Lcy04g017650 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g017650
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptiontransmembrane protein 53
LocationChr04: 49666729 .. 49669252 (-)
RNA-Seq ExpressionLcy04g017650
SyntenyLcy04g017650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCGAGCTTCGAGCCTCTAATCCAGCTTTCCGGGCAAAATCCCTCTCCGACTCCGGCGAAAGATTCAGAAACTGGCTTTGTTTCTGGCCGGAAAATTTGGAGGAGCAAAACCAAATCTTCTCAAATTGGTCGCTGGGAGTGCTTCCATGGAAGCTCCAGTAAGGGCATTTCGGCCCTACGTTCTTAATCGCCGCCTGTTTTCAAAAACAATTCCTCCATCGCGCCTCGTTGCATCTCCTGAATCTAATCGGAGCGCACTTTTCCGGCAATCTCCAACCTATTTGCATCCTCCTCCATCTCGAACGTTCCCCGGTTCCAAAATCTCACTCTCACTCAACCTTTCTCACTGCTCCTCCTCGCCCTTCGGATCTTCTTCCTCTTCTCTTGGCTTTAACCCTTCTAATTTTCTTTCTTCTTTACCCTCAATACAATCTTTCGGCTCCCAATTCGAGCCTGATCACTTCAATTCGTTCCTATCCGATTCGAATGGCAGTACCTGGACTTGGAATCGAGCTTCAGAGAGTGCAATTAAGGGAAACGTGGGGGTTTTAGGAGGCGAAAAGGGATCGGCGACCGTTGTGTTGTTAGGTTGGCTCGGGTCGAAGACAAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCAAGGGGTATCAACGCCTTGACGTTTGTGGTTGATCCGACCGAGTTTCTTTGGTTTGCGTTGAGTCGGAGAGTTGAACAGCGGATTTCGGATTTAGCGGTTGAACTCATTTCTTGGTTATCAGACGGAGAAGGGAGTGATAAAGATCGGTCCCTGATATTTCACACTTTTAGCAATACAGGCTGGTTTGTGTAAGTTGAATGATGTTTTCAGTATCTCCTTGGAGTTGTTTTGAGATTTCAACTAGAATTAGCGTTGTTTTCCTTGTCCCTTTTCAGATACGGCGCCATTCTTGAAATTTTGCAGGGTCGGAAGGATTTGTTGGAGAAGATCAAAGGATGCATTGTTGATTCTGGAGGAGGTGATCCATTGAATCCTCAGGTTTGTGAACTGATAGTTATATGATTTTGGAACTGCTGCACGATAATATACATATATATACGATCGTTCTTTGAACTGTTGGAAAAACAACATCTGCATAACAAATGGCGAGAATGGAAGGATTTGGTGGTGAAAATTTAGTAGGAACAGGTTATTGCTCTTCAAATATCATATGAATCTCAATCTCTATCTGTCCCTTTGATGCTCTTTCTTTTTCTCTCTGTTCCCTCTTTCTTTCTTTAGTTCCAAATTGTTTGAATCAGATTGTATTGACTGCAACTGGGAGTTTTCTTATTGATTACAATTATATTTACCCAATATCTCATTTAAAATTTCCAACTCCCAGTTTCCCAAGGCTTCTAAATAGTCCACAAAGTTTGGTTAAAAGTTTTCTTGTCATAAACTATTGAAGCATATGCAAGCAACTAGTTAGATTGTTTAAGCAATAAGATTAATAACGTAATAGCTAGCATGCTTATATCCAATCTGATTTAGATAGCATGCTTTTGTGTCATCTTAACATACATTGTTATCAATTTTTCTGCCTTCCTCGTCTGCTTTACAAATAAGATATCAACTACCATGCTTTCATCTAATCTGTTTTGCTTTCCACTTCATTCCCATTCAAAGTGGAAGAGAGAAATTTGATTTCATGGCTACTACGACGCTGACAGGTTTGGGCAGCTGGATTTTCTGCAGCCATTCTCAAGAAAAATAGTTCTTCTACATCTCCTGTCGTTGATGGAGAGGAATTAGACAAGAAACCTCCTTGGATGGAAACCTTTGTTCTATCATCATTGGAGAAGTTTTTCTCAGTCGCTTTGAAGTTGCCAGAAGTCGATAAGTGAGTTTTCTAATTATATCCAGACATGGATCCATATTCACTAGAAGTTCGAATGACCATATACTTATCGTATGGATGCATATCAATTGTTGAGAAATGAACATTTCCTTGCAGGAGATTGAAGAAGATTGTTTCAGTTCTTACAGAGAACCAGCCTTTATATCCTGAGCTTTATCTGTACAGTTCAGGAGATAGAGTTGTGCCATTCGAGTCAATCGAGCTACTTATCGAGAAGAGAAAGAAGACGGGAAGGAAGGTTTTGTCACACGACTTTCGCTCATCGCCGCATGTTGATCATTACAGGACATACCCAGATATATACTCATCACAGCTTCACAAGTTCTTGGTAGAGTCTTTTTCTTCATAGGTTGCTGTGGATTGGTGCATATGGCAGATAATTTGTGCTGAAGATTTAGAAGATAATGAGATTATTTCATTAGGGCAATAATTGGGTTAACTAAAGCTTCATCTTGCAGAGACTACAAATAGTGCTCATACATAAATACATTTATTTCAGACAATTCAAATAGGGTGAAGAGAAACTTTCAATTTGAGAGAGCATTTTTCAAATGTATCTTTTTAGTAATAATTGACAAGTATTGAAAATAACATAATGTCATTGTTGCCCAAATAGAATAAAATATCTATACTCTCTAC

mRNA sequence

CTTCGAGCTTCGAGCCTCTAATCCAGCTTTCCGGGCAAAATCCCTCTCCGACTCCGGCGAAAGATTCAGAAACTGGCTTTGTTTCTGGCCGGAAAATTTGGAGGAGCAAAACCAAATCTTCTCAAATTGGTCGCTGGGAGTGCTTCCATGGAAGCTCCAGTAAGGGCATTTCGGCCCTACGTTCTTAATCGCCGCCTGTTTTCAAAAACAATTCCTCCATCGCGCCTCGTTGCATCTCCTGAATCTAATCGGAGCGCACTTTTCCGGCAATCTCCAACCTATTTGCATCCTCCTCCATCTCGAACGTTCCCCGGTTCCAAAATCTCACTCTCACTCAACCTTTCTCACTGCTCCTCCTCGCCCTTCGGATCTTCTTCCTCTTCTCTTGGCTTTAACCCTTCTAATTTTCTTTCTTCTTTACCCTCAATACAATCTTTCGGCTCCCAATTCGAGCCTGATCACTTCAATTCGTTCCTATCCGATTCGAATGGCAGTACCTGGACTTGGAATCGAGCTTCAGAGAGTGCAATTAAGGGAAACGTGGGGGTTTTAGGAGGCGAAAAGGGATCGGCGACCGTTGTGTTGTTAGGTTGGCTCGGGTCGAAGACAAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCAAGGGGTATCAACGCCTTGACGTTTGTGGTTGATCCGACCGAGTTTCTTTGGTTTGCGTTGAGTCGGAGAGTTGAACAGCGGATTTCGGATTTAGCGGTTGAACTCATTTCTTGGTTATCAGACGGAGAAGGGAGTGATAAAGATCGGTCCCTGATATTTCACACTTTTAGCAATACAGGCTGGTTTGTATACGGCGCCATTCTTGAAATTTTGCAGGGTCGGAAGGATTTGTTGGAGAAGATCAAAGGATGCATTGTTGATTCTGGAGGAGGTGATCCATTGAATCCTCAGGTTTGGGCAGCTGGATTTTCTGCAGCCATTCTCAAGAAAAATAGTTCTTCTACATCTCCTGTCGTTGATGGAGAGGAATTAGACAAGAAACCTCCTTGGATGGAAACCTTTGTTCTATCATCATTGGAGAAGTTTTTCTCAGTCGCTTTGAAGTTGCCAGAAGTCGATAAGAGATTGAAGAAGATTGTTTCAGTTCTTACAGAGAACCAGCCTTTATATCCTGAGCTTTATCTGTACAGTTCAGGAGATAGAGTTGTGCCATTCGAGTCAATCGAGCTACTTATCGAGAAGAGAAAGAAGACGGGAAGGAAGGTTTTGTCACACGACTTTCGCTCATCGCCGCATGTTGATCATTACAGGACATACCCAGATATATACTCATCACAGCTTCACAAGTTCTTGGTAGAGTCTTTTTCTTCATAGGTTGCTGTGGATTGGTGCATATGGCAGATAATTTGTGCTGAAGATTTAGAAGATAATGAGATTATTTCATTAGGGCAATAATTGGGTTAACTAAAGCTTCATCTTGCAGAGACTACAAATAGTGCTCATACATAAATACATTTATTTCAGACAATTCAAATAGGGTGAAGAGAAACTTTCAATTTGAGAGAGCATTTTTCAAATGTATCTTTTTAGTAATAATTGACAAGTATTGAAAATAACATAATGTCATTGTTGCCCAAATAGAATAAAATATCTATACTCTCTAC

Coding sequence (CDS)

ATGGAAGCTCCAGTAAGGGCATTTCGGCCCTACGTTCTTAATCGCCGCCTGTTTTCAAAAACAATTCCTCCATCGCGCCTCGTTGCATCTCCTGAATCTAATCGGAGCGCACTTTTCCGGCAATCTCCAACCTATTTGCATCCTCCTCCATCTCGAACGTTCCCCGGTTCCAAAATCTCACTCTCACTCAACCTTTCTCACTGCTCCTCCTCGCCCTTCGGATCTTCTTCCTCTTCTCTTGGCTTTAACCCTTCTAATTTTCTTTCTTCTTTACCCTCAATACAATCTTTCGGCTCCCAATTCGAGCCTGATCACTTCAATTCGTTCCTATCCGATTCGAATGGCAGTACCTGGACTTGGAATCGAGCTTCAGAGAGTGCAATTAAGGGAAACGTGGGGGTTTTAGGAGGCGAAAAGGGATCGGCGACCGTTGTGTTGTTAGGTTGGCTCGGGTCGAAGACAAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCAAGGGGTATCAACGCCTTGACGTTTGTGGTTGATCCGACCGAGTTTCTTTGGTTTGCGTTGAGTCGGAGAGTTGAACAGCGGATTTCGGATTTAGCGGTTGAACTCATTTCTTGGTTATCAGACGGAGAAGGGAGTGATAAAGATCGGTCCCTGATATTTCACACTTTTAGCAATACAGGCTGGTTTGTATACGGCGCCATTCTTGAAATTTTGCAGGGTCGGAAGGATTTGTTGGAGAAGATCAAAGGATGCATTGTTGATTCTGGAGGAGGTGATCCATTGAATCCTCAGGTTTGGGCAGCTGGATTTTCTGCAGCCATTCTCAAGAAAAATAGTTCTTCTACATCTCCTGTCGTTGATGGAGAGGAATTAGACAAGAAACCTCCTTGGATGGAAACCTTTGTTCTATCATCATTGGAGAAGTTTTTCTCAGTCGCTTTGAAGTTGCCAGAAGTCGATAAGAGATTGAAGAAGATTGTTTCAGTTCTTACAGAGAACCAGCCTTTATATCCTGAGCTTTATCTGTACAGTTCAGGAGATAGAGTTGTGCCATTCGAGTCAATCGAGCTACTTATCGAGAAGAGAAAGAAGACGGGAAGGAAGGTTTTGTCACACGACTTTCGCTCATCGCCGCATGTTGATCATTACAGGACATACCCAGATATATACTCATCACAGCTTCACAAGTTCTTGGTAGAGTCTTTTTCTTCATAG

Protein sequence

MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKISLSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTWNRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETFVLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS
Homology
BLAST of Lcy04g017650 vs. ExPASy Swiss-Prot
Match: Q6P2H8 (Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 8.5e-19
Identity = 84/262 (32.06%), Postives = 120/262 (45.80%), Query Frame = 0

Query: 138 EKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDL 197
           E     V+LLGW G K K+L +Y   Y+ RG   + +   P   ++F+ S  +   +  L
Sbjct: 32  ETRQPVVILLGWGGCKDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSESLGIPS-LRVL 91

Query: 198 AVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEKIKGCIVDSGGG 257
           A +L+  L D E   +   L+FH FSN G  +Y  +LE+LQ R+    ++ G I DS  G
Sbjct: 92  AQKLLELLFDYE--IEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPG 151

Query: 258 DPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETFVLSSLEKFFSVALKLPE 317
           D  +  V A    AAIL++ ++    ++               V  +L       L  P 
Sbjct: 152 D--SNLVGALRALAAILERRAAMLRLLL--------------LVAFALVVVLFHVLLAPI 211

Query: 318 VDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVL--SHDFRS 377
                      L +    +PELYLYS  D VV    IE ++E R    R+VL  S DF S
Sbjct: 212 TALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEAR--LARRVLARSVDFVS 271

Query: 378 SPHVDHYRTYPDIYSSQLHKFL 398
           S HV H R YP  Y+S    F+
Sbjct: 272 SAHVSHLRDYPTYYTSLCVDFM 271

BLAST of Lcy04g017650 vs. ExPASy Swiss-Prot
Match: Q9D0Z3 (Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.1e-17
Identity = 77/260 (29.62%), Postives = 118/260 (45.38%), Query Frame = 0

Query: 139 KGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLA 198
           K    V+LLGW G + K+L +Y   Y+ RG   + +   P   ++F+ S  +   +  +A
Sbjct: 31  KQQPVVILLGWGGCRDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSESLGIPS-LRVIA 90

Query: 199 VELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLE-KIKGCIVDSGGG 258
            +L+  L D E   +   L+FH FSN G  +Y  +LE+LQ  +      + G I DSG G
Sbjct: 91  QKLLELLFDYE--IEREPLLFHVFSNAGVMLYRYVLELLQTHQRFRHLHVVGTIFDSGPG 150

Query: 259 DPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETFVLSSLEKFFSVALKLPE 318
           D  +  + A    A IL++  +    ++           +  F L  +   F +A   P 
Sbjct: 151 D--SNLIGALRALATILERRPAVLRLLL-----------LAAFALVVILFHFLLA---PF 210

Query: 319 VDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSP 378
                      L ++   +PELYLYS  D+VV    +E ++E R      V   DF SS 
Sbjct: 211 TALFHTHFYDRLQDSGSCWPELYLYSRADKVVSARDVERMVEARLAHQVMVRGVDFVSSA 270

Query: 379 HVDHYRTYPDIYSSQLHKFL 398
           HV H R YP  Y+S    F+
Sbjct: 271 HVSHLRDYPTYYTSLCVDFM 270

BLAST of Lcy04g017650 vs. ExPASy Swiss-Prot
Match: Q6DHN0 (Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.7e-17
Identity = 72/261 (27.59%), Postives = 126/261 (48.28%), Query Frame = 0

Query: 144 VVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLAVELIS 203
           V+LLGW GS+ KHL +Y   YN +G   L +   P + ++ + S   ++ +   A +L+ 
Sbjct: 30  VILLGWAGSRDKHLAKYSSIYNEQGCTTLRYTA-PLKTVFISESLGYKE-LRSTAHKLLE 89

Query: 204 WLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEK-IKGCIVDSGGGDPLNP 263
            L D E   ++  + FH FSN G+ +Y  ++E+L   K      + G +VDS  G   + 
Sbjct: 90  LLYDYE--VENNPIFFHVFSNGGFMLYRYMVELLHSHKQFSTLCVVGTVVDSAPG---SQ 149

Query: 264 QVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETFVLSSLEKFFSVALKL------P 323
            V  A      L+   ++  P V+          ++ F+L+     F+VA+ L      P
Sbjct: 150 NVVGA------LRALKTTLGPKVN--------VLLQYFLLA----LFAVAVFLLRIVLYP 209

Query: 324 EVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSS 383
                 +     + E+   +P++YLYS  DRV+ +  +E +++  ++ G  V S DF + 
Sbjct: 210 LTKYFHRNHYDAMMEHPAPWPQMYLYSRADRVIRYRDVEKMVKGLQEKGLMVESFDFITP 265

Query: 384 PHVDHYRTYPDIYSSQLHKFL 398
            HV  +R  P+ YS++   FL
Sbjct: 270 AHVSLFRDCPEDYSNRCRTFL 265

BLAST of Lcy04g017650 vs. ExPASy Swiss-Prot
Match: Q2TBP5 (Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 8.0e-17
Identity = 75/255 (29.41%), Postives = 114/255 (44.71%), Query Frame = 0

Query: 144 VVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLAVELIS 203
           V+LLGW G   K+L +Y   Y+ RG   + +   P   ++F+ +  +   +  LA +L+ 
Sbjct: 38  VILLGWGGCSDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSETLGIPS-LRVLAQKLLE 97

Query: 204 WLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLE-KIKGCIVDSGGGDPLNP 263
            L D E   +   L+FH FSN G  +Y  +LE+LQ  +     ++ G I DSG GD  + 
Sbjct: 98  LLFDYE--VEKEPLLFHVFSNAGVMLYRYVLELLQTHQRFCHLRVVGTIFDSGPGD--SN 157

Query: 264 QVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETFVLSSLEKFFSVALKLPEVDKRL 323
            + A    A +L+   ++   ++               V  +L  F    L  P      
Sbjct: 158 LLGALRALAVVLEHRPAALRLLL--------------LVAFTLVAFLFHVLLAPLTALFH 217

Query: 324 KKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSPHVDHY 383
                 L +    +PELYLYS  D VV    +E ++E R      V S DF SS HV H 
Sbjct: 218 THFYDRLLDAASRWPELYLYSRADEVVLARDVERMVEARLAHQVLVRSVDFVSSAHVSHL 272

Query: 384 RTYPDIYSSQLHKFL 398
           R YP  Y++    F+
Sbjct: 278 RDYPTYYTTLCINFM 272

BLAST of Lcy04g017650 vs. ExPASy Swiss-Prot
Match: Q6DJC8 (Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.0e-11
Identity = 64/261 (24.52%), Postives = 112/261 (42.91%), Query Frame = 0

Query: 144 VVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDL---AVE 203
           V+LLGW G K ++L +Y   Y+ +G   + +        W A+        S L   A +
Sbjct: 30  VILLGWGGCKDQYLAKYSAIYHNQGCTVIKYTA-----AWNAVFISESLGFSSLREDAKK 89

Query: 204 LISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLE-KIKGCIVDSGGGDP 263
           L+  L D E   +   ++FH FSN G+ +Y  I+E+L     L +  + G I DS  G  
Sbjct: 90  LLELLFDYE--IEKSPILFHVFSNGGFMLYRYIVELLHSHCRLNKLHVVGTIFDSAPG-- 149

Query: 264 LNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETFVLSSLEKFFSVALKL---P 323
            N  V  +  +   + + S++ +                 F+  +      + L++   P
Sbjct: 150 -NRNVIGSVRALDTILRTSTNNA---------------IRFLALAAFAIMVIILRIVLYP 209

Query: 324 EVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSS 383
                 +     + ++   +P+LYLYS  D ++ +  +E +I  R++      + DF  S
Sbjct: 210 VTKFLHENHYDAMKKDSSRWPQLYLYSRADPIISYIDVESMIAARRRCCLPTEALDFGKS 265

Query: 384 PHVDHYRTYPDIYSSQLHKFL 398
            HV H+R +P  YS     FL
Sbjct: 270 EHVSHFRRFPHRYSEMCTSFL 265

BLAST of Lcy04g017650 vs. ExPASy TrEMBL
Match: A0A5A7T4A9 (Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00160 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.6e-190
Identity = 340/403 (84.37%), Postives = 367/403 (91.07%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESNRSA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R KTGRK+ S++F  SPHVDHYRTYPDIYSSQLHKFL+ESFSS
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFLIESFSS 403

BLAST of Lcy04g017650 vs. ExPASy TrEMBL
Match: A0A1S3B1C0 (transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103485055 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.6e-190
Identity = 340/403 (84.37%), Postives = 367/403 (91.07%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESNRSA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R KTGRK+ S++F  SPHVDHYRTYPDIYSSQLHKFL+ESFSS
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFLIESFSS 403

BLAST of Lcy04g017650 vs. ExPASy TrEMBL
Match: A0A5D3CM53 (Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002010 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.1e-189
Identity = 339/403 (84.12%), Postives = 366/403 (90.82%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESNRSA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R KTGRK+ S++F  SPHVDHYRTYPDIYSSQLHKF +ESFSS
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFFIESFSS 403

BLAST of Lcy04g017650 vs. ExPASy TrEMBL
Match: A0A0A0KGH8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502770 PE=3 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 4.5e-188
Identity = 341/403 (84.62%), Postives = 364/403 (90.32%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR LFSKTIP SRLV+SPES RSA+FRQS TY H  PSR   GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGLFSKTIPQSRLVSSPESYRSAIFRQSSTYFHFSPSRKNLGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHC SS FGSS+SSLG  PSNFL SLPS+QSFGSQF  D+F+SFL DSNGS WTW
Sbjct: 61  LSLNLSHCCSSSFGSSASSLGSIPSNFLYSLPSLQSFGSQFASDYFSSFLFDSNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVGVL GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGVLRGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP+ SIELLI+K
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPPYPELYLYSSGDKVVPYTSIELLIKK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R  TGRKV S++F +SPHVDHYRTYPDIYS QLHKFL+ESFSS
Sbjct: 361 RMMTGRKVFSYNFGTSPHVDHYRTYPDIYSLQLHKFLIESFSS 403

BLAST of Lcy04g017650 vs. ExPASy TrEMBL
Match: A0A6J1IMV1 (transmembrane protein 53 OS=Cucurbita maxima OX=3661 GN=LOC111478463 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 6.4e-187
Identity = 335/403 (83.13%), Postives = 363/403 (90.07%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR LF+KTIP SRLV+ PE NR+A+FRQS TY H PP +   G K S
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPEFNRTAIFRQSSTYFHFPPRKCL-GLKFS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGS++SS+G N  NFLSSLPS+QSFGSQF PD+ NSFL DSNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSTASSIGSNAFNFLSSLPSLQSFGSQFAPDYSNSFLPDSNGSAWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRAS+SAI  NVG LGGEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTFV+DP E
Sbjct: 121 NRASDSAIGINVGALGGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLA ELISWLSDGE + KDR LIFHTFSNTGWFVYGA+LEI QGR
Sbjct: 181 FLWFALSRKVEQRISDLAAELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAVLEIFQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSSTSP+V G+E+DKKP  +ETF
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
           VLSSLE FFSVALK P+VDKRLKKIVS+LTE QP YPELYLYSSGD+VVPFESIELLIEK
Sbjct: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           RKKTG KVLS++F SSPHVDHYRTYPDIYSSQLHKFLV+SFSS
Sbjct: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVDSFSS 402

BLAST of Lcy04g017650 vs. NCBI nr
Match: XP_038883889.1 (transmembrane protein 53 [Benincasa hispida])

HSP 1 Score: 677.2 bits (1746), Expect = 8.9e-191
Identity = 344/403 (85.36%), Postives = 369/403 (91.56%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNRRLFSKTIP SRLV+SPES+RSA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRRLFSKTIPSSRLVSSPESDRSAIFRQSSTYFHLSPSRKYLGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG +PSNFLSSLPS+QSFGSQF  D+ NS LSDSNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSSPSNFLSSLPSLQSFGSQFASDYSNSVLSDSNGSAWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  ++GVLG EKG+ TVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDP E
Sbjct: 121 NRASESAIGNDLGVLGAEKGTVTVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLSDGE SD DR LIFH FSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSDGEESDNDRCLIFHAFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLL+KIKGCI DSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DK+P  +ET 
Sbjct: 241 KDLLKKIKGCIFDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEVDKRPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
           VLSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VV  ESIELLIEK
Sbjct: 301 VLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVLSESIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           RKKTGRKVLS++FR+SPHVDHYRTYPDIY+SQLHKFL+ESFSS
Sbjct: 361 RKKTGRKVLSYNFRTSPHVDHYRTYPDIYASQLHKFLIESFSS 403

BLAST of Lcy04g017650 vs. NCBI nr
Match: XP_008440723.1 (PREDICTED: transmembrane protein 53 [Cucumis melo] >KAA0036219.1 transmembrane protein 53 [Cucumis melo var. makuwa])

HSP 1 Score: 674.1 bits (1738), Expect = 7.5e-190
Identity = 340/403 (84.37%), Postives = 367/403 (91.07%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESNRSA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R KTGRK+ S++F  SPHVDHYRTYPDIYSSQLHKFL+ESFSS
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFLIESFSS 403

BLAST of Lcy04g017650 vs. NCBI nr
Match: TYK12615.1 (transmembrane protein 53 [Cucumis melo var. makuwa])

HSP 1 Score: 672.5 bits (1734), Expect = 2.2e-189
Identity = 339/403 (84.12%), Postives = 366/403 (90.82%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESNRSA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R KTGRK+ S++F  SPHVDHYRTYPDIYSSQLHKF +ESFSS
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFFIESFSS 403

BLAST of Lcy04g017650 vs. NCBI nr
Match: XP_011658015.1 (transmembrane protein 53 [Cucumis sativus])

HSP 1 Score: 667.2 bits (1720), Expect = 9.2e-188
Identity = 341/403 (84.62%), Postives = 364/403 (90.32%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR LFSKTIP SRLV+SPES RSA+FRQS TY H  PSR   GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGLFSKTIPQSRLVSSPESYRSAIFRQSSTYFHFSPSRKNLGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHC SS FGSS+SSLG  PSNFL SLPS+QSFGSQF  D+F+SFL DSNGS WTW
Sbjct: 61  LSLNLSHCCSSSFGSSASSLGSIPSNFLYSLPSLQSFGSQFASDYFSSFLFDSNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVGVL GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGVLRGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+GE SDKDRSLIFHTFSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLP+VDKRL  IVSVLTENQP YPELYLYSSGD+VVP+ SIELLI+K
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPPYPELYLYSSGDKVVPYTSIELLIKK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           R  TGRKV S++F +SPHVDHYRTYPDIYS QLHKFL+ESFSS
Sbjct: 361 RMMTGRKVFSYNFGTSPHVDHYRTYPDIYSLQLHKFLIESFSS 403

BLAST of Lcy04g017650 vs. NCBI nr
Match: XP_023543057.1 (transmembrane protein 53 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.2 bits (1715), Expect = 3.5e-187
Identity = 337/403 (83.62%), Postives = 363/403 (90.07%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNRSALFRQSPTYLHPPPSRTFPGSKIS 60
           MEAPVRAFRP VLNR LF+KTIP SRLV+ PESNR+A+ RQS TY H PP +   G K S
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCL-GLKFS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGS++SS+G +  NFLSSLPS+QS GSQF PD+ NSFLSDSNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG LGGEKG ATVVLLGWLG+KTKHLRRYVEWYNARGINALTFV+DP E
Sbjct: 121 NRASESAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLA ELISWLSDGE + KDR LIFHTFSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEELDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSSTSP+V G+E+DKKP  +ETF
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300

Query: 301 VLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEK 360
           VLSSLE FFSVALK P+VDKRLKKIVS+LTE QP YPELYLYSSGD+VVPFESIELLIEK
Sbjct: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFSS 404
           RKKTG KVLS++F SSPHVDHYRTYPDIYSSQLHKFLVESF+S
Sbjct: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402

BLAST of Lcy04g017650 vs. TAIR 10
Match: AT2G18245.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 5.0e-91
Identity = 175/335 (52.24%), Postives = 228/335 (68.06%), Query Frame = 0

Query: 78  SSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTWNRASESAI-KGNVGVLG 137
           SSL  N + F S      +F  +    H N F S+ N     WNRA       GN  + G
Sbjct: 57  SSLPANANLFRSLFFLTSTFRLENTAVHANPFRSEPNDGVAVWNRAPRIVNGGGNAAIFG 116

Query: 138 GEKG-SATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRIS 197
           G +G   TVVLLGWLG+K KHLRRYVEWYN+RGINA+TF VD  + L   L RR+E+RI+
Sbjct: 117 GNEGKETTVVLLGWLGAKAKHLRRYVEWYNSRGINAVTFTVDVRDLLRLDLGRRLERRIA 176

Query: 198 DLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEKIKGCIVDSG 257
           +   EL++W+S+ E   +++ L+FH+FSNTGW VYGA+LE   GR+DL+E+IKGCI+DSG
Sbjct: 177 EFGNELVNWVSEKEDDGREKCLVFHSFSNTGWLVYGALLESFIGRQDLVERIKGCIIDSG 236

Query: 258 GGDPLNPQVWAAGFSAAILKKNSS--STSPVVDGEELD------KKPPWMETFVLSSLEK 317
           G DPL+ ++WAAGF+AAILKK SS  +T P    +E D      K+P  +E  +LSSLEK
Sbjct: 237 GADPLDTKIWAAGFTAAILKKRSSTITTEPNSPIKEEDASTPQKKEPLGIENIMLSSLEK 296

Query: 318 FFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGDRVVPFESIELLIEKRKKTGRK 377
            F + L  P+V+ RL KI+  L EN P  P+LYLYSSGD+VVP  S+EL I +++K GRK
Sbjct: 297 LFPIFLNHPDVNTRLTKIIQKLYENHPPCPQLYLYSSGDKVVPSHSVELRIREQQKIGRK 356

Query: 378 VLSHDFRSSPHVDHYRTYPDIYSSQLHKFLVESFS 403
           + S +F+SSPHVDHYR +PD+YSSQL  FL E F+
Sbjct: 357 IHSFNFKSSPHVDHYRNFPDLYSSQLQNFLQECFT 391

BLAST of Lcy04g017650 vs. TAIR 10
Match: AT3G19970.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.9e-62
Identity = 122/291 (41.92%), Postives = 186/291 (63.92%), Query Frame = 0

Query: 117 TWTWNRASESAI--KGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTF 176
           ++ W+    SA+   G+   +  EK    VVLLGWLGSK KHL++Y +WY ++G + +TF
Sbjct: 137 SYRWHLPEPSAVDMSGSSSCV-SEKNRTVVVLLGWLGSKQKHLKKYADWYTSKGYHVITF 196

Query: 177 VVDPTEFLWFALSRRVEQRISDLAVELISWLSDGEGSDKDRSLIFHTFSNTGWFVYGAIL 236
            +   E + + +  + E+ I  L   L  WL +    ++ ++L+FHTFSNTGW  YGAIL
Sbjct: 197 TLPMNEIMSYQVGGKAEKNIESLVNHLADWLDE----EQKKNLVFHTFSNTGWLTYGAIL 256

Query: 237 EILQGR-KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKN------SSSTSPVVDG 296
           E  Q +   L+ ++KGCIVDS      +P VWA+GFSAA LKK+      S+S+S   +G
Sbjct: 257 EKFQKQDSSLMGRVKGCIVDSAPVAAADPTVWASGFSAAFLKKSSVATKGSASSSYESNG 316

Query: 297 EELDK-KPPWMETFVLSSLEKFFSVALKLPEVDKRLKKIVSVLTENQPLYPELYLYSSGD 356
             + + KP   ET +L  LEKFF+V L LP+V++RL  ++  L+  QP  P+LY+YSS D
Sbjct: 317 INISQPKPAATETALLLVLEKFFAVILNLPKVNRRLADVLDTLSTAQPSCPQLYIYSSAD 376

Query: 357 RVVPFESIELLIEKRKKTGRKVLSHDFRSSPHVDHYRTYPDIYSSQLHKFL 398
           RV+P E +E  I +++K G +V + +F SSPHVDH+R+ P++Y+++L+ F+
Sbjct: 377 RVIPVEQVESFIVEQRKAGHEVRACNFISSPHVDHFRSNPELYTAELNHFM 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6P2H88.5e-1932.06Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1[more]
Q9D0Z32.1e-1729.62Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1[more]
Q6DHN04.7e-1727.59Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1[more]
Q2TBP58.0e-1729.41Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1[more]
Q6DJC81.0e-1124.52Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T4A93.6e-19084.37Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3B1C03.6e-19084.37transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103485055 PE=3 SV=1[more]
A0A5D3CM531.1e-18984.12Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0KGH84.5e-18884.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502770 PE=3 SV=1[more]
A0A6J1IMV16.4e-18783.13transmembrane protein 53 OS=Cucurbita maxima OX=3661 GN=LOC111478463 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883889.18.9e-19185.36transmembrane protein 53 [Benincasa hispida][more]
XP_008440723.17.5e-19084.37PREDICTED: transmembrane protein 53 [Cucumis melo] >KAA0036219.1 transmembrane p... [more]
TYK12615.12.2e-18984.12transmembrane protein 53 [Cucumis melo var. makuwa][more]
XP_011658015.19.2e-18884.62transmembrane protein 53 [Cucumis sativus][more]
XP_023543057.13.5e-18783.62transmembrane protein 53 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G18245.15.0e-9152.24alpha/beta-Hydrolases superfamily protein [more]
AT3G19970.14.9e-6241.92alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008547Protein of unknown function DUF829, TMEM53PFAMPF05705DUF829coord: 143..397
e-value: 2.1E-59
score: 201.3
IPR008547Protein of unknown function DUF829, TMEM53PANTHERPTHR12265UNCHARACTERIZEDcoord: 81..401
NoneNo IPR availablePANTHERPTHR12265:SF11ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 81..401
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 138..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g017650.1Lcy04g017650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0097573 glutathione oxidoreductase activity