Lcy05g014590 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g014590
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionU6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase
LocationChr05: 17944559 .. 17949404 (-)
RNA-Seq ExpressionLcy05g014590
SyntenyLcy05g014590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAATGGAAAAATGGGAGCGCCGGCGTTCGTTCGTTCAGTGCAGAGAGGTCGAAGAAGAAGAAGGAAAAAATGGTAGCGCCAGCGTTCGTTGTTCAGTGCAGAGAGGTCGCGAAGAAGAAGATGGAAAAATGGGTAGCTGGGGTTTCTTTCACGCACGGGGGAAAAATGGGAGGGAAAGAAGAAAGTGTTCCAATTTTTTGTTTTTTCCTTCATTAATTACACGCGCGCGGGCCCTCTTTTTGTTTATTTGTTTTTTTTCATAGCTTATTTTAAAAAATGCTATCCTAAAAAGCTATAAAAAGGGTTATTTCTTGTAGTGAAGTATTCTTATATGCTCTTTTTTTTTTTGAAAAATATTCTTATATGCTTTGAAAGCATATGCACGATACAAAATTAATAAAAAATTCAAAGAAAAAATGACTTTAGAGATAATCTTTTGTTAGAATTTTTTAGTTTTGGCAATATTATTTTATGGTTAATCTGTGAACTAATCAAACTTCAATCATTCATATATATATATATGGATTCGAACTCTAACCTCTTAGTCGAAAATATATTCCAATTACCACTTAGTTATGCTCAGTTTGACTCAAACATCAAAATTTAACCGCCAACAAGAACTTAGCTCAATTGGCATAACTTTGTACTGATAACCACAAGGTCTGTGGTTCGAATCTCCCTTCCCTCATATTGTCAAAAAAAAAAAAAAAAAATCAAAATTTAACCCATGTCCTAATAAATATGTCCCTGAGATTTTCGTTTTTTGAATATTTTTTGACAACACATGGAAGTAGAAAGATTCGAACCACTAAACTCTTAGGGCCTGTTTGTTTTATGTTTTCGGATTCACTGCGTTTGGCTGTGTTTTTTAAAACTGTTTTCGGATTCTGTGAACCAAATAGTAGAAATTCTTAAAACAATTTTTTATATGTTTTCAGTGTATCCAGATTTTGAATTTGAAATTTTCAAATGCAAAATTAAATTAAAAAAGATAAAAACTTTAATCAATATAGTATTTCAGGTCATAAACTCAATTCATTTTTATAAAATTAAAATTTGTATAATATAATCTATTATAAATTATATGATATTTTAAAATAATAATTATACAAAATTTATAATACGTAACATGTTTATAAATTAATTAATAATAATTTATATTCAACTTAATATATTTAAATGATGACTATAAATAGTTTATAATTTTTAAATATATTATCAAAAGTATTTTGAACCATTATTTAAATTAAGATTTAATTTCTCAATCTACTACCAAACATATATTTGAAAACATGAAATACAATACTGTTTTGATTGAATCATTTGTTTTCAAAATTTTGTTTTCAGATCACCAACCTAACACACACTAATCTTGTTTTGTTAATAATTTGGTGCAGCATAGCAGTCCCCCTGCTACAGTGTGCAGGAGCGAACACCCAAGAAGAAGAAGAAGAAGAAGAGAGCCGTTTGAAAAAGAGGTGACGGTGCGAAATTTGGCGATGACGAGAAAGAAGAGGAAGACGAGAGAGCCACGAACAATCCACCCCAGAAACAAATACGCCGAAAATCCCCCAGATTTCGCTCTCTTAGCCTCTCTATACCCTTCTTTTCAACCCTTCGTCTTTCTCGATCCCCGACCCAGAATCGACTGGACCGACTTCAATGCCACCCGAGAGCTTACCCGGGTTTTGCTTCTCCACGACCATGTTCTTCATTGGTACTATTCTACTTTTTTCTTTCCCCATAGCGGAAGCTTAGAAATTTCTGTTGCTGTTCTGCTTTCGTTTACTTCTCTTTTCTGCTGCTAGTATGTCAGCTAAGTTAAATTTGGACTTCCATTATTGCAAAACTCTGATGGAAACTAGGGGGGGTTGGCTTTCTGTGGATGCATTCAATTTTTTTAGGATTTCATAGGATTATGGCTAGGCTAGTTTTATCCCTTTTGAAGTGATTAGGAGATATGCTAACCCAGGTCATTATTTTTAAGAGGATCAACACTGTATACCGTATGTGGGTTATTGTTATTTTTCAATTTTGAACTTGTTTGAAATTGTGTCTCTTGATTCGAGGAATCATGTGTTTTGAGAGTTCTTTCATTGTGTTTCGCTTGTTGGTTTGTTGGCGATGTGAAAAGGCTTTGTTTGTCAAATTAGGATGGCTTTGTGATTGAAGTGTAAATAGATCTCTGCATCTCAGGTGGATTCCTGATGGGCAGCTCTGCCCAACAGTACCTAATAGATTAAATTATATCCATTGGCTTGAAGATCTTTTGTCATCTGATATCATTGCAAAGGCAAATAGTGATAGAGGTCGTGTAAGAGGTTTTGATATAGGAACTGGAGCAAATTGCATTTATCCACTTCTTGGAGCCTCTCTTCTCTGGTGGAGTTTCGTTGGGACAGGTGACTTCCTTACCATATAGTTGGTTTTGTTCTCCAAATTCTATATTACCCATTGATTTGTATGTTTAACAATGACCAGGTTTTGGGTTCCGCTCTGCTTGGTGCAGATGTGACTGATGTAGCACTAGAGTGGGCTGAAAGAAATGTTAAAAGTAATCCACACGTTTCTGAACTTATTGAAATTAGAAAGGTAGATAGTACCTCGGACGTTCTTACTACAGAATTACATAATGGTGTAGCAGTTGAAAGTGAAAACAAGAATTTAAATGATATAGATGGTAAGGAGGTACGGCTGCCGTTGCCCTCTTCCTCATTAAAGGAGACAGTCTACTTGGAGAAGAGATATCATGGTCCTCCTATACTTCTTGGTGTTGTCAAAGATGGCGAGGAGTTTGACTTTTGCATGTGCAATCCTCCCTTTTTTGAGAGTATGGATGAAGCAGGGTTGAATCGGAAGACTTCTTGTGGCGGAACTCCTCAAGAGATGGTTTGTCCAGGTGGGGAGAGGGCGTTTATTAGTCGTATGATTGAAGACAGCACTGTCTTGAAGCAGACTTTCCGGTATTCCTTGCTCTTACAACTGCAAAGTTGCCTCTGATTCTTCTCTATACCTTTTAGGAATCTTATCCTTTTATCTTCATTGATCTTTGCAGGTGGTATACTTCAATGATTGGGAAGAAATCAAACCTCAGCTTCCTGACATCTAAACTTTGGAAAGTTGGAGTGACAGTCGTCAAGACTACAGAATTTGTCCAAGGCCAAACTTGCCGATGGGGACTTGCTTGGTCTTTTATGCCTGCAGCACGGAAGATTATATCTCCTCATGTTACTAACAAGAGTGTCTTGTCTTTCATGCTCGAGGTGGATAATTGATAGAGCTTCTTAATTTCTTTTGTATTGGGCTTACACAGACCTCAGATTCGTCAATCAAATCTTTTCTATGTTTGTTGAGATATTAATCCCTTTCTGTAGATATGTAGCTCAGTTTTGATATCTTTCTGTTGCTCAGGGCCTCCTTCCACAAGTCAGTGCCATGAGTATACTGCAATCAGTTGAACTTTTTCTGAGCACCGGTGGAGCTTCTTGTAAACTGAACACTTCTTCATTTACAGTAGATGTATGGTTGTTCACTTGGCTTCTTTTATATCTTTGCAAGTGATGATATCATGCTAACAAGTTACATTACAACAAATGTTTTTGTAGATTACTGCATCTGAGGATCATTGCCAAGCAATTCTCAAGAATGGGGCATTCGAGGTGGACAAAGGTGCTGGTTGTGTAACTGTGGAAAAATCAGAGCCTCAACTCTCGAGTGATCTGTGTTTTCGAGTTTCGGTATTTTCCTGCTTGTCCATTTATGGTCGTGGAACTTCATTACTAATGCTTTGCCCATACTTTTGACTATCGCAATTTTCTTTGTTATAGGTATTTCAGCAAATCCCTGGCACGCTTCTTGTGAAAGGCTCCTTGCATCATAAGAGTAGTCCTTCTGCAGGTACTACTCGCATATGCATTTGAACTTTCTTTTGATTGTAATTTATGTGGACTTCCACAAAAACTATAGAAATGAATTTTTGTTTTTTAAATATATTTTAAAAGAAACTATCGATTAGTATTACCAACAAATTGAACTAAATGAAAGCTCAGTTTCTCAAAGAAGAAAAAAATAAAACAGATTACAAACAAGAATACAAGAGATTTCCAGGGAGTGAAAGAGCTTTTAATCTATTTTCAACACTCTCTTTCACGCATTTGACTCAAATATTTGACAAGGATCAACACTGGAACTGTTATTAGGAAATAGAGAGAAAATAACCTCCTACTCTTGTATGGCTAAGCTTTTGGCTCTTTTAATTTTTTCAATATTATATTCTCAAGAGTTACAAGAAGAAACCCAACTGCCAAGAATTAAAATGCGATTTTGTATATATCTAATATGCATGATGCAACGAAATAGCAATATGACCACGGTGTATATAATTCCTTGCAGGCTTATTCTCACTGATTTTCCACCGATTAGAGGAATTCCTGAAAGCTGAATCTTGTAAAGAAAAGGTGTTATAGAGATCCCTCTGCCTCAACCAACAATACTTCTTATGTAGAAGCTCCACAACAGGGCAACTGTATTGCTTGCTATGCAGGGGCAGAGCGAAGAAAGGAGGGACCAATGTTGTAATTCAAAATCTATATAGTTTTGTAGTTTTCCTCTAATTTTCTCTTTTAATTTTTTGAAGTTAAAAGATTATTATTTTTGGATCTTTTGAAGCTTGAATTCTAGTCTTCTGCAAGAACAGTCACAGTTTACAGTAGGTAAGCCTAGAAAACTACCATTGTATTATTACTTGTTTGTTTCCAATACATAGCCCATGAACTATGTGCTGGACTCGCAATTGTACACTTTATGTCATAGAGCAATGAAGGATCTTGATCATATTTTG

mRNA sequence

ATGGAAAAATGGAAAAATGGGAGCGCCGGCGTTCGTTCGTTCAGTGCAGAGAGGTCGAAGAAGAAGAAGGAAAAAATGGTAGCGCCAGCGTTCGTTGTTCAGTGCAGAGAGGTCGCGAAGAAGAAGATGGAAAAATGGGTAGCTGGGCATAGCAGTCCCCCTGCTACAGTGTGCAGGAGCGAACACCCAAGAAGAAGAAGAAGAAGAAGAGAGCCGTTTGAAAAAGAGGTGACGGTGCGAAATTTGGCGATGACGAGAAAGAAGAGGAAGACGAGAGAGCCACGAACAATCCACCCCAGAAACAAATACGCCGAAAATCCCCCAGATTTCGCTCTCTTAGCCTCTCTATACCCTTCTTTTCAACCCTTCGTCTTTCTCGATCCCCGACCCAGAATCGACTGGACCGACTTCAATGCCACCCGAGAGCTTACCCGGGTTTTGCTTCTCCACGACCATGTTCTTCATTGGTGGATTCCTGATGGGCAGCTCTGCCCAACAGTACCTAATAGATTAAATTATATCCATTGGCTTGAAGATCTTTTGTCATCTGATATCATTGCAAAGGCAAATAGTGATAGAGGTCGTGTAAGAGGTTTTGATATAGGAACTGGAGCAAATTGCATTTATCCACTTCTTGGAGCCTCTCTTCTCTGGTGGAGTTTCGTTGGGACAGATGTGACTGATGTAGCACTAGAGTGGGCTGAAAGAAATGTTAAAAGTAATCCACACGTTTCTGAACTTATTGAAATTAGAAAGGTAGATAGTACCTCGGACGTTCTTACTACAGAATTACATAATGGTGTAGCAGTTGAAAGTGAAAACAAGAATTTAAATGATATAGATGGTAAGGAGGTACGGCTGCCGTTGCCCTCTTCCTCATTAAAGGAGACAGTCTACTTGGAGAAGAGATATCATGGTCCTCCTATACTTCTTGGTGTTGTCAAAGATGGCGAGGAGTTTGACTTTTGCATGTGCAATCCTCCCTTTTTTGAGAGTATGGATGAAGCAGGGTTGAATCGGAAGACTTCTTGTGGCGGAACTCCTCAAGAGATGGTTTGTCCAGGTGGGGAGAGGGCGTTTATTAGTCGTATGATTGAAGACAGCACTGTCTTGAAGCAGACTTTCCGGTGGTATACTTCAATGATTGGGAAGAAATCAAACCTCAGCTTCCTGACATCTAAACTTTGGAAAGTTGGAGTGACAGTCGTCAAGACTACAGAATTTGTCCAAGGCCAAACTTGCCGATGGGGACTTGCTTGGTCTTTTATGCCTGCAGCACGGAAGATTATATCTCCTCATGTTACTAACAAGAGTGTCTTGTCTTTCATGCTCGAGGGCCTCCTTCCACAAGTCAGTGCCATGAGTATACTGCAATCAGTTGAACTTTTTCTGAGCACCGGTGGAGCTTCTTGTAAACTGAACACTTCTTCATTTACAGTAGATATTACTGCATCTGAGGATCATTGCCAAGCAATTCTCAAGAATGGGGCATTCGAGGTGGACAAAGGTGCTGGTTGTGTAACTGTGGAAAAATCAGAGCCTCAACTCTCGAGTGATCTGTGTTTTCGAGTTTCGGTATTTCAGCAAATCCCTGGCACGCTTCTTGTGAAAGGCTCCTTGCATCATAAGAGTAGTCCTTCTGCAGGCTTATTCTCACTGATTTTCCACCGATTAGAGGAATTCCTGAAAGCTGAATCTTGTAAAGAAAAGGTGTTATAGAGATCCCTCTGCCTCAACCAACAATACTTCTTATGTAGAAGCTCCACAACAGGGCAACTGTATTGCTTGCTATGCAGGGGCAGAGCGAAGAAAGGAGGGACCAATGTTGTAATTCAAAATCTATATAGTTTTGTAGTTTTCCTCTAATTTTCTCTTTTAATTTTTTGAAGTTAAAAGATTATTATTTTTGGATCTTTTGAAGCTTGAATTCTAGTCTTCTGCAAGAACAGTCACAGTTTACAGTAGGTAAGCCTAGAAAACTACCATTGTATTATTACTTGTTTGTTTCCAATACATAGCCCATGAACTATGTGCTGGACTCGCAATTGTACACTTTATGTCATAGAGCAATGAAGGATCTTGATCATATTTTG

Coding sequence (CDS)

ATGGAAAAATGGAAAAATGGGAGCGCCGGCGTTCGTTCGTTCAGTGCAGAGAGGTCGAAGAAGAAGAAGGAAAAAATGGTAGCGCCAGCGTTCGTTGTTCAGTGCAGAGAGGTCGCGAAGAAGAAGATGGAAAAATGGGTAGCTGGGCATAGCAGTCCCCCTGCTACAGTGTGCAGGAGCGAACACCCAAGAAGAAGAAGAAGAAGAAGAGAGCCGTTTGAAAAAGAGGTGACGGTGCGAAATTTGGCGATGACGAGAAAGAAGAGGAAGACGAGAGAGCCACGAACAATCCACCCCAGAAACAAATACGCCGAAAATCCCCCAGATTTCGCTCTCTTAGCCTCTCTATACCCTTCTTTTCAACCCTTCGTCTTTCTCGATCCCCGACCCAGAATCGACTGGACCGACTTCAATGCCACCCGAGAGCTTACCCGGGTTTTGCTTCTCCACGACCATGTTCTTCATTGGTGGATTCCTGATGGGCAGCTCTGCCCAACAGTACCTAATAGATTAAATTATATCCATTGGCTTGAAGATCTTTTGTCATCTGATATCATTGCAAAGGCAAATAGTGATAGAGGTCGTGTAAGAGGTTTTGATATAGGAACTGGAGCAAATTGCATTTATCCACTTCTTGGAGCCTCTCTTCTCTGGTGGAGTTTCGTTGGGACAGATGTGACTGATGTAGCACTAGAGTGGGCTGAAAGAAATGTTAAAAGTAATCCACACGTTTCTGAACTTATTGAAATTAGAAAGGTAGATAGTACCTCGGACGTTCTTACTACAGAATTACATAATGGTGTAGCAGTTGAAAGTGAAAACAAGAATTTAAATGATATAGATGGTAAGGAGGTACGGCTGCCGTTGCCCTCTTCCTCATTAAAGGAGACAGTCTACTTGGAGAAGAGATATCATGGTCCTCCTATACTTCTTGGTGTTGTCAAAGATGGCGAGGAGTTTGACTTTTGCATGTGCAATCCTCCCTTTTTTGAGAGTATGGATGAAGCAGGGTTGAATCGGAAGACTTCTTGTGGCGGAACTCCTCAAGAGATGGTTTGTCCAGGTGGGGAGAGGGCGTTTATTAGTCGTATGATTGAAGACAGCACTGTCTTGAAGCAGACTTTCCGGTGGTATACTTCAATGATTGGGAAGAAATCAAACCTCAGCTTCCTGACATCTAAACTTTGGAAAGTTGGAGTGACAGTCGTCAAGACTACAGAATTTGTCCAAGGCCAAACTTGCCGATGGGGACTTGCTTGGTCTTTTATGCCTGCAGCACGGAAGATTATATCTCCTCATGTTACTAACAAGAGTGTCTTGTCTTTCATGCTCGAGGGCCTCCTTCCACAAGTCAGTGCCATGAGTATACTGCAATCAGTTGAACTTTTTCTGAGCACCGGTGGAGCTTCTTGTAAACTGAACACTTCTTCATTTACAGTAGATATTACTGCATCTGAGGATCATTGCCAAGCAATTCTCAAGAATGGGGCATTCGAGGTGGACAAAGGTGCTGGTTGTGTAACTGTGGAAAAATCAGAGCCTCAACTCTCGAGTGATCTGTGTTTTCGAGTTTCGGTATTTCAGCAAATCCCTGGCACGCTTCTTGTGAAAGGCTCCTTGCATCATAAGAGTAGTCCTTCTGCAGGCTTATTCTCACTGATTTTCCACCGATTAGAGGAATTCCTGAAAGCTGAATCTTGTAAAGAAAAGGTGTTATAG

Protein sequence

MEKWKNGSAGVRSFSAERSKKKKEKMVAPAFVVQCREVAKKKMEKWVAGHSSPPATVCRSEHPRRRRRRREPFEKEVTVRNLAMTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATRELTRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTGANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFMLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKGAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFLKAESCKEKVL
Homology
BLAST of Lcy05g014590 vs. ExPASy Swiss-Prot
Match: Q554C9 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0275203 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.9e-58
Identity = 138/356 (38.76%), Postives = 200/356 (56.18%), Query Frame = 0

Query: 82  LAMTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVF--LDPRPRIDWTDFNA 141
           L  T+KKRK R   ++H  N Y  NPP+F LLAS YP+F  ++    +    IDW D NA
Sbjct: 28  LKWTKKKRK-RSNDSMHINNFYRYNPPNFKLLASKYPTFDKYIINKTEKIYNIDWKDSNA 87

Query: 142 TRELTRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSS--DIIAKANSDRGRVR 201
           T+ELTRVLL HD  L   +PD  LCPT+  R+NY++W+ D L +   I+   ++D   ++
Sbjct: 88  TKELTRVLLDHDFGLRIELPDNYLCPTLTLRINYLYWISDQLKNLKIILNDNDNDNKIIK 147

Query: 202 GFDIGTGANCIYPLLGASLL-WWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDST 261
           G DIGTG +CI+PLLGA L   WSF+G D+ D  LE+A+ N+  N  ++  I + K +  
Sbjct: 148 GIDIGTGTSCIFPLLGAKLFNNWSFIGIDIDDKVLEYAQNNITIN-SLNSKITLFKNEKN 207

Query: 262 SDVLTTELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKD 321
           SD+L   L+      + N + +D                      +  H          D
Sbjct: 208 SDILLKLLNYKEGSNTFNSSNDD---------------------HQDNHDDD------DD 267

Query: 322 GEEF--DFCMCNPPFFESMDEAGLNR----KTSCGGTPQEMVCPGGERAFISRMIEDSTV 381
            EE+  DFC+CNPPFF+ ++E   N+    K++C G+  EMV  GGE  F+ R+I++S  
Sbjct: 268 DEEYFADFCLCNPPFFKDLNENNNNKNNNPKSNCTGSVNEMVTDGGEFEFVKRIIKESFQ 327

Query: 382 LKQTFRWYTSMIGKKSNLSFLTS---KLWKVGVTVVKTTEFVQGQTCRWGLAWSFM 424
           LK   R+YT+MIG+K NL+ L +   K + +    ++TTE VQG T RW L+W F+
Sbjct: 328 LKCKIRFYTTMIGRKVNLNPLINILIKQYYLPKNQIQTTELVQGNTSRWVLSWYFL 354

BLAST of Lcy05g014590 vs. ExPASy Swiss-Prot
Match: Q6DC64 (RNA N6-adenosine-methyltransferase mettl16 OS=Danio rerio OX=7955 GN=mettl16 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 7.2e-56
Identity = 135/339 (39.82%), Postives = 179/339 (52.80%), Query Frame = 0

Query: 95  RTIHPRNKYAENPPDFALLASLYPSFQPFV--FLDPRPRIDWTDFNATRELTRVLLLHDH 154
           +++HPRN+Y + PPDF  LAS YP FQ  V   L  R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHPRNRYKDKPPDFVYLASKYPEFQKHVQTTLTGRVTLNFKDPEAVRALTCTLLKEDF 64

Query: 155 VLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTGANCIYPLL 214
            L   IP  +L PTVP RLNYIHW+EDL+      + N      RG DIGTGA+CIYPLL
Sbjct: 65  GLTIEIPLERLIPTVPLRLNYIHWVEDLIG----GQGNPQ----RGIDIGTGASCIYPLL 124

Query: 215 GASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTELHNGVAVES 274
           GA++  W F+ T+V D+   +A++NV+ N H++ELI++ KV   +               
Sbjct: 125 GATMNGWFFLATEVDDICFNYAKKNVEQN-HLAELIKVVKVPQKT--------------- 184

Query: 275 ENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCMCNPPFFES 334
                           L   +LKE                   +   +DFCMCNPPFF +
Sbjct: 185 ----------------LLMDALKE-------------------ESIVYDFCMCNPPFFAN 244

Query: 335 MDEA-GLNRKTSCGGTPQ--------EMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 394
             EA G+N + S    P         E++  GGE  F+ R+I DS  LK+  RWY+ M+G
Sbjct: 245 QLEAKGVNSRNSRRPPPSSINTGGVTEIMAEGGELEFVKRVIHDSLQLKKRLRWYSCMLG 284

Query: 395 KKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSF 423
           KK +L+ L  +L K GV  V  TEF QG+T RW LAWSF
Sbjct: 305 KKCSLAPLKDELRKQGVAKVTHTEFCQGRTMRWALAWSF 284

BLAST of Lcy05g014590 vs. ExPASy Swiss-Prot
Match: Q6GR37 (RNA N6-adenosine-methyltransferase mettl16 OS=Xenopus laevis OX=8355 GN=mettl16 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.0e-54
Identity = 137/360 (38.06%), Postives = 190/360 (52.78%), Query Frame = 0

Query: 95  RTIHPRNKYAENPPDFALLASLYPSFQPF--VFLDPRPRIDWTDFNATRELTRVLLLHDH 154
           +++HPRN+Y + PPDFA LAS YP F+    V L  R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHPRNRYKDKPPDFAYLASKYPEFKQHVNVNLAGRVSLNFKDPCAVRALTCTLLKEDF 64

Query: 155 VLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRV-RGFDIGTGANCIYPL 214
            L   IP  +L PTVP RLNYIHW+EDL++       +SD+  + RG DIGTGA+CIYPL
Sbjct: 65  GLTIDIPLERLIPTVPLRLNYIHWVEDLINYH-----DSDKTALRRGIDIGTGASCIYPL 124

Query: 215 LGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTELHNGVAVE 274
           LGA+L  W F+ T+V D+   +A++NV+ N ++S+LI++ KV   +              
Sbjct: 125 LGATLNGWYFLATEVDDICYNYAKKNVEQN-NLSDLIKVVKVPQKT-------------- 184

Query: 275 SENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE--FDFCMCNPPF 334
                                                +L+  +K+  E  +DFCMCNPPF
Sbjct: 185 -------------------------------------LLMDALKEESEIIYDFCMCNPPF 244

Query: 335 FESMDEA-GLNRKTSCGGTPQ--------EMVCPGGERAFISRMIEDSTVLKQTFRWYTS 394
           F +  EA G+N +      P         E++  GGE  F+ R+I DS  LK+  RWY+ 
Sbjct: 245 FANQLEAQGVNSRNPHRSPPSSVNTGGITEIMAEGGELEFVKRIIHDSLKLKKRLRWYSC 304

Query: 395 MIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVL 441
           M+GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF     ++I P+   K  L
Sbjct: 305 MLGKKCSLAPLKEELRLQGVPKVAHTEFYQGRTMRWALAWSFY---EEVIIPNPPKKRKL 304

BLAST of Lcy05g014590 vs. ExPASy Swiss-Prot
Match: Q9CQG2 (RNA N6-adenosine-methyltransferase METTL16 OS=Mus musculus OX=10090 GN=Mettl16 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-54
Identity = 141/381 (37.01%), Postives = 200/381 (52.49%), Query Frame = 0

Query: 95  RTIHPRNKYAENPPDFALLASLYPSFQPF--VFLDPRPRIDWTDFNATRELTRVLLLHDH 154
           +++H RN+Y + PPDFA LAS YP F+    + L+ R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHARNRYKDKPPDFAYLASKYPDFKQHIQINLNGRVSLNFKDPEAVRALTCTLLREDF 64

Query: 155 VLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRV-RGFDIGTGANCIYPL 214
            L   IP  +L PTVP RLNYIHW+EDL     I   +SD+  + RG DIGTGA+CIYPL
Sbjct: 65  GLSIDIPLERLIPTVPLRLNYIHWVEDL-----IGHQDSDKTTLRRGIDIGTGASCIYPL 124

Query: 215 LGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTELHNGVAVE 274
           LGA+L  W F+ T+V D+   +A++NV+ N ++S+LI++ KV   +              
Sbjct: 125 LGATLNGWYFLATEVDDMCFNYAKKNVEQN-NLSDLIKVVKVPQKT-------------- 184

Query: 275 SENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE--FDFCMCNPPF 334
                                                +L+  +K+  E  +DFCMCNPPF
Sbjct: 185 -------------------------------------LLMDALKEESEIVYDFCMCNPPF 244

Query: 335 FESMDEA-GLNRKTSCGGTPQ--------EMVCPGGERAFISRMIEDSTVLKQTFRWYTS 394
           F +  EA G+N + S    P         E++  GGE  F+ R+I DS  LK+  RWY+ 
Sbjct: 245 FANQLEAKGVNSRNSRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSC 304

Query: 395 MIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSF-----MPA----ARKIIS 453
           M+GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF     +P+     RK+  
Sbjct: 305 MLGKKCSLAPLKEELRIQGVPKVTFTEFCQGRTMRWALAWSFYDDVTVPSPPSKRRKLEK 325

BLAST of Lcy05g014590 vs. ExPASy Swiss-Prot
Match: Q86W50 (RNA N6-adenosine-methyltransferase METTL16 OS=Homo sapiens OX=9606 GN=METTL16 PE=1 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 3.0e-54
Identity = 133/342 (38.89%), Postives = 182/342 (53.22%), Query Frame = 0

Query: 95  RTIHPRNKYAENPPDFALLASLYPSFQPFV--FLDPRPRIDWTDFNATRELTRVLLLHDH 154
           +++H RN+Y + PPDFA LAS YP F+  V   L+ R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHARNRYKDKPPDFAYLASKYPDFKQHVQINLNGRVSLNFKDPEAVRALTCTLLREDF 64

Query: 155 VLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRV-RGFDIGTGANCIYPL 214
            L   IP  +L PTVP RLNYIHW+EDL     I   +SD+  + RG DIGTGA+CIYPL
Sbjct: 65  GLSIDIPLERLIPTVPLRLNYIHWVEDL-----IGHQDSDKSTLRRGIDIGTGASCIYPL 124

Query: 215 LGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTELHNGVAVE 274
           LGA+L  W F+ T+V D+   +A++NV+ N ++S+LI++ KV   +              
Sbjct: 125 LGATLNGWYFLATEVDDMCFNYAKKNVEQN-NLSDLIKVVKVPQKT-------------- 184

Query: 275 SENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE--FDFCMCNPPF 334
                                                +L+  +K+  E  +DFCMCNPPF
Sbjct: 185 -------------------------------------LLMDALKEESEIIYDFCMCNPPF 244

Query: 335 FESMDEA-GLNRKTSCGGTPQ--------EMVCPGGERAFISRMIEDSTVLKQTFRWYTS 394
           F +  EA G+N +      P         E++  GGE  F+ R+I DS  LK+  RWY+ 
Sbjct: 245 FANQLEAKGVNSRNPRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSC 289

Query: 395 MIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSF 423
           M+GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF
Sbjct: 305 MLGKKCSLAPLKEELRIQGVPKVTYTEFCQGRTMRWALAWSF 289

BLAST of Lcy05g014590 vs. ExPASy TrEMBL
Match: A0A6J1I146 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468473 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.3e-254
Identity = 436/490 (88.98%), Postives = 454/490 (92.65%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSD++AKAN DRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANRDRGSNVRGFDIG 120

Query: 204 TGANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTT 263
           TGANCIYPLLGASLL WSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD T D LTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 264 ELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 323
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNERKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 324 CMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 383
           CMCNPPFFESMDEAGLN KT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 384 GKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 443
           GKKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 444 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 503
           MLEGLLPQVSAMSILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSAMSILQSVERFLSAGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 504 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFL 563
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHHKSSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVPVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 564 KAESCKEKVL 573
           KAESCKEKVL
Sbjct: 481 KAESCKEKVL 490

BLAST of Lcy05g014590 vs. ExPASy TrEMBL
Match: A0A6J1C2I0 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111007785 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.9e-254
Identity = 435/488 (89.14%), Postives = 457/488 (93.65%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTR KRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 203
           TRVLLLHDH L+WWIPDGQLCPTVPNR NYIHWLEDLLSSDIIA  NSDRG VRGFDIGT
Sbjct: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120

Query: 204 GANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTE 263
           GANCIYPLLGASLL WSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVDSTS+ L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180

Query: 264 LHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 323
           LH+GVAV SE+KN +D+DG+ V  P PSSSL ETVYLEKRYHGPPILL VVKDGE+FDFC
Sbjct: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240

Query: 324 MCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 383
           MCNPPFF SMDEAGLN KTSCGGT QEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300

Query: 384 KKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 443
           KKSNLSFL SKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPAARKIIS HVTNKSVLSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360

Query: 444 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 503
           LEGLLP+V+AMSILQS+E FLSTGGASCKLNTSSFTVDITASEDHCQAILKNGA++VDKG
Sbjct: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420

Query: 504 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFLK 563
           AGCVTV++SE Q S++LCFRVSVFQQIPGTLLVKGSLHH SS SAG+FSLIFHRLEEFLK
Sbjct: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480

Query: 564 AESCKEKV 572
           AESC+EKV
Sbjct: 481 AESCREKV 488

BLAST of Lcy05g014590 vs. ExPASy TrEMBL
Match: A0A6J1GKM3 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455141 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.6e-253
Identity = 434/490 (88.57%), Postives = 453/490 (92.45%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 204 TGANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTT 263
           TGANCIYPLLGASLL WSFVGTDVT+VALEWAERNVKSNPHVSELIEIRKVD T D LT 
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTNVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTI 180

Query: 264 ELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 323
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 324 CMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 383
           CMCNPPFFESMDEAGLN KT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 384 GKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 443
           GKKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 444 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 503
           MLEGLLPQVSA SILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 504 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFL 563
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHHKSSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 564 KAESCKEKVL 573
           KAESCKEKVL
Sbjct: 481 KAESCKEKVL 490

BLAST of Lcy05g014590 vs. ExPASy TrEMBL
Match: A0A1S3BIM5 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103490299 PE=3 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 2.4e-251
Identity = 430/485 (88.66%), Postives = 449/485 (92.58%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLL+SDI+AK NSD GRVRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLASDIVAKKNSDCGRVRGFDIGT 120

Query: 204 GANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTE 263
           GANCIYPLLGASLL WSFVGTDVTDVALEWAE+NVKSNPHVSELIEIRKVD TSD L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAEKNVKSNPHVSELIEIRKVDDTSDNLSTE 180

Query: 264 LHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 323
           LH+ VAV+SE K  N++ G+EV  PLPS  LKE V+LEK+YHGPPILLGVVKDGEEFDFC
Sbjct: 181 LHDSVAVDSECKIFNEMGGREVGPPLPSFLLKEAVHLEKQYHGPPILLGVVKDGEEFDFC 240

Query: 324 MCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 383
           MCNPPFFESMDEAGLN KTSCGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTSQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 384 KKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 443
           KKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFM  ARKIISPHVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 444 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 503
           LEGLLPQVSAMSILQSVE FLSTGGASCKLNTSSFTVDITAS DHC+AI++NGA + DK 
Sbjct: 361 LEGLLPQVSAMSILQSVERFLSTGGASCKLNTSSFTVDITASVDHCRAIIENGACQEDKV 420

Query: 504 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFLK 563
           A CV +EKSE QLS+DLCFRVSVFQQIPGTLLVKGSLH KSSPSAGLFSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSSPSAGLFSLIFHRLEEFLK 480

Query: 564 AESCK 569
           A+ CK
Sbjct: 481 ADFCK 485

BLAST of Lcy05g014590 vs. ExPASy TrEMBL
Match: A0A0A0K5X1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G009800 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.9e-249
Identity = 426/485 (87.84%), Postives = 444/485 (91.55%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFV L PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVSLHPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSD++AK NSD GRVRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKKNSDCGRVRGFDIGT 120

Query: 204 GANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTE 263
           GANCIYPLLGASLL WSFVGTDVTDVALEWAERNV SNPH+SELIEIRKVD TSD L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVTSNPHISELIEIRKVDDTSDDLSTE 180

Query: 264 LHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 323
           LH+ VAV+SE K  N++DG+E+  PLPS   KE ++LE +YHGPPILLGVVKDGE FDFC
Sbjct: 181 LHDSVAVDSECKIFNEMDGREIGPPLPSFLSKEAIHLETQYHGPPILLGVVKDGERFDFC 240

Query: 324 MCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 383
           MCNPPFFESMDEAGLN KTSCGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 384 KKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 443
           KKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFM  ARKIISPHVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 444 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 503
           LEGLL QVSAMSILQSVE FLSTGGASCKLN SSFTVDITASEDHCQAIL+NGA E  K 
Sbjct: 361 LEGLLSQVSAMSILQSVERFLSTGGASCKLNASSFTVDITASEDHCQAILENGACEEVKD 420

Query: 504 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFLK 563
           A CV +EKSE QLS+DLCFRVSVFQQIPGTLLVKGSLH KS+PSAGLFSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSNPSAGLFSLIFHRLEEFLK 480

Query: 564 AESCK 569
           AE CK
Sbjct: 481 AEFCK 485

BLAST of Lcy05g014590 vs. NCBI nr
Match: XP_038886852.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 891.7 bits (2303), Expect = 3.3e-255
Identity = 441/489 (90.18%), Postives = 450/489 (92.02%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSDI+AK NSD G VRG DIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIVAKKNSDSGPVRGLDIGT 120

Query: 204 GANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTE 263
           GANCIYPLLGASLL WSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD  SD L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDISDDLSTE 180

Query: 264 LHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 323
           LHN VAV+SE K LN+ DG EV  PLPSS  KETV+LEKRYHGPPILLGVVKDGEEFDFC
Sbjct: 181 LHNSVAVDSEFKILNEKDGMEVAPPLPSSLSKETVHLEKRYHGPPILLGVVKDGEEFDFC 240

Query: 324 MCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 383
           MCNPPFFESMDEAGLN KTSCGGTPQEMVCPGGERAFISRMIEDS VL+QTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSIVLRQTFRWYTSMIG 300

Query: 384 KKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 443
           KKSNLSFL SKLWKVGVTVVKTTEFVQGQ CRWGLAWSFMP ARKIISPHVTNKSV+SFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQICRWGLAWSFMPTARKIISPHVTNKSVMSFM 360

Query: 444 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 503
           LEGLLPQVSA SILQSVE FLS  GASCKLNTSSFTVDITASEDHC+AILKN A EVDKG
Sbjct: 361 LEGLLPQVSATSILQSVERFLSASGASCKLNTSSFTVDITASEDHCRAILKNRACEVDKG 420

Query: 504 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFLK 563
           A   TVEKSEPQ SSDLCFRVSVFQQIPG+LLVKGSLHHKSSPSAG FSLIFH LEEFLK
Sbjct: 421 ASSATVEKSEPQPSSDLCFRVSVFQQIPGSLLVKGSLHHKSSPSAGSFSLIFHGLEEFLK 480

Query: 564 AESCKEKVL 573
           AESCKEKVL
Sbjct: 481 AESCKEKVL 489

BLAST of Lcy05g014590 vs. NCBI nr
Match: XP_022969493.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita maxima])

HSP 1 Score: 888.6 bits (2295), Expect = 2.8e-254
Identity = 436/490 (88.98%), Postives = 454/490 (92.65%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSD++AKAN DRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANRDRGSNVRGFDIG 120

Query: 204 TGANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTT 263
           TGANCIYPLLGASLL WSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD T D LTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 264 ELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 323
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNERKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 324 CMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 383
           CMCNPPFFESMDEAGLN KT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 384 GKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 443
           GKKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 444 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 503
           MLEGLLPQVSAMSILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSAMSILQSVERFLSAGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 504 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFL 563
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHHKSSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVPVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 564 KAESCKEKVL 573
           KAESCKEKVL
Sbjct: 481 KAESCKEKVL 490

BLAST of Lcy05g014590 vs. NCBI nr
Match: XP_022135953.1 (methyltransferase-like protein 16 homolog isoform X1 [Momordica charantia] >XP_022135955.1 methyltransferase-like protein 16 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 887.1 bits (2291), Expect = 8.0e-254
Identity = 435/488 (89.14%), Postives = 457/488 (93.65%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTR KRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 203
           TRVLLLHDH L+WWIPDGQLCPTVPNR NYIHWLEDLLSSDIIA  NSDRG VRGFDIGT
Sbjct: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120

Query: 204 GANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTTE 263
           GANCIYPLLGASLL WSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVDSTS+ L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180

Query: 264 LHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 323
           LH+GVAV SE+KN +D+DG+ V  P PSSSL ETVYLEKRYHGPPILL VVKDGE+FDFC
Sbjct: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240

Query: 324 MCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 383
           MCNPPFF SMDEAGLN KTSCGGT QEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300

Query: 384 KKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 443
           KKSNLSFL SKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPAARKIIS HVTNKSVLSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360

Query: 444 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 503
           LEGLLP+V+AMSILQS+E FLSTGGASCKLNTSSFTVDITASEDHCQAILKNGA++VDKG
Sbjct: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420

Query: 504 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFLK 563
           AGCVTV++SE Q S++LCFRVSVFQQIPGTLLVKGSLHH SS SAG+FSLIFHRLEEFLK
Sbjct: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480

Query: 564 AESCKEKV 572
           AESC+EKV
Sbjct: 481 AESCREKV 488

BLAST of Lcy05g014590 vs. NCBI nr
Match: XP_022952458.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita moschata])

HSP 1 Score: 883.2 bits (2281), Expect = 1.2e-252
Identity = 434/490 (88.57%), Postives = 453/490 (92.45%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 204 TGANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTT 263
           TGANCIYPLLGASLL WSFVGTDVT+VALEWAERNVKSNPHVSELIEIRKVD T D LT 
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTNVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTI 180

Query: 264 ELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 323
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 324 CMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 383
           CMCNPPFFESMDEAGLN KT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 384 GKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 443
           GKKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 444 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 503
           MLEGLLPQVSA SILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 504 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFL 563
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHHKSSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 564 KAESCKEKVL 573
           KAESCKEKVL
Sbjct: 481 KAESCKEKVL 490

BLAST of Lcy05g014590 vs. NCBI nr
Match: XP_023554274.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.3 bits (2276), Expect = 4.4e-252
Identity = 433/490 (88.37%), Postives = 452/490 (92.24%), Query Frame = 0

Query: 84  MTRKKRKTREPRTIHPRNKYAENPPDFALLASLYPSFQPFVFLDPRPRIDWTDFNATREL 143
           MTRKKRKTRE  TIHPRNKYAENPPDFALLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 144 TRVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 203
           TRVLLLHDH LHWWIPDGQLCPTVPNR NYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 204 TGANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTSDVLTT 263
           TGANCIYPLLGASLL WSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD T D LTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 264 ELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 323
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 324 CMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 383
           CMCNPPFFESMDEAGLN KT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 384 GKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 443
           GKKSNLSFL SKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVSSF 360

Query: 444 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 503
           MLEGLLPQVSA SILQSVE FLS GGASCKLN SSFTVD+TAS DHC+ IL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSASSILQSVERFLSAGGASCKLNISSFTVDVTASNDHCREILENGAYEVDK 420

Query: 504 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSLIFHRLEEFL 563
           GA  V VE SEPQ S+DLCFRVSVFQQIPGTLLVKGSLHHKS+PS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEMSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSTPSSGSFSQIFHRLEEFL 480

Query: 564 KAESCKEKVL 573
           KAESCKEKVL
Sbjct: 481 KAESCKEKVL 490

BLAST of Lcy05g014590 vs. TAIR 10
Match: AT2G21070.1 (methyltransferases )

HSP 1 Score: 589.3 bits (1518), Expect = 3.2e-168
Identity = 303/495 (61.21%), Postives = 362/495 (73.13%), Query Frame = 0

Query: 87  KKRKTREP-RTIHPRNKYAENPPDFALLASLYPSFQPFVFLD-PRPRIDWTDFNATRELT 146
           KKR   EP  T  PR+KY++NPPDFA LASLYPSF+PFVF    R RIDWTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 147 RVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTG 206
           RVLLLHDH ++WWIPDGQLCPTVPNR NYIHW+ DLLSS II     D  +V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 207 ANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIR------KVDSTSD 266
           ANCIYPLLGASL  WSFVG+D T VALEWAE+NV+SNPH S+LIEIR      +  S  +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIRDSKVPPQCSSVPE 184

Query: 267 VLTTELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGE 326
           V  TE    +  E+E                 S+++K   +  K +  P +LLGVVK+ E
Sbjct: 185 VENTEREKTIQEEAE----------------ISATVKSDYHDNKSFIEPAVLLGVVKENE 244

Query: 327 EFDFCMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWY 386
            FDFCM NPPFFE+ +EAGLN KTSCGGTP+EMVC GGE+AF+SR+I+DS VL+Q FRWY
Sbjct: 245 TFDFCMSNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWY 304

Query: 387 TSMIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKS 446
           TSM+GKK+NL  L SKLW+VGVT+VKTTEFVQGQT RWGLAWSFMP ARKII+P V  KS
Sbjct: 305 TSMLGKKANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKS 364

Query: 447 VLSFMLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAF 506
           VLSFMLE +  Q SA+ +LQSVE F  + GASCKLN+S+F+VDI AS D C  I KN   
Sbjct: 365 VLSFMLECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIA 424

Query: 507 EVDK----GAGCVTVEKSEPQLSS-DLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSL 566
           +VD     G    +++ S  Q+   +L FR+ VFQQ+PGTLL+KGSL  K SP +GLFS 
Sbjct: 425 DVDSVRSHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSR 483

Query: 567 IFHRLEEFLKAESCK 569
           +F  LEE LK++ C+
Sbjct: 485 VFGSLEESLKSKFCR 483

BLAST of Lcy05g014590 vs. TAIR 10
Match: AT2G21070.3 (methyltransferases )

HSP 1 Score: 589.3 bits (1518), Expect = 3.2e-168
Identity = 303/495 (61.21%), Postives = 362/495 (73.13%), Query Frame = 0

Query: 87  KKRKTREP-RTIHPRNKYAENPPDFALLASLYPSFQPFVFLD-PRPRIDWTDFNATRELT 146
           KKR   EP  T  PR+KY++NPPDFA LASLYPSF+PFVF    R RIDWTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 147 RVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTG 206
           RVLLLHDH ++WWIPDGQLCPTVPNR NYIHW+ DLLSS II     D  +V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 207 ANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIR------KVDSTSD 266
           ANCIYPLLGASL  WSFVG+D T VALEWAE+NV+SNPH S+LIEIR      +  S  +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIRDSKVPPQCSSVPE 184

Query: 267 VLTTELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGE 326
           V  TE    +  E+E                 S+++K   +  K +  P +LLGVVK+ E
Sbjct: 185 VENTEREKTIQEEAE----------------ISATVKSDYHDNKSFIEPAVLLGVVKENE 244

Query: 327 EFDFCMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWY 386
            FDFCM NPPFFE+ +EAGLN KTSCGGTP+EMVC GGE+AF+SR+I+DS VL+Q FRWY
Sbjct: 245 TFDFCMSNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWY 304

Query: 387 TSMIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKS 446
           TSM+GKK+NL  L SKLW+VGVT+VKTTEFVQGQT RWGLAWSFMP ARKII+P V  KS
Sbjct: 305 TSMLGKKANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKS 364

Query: 447 VLSFMLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAF 506
           VLSFMLE +  Q SA+ +LQSVE F  + GASCKLN+S+F+VDI AS D C  I KN   
Sbjct: 365 VLSFMLECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIA 424

Query: 507 EVDK----GAGCVTVEKSEPQLSS-DLCFRVSVFQQIPGTLLVKGSLHHKSSPSAGLFSL 566
           +VD     G    +++ S  Q+   +L FR+ VFQQ+PGTLL+KGSL  K SP +GLFS 
Sbjct: 425 DVDSVRSHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSR 483

Query: 567 IFHRLEEFLKAESCK 569
           +F  LEE LK++ C+
Sbjct: 485 VFGSLEESLKSKFCR 483

BLAST of Lcy05g014590 vs. TAIR 10
Match: AT2G21070.2 (methyltransferases )

HSP 1 Score: 496.9 bits (1278), Expect = 2.2e-140
Identity = 249/388 (64.18%), Postives = 291/388 (75.00%), Query Frame = 0

Query: 87  KKRKTREP-RTIHPRNKYAENPPDFALLASLYPSFQPFVFLD-PRPRIDWTDFNATRELT 146
           KKR   EP  T  PR+KY++NPPDFA LASLYPSF+PFVF    R RIDWTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 147 RVLLLHDHVLHWWIPDGQLCPTVPNRLNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTG 206
           RVLLLHDH ++WWIPDGQLCPTVPNR NYIHW+ DLLSS II     D  +V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 207 ANCIYPLLGASLLWWSFVGTDVTDVALEWAERNVKSNPHVSELIEIR------KVDSTSD 266
           ANCIYPLLGASL  WSFVG+D T VALEWAE+NV+SNPH S+LIEIR      +  S  +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIRDSKVPPQCSSVPE 184

Query: 267 VLTTELHNGVAVESENKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGE 326
           V  TE    +  E+E                 S+++K   +  K +  P +LLGVVK+ E
Sbjct: 185 VENTEREKTIQEEAE----------------ISATVKSDYHDNKSFIEPAVLLGVVKENE 244

Query: 327 EFDFCMCNPPFFESMDEAGLNRKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWY 386
            FDFCM NPPFFE+ +EAGLN KTSCGGTP+EMVC GGE+AF+SR+I+DS VL+Q FRWY
Sbjct: 245 TFDFCMSNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWY 304

Query: 387 TSMIGKKSNLSFLTSKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKS 446
           TSM+GKK+NL  L SKLW+VGVT+VKTTEFVQGQT RWGLAWSFMP ARKII+P V  KS
Sbjct: 305 TSMLGKKANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKS 364

Query: 447 VLSFMLEGLLPQVSAMSILQSVELFLST 467
           VLSFMLE +  Q SA+ +LQSVE F  +
Sbjct: 365 VLSFMLECIKRQYSAVDVLQSVEEFFKS 376

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q554C95.9e-5838.76U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Dictyostelium disc... [more]
Q6DC647.2e-5639.82RNA N6-adenosine-methyltransferase mettl16 OS=Danio rerio OX=7955 GN=mettl16 PE=... [more]
Q6GR371.0e-5438.06RNA N6-adenosine-methyltransferase mettl16 OS=Xenopus laevis OX=8355 GN=mettl16 ... [more]
Q9CQG21.4e-5437.01RNA N6-adenosine-methyltransferase METTL16 OS=Mus musculus OX=10090 GN=Mettl16 P... [more]
Q86W503.0e-5438.89RNA N6-adenosine-methyltransferase METTL16 OS=Homo sapiens OX=9606 GN=METTL16 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1I1461.3e-25488.98U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita maxima O... [more]
A0A6J1C2I03.9e-25489.14U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Momordica charanti... [more]
A0A6J1GKM35.6e-25388.57U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita moschata... [more]
A0A1S3BIM52.4e-25188.66U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis melo OX=36... [more]
A0A0A0K5X12.9e-24987.84U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis sativus OX... [more]
Match NameE-valueIdentityDescription
XP_038886852.13.3e-25590.18U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase isoform X2 [Benincasa... [more]
XP_022969493.12.8e-25488.98U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita maxima][more]
XP_022135953.18.0e-25489.14methyltransferase-like protein 16 homolog isoform X1 [Momordica charantia] >XP_0... [more]
XP_022952458.11.2e-25288.57U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita moschata][more]
XP_023554274.14.4e-25288.37U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
AT2G21070.13.2e-16861.21methyltransferases [more]
AT2G21070.33.2e-16861.21methyltransferases [more]
AT2G21070.22.2e-14064.18methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017182Methyltransferase METTL16/PsiMPIRSFPIRSF037350Mtase_ZK1128coord: 84..571
e-value: 2.1E-132
score: 440.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 128..423
e-value: 1.1E-84
score: 285.8
NoneNo IPR availablePIRSRPIRSR037350-1PIRSR037350-1coord: 312..428
e-value: 1.3E-40
score: 137.2
coord: 128..259
e-value: 1.3E-42
score: 143.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
IPR010286METTL16/RlmF familyPFAMPF05971Methyltransf_10coord: 95..261
e-value: 1.8E-47
score: 162.2
coord: 293..423
e-value: 3.3E-31
score: 108.7
IPR010286METTL16/RlmF familyPANTHERPTHR13393SAM-DEPENDENT METHYLTRANSFERASEcoord: 86..558
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 154..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g014590.1Lcy05g014590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity