Lcy06g011200 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g011200
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr06: 12998236 .. 12998730 (+)
RNA-Seq ExpressionLcy06g011200
SyntenyLcy06g011200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACTCAAGCAAAGCATCATGGAGACTGTGATCGATCGAGAGTTGCTCGTAACTCCCACCCAACGGAGTGGACATCGCCGCCTCCCAACCATTTCAAGCTAAACATGGATGCGTCCTTAGCACATGGCGAGATGAGAACATGCATGATCATCAGAAACTCAAAGGGTGAAACAATGGCAGCTATAGAAAATTTTAGGAGAACGAGTTGCTCTGTAGGGTGGGCAGAAGCACAAGCGCTTATGGAGGGTGTGCAACTAGCCTTGGATTCGGGCTTATCACCAGTCTGGGCAGAGATAGATTCCAAAATTGTGTGGAATTTACTCCATGACCATGAACAACATTTAAATGAGATTGCCCCCCTAATTCACCAACTCAATTTTTTGGCCACAAAGCATCACATAAGGGGATTTCTTCTAACTCCGAGGGAAGGTAACAAAGTAGCACACCGGTTAGCTACTCATGCTCGTACAACAAAACACTCTGAAGTCTGA

mRNA sequence

ATGGAGACTCAAGCAAAGCATCATGGAGACTGTGATCGATCGAGAGTTGCTCGTAACTCCCACCCAACGGAGTGGACATCGCCGCCTCCCAACCATTTCAAGCTAAACATGGATGCGTCCTTAGCACATGGCGAGATGAGAACATGCATGATCATCAGAAACTCAAAGGGTGAAACAATGGCAGCTATAGAAAATTTTAGGAGAACGAGTTGCTCTGTAGGGTGGGCAGAAGCACAAGCGCTTATGGAGGGTGTGCAACTAGCCTTGGATTCGGGCTTATCACCAGTCTGGGCAGAGATAGATTCCAAAATTGTGTGGAATTTACTCCATGACCATGAACAACATTTAAATGAGATTGCCCCCCTAATTCACCAACTCAATTTTTTGGCCACAAAGCATCACATAAGGGGATTTCTTCTAACTCCGAGGGAAGGTAACAAAGTAGCACACCGGTTAGCTACTCATGCTCGTACAACAAAACACTCTGAAGTCTGA

Coding sequence (CDS)

ATGGAGACTCAAGCAAAGCATCATGGAGACTGTGATCGATCGAGAGTTGCTCGTAACTCCCACCCAACGGAGTGGACATCGCCGCCTCCCAACCATTTCAAGCTAAACATGGATGCGTCCTTAGCACATGGCGAGATGAGAACATGCATGATCATCAGAAACTCAAAGGGTGAAACAATGGCAGCTATAGAAAATTTTAGGAGAACGAGTTGCTCTGTAGGGTGGGCAGAAGCACAAGCGCTTATGGAGGGTGTGCAACTAGCCTTGGATTCGGGCTTATCACCAGTCTGGGCAGAGATAGATTCCAAAATTGTGTGGAATTTACTCCATGACCATGAACAACATTTAAATGAGATTGCCCCCCTAATTCACCAACTCAATTTTTTGGCCACAAAGCATCACATAAGGGGATTTCTTCTAACTCCGAGGGAAGGTAACAAAGTAGCACACCGGTTAGCTACTCATGCTCGTACAACAAAACACTCTGAAGTCTGA

Protein sequence

METQAKHHGDCDRSRVARNSHPTEWTSPPPNHFKLNMDASLAHGEMRTCMIIRNSKGETMAAIENFRRTSCSVGWAEAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIRGFLLTPREGNKVAHRLATHARTTKHSEV
Homology
BLAST of Lcy06g011200 vs. ExPASy TrEMBL
Match: A0A5C7H0P0 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.3e-12
Identity = 52/139 (37.41%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 20  SHPTEWTSPPPNHFKLNMDAS--LAHGEMRTCMIIRNSKGETMAAIENFRRTSCSVGWAE 79
           SH   W +P    FK+N DAS  L  G+    +IIR+ KG  +AA  +      SV   E
Sbjct: 268 SHQPSWKTPKRGEFKINCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLE 327

Query: 80  AQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIRG 139
           AQA +EG+ LA+D G+S V  E D+  V  LL D      E+  +IH    L    ++  
Sbjct: 328 AQACLEGIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLLS 387

Query: 140 FLLTPREGNKVAHRLATHA 157
           ++   RE N VAH +A HA
Sbjct: 388 YVAVRREANSVAHCIAQHA 406

BLAST of Lcy06g011200 vs. ExPASy TrEMBL
Match: A0A7J9D462 (Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_021255 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.6e-11
Identity = 54/147 (36.73%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 13  RSRVARNSHP-----TEWTSPPPNHFKLNMDA--SLAHGEMRTCMIIRNSKGETMAAIEN 72
           RS      HP      +W+ PP   FK+N+DA  S+A+  + T  IIRN +G  M +   
Sbjct: 152 RSSAESLKHPQPRSMVKWSPPPQGWFKVNVDAGVSIANNRVVTDFIIRNDEGFIMGSGFQ 211

Query: 73  FRRTSCSVGWAEAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQ 132
                 SV  AEA A++ G+Q ALD G S V  E DS++V N +    +  +E  P    
Sbjct: 212 RHNLVLSVVIAEAIAVLHGLQFALDMGFSRVILESDSRLVVNNIQKSSEDYSESRPFTWD 271

Query: 133 LNFLATKHHIRGFLLTPREGNKVAHRL 153
           +  LA K H   F    REGN  AH L
Sbjct: 272 VKNLARKFHCCRFQFVAREGNGAAHAL 298

BLAST of Lcy06g011200 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.8e-10
Identity = 47/140 (33.57%), Postives = 68/140 (48.57%), Query Frame = 0

Query: 19  NSHPTEWTSPPPNHFKLNMDASLAHGEMRTCM--IIRNSKGETMAAIENFRRTSCSVGWA 78
           N+    W  P    +K+N DAS    +    +  IIRN +G+ MA+   +     SV  A
Sbjct: 28  NTAEVLWLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMA 87

Query: 79  EAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIR 138
           EA   +EG+QLA   G++PV  E DS  ++NL     + L+E   ++ +     T+    
Sbjct: 88  EAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHA 147

Query: 139 GFLLTPREGNKVAHRLATHA 157
            F    REGNK AH LA  A
Sbjct: 148 SFNFVKREGNKAAHMLARRA 167

BLAST of Lcy06g011200 vs. ExPASy TrEMBL
Match: A0A0D2MPE5 (RNase H domain-containing protein OS=Gossypium raimondii OX=29730 GN=B456_003G154500 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.6e-09
Identity = 51/147 (34.69%), Postives = 71/147 (48.30%), Query Frame = 0

Query: 13  RSRVARNSHP-----TEWTSPPPNHFKLNMDASLAHGEMRTCM--IIRNSKGETMAAIEN 72
           RS      HP      +W+ PP  +FK+N+DA L+    R  +  IIRN++G  M     
Sbjct: 61  RSSAESLKHPQPRSMVKWSPPPQGYFKINVDAGLSVANNRAVLGFIIRNAEGFIMG---- 120

Query: 73  FRRTSCSVGWAEAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQ 132
                 S+  AEA A+++G+Q ALD G S V  E DS++V N +    +  +E  P    
Sbjct: 121 -HNLVHSMVIAEAIAVLDGLQFALDMGFSKVILESDSRLVVNNIQKSSEDYSESRPFTWD 180

Query: 133 LNFLATKHHIRGFLLTPREGNKVAHRL 153
           +  LA K     F    REGN  AH L
Sbjct: 181 VKNLARKFLCCRFQFVAREGNGAAHAL 202

BLAST of Lcy06g011200 vs. ExPASy TrEMBL
Match: A0A2N9EU90 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.6e-09
Identity = 47/142 (33.10%), Postives = 70/142 (49.30%), Query Frame = 0

Query: 25  WTSPPPNHFKLNMDASL--AHGEMRTCMIIRNSKGETMAAIENFRRTSCSVGWAEAQALM 84
           W+ P P  +K+N DA+      E+   +IIRN  GE MA++ +      SV   EA A  
Sbjct: 509 WSPPQPGRYKVNYDAAFFGDSNEVGIGVIIRNHNGEVMASLCHRIHYPHSVEAMEAYAAR 568

Query: 85  EGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIRGFLLTP 144
             +Q+A+D GL  +  E DS+ V + L +H         LI+  N      H   FL   
Sbjct: 569 SAIQMAIDLGLQAIDIEGDSQTVVDALLNHAPCFTLYGNLINDTNIATQSCHFVQFLHVK 628

Query: 145 REGNKVAHRLATHARTTKHSEV 165
           R+GN +AH +A  A+ ++  EV
Sbjct: 629 RDGNSIAHSIAKRAKFSQPFEV 650

BLAST of Lcy06g011200 vs. NCBI nr
Match: KAG6608327.1 (hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 88.2 bits (217), Expect = 7.2e-14
Identity = 53/142 (37.32%), Postives = 75/142 (52.82%), Query Frame = 0

Query: 23  TEWTSPPPNHFKLNMDASLAHGEMRTCMIIRNSKGETMAAIENFRRTSCSVGWAEAQALM 82
           + W S P +  KL++DA++ + EM          G  M   E        +  A  +   
Sbjct: 20  SRWVSLPHDQDKLSVDATMHNEEM----------GCQMGLFE--------IPKALGKDSS 79

Query: 83  EGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIRGFLLTP 142
            G QL + +GL+ VW +++SK+VW LL D +QH  EIA LI  L  L+  H I+GFL TP
Sbjct: 80  RGNQLGMATGLTSVWPKVNSKVVWTLLMDQDQHFIEIAHLIKHLKHLSQNHCIKGFLPTP 139

Query: 143 REGNKVAHRLATHARTTKHSEV 165
           ++  K  H LA HA  TKH E+
Sbjct: 140 KDDTKATHTLAIHAWHTKHLEI 143

BLAST of Lcy06g011200 vs. NCBI nr
Match: TXG50387.1 (hypothetical protein EZV62_022911 [Acer yangbiense])

HSP 1 Score: 81.6 bits (200), Expect = 6.7e-12
Identity = 52/139 (37.41%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 20  SHPTEWTSPPPNHFKLNMDAS--LAHGEMRTCMIIRNSKGETMAAIENFRRTSCSVGWAE 79
           SH   W +P    FK+N DAS  L  G+    +IIR+ KG  +AA  +      SV   E
Sbjct: 268 SHQPSWKTPKRGEFKINCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLE 327

Query: 80  AQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIRG 139
           AQA +EG+ LA+D G+S V  E D+  V  LL D      E+  +IH    L    ++  
Sbjct: 328 AQACLEGIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLLS 387

Query: 140 FLLTPREGNKVAHRLATHA 157
           ++   RE N VAH +A HA
Sbjct: 388 YVAVRREANSVAHCIAQHA 406

BLAST of Lcy06g011200 vs. NCBI nr
Match: MBA0755499.1 (hypothetical protein [Gossypium gossypioides])

HSP 1 Score: 79.3 bits (194), Expect = 3.3e-11
Identity = 54/147 (36.73%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 13  RSRVARNSHP-----TEWTSPPPNHFKLNMDA--SLAHGEMRTCMIIRNSKGETMAAIEN 72
           RS      HP      +W+ PP   FK+N+DA  S+A+  + T  IIRN +G  M +   
Sbjct: 152 RSSAESLKHPQPRSMVKWSPPPQGWFKVNVDAGVSIANNRVVTDFIIRNDEGFIMGSGFQ 211

Query: 73  FRRTSCSVGWAEAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQ 132
                 SV  AEA A++ G+Q ALD G S V  E DS++V N +    +  +E  P    
Sbjct: 212 RHNLVLSVVIAEAIAVLHGLQFALDMGFSRVILESDSRLVVNNIQKSSEDYSESRPFTWD 271

Query: 133 LNFLATKHHIRGFLLTPREGNKVAHRL 153
           +  LA K H   F    REGN  AH L
Sbjct: 272 VKNLARKFHCCRFQFVAREGNGAAHAL 298

BLAST of Lcy06g011200 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 75.9 bits (185), Expect = 3.7e-10
Identity = 47/140 (33.57%), Postives = 68/140 (48.57%), Query Frame = 0

Query: 19  NSHPTEWTSPPPNHFKLNMDASLAHGEMRTCM--IIRNSKGETMAAIENFRRTSCSVGWA 78
           N+    W  P    +K+N DAS    +    +  IIRN +G+ MA+   +     SV  A
Sbjct: 28  NTAEVLWLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMA 87

Query: 79  EAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIR 138
           EA   +EG+QLA   G++PV  E DS  ++NL     + L+E   ++ +     T+    
Sbjct: 88  EAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHA 147

Query: 139 GFLLTPREGNKVAHRLATHA 157
            F    REGNK AH LA  A
Sbjct: 148 SFNFVKREGNKAAHMLARRA 167

BLAST of Lcy06g011200 vs. NCBI nr
Match: XP_039064257.1 (uncharacterized protein LOC120209283 [Hibiscus syriacus])

HSP 1 Score: 75.5 bits (184), Expect = 4.8e-10
Identity = 52/140 (37.14%), Postives = 70/140 (50.00%), Query Frame = 0

Query: 20  SHP----TEWTSPPPNHFKLNMDASLAH--GEMRTCMIIRNSKGETMAAIENFRRTSCSV 79
           +HP    T W  P     K+N DAS +H   E  + +IIR+S GE + A +   R + SV
Sbjct: 110 THPPPKQTRWLPPNAPWVKVNFDASFSHTNKEAWSGIIIRDSVGEILGAAKRKIRRATSV 169

Query: 80  GWAEAQALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKH 139
             AEA A +  +Q  +D G      E DSK +   L   E+ L+E+  +I    FLA+K 
Sbjct: 170 FAAEATAAVHAIQFRIDLGCMRAIFEGDSKTIIQKLQSDEEDLSEVGAMIWDGKFLASKL 229

Query: 140 HIRGFLLTPREGNKVAHRLA 154
           H   F  T R GN  AH LA
Sbjct: 230 HAATFHFTQRAGNVAAHILA 249

BLAST of Lcy06g011200 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.0e-06
Identity = 38/136 (27.94%), Postives = 59/136 (43.38%), Query Frame = 0

Query: 23  TEWTSPPPNHFKLNMDASLAHGE--MRTCMIIRNSKGE-TMAAIENFRRTSCSVGWAEAQ 82
           ++W  PP    K N D+    G    R+   IR   G   +      + ++CS+  AEA 
Sbjct: 204 SQWNPPPEGWVKCNFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSL-HAEAL 263

Query: 83  ALMEGVQLALDSGLSPVWAEIDSKIVWNLLHDHEQHLNEIAPLIHQLNFLATKHHIRGFL 142
             +  +Q+    GL  VW E DSK +  L+++ E H + +  LI+ +     K       
Sbjct: 264 GFLHALQVIWAHGLRYVWFESDSKSLVTLINNGEDH-SLLGTLIYDIRHWMLKLPYCSLE 323

Query: 143 LTPREGNKVAHRLATH 156
              RE N  A  LA+H
Sbjct: 324 FVNRERNSAADALASH 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5C7H0P03.3e-1237.41Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1[more]
A0A7J9D4621.6e-1136.73Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_021255 PE=4 ... [more]
A0A6J1CIF11.8e-1033.57uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A0D2MPE52.6e-0934.69RNase H domain-containing protein OS=Gossypium raimondii OX=29730 GN=B456_003G15... [more]
A0A2N9EU902.6e-0933.10Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAG6608327.17.2e-1437.32hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sorori... [more]
TXG50387.16.7e-1237.41hypothetical protein EZV62_022911 [Acer yangbiense][more]
MBA0755499.13.3e-1136.73hypothetical protein [Gossypium gossypioides][more]
XP_022140628.13.7e-1033.57uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_039064257.14.8e-1037.14uncharacterized protein LOC120209283 [Hibiscus syriacus][more]
Match NameE-valueIdentityDescription
AT2G02650.12.0e-0627.94Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 31..157
e-value: 1.2E-10
score: 43.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 48..156
e-value: 2.9E-21
score: 75.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 14..157
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 51..153
e-value: 1.0621E-16
score: 69.6504
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 32..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g011200.1Lcy06g011200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity