Lcy05g011890 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g011890
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	rRNA N-glycosidase
Location	Chr05: 13612902 .. 13613729 (+)
RNA-Seq Expression	Lcy05g011890
Synteny	Lcy05g011890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGATGAAGAAATTGGTTGGGTTTTGCTTTGTTGTGGCTCTCTATCTTGGTGCTCATACCACAGAGGCTAATGTCAGCTTCTCTCTATTGGGTGCCACTAGTGCAAGCTACAAACAGTTCATAAGCAATCTGCGCACCACACTCACGGTTGGAACTCCAATTGTGTACAATATACCTGTGTTGAGGAACACAGCAGCAGGGGCGGCGCGATACCTCTTAGTGCAGCTCACGAATTATAATCGTGAATCTATTACTATGGCACTGGATGTGGTGAACGTGTATCTTGTTGCATATCAAGCTGGAAATACTCGCTACTTTCTCAATGATTCGCCAGCAGGAGCGACCGATGTGCTATTTACAGGCTCCACTAGAACGGTAAGATTTTCATATGGAGGCAATTATGATGGCCTTGAGAGGGCTGCAGGGACAAGTAGAGAGAATATTGACCTTGGATTTTCCGTGATGAGTAGTTCCATTGGTAGCATGTTTCGCAACGATGTCGGTACTGCTGTCCCTAGAGCATTTATTGTCATCATCCAAACGATCTCTGAAGCTGCAAGATTTAAATACATTGAGCAAAGAGTTTCTGAAAGTGTTCAAACCACGTTTCGGCCCGACCCTGCATTTTTAAGCTTACAAAATAACTGGGGCAGTCTCTCTGAACAAATACAGATTGCTCAATTTCAAGGAGGGAAGTTTTCTCGTGCTGTCGAGCTTCGAACGGTTAGCAACACTCCAATTTTGGTGGACAATGTTAATTCACCTGTTGTGAGAGGGATTGCACTTCTAAAATACTTCAATGTTAGTGTTCCCATGCATTGA

mRNA sequence

ATGGAAAAGATGAAGAAATTGGTTGGGTTTTGCTTTGTTGTGGCTCTCTATCTTGGTGCTCATACCACAGAGGCTAATGTCAGCTTCTCTCTATTGGGTGCCACTAGTGCAAGCTACAAACAGTTCATAAGCAATCTGCGCACCACACTCACGGTTGGAACTCCAATTGTGTACAATATACCTGTGTTGAGGAACACAGCAGCAGGGGCGGCGCGATACCTCTTAGTGCAGCTCACGAATTATAATCGTGAATCTATTACTATGGCACTGGATGTGGTGAACGTGTATCTTGTTGCATATCAAGCTGGAAATACTCGCTACTTTCTCAATGATTCGCCAGCAGGAGCGACCGATGTGCTATTTACAGGCTCCACTAGAACGGTAAGATTTTCATATGGAGGCAATTATGATGGCCTTGAGAGGGCTGCAGGGACAAGTAGAGAGAATATTGACCTTGGATTTTCCGTGATGAGTAGTTCCATTGGTAGCATGTTTCGCAACGATGTCGGTACTGCTGTCCCTAGAGCATTTATTGTCATCATCCAAACGATCTCTGAAGCTGCAAGATTTAAATACATTGAGCAAAGAGTTTCTGAAAGTGTTCAAACCACGTTTCGGCCCGACCCTGCATTTTTAAGCTTACAAAATAACTGGGGCAGTCTCTCTGAACAAATACAGATTGCTCAATTTCAAGGAGGGAAGTTTTCTCGTGCTGTCGAGCTTCGAACGGTTAGCAACACTCCAATTTTGGTGGACAATGTTAATTCACCTGTTGTGAGAGGGATTGCACTTCTAAAATACTTCAATGTTAGTGTTCCCATGCATTGA

Coding sequence (CDS)

ATGGAAAAGATGAAGAAATTGGTTGGGTTTTGCTTTGTTGTGGCTCTCTATCTTGGTGCTCATACCACAGAGGCTAATGTCAGCTTCTCTCTATTGGGTGCCACTAGTGCAAGCTACAAACAGTTCATAAGCAATCTGCGCACCACACTCACGGTTGGAACTCCAATTGTGTACAATATACCTGTGTTGAGGAACACAGCAGCAGGGGCGGCGCGATACCTCTTAGTGCAGCTCACGAATTATAATCGTGAATCTATTACTATGGCACTGGATGTGGTGAACGTGTATCTTGTTGCATATCAAGCTGGAAATACTCGCTACTTTCTCAATGATTCGCCAGCAGGAGCGACCGATGTGCTATTTACAGGCTCCACTAGAACGGTAAGATTTTCATATGGAGGCAATTATGATGGCCTTGAGAGGGCTGCAGGGACAAGTAGAGAGAATATTGACCTTGGATTTTCCGTGATGAGTAGTTCCATTGGTAGCATGTTTCGCAACGATGTCGGTACTGCTGTCCCTAGAGCATTTATTGTCATCATCCAAACGATCTCTGAAGCTGCAAGATTTAAATACATTGAGCAAAGAGTTTCTGAAAGTGTTCAAACCACGTTTCGGCCCGACCCTGCATTTTTAAGCTTACAAAATAACTGGGGCAGTCTCTCTGAACAAATACAGATTGCTCAATTTCAAGGAGGGAAGTTTTCTCGTGCTGTCGAGCTTCGAACGGTTAGCAACACTCCAATTTTGGTGGACAATGTTAATTCACCTGTTGTGAGAGGGATTGCACTTCTAAAATACTTCAATGTTAGTGTTCCCATGCATTGA

Protein sequence

MEKMKKLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNTAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTRTVRFSYGGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTISEAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVSNTPILVDNVNSPVVRGIALLKYFNVSVPMH

Homology

[Close]

BLAST of Lcy05g011890 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.7e-98
Identity = 193/267 (72.28%), Postives = 224/267 (83.90%), Query Frame = 0


Query: 8   VGFCFVVALYLGAHTTE-ANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNT 67
           +GF  V+ALY+GAH TE  +++FSL+GAT A+YK FI NLRT LTVGTP VY+IPVLRN 
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 68  AAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTR 127
           AAG AR+ LV LTNYN ES+T+ALDVVNVY+VAY+AGNT YFL D+   A +VLF G   
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 128 TVRFSYGGNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTIS 187
            VR  YGGNYDGLE AAG  SRENI+LGFS +SS+IG+MFR++ GT+VPRAFIVIIQT+S
Sbjct: 124 -VRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVS 183

Query: 188 EAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVS 247
           EAARFKYIEQRVSE+V T F+PDPAFLSLQN WGSLSEQIQIAQ +GG+F+R VELRTVS
Sbjct: 184 EAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVS 243

Query: 248 NTPILVDNVNSPVVRGIALLKYFNVSV 273
           NTP  V NVNSPVV+GIALL YF V+V
Sbjct: 244 NTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of Lcy05g011890 vs. ExPASy Swiss-Prot
Match: Q5F0I3 (Ribosome-inactivating protein gynostemmin OS=Gynostemma pentaphyllum OX=182084 PE=1 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 4.4e-51
Identity = 121/272 (44.49%), Postives = 174/272 (63.97%), Query Frame = 0


Query: 6   KLVGFCFVVA--LYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVL 65
           ++  FC VVA  LY G H  E +++FSL GA   +Y  FI+ LR  L++GT  V NI VL
Sbjct: 2   RVARFCTVVAILLYFGFHIAECDINFSLAGADGQTYNTFIAKLRQELSIGTQKVANITVL 61

Query: 66  R-NTAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSP--AGATDVL 125
           + + ++   R+L + L NYN + IT+ +DV +VY+V +  G   Y   ++P  A    +L
Sbjct: 62  KHHVSSNTQRFLSINLFNYNGDKITLGIDVFDVYVVGFLTGTNSYIFKEAPDLAYNKSLL 121

Query: 126 FTGSTRTVRFSYGGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVI 185
           F GS R    SY G YD LER  G  RE+I LG   + ++I ++FR D  T+  R+FIVI
Sbjct: 122 FPGSVRE-NLSYTGGYDDLER-RGAGREDIPLGLLPLDTAITNLFRRD-STSFRRSFIVI 181

Query: 186 IQTISEAARFKYIEQRVSESV--QTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRA 245
           IQ +SEAARFK IE ++++++  + TF+PD A +SL+NNWG+LS+QIQ AQ +GG F   
Sbjct: 182 IQMVSEAARFKIIEAKIAKNLYGENTFKPDQAIISLENNWGALSKQIQKAQDRGGVFPNL 241

Query: 246 VELRTVSNTPILVDNVNSPVVR-GIALLKYFN 270
           V L T S  P+++ N + P+V+ GIALLKY +
Sbjct: 242 VTLTTSSGKPLIIRNDSDPLVQNGIALLKYMS 270

[Close]

BLAST of Lcy05g011890 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.3e-50
Identity = 110/249 (44.18%), Postives = 167/249 (67.07%), Query Frame = 0


Query: 13  VVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNTAAGAAR 72
           ++A+++G  T + +V+F L  AT+ +Y +FI + R TL      VY+IP+L +T + + R
Sbjct: 11  IIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHK-VYDIPLLYSTISDSRR 70

Query: 73  YLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTRTVRFSY 132
           ++L+ LT+Y  E+I++A+DV NVY+VAY+  +  YF  +SP  A ++LF G TR +   Y
Sbjct: 71  FILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG-TRKITLPY 130

Query: 133 GGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTISEAARFKY 192
            GNY+ L+ AA   RENIDLG   +SS+I ++F  +  +A P A +V+IQT +EAARFKY
Sbjct: 131 TGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSA-PSALLVLIQTTAEAARFKY 190

Query: 193 IEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVSNTPILVD 252
           IE+ V++ V T F+P+ A +SL+N W +LS+QI +AQ QGGKF   V+L   +     V 
Sbjct: 191 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVT 250

Query: 253 NVNSPVVRG 262
           NV+S VV+G
Sbjct: 251 NVDSDVVKG 256

[Close]

BLAST of Lcy05g011890 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 1.3e-50
Identity = 110/249 (44.18%), Postives = 167/249 (67.07%), Query Frame = 0


Query: 13  VVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNTAAGAAR 72
           ++A+++G  T + +V+F L  AT+ +Y +FI + R TL      VY+IP+L +T + + R
Sbjct: 11  IIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHK-VYDIPLLYSTISDSRR 70

Query: 73  YLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTRTVRFSY 132
           ++L+ LT+Y  E+I++A+DV NVY+VAY+  +  YF  +SP  A ++LF G TR +   Y
Sbjct: 71  FILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG-TRKITLPY 130

Query: 133 GGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTISEAARFKY 192
            GNY+ L+ AA   RENIDLG   +SS+I ++F  +  +A P A +V+IQT +EAARFKY
Sbjct: 131 TGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSA-PSALLVLIQTTAEAARFKY 190

Query: 193 IEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVSNTPILVD 252
           IE+ V++ V T F+P+ A +SL+N W +LS+QI +AQ QGGKF   V+L   +     V 
Sbjct: 191 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVT 250

Query: 253 NVNSPVVRG 262
           NV+S VV+G
Sbjct: 251 NVDSDVVKG 256

[Close]

BLAST of Lcy05g011890 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 1.4e-49
Identity = 117/261 (44.83%), Postives = 166/261 (63.60%), Query Frame = 0


Query: 7   LVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNT 66
           LV    ++ L+L A   E +VSF L GATS+SY  FISNLR  L     + Y+IP+LR+T
Sbjct: 5   LVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKL-YDIPLLRST 64

Query: 67  AAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPA-GATDVLFTGST 126
             G+ RY L+ LTNY  E+I++A+DV NVY++ Y+AG+T YF N++ A  A   +F  + 
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAK 124

Query: 127 RTVRFSYGGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTIS 186
           R V   Y GNY+ L+ AAG  RENI LG   + S+I ++F  +  +A   A +V+IQ+ S
Sbjct: 125 RKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAA-SALMVLIQSTS 184

Query: 187 EAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVS 246
           EAAR+K+IEQ++ + V  TF P  A +SL+N+W +LS+QIQIA    G+F   V L    
Sbjct: 185 EAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQ 244

Query: 247 NTPILVDNVNSPVV-RGIALL 266
           N  + + NV++ VV   IALL
Sbjct: 245 NQRVTITNVDAGVVTSNIALL 263

[Close]

BLAST of Lcy05g011890 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 2.1e-96
Identity = 191/268 (71.27%), Postives = 224/268 (83.58%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRN 65
           KLVGF  V+ALY+GA  TEA+V+FSLLG+TSA+YKQFI NLR  LTVG+P V NIPVL+ 
Sbjct: 2   KLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKG 61

Query: 66  TAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGST 125
           TAAG AR+LLV LTNYN ESIT+A+DVVNVY+VAY+AGN  YFLND+ A A +VLF G  
Sbjct: 62  TAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGIN 121

Query: 126 RTVRFSYGGNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTI 185
             VR  YGGNYDGLE AAG  SRE I LGFS +SS+IGSMF  + GT+VP+AFIVIIQT+
Sbjct: 122 H-VRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 181

Query: 186 SEAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTV 245
           SEAARFKYI+QRVSE+VQT F PDPAFLSL+N WG LSEQIQIAQ++GG+F+R +ELRTV
Sbjct: 182 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 241

Query: 246 SNTPILVDNVNSPVVRGIALLKYFNVSV 273
           SNTP  V NVNSPVV+G+ALL Y+ V+V
Sbjct: 242 SNTPTFVTNVNSPVVKGVALLLYYKVNV 268

[Close]

BLAST of Lcy05g011890 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.1e-94
Identity = 188/269 (69.89%), Postives = 225/269 (83.64%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRN 65
           +L+ F F +ALYLGA   EA+VSFS+LG+TS +YKQFI N+R+ LTVG+ IVY+IPVL+ 
Sbjct: 2   RLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKA 61

Query: 66  TAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGST 125
           TA G+AR+LLV LTNYN ESI +A+DVVNVY+VAY+AGN  YFLND+ A A  VLF G  
Sbjct: 62  TATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGIN 121

Query: 126 RTVRFSYGGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTIS 185
             V+ SY GNYDGLE AA  SRE IDLGFS +SSSIG+MF ++ GT+VPRAFIVIIQ++S
Sbjct: 122 H-VKLSYKGNYDGLETAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVS 181

Query: 186 EAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVS 245
           EAARFKYIEQRVSE+V+TTF+PDPAFLSLQN W  LSEQIQIAQ +GG+F+RAVELRTVS
Sbjct: 182 EAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVS 241

Query: 246 NTPILVDNVNSPVVRGIALLKYFNVSVPM 275
           N PILV +V+SPVV+GIALL Y+ VSV M
Sbjct: 242 NKPILVTDVSSPVVKGIALLLYYKVSVVM 269

[Close]

BLAST of Lcy05g011890 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 8.5e-90
Identity = 177/268 (66.04%), Postives = 222/268 (82.84%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRN 65
           + + F FV+AL  GA   EA+VSFS+LGATS +Y+QFI NLR+TLT+ +P+VY IPVL++
Sbjct: 2   RFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQS 61

Query: 66  TAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGST 125
           TA G+AR++LV LTNYN ESIT+A+DVVN+Y+VAY+AGN  YFLND+ A A +VLF G  
Sbjct: 62  TAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKG-I 121

Query: 126 RTVRFSYGGNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTI 185
           + VR  Y GNYDGLE AAG  SRE IDLGFS +SSSIG+MF ++VGT++ RAFIVIIQ++
Sbjct: 122 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 181

Query: 186 SEAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTV 245
            EAARFKYIEQRVSE+V+T F+PDPAFLSL+N W  LSEQIQIAQ + G+F+RA+ELRTV
Sbjct: 182 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 241

Query: 246 SNTPILVDNVNSPVVRGIALLKYFNVSV 273
           SN PI+V NV+SP+V+GIALL Y+ V V
Sbjct: 242 SNKPIIVTNVSSPIVKGIALLLYYKVRV 268

[Close]

BLAST of Lcy05g011890 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 4.1e-76
Identity = 154/244 (63.11%), Postives = 191/244 (78.28%), Query Frame = 0


Query: 25  ANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNTAAGAARYLLVQLTNYNRE 84
           +NVSFSLLGAT  +Y+QFI NLR  LT+ + IVYNIPVL  TA  +AR++LV LTNY  E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 85  SITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTRTVRFSYGGNYDGLERAAG 144
           +IT+A+DVVNVY+VAYQAGN  YFL D+   A DVLF G  + +   Y GNYDGLE AAG
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEI-LPYKGNYDGLETAAG 154

Query: 145 -TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTISEAARFKYIEQRVSESVQT 204
             SRE IDLGFS + S+IG+M+  + GT+VPRAFIV+IQTISEAARFKYIE +VS++V+T
Sbjct: 155 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 214

Query: 205 TFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVSNTPILVDNVNSPVVRGIA 264
            F+PDP FLSL+N W  LSEQ+QIAQ + G+F+R +E+R+V+N PILV NV S VV G+A
Sbjct: 215 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 274

Query: 265 LLKY 268
           LL Y
Sbjct: 275 LLLY 277

[Close]

BLAST of Lcy05g011890 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.6e-72
Identity = 153/253 (60.47%), Postives = 191/253 (75.49%), Query Frame = 0


Query: 14  VALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNTAAGAARY 73
           VA+     +  AN+SFSLLGAT+ +YKQFIS+LR  LTV +  VY+IPVL  TA G AR+
Sbjct: 23  VAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATAKGLARF 82

Query: 74  LLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTRTVRFSYG 133
           +LV LTNY  ESIT+A+D V+VY+VAYQAGN  YFL D    A DVLF GS      SY 
Sbjct: 83  ILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHET-LSYK 142

Query: 134 GNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTISEAARFKY 193
           GNYD LER AG  SRENIDLGFS +SSSIG+MF  + G +VP++FIV+IQ +SEA+RFKY
Sbjct: 143 GNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEASRFKY 202

Query: 194 IEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVSNTPILVD 253
           IE R  E+V++ F+PDP +LSL+N W +LSEQ+Q AQ  GGKF   V++R+VSN P+LV 
Sbjct: 203 IEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVS 262

Query: 254 NVNSPVVRGIALL 266
           +VNSPVV+G+ALL
Sbjct: 263 DVNSPVVKGMALL 274

[Close]

BLAST of Lcy05g011890 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 362.1 bits (928), Expect = 4.3e-96
Identity = 191/268 (71.27%), Postives = 224/268 (83.58%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRN 65
           KLVGF  V+ALY+GA  TEA+V+FSLLG+TSA+YKQFI NLR  LTVG+P V NIPVL+ 
Sbjct: 2   KLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKG 61

Query: 66  TAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGST 125
           TAAG AR+LLV LTNYN ESIT+A+DVVNVY+VAY+AGN  YFLND+ A A +VLF G  
Sbjct: 62  TAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGIN 121

Query: 126 RTVRFSYGGNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTI 185
             VR  YGGNYDGLE AAG  SRE I LGFS +SS+IGSMF  + GT+VP+AFIVIIQT+
Sbjct: 122 H-VRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 181

Query: 186 SEAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTV 245
           SEAARFKYI+QRVSE+VQT F PDPAFLSL+N WG LSEQIQIAQ++GG+F+R +ELRTV
Sbjct: 182 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 241

Query: 246 SNTPILVDNVNSPVVRGIALLKYFNVSV 273
           SNTP  V NVNSPVV+G+ALL Y+ V+V
Sbjct: 242 SNTPTFVTNVNSPVVKGVALLLYYKVNV 268

[Close]

BLAST of Lcy05g011890 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 360.5 bits (924), Expect = 1.3e-95
Identity = 193/267 (72.28%), Postives = 224/267 (83.90%), Query Frame = 0


Query: 8   VGFCFVVALYLGAHTTE-ANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNT 67
           +GF  V+ALY+GAH TE  +++FSL+GAT A+YK FI NLRT LTVGTP VY+IPVLRN 
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 68  AAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTR 127
           AAG AR+ LV LTNYN ES+T+ALDVVNVY+VAY+AGNT YFL D+   A +VLF G   
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 128 TVRFSYGGNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTIS 187
            VR  YGGNYDGLE AAG  SRENI+LGFS +SS+IG+MFR++ GT+VPRAFIVIIQT+S
Sbjct: 124 -VRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVS 183

Query: 188 EAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVS 247
           EAARFKYIEQRVSE+V T F+PDPAFLSLQN WGSLSEQIQIAQ +GG+F+R VELRTVS
Sbjct: 184 EAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVS 243

Query: 248 NTPILVDNVNSPVVRGIALLKYFNVSV 273
           NTP  V NVNSPVV+GIALL YF V+V
Sbjct: 244 NTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of Lcy05g011890 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 356.3 bits (913), Expect = 2.4e-94
Identity = 188/269 (69.89%), Postives = 225/269 (83.64%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRN 65
           +L+ F F +ALYLGA   EA+VSFS+LG+TS +YKQFI N+R+ LTVG+ IVY+IPVL+ 
Sbjct: 2   RLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKA 61

Query: 66  TAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGST 125
           TA G+AR+LLV LTNYN ESI +A+DVVNVY+VAY+AGN  YFLND+ A A  VLF G  
Sbjct: 62  TATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGIN 121

Query: 126 RTVRFSYGGNYDGLERAAGTSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTIS 185
             V+ SY GNYDGLE AA  SRE IDLGFS +SSSIG+MF ++ GT+VPRAFIVIIQ++S
Sbjct: 122 H-VKLSYKGNYDGLETAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVS 181

Query: 186 EAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVS 245
           EAARFKYIEQRVSE+V+TTF+PDPAFLSLQN W  LSEQIQIAQ +GG+F+RAVELRTVS
Sbjct: 182 EAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVS 241

Query: 246 NTPILVDNVNSPVVRGIALLKYFNVSVPM 275
           N PILV +V+SPVV+GIALL Y+ VSV M
Sbjct: 242 NKPILVTDVSSPVVKGIALLLYYKVSVVM 269

[Close]

BLAST of Lcy05g011890 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 340.1 bits (871), Expect = 1.8e-89
Identity = 177/268 (66.04%), Postives = 222/268 (82.84%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRN 65
           + + F FV+AL  GA   EA+VSFS+LGATS +Y+QFI NLR+TLT+ +P+VY IPVL++
Sbjct: 2   RFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQS 61

Query: 66  TAAGAARYLLVQLTNYNRESITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGST 125
           TA G+AR++LV LTNYN ESIT+A+DVVN+Y+VAY+AGN  YFLND+ A A +VLF G  
Sbjct: 62  TAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKG-I 121

Query: 126 RTVRFSYGGNYDGLERAAG-TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTI 185
           + VR  Y GNYDGLE AAG  SRE IDLGFS +SSSIG+MF ++VGT++ RAFIVIIQ++
Sbjct: 122 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 181

Query: 186 SEAARFKYIEQRVSESVQTTFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTV 245
            EAARFKYIEQRVSE+V+T F+PDPAFLSL+N W  LSEQIQIAQ + G+F+RA+ELRTV
Sbjct: 182 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 241

Query: 246 SNTPILVDNVNSPVVRGIALLKYFNVSV 273
           SN PI+V NV+SP+V+GIALL Y+ V V
Sbjct: 242 SNKPIIVTNVSSPIVKGIALLLYYKVRV 268

[Close]

BLAST of Lcy05g011890 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 294.7 bits (753), Expect = 8.5e-76
Identity = 154/244 (63.11%), Postives = 191/244 (78.28%), Query Frame = 0


Query: 25  ANVSFSLLGATSASYKQFISNLRTTLTVGTPIVYNIPVLRNTAAGAARYLLVQLTNYNRE 84
           +NVSFSLLGAT  +Y+QFI NLR  LT+ + IVYNIPVL  TA  +AR++LV LTNY  E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 85  SITMALDVVNVYLVAYQAGNTRYFLNDSPAGATDVLFTGSTRTVRFSYGGNYDGLERAAG 144
           +IT+A+DVVNVY+VAYQAGN  YFL D+   A DVLF G  + +   Y GNYDGLE AAG
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEI-LPYKGNYDGLETAAG 154

Query: 145 -TSRENIDLGFSVMSSSIGSMFRNDVGTAVPRAFIVIIQTISEAARFKYIEQRVSESVQT 204
             SRE IDLGFS + S+IG+M+  + GT+VPRAFIV+IQTISEAARFKYIE +VS++V+T
Sbjct: 155 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 214

Query: 205 TFRPDPAFLSLQNNWGSLSEQIQIAQFQGGKFSRAVELRTVSNTPILVDNVNSPVVRGIA 264
            F+PDP FLSL+N W  LSEQ+QIAQ + G+F+R +E+R+V+N PILV NV S VV G+A
Sbjct: 215 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 274

Query: 265 LLKY 268
           LL Y
Sbjct: 275 LLLY 277

The following BLAST results are available for this feature:

BLAST of Lcy05g011890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.7e-98	72.28	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
Q5F0I3	4.4e-51	44.49	Ribosome-inactivating protein gynostemmin OS=Gynostemma pentaphyllum OX=182084 P...	[more]
P29339	1.3e-50	44.18	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P24817	1.3e-50	44.18	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P24478	1.4e-49	44.83	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]

BLAST of Lcy05g011890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B1B626	2.1e-96	71.27	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
A0A6J1DVE7	1.1e-94	69.89	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
A0A6J1DWK4	8.5e-90	66.04	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1E093	4.1e-76	63.11	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1IUB2	1.6e-72	60.47	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]

BLAST of Lcy05g011890 vs. NCBI nr


Match Name	E-value	Identity	Description
BAG12408.1	4.3e-96	71.27	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
P98184.1	1.3e-95	72.28	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
XP_022158193.1	2.4e-94	69.89	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158192.1	1.8e-89	66.04	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158191.1	8.5e-76	63.11	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of Lcy05g011890 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 37..50 score: 42.48 coord: 177..197 score: 60.28 coord: 85..100 score: 50.49 coord: 147..161 score: 42.11 coord: 209..226 score: 45.47
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 26..189 e-value: 1.5E-43 score: 150.4
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 191..274 e-value: 8.2E-27 score: 95.5
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 35..226 e-value: 1.6E-32 score: 112.8
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 21..267
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 21..267
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 181..197
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 27..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g011890.1	Lcy05g011890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity