Lcy05g015540 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g015540
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	O-methyltransferase family protein
Location	Chr05: 19678761 .. 19678997 (-)
RNA-Seq Expression	Lcy05g015540
Synteny	Lcy05g015540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATTGGTAGTAAAGATTCCAATTTCAAGAAAGAAGAAGAAGATAGTGAAGTAAGAGTTCGAGTTTGGAAATACATATTTGGTTTCGTCGAAATGGCAGTAATAAAATGTGCCATAGAGCTTAAAATCGCAGATGCCATCGAAAGTCATGGAAGTCCCATAACACTGCCACAACTATCCCCTGCTACAAATTGCTCCGATCCCTCGCTTTTGCACCGCGTCATGCGCTTCTTG

mRNA sequence

ATGGATATTGGTAGTAAAGATTCCAATTTCAAGAAAGAAGAAGAAGATAGTGAAGTAAGAGTTCGAGTTTGGAAATACATATTTGGTTTCGTCGAAATGGCAGTAATAAAATGTGCCATAGAGCTTAAAATCGCAGATGCCATCGAAAGTCATGGAAGTCCCATAACACTGCCACAACTATCCCCTGCTACAAATTGCTCCGATCCCTCGCTTTTGCACCGCGTCATGCGCTTCTTG

Coding sequence (CDS)

ATGGATATTGGTAGTAAAGATTCCAATTTCAAGAAAGAAGAAGAAGATAGTGAAGTAAGAGTTCGAGTTTGGAAATACATATTTGGTTTCGTCGAAATGGCAGTAATAAAATGTGCCATAGAGCTTAAAATCGCAGATGCCATCGAAAGTCATGGAAGTCCCATAACACTGCCACAACTATCCCCTGCTACAAATTGCTCCGATCCCTCGCTTTTGCACCGCGTCATGCGCTTCTTG

Protein sequence

MDIGSKDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSPATNCSDPSLLHRVMRFL

Homology

[Close]

BLAST of Lcy05g015540 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.0e-12
Identity = 37/76 (48.68%), Postives = 55/76 (72.37%), Query Frame = 0


Query: 6  KDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGS--PITLPQLSPA 65
          +D+  K  +E+++  + +WKY+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LS A
Sbjct: 16 EDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSA 75

Query: 66 TNCSDPSLLHRVMRFL 80
           + S PS L R+MRFL
Sbjct: 76 VSAS-PSHLRRIMRFL 90

[Close]

BLAST of Lcy05g015540 vs. ExPASy Swiss-Prot
Match: Q84KK4 (Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.0e-10
Identity = 35/81 (43.21%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 1  MDIGSKDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 60
          MD  S  SN  ++ E S+ ++ ++K+++ FV    +K A+EL IAD I SHG PITLP+L
Sbjct: 1  MDFSS--SNGSEDTELSQAQIHLYKHVYNFVSSMALKSAMELGIADVIHSHGKPITLPEL 60

Query: 61 SPATNC--SDPSLLHRVMRFL 80
          + A N   S   +LHR +R L
Sbjct: 61 ATALNLRPSKIGVLHRFLRLL 79

[Close]

BLAST of Lcy05g015540 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 3.0e-08
Identity = 29/65 (44.62%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 22 RVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSPATNCSDPS-------LLHR 80
          ++W+ +F FV+   +KCAIEL+IAD I SHG PITL Q++     +  S        L R
Sbjct: 9  QIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNIPYLSR 68

[Close]

BLAST of Lcy05g015540 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.1e-07
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 8  SNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSPATNCS 67
          +    ++E  E +  VW +I+ ++    +KCAI+L I DAI  HG+PITL QL+ A N +
Sbjct: 5  NGISSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNIN 64

Query: 68 DPSL--LHRVMRFL 80
                L R+MR L
Sbjct: 65 KAKSHGLFRLMRIL 78

[Close]

BLAST of Lcy05g015540 vs. ExPASy Swiss-Prot
Match: O24305 ((+)-6a-hydroxymaackiain 3-O-methyltransferase 1 OS=Pisum sativum OX=3888 GN=HMM1 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.9e-07
Identity = 28/74 (37.84%), Postives = 47/74 (63.51%), Query Frame = 0


Query: 8  SNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSPATNC- 67
          +N  +E E    ++ ++K+++ FV    +K A+EL IADAI +HG P+TLP+LS +    
Sbjct: 5  TNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLPELSSSLKLH 64

Query: 68 -SDPSLLHRVMRFL 80
           S  ++L+R +R L
Sbjct: 65 PSKVNILYRFLRLL 78

[Close]

BLAST of Lcy05g015540 vs. ExPASy TrEMBL
Match: A0A6J1HWX9 ((RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468232 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 8.3e-22
Identity = 53/78 (67.95%), Postives = 68/78 (87.18%), Query Frame = 0


Query: 2  DIGSKDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLS 61
          +IGSKDS FK+EEE ++++++VWKYIFGFVEMAVIKCAIELKIAD I++HG  +TLP+LS
Sbjct: 3  NIGSKDSKFKREEEGNDIKIQVWKYIFGFVEMAVIKCAIELKIADTIDAHGGLMTLPELS 62

Query: 62 PATNCSDPSLLHRVMRFL 80
           + NCS P LL R++RFL
Sbjct: 63 SSLNCS-PLLLRRILRFL 79

[Close]

BLAST of Lcy05g015540 vs. ExPASy TrEMBL
Match: A0A6J1C1G3 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007584 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.1e-21
Identity = 58/70 (82.86%), Postives = 61/70 (87.14%), Query Frame = 0


Query: 11 KKEEED-SEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSPATNCSDP 70
          KKE+ED SE R +VWKYIFGFVEMAVIKCAIELKI DAIESHGSPITLPQLS A  CS P
Sbjct: 7  KKEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPP 66

Query: 71 SLLHRVMRFL 80
          S LHRV+RFL
Sbjct: 67 S-LHRVLRFL 75

[Close]

BLAST of Lcy05g015540 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.5e-20
Identity = 54/79 (68.35%), Postives = 66/79 (83.54%), Query Frame = 0


Query: 2  DIGSKDSN-FKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
          D G+K+SN  K EEE+S+++V+VWKYIFGFVEMAV+KCAIELKI D IESHG  ITL QL
Sbjct: 3  DTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLSQL 62

Query: 62 SPATNCSDPSLLHRVMRFL 80
          S + +CS P LLHR++RFL
Sbjct: 63 SSSLSCS-PLLLHRILRFL 80

[Close]

BLAST of Lcy05g015540 vs. ExPASy TrEMBL
Match: A0A6J1I1Q8 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468230 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.5e-20
Identity = 55/80 (68.75%), Postives = 69/80 (86.25%), Query Frame = 0


Query: 1  MDIGSKDSNFKK-EEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQ 60
          M+I SK+S++KK EEE+ E +++++KYIFGF E AVIKCAIEL+IADAIESHGSP+TL Q
Sbjct: 1  MEITSKESSWKKEEEEEEEAKIQIYKYIFGFAEAAVIKCAIELQIADAIESHGSPMTLSQ 60

Query: 61 LSPATNCSDPSLLHRVMRFL 80
          LS A NCS PSLL R++RFL
Sbjct: 61 LSSALNCS-PSLLFRILRFL 79

[Close]

BLAST of Lcy05g015540 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.5e-20
Identity = 55/79 (69.62%), Postives = 64/79 (81.01%), Query Frame = 0


Query: 2  DIGSKDSN-FKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
          D  SK SN  K EEEDS+++V++WKYIFGFVEMAVIKCAIELKI D IESHG  +TL QL
Sbjct: 3  DTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQL 62

Query: 62 SPATNCSDPSLLHRVMRFL 80
          S + NCS P LLHR++RFL
Sbjct: 63 SSSLNCS-PLLLHRILRFL 80

[Close]

BLAST of Lcy05g015540 vs. NCBI nr
Match: XP_022969151.1 ((RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 112.5 bits (280), Expect = 1.7e-21
Identity = 53/78 (67.95%), Postives = 68/78 (87.18%), Query Frame = 0


Query: 2  DIGSKDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLS 61
          +IGSKDS FK+EEE ++++++VWKYIFGFVEMAVIKCAIELKIAD I++HG  +TLP+LS
Sbjct: 3  NIGSKDSKFKREEEGNDIKIQVWKYIFGFVEMAVIKCAIELKIADTIDAHGGLMTLPELS 62

Query: 62 PATNCSDPSLLHRVMRFL 80
           + NCS P LL R++RFL
Sbjct: 63 SSLNCS-PLLLRRILRFL 79

[Close]

BLAST of Lcy05g015540 vs. NCBI nr
Match: XP_022135686.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 110.2 bits (274), Expect = 8.5e-21
Identity = 58/70 (82.86%), Postives = 61/70 (87.14%), Query Frame = 0


Query: 11 KKEEED-SEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSPATNCSDP 70
          KKE+ED SE R +VWKYIFGFVEMAVIKCAIELKI DAIESHGSPITLPQLS A  CS P
Sbjct: 7  KKEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPP 66

Query: 71 SLLHRVMRFL 80
          S LHRV+RFL
Sbjct: 67 S-LHRVLRFL 75

[Close]

BLAST of Lcy05g015540 vs. NCBI nr
Match: XP_023554129.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108.6 bits (270), Expect = 2.5e-20
Identity = 52/79 (65.82%), Postives = 67/79 (84.81%), Query Frame = 0


Query: 1  MDIGSKDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 60
          M+I SK+S++KKEEE+ E +++++KY+FGF E AVIKCA+ELKIADAIESHG P+TL QL
Sbjct: 1  MEITSKESSWKKEEEEEEAKIQIYKYVFGFAEAAVIKCAVELKIADAIESHGRPMTLSQL 60

Query: 61 SPATNCSDPSLLHRVMRFL 80
          S A NCS P LL R++RFL
Sbjct: 61 SSALNCS-PPLLFRILRFL 78

[Close]

BLAST of Lcy05g015540 vs. NCBI nr
Match: KAG6572141.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107.8 bits (268), Expect = 4.2e-20
Identity = 54/79 (68.35%), Postives = 67/79 (84.81%), Query Frame = 0


Query: 2  DIGSKDSNFK-KEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
          DIGSKDS  K +EEE+++++V+VWKYIFGFVEMAVIKCAIELKI D IE+HG  +TL +L
Sbjct: 3  DIGSKDSKLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTEL 62

Query: 62 SPATNCSDPSLLHRVMRFL 80
          S + NCS P LLHR++RFL
Sbjct: 63 SSSLNCS-PLLLHRILRFL 80

[Close]

BLAST of Lcy05g015540 vs. NCBI nr
Match: XP_022969149.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 107.1 bits (266), Expect = 7.2e-20
Identity = 55/80 (68.75%), Postives = 69/80 (86.25%), Query Frame = 0


Query: 1  MDIGSKDSNFKK-EEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQ 60
          M+I SK+S++KK EEE+ E +++++KYIFGF E AVIKCAIEL+IADAIESHGSP+TL Q
Sbjct: 1  MEITSKESSWKKEEEEEEEAKIQIYKYIFGFAEAAVIKCAIELQIADAIESHGSPMTLSQ 60

Query: 61 LSPATNCSDPSLLHRVMRFL 80
          LS A NCS PSLL R++RFL
Sbjct: 61 LSSALNCS-PSLLFRILRFL 79

[Close]

BLAST of Lcy05g015540 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 72.8 bits (177), Expect = 1.4e-13
Identity = 37/76 (48.68%), Postives = 55/76 (72.37%), Query Frame = 0


Query: 6  KDSNFKKEEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGS--PITLPQLSPA 65
          +D+  K  +E+++  + +WKY+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LS A
Sbjct: 16 EDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSA 75

Query: 66 TNCSDPSLLHRVMRFL 80
           + S PS L R+MRFL
Sbjct: 76 VSAS-PSHLRRIMRFL 90

[Close]

BLAST of Lcy05g015540 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 7.0e-13
Identity = 34/68 (50.00%), Postives = 51/68 (75.00%), Query Frame = 0


Query: 14 EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGS--PITLPQLSPATNCSDPSL 73
          +E+++  + +W+Y+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LS A + S PS 
Sbjct: 10 DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSAS-PSH 69

Query: 74 LHRVMRFL 80
          L R+MRFL
Sbjct: 70 LRRIMRFL 76

The following BLAST results are available for this feature:

BLAST of Lcy05g015540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	2.0e-12	48.68	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
Q84KK4	7.0e-10	43.21	Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV...	[more]
B0ZB55	3.0e-08	44.62	Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P...	[more]
Q6VMW0	1.1e-07	39.19	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
O24305	1.9e-07	37.84	(+)-6a-hydroxymaackiain 3-O-methyltransferase 1 OS=Pisum sativum OX=3888 GN=HMM1...	[more]

BLAST of Lcy05g015540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HWX9	8.3e-22	67.95	(RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=...	[more]
A0A6J1C1G3	4.1e-21	82.86	(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A5D3DI94	3.5e-20	68.35	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1I1Q8	3.5e-20	68.75	xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468...	[more]
A0A0A0K0Q2	3.5e-20	69.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1	[more]

BLAST of Lcy05g015540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022969151.1	1.7e-21	67.95	(RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 [Cucurbita maxima]	[more]
XP_022135686.1	8.5e-21	82.86	(R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia]	[more]
XP_023554129.1	2.5e-20	65.82	xanthohumol 4'-O-methyltransferase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6572141.1	4.2e-20	68.35	Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022969149.1	7.2e-20	68.75	xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima]	[more]

BLAST of Lcy05g015540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	1.4e-13	48.68	O-methyltransferase family protein	[more]
AT4G35150.1	7.0e-13	50.00	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 35..79 e-value: 6.8E-10 score: 38.8
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 5..79 e-value: 2.1E-19 score: 71.0
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 9..79
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 24..79 score: 15.447467
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 9..79
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 13..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g015540.1	Lcy05g015540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity