Lcy06g000310 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy06g000310
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Pectate lyase
Location	Chr06: 378511 .. 379828 (+)
RNA-Seq Expression	Lcy06g000310
Synteny	Lcy06g000310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATGGGCACCCAACTCTTCGTTCTCTCTCTCATTTCCCTCGCTCTCCTCTCCGCAGTTTCTTATTCCGAAGCCCAAGGCTTGAACATGAACGCCATCGACAAATGCTGGAGATCCAACCCATACTGGAGAAGAAACAGGCAGCAACTCGCCGTTTGTTCGGTGGGCTTCGTCGGAAAAATGACCAACAACATCGGACGAAACCTCATACGCTACGAAGTCACCGACCCCAGCGACGACCCAATAAACCCCCGCCCAGGCACACTCCGACACGGCGCCACAATGATCAGAGCCAAAGTTTGGATCACATTCCGAAAGGACATGCACATTGTGCTTAAGAAGCCGCTTCTCATAAGCAGCTTCACGGCCATCGACGGCCGCGGCGCCACAGTCCACATCACCGGGAATGCCTGTCTCATGGTCTTCAGGGCCACCAATGTCATAATCCATGGCCTCATCATCCACCACTGCAAGCCTCAGTCTGCCGTCCAAGTCATGGGCCCCAATTCCAAGATCGTTTCCTTGGGACACGTGGACGGCGATGCCATTCGATTGGTCACTGCGTCTAAGGTCTGGATCGACCACAACACGCTTTACCGGTGTGACGATGGGCTTATTGACGTCACCAGAGGCTCCACTGACATCACCATTTCCAACAATTGGTTTAGAGACCAAGACAAGGTTATTCTTCTCGGTCACGACGACGGTTATATCCGAGACAGGAACATGAAAGTCACTGTCGTATATAACCACTTCGGACCCAATTGCAACCAGAGAATGCCTAGGTAATGTAAATAACATGGTATAAGAACAAGAAGTCTTGAATTCAAACTCTAACTCTGTAATCTGTATTTGAATTTAGGATTCGATACGGGTATGCGCACGTGACGAACAATTTGTACCAAGGGTGGACGCAATACGCAATCGGAGGGAGCATGAATCCAAGTGTCAAGAGTGAAGCCAACCTTTTCATCGCATCAAAATCAAAACAGGCAACAATAATATATATATAATATTATTCCATGTGTGTTGGTTTAAATTATACAAGCAATTGAACGTAATTATTGAATTGAAATTGACTTGGTGGAGACAGGTTATATGGAGCACAGGGAGAGTAGAGGAGACAAAGTGGAAGTTTCATTCAGTAAGAGACGTTTTTGAAAATGGAGCTTCGTTTGCACAAATTGGCGTGGGGCGAGGAAGTGTGAAGCCAAATTACAACGCCCAACAGCGGTTTCGAGTTGCAGATGCCAAGTGGCTGAGATCATTGACAAGCTCCTCCGGGGCTTTGAGCTGCTCAGCCAACTCAAGATGTTGA

mRNA sequence

AAAATGGGCACCCAACTCTTCGTTCTCTCTCTCATTTCCCTCGCTCTCCTCTCCGCAGTTTCTTATTCCGAAGCCCAAGGCTTGAACATGAACGCCATCGACAAATGCTGGAGATCCAACCCATACTGGAGAAGAAACAGGCAGCAACTCGCCGTTTGTTCGGTGGGCTTCGTCGGAAAAATGACCAACAACATCGGACGAAACCTCATACGCTACGAAGTCACCGACCCCAGCGACGACCCAATAAACCCCCGCCCAGGCACACTCCGACACGGCGCCACAATGATCAGAGCCAAAGTTTGGATCACATTCCGAAAGGACATGCACATTGTGCTTAAGAAGCCGCTTCTCATAAGCAGCTTCACGGCCATCGACGGCCGCGGCGCCACAGTCCACATCACCGGGAATGCCTGTCTCATGGTCTTCAGGGCCACCAATGTCATAATCCATGGCCTCATCATCCACCACTGCAAGCCTCAGTCTGCCGTCCAAGTCATGGGCCCCAATTCCAAGATCGTTTCCTTGGGACACGTGGACGGCGATGCCATTCGATTGGTCACTGCGTCTAAGGTCTGGATCGACCACAACACGCTTTACCGGTGTGACGATGGGCTTATTGACGTCACCAGAGGCTCCACTGACATCACCATTTCCAACAATTGGTTTAGAGACCAAGACAAGGTTATTCTTCTCGGTCACGACGACGGTTATATCCGAGACAGGAACATGAAAGTCACTGTCGTATATAACCACTTCGGACCCAATTGCAACCAGAGAATGCCTAGGATTCGATACGGGTATGCGCACGTGACGAACAATTTGTACCAAGGGTGGACGCAATACGCAATCGGAGGGAGCATGAATCCAAGTGTCAAGAGGAGAGTAGAGGAGACAAAGTGGAAGTTTCATTCAGTAAGAGACGTTTTTGAAAATGGAGCTTCGTTTGCACAAATTGGCGTGGGGCGAGGAAGTGTGAAGCCAAATTACAACGCCCAACAGCGGTTTCGAGTTGCAGATGCCAAGTGGCTGAGATCATTGACAAGCTCCTCCGGGGCTTTGAGCTGCTCAGCCAACTCAAGATGTTGA

Coding sequence (CDS)

ATGGGCACCCAACTCTTCGTTCTCTCTCTCATTTCCCTCGCTCTCCTCTCCGCAGTTTCTTATTCCGAAGCCCAAGGCTTGAACATGAACGCCATCGACAAATGCTGGAGATCCAACCCATACTGGAGAAGAAACAGGCAGCAACTCGCCGTTTGTTCGGTGGGCTTCGTCGGAAAAATGACCAACAACATCGGACGAAACCTCATACGCTACGAAGTCACCGACCCCAGCGACGACCCAATAAACCCCCGCCCAGGCACACTCCGACACGGCGCCACAATGATCAGAGCCAAAGTTTGGATCACATTCCGAAAGGACATGCACATTGTGCTTAAGAAGCCGCTTCTCATAAGCAGCTTCACGGCCATCGACGGCCGCGGCGCCACAGTCCACATCACCGGGAATGCCTGTCTCATGGTCTTCAGGGCCACCAATGTCATAATCCATGGCCTCATCATCCACCACTGCAAGCCTCAGTCTGCCGTCCAAGTCATGGGCCCCAATTCCAAGATCGTTTCCTTGGGACACGTGGACGGCGATGCCATTCGATTGGTCACTGCGTCTAAGGTCTGGATCGACCACAACACGCTTTACCGGTGTGACGATGGGCTTATTGACGTCACCAGAGGCTCCACTGACATCACCATTTCCAACAATTGGTTTAGAGACCAAGACAAGGTTATTCTTCTCGGTCACGACGACGGTTATATCCGAGACAGGAACATGAAAGTCACTGTCGTATATAACCACTTCGGACCCAATTGCAACCAGAGAATGCCTAGGATTCGATACGGGTATGCGCACGTGACGAACAATTTGTACCAAGGGTGGACGCAATACGCAATCGGAGGGAGCATGAATCCAAGTGTCAAGAGGAGAGTAGAGGAGACAAAGTGGAAGTTTCATTCAGTAAGAGACGTTTTTGAAAATGGAGCTTCGTTTGCACAAATTGGCGTGGGGCGAGGAAGTGTGAAGCCAAATTACAACGCCCAACAGCGGTTTCGAGTTGCAGATGCCAAGTGGCTGAGATCATTGACAAGCTCCTCCGGGGCTTTGAGCTGCTCAGCCAACTCAAGATGTTGA

Protein sequence

MGTQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKRRVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC

Homology

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BLAST of Lcy06g000310 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 306.6 bits (784), Expect = 3.7e-82
Identity = 170/384 (44.27%), Postives = 229/384 (59.64%), Query Frame = 0


Query: 5   LFVLSLISLALLSAV---SYSEA---QGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVG 64
           L ++SL+  A  S+V   +YS       L  N ID CWR NPYW  NR+ LA C+VGF G
Sbjct: 6   LTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF-G 65

Query: 65  KMTNNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLIS 124
           K     G+    Y VT+PSDDP NPRPGTLR+ A +    +WITF +DM IVL+  L+++
Sbjct: 66  KSAVG-GKYGSIYVVTNPSDDPENPRPGTLRY-AVIQSKPLWITFARDMVIVLRNELIMN 125

Query: 125 SFTAIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVD 184
           S+  IDGRGA V I    C+ +   ++VIIHG+ IH CKP  + +V    + + S    D
Sbjct: 126 SYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKGSD 185

Query: 185 GDAIRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIR 244
           GDAI +  +S +WIDH    RC DGLIDV   ST +TISNN+F   DKV+LLGH+D  + 
Sbjct: 186 GDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNNVE 245

Query: 245 DRNMKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPS--------- 304
           D+ M+VT+ +NHFGP   +RMPR+R GYAHV NN Y+ W  YAIGGS +P+         
Sbjct: 246 DKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNYFV 305

Query: 305 ---------VKRRVEE----TKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRV 361
                    V +R++      +WK+ + +DVF+NGA F  +  G G+V P Y   +RF V
Sbjct: 306 ASDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGRAERFPV 365

[Close]

BLAST of Lcy06g000310 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.5e-80
Identity = 172/379 (45.38%), Postives = 222/379 (58.58%), Query Frame = 0


Query: 5   LFVLSLISLALLSAVSYS-EAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNN 64
           L + +  S  L +A SY+  A  +++N ID CWR NP W  NRQ LA C+VG+ GK    
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGY-GKAAIG 69

Query: 65  IGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTAI 124
            G+N   Y VT+PSD+P  P PGTLR+  +  +  +WITF +DM IVLK  L+I+S+  I
Sbjct: 70  -GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPK-PLWITFARDMVIVLKSQLMINSYKTI 129

Query: 125 DGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIR 184
           DGRGA V I    CL + +  +VIIHG+ IH CK         PN        +DGD IR
Sbjct: 130 DGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKAD-------PNG-------MDGDGIR 189

Query: 185 LVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMK 244
           +  ++ VWIDH  L RC DGLIDV   ST +TISNN+F   DKV+LLGHDD Y+ D++M+
Sbjct: 190 VFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMR 249

Query: 245 VTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSV------------- 304
           VT+ +N FGP   +RMPR+R GYAHV NN Y+ W  YAIGGS NP +             
Sbjct: 250 VTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEKR 309

Query: 305 ------KRRV---EETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKW 361
                 KR +   +  +WK+ + RDVF NGA F   GV    V+P Y   + FRVA    
Sbjct: 310 SSKQVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGGEGFRVAHGSL 368

[Close]

BLAST of Lcy06g000310 vs. ExPASy Swiss-Prot
Match: O65456 (Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.0e-79
Identity = 159/354 (44.92%), Postives = 210/354 (59.32%), Query Frame = 0


Query: 29  MNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDPINPRPGTL 88
           MN +D CWR    W  NR+ LA C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 89  RHGATMIRAK-VWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNVI 148
           R+G  +I+AK +WITF KDM I L+  L+++S+  IDGRGA V I    C+ +   TNVI
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 149 IHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLIDV 208
           +HG+ IH CKP     V    + +      DGDAI +  +S +WIDH  L  C DGLIDV
Sbjct: 166 VHGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDV 225

Query: 209 TRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGYA 268
              ST ITISNN+F   DKV+LLGH+D +++D  MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 269 HVTNNLYQGWTQYAIGGSMNPSV-------------------KRRVEE--TKWKFHSVRD 328
           HV NN Y  W  YAIGGS +P++                   KR V+     W++ + +D
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKD 345

Query: 329 VFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC 361
           VF+NGA F  +  G GS+   Y++ QRF VA    + SLT+ +G L+C+ N  C
Sbjct: 346 VFKNGAYF--VPSGYGSISLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

[Close]

BLAST of Lcy06g000310 vs. ExPASy Swiss-Prot
Match: O65457 (Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 159/354 (44.92%), Postives = 210/354 (59.32%), Query Frame = 0


Query: 29  MNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDPINPRPGTL 88
           MN +D CWR    W  NR+ LA C+VGF          N+  Y VT+P D+  NP PG+L
Sbjct: 46  MNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTNPYDNAQNPHPGSL 105

Query: 89  RHGATMIRAK-VWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNVI 148
           R+G  +I+AK +WITF KDM I L   L+++S+  IDGRGA V I    C+ +   TNVI
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 149 IHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLIDV 208
           +HG+ IH CKP  + +V    + +      DGDAI +  +S VWIDH  L  C DGLIDV
Sbjct: 166 VHGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDV 225

Query: 209 TRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGYA 268
              ST ITISNN+F   DKV+LLGH+D +++D  MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 269 HVTNNLYQGWTQYAIGGSMNPSV-------------------KRRVEE--TKWKFHSVRD 328
           HV NN Y  W  YAIGGS +P++                   KR V+     W++ +  D
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSND 345

Query: 329 VFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC 361
           VF+NGA F  +  G GS+   Y++ QRF VA    + SLT+ +G L+C+ N  C
Sbjct: 346 VFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

[Close]

BLAST of Lcy06g000310 vs. ExPASy Swiss-Prot
Match: Q9LRM5 (Probable pectate lyase 9 OS=Arabidopsis thaliana OX=3702 GN=At3g24230 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 160/357 (44.82%), Postives = 202/357 (56.58%), Query Frame = 0


Query: 28  NMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDP-INPRPG 87
           N N ID CWR +  W +NR+ LA C +GF  K     GRN   Y VTD SDD  +NP+PG
Sbjct: 102 NGNLIDDCWRCDRNWNKNRKHLADCGMGFGSKAFG--GRNGSYYVVTDHSDDDVVNPKPG 161

Query: 88  TLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNV 147
           TLRH    +   +WI F++DM I LK+ L+++SF  ID RGA VHI   AC+ +   TNV
Sbjct: 162 TLRHAVIQVE-PLWIIFKRDMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNV 221

Query: 148 IIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLID 207
           I+HGL IH CK    V V    S+    G  DGDAI +  +S +WIDHN+L  C DGL+D
Sbjct: 222 IVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVD 281

Query: 208 VTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGY 267
           V  GST ITISNN F   D+V+LLGH+D Y RD+ M+VTV YNHFG    QRMPR R+GY
Sbjct: 282 VVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGY 341

Query: 268 AHVTNNLYQGWTQYAIGGSMNPSVKRR------------VEETK-----------WKFHS 327
            HV NN Y  W  YAIGGS NP++  +             E TK           W + S
Sbjct: 342 FHVVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNHSAKEVTKRLDTKGNEWMEWNWRS 401

Query: 328 VRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC 361
            +D+  NGA F   G G        +   +     A  + ++T+S+GALSC     C
Sbjct: 402 EKDLLVNGAFFTPSGEGASGDSQTLSLPAK----PASMVDAITASAGALSCRRGKPC 451

[Close]

BLAST of Lcy06g000310 vs. ExPASy TrEMBL
Match: A0A6J1E7R9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.7e-184
Identity = 313/376 (83.24%), Postives = 339/376 (90.16%), Query Frame = 0


Query: 3   TQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTN 62
           TQLFVLSLISL LLSAVSYSE  GL MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTN
Sbjct: 4   TQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTN 63

Query: 63  NIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTA 122
           NIGRNLIRY+VTDPSDDPINPRPGTLR+GATMI++KVWITF+KDMHI+L KPLLISS+TA
Sbjct: 64  NIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYTA 123

Query: 123 IDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAI 182
           IDGRGA+VHI+GNACL+V +ATN+IIHGLIIHHCK Q A QVMGPNSK+VSLGHVDGDAI
Sbjct: 124 IDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAI 183

Query: 183 RLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNM 242
           RLV+ASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGH+DGY+RD+NM
Sbjct: 184 RLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKNM 243

Query: 243 KVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR---------- 302
           +VTVVYNHFGPNCNQRMPRIR+GYAHV NNLYQGWTQYAIGGSMNPSVK           
Sbjct: 244 RVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKS 303

Query: 303 --------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRS 361
                   +VE  KWKFHSVRDVFENGASF QIGVGRG VKPNY+AQQRF VADAKW+RS
Sbjct: 304 KQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRS 363

[Close]

BLAST of Lcy06g000310 vs. ExPASy TrEMBL
Match: A0A6J1KLU6 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.9e-182
Identity = 310/376 (82.45%), Postives = 337/376 (89.63%), Query Frame = 0


Query: 3   TQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTN 62
           TQLFVLSLISL LLSAVSYSE  GL MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTN
Sbjct: 4   TQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTN 63

Query: 63  NIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTA 122
           NIGRNLIRY+VTDPSDDPINPRPGTLR+GATMI++KVWITF+K MHI+L KPLLISS+TA
Sbjct: 64  NIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKGMHILLHKPLLISSYTA 123

Query: 123 IDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAI 182
           IDGRGA+VHI+GNACL+V +ATN+IIHGLIIHHCK Q A QVMGPNSK+VSLGHVDGDAI
Sbjct: 124 IDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAI 183

Query: 183 RLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNM 242
           RLV+ASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGH+DGY+RD+NM
Sbjct: 184 RLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKNM 243

Query: 243 KVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR---------- 302
           +VTV+YNHFGPNCNQRMPRIR+GYAHV NNLYQGWTQYAIGGSMNPSVK           
Sbjct: 244 RVTVIYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKL 303

Query: 303 --------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRS 361
                   +VE  KWKFHSV DVFENGASF QIGVGRG VKPNY+AQQRF VADAKW+RS
Sbjct: 304 KQIVWSTGKVEAAKWKFHSVGDVFENGASFEQIGVGRGGVKPNYSAQQRFPVADAKWVRS 363

[Close]

BLAST of Lcy06g000310 vs. ExPASy TrEMBL
Match: A0A0A0LPZ1 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G031910 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.7e-181
Identity = 306/377 (81.17%), Postives = 338/377 (89.66%), Query Frame = 0


Query: 4   QLFVLSLISLALL--SAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMT 63
           QL VLSLIS  LL  +AVSYSEA GLNMNAID+CWRSNPYWR+NRQQLA CSVGF GKMT
Sbjct: 8   QLSVLSLISFILLISTAVSYSEAHGLNMNAIDRCWRSNPYWRKNRQQLATCSVGFAGKMT 67

Query: 64  NNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFT 123
           NN+GRNL+ Y+VTDPSDDPINPRPGTLR+GATMI+ KVWITF+KDMHIVL+KPLL+SSFT
Sbjct: 68  NNMGRNLMHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFKKDMHIVLQKPLLVSSFT 127

Query: 124 AIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDA 183
           AIDGRG+TVHITGNACLMV RATN+IIHGLIIHHCK Q+A QVMGPN+K++SLG+VDGDA
Sbjct: 128 AIDGRGSTVHITGNACLMVVRATNIIIHGLIIHHCKSQAAGQVMGPNAKVISLGNVDGDA 187

Query: 184 IRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRN 243
           IRLVTASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGHDDGY+RDR+
Sbjct: 188 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRS 247

Query: 244 MKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR--------- 303
           M+VTV+YNHFGPNCNQRMPRIRYGYAHV NNLYQGWTQYAIGGSMNPSVK          
Sbjct: 248 MRVTVLYNHFGPNCNQRMPRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 307

Query: 304 ---------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLR 361
                    +VEE KWKFHSVRD FENGASFA+IGVG+G VKPNYN QQRF VADAKW+R
Sbjct: 308 SKQVIWSTGKVEEAKWKFHSVRDAFENGASFAEIGVGKGRVKPNYNPQQRFPVADAKWIR 367

[Close]

BLAST of Lcy06g000310 vs. ExPASy TrEMBL
Match: A0A1S3AY18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 8.0e-181
Identity = 309/377 (81.96%), Postives = 333/377 (88.33%), Query Frame = 0


Query: 4   QLFVLSLISLALL--SAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMT 63
           QL VLSLIS   L  + VS SEA GL+MNAID+CWRSNPYWRRNRQQLA CSVGF GKMT
Sbjct: 6   QLSVLSLISFIFLISNVVSSSEAHGLSMNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMT 65

Query: 64  NNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFT 123
           NN+GRNLI Y+VTDPSDDPINPRPGTLR+GATMI+ KVWITF+KDMHIVL+KPLLISSFT
Sbjct: 66  NNMGRNLIHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFQKDMHIVLQKPLLISSFT 125

Query: 124 AIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDA 183
           AIDGRGATVHITGNACLMV RATN+IIHGLIIHHC+ Q+A +VMGPNSK++SLGHVDGDA
Sbjct: 126 AIDGRGATVHITGNACLMVLRATNIIIHGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDA 185

Query: 184 IRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRN 243
           IRLVTASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGHDDGY+RDRN
Sbjct: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRN 245

Query: 244 MKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR--------- 303
           MKVTVVYNHFGPNCNQRMPRIRYGYAHV NNLYQGWTQYAIGGSMNPSVK          
Sbjct: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 305

Query: 304 ---------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLR 361
                     VEE KWKFHSVRDVFENGA F +IGVGRG VKPNYN QQRF VADAKW+R
Sbjct: 306 SKQVIWSTGNVEEAKWKFHSVRDVFENGALFTEIGVGRGRVKPNYNPQQRFPVADAKWIR 365

[Close]

BLAST of Lcy06g000310 vs. ExPASy TrEMBL
Match: A0A6J1C826 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.0e-175
Identity = 294/350 (84.00%), Postives = 314/350 (89.71%), Query Frame = 0


Query: 29  MNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDPINPRPGTL 88
           MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTNNIGRNLIRY+VTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 89  RHGATMIRAKVWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNVII 148
           R+GA++IR KVWITFR+DM I L KPL+ISSFTAIDGRGATVHI GNACLMV RA+N+II
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 149 HGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLIDVT 208
           HGLIIHHC+ Q+A QVMGPNSKI+SLGHVDGDAIRLVTASKVWIDHNTLYRC+DGL+DVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 209 RGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGYAH 268
           RGSTDITISNNWFRDQDKVILLGHDDGY+RDR MKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 269 VTNNLYQGWTQYAIGGSMNPSVKR------------------RVEETKWKFHSVRDVFEN 328
           V NN+YQGWTQYAIGGSMNPSVK                    VEETKW FHSVRDVFEN
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEVVWSTGNVEETKWNFHSVRDVFEN 300

Query: 329 GASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC 361
           GASFAQIGVGRG V PNYNAQQRF+VADAKW+RSLT+SSGAL C+ NSRC
Sbjct: 301 GASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 350

[Close]

BLAST of Lcy06g000310 vs. NCBI nr
Match: XP_022923821.1 (putative pectate lyase 2 [Cucurbita moschata])

HSP 1 Score: 654.4 bits (1687), Expect = 5.5e-184
Identity = 313/376 (83.24%), Postives = 339/376 (90.16%), Query Frame = 0


Query: 3   TQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTN 62
           TQLFVLSLISL LLSAVSYSE  GL MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTN
Sbjct: 4   TQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTN 63

Query: 63  NIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTA 122
           NIGRNLIRY+VTDPSDDPINPRPGTLR+GATMI++KVWITF+KDMHI+L KPLLISS+TA
Sbjct: 64  NIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYTA 123

Query: 123 IDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAI 182
           IDGRGA+VHI+GNACL+V +ATN+IIHGLIIHHCK Q A QVMGPNSK+VSLGHVDGDAI
Sbjct: 124 IDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAI 183

Query: 183 RLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNM 242
           RLV+ASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGH+DGY+RD+NM
Sbjct: 184 RLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKNM 243

Query: 243 KVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR---------- 302
           +VTVVYNHFGPNCNQRMPRIR+GYAHV NNLYQGWTQYAIGGSMNPSVK           
Sbjct: 244 RVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKS 303

Query: 303 --------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRS 361
                   +VE  KWKFHSVRDVFENGASF QIGVGRG VKPNY+AQQRF VADAKW+RS
Sbjct: 304 KQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRS 363

[Close]

BLAST of Lcy06g000310 vs. NCBI nr
Match: KAG7020030.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 654.1 bits (1686), Expect = 7.2e-184
Identity = 312/376 (82.98%), Postives = 339/376 (90.16%), Query Frame = 0


Query: 3   TQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTN 62
           TQLFVLSLISL LLSAVSYSE  GL MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTN
Sbjct: 4   TQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTN 63

Query: 63  NIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTA 122
           N+GRNLIRY+VTDPSDDPINPRPGTLR+GATMI++KVWITF+KDMHI+L KPLLISS+TA
Sbjct: 64  NVGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYTA 123

Query: 123 IDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAI 182
           IDGRGA+VHI+GNACL+V +ATN+IIHGLIIHHCK Q A QVMGPNSK+VSLGHVDGDAI
Sbjct: 124 IDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAI 183

Query: 183 RLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNM 242
           RLV+ASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGH+DGY+RD+NM
Sbjct: 184 RLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKNM 243

Query: 243 KVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR---------- 302
           +VTVVYNHFGPNCNQRMPRIR+GYAHV NNLYQGWTQYAIGGSMNPSVK           
Sbjct: 244 RVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKS 303

Query: 303 --------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRS 361
                   +VE  KWKFHSVRDVFENGASF QIGVGRG VKPNY+AQQRF VADAKW+RS
Sbjct: 304 KQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRS 363

[Close]

BLAST of Lcy06g000310 vs. NCBI nr
Match: XP_023520094.1 (putative pectate lyase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.7 bits (1680), Expect = 3.6e-183
Identity = 311/376 (82.71%), Postives = 338/376 (89.89%), Query Frame = 0


Query: 3   TQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTN 62
           TQLFVLSLISL LLSAVSYSE  GL MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTN
Sbjct: 4   TQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTN 63

Query: 63  NIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTA 122
           N+GRNLIRY+VTDPSDDPINPRPGTLR+GATM+++KVWITF+KDMHI+L KPLLISS+TA
Sbjct: 64  NVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYTA 123

Query: 123 IDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAI 182
           IDGRGA+VHI+GNACL+V +ATN+IIHGLIIHHCK Q A QVMGPNSK+VSLGHVDGDAI
Sbjct: 124 IDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAI 183

Query: 183 RLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNM 242
           RLV+ASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGH+DGY+RD NM
Sbjct: 184 RLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMNM 243

Query: 243 KVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR---------- 302
           +VTVVYNHFGPNCNQRMPRIR+GYAHV NNLYQGWTQYAIGGSMNPSVK           
Sbjct: 244 RVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKS 303

Query: 303 --------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRS 361
                   +VE  KWKFHSVRDVFENGASF QIGVGRG VKPNY+AQQRF VADAKW+RS
Sbjct: 304 KQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRS 363

[Close]

BLAST of Lcy06g000310 vs. NCBI nr
Match: XP_023001119.1 (putative pectate lyase 2 [Cucurbita maxima])

HSP 1 Score: 648.3 bits (1671), Expect = 4.0e-182
Identity = 310/376 (82.45%), Postives = 337/376 (89.63%), Query Frame = 0


Query: 3   TQLFVLSLISLALLSAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTN 62
           TQLFVLSLISL LLSAVSYSE  GL MNAID+CWRSNPYWRRNRQQL VCSVGFVGKMTN
Sbjct: 4   TQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTN 63

Query: 63  NIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTA 122
           NIGRNLIRY+VTDPSDDPINPRPGTLR+GATMI++KVWITF+K MHI+L KPLLISS+TA
Sbjct: 64  NIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKGMHILLHKPLLISSYTA 123

Query: 123 IDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAI 182
           IDGRGA+VHI+GNACL+V +ATN+IIHGLIIHHCK Q A QVMGPNSK+VSLGHVDGDAI
Sbjct: 124 IDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAI 183

Query: 183 RLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNM 242
           RLV+ASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGH+DGY+RD+NM
Sbjct: 184 RLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKNM 243

Query: 243 KVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR---------- 302
           +VTV+YNHFGPNCNQRMPRIR+GYAHV NNLYQGWTQYAIGGSMNPSVK           
Sbjct: 244 RVTVIYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKL 303

Query: 303 --------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRS 361
                   +VE  KWKFHSV DVFENGASF QIGVGRG VKPNY+AQQRF VADAKW+RS
Sbjct: 304 KQIVWSTGKVEAAKWKFHSVGDVFENGASFEQIGVGRGGVKPNYSAQQRFPVADAKWVRS 363

[Close]

BLAST of Lcy06g000310 vs. NCBI nr
Match: KGN63970.1 (hypothetical protein Csa_014255 [Cucumis sativus])

HSP 1 Score: 643.7 bits (1659), Expect = 9.7e-181
Identity = 306/377 (81.17%), Postives = 338/377 (89.66%), Query Frame = 0


Query: 4   QLFVLSLISLALL--SAVSYSEAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMT 63
           QL VLSLIS  LL  +AVSYSEA GLNMNAID+CWRSNPYWR+NRQQLA CSVGF GKMT
Sbjct: 8   QLSVLSLISFILLISTAVSYSEAHGLNMNAIDRCWRSNPYWRKNRQQLATCSVGFAGKMT 67

Query: 64  NNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFT 123
           NN+GRNL+ Y+VTDPSDDPINPRPGTLR+GATMI+ KVWITF+KDMHIVL+KPLL+SSFT
Sbjct: 68  NNMGRNLMHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFKKDMHIVLQKPLLVSSFT 127

Query: 124 AIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDA 183
           AIDGRG+TVHITGNACLMV RATN+IIHGLIIHHCK Q+A QVMGPN+K++SLG+VDGDA
Sbjct: 128 AIDGRGSTVHITGNACLMVVRATNIIIHGLIIHHCKSQAAGQVMGPNAKVISLGNVDGDA 187

Query: 184 IRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRN 243
           IRLVTASKVWIDHNTLYRC+DGL+DVTRGSTDITISNNWFRDQDKVILLGHDDGY+RDR+
Sbjct: 188 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRS 247

Query: 244 MKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVKR--------- 303
           M+VTV+YNHFGPNCNQRMPRIRYGYAHV NNLYQGWTQYAIGGSMNPSVK          
Sbjct: 248 MRVTVLYNHFGPNCNQRMPRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 307

Query: 304 ---------RVEETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLR 361
                    +VEE KWKFHSVRD FENGASFA+IGVG+G VKPNYN QQRF VADAKW+R
Sbjct: 308 SKQVIWSTGKVEEAKWKFHSVRDAFENGASFAEIGVGKGRVKPNYNPQQRFPVADAKWIR 367

[Close]

BLAST of Lcy06g000310 vs. TAIR 10
Match: AT5G09280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 4.0e-108
Identity = 171/232 (73.71%), Postives = 208/232 (89.66%), Query Frame = 0


Query: 60  MTNNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISS 119
           MT NIG+ + +Y+VTDPSDDP+NP+PGTLR+GAT+++ K WITF+++M I L KPLLISS
Sbjct: 1   MTGNIGKGVTQYKVTDPSDDPLNPKPGTLRYGATLVKGKKWITFKRNMKIKLHKPLLISS 60

Query: 120 FTAIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDG 179
           FTA+DGRGA+VHI+G ACL+V+RAT+VIIHGL IH CK      VMGP+SKI+ LGH+DG
Sbjct: 61  FTALDGRGASVHISGPACLIVYRATDVIIHGLKIHDCKAHPPSSVMGPDSKIIQLGHMDG 120

Query: 180 DAIRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRD 239
           DAIRLVTA KVWIDHNTLY C+DGL+DVT GSTD+T+SNNWFR+QDKV+LLGHDDGY++D
Sbjct: 121 DAIRLVTAKKVWIDHNTLYDCEDGLLDVTLGSTDVTVSNNWFRNQDKVMLLGHDDGYVKD 180

Query: 240 RNMKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSVK 292
           ++M+VTVV+NHFGPNCNQRMPR+R+GYAHV NN YQGWTQYAIGGSM+P VK
Sbjct: 181 KDMRVTVVFNHFGPNCNQRMPRVRHGYAHVANNYYQGWTQYAIGGSMSPRVK 232

[Close]

BLAST of Lcy06g000310 vs. TAIR 10
Match: AT1G11920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 2.6e-83
Identity = 170/384 (44.27%), Postives = 229/384 (59.64%), Query Frame = 0


Query: 5   LFVLSLISLALLSAV---SYSEA---QGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVG 64
           L ++SL+  A  S+V   +YS       L  N ID CWR NPYW  NR+ LA C+VGF G
Sbjct: 6   LTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF-G 65

Query: 65  KMTNNIGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLIS 124
           K     G+    Y VT+PSDDP NPRPGTLR+ A +    +WITF +DM IVL+  L+++
Sbjct: 66  KSAVG-GKYGSIYVVTNPSDDPENPRPGTLRY-AVIQSKPLWITFARDMVIVLRNELIMN 125

Query: 125 SFTAIDGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVD 184
           S+  IDGRGA V I    C+ +   ++VIIHG+ IH CKP  + +V    + + S    D
Sbjct: 126 SYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKGSD 185

Query: 185 GDAIRLVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIR 244
           GDAI +  +S +WIDH    RC DGLIDV   ST +TISNN+F   DKV+LLGH+D  + 
Sbjct: 186 GDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNNVE 245

Query: 245 DRNMKVTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPS--------- 304
           D+ M+VT+ +NHFGP   +RMPR+R GYAHV NN Y+ W  YAIGGS +P+         
Sbjct: 246 DKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNYFV 305

Query: 305 ---------VKRRVEE----TKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRV 361
                    V +R++      +WK+ + +DVF+NGA F  +  G G+V P Y   +RF V
Sbjct: 306 ASDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGRAERFPV 365

[Close]

BLAST of Lcy06g000310 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.5e-81
Identity = 172/379 (45.38%), Postives = 222/379 (58.58%), Query Frame = 0


Query: 5   LFVLSLISLALLSAVSYS-EAQGLNMNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNN 64
           L + +  S  L +A SY+  A  +++N ID CWR NP W  NRQ LA C+VG+ GK    
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGY-GKAAIG 69

Query: 65  IGRNLIRYEVTDPSDDPINPRPGTLRHGATMIRAKVWITFRKDMHIVLKKPLLISSFTAI 124
            G+N   Y VT+PSD+P  P PGTLR+  +  +  +WITF +DM IVLK  L+I+S+  I
Sbjct: 70  -GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPK-PLWITFARDMVIVLKSQLMINSYKTI 129

Query: 125 DGRGATVHITGNACLMVFRATNVIIHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIR 184
           DGRGA V I    CL + +  +VIIHG+ IH CK         PN        +DGD IR
Sbjct: 130 DGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKAD-------PNG-------MDGDGIR 189

Query: 185 LVTASKVWIDHNTLYRCDDGLIDVTRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMK 244
           +  ++ VWIDH  L RC DGLIDV   ST +TISNN+F   DKV+LLGHDD Y+ D++M+
Sbjct: 190 VFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMR 249

Query: 245 VTVVYNHFGPNCNQRMPRIRYGYAHVTNNLYQGWTQYAIGGSMNPSV------------- 304
           VT+ +N FGP   +RMPR+R GYAHV NN Y+ W  YAIGGS NP +             
Sbjct: 250 VTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEKR 309

Query: 305 ------KRRV---EETKWKFHSVRDVFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKW 361
                 KR +   +  +WK+ + RDVF NGA F   GV    V+P Y   + FRVA    
Sbjct: 310 SSKQVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGGEGFRVAHGSL 368

[Close]

BLAST of Lcy06g000310 vs. TAIR 10
Match: AT4G22080.1 (root hair specific 14 )

HSP 1 Score: 298.5 bits (763), Expect = 7.2e-81
Identity = 159/354 (44.92%), Postives = 210/354 (59.32%), Query Frame = 0


Query: 29  MNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDPINPRPGTL 88
           MN +D CWR    W  NR+ LA C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 89  RHGATMIRAK-VWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNVI 148
           R+G  +I+AK +WITF KDM I L+  L+++S+  IDGRGA V I    C+ +   TNVI
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 149 IHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLIDV 208
           +HG+ IH CKP     V    + +      DGDAI +  +S +WIDH  L  C DGLIDV
Sbjct: 166 VHGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDV 225

Query: 209 TRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGYA 268
              ST ITISNN+F   DKV+LLGH+D +++D  MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 269 HVTNNLYQGWTQYAIGGSMNPSV-------------------KRRVEE--TKWKFHSVRD 328
           HV NN Y  W  YAIGGS +P++                   KR V+     W++ + +D
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKD 345

Query: 329 VFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC 361
           VF+NGA F  +  G GS+   Y++ QRF VA    + SLT+ +G L+C+ N  C
Sbjct: 346 VFKNGAYF--VPSGYGSISLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

[Close]

BLAST of Lcy06g000310 vs. TAIR 10
Match: AT4G22090.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 9.4e-81
Identity = 159/354 (44.92%), Postives = 210/354 (59.32%), Query Frame = 0


Query: 29  MNAIDKCWRSNPYWRRNRQQLAVCSVGFVGKMTNNIGRNLIRYEVTDPSDDPINPRPGTL 88
           MN +D CWR    W  NR+ LA C+VGF          N+  Y VT+P D+  NP PG+L
Sbjct: 46  MNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTNPYDNAQNPHPGSL 105

Query: 89  RHGATMIRAK-VWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFRATNVI 148
           R+G  +I+AK +WITF KDM I L   L+++S+  IDGRGA V I    C+ +   TNVI
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 149 IHGLIIHHCKPQSAVQVMGPNSKIVSLGHVDGDAIRLVTASKVWIDHNTLYRCDDGLIDV 208
           +HG+ IH CKP  + +V    + +      DGDAI +  +S VWIDH  L  C DGLIDV
Sbjct: 166 VHGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDV 225

Query: 209 TRGSTDITISNNWFRDQDKVILLGHDDGYIRDRNMKVTVVYNHFGPNCNQRMPRIRYGYA 268
              ST ITISNN+F   DKV+LLGH+D +++D  MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 269 HVTNNLYQGWTQYAIGGSMNPSV-------------------KRRVEE--TKWKFHSVRD 328
           HV NN Y  W  YAIGGS +P++                   KR V+     W++ +  D
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSND 345

Query: 329 VFENGASFAQIGVGRGSVKPNYNAQQRFRVADAKWLRSLTSSSGALSCSANSRC 361
           VF+NGA F  +  G GS+   Y++ QRF VA    + SLT+ +G L+C+ N  C
Sbjct: 346 VFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

The following BLAST results are available for this feature:

BLAST of Lcy06g000310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65388	3.7e-82	44.27	Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2	[more]
Q9C8G4	3.5e-80	45.38	Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1	[more]
O65456	1.0e-79	44.92	Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1	[more]
O65457	1.3e-79	44.92	Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1	[more]
Q9LRM5	1.1e-78	44.82	Probable pectate lyase 9 OS=Arabidopsis thaliana OX=3702 GN=At3g24230 PE=2 SV=1	[more]

BLAST of Lcy06g000310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E7R9	2.7e-184	83.24	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1	[more]
A0A6J1KLU6	1.9e-182	82.45	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1	[more]
A0A0A0LPZ1	4.7e-181	81.17	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G031910 PE=3 SV=1	[more]
A0A1S3AY18	8.0e-181	81.96	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1	[more]
A0A6J1C826	3.0e-175	84.00	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1	[more]

BLAST of Lcy06g000310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923821.1	5.5e-184	83.24	putative pectate lyase 2 [Cucurbita moschata]	[more]
KAG7020030.1	7.2e-184	82.98	putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023520094.1	3.6e-183	82.71	putative pectate lyase 2 [Cucurbita pepo subsp. pepo]	[more]
XP_023001119.1	4.0e-182	82.45	putative pectate lyase 2 [Cucurbita maxima]	[more]
KGN63970.1	9.7e-181	81.17	hypothetical protein Csa_014255 [Cucumis sativus]	[more]

BLAST of Lcy06g000310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09280.1	4.0e-108	73.71	Pectin lyase-like superfamily protein	[more]
AT1G11920.1	2.6e-83	44.27	Pectin lyase-like superfamily protein	[more]
AT1G30350.1	2.5e-81	45.38	Pectin lyase-like superfamily protein	[more]
AT4G22080.1	7.2e-81	44.92	root hair specific 14	[more]
AT4G22090.1	9.4e-81	44.92	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 114..130 score: 50.7 coord: 258..277 score: 61.43 coord: 77..102 score: 43.04 coord: 178..199 score: 48.48 coord: 280..299 score: 35.95
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 106..296 e-value: 1.0E-58 score: 211.1
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 119..285 e-value: 4.8E-22 score: 78.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 29..353 e-value: 2.1E-86 score: 292.0
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 295..357
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 11..292
None	No IPR available	PANTHER	PTHR31683:SF74	PECTATE LYASE	coord: 11..292
None	No IPR available	PANTHER	PTHR31683:SF74	PECTATE LYASE	coord: 295..357
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 29..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy06g000310.1	Lcy06g000310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity