYLEALPK+GR SLGE IYRYISS+EFSPV +L
Sbjct: 361 KRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYISSDEFSPVYVL 420

Query: 421 ECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADKT 480
           ECLD+SSEHQA+EIANRVEAAMYAWR          NSSRSTSKSSWEMLKELV+DA+K+
Sbjct: 421 ECLDISSEHQAVEIANRVEAAMYAWR----------NSSRSTSKSSWEMLKELVVDAEKS 480

Query: 481 QLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRI 540
           Q+FAERAESLL CLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV RI
Sbjct: 481 QVFAERAESLLLCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVARI 540

Query: 541 DDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAV---- 597
           DDL+YVDDLTKHSDQIPGISQLGI     S RIQF VPF SSP    F KPSFSAV    
Sbjct: 541 DDLVYVDDLTKHSDQIPGISQLGI-----SGRIQFPVPFSSSP----FTKPSFSAVDLVT 577

BLAST of Lcy06g022740 vs. NCBI nr
Match: XP_022971231.1 (rop guanine nucleotide exchange factor 7-like [Cucurbita maxima])

HSP 1 Score: 870.9 bits (2249), Expect = 6.8e-249
Identity = 470/611 (76.92%), Postives = 513/611 (83.96%), Query Frame = 0

Query: 1   MDLIFTHQQDTEPPIRVTSHSLKLCLPLVRPSGSDCFNPFSILGIWVSKSARKLCSRARF 60
           MDLIFTHQ++ E P+ +T+HSL           SD FNPF  L  WVSKSA+KL  RARF
Sbjct: 1   MDLIFTHQEERE-PVPITNHSLNRI-------SSDRFNPFLTLSYWVSKSAQKLRHRARF 60

Query: 61  SSGFCCGGLEYKGMVVNNSTFCSSPVFLEEVAMETEEKCEGFDNARKVFDEKSGEEESG- 120
           S+GFCCGG+EYKGMVVNNSTFCSSPVFLEEVA+ET EKC+GF +A KVFDEKSG  ES  
Sbjct: 61  STGFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETGEKCDGFGDALKVFDEKSGVGESSL 120

Query: 121 --SSSDLLTSEATGCEGRSQDEE---SSSSSSTLMGWPVHQRAAEEGNFDGRELERQVSS 180
             SS DLL+SEATG EG+S +EE   SSSSSSTLMGWP+H+ AAEE NFD R+LE QVS 
Sbjct: 121 SDSSPDLLSSEATGHEGQSHEEEPSSSSSSSSTLMGWPLHRTAAEERNFDDRQLETQVSP 180

Query: 181 VSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKA 240
           VSETEMMKERF+KLLLGEDMSGCGNGV TALAISNAITNLCA+LFGQLWRL+PLAP +KA
Sbjct: 181 VSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCASLFGQLWRLQPLAPHKKA 240

Query: 241 MWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDL 300
           MW+REME LL V+NHIV+LIPI QTFPDG+KLEIMT RPRSDL V+LPALRKLD MLLD+
Sbjct: 241 MWQREMELLLSVTNHIVQLIPISQTFPDGTKLEIMTSRPRSDLYVSLPALRKLDRMLLDI 300

Query: 301 LDSFVDSEFWYVDQGILAAEADGSSSFRKVLERQEDKWWLPIPRVPSG-GLSEPSRRQLQ 360
           LDSFVDSEFWYVDQGILAA+ D SSS      RQEDKWWLPIPRVPSG GLSE +RRQLQ
Sbjct: 301 LDSFVDSEFWYVDQGILAADTDRSSS------RQEDKWWLPIPRVPSGSGLSEAARRQLQ 360

Query: 361 HKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCL 420
           HKRD TNQILKA MAINS+TLADMDVP SYLEALPK+GR SLGE IYRYISS+EFSPV +
Sbjct: 361 HKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYISSDEFSPVYV 420

Query: 421 LECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDADK 480
           LEC+D+SSEHQA+EIANRVEAAMYAWR          NSSRSTSKSSWEMLKELV+DA+K
Sbjct: 421 LECVDISSEHQAVEIANRVEAAMYAWR----------NSSRSTSKSSWEMLKELVVDAEK 480

Query: 481 TQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTR 540
           +Q+FAERAES+LFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV R
Sbjct: 481 SQVFAERAESVLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVAR 540

Query: 541 IDDLLYVDDLTKHSDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAV--- 597
           IDDL+YVDDLTKHSDQIPGISQLG+     S RIQF VPF SSP    F K SFSAV   
Sbjct: 541 IDDLVYVDDLTKHSDQIPGISQLGV-----SGRIQFPVPFSSSP----FTKLSFSAVDLV 578

BLAST of Lcy06g022740 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 615.5 bits (1586), Expect = 4.8e-176
Identity = 325/486 (66.87%), Postives = 383/486 (78.81%), Query Frame = 0

Query: 116 EESGSSSDLLTSEATGCEGRSQDEESSSSSSTLMGWPVHQRAAEEGNFDGRELE-RQVSS 175
           EE G  S   +SE T  E   ++E+S S +      PV  R + + N DG + + R  S 
Sbjct: 12  EEKGRESSCCSSETTRQE---EEEQSPSCTEDFTASPVSSRWSVK-NIDGEKKKIRSDSR 71

Query: 176 VSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKA 235
           VSE EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLCATLFGQLWRLEPL  E+K 
Sbjct: 72  VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEKKE 131

Query: 236 MWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDL 295
           MWRREMEWLLCVS+HIVE+ P WQTFPDG+KLEIMTCRPRSDL VNLPALRKLD+MLL++
Sbjct: 132 MWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLLEI 191

Query: 296 LDSFVDSEFWYVDQGILAAE--ADGSSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQL 355
           LDSF ++EFWYVDQGI+A E  ADGSSSFRK  +RQEDKWWLP+PRV  GGL E SR+QL
Sbjct: 192 LDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRKQL 251

Query: 356 QHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVC 415
           QHKRDCTNQILKAAMAINS+TLADM++P SYLE+LP+ GR+ LG+ IYRYISS++FSP C
Sbjct: 252 QHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQFSPEC 311

Query: 416 LLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVIDAD 475
           LL+CLD+SSEHQAIEIANRVE+++Y W K T SKP       + +K+SWEM+KEL++DAD
Sbjct: 312 LLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPA------TNTKTSWEMVKELMVDAD 371

Query: 476 KTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVT 535
           K +L A+RAESLL  LKQ+FP LPQT+LDMSKIQ+NKD+GK+ILESYSRVLESLAFNIV 
Sbjct: 372 KLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVA 431

Query: 536 RIDDLLYVDDLTKH-SDQIPGISQLGIVAHNSSNRIQFSVPFCSSPYSATFMKPSFSAVG 595
           RIDDLL+VDDLT+H SDQIP  + LG    N+ N    S+    S Y+     PS   + 
Sbjct: 432 RIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTTPSYSPSKQELR 481

Query: 596 TRSPIP 598
           +   +P
Sbjct: 492 SSITVP 481

BLAST of Lcy06g022740 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 544.3 bits (1401), Expect = 1.3e-154
Identity = 286/509 (56.19%), Postives = 370/509 (72.69%), Query Frame = 0

Query: 111 EKSGEEESGSSSDLLT--SEATGCEGRSQDEESSSS---------SSTLMGWPV-----H 170
           EK     + S  D+LT   E +   G S +  S+++          S ++GWP+      
Sbjct: 12  EKKRSNLTPSMEDILTESKERSSTSGASYESSSTTTVASSSPPPPPSQILGWPIRKASFR 71

Query: 171 QRAAEEGNFDGREL--------ERQVSSVSETEMMKERFSKLLLGEDMSGCGNGVCTALA 230
           + + E  NFD ++L        + +  + ++ EMMKERF+KLLLGEDMSG G GVCTALA
Sbjct: 72  KNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALA 131

Query: 231 ISNAITNLCATLFGQLWRLEPLAPERKAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKL 290
           ISNAITNLCAT+FGQLWRLEPL+ E+K MWRREMEW+L VS+HIVEL P  QT+PDG+K 
Sbjct: 132 ISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKF 191

Query: 291 EIMTCRPRSDLCVNLPALRKLDHMLLDLLDSFVDSEFWYVDQGILAAEADGSSSFRKVLE 350
           E+MTCRPR DL +NLPALRKLD+MLLD+L SF  +EFWYVDQGI+A+E DGS+SFR+ ++
Sbjct: 192 EVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQ 251

Query: 351 RQEDKWWLPIPRVPSGGLSEPSRRQLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEA 410
           RQE+KWWLP+PR+   GL+E +R +L HKR+C  QILKAAMAINS+ L +MDVP SYLE 
Sbjct: 252 RQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLET 311

Query: 411 LPKSGRASLGEAIYRYISSEEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSK 470
           LPK+GR+ LG+ IYRY++S++FS   LL+CLD+SSEH A++IANRVEA++Y WR+   +K
Sbjct: 312 LPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTK 371

Query: 471 -PTNHNSSRSTSKSSWEMLKELVIDADKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKI 530
              N+N+S +T K +WEM+KEL+   DK  L  ER+E+LL CLKQ+FP+L QTSLD+SKI
Sbjct: 372 LGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKI 431

Query: 531 QFNKDVGKAILESYSRVLESLAFNIVTRIDDLLYVDDLTKHSD-----QIPGISQLGIVA 589
           Q+NKD+GK+ILESYSR LESLA NI+ RIDDLLYVDDLTK SD       P +S   I+A
Sbjct: 432 QWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVS--SIIA 491

BLAST of Lcy06g022740 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 480.7 bits (1236), Expect = 1.8e-135
Identity = 251/425 (59.06%), Postives = 312/425 (73.41%), Query Frame = 0

Query: 173 SSVSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPER 232
           + +SE EMMKERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LWRLEPLAP++
Sbjct: 86  NDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQK 145

Query: 233 KAMWRREMEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLL 292
           KAMWRRE+EWLLCVS+ IVELIP  Q FP G   EIM  RPRSDL  NLPAL+KLD ML+
Sbjct: 146 KAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLI 205

Query: 293 DLLDSFVDSEFWYVDQGILAAEADGSSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQL 352
           D+LD+F D+EFWY D+GI+  + D  S       RQEDKWWLP P+VP  GLSE +R++L
Sbjct: 206 DMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKL 265

Query: 353 QHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVC 412
           Q  RD  NQILKAA+AINS  LA+M++P  YLE LPKSG+  LGE IY+Y+++ +FSP C
Sbjct: 266 QQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPEC 325

Query: 413 LLECLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSW-EMLKELVIDA 472
           LL+CLD+SSEHQ +EIANR+EAA++ WR+        H        SSW   +K LV D 
Sbjct: 326 LLDCLDLSSEHQTLEIANRIEAAVHVWRQ---KNGRRHKKQAKLKLSSWGGKVKGLVNDN 385

Query: 473 DKTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIV 532
           ++     +RAE+LL  L+ +FP LPQT+LDM+KIQ+NKDVG++ILESYSRV+ES+AFNI 
Sbjct: 386 ERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNIT 445

Query: 533 TRIDDLLYVDDLTKHSDQIPGISQLGIVAHNSSN-RIQFSVPFCSSPYSATFMKPSFSAV 592
            RIDD+LYVDD  + S  I     L + + N  N +  FSV   SSP+ + F  P+ S V
Sbjct: 446 ARIDDVLYVDDAMRRS--ISVTESLSLFSINGLNPQKAFSVQ--SSPHGSPFATPALS-V 502

Query: 593 GTRSP 596
            +RSP
Sbjct: 506 ASRSP 502

BLAST of Lcy06g022740 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.7e-128
Identity = 274/553 (49.55%), Postives = 366/553 (66.18%), Query Frame = 0

Query: 96  EEKCEGFDNARKVFDEKS----------GEEESGSSSDLLTSEATGCEGRSQDEESSSSS 155
           +  C GF+ +++ F E S           E  + SS    +S      GRS   E S SS
Sbjct: 3   DNSCIGFEGSKR-FGETSKRIIGLIDSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSS 62

Query: 156 ----STLMGWPVHQRAAEEGN----------FDGRELERQVSSVSETEMMKERFSKLLLG 215
               S ++GWP+ Q +  + +           D    +R  +  SE E++KER +KLLLG
Sbjct: 63  PPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLG 122

Query: 216 EDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMWRREMEWLLCVSNHIV 275
           EDMSG G GVC ALAISNAITNL A + GQ WRLEP+  E+K MWRRE+E LL VS+HIV
Sbjct: 123 EDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIV 182

Query: 276 ELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLDSFVDSEFWYVDQGIL 335
           EL+P +Q FP+G+K+E+M CRPRSDL   LPALRKLD+ML+++LDSF ++EFWYVDQGI+
Sbjct: 183 ELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIV 242

Query: 336 AAEADGSSSFRKVLERQEDKWWLPIPRVPSGGLSEPSRRQLQHKRDCTNQILKAAMAINS 395
           AAE+  S+SFR+      DKWWLP+PRVPS GL+E +R++L H R+ TNQILKA M+INS
Sbjct: 243 AAESARSNSFRE----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSINS 302

Query: 396 VTLADMDVPISYLEALPKSGRASLGEAIYRYISSEEFSPVCLLECLDMSSEHQAIEIANR 455
           + LA+M+VP SYLEALPK+GR+ LG+ +YR I+S+ FS   LLE +D+SSE   +E+ANR
Sbjct: 303 IALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMANR 362

Query: 456 VEAAMYAWRKGTTSKPTNHNSS--RSTSKSSWEMLKELVI---DADKTQLFAERAESLLF 515
           VEA+MY WR+   S+   H  S  RSTS     ++KE+++   D DK ++FAERAESLL 
Sbjct: 363 VEASMYVWRRRAHSR---HLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLLI 422

Query: 516 CLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVTRIDDLLYVDDLTKH 575
            LKQ+FP L QT+LD SKIQ+NKDVGK+ILESYSRVLESLA++I  RI+++L++DD++K 
Sbjct: 423 RLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISKD 482

Query: 576 ---------SDQIPGISQLGIVAHNSSNRIQFSVPF--------CSSPY---SATFMKPS 600
                    SD++  +S+      + S R + S P          S+PY   S +   PS
Sbjct: 483 DGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFSASTPS 542

BLAST of Lcy06g022740 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 434.5 bits (1116), Expect = 1.5e-121
Identity = 235/436 (53.90%), Postives = 315/436 (72.25%), Query Frame = 0

Query: 127 SEAT-GCEGRSQDEESSSSSSTLMGWPVHQRAAEEGNFDGRELERQ----VSSVSETEM- 186
           SE T G   +  +  S  S ++   WPV   +       G E++      V  +SE E+ 
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNWPVLTESKSSKCLSGLEMQSNECLVVQEISEPELE 106

Query: 187 -MKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLAPERKAMWRRE 246
            MKERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEPL  E++A+W+RE
Sbjct: 107 TMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKRE 166

Query: 247 MEWLLCVSNHIVELIPIWQTFPDGSKLEIMTCRPRSDLCVNLPALRKLDHMLLDLLDSFV 306
           M  LL V ++IVE IP  Q   +G+ +E+M  RPR+D+ +NLPALRKLD ML++ LDSF 
Sbjct: 167 MNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQ 226

Query: 307 DSEFWYVDQGILAAEADGSS--SFRKVL-ERQEDKWWLPIPRVPSGGLSEPSRRQLQHKR 366
           ++EFWY ++G L+ ++  SS  SFRKV+ +R+E+KWWLP+P VPS GLS+ +R+QL++KR
Sbjct: 227 NTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKR 286

Query: 367 DCTNQILKAAMAINSVTLADMDVPISYLEALPKSGRASLGEAIYRYIS-SEEFSPVCLLE 426
           + TNQI KAAMAINS  L++M++P SY+  LPK G++S+G++IYRY+S S  F P  LL+
Sbjct: 287 ESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLD 346

Query: 427 CLDMSSEHQAIEIANRVEAAMYAWRKGTTSKPTNHNSSRSTSKSSWEMLKELVID---AD 486
           CL++SSEH+A+++A+RVEA+MY WR+          S  S SK+SW M+K+L+      D
Sbjct: 347 CLNISSEHEAVQLADRVEASMYTWRR---------KSCLSNSKNSWNMVKDLMSTTERTD 406

Query: 487 KTQLFAERAESLLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLESLAFNIVT 546
           K  + AERAE+LLFCLKQ++P L QTSLD+ KIQ+NKDVGKA+LESYSRVLE LAFNIV 
Sbjct: 407 KNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVA 466

Query: 547 RIDDLLYVDDLTKHSD 549
            IDD+LYVD   + S+
Sbjct: 467 WIDDVLYVDKTMRGSE 473