Lcy04g003210 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g003210
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr04: 24178027 .. 24179653 (+)
RNA-Seq ExpressionLcy04g003210
SyntenyLcy04g003210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTTTAGAGACCTAGCTATGTTTAACAAGGCCATGTTAGCAAAGCTAAGTTGGAGAATCATTAAATCCCCTGATAGCCTTATGGCTAAAGTCCTCAAAGGCAAATATTTCAGCGGAGGGGCTTTCCTAAAGGCCAAAGAGGGCTGCAAAGCTTTTATCGTGTGGAAAAACATATTATGGGGCAGATCTCTATTTGAAAAAGGCTACATATGGAGGATTGGTAACGACAAACAAGTATATATCGACCAAGATCCATGGTTGATAGACAAAGGGGGCTGGATTCCTAAAGGAGTTAAGGAGGTACTTCAAGGAAAAAGAGTGGCCGACATTTTAGATGATAGTGGAGTCTGGAAGGAAGATCTCATAAGAGACGCCTTTCTCTCAAGTGATGTTGACTCCATTCTGAGTATGCCAAGGAATAGAATCCCTAAAGATGACGAAGTAATCTGGGGAACGGACCCTAAAGGAATCTTTACAATAAAAGGGGCTTATCATCTTGCTATCAATAATGAAAATCAATCATTAGCCTCCGAATCGAACCCTTCCCCATCCAAAAGGTTGTGGAATTTTATATGGAATCTGGATGTCTGGCCTAGAATTAAAATCACAATTTGGAAAGCTCTCAACAACGCACTCCCCACTCTTGAGAATATTAGAAAACTAGGGGTTAATACTAATCCGATGTGTTGGTTTTGCAGGTCGAAGCCAGAGGACTCGGAGCATCTTTTCTGGAATTGCAAAGTTGCTAAAGGTATTTGGGTGAATTTGATTCCTAACCTAATGCAAATTTTTTCTTGCAGCGGGGGAGGATGGAGCTCGGTGGACTATTGGGATCATGCTACTGAGATTCTTGATAATTCAAAAATCACTAGAGCTGGCCAAATTATATGGTCCATCTGGAACACTAAGAATCAGGTGAAGCAAATAAATCACAAGGCAGACCCATATCAATGCATCACAGAAATCAAGCAGAGAATGACTTTTTTCGAGAATAGGGGGGAAAAATTACCAAGCAGCTAGCAAGTCGGAGACCCACCCTAGTCACGACAAATGGAAGCCGCCAGATGCCACCCATTGGAAGCTCGATGCAGATGCGGTGTGGTTCGATGATTCACATTCCGACGGCGTTGGGTGGGCCATTCGTGACTCAGAAGGTTCTCTGATAGGAGCGGGCTGCAAGAAGATCAGGAATAAGGAAGCCATAAAGATGCTCGAAGCTAAAGCCATTAGAGAAGGGTTGGAAAAGCTTACCGATCGTGTCAGGCTCGGTGGGCTTAGGAGTAATCAAGCGCTGGAGGTCGAGTCGGATTCGCCTGAAATCGTGAACCTCCTTAACGAAAAGGCAGAGGACCTGCCGGAGACGACCTTTGTGGTCGAAGAGGTGCTGGAGCTGGTCGGAAAGATGAACGTCATCTCCTTCTCCTCGTGCCCAAGAGTGGGCAATTTTTTGGAGTACTGTCTTGCGCGCGCAACTGCACATTTTGGGAATTTAGTTTTTTTTGTCGATACACCATCTCAGTGCTTGAGAAGGGATATTGCAGGGGGCTTATTTGATGCCGCTGATTGGTTTGCCTCGGTTTTAAGAGGCTGTGATGGAATTTCTGACTTTAATCCGTCTATTTAA

mRNA sequence

ATGAGCTTTAGAGACCTAGCTATGTTTAACAAGGCCATGTTAGCAAAGCTAAGTTGGAGAATCATTAAATCCCCTGATAGCCTTATGGCTAAAGTCCTCAAAGGCAAATATTTCAGCGGAGGGGCTTTCCTAAAGGCCAAAGAGGGCTGCAAAGCTTTTATCGTGTGGAAAAACATATTATGGGGCAGATCTCTATTTGAAAAAGGCTACATATGGAGGATTGGTAACGACAAACAAGTATATATCGACCAAGATCCATGGTTGATAGACAAAGGGGGCTGGATTCCTAAAGGAGTTAAGGAGGTACTTCAAGGAAAAAGAGTGGCCGACATTTTAGATGATAGTGGAGTCTGGAAGGAAGATCTCATAAGAGACGCCTTTCTCTCAAGTGATGTTGACTCCATTCTGAGTATGCCAAGGAATAGAATCCCTAAAGATGACGAAGTAATCTGGGGAACGGACCCTAAAGGAATCTTTACAATAAAAGGGGCTTATCATCTTGCTATCAATAATGAAAATCAATCATTAGCCTCCGAATCGAACCCTTCCCCATCCAAAAGGTTGTGGAATTTTATATGGAATCTGGATGTCTGGCCTAGAATTAAAATCACAATTTGGAAAGCTCTCAACAACGCACTCCCCACTCTTGAGAATATTAGAAAACTAGGGGTTAATACTAATCCGATGTGTTGGTTTTGCAGGTCGAAGCCAGAGGACTCGGAGCATCTTTTCTGGAATTGCAAAGTTGCTAAAGCGGGGGAGGATGGAGCTCGGTGGACTATTGGGATCATGCTACTGAGATTCTTGATAATTCAAAAATCACTAGAGCTGGCCAAATTATATGGTCCATCTGGAACACTAAGAATCAGAATAGGGGGGAAAAATTACCAAGCAGCTAGCAAGTCGGAGACCCACCCTAGTCACGACAAATGGAAGCCGCCAGATGCCACCCATTGGAAGCTCGATGCAGATGCGGTGTGGTTCGATGATTCACATTCCGACGGCGTTGGGTGGGCCATTCGTGACTCAGAAGGTTCTCTGATAGGAGCGGGCTGCAAGAAGATCAGGAATAAGGAAGCCATAAAGATGCTCGAAGCTAAAGCCATTAGAGAAGGGTTGGAAAAGCTTACCGATCGTGTCAGGCTCGGTGGGCTTAGGAGTAATCAAGCGCTGGAGGTCGAGTCGGATTCGCCTGAAATCGTGAACCTCCTTAACGAAAAGGCAGAGGACCTGCCGGAGACGACCTTTGTGGTCGAAGAGGTGCTGGAGCTGGTCGGAAAGATGAACGTCATCTCCTTCTCCTCGTGCCCAAGAGTGGGCAATTTTTTGGAGTACTGTCTTGCGCGCGCAACTGCACATTTTGGGAATTTAGTTTTTTTTGTCGATACACCATCTCAGTGCTTGAGAAGGGATATTGCAGGGGGCTTATTTGATGCCGCTGATTGGTTTGCCTCGGTTTTAAGAGGCTGTGATGGAATTTCTGACTTTAATCCGTCTATTTAA

Coding sequence (CDS)

ATGAGCTTTAGAGACCTAGCTATGTTTAACAAGGCCATGTTAGCAAAGCTAAGTTGGAGAATCATTAAATCCCCTGATAGCCTTATGGCTAAAGTCCTCAAAGGCAAATATTTCAGCGGAGGGGCTTTCCTAAAGGCCAAAGAGGGCTGCAAAGCTTTTATCGTGTGGAAAAACATATTATGGGGCAGATCTCTATTTGAAAAAGGCTACATATGGAGGATTGGTAACGACAAACAAGTATATATCGACCAAGATCCATGGTTGATAGACAAAGGGGGCTGGATTCCTAAAGGAGTTAAGGAGGTACTTCAAGGAAAAAGAGTGGCCGACATTTTAGATGATAGTGGAGTCTGGAAGGAAGATCTCATAAGAGACGCCTTTCTCTCAAGTGATGTTGACTCCATTCTGAGTATGCCAAGGAATAGAATCCCTAAAGATGACGAAGTAATCTGGGGAACGGACCCTAAAGGAATCTTTACAATAAAAGGGGCTTATCATCTTGCTATCAATAATGAAAATCAATCATTAGCCTCCGAATCGAACCCTTCCCCATCCAAAAGGTTGTGGAATTTTATATGGAATCTGGATGTCTGGCCTAGAATTAAAATCACAATTTGGAAAGCTCTCAACAACGCACTCCCCACTCTTGAGAATATTAGAAAACTAGGGGTTAATACTAATCCGATGTGTTGGTTTTGCAGGTCGAAGCCAGAGGACTCGGAGCATCTTTTCTGGAATTGCAAAGTTGCTAAAGCGGGGGAGGATGGAGCTCGGTGGACTATTGGGATCATGCTACTGAGATTCTTGATAATTCAAAAATCACTAGAGCTGGCCAAATTATATGGTCCATCTGGAACACTAAGAATCAGAATAGGGGGGAAAAATTACCAAGCAGCTAGCAAGTCGGAGACCCACCCTAGTCACGACAAATGGAAGCCGCCAGATGCCACCCATTGGAAGCTCGATGCAGATGCGGTGTGGTTCGATGATTCACATTCCGACGGCGTTGGGTGGGCCATTCGTGACTCAGAAGGTTCTCTGATAGGAGCGGGCTGCAAGAAGATCAGGAATAAGGAAGCCATAAAGATGCTCGAAGCTAAAGCCATTAGAGAAGGGTTGGAAAAGCTTACCGATCGTGTCAGGCTCGGTGGGCTTAGGAGTAATCAAGCGCTGGAGGTCGAGTCGGATTCGCCTGAAATCGTGAACCTCCTTAACGAAAAGGCAGAGGACCTGCCGGAGACGACCTTTGTGGTCGAAGAGGTGCTGGAGCTGGTCGGAAAGATGAACGTCATCTCCTTCTCCTCGTGCCCAAGAGTGGGCAATTTTTTGGAGTACTGTCTTGCGCGCGCAACTGCACATTTTGGGAATTTAGTTTTTTTTGTCGATACACCATCTCAGTGCTTGAGAAGGGATATTGCAGGGGGCTTATTTGATGCCGCTGATTGGTTTGCCTCGGTTTTAAGAGGCTGTGATGGAATTTCTGACTTTAATCCGTCTATTTAA

Protein sequence

MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNILWGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILDDSGVWKEDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLASESNPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPEDSEHLFWNCKVAKAGEDGARWTIGIMLLRFLIIQKSLELAKLYGPSGTLRIRIGGKNYQAASKSETHPSHDKWKPPDATHWKLDADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRLGGLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCPRVGNFLEYCLARATAHFGNLVFFVDTPSQCLRRDIAGGLFDAADWFASVLRGCDGISDFNPSI
Homology
BLAST of Lcy04g003210 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.2e-11
Identity = 31/91 (34.07%), Postives = 56/91 (61.54%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           + FRDL  FN+A+LAK S+RII  P +L++++L+ +YF   + ++   G +    W++I+
Sbjct: 57  LGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSII 116

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDK 92
            GR L  +G +  IG+     +  D W++D+
Sbjct: 117 HGRELLSRGLLRTIGDGIHTKVWLDRWIMDE 147

BLAST of Lcy04g003210 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.3e-55
Identity = 136/439 (30.98%), Postives = 223/439 (50.80%), Query Frame = 0

Query: 1    MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
            M FRDL +FNKA+LAK  WRI+  P+S++++VLKG+YF   +F++AK       +W++IL
Sbjct: 646  MGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSIL 705

Query: 61   WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILD-DSGVWK 120
            WGR L +KG  WRIGN   V+I  D W+ ++          +    RV+ ++D + G W+
Sbjct: 706  WGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQ 765

Query: 121  EDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLA-INNENQSLAS 180
             D++RD F   +   ILS+P  R  ++D +IW  +  G+++++  Y +A +NN      S
Sbjct: 766  GDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPS 825

Query: 181  ESNPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPE 240
             S+    +  WN  W + +  +IK+ +W+   + LPT  N+ K GV     C+FC    E
Sbjct: 826  SSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGE 885

Query: 241  DSEHLFWNCKVAKAGEDGARWTIGIMLLRFLIIQKSLEL--------------------- 300
            DS HLFW CK A+A    +++     L  FLI+++S E                      
Sbjct: 886  DSIHLFWICKFAEALWINSKFG---KLSPFLILRESHESLSKADFEELCVVIWGLWNQRN 945

Query: 301  AKLYGPSGTLRIRIGGKNYQAASK---------------SETHPSHDKWKPPDATHWKLD 360
            A+ +  S     +IG +  + A+K                 T+ +   W+PPD   +K++
Sbjct: 946  ARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAEILWQPPDEGIYKIN 1005

Query: 361  ADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRL 402
             DA +       G+G  I +  G ++ A  K + N +++ M EA A  EGL+  ++    
Sbjct: 1006 TDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASE---- 1065

BLAST of Lcy04g003210 vs. ExPASy TrEMBL
Match: A0A1S8AC01 (Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.4e-53
Identity = 144/479 (30.06%), Postives = 230/479 (48.02%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           + F+D + FN+A++AK  WR+++ PDSL A+VL+ KYF    FL AK G     +W++I+
Sbjct: 49  LGFKDFSSFNQALVAKQGWRLLQHPDSLAARVLEAKYFKKADFLNAKLGSNPSFIWRSII 108

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILDDSGVWKE 120
           WGR +   G  WRIG   +V I +  W+     + P     + +   V+ ++DD   W E
Sbjct: 109 WGRQVILAGSRWRIGKGDKVQIYKASWIPRPSTFRPTSRPTLPENSYVSMLIDDDNQWDE 168

Query: 121 DLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLASES 180
             I   F   D D I+SMP  R PKDD+++W  D KG +++K  Y +A+  +  +L S S
Sbjct: 169 MKIYCHFDKLDADRIVSMPLPRQPKDDQILWHYDKKGRYSVKSGYQVALRLKFPALPSSS 228

Query: 181 NPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPEDS 240
             + ++  W  IW LD+  +++I +W+A  N LP+ EN+ K  V   P+C  C++  E  
Sbjct: 229 RNTTNQ--WETIWALDLPEKLRIFMWRAATNLLPSTENLWKRKVAQEPICQICKNGVESI 288

Query: 241 EHLFWNCKVAK---------------AGEDGARWTIGIMLLRFLIIQKSLELA-----KL 300
            H   NCK A+               AGED      G +  R    +  +EL       +
Sbjct: 289 FHALVNCKTARKVWRLTQFAEELQEEAGEDLLSLLKGRLCSR---RKADIELLVAICWGI 348

Query: 301 YGPSGTLRIRIGGKNYQ-AASKSE--------TH---PSH---------DKWKPPDATHW 360
           +        ++  ++ Q   +K+E        TH   P H           W PP A  +
Sbjct: 349 WSARNQFIFKLKKEDPQIVLAKAEAILKAYKRTHVPAPIHMDQQAWKAQQDWSPPLAGWF 408

Query: 361 KLDADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDR 420
           KL+ DA    D  + G+G  IR++EG ++ A     +    +  +EA+A+  GL+     
Sbjct: 409 KLNVDAATNRDKQTSGLGVVIRNAEGIVVAAAINCSKFCGDVAYVEAEAMEFGLQ----- 468

Query: 421 VRLGGLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCPR 439
             + G     +L VE+DS E+V+ +N K   + E  +VV  +  L+   N I     PR
Sbjct: 469 --VAGNAKLPSLIVETDSQEVVDFVNNKQSSMKEIFWVVAAIQNLMRNFNQIKVQHIPR 515

BLAST of Lcy04g003210 vs. ExPASy TrEMBL
Match: A0A1S8ACU2 (Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.2e-53
Identity = 143/494 (28.95%), Postives = 234/494 (47.37%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           + FRD+  FN+A++AK SWRIIK P+SLMAK+L+ KYF G  FL+AK G K   VW++I+
Sbjct: 98  LGFRDITSFNQALVAKQSWRIIKYPESLMAKILQAKYFKGADFLQAKLGSKPSFVWRSII 157

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILDDSGVWKE 120
           WGR +   G  WRIG   +V I +  W+     + P     +     VA+++D++  WKE
Sbjct: 158 WGRQVIVNGMRWRIGTGDRVKIYKSHWIPRPQAFKPISAPTLGMDCTVAELIDENQKWKE 217

Query: 121 DLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLASES 180
            LI+  F   D + I  +     PK D+++W  D KG +++K  Y +A+  ++ +  S S
Sbjct: 218 SLIQQHFNFEDAELISRIQLPISPKPDQILWHYDKKGNYSVKSGYQIAMRIKHPARPSSS 277

Query: 181 NPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPEDS 240
           + +  +  WN IW+L++  +IKI +WKA  N LPT EN+ +  +   P+C  C+ K ED 
Sbjct: 278 SSNLGQ--WNVIWSLELPEKIKIFMWKAARNFLPTSENLWRRKMVQEPICPRCKMKKEDI 337

Query: 241 EHLFWNCKVAKAGEDGARWTIG--------------IMLLRFLIIQKS-----LELAKLY 300
            H    CK AK       W +               + +L+ L+ ++S     L +A  +
Sbjct: 338 NHAIMVCKAAK-----KMWKLTPFVEEMQLLDNQDLLSMLQELVNRRSKDELRLIIALCW 397

Query: 301 GPSGTLRI-------------------------RIGGKNYQAASKSETHPSHDKWKPPDA 360
               T  I                         R+     QAA+K+       KW PP  
Sbjct: 398 TAWHTRNIFVFENKRQDPQISVAKAEAVVESYARVRMTKVQAAAKA-IPAKGAKWIPPPQ 457

Query: 361 THWKLDADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKL 420
            H+K + DA    + +  G+G  IRD  G++I A     +    +   EA A+  GL+ +
Sbjct: 458 GHFKANVDAAVNKEKNQVGLGVVIRDDSGAIIIAAVNHTKYHGDVAQAEAAAVNFGLQVV 517

Query: 421 TDRVRLGGLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMN-VISFSS 450
            +        S   L +E+D  ++V+ +        E  + + E+ + +   N ++    
Sbjct: 518 ME-------ASLSPLILETDCQDVVDFVLHNKSSRTEIYWTISEIQQKLQSFNSMVELQK 576

BLAST of Lcy04g003210 vs. ExPASy TrEMBL
Match: A0A2N9FFZ2 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13985 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.3e-51
Identity = 136/466 (29.18%), Postives = 227/466 (48.71%), Query Frame = 0

Query: 1    MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
            M  RDL  FN+A+LAK  WR++ +P SL +KV K KYF   + L+A++  +    WK+I+
Sbjct: 1167 MGLRDLGFFNEALLAKQVWRLLHNPSSLFSKVFKAKYFPHCSILEAQQRSQGSYAWKSIM 1226

Query: 61   WGRSLFEKGYIWRIGNDKQVYIDQDPWLID-KGGWIPKGVKEVLQGKRVADILD-DSGVW 120
              R L  KG +WR+G    + I +D WL D     I       +    V  ++D +   W
Sbjct: 1227 SARDLIVKGSVWRVGTGSHIRIWRDKWLPDTHSHCIVSPPPPSISISHVQHLIDSELRSW 1286

Query: 121  KEDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLAS 180
            K +L++  FL  +   IL +P +     D ++W     G +T++  YHL +N  +Q   S
Sbjct: 1287 KAELVKRIFLPHEASVILGIPLSIRDPIDSLVWKATKNGAYTVRSGYHLLLNECHQDEPS 1346

Query: 181  ESNPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPE 240
             S+ +   +LW+ IW+L V P+I+  +W+A +N+LPT  N+    +  +P C  C ++ E
Sbjct: 1347 SSDTTKMTQLWDAIWSLHVPPKIRHFLWRACHNSLPTRSNLHHRHILADPSCSSCTNQIE 1406

Query: 241  DSEHLFWNCKVAKAGEDGARWTIGIMLLRF-----LIIQKSLELAKLYGPSGTLRIRIGG 300
             + H  W CK  K       W   +  + +     LI+Q+ ++      P     +    
Sbjct: 1407 STIHALWQCKEIKPVWQSIPWGRKLREISYAGFIDLILQQPVDNLSQLIPRALDTL---- 1466

Query: 301  KNYQAASKSETHPS----HDK---WKPPDATHWKLDADAVWFDDSHSDGVGWAIRDSEGS 360
              +Q A  S+  PS    H K   WKPP+   +K++ D   F + +  GVG  IR+  G 
Sbjct: 1467 LEFQTAQNSDPQPSPKPNHTKSTTWKPPEEGRYKVNYDGAVFSERNEAGVGVIIRNYRGE 1526

Query: 361  LIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRLGGLRSNQALEVESDSPEIVNLLNE 420
            ++G+   +I    +++ +EA A    ++   D   LG +     +++E DS  IV  L  
Sbjct: 1527 VMGSLSHRIPYPHSVEAVEASAASCAIQFAKD---LGFM----LVDLEGDSKIIVEALLL 1586

Query: 421  KAEDLPETTF---VVEEVLELVGKMNVISFSSCPRVGNFLEYCLAR 450
            KA   P TT    V+E++ +    +  + F    R GN + + LA+
Sbjct: 1587 KA---PCTTIYGNVIEDIKQSAQNLQSVHFLHINREGNAMAHLLAK 1618

BLAST of Lcy04g003210 vs. ExPASy TrEMBL
Match: A0A5B6WTC7 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_007180 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.9e-51
Identity = 141/485 (29.07%), Postives = 226/485 (46.60%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           M FRDL+ FNKA+LAK +WR++  PD L+AKV K +YF    FL A  G      W++I 
Sbjct: 241 MGFRDLSFFNKALLAKQAWRLLTQPDCLLAKVYKARYFPFTNFLSASVGSYPSFTWRSIC 300

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLI--DKGGWIPKGVKEVLQGKRVADILDDSG-V 120
             R L + G IWRIGN K V I  DPWL+  D    + + +   +Q   V  ++DDS   
Sbjct: 301 GARELIDNGLIWRIGNGKMVNIWNDPWLLGNDNNRLLVQNMN--IQWTTVNHLIDDSSFT 360

Query: 121 WKEDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLA 180
           WKED+I     S   + ILS+P  R   +D ++W  D KGI+T+K  Y + I + +Q+++
Sbjct: 361 WKEDVILKIAESDQANRILSIPLARPDAEDILVWRYDNKGIYTVKSGYRVLITSRSQTIS 420

Query: 181 SESNPSPS-KRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSK 240
           +  + +   K  +  +W L +  +IK+ +W+ + N +P L N+ K  + T  +C  C++ 
Sbjct: 421 NTMDDTGKYKSFYKQLWELQLPSKIKVHMWRLIKNYIPHLCNLHKRKLTTEVVCPLCKAG 480

Query: 241 PEDSEHLFWNC----------------------------KVAKAGEDGARWTIGIMLLRF 300
           PED+ HL W+C                             +  A  D  R  + I L   
Sbjct: 481 PEDANHLMWSCDFVRRLWKLLDLYAGLGNSTSEGKDQLVSIFTAMTDEKRKLLSISLWAI 540

Query: 301 LIIQ-KSLELAKLYGPSGTLR-IRIGGKNYQAASKSETHPSHDK---WKPPDATHWKLDA 360
             ++ K +   + +    T+R I+      Q + KS+  P   K   W+PP     KL+ 
Sbjct: 541 WFMRNKLIHEGRKFSMQETVRFIQRYVHELQMSCKSDFTPDTKKAIFWRPPPVDFVKLNF 600

Query: 361 DAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRLG 420
           DA +  +S S       R+ EG ++GA   +        + EA+A    +    D     
Sbjct: 601 DASFDKESKSAITAVVARNDEGLIMGACVYQCTEVADAFVAEARACERAILFAMD----- 660

Query: 421 GLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCPRVGNFL 449
                Q + +E DS  ++  L     D      +++ + ++   +  +SFS  PR GN  
Sbjct: 661 --MGFQKIILEGDSLTVIKKLRSAKNDRSVIRSIIQNICKIATLVEEVSFSFSPRKGNQA 716

BLAST of Lcy04g003210 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 246.5 bits (628), Expect = 4.8e-61
Identity = 149/492 (30.28%), Postives = 233/492 (47.36%), Query Frame = 0

Query: 1    MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
            + FRDL  FN+A++AK  WR+++ P+SLMA+V+K +Y+    F  AK G     +W++IL
Sbjct: 947  LGFRDLPSFNQALVAKQGWRLVRYPNSLMARVMKARYYKNSTFWNAKVGSNPSFIWRSIL 1006

Query: 61   WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILDDSGVWKE 120
            WG  + +KG  WRIG+ K+V + +D W+     + P   K +     VAD++D    W+ 
Sbjct: 1007 WGSQVIKKGVRWRIGDGKKVLVYKDKWIPRPATFQPISPKTLPHETVVADLIDSENKWRV 1066

Query: 121  DLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLASES 180
            D +   F+  D+++IL +      ++DEV+W  D KG +++K  Y LA+N    +    S
Sbjct: 1067 DRLEQHFMKEDIEAILKILLPSGKEEDEVLWHFDKKGEYSVKSGYQLALNQNFPNEPESS 1126

Query: 181  NPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPEDS 240
            N   S RLW   W LD+  ++KI +W+AL N LPT EN+ K      P+C  C+ + E  
Sbjct: 1127 N--SSSRLWKIPWMLDLPEKVKIFMWRALKNILPTAENLWKRRSLQEPICQRCKLQVETV 1186

Query: 241  EHLFWNCKVAKAGEDGAR------------------------------------WTIGIM 300
             H+   CK A+   D A                                     W I   
Sbjct: 1187 SHVLIECKAARKIWDLAPLIVQPSKDHNQDFFSAIQEMWSRSSTAEAELMIVYCWVIWSA 1246

Query: 301  LLRFLIIQKSLELAKLYGPSGTLRIRIGGKNYQAASK-SETHPSHD------KWKPPDAT 360
              +F+   K  +   L   + ++      K YQ  SK    H + D      KWKPP   
Sbjct: 1247 RNKFIFEGKKSDSRFLAAKADSVL-----KAYQRVSKPGNVHGAKDRGIDQQKWKPPSQN 1306

Query: 361  HWKLDADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLT 420
              KL+ DA         G+G  +RD+EG ++  G K+ + +E + + EA+AI  GL+   
Sbjct: 1307 VLKLNVDAAVSTKDQKVGLGAIVRDAEGKILAVGIKQAQFRERVSLAEAEAIHWGLQ--- 1366

Query: 421  DRVRLGGLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCP 450
                +    S+ +L VESD  E+V LLN       E  +++ +V     +   + FS  P
Sbjct: 1367 ----VANQISSSSLIVESDCKEVVELLNNTKGSRTEIHWILSDVRRESKEFKQVQFSFIP 1424

BLAST of Lcy04g003210 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 228.0 bits (580), Expect = 1.8e-55
Identity = 145/504 (28.77%), Postives = 239/504 (47.42%), Query Frame = 0

Query: 1    MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
            M FRDL+ FN+A++AK  WRI++ P SL+A+VLK +YF    F+ A  G K   VW++I+
Sbjct: 652  MGFRDLSSFNQALVAKQGWRIMQFPSSLVARVLKARYFKHTGFMNAGLGSKPSFVWRSIV 711

Query: 61   WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILDDSGVWKE 120
            WGR +  KG  WRIGN + V +  + W+     + P     +     VA+++D+   W+E
Sbjct: 712  WGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMGTDTTVAELIDEKQQWRE 771

Query: 121  DLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLASES 180
            DLI   F   D ++I+ +P  + PK+D++IW  D KG +++K  Y +A+  +     S S
Sbjct: 772  DLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKSGYQVAMRIKFPEDPSCS 831

Query: 181  NPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPEDS 240
            N    + LW FIW L +  ++KI +W+A ++ LPT EN+ K  V   PMC  C    E  
Sbjct: 832  N--HDQNLWRFIWKLAIPEKVKIFLWRAAHDLLPTAENLWKKKVLQEPMCQSCHCHVETV 891

Query: 241  EHLFWNCKVAK-------------------------------AGEDGAR-----WTIGIM 300
             H    C  A+                               A  +GA      W I   
Sbjct: 892  SHALVECNRARKIWRYSNLAEELRGVYRCDIVWMLQFWPRQHAKVEGAEVAALLWAIWKA 951

Query: 301  LLRFLIIQKSLELAKLYGPSGTL-----RIRIGGKNYQAASKSETHPSHDKWKPPDATHW 360
              ++L   K     ++   +  +     +IR     Y+    +E      +W PP     
Sbjct: 952  RNKWLFEGKKENPLRVVANAEAIVESFKKIRQPEMVYKTKGNAE---RQKQWSPPPNGWQ 1011

Query: 361  KLDADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDR 420
            K++ DA    ++   G+G  +RDS+G+   A  K +R   ++ M EA A+  GL K+ ++
Sbjct: 1012 KVNVDAAVDVENQMAGLGVVVRDSDGNCRAAAIKSLRLPGSVAMAEATAMEWGL-KVAEK 1071

Query: 421  VRLG-GLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCPR 463
              +  G+        ESDS E+++L+N+K+  L E  +++ ++ E +           PR
Sbjct: 1072 AHITFGI-------FESDSLEVIDLINKKSSSLTEIGWLISDIQENLQNFQNFKAQHSPR 1131

BLAST of Lcy04g003210 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 224.9 bits (572), Expect = 1.5e-54
Identity = 136/439 (30.98%), Postives = 223/439 (50.80%), Query Frame = 0

Query: 1    MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
            M FRDL +FNKA+LAK  WRI+  P+S++++VLKG+YF   +F++AK       +W++IL
Sbjct: 646  MGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSIL 705

Query: 61   WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWIPKGVKEVLQGKRVADILD-DSGVWK 120
            WGR L +KG  WRIGN   V+I  D W+ ++          +    RV+ ++D + G W+
Sbjct: 706  WGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQ 765

Query: 121  EDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLA-INNENQSLAS 180
             D++RD F   +   ILS+P  R  ++D +IW  +  G+++++  Y +A +NN      S
Sbjct: 766  GDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPS 825

Query: 181  ESNPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPE 240
             S+    +  WN  W + +  +IK+ +W+   + LPT  N+ K GV     C+FC    E
Sbjct: 826  SSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGE 885

Query: 241  DSEHLFWNCKVAKAGEDGARWTIGIMLLRFLIIQKSLEL--------------------- 300
            DS HLFW CK A+A    +++     L  FLI+++S E                      
Sbjct: 886  DSIHLFWICKFAEALWINSKFG---KLSPFLILRESHESLSKADFEELCVVIWGLWNQRN 945

Query: 301  AKLYGPSGTLRIRIGGKNYQAASK---------------SETHPSHDKWKPPDATHWKLD 360
            A+ +  S     +IG +  + A+K                 T+ +   W+PPD   +K++
Sbjct: 946  ARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAEILWQPPDEGIYKIN 1005

Query: 361  ADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRL 402
             DA +       G+G  I +  G ++ A  K + N +++ M EA A  EGL+  ++    
Sbjct: 1006 TDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASE---- 1065

BLAST of Lcy04g003210 vs. NCBI nr
Match: XP_023914298.1 (uncharacterized protein LOC112025844 [Quercus suber])

HSP 1 Score: 213.8 bits (543), Expect = 3.5e-51
Identity = 150/500 (30.00%), Postives = 236/500 (47.20%), Query Frame = 0

Query: 1    MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
            M F++L  FN AMLAK  WR++++ DSL  K  K K+F  G+ L AKEG  +F  WK+IL
Sbjct: 858  MGFKELQKFNDAMLAKQVWRLLENKDSLFHKFFKAKFFPNGSILDAKEGLGSF-AWKSIL 917

Query: 61   WGRSLFEKGYIWRIGNDKQVYIDQDPWL-IDKGGWIPKGVKEVLQGKRVADILD-DSGVW 120
             GR++  KG  WR+GN   + I +D WL   +   +   +  +    RV+ ++D D   W
Sbjct: 918  KGRAVIVKGLQWRVGNGAAIGIYRDAWLPPPQSSKVISPLNSLDIDARVSVLIDHDRKCW 977

Query: 121  KEDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLAS 180
             E +I + FL SD   I ++P +    DD V W  +P GIF++K  Y L + +E     +
Sbjct: 978  NEGVIDNTFLPSDASRIKAIPLSLTNCDDCVFWPRNPNGIFSVKSGYKLLMESELDDFLT 1037

Query: 181  ESNPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPE 240
             S+ S SK++W  IW+L +  R+K  +W+A  ++LPT  N+RK  +     C  C    E
Sbjct: 1038 TSDQSMSKKVWKGIWSLRIPNRVKSLMWRAGLDSLPTRANLRKRRLINEDTCPHCNLNSE 1097

Query: 241  DSEHLFWNC-------KVAKAGEDGARWTIGIMLLRFLIIQKSLELAKLYGPSGTL---- 300
             S H  W+C       KV         W    +L  F +  +S +L  L+    +L    
Sbjct: 1098 SSLHALWSCPSLLPIWKVHFEWLIKDSWNCRSLLDVFQLCLESSDLLDLFAMISSLIWAR 1157

Query: 301  --RIRIGG----------------KNYQAAS----KSETHPSHDKWKPPDATHWKLDADA 360
              ++R+G                 + ++ AS    +S    S  KW PP     K++ D 
Sbjct: 1158 RNQLRVGESAAPLDRICSMAVANLQEFRRASPPPLRSTPSVSPAKWTPPPLGWMKINFDG 1217

Query: 361  VWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRLGGL 420
              F +    G+G  IR+ +G ++ A  + I    +++M+E  A R  +    +      L
Sbjct: 1218 ATFAEKGLAGLGAVIRNEKGLVMAAYTQAIPLPTSVEMVEVLAARSAISLARE------L 1277

Query: 421  RSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCPRVGNFLEY 465
            + ++ L VE DS  I+N LN           ++ ++         ISFS   R+GN L +
Sbjct: 1278 QFDRVL-VEGDSEVIINALNRGGFSASSFGHIIRDINLSASAFQNISFSHTRRLGNKLAH 1337

BLAST of Lcy04g003210 vs. NCBI nr
Match: KAA3485159.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 213.0 bits (541), Expect = 5.9e-51
Identity = 141/485 (29.07%), Postives = 226/485 (46.60%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           M FRDL+ FNKA+LAK +WR++  PD L+AKV K +YF    FL A  G      W++I 
Sbjct: 241 MGFRDLSFFNKALLAKQAWRLLTQPDCLLAKVYKARYFPFTNFLSASVGSYPSFTWRSIC 300

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLI--DKGGWIPKGVKEVLQGKRVADILDDSG-V 120
             R L + G IWRIGN K V I  DPWL+  D    + + +   +Q   V  ++DDS   
Sbjct: 301 GARELIDNGLIWRIGNGKMVNIWNDPWLLGNDNNRLLVQNMN--IQWTTVNHLIDDSSFT 360

Query: 121 WKEDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHLAINNENQSLA 180
           WKED+I     S   + ILS+P  R   +D ++W  D KGI+T+K  Y + I + +Q+++
Sbjct: 361 WKEDVILKIAESDQANRILSIPLARPDAEDILVWRYDNKGIYTVKSGYRVLITSRSQTIS 420

Query: 181 SESNPSPS-KRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSK 240
           +  + +   K  +  +W L +  +IK+ +W+ + N +P L N+ K  + T  +C  C++ 
Sbjct: 421 NTMDDTGKYKSFYKQLWELQLPSKIKVHMWRLIKNYIPHLCNLHKRKLTTEVVCPLCKAG 480

Query: 241 PEDSEHLFWNC----------------------------KVAKAGEDGARWTIGIMLLRF 300
           PED+ HL W+C                             +  A  D  R  + I L   
Sbjct: 481 PEDANHLMWSCDFVRRLWKLLDLYAGLGNSTSEGKDQLVSIFTAMTDEKRKLLSISLWAI 540

Query: 301 LIIQ-KSLELAKLYGPSGTLR-IRIGGKNYQAASKSETHPSHDK---WKPPDATHWKLDA 360
             ++ K +   + +    T+R I+      Q + KS+  P   K   W+PP     KL+ 
Sbjct: 541 WFMRNKLIHEGRKFSMQETVRFIQRYVHELQMSCKSDFTPDTKKAIFWRPPPVDFVKLNF 600

Query: 361 DAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRLG 420
           DA +  +S S       R+ EG ++GA   +        + EA+A    +    D     
Sbjct: 601 DASFDKESKSAITAVVARNDEGLIMGACVYQCTEVADAFVAEARACERAILFAMD----- 660

Query: 421 GLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLELVGKMNVISFSSCPRVGNFL 449
                Q + +E DS  ++  L     D      +++ + ++   +  +SFS  PR GN  
Sbjct: 661 --MGFQKIILEGDSLTVIKKLRSAKNDRSVIRSIIQNICKIATLVEEVSFSFSPRKGNQA 716

BLAST of Lcy04g003210 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 7.5e-36
Identity = 127/513 (24.76%), Postives = 214/513 (41.72%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           + F+D+  FN A+L K  WR++  P+SLMAKV K +YF     L A  G +   VWK+I 
Sbjct: 56  IGFKDIEAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIH 115

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKGGWI--------PKGVKEVLQGKRVADIL 120
             + +  +G    +GN + + I +  WL  K            P+    V    +V+D++
Sbjct: 116 ASQEILRQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLI 175

Query: 121 DDSG-VWKEDLIRDAFLSSDVDSILSMPRNRIPKDDEVIWGTDPKGIFTIKGAYHL--AI 180
           D+SG  W++D+I   F   +   I  +        D   W     G +T+K  Y +   I
Sbjct: 176 DESGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQI 235

Query: 181 NNENQSLASESNPSPSKRLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPM 240
            N+  S    S PS +  ++  IW     P+I+  +WK L+N+LP    +    ++    
Sbjct: 236 INKRSSPQEVSEPSLNP-IYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESA 295

Query: 241 CWFCRSKPEDSEHLFWNCKVAKAG--------EDGARWTIGIMLLRFLII---------Q 300
           C  C S  E   HL + C  A+            G  W   I +  + +          +
Sbjct: 296 CIRCPSCKETVNHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWE 355

Query: 301 KSLELA-----KLYGPSGTLRIRIGGKNYQA-------------------ASKSETHP-- 360
           K+ +L      +L+     L  R  G+ + A                   A    T P  
Sbjct: 356 KASQLVPWLLWRLWKNRNELVFR--GREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQV 415

Query: 361 ---SHDKWKPPDATHWKLDADAVWFDDSHSDGVGWAIRDSEGSLIGAGCKKIRNKEAIKM 420
              S  +W+PP     K + DA W  D+   G+GW +R+ +G +   G + +   +++  
Sbjct: 416 NRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLE 475

Query: 421 LEAKAIREGLEKLTDRVRLGGLRSNQALEVESDSPEIVNLLNEKAEDLPETTFVVEEVLE 457
            E +A+R  +      + L   + N  +  ESDS  ++ +LN   E  P     ++++  
Sbjct: 476 AELEAMRWAV------LSLSRFQYNYVI-FESDSQVLIEILNND-EIWPSLKPTIQDLQR 535

BLAST of Lcy04g003210 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 108.6 bits (270), Expect = 1.5e-23
Identity = 108/465 (23.23%), Postives = 185/465 (39.78%), Query Frame = 0

Query: 33  LKGKYFSGGAFLKAKEGCKAFIVWKNILWGRSLFEKGYIWRIGNDKQVYIDQDPWLIDKG 92
           +K +YF   + L AK   +    W ++L G +L +KG    IG+ + + I  D  ++D  
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDN-IVDSH 60

Query: 93  GWIPKGVKEVLQGKRVADILDDSG---VWKEDLIRDAFLSSDVDSILSMPRNRIPKDDEV 152
              P   +E  +   + ++ +  G    W +  I      SD   I  +   +  K D++
Sbjct: 61  PPRPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKI 120

Query: 153 IWGTDPKGIFTIKGAYHLAINNENQSLASESNPSPSKRLWNFIWNLDVWPRIKITIWKAL 212
           IW  +  G +T++  Y L  ++ + ++ + + P  S  L   IWNL + P++K  +W+AL
Sbjct: 121 IWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRAL 180

Query: 213 NNALPTLENIRKLGVNTNPMCWFCRSKPEDSEHLFWNCKVAKAG---EDGARWTIGIM-- 272
           + AL T E +   G+  +P C  C  + E   H  + C  A       D +     +M  
Sbjct: 181 SQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSN 240

Query: 273 --------LLRFLIIQKSLELAKLYGPSGTLRI------------RIGGKNYQAASKSET 332
                   +L F+      +  KL       RI            R        ++K+ET
Sbjct: 241 DFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAET 300

Query: 333 H------PSHDK--------------WKPPDATHWKLDADAVWFDDSHSDGVGWAIRDSE 392
           H       SH K              W+ P AT+ K + DA +         GW IR+  
Sbjct: 301 HDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHY 360

Query: 393 GSLIGAGCKKIRNKEAIKMLEAKAIREGLEKLTDRVRLGGLRSNQALEVESDSPEIVNLL 450
           G+ I  G  K+ +       E KA+   L++         +R    + +E D   ++NL+
Sbjct: 361 GTPISWGSMKLAHTSNPLEAETKALLAALQQT-------WIRGYTQVFMEGDCQTLINLI 420

BLAST of Lcy04g003210 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 72.8 bits (177), Expect = 8.9e-13
Identity = 31/91 (34.07%), Postives = 56/91 (61.54%), Query Frame = 0

Query: 1   MSFRDLAMFNKAMLAKLSWRIIKSPDSLMAKVLKGKYFSGGAFLKAKEGCKAFIVWKNIL 60
           + FRDL  FN+A+LAK S+RII  P +L++++L+ +YF   + ++   G +    W++I+
Sbjct: 57  LGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSII 116

Query: 61  WGRSLFEKGYIWRIGNDKQVYIDQDPWLIDK 92
            GR L  +G +  IG+     +  D W++D+
Sbjct: 117 HGRELLSRGLLRTIGDGIHTKVWLDRWIMDE 147

BLAST of Lcy04g003210 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.7e-08
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 0

Query: 179 ESNPSPSK-RLWNFIWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKP 238
           E  P P K  +   IW L   P+IK  +WK L+ AL T +N+++  +  +P C  C  + 
Sbjct: 4   EIQPPPGKAEIKAKIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQED 63

Query: 239 EDSEHLFWNCKVAK 252
           E S+HLF++C  A+
Sbjct: 64  ETSQHLFFDCFYAQ 77

BLAST of Lcy04g003210 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 57.0 bits (136), Expect = 5.0e-08
Identity = 25/60 (41.67%), Postives = 35/60 (58.33%), Query Frame = 0

Query: 192 IWNLDVWPRIKITIWKALNNALPTLENIRKLGVNTNPMCWFCRSKPEDSEHLFWNCKVAK 251
           IW+L + P+IK+ IWKALNNALP    +    ++  P C  CR   E   H+ +NC  A+
Sbjct: 9   IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCRDF-ETITHILFNCPFAQ 67

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P932951.2e-1134.07Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DAR47.3e-5530.98uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A1S8AC011.4e-5330.06Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1[more]
A0A1S8ACU25.2e-5328.95Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1[more]
A0A2N9FFZ21.3e-5129.18Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A5B6WTC72.9e-5129.07Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_007180 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_006491472.14.8e-6130.28uncharacterized protein LOC102626455 [Citrus sinensis][more]
XP_024037590.11.8e-5528.77uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_022150918.11.5e-5430.98uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_023914298.13.5e-5130.00uncharacterized protein LOC112025844 [Quercus suber][more]
KAA3485159.15.9e-5129.07reverse transcriptase [Gossypium australe][more]
Match NameE-valueIdentityDescription
AT4G29090.17.5e-3624.76Ribonuclease H-like superfamily protein [more]
AT3G09510.11.5e-2323.23Ribonuclease H-like superfamily protein [more]
ATMG00310.18.9e-1334.07RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G25270.11.7e-0836.49Ribonuclease H-like superfamily protein [more]
AT3G26855.15.0e-0841.67RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 159..251
e-value: 3.7E-18
score: 66.0
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 324..449
e-value: 9.4E-13
score: 48.1
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 317..453
e-value: 3.1E-6
score: 29.2
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 52..369
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 52..369
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 324..449
e-value: 7.28887E-13
score: 63.4872

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g003210.1Lcy04g003210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity