Lcy02g019180 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g019180
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionfatty acid amide hydrolase
LocationChr02: 46395956 .. 46397135 (-)
RNA-Seq ExpressionLcy02g019180
SyntenyLcy02g019180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCAAATATCTATGTGTTACGAATTTAGAAGAAGAACCTGTTATATCCACCCTTGATGAAGATGGGAAACCTAAAGACCGAGTGGACATAGCTTTGAAGTGTCTCCCACATTATGATCCCTCTGCCCATGTTAAGAGAGACCCGTCTTCACCATTTAGATATTGGAAAATTCGTGACTATGCACATGCTTATCGGTCGAGGTTGGTGACCCCTTCCATGGTATTGTATAACTAGCTTCTTGCTGCTTGAATTTTGTTCATTTGTAGTCAACTTTCCTCACCAACTGAACTAACTATTTTGTTTCCACTATGACATTGTATTAGGTCGCAGAACAAATAATCTCAGTTATACAGGAGTTCAATCACAAAAAACCACCATCACCTCTATTGATTTCTTTTGATCCTGAGGAAGTCATGAAGCAAGCCAGCTGCTTCTACACTGAGGTTTGAGGAAGGTATTGTGGCTCATCCTTCGTATCGTTTTTGCACATTTCTGTTTTCTTTTATTCCTTTCCCTGTGATAAGAGACGGTTTAAAATAAGTTGCAATAGTCACTGATTTGTTTTGGTTTTTCAGGAAATCCTTTGTCTATCTTGGATGGGATATTCATAGCAATCAAGGATGATATAGACTGTTATCCTCATCCAACTAAAGGTTAACAATCTTGGGCAAAACTAAAGGTCTTCCATGAAGTGTTGTAGGTTAAGGTTTTGATTTTATGCTTACAGGTGCATCAACATGGATGCATGAGGTACGCCCTGTGAAAAAGGACGCGGATTCTGTTTCAAGGTTGCGTAGATGTGGTGTAATATTTGTAGGGAAAGCAAATATGCATGAGTTGGGTATGGGTACAATTGACAATAATCCAAATTATGGGTATGTATTGCTTATCACTGACTTGACAACGTTAATTGATGTTTGAGATGCATCTCTCAGGCATATATAATGCTATATTCCTTATCTCTGCAAATTGAATGTTGGAGGTTGCATTCTTGATTGTATGTTTGGAAATAGTCGAGTTGTTAATTATTAAATGTTACGCCTTATGATTTCATTGGGTCATTTGGGCTTGCAGAACAACGAGAAATCCTCATGCACCTGATAGGTATACAGGAGGGTCGTCCTCGGGTCCTGCAGCAATTGTTGCTTCTGGTATATGTTCTGCTGCACTTGGATAA

mRNA sequence

ATGCTCAAATATCTATGTGTTACGAATTTAGAAGAAGAACCTGTTATATCCACCCTTGATGAAGATGGGAAACCTAAAGACCGAGTGGACATAGCTTTGAAGTGTCTCCCACATTATGATCCCTCTGCCCATGTTAAGAGAGACCCGTCTTCACCATTTAGATATTGGAAAATTCGTGACTATGCACATGCTTATCGGTCGAGCAAGCCAGCTGCTTCTACACTGAGGTTTGAGGAAGGAAATCCTTTGTCTATCTTGGATGGGATATTCATAGCAATCAAGGATGATATAGACTGTTATCCTCATCCAACTAAAGGTGCATCAACATGGATGCATGAGGTACGCCCTGTGAAAAAGGACGCGGATTCTGTTTCAAGGTTGCGTAGATGTGGTGTAATATTTGTAGGGAAAGCAAATATGCATGAGTTGGGTATGGGTACAATTGACAATAATCCAAATTATGGAACAACGAGAAATCCTCATGCACCTGATAGGTATACAGGAGGGTCGTCCTCGGGTCCTGCAGCAATTGTTGCTTCTGGTATATGTTCTGCTGCACTTGGATAA

Coding sequence (CDS)

ATGCTCAAATATCTATGTGTTACGAATTTAGAAGAAGAACCTGTTATATCCACCCTTGATGAAGATGGGAAACCTAAAGACCGAGTGGACATAGCTTTGAAGTGTCTCCCACATTATGATCCCTCTGCCCATGTTAAGAGAGACCCGTCTTCACCATTTAGATATTGGAAAATTCGTGACTATGCACATGCTTATCGGTCGAGCAAGCCAGCTGCTTCTACACTGAGGTTTGAGGAAGGAAATCCTTTGTCTATCTTGGATGGGATATTCATAGCAATCAAGGATGATATAGACTGTTATCCTCATCCAACTAAAGGTGCATCAACATGGATGCATGAGGTACGCCCTGTGAAAAAGGACGCGGATTCTGTTTCAAGGTTGCGTAGATGTGGTGTAATATTTGTAGGGAAAGCAAATATGCATGAGTTGGGTATGGGTACAATTGACAATAATCCAAATTATGGAACAACGAGAAATCCTCATGCACCTGATAGGTATACAGGAGGGTCGTCCTCGGGTCCTGCAGCAATTGTTGCTTCTGGTATATGTTCTGCTGCACTTGGATAA

Protein sequence

MLKYLCVTNLEEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRSSKPAASTLRFEEGNPLSILDGIFIAIKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNPNYGTTRNPHAPDRYTGGSSSGPAAIVASGICSAALG
Homology
BLAST of Lcy02g019180 vs. ExPASy Swiss-Prot
Match: G7ISB0 (Fatty acid amide hydrolase OS=Medicago truncatula OX=3880 GN=FAAH PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-68
Identity = 135/216 (62.50%), Postives = 149/216 (68.98%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRSSK- 70
           E+E  +  LDEDG+P+ RV+ AL CLPHYDP A +  + S+ FRYWKIRDYA+AY+S K 
Sbjct: 84  EKEHSVVELDEDGRPEGRVESALNCLPHYDP-AKLWENSSATFRYWKIRDYAYAYQSRKV 143

Query: 71  -------------------------------------PAASTLRFEEGNPLSILDGIFIA 130
                                                 AAST RFE GNPLSILDGIFIA
Sbjct: 144 TPSMVAESIISMIEENGIDKPPTPLLLSFDAAEVRKQAAASTQRFESGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDC+PHP+ G STWMHEVR VKKDA  VSRLR CGVIF+GK NMHE GMGT  NN 
Sbjct: 204 IKDDIDCHPHPSTGGSTWMHEVRDVKKDAVCVSRLRSCGVIFIGKTNMHEFGMGTTGNNS 263

BLAST of Lcy02g019180 vs. ExPASy Swiss-Prot
Match: Q7XJJ7 (Fatty acid amide hydrolase OS=Arabidopsis thaliana OX=3702 GN=FAAH PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 5.0e-62
Identity = 123/216 (56.94%), Postives = 141/216 (65.28%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           E E  +  + ED  P DR++ ALKCLP YDPS  +  DP S FRYWKIRDYA+AYRS   
Sbjct: 84  EPEHDVVIVGEDESPIDRLETALKCLPQYDPSRSLHADPVSSFRYWKIRDYAYAYRSKLT 143

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                  AST RFE+GNP+S+LDGIF+ 
Sbjct: 144 TPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQGNPISVLDGIFVT 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDC PHPT G +TW+HE R V+KD+  VS+LR CG I +GKANMHELGMGT  NN 
Sbjct: 204 IKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELGMGTTGNNS 263

BLAST of Lcy02g019180 vs. ExPASy Swiss-Prot
Match: Q01N44 (Fatty acid amide hydrolase OS=Oryza sativa subsp. indica OX=39946 GN=FAAH PE=3 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.8e-59
Identity = 118/216 (54.63%), Postives = 143/216 (66.20%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           E E  +  L +D  P DRV+ AL CLP YDPS         PF YWKIRD+AHAYRS   
Sbjct: 85  EPEQGVLLLGDDRDPVDRVEEALHCLPPYDPSLRWPAGDKPPFLYWKIRDFAHAYRSGIT 144

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                  AST RF++GNP+SILDGIFIA
Sbjct: 145 TPSVVAEHIIAGVEEWSNKKPPMPMLVYFNADDLRKQAEASTKRFQQGNPISILDGIFIA 204

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDC+P+P+KGA+T+  ++R V+KDA  V+RLR+CGV+F+GKANMHELG+G   NNP
Sbjct: 205 IKDDIDCFPYPSKGATTFFDKIRSVEKDAVCVARLRKCGVLFIGKANMHELGLGVTGNNP 264

BLAST of Lcy02g019180 vs. ExPASy Swiss-Prot
Match: Q0JFH7 (Fatty acid amide hydrolase OS=Oryza sativa subsp. japonica OX=39947 GN=FAAH PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.8e-59
Identity = 118/216 (54.63%), Postives = 143/216 (66.20%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           E E  +  L +D  P DRV+ AL CLP YDPS         PF YWKIRD+AHAYRS   
Sbjct: 85  EPEQGVLLLGDDRDPVDRVEEALHCLPPYDPSLRWPAGDKPPFLYWKIRDFAHAYRSGIT 144

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                  AST RF++GNP+SILDGIFIA
Sbjct: 145 TPSVVAEHIIAGVEEWSNKKPPMPMLVYFNADDLRKQAEASTKRFQQGNPISILDGIFIA 204

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDC+P+P+KGA+T+  ++R V+KDA  V+RLR+CGV+F+GKANMHELG+G   NNP
Sbjct: 205 IKDDIDCFPYPSKGATTFFDKIRSVEKDAVCVARLRKCGVLFIGKANMHELGLGVTGNNP 264

BLAST of Lcy02g019180 vs. ExPASy Swiss-Prot
Match: Q2S4S2 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Salinibacter ruber (strain DSM 13855 / M31) OX=309807 GN=gatA PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.8e-16
Identity = 48/111 (43.24%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 78  EEGNPLSILDGIFIAIKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGK 137
           E GNP   L G+ +A+KD+I    +P    S  + +   +  DA  + RLR  G IF+GK
Sbjct: 61  ERGNPRP-LAGLVLAVKDNICIRGYPVSCGSKMLADFSSL-YDATVIDRLRDAGAIFIGK 120

Query: 138 ANMHELGMGTIDNNPNYGTTRNPHAPDRYTGGSSSGPAAIVASGICSAALG 189
            N  E  MG+ +   ++G  RNPHAP+   GGSS G AA VA+G+C AALG
Sbjct: 121 TNCDEFAMGSSNETSHFGPVRNPHAPEYVPGGSSGGSAAAVAAGLCHAALG 169

BLAST of Lcy02g019180 vs. ExPASy TrEMBL
Match: A0A6J1GL25 (fatty acid amide hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454961 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.6e-84
Identity = 166/216 (76.85%), Postives = 168/216 (77.78%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVISTLDEDGKP+DRVD ALKCLPHYDP   VK DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVISTLDEDGKPEDRVDKALKCLPHYDPIDRVKSDPSSPFRYWKIRDYAHAYRSRHT 143

Query: 71  ------------------SKPAA-----------------STLRFEEGNPLSILDGIFIA 130
                              KPAA                 ST RFEEGNPLSILDGIFIA
Sbjct: 144 TPSMVAEHIISIIQEFNHRKPAAPLLISFDPEEVMKQAATSTQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. ExPASy TrEMBL
Match: A0A6J1I9Z6 (fatty acid amide hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111470601 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 6.2e-84
Identity = 163/216 (75.46%), Postives = 166/216 (76.85%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVISTLDEDGKP+DRVD ALKCLPHYDP   VK DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVISTLDEDGKPEDRVDKALKCLPHYDPIDRVKSDPSSPFRYWKIRDYAHAYRSRHT 143

Query: 71  -----------------------------------SKPAASTLRFEEGNPLSILDGIFIA 130
                                               + AAS  RFEEGNPLSILDGIFIA
Sbjct: 144 TPSMVAEHIISIIQEFNHRKPAAPLLISFDPEEVMKQAAASAQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. ExPASy TrEMBL
Match: A0A5A7SLA4 (Fatty acid amide hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001640 PE=3 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.8e-83
Identity = 162/216 (75.00%), Postives = 168/216 (77.78%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPV+S LDEDGKP+DRVDIALKCLPHYDP AHV+  PSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVVSALDEDGKPEDRVDIALKCLPHYDPIAHVEAGPSSPFRYWKIRDYAHAYRSRRV 143

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                 AAST RFEEG+PLSILDGIFIA
Sbjct: 144 TPSMVAEQVISVIQEFNHKKPSAPLLISFDPEEVMKQAAASTRRFEEGSPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSV+RLRRCGVI VGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVARLRRCGVILVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. ExPASy TrEMBL
Match: A0A5D3C932 (Fatty acid amide hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001630 PE=3 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.8e-83
Identity = 162/216 (75.00%), Postives = 168/216 (77.78%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPV+S LDEDGKP+DRVDIALKCLPHYDP AHV+  PSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVVSALDEDGKPEDRVDIALKCLPHYDPIAHVEAGPSSPFRYWKIRDYAHAYRSRRV 143

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                 AAST RFEEG+PLSILDGIFIA
Sbjct: 144 TPSTVAEQVISVIQEFNHKKPSAPLLISFDPEEVMKQAAASTRRFEEGSPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSV+RLRRCGVI VGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVARLRRCGVILVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. ExPASy TrEMBL
Match: A0A6J1DX77 (fatty acid amide hydrolase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025386 PE=3 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.8e-83
Identity = 160/216 (74.07%), Postives = 168/216 (77.78%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVISTLDEDGKP+DRVDIA++CL HYDP++HVKRDP SPFRYWKIRDYA+AYRS   
Sbjct: 10  EEEPVISTLDEDGKPEDRVDIAVRCLMHYDPTSHVKRDPPSPFRYWKIRDYAYAYRSRLV 69

Query: 71  -----------------------------------SKPAASTLRFEEGNPLSILDGIFIA 130
                                               + AAST RF EGNPLSILDGIFIA
Sbjct: 70  TPSMVAEQIIPVIQEFNHKEPPAPLLISFDPEEVMKQAAASTQRFAEGNPLSILDGIFIA 129

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 130 IKDDIDCYPHPTKGASTWMHEVRSVAKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 189

BLAST of Lcy02g019180 vs. NCBI nr
Match: XP_038886943.1 (fatty acid amide hydrolase [Benincasa hispida])

HSP 1 Score: 324.7 bits (831), Expect = 5.2e-85
Identity = 166/216 (76.85%), Postives = 169/216 (78.24%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVIS LDEDGKP+DRVDIAL CLPHYDP AHV+ DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVISILDEDGKPEDRVDIALNCLPHYDPIAHVETDPSSPFRYWKIRDYAHAYRSRRV 143

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                 AAST RFEEGNPLSILDGIFIA
Sbjct: 144 TPSTVAEQIISVIQEFNHKKPSAPLLISFDPEEVMKQAAASTQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVQKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. NCBI nr
Match: XP_022952219.1 (fatty acid amide hydrolase [Cucurbita moschata])

HSP 1 Score: 322.0 bits (824), Expect = 3.4e-84
Identity = 166/216 (76.85%), Postives = 168/216 (77.78%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVISTLDEDGKP+DRVD ALKCLPHYDP   VK DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVISTLDEDGKPEDRVDKALKCLPHYDPIDRVKSDPSSPFRYWKIRDYAHAYRSRHT 143

Query: 71  ------------------SKPAA-----------------STLRFEEGNPLSILDGIFIA 130
                              KPAA                 ST RFEEGNPLSILDGIFIA
Sbjct: 144 TPSMVAEHIISIIQEFNHRKPAAPLLISFDPEEVMKQAATSTQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. NCBI nr
Match: XP_023554664.1 (fatty acid amide hydrolase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.6 bits (823), Expect = 4.4e-84
Identity = 163/216 (75.46%), Postives = 167/216 (77.31%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPV+STLDEDGKP+DRVD ALKCLPHYDP   VK DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVVSTLDEDGKPEDRVDKALKCLPHYDPIDRVKSDPSSPFRYWKIRDYAHAYRSRHT 143

Query: 71  -----------------------------------SKPAASTLRFEEGNPLSILDGIFIA 130
                                               + AAST RFEEGNPLSILDGIFIA
Sbjct: 144 TPSMVAEHIISIIQEFNHRKPAAPLLISFDPEEVMKQAAASTQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. NCBI nr
Match: XP_022971929.1 (fatty acid amide hydrolase [Cucurbita maxima])

HSP 1 Score: 320.1 bits (819), Expect = 1.3e-83
Identity = 163/216 (75.46%), Postives = 166/216 (76.85%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVISTLDEDGKP+DRVD ALKCLPHYDP   VK DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVISTLDEDGKPEDRVDKALKCLPHYDPIDRVKSDPSSPFRYWKIRDYAHAYRSRHT 143

Query: 71  -----------------------------------SKPAASTLRFEEGNPLSILDGIFIA 130
                                               + AAS  RFEEGNPLSILDGIFIA
Sbjct: 144 TPSMVAEHIISIIQEFNHRKPAAPLLISFDPEEVMKQAAASAQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSVSRLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVSRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. NCBI nr
Match: KAG6571887.1 (Fatty acid amide hydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 319.7 bits (818), Expect = 1.7e-83
Identity = 163/216 (75.46%), Postives = 166/216 (76.85%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           EEEPVISTLDEDGKP+DRVD ALKCLPHYDP   VK DPSSPFRYWKIRDYAHAYRS   
Sbjct: 84  EEEPVISTLDEDGKPEDRVDKALKCLPHYDPIDRVKSDPSSPFRYWKIRDYAHAYRSRHT 143

Query: 71  -----------------------------------SKPAASTLRFEEGNPLSILDGIFIA 130
                                               + AAST RFEEGNPLSILDGIFIA
Sbjct: 144 TPSMVAEHIISIIQEFNHRKPAAPLLISFDPEEVMKQAAASTQRFEEGNPLSILDGIFIA 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDCYPHPTKGASTWMHEVR V+KDADSV RLRRCGVIFVGKANMHELGMGT  NNP
Sbjct: 204 IKDDIDCYPHPTKGASTWMHEVRSVEKDADSVLRLRRCGVIFVGKANMHELGMGTTGNNP 263

BLAST of Lcy02g019180 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 238.8 bits (608), Expect = 3.5e-63
Identity = 123/216 (56.94%), Postives = 141/216 (65.28%), Query Frame = 0

Query: 11  EEEPVISTLDEDGKPKDRVDIALKCLPHYDPSAHVKRDPSSPFRYWKIRDYAHAYRS--- 70
           E E  +  + ED  P DR++ ALKCLP YDPS  +  DP S FRYWKIRDYA+AYRS   
Sbjct: 84  EPEHDVVIVGEDESPIDRLETALKCLPQYDPSRSLHADPVSSFRYWKIRDYAYAYRSKLT 143

Query: 71  ------------------SKP-----------------AASTLRFEEGNPLSILDGIFIA 130
                              KP                  AST RFE+GNP+S+LDGIF+ 
Sbjct: 144 TPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQGNPISVLDGIFVT 203

Query: 131 IKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELGMGTIDNNP 189
           IKDDIDC PHPT G +TW+HE R V+KD+  VS+LR CG I +GKANMHELGMGT  NN 
Sbjct: 204 IKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELGMGTTGNNS 263

BLAST of Lcy02g019180 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.7e-13
Identity = 44/113 (38.94%), Postives = 58/113 (51.33%), Query Frame = 0

Query: 76  RFEEGNPLSILDGIFIAIKDDIDCYPHPTKGASTWMHEVRPVKKDADSVSRLRRCGVIFV 135
           R  +G  L  L G+ I +KD+I     P+  AS  +   RP   DA +V +++  G I V
Sbjct: 98  RIAKGEELGPLAGVLIGVKDNICTQGMPSTAASRILEHYRP-PFDATAVKKIKELGGIVV 157

Query: 136 GKANMHELGMGTIDNNPNYGTTRNPHAPDRYTGGSSSGPAAIVASGICSAALG 189
           GK NM E GMG+      +  T NP    R  GGSS G AA VA+  C  +LG
Sbjct: 158 GKTNMDEFGMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLG 209

BLAST of Lcy02g019180 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 60.1 bits (144), Expect = 2.2e-09
Identity = 39/104 (37.50%), Postives = 49/104 (47.12%), Query Frame = 0

Query: 86  LDGIFIAIKDDIDCYPHPTK-GASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELG 145
           L G+  +I D  D   + T  G   W       +K A  V+ L + G   VGK  M ELG
Sbjct: 71  LSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKTAVVVTTLLKNGATCVGKTIMDELG 130

Query: 146 MGTIDNNPNYGTTRNPHAPDRYTGGSSSGPAAIVASGICSAALG 189
            G I  N +YGT  NP  PD   GG SSG A  V + +   +LG
Sbjct: 131 FGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGAELVDFSLG 174

BLAST of Lcy02g019180 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 55.1 bits (131), Expect = 7.2e-08
Identity = 38/104 (36.54%), Postives = 47/104 (45.19%), Query Frame = 0

Query: 86  LDGIFIAIKDDIDCYPHPTK-GASTWMHEVRPVKKDADSVSRLRRCGVIFVGKANMHELG 145
           L G+  AIKD  D     T  G   W+         A  VS L   G   +G   M E+ 
Sbjct: 28  LQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITIMDEMA 87

Query: 146 MGTIDNNPNYGTTRNPHAPDRYTGGSSSGPAAIVASGICSAALG 189
                 N +YGT RNP A DR  GGSSSG A  VA+ +   ++G
Sbjct: 88  YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIG 131

BLAST of Lcy02g019180 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 42.0 bits (97), Expect = 6.3e-04
Identity = 38/125 (30.40%), Postives = 59/125 (47.20%), Query Frame = 0

Query: 64  AYRSSKPAASTLRFEEGNPLSILDGIFIAIKDDIDCYPHPTKGASTWMHEVRPVKKDADS 123
           AY+ +K A   L   +G  L  L GI   +KD +    + T   ST   + + +  +A  
Sbjct: 248 AYKQAKEADDLL--SQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKD-QFLDIEAWV 307

Query: 124 VSRLRRCGVIFVGKANMHELGMGTIDNNPNYGTTRNPHAPDRYTGGSSSGPAAIVASGIC 183
             RL+  G + V K      G    D+    G TRNP   + ++ GSS+GPAA  ++G+ 
Sbjct: 308 YKRLKASGAVLVAKL---VTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMV 366

Query: 184 SAALG 189
             A+G
Sbjct: 368 PFAIG 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
G7ISB01.6e-6862.50Fatty acid amide hydrolase OS=Medicago truncatula OX=3880 GN=FAAH PE=1 SV=1[more]
Q7XJJ75.0e-6256.94Fatty acid amide hydrolase OS=Arabidopsis thaliana OX=3702 GN=FAAH PE=1 SV=1[more]
Q01N441.8e-5954.63Fatty acid amide hydrolase OS=Oryza sativa subsp. indica OX=39946 GN=FAAH PE=3 S... [more]
Q0JFH71.8e-5954.63Fatty acid amide hydrolase OS=Oryza sativa subsp. japonica OX=39947 GN=FAAH PE=1... [more]
Q2S4S21.8e-1643.24Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Salinibacter ruber (strain DSM ... [more]
Match NameE-valueIdentityDescription
A0A6J1GL251.6e-8476.85fatty acid amide hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454961 PE=3 SV... [more]
A0A6J1I9Z66.2e-8475.46fatty acid amide hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111470601 PE=3 SV=1[more]
A0A5A7SLA41.8e-8375.00Fatty acid amide hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A5D3C9321.8e-8375.00Fatty acid amide hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1DX771.8e-8374.07fatty acid amide hydrolase isoform X2 OS=Momordica charantia OX=3673 GN=LOC11102... [more]
Match NameE-valueIdentityDescription
XP_038886943.15.2e-8576.85fatty acid amide hydrolase [Benincasa hispida][more]
XP_022952219.13.4e-8476.85fatty acid amide hydrolase [Cucurbita moschata][more]
XP_023554664.14.4e-8475.46fatty acid amide hydrolase [Cucurbita pepo subsp. pepo][more]
XP_022971929.11.3e-8375.46fatty acid amide hydrolase [Cucurbita maxima][more]
KAG6571887.11.7e-8375.46Fatty acid amide hydrolase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G64440.13.5e-6356.94fatty acid amide hydrolase [more]
AT3G25660.15.7e-1338.94Amidase family protein [more]
AT5G09420.12.2e-0937.50translocon at the outer membrane of chloroplasts 64-V [more]
AT1G08980.17.2e-0836.54amidase 1 [more]
AT5G07360.16.3e-0430.40Amidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 56..188
e-value: 2.5E-38
score: 134.3
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 71..188
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 77..188
e-value: 5.9E-30
score: 104.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..166
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..170
NoneNo IPR availablePANTHERPTHR11895:SF164FATTY ACID AMIDE HYDROLASEcoord: 11..67
NoneNo IPR availablePANTHERPTHR11895:SF164FATTY ACID AMIDE HYDROLASEcoord: 70..188
IPR000120AmidasePANTHERPTHR11895TRANSAMIDASEcoord: 11..67
IPR000120AmidasePANTHERPTHR11895TRANSAMIDASEcoord: 70..188

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g019180.1Lcy02g019180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070291 N-acylethanolamine metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0047412 N-(long-chain-acyl)ethanolamine deacylase activity
molecular_function GO:0003824 catalytic activity