Lcy06g010090 (gene) Sponge gourd (P93075) v1

BLAST of Lcy06g010090 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 6.0e-153
Identity = 263/517 (50.87%), Postives = 356/517 (68.86%), Query Frame = 0

Query: 27  SNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLIIITP 86
           +N  ++F +C   H P++ +  K+VYT  +  Y S+LN +I+NLRF +  TPKPL+I+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 87  SNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVAYLPFIVVDLINLRSITVDVENSTA 146
           SN SHIQA ++CSK   LQIR RSGGHD EG+SY++ +PF+VVDL N+ SI +DV + TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 147 WVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY I EK+  L+FP G CPTVGVGGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 YFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPTTVTFCSTNRTWE-E 266
           + V+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 267 GAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG-GKINPVALFFSLFLGKADELVGI 326
           G +KL ++WQ +A K +++L L       N++   G  K      F S+F G  D LV +
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 327

Query: 327 LKTTFPELGLTKEDCIETTWIESTLIAAN--GFQNGGAALEALLSRTPPTNGSTKIKSDY 386
           +  +FPELG+ K DC E +WI++T+  +    F       E LL R+     +  IK DY
Sbjct: 328 MNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDY 387

Query: 387 VKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQISESEIPFSHRAGNLYKIGYVV 446
           VK+PI + A+  I E+L  +D+ A   +  PYGG M +ISES IPF HRAG +Y++ Y  
Sbjct: 388 VKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTA 447

Query: 447 GGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYERARV 506
             ++Q  +  +K++ W+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR+
Sbjct: 448 SWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARI 507

Query: 507 WGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTLSP 540
           WG KYFG NFNRLV VKTKVDP NFFR+EQSIP L P
Sbjct: 508 WGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of Lcy06g010090 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.8e-149
Identity = 254/517 (49.13%), Postives = 354/517 (68.47%), Query Frame = 0

Query: 27  SNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLIIITP 86
           +N  ++F +C  ++ P++ +  K +YT  +  Y SVLN +I+NLRF++  TPKPL+I+TP
Sbjct: 28  ANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTP 87

Query: 87  SNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVAYLPFIVVDLINLRSITVDVENSTA 146
           SN+SHIQA+++CSK   LQIR RSGGHD EGLSY++ +PF +VDL N+ ++ VD+ + TA
Sbjct: 88  SNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTA 147

Query: 147 WVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY I E +   +FP G CPTVGVGGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 YFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPTTVTFCSTNRTWE-E 266
           + V+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIH 267

Query: 267 GAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG-GKINPVALFFSLFLGKADELVGI 326
           G +KL ++WQ +A K +++L L       N++   G  K      F S+FLG  D LV +
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDL 327

Query: 327 LKTTFPELGLTKEDCIETTWIESTLIAAN--GFQNGGAALEALLSRTPPTNGSTKIKSDY 386
           +  +FPELG+ K DC E +WI++T+  +    +       E LL R+     +  IK DY
Sbjct: 328 MNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDY 387

Query: 387 VKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQISESEIPFSHRAGNLYKIGYVV 446
           VK+ I + A+  I E+L  +++     +  PYGG M +ISES IPF HRAG +Y++ Y  
Sbjct: 388 VKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTA 447

Query: 447 GGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYERARV 506
             ++Q  +  +K++ W+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR+
Sbjct: 448 TWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARI 507

Query: 507 WGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTLSP 540
           WG KYFG NFNRLV VKTK DP NFFR+EQSIP L P
Sbjct: 508 WGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of Lcy06g010090 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.0e-149
Identity = 257/516 (49.81%), Postives = 353/516 (68.41%), Query Frame = 0

Query: 27  SNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLIIITP 86
           +N  ++F +C   + P + +  K+VYT  +  Y S+LN +I+NLRF++  TPKPL+IITP
Sbjct: 28  ANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITP 87

Query: 87  SNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVAYLPFIVVDLINLRSITVDVENSTA 146
            N+SHIQ  ++CSK   LQIR RSGGHD EG+SY++ +PF++VDL N+ S+ +DV + TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 147 WVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 YFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPTTVTFCSTNRTWE-E 266
           + V+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 267 GAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG-GKINPVALFFSLFLGKADELVGI 326
             +KL+++WQ +A    + L L       N++   G  K    + F S+F G  D LV +
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDL 327

Query: 327 LKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAAL---EALLSRTPPTNGSTKIKSD 386
           +  +FPELG+ K DC + +WI+ T+I  +G  N        E LL R+     +  IK D
Sbjct: 328 MNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLD 387

Query: 387 YVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQISESEIPFSHRAGNLYKIGYV 446
           YVK+PI + A+  I E+L  +D+     +F PYGG M +ISES IPF HRAG +Y+I Y+
Sbjct: 388 YVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYI 447

Query: 447 VGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYERAR 506
              ++Q  +  +K++ WIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR
Sbjct: 448 ASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQAR 507

Query: 507 VWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           +WG KYFG NFNRLV VKTKVDP NFFR+EQSIP L
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Lcy06g010090 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 256/514 (49.81%), Postives = 353/514 (68.68%), Query Frame = 0

Query: 27  SNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLIIITP 86
           +N  ++F +C   + P++ +  K+VYT  N  Y SVLN +I NLRF++  TPKPL+I+TP
Sbjct: 28  ANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTP 87

Query: 87  SNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVAYLPFIVVDLINLRSITVDVENSTA 146
           S++SHIQ  ++CSK   LQIR RSGGHD EG+SY++ +PF++VDL N+RSI +DV + TA
Sbjct: 88  SHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTA 147

Query: 147 WVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY + EK+  L+  AG CPTV  GGHF GGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDA 207

Query: 207 YFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPTTVTFCSTNRTWEEG 266
           + V+ +GK  DR+SMGEDLFWA+RGGG  SFGI+VAWK++LV VP +  F          
Sbjct: 208 HLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKIMEIHE 267

Query: 267 AIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTGGKINPVALFF-SLFLGKADELVGIL 326
            +KL+++WQ +A K +++L L       N++   G     +  +F S+FLG  D LV ++
Sbjct: 268 LVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLM 327

Query: 327 KTTFPELGLTKEDCIETTWIESTLIAAN--GFQNGGAALEALLSRTPPTNGSTKIKSDYV 386
             +FPELG+ K DC + +WI++ +  +    +       E LL R+   NG+ KIK DYV
Sbjct: 328 NKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYV 387

Query: 387 KEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQISESEIPFSHRAGNLYKIGYVVG 446
           K+PI ++    I E+L  +DI A      PYGG M +ISES IPF HRAG LY++ Y+  
Sbjct: 388 KKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICS 447

Query: 447 GKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYERARVW 506
            ++Q  +  +K+L WIR +Y++MTP+VSK+PR AY+NYRDLDIG N+     +Y +AR+W
Sbjct: 448 WEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIW 507

Query: 507 GLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           G KYFG NF+RLV VKT VDP NFFR+EQSIP L
Sbjct: 508 GEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Lcy06g010090 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 257/516 (49.81%), Postives = 352/516 (68.22%), Query Frame = 0

Query: 27  SNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLIIITP 86
           +N  ++F +CL  + P + +  K+VYT  +  Y S+LN +++NLRF++  TPKPL+I TP
Sbjct: 28  ANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTP 87

Query: 87  SNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVAYLPFIVVDLINLRSITVDVENSTA 146
            N+SHIQ  ++CSK   LQIR RSGGHD EG+SY++ +PF++VDL N+ S+ +DV + TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 147 WVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV+SGATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 YFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPTTVTFCSTNRTWE-E 266
           + V+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 267 GAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG-GKINPVALFFSLFLGKADELVGI 326
             +KL+++WQ +A    + L L       N++   G  K    + F S+F G  D LV +
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDL 327

Query: 327 LKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAA---LEALLSRTPPTNGSTKIKSD 386
           +  +FPELG+ K DC + +WI+ T+I  +G  N        E LL R+     +  IK D
Sbjct: 328 MNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLD 387

Query: 387 YVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQISESEIPFSHRAGNLYKIGYV 446
           YVK+PI + A+  I E+L  +D+     +F PYGG M +ISES IPF HRAG  Y+I Y+
Sbjct: 388 YVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYI 447

Query: 447 VGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYERAR 506
              ++Q  +  +K++ WIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR
Sbjct: 448 ASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQAR 507

Query: 507 VWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           +WG KYFG NFNRLV VKTKVDP NFFR+EQSIP L
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Lcy06g010090 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 5.1e-248
Identity = 428/540 (79.26%), Postives = 477/540 (88.33%), Query Frame = 0

Query: 3   SSSLILLSFA--FIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDS-YSITKVVYTPINSSY 62
           SS LI LSF+  FI LSSSSLW   S +KH+ F  CLLDHSPDS YSI+KVVYTP+NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLS 122
           SSVL+FSIRNLRFST  TPKPL IITP ++SH+QAAV+CS++H LQIR RSGGHD EGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVG 182
            V++LPFI++DLINLRSIT+D  N+TAWVQSGATLGELYY IAEKSRTLAFPAG CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGI 242
           +GGHFSGGGYGLMLRK+GLAADNVIDAY VDANGKF DRE MGEDLFWAIRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQ 302
           VVAWKVKLVPVP TVT C  NRT EEGAIKLI +WQYVANKL+ENLFLGILL GGN + Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 TGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGG 362
            G K NP A FFSLFLGKADE   IL TTFPELGL KE+C+E++WIES L++A GF+ G 
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGD 363

Query: 363 AALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMS 422
            +LEALL+RTP TNGS KIKSDYVKEPI++A IEGIWERLKS+D+ A  L+FVPYGGRMS
Sbjct: 364 -SLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMS 423

Query: 423 QISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYV 482
           QISESEIPFSHRAGNLYKIGY+V  ++Q++D EK++LKWIRELYSYMTPFVSKSPR+AYV
Sbjct: 424 QISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYV 483

Query: 483 NYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTLSP 540
           NYRDLDIGSNNKYGKTS++RA VWG KYFGNNFNRLVHVKTKVDPY+FFRHEQSIPT+SP
Sbjct: 484 NYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTISP 541

BLAST of Lcy06g010090 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.2e-244
Identity = 416/536 (77.61%), Postives = 473/536 (88.25%), Query Frame = 0

Query: 4   SSLILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPD--SYSITKVVYTPINSSYSS 63
           S LI LS AFI LSSSSLW     NKH+ F +CLLDHS +  +YSI+KV++TPINSSYSS
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 64  VLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYV 123
           VL+FSIRNLRFST +TPKPL+I+TPS++SHIQAAVICSKS+  QIR RSGGHD EGLSYV
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 124 AYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVG 183
           A+LPFI+VDLINLRSI++D++++TAWVQSGATLGELYY IA+KSRTLAFPAG CPTVGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 184 GHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 243
           GHFSGGGYGL+LRKYGLAADNVIDAY VDA+GKF++RESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG 303
           AWKVKLVPVP TVT CS NRT EEGA+K+  +WQYVANKL+ENLFLGIL  GG +++Q G
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 304 GKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAA 363
           GK NPVA FFSLFLGKADEL+ IL T FPEL L K+DCIET+WIEST++   GF      
Sbjct: 305 GKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT-ELT 364

Query: 364 LEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQI 423
           LEALLSRTP TN S+KIKSDYVKEP+++A ++GIW+RL SQDIE  N+IFVPYGGRMSQI
Sbjct: 365 LEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQI 424

Query: 424 SESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNY 483
           SESE PFSHRAG LYKIGY+ G +EQ +DAEK++L+WIRELY YMTPFVSKSPRAAY NY
Sbjct: 425 SESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANY 484

Query: 484 RDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           RDLDIGSNNK+GKTSY++A VWGLKYFGNNFNRLVHVKTKVDPY+FFRHEQSIP L
Sbjct: 485 RDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Lcy06g010090 vs. ExPASy TrEMBL
Match: A0A6J1KJP1 (berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 8.5e-243
Identity = 416/544 (76.47%), Postives = 478/544 (87.87%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYS 60
           MKSS L LLS  FIVL SSSLW   SSN+++DF +CLL HSPD++SITK+VYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSH LQIRIRSGGHDLEGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 VAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGV 180
           VAY+PFIVVDLINL  + VDVE +TAWVQSGA+LGELYY+IAEKSRTLAFPAG CPTVGV
Sbjct: 121 VAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240
           GG FSGGGYG+++RKYGLAADNV+DAY VDANG+F+DRE MGE+LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQT 300
           VAWKVKLVPVP TVT C+T+++  +GAI LID+WQYVA+KL+ENLFLGI+L G       
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------- 300

Query: 301 GGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGA 360
           GGK NP+A+FFSLFLGKADELV IL   FPELGL+KEDCIET+WIE+ LIAANGFQ  G 
Sbjct: 301 GGKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQT-GE 360

Query: 361 ALEALLSRT---PPTN-GSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGG 420
            LEALL+R+   PP N G +K KSDYVKEP+T+ AIEG+W R KSQ+IE A+L+F+PYGG
Sbjct: 361 VLEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGG 420

Query: 421 RMSQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRA 480
           +MS+ISESEIPF HRAGN Y+I Y+VG  EQ + AEK++L WIRELYSYMTPFVSKSPRA
Sbjct: 421 KMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPT 540
            YVN RDLDIGSN+ YG+TSY+RARVWGLKYFGNNF+RLVHVKT+VDPY+FFRHEQSIPT
Sbjct: 481 VYVNCRDLDIGSNDMYGRTSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQSIPT 536

BLAST of Lcy06g010090 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.9e-242
Identity = 417/539 (77.37%), Postives = 471/539 (87.38%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVL-SSSSLWTVVSSN-KHQDFFRCLLDHSPDSYSITKVVYTPINSS 60
           MK S L+ LS AFIV+ SSSS+W   SS+  H++F  CLL HSPDS SITK+VYTPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGL 120
           YS VLNFSIRNLRFSTP TPKPL+IITPSNISH+QAAVICSKSH +QIRIRSGGHD EGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTV 180
           SYVAY PF+VVDLINL S+T++VE STAWVQSGATLGELYY+IAEKSRTLAFPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G +LRKYGLAADNVIDAY VDANG+F+DR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVST 300
           IVVAWKVKLVPVP TVT C+ +RT EE AIKLIDQWQYVANKL+E+LFLGI L GG +  
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QTGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNG 360
           Q G KINP+ALFFSLFLGKADEL+ IL  TFP+LGLTKE+C ET+WIES +   NG Q  
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 GAALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRM 420
              LE LL+RTP  NG+ K+KSDYVKEPI+KA I+ +W+RLKSQDIE ANL+F+PYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 SQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAY 480
           SQISESEIPFSHRAGNLYKIGY+ G  E  ++AEK +L WIR++YSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           VNYRDLDIGSN+KYGKTSY+RA VWG KYFG+NF+RLV+VK +VDPYNFFRHEQSIP L
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVL 538

BLAST of Lcy06g010090 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.9e-242
Identity = 417/539 (77.37%), Postives = 471/539 (87.38%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVL-SSSSLWTVVSSN-KHQDFFRCLLDHSPDSYSITKVVYTPINSS 60
           MK S L+ LS AFIV+ SSSS+W   SS+  H++F  CLL HSPDS SITK+VYTPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGL 120
           YS VLNFSIRNLRFSTP TPKPL+IITPSNISH+QAAVICSKSH +QIRIRSGGHD EGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTV 180
           SYVAY PF+VVDLINL S+T++VE STAWVQSGATLGELYY+IAEKSRTLAFPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G +LRKYGLAADNVIDAY VDANG+F+DR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVST 300
           IVVAWKVKLVPVP TVT C+ +RT EE AIKLIDQWQYVANKL+E+LFLGI L GG +  
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QTGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNG 360
           Q G KINP+ALFFSLFLGKADEL+ IL  TFP+LGLTKE+C ET+WIES +   NG Q  
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 GAALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRM 420
              LE LL+RTP  NG+ K+KSDYVKEPI+KA I+ +W+RLKSQDIE ANL+F+PYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 SQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAY 480
           SQISESEIPFSHRAGNLYKIGY+ G  E  ++AEK +L WIR++YSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           VNYRDLDIGSN+KYGKTSY+RA VWG KYFG+NF+RLV+VK +VDPYNFFRHEQSIP L
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVL 538

BLAST of Lcy06g010090 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 872.8 bits (2254), Expect = 1.5e-249
Identity = 431/539 (79.96%), Postives = 475/539 (88.13%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVL-SSSSLWTVVSSN--KHQDFFRCLLDHSPDSYSITKVVYTPINS 60
           MK S LI LS AFI+L SSSSL +  SS+  K+++F +CLL HSP++YSIT +VY P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SYSSVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEG 120
           SYSS+LNFSIRNLRFSTP  PKPL+IITPSNISHIQAAV+CSKSH LQIRIRSGGHD EG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPT 180
           LSYVAY PFIVVDLINL S+ VDVE STAWVQSGATLGELYY+IAEKSRTLAFPAGTCPT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG +LRKYGLAADNVIDAY VDANG+F+DRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVS 300
           GIVVAWKVKLVPVP TVT C+ NRTW+EGAIKLIDQWQYVANKL+E+LFL I+LTGG +S
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 TQTGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQN 360
            Q GGKINP ALFF+LFLGKADEL+ ILK  FPEL L KEDC ET+WIES +  ANG Q 
Sbjct: 301 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 361 GGAALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGR 420
               LEA L+RTP  NGS KIKSDYVKEPITKAAIEGIW+RLKSQDIE  NL  +PYGGR
Sbjct: 361 DDQPLEAFLNRTPLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGR 420

Query: 421 MSQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAA 480
           MSQISESEIPF HR GNLYKIGY++G +EQ +DAEK++L WIRE+YSYMTPFVSKSPRAA
Sbjct: 421 MSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPT 537
           YVNYRDLDIGSN+KYGKTSY+RA +WG KYFGNNFNRLVHVK +VDPY+FFRHEQSIP+
Sbjct: 481 YVNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSIPS 538

BLAST of Lcy06g010090 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 866.7 bits (2238), Expect = 1.1e-247
Identity = 428/540 (79.26%), Postives = 477/540 (88.33%), Query Frame = 0

Query: 3   SSSLILLSFA--FIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDS-YSITKVVYTPINSSY 62
           SS LI LSF+  FI LSSSSLW   S +KH+ F  CLLDHSPDS YSI+KVVYTP+NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLS 122
           SSVL+FSIRNLRFST  TPKPL IITP ++SH+QAAV+CS++H LQIR RSGGHD EGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVG 182
            V++LPFI++DLINLRSIT+D  N+TAWVQSGATLGELYY IAEKSRTLAFPAG CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGI 242
           +GGHFSGGGYGLMLRK+GLAADNVIDAY VDANGKF DRE MGEDLFWAIRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQ 302
           VVAWKVKLVPVP TVT C  NRT EEGAIKLI +WQYVANKL+ENLFLGILL GGN + Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 TGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGG 362
            G K NP A FFSLFLGKADE   IL TTFPELGL KE+C+E++WIES L++A GF+ G 
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGD 363

Query: 363 AALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMS 422
            +LEALL+RTP TNGS KIKSDYVKEPI++A IEGIWERLKS+D+ A  L+FVPYGGRMS
Sbjct: 364 -SLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMS 423

Query: 423 QISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYV 482
           QISESEIPFSHRAGNLYKIGY+V  ++Q++D EK++LKWIRELYSYMTPFVSKSPR+AYV
Sbjct: 424 QISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYV 483

Query: 483 NYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTLSP 540
           NYRDLDIGSNNKYGKTS++RA VWG KYFGNNFNRLVHVKTKVDPY+FFRHEQSIPT+SP
Sbjct: 484 NYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTISP 541

BLAST of Lcy06g010090 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 855.5 bits (2209), Expect = 2.4e-244
Identity = 416/536 (77.61%), Postives = 473/536 (88.25%), Query Frame = 0

Query: 4   SSLILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPD--SYSITKVVYTPINSSYSS 63
           S LI LS AFI LSSSSLW     NKH+ F +CLLDHS +  +YSI+KV++TPINSSYSS
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 64  VLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYV 123
           VL+FSIRNLRFST +TPKPL+I+TPS++SHIQAAVICSKS+  QIR RSGGHD EGLSYV
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 124 AYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVG 183
           A+LPFI+VDLINLRSI++D++++TAWVQSGATLGELYY IA+KSRTLAFPAG CPTVGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 184 GHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 243
           GHFSGGGYGL+LRKYGLAADNVIDAY VDA+GKF++RESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG 303
           AWKVKLVPVP TVT CS NRT EEGA+K+  +WQYVANKL+ENLFLGIL  GG +++Q G
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 304 GKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAA 363
           GK NPVA FFSLFLGKADEL+ IL T FPEL L K+DCIET+WIEST++   GF      
Sbjct: 305 GKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT-ELT 364

Query: 364 LEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQI 423
           LEALLSRTP TN S+KIKSDYVKEP+++A ++GIW+RL SQDIE  N+IFVPYGGRMSQI
Sbjct: 365 LEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQI 424

Query: 424 SESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNY 483
           SESE PFSHRAG LYKIGY+ G +EQ +DAEK++L+WIRELY YMTPFVSKSPRAAY NY
Sbjct: 425 SESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANY 484

Query: 484 RDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           RDLDIGSNNK+GKTSY++A VWGLKYFGNNFNRLVHVKTKVDPY+FFRHEQSIP L
Sbjct: 485 RDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Lcy06g010090 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 853.6 bits (2204), Expect = 9.3e-244
Identity = 424/543 (78.08%), Postives = 468/543 (86.19%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVL-SSSSLWTVVSSN--KHQDFFRCLLDHSPDSYSITKVVYTPINS 60
           MK S LILLS AFI++ SSSSL +  SS+  KH++F +CLL HSP++YSITK+VY PINS
Sbjct: 1   MKFSPLILLSLAFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 60

Query: 61  SYSSVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEG 120
           SYSS+LNF  RN RFSTP  P PL+IITP+NISHIQAA+ICSKSH LQIRIRSGGHD EG
Sbjct: 61  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPT 180
           LSYVAY+PFIVVDLINLRS+TVD E S+AWVQSGATLGELYY+IAEKS+TLAFPAG CPT
Sbjct: 121 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG++LRKYGLAADNVIDAY VDANG+F+DRESMGEDLFWAIRGGGGG+F
Sbjct: 181 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 240

Query: 241 GIVVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVS 300
           G+VVAWKVKLVPVP TVT C+  RT EEGAI+LIDQWQYV NKL E+LFL I L GG  S
Sbjct: 241 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 300

Query: 301 TQTGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQN 360
            Q GGK+NP ALFFSLFLGKADELV ILK  FPELGLTKEDC E +WI S + AAN FQ 
Sbjct: 301 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 360

Query: 361 GGAALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGR 420
               LEALL+RTP  NGS KIKSDYVKE ITK  IEGIWERLKSQDIE   L  +PYGGR
Sbjct: 361 EDQPLEALLNRTPLANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGGR 420

Query: 421 MSQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAA 480
           M QIS+SEIPFSHRAG  Y+IGY+VG KEQ +DAEK+++ WIRE+YSYMTPFVSKSPRAA
Sbjct: 421 MGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 540
           YVNYRD DIGSNNKYGK SY+RARVWGLKYFGNNFNRLV +KTKVDP NFFRHEQSIP L
Sbjct: 481 YVNYRDHDIGSNNKYGKVSYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFRHEQSIP-L 540

BLAST of Lcy06g010090 vs. NCBI nr
Match: XP_023000935.1 (berberine bridge enzyme-like 27 [Cucurbita maxima])

HSP 1 Score: 849.4 bits (2193), Expect = 1.8e-242
Identity = 416/544 (76.47%), Postives = 478/544 (87.87%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYS 60
           MKSS L LLS  FIVL SSSLW   SSN+++DF +CLL HSPD++SITK+VYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSH LQIRIRSGGHDLEGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 VAYLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGV 180
           VAY+PFIVVDLINL  + VDVE +TAWVQSGA+LGELYY+IAEKSRTLAFPAG CPTVGV
Sbjct: 121 VAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240
           GG FSGGGYG+++RKYGLAADNV+DAY VDANG+F+DRE MGE+LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQT 300
           VAWKVKLVPVP TVT C+T+++  +GAI LID+WQYVA+KL+ENLFLGI+L G       
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------- 300

Query: 301 GGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGA 360
           GGK NP+A+FFSLFLGKADELV IL   FPELGL+KEDCIET+WIE+ LIAANGFQ  G 
Sbjct: 301 GGKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQT-GE 360

Query: 361 ALEALLSRT---PPTN-GSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGG 420
            LEALL+R+   PP N G +K KSDYVKEP+T+ AIEG+W R KSQ+IE A+L+F+PYGG
Sbjct: 361 VLEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGG 420

Query: 421 RMSQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRA 480
           +MS+ISESEIPF HRAGN Y+I Y+VG  EQ + AEK++L WIRELYSYMTPFVSKSPRA
Sbjct: 421 KMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPT 540
            YVN RDLDIGSN+ YG+TSY+RARVWGLKYFGNNF+RLVHVKT+VDPY+FFRHEQSIPT
Sbjct: 481 VYVNCRDLDIGSNDMYGRTSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQSIPT 536

BLAST of Lcy06g010090 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 518.8 bits (1335), Expect = 5.1e-147
Identity = 268/537 (49.91%), Postives = 370/537 (68.90%), Query Frame = 0

Query: 10  SFAF-IVLSSSSLWTVVSS--NKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSVLNFS 69
           SF F I+L S ++  +VS+  + H+DF +CL     D+    KV++T  +SS+ S+L+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 70  IRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVAY-LP 129
           I+N RFS  +TPKP+ IITP   S +Q  + C++ H + +R RS GH  EGLSY+AY  P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 130 FIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGGHFS 189
           F V+DL NLRSI++DV+N T WVQ+GAT GELYY+I + +++LAFPAG  PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 190 GGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKV 249
           GGGYG +LRKYGLAADN+IDA  VDA+G+  DR++MGED FWAIRGGGG SFG++++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 250 KLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTGGKIN 309
           KLV VP+T+T     +T ++ A+++I +WQY A+K+ ++LF+   L   N       K  
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-------KNA 304

Query: 310 PVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAALEAL 369
             ALF  L++G  + L+ +++  FPELGL KE C E +WIES L  A+ F  G +    +
Sbjct: 305 VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESL--GV 364

Query: 370 LSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQISESE 429
           L+    T+ S K K D+V+EPI +AAI+ IW RL++ +     +I  P+GG+MS+++E E
Sbjct: 365 LTNRERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYE 424

Query: 430 IPFSHRAGNLYKIGYVVGGKEQSMDAEK----KYLKWIRELYSYMTPFVSKSPRAAYVNY 489
            PF HR GNLY+I YV   +E+  D  K    KYLKW+  +Y +MTP+VSKSPR AYVN+
Sbjct: 425 TPFPHRGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNF 484

Query: 490 RDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTLS 539
           +D+D+G      KT YE  + WG+KYF NNF RLV VKT+VDP +FF  EQSIP ++
Sbjct: 485 KDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Lcy06g010090 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 3.3e-146
Identity = 271/542 (50.00%), Postives = 362/542 (66.79%), Query Frame = 0

Query: 1   MKSSSLILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYS 60
           MK + +++L F   +  SSS     SS   + F +CL  +S   + I+  ++   N SYS
Sbjct: 1   MKYALILVLFFVVFIWQSSS-----SSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSY 120
           SVL  +IRNLRF+T  TPKP +II  ++ SH+QAA+ C K H+LQ++IRSGGHD +GLSY
Sbjct: 61  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 120

Query: 121 VAY--LPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTV 180
           V Y   PF V+D+ NLRS+ VDV + TAWVQ+GA LGE+YY I EKS+TLA+PAG CPTV
Sbjct: 121 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 180

Query: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYG M+RKYGL  DN IDA  VD NGK  DR+ MGEDL+WAI GGGGGS+G
Sbjct: 181 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 240

Query: 241 IVVAWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILL--TGGNV 300
           +V+A+K+ LV VP  VT    +RT E+ A  +I +WQ VA KL + LF+  ++    G V
Sbjct: 241 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 300

Query: 301 STQTGGKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQ 360
           S+Q   +      F ++FLG    L+ IL   FPELGL + DC ET+WI+S L   N   
Sbjct: 301 SSQKTVR----TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN--I 360

Query: 361 NGGAALEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGG 420
             G++   LL R  P N   K KSDYV+EPI++  +E IW+  K  ++E   + F PYGG
Sbjct: 361 QVGSSETLLLQRNQPVN-YLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGG 420

Query: 421 RMSQISESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRA 480
            M +IS +  PF +RAGNL+KI Y    +++++    +Y++  R+LY +MTPFVSK+PR 
Sbjct: 421 EMGRISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQ 480

Query: 481 AYVNYRDLDIGSNNKYGK-TSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIP 538
           ++ NYRD+D+G N+  GK +SY   + +G KYF  NF RLV +KT+VD  NFFR+EQSIP
Sbjct: 481 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of Lcy06g010090 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 514.6 bits (1324), Expect = 9.5e-146
Identity = 260/535 (48.60%), Postives = 374/535 (69.91%), Query Frame = 0

Query: 6   LILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDSYSITKVVYTPINSS--YSSVL 65
           L+ L    + LSSS+L         QDF +CL+D+S  S+ IT   ++P  ++  +   L
Sbjct: 14  LVTLLLISVPLSSSTL--------QQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEEL 73

Query: 66  NFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVA- 125
             + +NLR+ TP  PKP+ I  P   +H+QAAV+C+K   L +R+RSGGHD EGLS+VA 
Sbjct: 74  ESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAE 133

Query: 126 -YLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVG 185
              PF++VDL  LR + VD+++++AW  +GAT+GE+YY+I EKS+T  FPAG C ++G+G
Sbjct: 134 DETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIG 193

Query: 186 GHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 245
           GH  GG YG M+RK+GL ADNV+DA  VDANG+  DR +MGED+FWAIRGGGGGSFG+++
Sbjct: 194 GHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVIL 253

Query: 246 AWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG 305
           AWK+KLVPVP TVT  +  +T E+   K++ +W+ +A+KL+++LF+ ++++  + +T+ G
Sbjct: 254 AWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG 313

Query: 306 GKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAA 365
            +   ++ + + FLG ++ L+ +++ +FPELGLTK+DC E +WI+S +  A GF N  AA
Sbjct: 314 NRTISMS-YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPN-SAA 373

Query: 366 LEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQI 425
            EALL+         K KSD+VKEPI    +EG+WER   +D  +   I+ PYGG MS+I
Sbjct: 374 PEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRI 433

Query: 426 SESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNY 485
           SESEIPF HR G L+KI ++   ++  + +E++++KWIRE+YSYM  +VSK+PR AYVNY
Sbjct: 434 SESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNY 493

Query: 486 RDLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPT 537
           RDLD+G+N   G+T    AR WG KY+  NF RLV +K + DP NFFRHEQS+PT
Sbjct: 494 RDLDLGTNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Lcy06g010090 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 262/533 (49.16%), Postives = 364/533 (68.29%), Query Frame = 0

Query: 3   SSSLILLSFAFIVLSSSSLWTVVSSNKHQDFFRCLLDHSPDSYSITKVVYTPINSSYSSV 62
           SS LI  + + ++LS     +   SN+   F +CL     DS  ++KV++TP ++S+SSV
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSANRSNQ-SSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 63  LNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVA 122
           L  SI+N RFS P  PKP++I+TP   S +Q+AV C++   + IR RSGGHD EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 123 YLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVGG 182
           + PF+++DL NLRSITVDV+N + WVQ+GAT+GELYY+I +K+RTLAFPAG CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 183 HFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 242
           HFSGGGYG +LRK+GLAAD+VIDA  VDA G+  +R  MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 243 WKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTGG 302
           WK+ L+ VP+TVT  +  +  E+ A+K+I +WQ+VA+K++++LF+ ++L       +   
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVR--- 307

Query: 303 KINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAAL 362
                A F  L+LG    L+ ++   FPELGL ++DC E +WIES +  A   + G   +
Sbjct: 308 -----ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPI 367

Query: 363 EALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQIS 422
             L  RT   + + K KSD+V+EP+ K AI  +W RL+  + E A LIF P+GG+MS+I+
Sbjct: 368 NVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 427

Query: 423 ESEIPFSHRAGNLYKIGYVVGGKEQSMDAEKKYLKWIRELYSYMTPFVSKSPRAAYVNYR 482
           + E PF HR GN+Y+I Y+        D ++KY++W+  +Y  M+ FV+KSPR AY+N R
Sbjct: 428 DYETPFPHRKGNIYEIQYL---NYWRGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 487

Query: 483 DLDIGSNNKYGKTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIP 536
           DLD+G      ++ YE  + WG+KYF NNF RLV VKT VDP +FF  EQSIP
Sbjct: 488 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Lcy06g010090 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.8e-143
Identity = 259/540 (47.96%), Postives = 369/540 (68.33%), Query Frame = 0

Query: 6   LILLSFAFIVLS-SSSLWTVVSSNK--HQDFFRCLLDHSPDSYSITKVVYTPINSSYSSV 65
           L+ L   F++L+ S S +  +S+ +  H++F +CL     +  S  ++++T  + SY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 66

Query: 66  LNFSIRNLRFSTPKTPKPLIIITPSNISHIQAAVICSKSHDLQIRIRSGGHDLEGLSYVA 125
           LN SI+N RF   +TPKP+ IITP   + +Q+ + C++ H + IR RSGGHD EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -YLPFIVVDLINLRSITVDVENSTAWVQSGATLGELYYKIAEKSRTLAFPAGTCPTVGVG 185
              PF+V+DL NLRSIT+DV+N T WVQSGAT+GELYY+I + S++LAFPAG  PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGYGLMLRKYGLAADNVIDAYFVDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 245
           G F GGGYG ++RKYGL+ADNVIDA+ VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPTTVTFCSTNRTWEEGAIKLIDQWQYVANKLNENLFLGILLTGGNVSTQTG 305
           +WK++L+ VP+ VT     +T E+ A+ +I++WQY+A+K+  +LF+  +L          
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--------- 306

Query: 306 GKINPVALFFSLFLGKADELVGILKTTFPELGLTKEDCIETTWIESTLIAANGFQNGGAA 365
            +    A F  L+LG   +L+ ++K  FPELGL   +C E +WIES L         G +
Sbjct: 307 KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIK-----GES 366

Query: 366 LEALLSRTPPTNGSTKIKSDYVKEPITKAAIEGIWERLKSQDIEAANLIFVPYGGRMSQI 425
           +E +L++   T+ S K K D+++EPI K AI+ +W R ++ +   A +I  P+GG+MS+I
Sbjct: 367 ME-ILAKRKRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 426

Query: 426 SESEIPFSHRAGNLYKIGYVVGGKEQ---SMDAEKKYLKWIRELYSYMTPFVSKSPRAAY 485
           +++EIPF HR GNLY+I Y+    E+   +    +KYL+W+  +Y +MTP+VSKSPR AY
Sbjct: 427 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNNKYG-KTSYERARVWGLKYFGNNFNRLVHVKTKVDPYNFFRHEQSIPTL 538
           VN+RD+D+G       KT YE A+VWG+KYF NNF+RLV VKT VDP +FF  EQSIP +
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529