Lcy02g015360 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g015360
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectinesterase
LocationChr02: 42161971 .. 42167963 (+)
RNA-Seq ExpressionLcy02g015360
SyntenyLcy02g015360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCTCTCTCTCTCTCTCTCTCTCTCTAAAATGGCATTCTTCATTAACTCGTTTTTCTTCATTTCCATTTCCAGTACTTTCTTTCTTCTTCTATCGACCTCTCCCCCGGCCGCTGCCGCCGCCGCAGAGCACCGCCGTCACAAGTGGGTCGGACCGACCGGCCACCGTCTTATCAAAGTGGACGTTAACGGCTCCGGCGAGTTCCTCTCCGTCCAAGCCGCCGTCGATGCAGTCCCCGATTACAACACCGTCAACACCCTCATCCGAATCGGCCCTGGATATTACGTGTAATTTTCCTCAAACTCAAATTAAACCACTCATTATTTTTTTCATACTAATCATTTACAATTATGAAAATATATTTCATCATTTAATTATTTCTTTCGCTTTACGTATAAATCTTTCCCCCTGCCTTCGAAAATAATCTTCTGCCTTTTTTAAGCTAATCTTGGGGATTTTATTATTTATTATTCGTTTTTTTTAATACACCTTTTTTTTTGTCTATATAATTCTTAATTCTTTTTTAACGGGAAGAAAATGATATTGCATAAATTCAAAAACATTTCTCATTCACCTTGATATTTTTCTACATACTACTAGTAAAACATCGACAACTAAGAGAGAAAAAGTAAAAAATAGAATAAAATTATCTCTCTTAATCGTCACACTTATATATTGGTGGTGTAGAGTAGTATTGCTCTCCTAAGAATTGGGATATAAAAAATTATTTTTCCCACTATTAAAATTTCAACAAGAGTTTATTTTAATTTTTAAAGGAAGTATTTTTCTAAAAAAAAAAATTAAAGGAAGTATCATATTTCTTCTCTCACCTTCTTTTTGAATCTTCTAAAATTTTAGTAGTGAGCAATCAAAAGTACTATCCACCTCTTAATCTTTATATTATTTTTTTAGCTTAATAAATAAAGGGTAAGACTTATCTACAATAATTAGGTATCTTTCTACTATCCACCGTTAAAAGTCGTACGCTGTTTATTTTTTTTTTTAAAAAAAAGAAATATTTCTTCTCTCTTCCATTTGCACATTCGTCTTTTTGAATTATCAAAAATATAATGGTGAATATTGACTACAAAGAAGCCTTCGAGTTATACCTAAATGTTGGACGTCGTAGAACCTTTTATTGAAGATCCGTTACTCCACTAATTGTCAATTGGTTTTGAGATGGAACTTCATGTAATCTAATACCTTCAACAATAGAGATGATAATAGGTTGTTAATTGATCCATTGCTCCAATTTTTTTAGTTCAAATGTAGGGGAGAAGAATTTGAGTCTTCTCGACCATATATCCAATGTTGTTTTGACTAATTGAACTATGTTTATTTTTGCTAGTTATGTGTGTTGATTCTATTTTAAATTATCGATGAACCTAAAGTTTTTTATTTATTTATTTATTTATTTTTTGGTAGGGAGAAGGTGGTGGTTCCGGCGACGAAGCCGTACATAACATTTGAAGGAGCGGGAAAAGAGAGGACGGTGATAGAATGGCACGACCGGGCAGGTGACCGTGGTCCCAGCGGGCAGCAGCTTCGTACTTACAGAACAGCCTCTGTGACAGTTTTTGCCAATTACTTCTCTGCCCGAAACATCAGCTTCAAGGTAAATACTACTACCCCACCACAATTTCCAACTTGGCTGCACGTGAACTTCCTTCTTTCCAACACAAACCTAAAAACTCTCTCACTCGAACCAACAAAGATACTTGACTGCATATCGGTATGGTTAGATATCTTTCTACATATCATCATCAAAATATTTACAATTTTAGATGAGAAAGTTAATTTTCCGTTTTCTATCTTTCCTTTTCTTATTTGTCAATGTTTTAATTGTGGGATGTAGAAGTTACCTACGACGGATATGCATGTAAGTATGGTTCAAAATCAAATACCTCCGACATGTACCTCTCATCGTTCTCTTTCAAATTTCTTTTTTCTTACCGTTTTCGAAAATTAATTTAATTAATATGGTTTTTTAAACTATTTTTTAAAGAATAGTATGGGGAAAATGGAATTAGGTTAAAGGTGTGTTTCTTCAATCCCTAACCTTTTAACAATTTCCATCATGTTCTTCTTGTTCCTTAATGTTAAAAATGATTGTCAACTAATACTTATTACCTAATGAATTTGTATGAATTAGGAGAAAGAATTAATTAGGCAATTTGAGTGAGGAAATTTTTTTATTCTTCCACTAAAATTTTAAGAAGCTTGACAAAGACAGACTTTAAGTAGCTTAATTTCTTCTATAAATTTCTACAAACTCACTATATCGTTGTTAAAAAGTCGTCATCAACATATTTACATTTATCACCAAGAAAGCTACAATTGAAAAATATTAAGACTTAAAGGGTTAAAATAAAAATATTTTAATTAAACAATAGACATTAATTAAAGGAAAAAACTTGTCTGCATACCGGTATGTAGGGATCTCCTTGCATACCACCATTAAAACGTCGACAACGAAGAGAGATAACAAAAAATTCTCATTTTCTCCCTTCATCTTTCTTACTTGTCAAGATTTTAATGGGGTGTGTAGGGAGATCCCTACATACCGGTATGCAAGCCAGTTTTGTTCCTAATTAAAAAGAAAGGAAAAAGAAAAAGCTTAATATTAGGGATATAAATATTTGTTAGCTATGGGTTTATTACATCAATTTATACTTGTAAAAGCATAGTTTTGATTCAAGGAAAAAGAAACAAAAAAAACAAAATTATTTACTACTGTCAATTGTTTTCTCTGATTTTGTTCTGAAAATTTTGTAGTCTTTTCCCATATGTTTCTTTTAACTTTTATTAAAAAAAAAAAAAAACAAAAACAAAAAGGTACTTTTATCTTCTTGAAATTAGATAATGGTTTTAATAATAAAATGCATTTTATAAATTAAGAGGTCCTTATGAATTAGGAATCTTAGTCAGACTCATAGAAGACAACACTAGCCTTCTTTTACCAGTACCAAGTGCTTTTTTATTTAATATATAATTAACACGATATTTAATAATTAAATGGAGTCTACAAAATATTTTTTTTATTCAATAAAAATAATTTACTTTACTAAAAAAAAAGTTTCTGATTAACGTGGAAACAAAATCTATAAATAAATAAGAACCTCGAACCTATGTTGGTTGATTTCAATTATACCCCTTAACTTTCAAAAGTTCCATTTTAATTCCTTGGAATTTGAAGTTTGTTTCAAAATGACCATTTGAGACATTTTTCGTTAAATTTGTTGACAAAAAATGATGTGTTTACATGTTTATTTATTGTTTTTTTCACGAATTTGTTCAAATCTATTCATAAGTAACAATATATATGTAAACACGTCATTTTTCGTCCATGAATTGAATGAAAAAAAAGTCTTCAAGGATCGTTTTGAAACAAACTTCAAATTTCAAGAACTAAAATAAAACTTTTGAAAGTTCAATAATATAATTGAAACCACTCCCATTGTTCAGGAGTATTTTTTATAATTTAACTATTTATTTATTTACATAGATATCTTCCTACATTCGACCATAAAAAAATAATAATTAAAAAAAGAACTCTTTTAATGCGTTGGTAATTGAAAATTAGATTGATCCTAAAGGGTTAGAAAAAGCAAAAAAACAAAAAAGTGATGACCAAAAAGTATATGTATAAAAATTTTGTTCGTTTCAAAAAAGAAAAAGAAAAAGAGAATTGTGATGTTGTATCTGAGATATGTATTCACGTGACGGCAGAATACAGCACCGGCGCCGATGCCCGGAATGCAAGGGTGGCAGGCGGCGGCGTTCCGCATTTCCGGCGACAAAGCGTACTTTTCGGGCTGTGGATTCTACGGCGCACAAGATACGCTGTGTGACGACGCCGGCCGCCATTACTTCAAAGAATGCTACATCGAAGGCTCCATTGATTTCATCTTTGGAAATGGCCGTTCCATGTATAAGGTAAATCTATTTCCACTTTTCACCTTTTCTTTCATTTTACTTTTTTTAAATATCTACTTTTCATCAACCATTTCTATTTTAAAGGTTAAGGTGGAAAATATTTTAAAAGAAATAAACCTCAACAAGTATAACTTAACTGGCATAAAATATATATCCTCAAACCAAGAGATTTGAGGTTCAAATCTCTCGTCCTAAAAATTGTAAATTGTAAATTGTAAATAATAATAATAATAATAATAATAAATCTCTTAACTAATAAATGATGTTCATGTTCGTCCGAGTGTGTTTTAATATGGTGGCAAATATTATTAAGGGTTAACTTTTTGAAAAGTAACTTTTATTAATAGAGTTGTTTTGAAACTAATGATTAAAATTGGCATATTTTTTTTTTTTGAAAAAGATTAAAATAGGCAATTTAACTAATGGGATAACAATAAATTGTGGGGTCAAACTCACCTAAACTTCTTTGCACCAAAAAGAAAACTTACCTAACCTATGTTAAAATAATATGTCACTATCAAAATTATCTATTTTTATCATTAGTTTAAAAGGCGGCTCTATTTTAAAAACTGTATATTCCTATAATTAGCCTCGTCAGATAATAGAATATATTAGAACTTTTTAATTAAAATTAAAAACAAAATCCTTAAACGGTGACGTTTCCCGCCTTTTCCTTTGTGGTGTAGGATTGCGAGCTGCACTCAATCGCCACCCGGTTCGGTTCCATAGCGGCCCAAGATAGAAACTCGCCGTACGAGAAGACCGGGTTCGCTTTCGTCCGGTGCAAAGTGACCGGTTCAGGTCCCCTGTACGTGGGCCGGGCCATGGGCCAGTACTCTAGAATCGTTTACGCGTATACTTACTTTGATGACGTGGTGGCCCACGGTGGATGGGATGACTGGGACCACGTCAGCAACAAAAACAAGTAAAACAGCCACCCGTTCCCTTCTCTTCTGTTTCTTCAATTACTTGTTATTTGCATAATTATGGGCCTGGGCCGTGGTCTTAAGGTCAGTATTATTGGGCCTGGCCCACAAGGACAAAGTAGGCTTTTTATTTTCCAAAAAATGACCCTTGAAATTATATTGATTCAATTTTACCTTTTAAACAAGATGTTCTTTAGTAACTTTTGAGGGGTAATTTTGAAAGTTTCTACATGGAGGGGGGGACAAATTTAATAGTGAACTTTTCAAATGGTAATTTTGAACTGTTTTTTACTTTGATGCAGGACTGTATTTTTTGGAGTATACAAATGCTGGGGCCCAGGAGCAAGTAAAGTAAAGGGAGTGTCATGGGCCAGGGAGTTGGACTTTGAAGAGGCCCATCCATTTCTTGTGAAGAGCTTTGTCAATGGCAGGCACTGGATTGCACCCTCTGATGCTTAATCAGCTCTGCAAATACAATGCAACACTTCATATTTATTCTTTTTACATATTTTTATTCATTTCCAAATATTTATATTATACTCAATTCAATTTGTACTCGAGAATAATACTTTTGTAATATGACCAAGTCTGAAAGGACACTTTTTGATGTTTGATATTTTTTAGTATTTTACTGGTCTAAATCTCAAACTATTGACATTAACATTAACAAATAAAACTAAGGAAAAAAAAATGTGACTGCACGTCTACATGTTTACTATGGTTGAACTTGAAAATCAGACAGAATTTAGTTAGACCCTGTTAGGTATCCCAACTCAGATCACCTGCAAACAGTATGAAAATCACACACAAACTAAGGAAACACCAATCCGACACTGTTATGTTGATGCAAGGGTCAATAGACAATCATACTTCTATGATAGAAGAAGCAGGAGAAACAAAAGGAAATAACACTAGAACACCCCAGCCAATTCGAGAGCGTTGAACAAGCTCTCCACGACAATTCCTTGTCCAATATTCTACACACTGATCTTAGGCACACGATCCCCGACAAAAACGTTCATTCCCTCAGTAAACAATCGTACCCAACCAGCCCCACGGTCCCCCACACCTTTTTCTAACTGTCTGTCTCCTCTAATATTACTTTCTTGTCCTTCCTCATATATGTCTTCA

mRNA sequence

TTTCTCTCTCTCTCTCTCTCTCTCTCTAAAATGGCATTCTTCATTAACTCGTTTTTCTTCATTTCCATTTCCAGTACTTTCTTTCTTCTTCTATCGACCTCTCCCCCGGCCGCTGCCGCCGCCGCAGAGCACCGCCGTCACAAGTGGGTCGGACCGACCGGCCACCGTCTTATCAAAGTGGACGTTAACGGCTCCGGCGAGTTCCTCTCCGTCCAAGCCGCCGTCGATGCAGTCCCCGATTACAACACCGTCAACACCCTCATCCGAATCGGCCCTGGATATTACGTGGAGAAGGTGGTGGTTCCGGCGACGAAGCCGTACATAACATTTGAAGGAGCGGGAAAAGAGAGGACGGTGATAGAATGGCACGACCGGGCAGGTGACCGTGGTCCCAGCGGGCAGCAGCTTCGTACTTACAGAACAGCCTCTGTGACAGTTTTTGCCAATTACTTCTCTGCCCGAAACATCAGCTTCAAGAATACAGCACCGGCGCCGATGCCCGGAATGCAAGGGTGGCAGGCGGCGGCGTTCCGCATTTCCGGCGACAAAGCGTACTTTTCGGGCTGTGGATTCTACGGCGCACAAGATACGCTGTGTGACGACGCCGGCCGCCATTACTTCAAAGAATGCTACATCGAAGGCTCCATTGATTTCATCTTTGGAAATGGCCGTTCCATGTATAAGGATTGCGAGCTGCACTCAATCGCCACCCGGTTCGGTTCCATAGCGGCCCAAGATAGAAACTCGCCGTACGAGAAGACCGGGTTCGCTTTCGTCCGGTGCAAAGTGACCGGTTCAGGTCCCCTGTACGTGGGCCGGGCCATGGGCCAGTACTCTAGAATCGTTTACGCGTATACTTACTTTGATGACGTGGTGGCCCACGGTGGATGGGATGACTGGGACCACGTCAGCAACAAAAACAAGACTGTATTTTTTGGAGTATACAAATGCTGGGGCCCAGGAGCAAGTAAAGTAAAGGGAGTGTCATGGGCCAGGGAGTTGGACTTTGAAGAGGCCCATCCATTTCTTGTGAAGAGCTTTGTCAATGGCAGGCACTGGATTGCACCCTCTGATGCTTAATCAGCTCTGCAAATACAATGCAACACTTCATATTTATTCTTTTTACATATTTTTATTCATTTCCAAATATTTATATTATACTCAATTCAATTTGTACTCGAGAATAATACTTTTGTAATATGACCAAGTCTGAAAGGACACTTTTTGATGTTTGATATTTTTTAGTATTTTACTGGTCTAAATCTCAAACTATTGACATTAACATTAACAAATAAAACTAAGGAAAAAAAAATGTGACTGCACGTCTACATGTTTACTATGGTTGAACTTGAAAATCAGACAGAATTTAGTTAGACCCTGTTAGGTATCCCAACTCAGATCACCTGCAAACAGTATGAAAATCACACACAAACTAAGGAAACACCAATCCGACACTGTTATGTTGATGCAAGGGTCAATAGACAATCATACTTCTATGATAGAAGAAGCAGGAGAAACAAAAGGAAATAACACTAGAACACCCCAGCCAATTCGAGAGCGTTGAACAAGCTCTCCACGACAATTCCTTGTCCAATATTCTACACACTGATCTTAGGCACACGATCCCCGACAAAAACGTTCATTCCCTCAGTAAACAATCGTACCCAACCAGCCCCACGGTCCCCCACACCTTTTTCTAACTGTCTGTCTCCTCTAATATTACTTTCTTGTCCTTCCTCATATATGTCTTCA

Coding sequence (CDS)

ATGGCATTCTTCATTAACTCGTTTTTCTTCATTTCCATTTCCAGTACTTTCTTTCTTCTTCTATCGACCTCTCCCCCGGCCGCTGCCGCCGCCGCAGAGCACCGCCGTCACAAGTGGGTCGGACCGACCGGCCACCGTCTTATCAAAGTGGACGTTAACGGCTCCGGCGAGTTCCTCTCCGTCCAAGCCGCCGTCGATGCAGTCCCCGATTACAACACCGTCAACACCCTCATCCGAATCGGCCCTGGATATTACGTGGAGAAGGTGGTGGTTCCGGCGACGAAGCCGTACATAACATTTGAAGGAGCGGGAAAAGAGAGGACGGTGATAGAATGGCACGACCGGGCAGGTGACCGTGGTCCCAGCGGGCAGCAGCTTCGTACTTACAGAACAGCCTCTGTGACAGTTTTTGCCAATTACTTCTCTGCCCGAAACATCAGCTTCAAGAATACAGCACCGGCGCCGATGCCCGGAATGCAAGGGTGGCAGGCGGCGGCGTTCCGCATTTCCGGCGACAAAGCGTACTTTTCGGGCTGTGGATTCTACGGCGCACAAGATACGCTGTGTGACGACGCCGGCCGCCATTACTTCAAAGAATGCTACATCGAAGGCTCCATTGATTTCATCTTTGGAAATGGCCGTTCCATGTATAAGGATTGCGAGCTGCACTCAATCGCCACCCGGTTCGGTTCCATAGCGGCCCAAGATAGAAACTCGCCGTACGAGAAGACCGGGTTCGCTTTCGTCCGGTGCAAAGTGACCGGTTCAGGTCCCCTGTACGTGGGCCGGGCCATGGGCCAGTACTCTAGAATCGTTTACGCGTATACTTACTTTGATGACGTGGTGGCCCACGGTGGATGGGATGACTGGGACCACGTCAGCAACAAAAACAAGACTGTATTTTTTGGAGTATACAAATGCTGGGGCCCAGGAGCAAGTAAAGTAAAGGGAGTGTCATGGGCCAGGGAGTTGGACTTTGAAGAGGCCCATCCATTTCTTGTGAAGAGCTTTGTCAATGGCAGGCACTGGATTGCACCCTCTGATGCTTAA

Protein sequence

MAFFINSFFFISISSTFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA
Homology
BLAST of Lcy02g015360 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 5.1e-153
Identity = 254/351 (72.36%), Postives = 290/351 (82.62%), Query Frame = 0

Query: 3   FFINSFFFIS---ISSTFFLLLSTSPPAAAAAAEHRRH-KWVGPTGHRLIKVDVNGSGEF 62
           F ++   F+S   +   F L+ + S        +H  H KWVGP+GH++I V +NG  +F
Sbjct: 12  FSVSLLLFVSFHCLCFRFSLVAACSNSTDDQQIQHHHHRKWVGPSGHKVITVSLNGHAQF 71

Query: 63  LSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAGD 122
            SVQ AVD++P  N  +  I+I PG+Y EKVVVPATKPYITF+GAG++ T IEWHDRA D
Sbjct: 72  RSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASD 131

Query: 123 RGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSG 182
            G +GQQLRTY+TASVTV+ANYF+ARNISF NTAPAP+PGMQGWQA AFRISGDKA+FSG
Sbjct: 132 LGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSG 191

Query: 183 CGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRN 242
           CGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA+RFGSIAA  R 
Sbjct: 192 CGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGRT 251

Query: 243 SPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNK 302
            P EKTGFAFV C+VTG+GPLYVGRAMGQYSRIVYAYTYFD +VAHGGWDDWDH SNK+K
Sbjct: 252 CPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSK 311

Query: 303 TVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           T FFGVY C+GPGA+  +GVSWAR LD+E AHPF+ KSFVNGRHWIAP DA
Sbjct: 312 TAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAPRDA 362

BLAST of Lcy02g015360 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.9e-85
Identity = 146/292 (50.00%), Postives = 197/292 (67.47%), Query Frame = 0

Query: 53  NGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEW 112
           +  G+F  +Q A+D++P  N V  +I++  G Y EKV +P  K +IT EG G E+T +EW
Sbjct: 91  SNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEW 150

Query: 113 HDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGD 172
            D A      G  + TY +AS  V + +F A+NI+F+NT P P+PG  G QA A R+S D
Sbjct: 151 GDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 210

Query: 173 KAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSI 232
            A F GC   GAQDTL D  GRHY+K+CYIEGS+DFIFGN  S+Y+ C +H+IA + G++
Sbjct: 211 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 270

Query: 233 AAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH 292
            AQ R+S  E TGF+FV+CKVTG+G LY+GRA G +SR+V+AYTY D+++   GW +W  
Sbjct: 271 TAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGD 330

Query: 293 VSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWI 345
            S +  TVF+G YKC G GA+    V+WAREL  EEA PFL  +F++G  WI
Sbjct: 331 PS-REMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of Lcy02g015360 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.7e-80
Identity = 150/303 (49.50%), Postives = 199/303 (65.68%), Query Frame = 0

Query: 46  RLIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEG--- 105
           R+I VD NG G+ ++VQ AVD VPD N+    I I PG Y EKV+VP +KPYI+F G   
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 106 -AGKERTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQG 165
            AG   TVI W D+A D G  G++L TYRTASV++ +++F A  I+F+NT  A   G QG
Sbjct: 140 YAGD--TVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAE-AGEQG 199

Query: 166 WQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 225
            QA A RI GDKA F      G+QDTL DD G HYF +CYI+G++DFIFGN +S+Y+DC+
Sbjct: 200 RQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCD 259

Query: 226 LHSIATRFGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDV 285
           +HS A R+G+IAA  R+S  E TGF+FV C ++G+G +Y+GRA G YSR VY+  +  D+
Sbjct: 260 IHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADI 319

Query: 286 VAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGR 345
           +   GW DW H   + K V FG Y C G GA +   V W++ L  +E  PFL + F+ G 
Sbjct: 320 ITPVGWSDWKHPERQRK-VMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGD 378

BLAST of Lcy02g015360 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.1e-78
Identity = 150/317 (47.32%), Postives = 199/317 (62.78%), Query Frame = 0

Query: 38  KWVGPTGHR-----LIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVP 97
           KW     H+     ++ VD++G G F +VQ+A+D VPD ++  TLI +  G Y EKV V 
Sbjct: 76  KWTSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVN 135

Query: 98  ATKPYITFEGAGKERTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTA 157
             K  +  +G G + T IEW+D A   G       T  + S  VFA  F+A NISFKN A
Sbjct: 136 ENKTNLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNA 195

Query: 158 PAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGN 217
           P P PG    QA A RI GD+A F GCGFYGAQDTL DD GRH+FKEC+I+GSIDFIFGN
Sbjct: 196 PEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGN 255

Query: 218 GRSMYKDCELHSIATR-----FGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQ 277
           GRS+Y+DC ++SIA        GSI AQ R S  E++GF+FV CK+ GSG + +GRA G 
Sbjct: 256 GRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGA 315

Query: 278 YSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFE 337
           Y+ +V++ TY   ++   GW++W   S K KTV FG +KC+GPGA   + V + ++L   
Sbjct: 316 YATVVFSNTYMSGIITPEGWNNWGD-STKEKTVTFGEHKCYGPGADYKERVLFGKQLTDS 375

Query: 338 EAHPFLVKSFVNGRHWI 345
           EA  F+  SF++G  W+
Sbjct: 376 EASSFIDVSFIDGDEWL 385

BLAST of Lcy02g015360 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-72
Identity = 139/300 (46.33%), Postives = 189/300 (63.00%), Query Frame = 0

Query: 47  LIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKE 106
           ++KV +NG G F  VQ A+DA    +   TLI I  G Y E+ +V   K  +  +G G  
Sbjct: 41  VLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLVVQGMGYS 100

Query: 107 RTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAA 166
           RT IEW++             T+ + SV VF   F+A NISFKNTAPAP PG    QA A
Sbjct: 101 RTSIEWNNTTASSN------GTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAVA 160

Query: 167 FRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 226
            ++ GDKA F GCGFYG QDTL D  GRH+FK C+IEGSIDFIFGNGRS+Y+DC LHSIA
Sbjct: 161 LKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSIA 220

Query: 227 --TRFGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAH 286
                G I A  +++  ++TGF FV CK+TGS  +++GRA   Y+R++++ TY   VV+ 
Sbjct: 221 KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTYMSRVVSL 280

Query: 287 GGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWI 345
            GW+D      + +TV++G ++C+GPGA+  K V++A+ L   EA PF   SF++G  W+
Sbjct: 281 DGWNDMGDPKTQ-RTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFIDGEEWL 333

BLAST of Lcy02g015360 vs. ExPASy TrEMBL
Match: A0A6J1H1Z7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459703 PE=4 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.3e-187
Identity = 318/352 (90.34%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 1   MAFFINSFFFISISSTFFLLLSTSP---PAAAAAAEHRRHKWVGPTGHRLIKVDVNGSGE 60
           M FFI SF F    STFFLL +T P   P+A AAA HRRHKWVGPTGHRLIKVD NGSG+
Sbjct: 1   MEFFITSFLFFISISTFFLLSTTQPISSPSAPAAAPHRRHKWVGPTGHRLIKVDGNGSGD 60

Query: 61  FLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAG 120
           FL+VQAAV+AVPDYNTV+T+IRI PG YVEKVVV ATKPYITFEG GKERTVIEWHDRA 
Sbjct: 61  FLTVQAAVNAVPDYNTVSTVIRISPGCYVEKVVVAATKPYITFEGGGKERTVIEWHDRAD 120

Query: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180
           DR PSGQQLRTYRTASVTVFANYFSARNISF+NTAPAPMPGMQGWQAAAFRISGDKAYFS
Sbjct: 121 DRDPSGQQLRTYRTASVTVFANYFSARNISFRNTAPAPMPGMQGWQAAAFRISGDKAYFS 180

Query: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240
           GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR
Sbjct: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240

Query: 241 NSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300
           N P EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN
Sbjct: 241 NLPDEKTGFAFVRCKVTGSGPVYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300

Query: 301 KTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           KTVFFG YKCWGPGASKVKGVSWAREL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 KTVFFGAYKCWGPGASKVKGVSWARELEFEEAHPFLVKSFVNGRHWIAPSDA 352

BLAST of Lcy02g015360 vs. ExPASy TrEMBL
Match: A0A6J1K6P0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491639 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.3e-187
Identity = 316/352 (89.77%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 1   MAFFINSFFFISISSTFFLLLSTSP---PAAAAAAEHRRHKWVGPTGHRLIKVDVNGSGE 60
           M  F+ SF F    S FFLL +T P   P+A  AA +RRHKWVGPTGHRLIKVD NGSG+
Sbjct: 1   MESFVTSFLFFISISPFFLLSTTQPIFSPSAPVAAPYRRHKWVGPTGHRLIKVDGNGSGD 60

Query: 61  FLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAG 120
           FL+VQAAV+AVPDYNTV+T+IRI PGYYVEKVVV ATKPYITFEG GKERTVIEWHDRAG
Sbjct: 61  FLTVQAAVNAVPDYNTVSTVIRISPGYYVEKVVVAATKPYITFEGGGKERTVIEWHDRAG 120

Query: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180
           DR PSGQQLRTYRTASVTVFANYFSARNISF+NTAPAPMPGMQGWQAAAFRISGDKAYFS
Sbjct: 121 DRDPSGQQLRTYRTASVTVFANYFSARNISFRNTAPAPMPGMQGWQAAAFRISGDKAYFS 180

Query: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240
           GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR
Sbjct: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240

Query: 241 NSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300
           NSP EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN
Sbjct: 241 NSPDEKTGFAFVRCKVTGSGPVYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300

Query: 301 KTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           KTVFFG YKCWGPGASKVKGVSWAREL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 KTVFFGAYKCWGPGASKVKGVSWARELEFEEAHPFLVKSFVNGRHWIAPSDA 352

BLAST of Lcy02g015360 vs. ExPASy TrEMBL
Match: A0A6J1E808 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430254 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.3e-187
Identity = 321/352 (91.19%), Postives = 326/352 (92.61%), Query Frame = 0

Query: 1   MAFFINSFFFISISS--TFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNG-SGE 60
           M F +NSFFF    S   FFLL         AAA+H RH WVGPTGHRLIKVDVNG SGE
Sbjct: 1   MTFSLNSFFFFFFISVPAFFLL---------AAAKHHRHHWVGPTGHRLIKVDVNGISGE 60

Query: 61  FLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAG 120
           FLSVQAAVDAVPDYNTVNTLIRI PGYYVEKVVVPATKPYITFEG GKE TVIEWHDRAG
Sbjct: 61  FLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAG 120

Query: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180
           DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS
Sbjct: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180

Query: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240
           GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR
Sbjct: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240

Query: 241 NSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300
           NSP EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKN
Sbjct: 241 NSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKN 300

Query: 301 KTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           KTVFFGVYKCWGPGASKVKGVSWA+EL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 KTVFFGVYKCWGPGASKVKGVSWAKELEFEEAHPFLVKSFVNGRHWIAPSDA 343

BLAST of Lcy02g015360 vs. ExPASy TrEMBL
Match: A0A6J1I193 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468960 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 8.9e-185
Identity = 317/350 (90.57%), Postives = 323/350 (92.29%), Query Frame = 0

Query: 1   MAFFINSFFFISISSTFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNG-SGEFL 60
           MAF + SFFF  IS   F LL        AAA+H RH WVG TGHRLIKVDV G SGEF+
Sbjct: 1   MAFSLTSFFFFFISVPAFFLL--------AAAKHHRHHWVGHTGHRLIKVDVKGISGEFV 60

Query: 61  SVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAGDR 120
           SVQAAVDAVPDYNTVNTLIRI PGYYVEKVVVPA KPYITFEG GKE TVIEWHDRAGDR
Sbjct: 61  SVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPAKKPYITFEGGGKETTVIEWHDRAGDR 120

Query: 121 GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC 180
           GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC
Sbjct: 121 GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC 180

Query: 181 GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS 240
           GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS
Sbjct: 181 GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS 240

Query: 241 PYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKT 300
           P EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKN+T
Sbjct: 241 PNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNQT 300

Query: 301 VFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           VFFGVYKCWGPGASKVKGVSWAREL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 VFFGVYKCWGPGASKVKGVSWARELEFEEAHPFLVKSFVNGRHWIAPSDA 342

BLAST of Lcy02g015360 vs. ExPASy TrEMBL
Match: A0A6J1DMM2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111021954 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.8e-184
Identity = 303/320 (94.69%), Postives = 313/320 (97.81%), Query Frame = 0

Query: 30  AAAEHRRHKWVGPTGHRLIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKV 89
           +AAE RRHKWVGP+GH +I+VDVNGSGEFLSVQAAVDAVPDYNTVNTLIRI PGYYVEKV
Sbjct: 17  SAAERRRHKWVGPSGHSVIEVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKV 76

Query: 90  VVPATKPYITFEGAGKERTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFK 149
           VVPATKPYITF+G G+E TVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNI+FK
Sbjct: 77  VVPATKPYITFQGGGREVTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNITFK 136

Query: 150 NTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFI 209
           NTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYI+GSIDFI
Sbjct: 137 NTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIQGSIDFI 196

Query: 210 FGNGRSMYKDCELHSIATRFGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYS 269
           FGNGRSMYKDCELHSIATRFGSIAAQDRNSP EKTGFAFVRCKVTGSGP+YVGRAMGQYS
Sbjct: 197 FGNGRSMYKDCELHSIATRFGSIAAQDRNSPDEKTGFAFVRCKVTGSGPVYVGRAMGQYS 256

Query: 270 RIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEA 329
           RIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVY CWGPGASKVKGVSWAREL+FEEA
Sbjct: 257 RIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYNCWGPGASKVKGVSWARELEFEEA 316

Query: 330 HPFLVKSFVNGRHWIAPSDA 350
           HPFLVKSFVNGRHWIAPSDA
Sbjct: 317 HPFLVKSFVNGRHWIAPSDA 336

BLAST of Lcy02g015360 vs. NCBI nr
Match: XP_023550838.1 (probable pectinesterase 68 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.1 bits (1725), Expect = 2.1e-188
Identity = 323/350 (92.29%), Postives = 328/350 (93.71%), Query Frame = 0

Query: 1   MAFFINSFFFISISSTFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNG-SGEFL 60
           MAF +NSFFF  IS   F LL        AAA+HRRH WVGPTGHRLIKVDVNG SGEFL
Sbjct: 1   MAFSLNSFFFFFISVPAFFLL--------AAAKHRRHHWVGPTGHRLIKVDVNGISGEFL 60

Query: 61  SVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAGDR 120
           SVQAAVDAVPDYNTVNTLIRI PGYYVEKVVVPATKPYITFEG GKE TVIEWHDRAGDR
Sbjct: 61  SVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDR 120

Query: 121 GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC 180
           GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC
Sbjct: 121 GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC 180

Query: 181 GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS 240
           GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS
Sbjct: 181 GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS 240

Query: 241 PYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKT 300
           P EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKNKT
Sbjct: 241 PNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKT 300

Query: 301 VFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           VFFGVYKCWGPGASKVKGVSWA+EL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 VFFGVYKCWGPGASKVKGVSWAKELEFEEAHPFLVKSFVNGRHWIAPSDA 342

BLAST of Lcy02g015360 vs. NCBI nr
Match: XP_022958492.1 (probable pectinesterase 68 [Cucurbita moschata])

HSP 1 Score: 664.1 bits (1712), Expect = 6.8e-187
Identity = 318/352 (90.34%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 1   MAFFINSFFFISISSTFFLLLSTSP---PAAAAAAEHRRHKWVGPTGHRLIKVDVNGSGE 60
           M FFI SF F    STFFLL +T P   P+A AAA HRRHKWVGPTGHRLIKVD NGSG+
Sbjct: 1   MEFFITSFLFFISISTFFLLSTTQPISSPSAPAAAPHRRHKWVGPTGHRLIKVDGNGSGD 60

Query: 61  FLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAG 120
           FL+VQAAV+AVPDYNTV+T+IRI PG YVEKVVV ATKPYITFEG GKERTVIEWHDRA 
Sbjct: 61  FLTVQAAVNAVPDYNTVSTVIRISPGCYVEKVVVAATKPYITFEGGGKERTVIEWHDRAD 120

Query: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180
           DR PSGQQLRTYRTASVTVFANYFSARNISF+NTAPAPMPGMQGWQAAAFRISGDKAYFS
Sbjct: 121 DRDPSGQQLRTYRTASVTVFANYFSARNISFRNTAPAPMPGMQGWQAAAFRISGDKAYFS 180

Query: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240
           GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR
Sbjct: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240

Query: 241 NSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300
           N P EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN
Sbjct: 241 NLPDEKTGFAFVRCKVTGSGPVYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300

Query: 301 KTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           KTVFFG YKCWGPGASKVKGVSWAREL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 KTVFFGAYKCWGPGASKVKGVSWARELEFEEAHPFLVKSFVNGRHWIAPSDA 352

BLAST of Lcy02g015360 vs. NCBI nr
Match: KAG6579412.1 (putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 664.1 bits (1712), Expect = 6.8e-187
Identity = 321/352 (91.19%), Postives = 327/352 (92.90%), Query Frame = 0

Query: 1   MAFFINSFFFISISS--TFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNG-SGE 60
           MAF +NSFFF    S   FFLL         AAA+H RH WVGPTGHRLIKVDVNG SGE
Sbjct: 1   MAFSLNSFFFFFFISVPAFFLL---------AAAKHHRHHWVGPTGHRLIKVDVNGISGE 60

Query: 61  FLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAG 120
           FLSVQAAVDA+PDYNTVNTLIRI PGYYVEKVVVPATKPYITFEG GKE TVIEWHDRAG
Sbjct: 61  FLSVQAAVDAIPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAG 120

Query: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180
           DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS
Sbjct: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180

Query: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240
           GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR
Sbjct: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240

Query: 241 NSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300
           NSP EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKN
Sbjct: 241 NSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKN 300

Query: 301 KTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           KTVFFGVYKCWGPGASKVKGVSWA+EL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 KTVFFGVYKCWGPGASKVKGVSWAKELEFEEAHPFLVKSFVNGRHWIAPSDA 343

BLAST of Lcy02g015360 vs. NCBI nr
Match: KAG7036124.1 (putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 663.3 bits (1710), Expect = 1.2e-186
Identity = 318/350 (90.86%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MAFFINSF-FFISISSTFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNGSGEFL 60
           M  FI SF FFISIS+  F LLST+ P ++ +A HRRHKWVGPTGHRLIKVD NGSG+FL
Sbjct: 1   MESFITSFLFFISIST--FFLLSTTQPISSPSAPHRRHKWVGPTGHRLIKVDGNGSGDFL 60

Query: 61  SVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAGDR 120
           +VQAAV+AVPDYNTV+T+IRI PGYYVEKVVV ATKPYITFEG GKERTVIEWHDRAGDR
Sbjct: 61  TVQAAVNAVPDYNTVSTVIRISPGYYVEKVVVAATKPYITFEGGGKERTVIEWHDRAGDR 120

Query: 121 GPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGC 180
            PSGQQLRTYRTASVTVFANYFSARNISF+NTAPAPMPGMQGWQAAAFRISGDKAYFSGC
Sbjct: 121 DPSGQQLRTYRTASVTVFANYFSARNISFRNTAPAPMPGMQGWQAAAFRISGDKAYFSGC 180

Query: 181 GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNS 240
           GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRN 
Sbjct: 181 GFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNL 240

Query: 241 PYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKT 300
           P EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKT
Sbjct: 241 PDEKTGFAFVRCKVTGSGPVYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKT 300

Query: 301 VFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           VFFG YKCWGPGASKVKGVSWAREL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 VFFGAYKCWGPGASKVKGVSWARELEFEEAHPFLVKSFVNGRHWIAPSDA 348

BLAST of Lcy02g015360 vs. NCBI nr
Match: XP_022922190.1 (probable pectinesterase 68 [Cucurbita moschata])

HSP 1 Score: 662.9 bits (1709), Expect = 1.5e-186
Identity = 321/352 (91.19%), Postives = 326/352 (92.61%), Query Frame = 0

Query: 1   MAFFINSFFFISISS--TFFLLLSTSPPAAAAAAEHRRHKWVGPTGHRLIKVDVNG-SGE 60
           M F +NSFFF    S   FFLL         AAA+H RH WVGPTGHRLIKVDVNG SGE
Sbjct: 1   MTFSLNSFFFFFFISVPAFFLL---------AAAKHHRHHWVGPTGHRLIKVDVNGISGE 60

Query: 61  FLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAG 120
           FLSVQAAVDAVPDYNTVNTLIRI PGYYVEKVVVPATKPYITFEG GKE TVIEWHDRAG
Sbjct: 61  FLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAG 120

Query: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180
           DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS
Sbjct: 121 DRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFS 180

Query: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240
           GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR
Sbjct: 181 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDR 240

Query: 241 NSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKN 300
           NSP EKTGFAFVRCKVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKN
Sbjct: 241 NSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKN 300

Query: 301 KTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           KTVFFGVYKCWGPGASKVKGVSWA+EL+FEEAHPFLVKSFVNGRHWIAPSDA
Sbjct: 301 KTVFFGVYKCWGPGASKVKGVSWAKELEFEEAHPFLVKSFVNGRHWIAPSDA 343

BLAST of Lcy02g015360 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 542.0 bits (1395), Expect = 3.6e-154
Identity = 254/351 (72.36%), Postives = 290/351 (82.62%), Query Frame = 0

Query: 3   FFINSFFFIS---ISSTFFLLLSTSPPAAAAAAEHRRH-KWVGPTGHRLIKVDVNGSGEF 62
           F ++   F+S   +   F L+ + S        +H  H KWVGP+GH++I V +NG  +F
Sbjct: 12  FSVSLLLFVSFHCLCFRFSLVAACSNSTDDQQIQHHHHRKWVGPSGHKVITVSLNGHAQF 71

Query: 63  LSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEWHDRAGD 122
            SVQ AVD++P  N  +  I+I PG+Y EKVVVPATKPYITF+GAG++ T IEWHDRA D
Sbjct: 72  RSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASD 131

Query: 123 RGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSG 182
            G +GQQLRTY+TASVTV+ANYF+ARNISF NTAPAP+PGMQGWQA AFRISGDKA+FSG
Sbjct: 132 LGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSG 191

Query: 183 CGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRN 242
           CGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA+RFGSIAA  R 
Sbjct: 192 CGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGRT 251

Query: 243 SPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNK 302
            P EKTGFAFV C+VTG+GPLYVGRAMGQYSRIVYAYTYFD +VAHGGWDDWDH SNK+K
Sbjct: 252 CPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSK 311

Query: 303 TVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWIAPSDA 350
           T FFGVY C+GPGA+  +GVSWAR LD+E AHPF+ KSFVNGRHWIAP DA
Sbjct: 312 TAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAPRDA 362

BLAST of Lcy02g015360 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.2e-86
Identity = 146/292 (50.00%), Postives = 197/292 (67.47%), Query Frame = 0

Query: 53  NGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKERTVIEW 112
           +  G+F  +Q A+D++P  N V  +I++  G Y EKV +P  K +IT EG G E+T +EW
Sbjct: 91  SNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEW 150

Query: 113 HDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGD 172
            D A      G  + TY +AS  V + +F A+NI+F+NT P P+PG  G QA A R+S D
Sbjct: 151 GDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 210

Query: 173 KAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSI 232
            A F GC   GAQDTL D  GRHY+K+CYIEGS+DFIFGN  S+Y+ C +H+IA + G++
Sbjct: 211 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 270

Query: 233 AAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH 292
            AQ R+S  E TGF+FV+CKVTG+G LY+GRA G +SR+V+AYTY D+++   GW +W  
Sbjct: 271 TAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGD 330

Query: 293 VSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWI 345
            S +  TVF+G YKC G GA+    V+WAREL  EEA PFL  +F++G  WI
Sbjct: 331 PS-REMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of Lcy02g015360 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 4.1e-81
Identity = 150/303 (49.50%), Postives = 199/303 (65.68%), Query Frame = 0

Query: 46  RLIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEG--- 105
           R+I VD NG G+ ++VQ AVD VPD N+    I I PG Y EKV+VP +KPYI+F G   
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 106 -AGKERTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQG 165
            AG   TVI W D+A D G  G++L TYRTASV++ +++F A  I+F+NT  A   G QG
Sbjct: 140 YAGD--TVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAE-AGEQG 199

Query: 166 WQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 225
            QA A RI GDKA F      G+QDTL DD G HYF +CYI+G++DFIFGN +S+Y+DC+
Sbjct: 200 RQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCD 259

Query: 226 LHSIATRFGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDV 285
           +HS A R+G+IAA  R+S  E TGF+FV C ++G+G +Y+GRA G YSR VY+  +  D+
Sbjct: 260 IHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADI 319

Query: 286 VAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGR 345
           +   GW DW H   + K V FG Y C G GA +   V W++ L  +E  PFL + F+ G 
Sbjct: 320 ITPVGWSDWKHPERQRK-VMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGD 378

BLAST of Lcy02g015360 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.2e-79
Identity = 150/317 (47.32%), Postives = 199/317 (62.78%), Query Frame = 0

Query: 38  KWVGPTGHR-----LIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVP 97
           KW     H+     ++ VD++G G F +VQ+A+D VPD ++  TLI +  G Y EKV V 
Sbjct: 76  KWTSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVN 135

Query: 98  ATKPYITFEGAGKERTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTA 157
             K  +  +G G + T IEW+D A   G       T  + S  VFA  F+A NISFKN A
Sbjct: 136 ENKTNLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNA 195

Query: 158 PAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGN 217
           P P PG    QA A RI GD+A F GCGFYGAQDTL DD GRH+FKEC+I+GSIDFIFGN
Sbjct: 196 PEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGN 255

Query: 218 GRSMYKDCELHSIATR-----FGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQ 277
           GRS+Y+DC ++SIA        GSI AQ R S  E++GF+FV CK+ GSG + +GRA G 
Sbjct: 256 GRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGA 315

Query: 278 YSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFE 337
           Y+ +V++ TY   ++   GW++W   S K KTV FG +KC+GPGA   + V + ++L   
Sbjct: 316 YATVVFSNTYMSGIITPEGWNNWGD-STKEKTVTFGEHKCYGPGADYKERVLFGKQLTDS 375

Query: 338 EAHPFLVKSFVNGRHWI 345
           EA  F+  SF++G  W+
Sbjct: 376 EASSFIDVSFIDGDEWL 385

BLAST of Lcy02g015360 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.4e-73
Identity = 139/300 (46.33%), Postives = 189/300 (63.00%), Query Frame = 0

Query: 47  LIKVDVNGSGEFLSVQAAVDAVPDYNTVNTLIRIGPGYYVEKVVVPATKPYITFEGAGKE 106
           ++KV +NG G F  VQ A+DA    +   TLI I  G Y E+ +V   K  +  +G G  
Sbjct: 41  VLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLVVQGMGYS 100

Query: 107 RTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAA 166
           RT IEW++             T+ + SV VF   F+A NISFKNTAPAP PG    QA A
Sbjct: 101 RTSIEWNNTTASSN------GTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAVA 160

Query: 167 FRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 226
            ++ GDKA F GCGFYG QDTL D  GRH+FK C+IEGSIDFIFGNGRS+Y+DC LHSIA
Sbjct: 161 LKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSIA 220

Query: 227 --TRFGSIAAQDRNSPYEKTGFAFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDVVAH 286
                G I A  +++  ++TGF FV CK+TGS  +++GRA   Y+R++++ TY   VV+ 
Sbjct: 221 KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTYMSRVVSL 280

Query: 287 GGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSWARELDFEEAHPFLVKSFVNGRHWI 345
            GW+D      + +TV++G ++C+GPGA+  K V++A+ L   EA PF   SF++G  W+
Sbjct: 281 DGWNDMGDPKTQ-RTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFIDGEEWL 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPF35.1e-15372.36Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q8VYZ35.9e-8550.00Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9FM795.7e-8049.50Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Q9ZQA33.1e-7847.32Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q9ZQA42.0e-7246.33Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H1Z73.3e-18790.34Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459703 PE=4 SV=1[more]
A0A6J1K6P07.3e-18789.77Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491639 PE=4 SV=1[more]
A0A6J1E8087.3e-18791.19Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430254 PE=4 SV=1[more]
A0A6J1I1938.9e-18590.57Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468960 PE=4 SV=1[more]
A0A6J1DMM29.8e-18494.69Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111021954 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_023550838.12.1e-18892.29probable pectinesterase 68 [Cucurbita pepo subsp. pepo][more]
XP_022958492.16.8e-18790.34probable pectinesterase 68 [Cucurbita moschata][more]
KAG6579412.16.8e-18791.19putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7036124.11.2e-18690.86putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022922190.11.5e-18691.19probable pectinesterase 68 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G47500.13.6e-15472.36Pectin lyase-like superfamily protein [more]
AT5G19730.14.2e-8650.00Pectin lyase-like superfamily protein [more]
AT5G55590.14.1e-8149.50Pectin lyase-like superfamily protein [more]
AT2G36710.12.2e-7947.32Pectin lyase-like superfamily protein [more]
AT2G36700.11.4e-7346.33Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 50..340
e-value: 1.7E-60
score: 204.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 45..348
e-value: 4.5E-97
score: 327.3
NoneNo IPR availablePANTHERPTHR31321:SF19PECTINESTERASE 68-RELATEDcoord: 24..348
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 24..348
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 202..211
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 45..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g015360.1Lcy02g015360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity