Homology
BLAST of Lcy02g011350 vs. ExPASy Swiss-Prot
Match:
V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)
HSP 1 Score: 448.0 bits (1151), Expect = 3.3e-124
Identity = 349/1008 (34.62%), Postives = 526/1008 (52.18%), Query Frame = 0
Query: 18 YDVLNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMV 77
+ +L++R ES+ I++I + ++ + L V + VGIDS ++ M L H+ S+ V+MV
Sbjct: 162 WHLLDDRYESNQIKEITNSIFRQLK-CKRLDVGANLVGIDSHVKEMILRLHLESSDVRMV 221
Query: 78 GIYGMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAIL--KV 137
GIYG+GG+GKTT+AK +YN+++ EFE SFL N+RE S L LQ LL IL +
Sbjct: 222 GIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNP-QVLFHLQNQLLGDILEGEG 281
Query: 138 NLEVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQ 197
+ +++V ++I+D L S++V IVLDDVD L QLE L+G R+W G GS++I+TTRNK
Sbjct: 282 SQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKH 341
Query: 198 LLSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVL 257
+L+ D +++++GL+ ++A ELFS +AFK++ P S+Y +L+ R YC+GLPLAL VL
Sbjct: 342 VLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVL 401
Query: 258 GSSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDR 317
GS LC + +W+ L + ++ DI +L+ S++GL+ K+IFLD++CFF GE D
Sbjct: 402 GSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDF 461
Query: 318 IKDMLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRES--SEPEKKSR 377
+ +L+ CD GI+ L DL LIT+ N++ MHDLI+QMG IV RE+ EP K SR
Sbjct: 462 VLRILDGCDFPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIV-RENFPVEPNKWSR 521
Query: 378 LWVAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNI--- 437
LW D L + G +V+ + LDL L K V + F KM LRLL + +
Sbjct: 522 LWDPCDFERALTADEGIKSVETMSLDLSKL---KRVCSNSNVFAKMTKLRLLKVYSSSDI 581
Query: 438 --------KFSKNIFECLPNKLKWIECCRSIGFPS----------FPISS----YVNDQL 497
+ + +++ + ++ +S FPS +P+ S + +L
Sbjct: 582 DSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKL 641
Query: 498 VGLDVKHSFVERLGKILENSRRLKHVDFSGSFVLEEILDFSAALNLKELYLRDCGSLKMI 557
V L +K S +++L + ++ RLK +D S S L ++ +FS+ NL+ L L C SL I
Sbjct: 642 VELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDI 701
Query: 558 HESVGSLSKLITLDFQGCVNLKKLP-SYFMLKSLEVLNLTKCIQLEEIPDLSVASNLKEL 617
H SVG++ KL TL + C LK LP S L+SLE L L+ C + E+ P+ N+K L
Sbjct: 702 HPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPE--KGGNMKSL 761
Query: 618 RLSECSNLRIIHGSTS-RSLNKLVTLDLNHCDNVKRLPCYVMSKSLELFILTNFMIRLNL 677
+ N I S L L +L L++C ++ P K + LT ++
Sbjct: 762 TELDLKNTAIKDLPDSIGDLESLESLYLSNCSKFEKFP----EKGGNMKSLTELDLKNTA 821
Query: 678 EKDSYFSLAKFPSNLKLEYLKVLNLCGCENLEE--------------------ITDFSIS 737
KD S+ LE L++LNL C E+ I D S
Sbjct: 822 IKDLPDSIG------DLESLEILNLSDCAKFEKFPEKGGNMKSLKELDLQNTAIKDLPDS 881
Query: 738 ----SNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALNLKLLNLS 797
+L+ L+L+DC E ++ +L+ L N ++ S +LK L LS
Sbjct: 882 IGDLKSLKYLSLSDCSKFEKFPEKGGNMKRLLQLILSNTAIKDLPDSIGDLESLKYLYLS 941
Query: 798 KCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYL-KLKSLDSLNLDGCIKLEQLPEFD 857
C E G++ L L+LK ++ LP + L+SL+ LNL C K E+ PE
Sbjct: 942 DCSKFEKFPEKGGNMKSLTELDLKNT-AIKDLPDSIGDLESLEILNLSDCAKFEKFPEKG 1001
Query: 858 KNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYELCLRE 917
NMKSL+++ L T IK+LP SIG L L+YL L C+ P + +KSL +L L
Sbjct: 1002 GNMKSLKELDLQNTAIKDLPDSIGDLESLKYLYLSDCSKFEKFPEKGGNMKSLLQLILSN 1061
Query: 918 CSKLDM---FPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLNLSG 966
+ D+ SL Y S+CSK F E N+ SL L L
Sbjct: 1062 TAIKDLPDSIGDLESLEYLHLSVCSKFE------------KFPEKGGNM-KSLRELGLRN 1121
BLAST of Lcy02g011350 vs. ExPASy Swiss-Prot
Match:
V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)
HSP 1 Score: 424.1 bits (1089), Expect = 5.1e-117
Identity = 324/921 (35.18%), Postives = 477/921 (51.79%), Query Frame = 0
Query: 21 LNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMVGIY 80
L + ES+ I++I + ++ + L + VGIDS ++ M H+ S+ V+MVG+Y
Sbjct: 170 LQDGYESNQIKEITDSIFRRLK-CKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMY 229
Query: 81 GMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFN--GLVQLQENLLYAILK--VN 140
G+GG+GKTT+AK +YN+++ EFE SFL N+RE +FN G+ LQ LL ILK +
Sbjct: 230 GVGGIGKTTIAKVIYNELSREFEYMSFLENIRE---KFNTQGVSPLQNQLLDDILKGEGS 289
Query: 141 LEVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQL 200
+N+V G ++I+D L SK V IVLDDVD QLE L+ R+W G GS++I+TTRNK +
Sbjct: 290 QNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHV 349
Query: 201 LSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLG 260
L D +++++GL+ ++A ELFS +AF+++ P S+Y +LS R YC+GLPLAL VLG
Sbjct: 350 LDVQKVDDLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLG 409
Query: 261 SSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDRI 320
L + +W+S L + + +I +L+ S++GL K IFLD++CFF GED D +
Sbjct: 410 CLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFV 469
Query: 321 KDMLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRE-SSEPEKKSRLW 380
+L+ACD + GI L D LIT++ NR++MHDLI+QMG IV + EP K SRLW
Sbjct: 470 SKILDACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLW 529
Query: 381 VAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIR---NIKF 440
D L G V+ I LDL L K V + AF KM LRLL ++ +I F
Sbjct: 530 DTCDFERALTAYKGIKRVETISLDLSKL---KRVCSNSNAFAKMTRLRLLKVQSSLDIDF 589
Query: 441 SK---------NIFECLPNKLKWIECCRSIGFPSFPI--------------SSYVNDQLV 500
+++ + + R FPS+ + S++ +LV
Sbjct: 590 EPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLV 649
Query: 501 GLDVKHSFVERLGKILENSRRLKHVDFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIH 560
L +K S +++L ++ LK +D S S L ++ +FS+ NL+ L+LR C SL IH
Sbjct: 650 ELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIH 709
Query: 561 ESVGSLSKLITLDFQGCVNLKKLP-SYFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELR 620
SVG++ KL TL + C LK LP S L+SLE+L+L C + E+ P+ N+K L
Sbjct: 710 PSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPE--KGGNMKSLT 769
Query: 621 LSECSNLRIIHGSTS-RSLNKLVTLDLNHCDNVKRLPCYVMSKSLELFILTNFMIRLNLE 680
+ N I S L L LDL+ C ++ P K + L +R
Sbjct: 770 ELDLQNTAIKDLPDSIGDLESLKYLDLSDCSKFEKFP----EKGGNMKSLRELDLRNTAI 829
Query: 681 KDSYFSLAKFPSNLK-LEYLKVLNLCGCENLEEITDFSISSNLEILNLTDCIN--LRMIH 740
KD P +++ LE L+ L L C E+ + N++ L D N ++ +
Sbjct: 830 KD-------LPDSIRDLESLERLYLSYCSKFEKFPE--KGGNMKSLMELDLQNTAIKDLP 889
Query: 741 ESTRSLDKLVHCNLYNCKNLEMLPSFSAALNLKLLNLSKCIN--LRTVHESIGSLDKLVT 800
+S L+ L + +L NC E P N+K L N ++ + +SIG L+ LV+
Sbjct: 890 DSIGDLESLKYLDLSNCSKFEKFPEKGG--NMKSLTELFLENTAIKDLPDSIGDLESLVS 949
Query: 801 LELKFCYNLEKLP----------------TYLK--------LKSLDSLNLDGCIKLEQLP 860
L L C EK P T +K L+SL L L K E+LP
Sbjct: 950 LNLSDCSKFEKFPEKGGNMKSLNWLYLNNTAIKDLPDSIGDLESLMRLYLSNSSKFEKLP 1009
Query: 861 EFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYELC 880
E NMKSL + L T IK+LP SIG L L+ LSL C LP + + +
Sbjct: 1010 EKVGNMKSLELLDLRNTAIKDLPDSIGDLEPLEKLSLSNCPKFEVLPLSLKAIDAHLCTS 1066
BLAST of Lcy02g011350 vs. ExPASy Swiss-Prot
Match:
O23530 (Protein SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=SNC1 PE=1 SV=5)
HSP 1 Score: 375.9 bits (964), Expect = 1.6e-102
Identity = 304/997 (30.49%), Postives = 506/997 (50.75%), Query Frame = 0
Query: 12 ITILFTYDVLNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHP-------VGIDSQLEAME 71
+ ++ YD+ E+ +I+++ ++VL K M P VGI++ +EA++
Sbjct: 153 VAVMAGYDLRKWPSEAAMIEELAEDVLRK---------TMTPSDDFGDLVGIENHIEAIK 212
Query: 72 LVSHVVSNGVQ-MVGIYGMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGL-V 131
V + S + MVGI+G G+GK+T+ +ALY+K++ +F +F++ + +G+ +
Sbjct: 213 SVLCLESKEARIMVGIWGQSGIGKSTIGRALYSKLSIQFHHRAFITYKSTSGSDVSGMKL 272
Query: 132 QLQENLLYAIL-KVNLEVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWF 191
+ ++ LL IL + ++++ + ++ RL +KVLI+LDDVD L+ L+ LVG +WF
Sbjct: 273 RWEKELLSEILGQKDIKIEH----FGVVEQRLKQQKVLILLDDVDSLEFLKTLVGKAEWF 332
Query: 192 GRGSKIIVTTRNKQLLSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRA 251
G GS+IIV T+++QLL +H D +++++ + AL + AF + P ++ +L+
Sbjct: 333 GSGSRIIVITQDRQLLKAHEIDLIYEVEFPSEHLALTMLCRSAFGKDSPPDDFKELAFEV 392
Query: 252 TSYCKGLPLALVVLGSSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIF 311
LPL L VLGSSL R + W ++ N L DI L++S++ L K +D+F
Sbjct: 393 AKLAGNLPLGLSVLGSSLKGRTKEWWMEMMPRLRNGLNGDIMKTLRVSYDRLHQKDQDMF 452
Query: 312 LDISCFFVGEDVDRIKDMLEACDLNPNYGITILMDLSLITI-EDNRLKMHDLIRQMGQTI 371
L I+C F G +V +KD+L+ N G T+L + SLI I D ++MH+L+ ++G+ I
Sbjct: 453 LYIACLFNGFEVSYVKDLLK-----DNVGFTMLTEKSLIRITPDGYIEMHNLLEKLGREI 512
Query: 372 -VCRESSEPEKKSRLWVAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKM 431
+ P K+ L +DI ++ E +GT+ + I+L + + ID ++FK M
Sbjct: 513 DRAKSKGNPGKRRFLTNFEDIHEVVTEKTGTETLLGIRLPFEEYFSTRPLLIDKESFKGM 572
Query: 432 KNLRLLIIRNIKFSKNIFECLPNKLK---WIECCRSIGFPSFPISSYVNDQLVGLDVKHS 491
+NL+ L I LP KL+ W +C S P S++ + LV L +K+S
Sbjct: 573 RNLQYLEIGYYGDLPQSLVYLPLKLRLLDWDDC----PLKSLP-STFKAEYLVNLIMKYS 632
Query: 492 FVERLGKILENSRRLKHVDFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLS 551
+E+L + LK ++ S L+EI D S A+NL+EL L C SL + S+ + +
Sbjct: 633 KLEKLWEGTLPLGSLKEMNLRYSNNLKEIPDLSLAINLEELDLVGCKSLVTLPSSIQNAT 692
Query: 552 KLITLDFQGCVNLKKLPSYFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLR 611
KLI LD C L+ P+ L+SLE LNLT C L P ++ ++ E N
Sbjct: 693 KLIYLDMSDCKKLESFPTDLNLESLEYLNLTGCPNLRNFP--AIKMGCSDVDFPEGRNEI 752
Query: 612 IIHGSTSRSLNKLVTLDLNHCDNVKR-LPCYVMSKSLELFILTNFMIRLNLEKDSYFSLA 671
++ NK + L++ D + R +PC + L LN+ + L
Sbjct: 753 VVEDC---FWNKNLPAGLDYLDCLTRCMPCEFRPEQLAF---------LNVRGYKHEKL- 812
Query: 672 KFPSNLKLEYLKVLNLCGCENLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVH 731
+ L L+ ++L ENL EI D S ++ LE L L +C +L + + +L +LV
Sbjct: 813 -WEGIQSLGSLEGMDLSESENLTEIPDLSKATKLESLILNNCKSLVTLPSTIGNLHRLVR 872
Query: 732 CNLYNCKNLEMLPSFSAALNLKLLNLSKCINLRT--------------------VHESIG 791
+ C LE+LP+ +L+ L+LS C +LR+ + +IG
Sbjct: 873 LEMKECTGLEVLPTDVNLSSLETLDLSGCSSLRSFPLISTNIVWLYLENTAIEEIPSTIG 932
Query: 792 SLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLEQLPEFDKNMKSLRKMMLDQT 851
+L +LV LE+K C LE LPT + L SL++L+L GC L P +++K L L+ T
Sbjct: 933 NLHRLVRLEMKKCTGLEVLPTDVNLSSLETLDLSGCSSLRSFPLISESIKWL---YLENT 992
Query: 852 GIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYELCLRECSKLDMFPQRSSLN 911
I+E+ P + L+ L L C +L+ LP+ I L+ L ++EC+ L++ P +L
Sbjct: 993 AIEEI-PDLSKATNLKNLKLNNCKSLVTLPTTIGNLQKLVSFEMKECTGLEVLPIDVNL- 1052
Query: 912 YSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLNLSGNKFSCLPS-LQNFKSLK 971
S L IL+L C+ L T +S+++ L L +PS + N L
Sbjct: 1053 -------SSLMILDLSGCS-----SLRTFPLISTNIVWLYLENTAIEEIPSTIGNLHRLV 1093
BLAST of Lcy02g011350 vs. ExPASy Swiss-Prot
Match:
A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)
HSP 1 Score: 374.8 bits (961), Expect = 3.5e-102
Identity = 291/906 (32.12%), Postives = 464/906 (51.21%), Query Frame = 0
Query: 23 NRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMVGIYGM 82
N ES IQ I+K++ K + + VGI+SQ++ + + + GV++VGI+GM
Sbjct: 161 NGDESKCIQQILKDIFDKFCFS-ISITNRDLVGIESQIKKLSSLLRMDLKGVRLVGIWGM 220
Query: 83 GGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN-LEVNN 142
GG+GKTT A+AL+N+ FE FL +V+E Q + L+ LQ+ LL +LKV ++ +
Sbjct: 221 GGVGKTTAARALFNRYYQNFESACFLEDVKE-YLQHHTLLYLQKTLLSKLLKVEFVDCTD 280
Query: 143 VDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSHG 202
+ I++ RLCSKKVL+VLDDV+ DQL+KLVG DWFG GS+I++TTR+ +LL +H
Sbjct: 281 TEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHD 340
Query: 203 FDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLCN 262
+ ++I+ L++D+A+ELF+ HAFK S P + +L Y GLPLAL VLGS L
Sbjct: 341 VHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYK 400
Query: 263 RDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDRIKDMLE 322
D W S + +++ +I L+ISF+GL D K IFLDI+CFF G + + +
Sbjct: 401 EDLDVWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFH 460
Query: 323 ACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAKDII 382
A +P G+ L++ SLI I +++++MHDL+++MG+ I +ES R++ +D+
Sbjct: 461 ASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQES----PMRRIYRPEDVK 520
Query: 383 NMLKENSGTDAVKAIKLDLP--FLVQEKLVPIDPKAFKKMKNLRLLI--IRNIKFSKNIF 442
+ + +A++ + L P F E +A KK + LR+L+ N F + +
Sbjct: 521 DACIGDMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPV- 580
Query: 443 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFS 502
LPN L W+E R+ SFP S++ +LV L +K S + L + L +D S
Sbjct: 581 AYLPNSLLWLE-WRNYSSNSFP-SNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLS 640
Query: 503 GSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYFM 562
L + DF NL+ L L C +L +H SVG L LI L+ C++L++LP+
Sbjct: 641 YCHKLIQTPDFRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQ 700
Query: 563 LKSLEVLNLTKCIQLEEIPDLS-VASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNH 622
+ LEVL+L C L+ P++ ++LK+L L+ + +R + S L+ L L ++
Sbjct: 701 SECLEVLDLNYCFNLKMFPEVERNMTHLKKLDLTS-TGIRELPASIEH-LSSLENLQMHS 760
Query: 623 CDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCEN 682
C+ + LP ++ N I E + SL + N +L L +
Sbjct: 761 CNQLVSLPS-------SIWRFRNLKIS---ECEKLGSLPEIHGNSNCTRELILKLVSIKE 820
Query: 683 LEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALNL 742
L T ++L L + +C + + S L L L +C+ L+ LP A+N
Sbjct: 821 LP--TSIGNLTSLNFLEICNCKTISSLSSSIWGLTSLTTLKLLDCRKLKNLPGIPNAINH 880
Query: 743 KL---LNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYL-KLKSLDSLNLDGCI 802
L L + T++E LD L +++ +C + LP + LK L L + C
Sbjct: 881 LSGHGLQLLLTLEQPTIYE---RLDLLRIIDMSWCSCISSLPHNIWMLKFLRILCISYCS 940
Query: 803 KLEQLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLK 862
+LE LPE +++ L +++ D TGI LP S+ L L+ LS + A+ ++
Sbjct: 941 RLEYLPENLGHLEHLEELLADGTGILRLPSSVARLNKLEVLSFR---KKFAIGPKVQYSS 1000
Query: 863 SLYELCLRECSKLDMFPQRSSLNYSQESLCSKLTILN----LENCNISNVDFLETLSNVS 915
S+ L L LN S C+ +N LE +I+ LE L +
Sbjct: 1001 SMLNLPDDVFGSLGSLGSVVKLNLSGNGFCNLPETMNQLFCLEYLDITFCQRLEALPELP 1037
BLAST of Lcy02g011350 vs. ExPASy Swiss-Prot
Match:
Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)
HSP 1 Score: 359.4 bits (921), Expect = 1.5e-97
Identity = 269/801 (33.58%), Postives = 433/801 (54.06%), Query Frame = 0
Query: 23 NRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMVGIYGM 82
++ ++D I+ IV ++ SK+ L ++ + VGID+ LE +E + + NGV+++GI+GM
Sbjct: 159 DKTDADCIRQIVDQISSKLCKISLSYL-QNIVGIDTHLEKIESLLEIGINGVRIMGIWGM 218
Query: 83 GGLGKTTLAKALYNKI------ANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN 142
GG+GKTT+A+A+++ + + +F+G FL +++E + G+ LQ LL +L+
Sbjct: 219 GGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKENKR---GMHSLQNALLSELLREK 278
Query: 143 LEVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQ-LEKLVGGRDWFGRGSKIIVTTRNKQ 202
NN + G + RL SKKVLIVLDD+D D LE L G DWFG GS+II+TTR+K
Sbjct: 279 ANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKH 338
Query: 203 LLSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVL 262
L+ + D ++++ L ++++LF HAF + P+ N+ LS +Y KGLPLAL V
Sbjct: 339 LIEKN--DIIYEVTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVW 398
Query: 263 GSSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDR 322
GS L N ++W+S + +N+ I D L+IS++GLE K +++FLDI+CF GE+ D
Sbjct: 399 GSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDY 458
Query: 323 IKDMLEACDLNPNYGITILMDLSLITI-EDNRLKMHDLIRQMGQTIVCRESSEPEKKSRL 382
I +LE+C + YG+ IL+D SL+ I E N+++MHDLI+ MG+ IV +P ++SRL
Sbjct: 459 ILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIV-NFQKDPGERSRL 518
Query: 383 WVAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIKFSK 442
W+AK++ ++ N+GT A++AI + + +A K MK LR+ + +
Sbjct: 519 WLAKEVEEVMSNNTGTMAMEAIWVS----SYSSTLRFSNQAVKNMKRLRVFNMGR-SSTH 578
Query: 443 NIFECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHV 502
+ LPN L+ C + + SFP S++ LV L ++H+ + L ++ L+ +
Sbjct: 579 YAIDYLPNNLRCF-VCTNYPWESFP-STFELKMLVHLQLRHNSLRHLWTETKHLPSLRRI 638
Query: 503 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPS 562
D S S L DF+ NL+ + L C +L+ +H S+G SK+I L C +LK+ P
Sbjct: 639 DLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFPC 698
Query: 563 YFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDL 622
++SLE L L C LE++P++ E+++ + GS R L +
Sbjct: 699 -VNVESLEYLGLRSCDSLEKLPEI-YGRMKPEIQIH-------MQGSGIRELPSSI---F 758
Query: 623 NHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNL-KLEYLKVLNLCG 682
+ +V +L + M +L PS++ +L+ L L++ G
Sbjct: 759 QYKTHVTKLLLWNMK-----------------------NLVALPSSICRLKSLVSLSVSG 818
Query: 683 CENLEEI-TDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCK---NLEMLPS 742
C LE + + NL + + +D + LR R L+KL+ K + E P
Sbjct: 819 CSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIR-LNKLIILMFRGFKDGVHFEFPPV 878
Query: 743 FSAALNLKLLNLSKCINL--RTVHESIGSLDKLVTLELKFCYNLEKLPTYL-KLKSLDSL 802
+L+ LNLS C NL + E IGSL L L+L N E LP+ + +L +L SL
Sbjct: 879 AEGLHSLEYLNLSYC-NLIDGGLPEEIGSLSSLKKLDLS-RNNFEHLPSSIAQLGALQSL 907
Query: 803 NLDGCIKLEQLPEFDKNMKSL 808
+L C +L QLPE + L
Sbjct: 939 DLKDCQRLTQLPELPPELNEL 907
BLAST of Lcy02g011350 vs. ExPASy TrEMBL
Match:
A0A0A0KNK0 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G494390 PE=4 SV=1)
HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 621/1045 (59.43%), Postives = 746/1045 (71.39%), Query Frame = 0
Query: 18 YDVLNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSHVVSNGVQM 77
+D+ + E+DLI D+VKEVLS +N TQLLHVA HPVGIDSQL A+ EL SH V +GV M
Sbjct: 163 WDLATRKNEADLIHDLVKEVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNM 222
Query: 78 VGIYGMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN 137
VGI+GMGG+GKTTLAKALYNKIA +FE FLSNVRE +QF LVQLQE LL ILK N
Sbjct: 223 VGIHGMGGIGKTTLAKALYNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDN 282
Query: 138 L-EVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQ 197
+V NV +G IIRDRLCSKKVLI+LDDVDK +QL+ LVG RDWFGRGSKII TTR++
Sbjct: 283 AWKVGNVHKGKNIIRDRLCSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRH 342
Query: 198 LLSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVL 257
LL +H FD ++ IQ LD K+LELFS HAFK++HPSSNYVDLSK A SYCKGLPLALV+L
Sbjct: 343 LLENHSFDIVYPIQLLDPKKSLELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVIL 402
Query: 258 GSSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDR 317
GS L R++ W+S L+E ENSL ++ + QI F L ++VK+IFLDISCFFVGED++
Sbjct: 403 GSLLHKRERKIWKSKLHELENSLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINY 462
Query: 318 IKDMLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLW 377
KD+L+ACDLNP+YGI ILMDLSL+T+ED +++MHDLI+QMGQTIV ES EP K+SRLW
Sbjct: 463 SKDVLKACDLNPDYGIIILMDLSLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLW 522
Query: 378 VAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIK-FSK 437
A+ I +LKE SGT AVKAIKLDL + K+V + +AF+ MKNLRLLI++ + F K
Sbjct: 523 EAEGAIKILKEKSGTKAVKAIKLDLHYKPWLKIV--EAEAFRNMKNLRLLILQRVAYFPK 582
Query: 438 NIFECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHV 497
NIFE LPN LKWIE S IS V +LVGL +K ++ EN + +KHV
Sbjct: 583 NIFEYLPNSLKWIEWSTFYVNQSSSISFSVKGRLVGLVMKGVVNKQPRIAFENCKTMKHV 642
Query: 498 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLP- 557
D S L+E +FSA LNL++LYLR C SLK+IHESV SLSKL+TLD +GC NL+K P
Sbjct: 643 DLSYCGTLKETPNFSATLNLEKLYLRGCTSLKVIHESVASLSKLVTLDLEGCDNLEKFPS 702
Query: 558 SYFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLD 617
SY MLKSLEVLNL++C ++EEIPDLS +SNLKEL L EC LRIIH S RSL+KL+ LD
Sbjct: 703 SYLMLKSLEVLNLSRCRKIEEIPDLSASSNLKELYLRECDRLRIIHDSIGRSLDKLIILD 762
Query: 618 LNHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCG 677
L C N++RLP Y + LE L N L LE S KFPS+LK + LKVLNL
Sbjct: 763 LEGCKNLERLPIY--TNKLESLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRD 822
Query: 678 CENLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAA 737
C NLEEITDFS++SNLEIL+L C +LR+I
Sbjct: 823 CLNLEEITDFSMASNLEILDLNTCFSLRII------------------------------ 882
Query: 738 LNLKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIK 797
HESIGSLDKL+TL+L C+NLEKLP+ LKLKSLDSL+ C K
Sbjct: 883 -----------------HESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYK 942
Query: 798 LEQLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKS 857
LEQLPEFD+NMKSLR M L+ T I+ LP SIG+LIGL+ L+L C NL ALP+EIH LKS
Sbjct: 943 LEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKS 1002
Query: 858 LYELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSN 917
L EL LR CSKLDMFP RSSLN+SQES KLT+L+L+NCNISN DFLETLSNV +SL
Sbjct: 1003 LEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEK 1062
Query: 918 LNLSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYI 977
LNLSGN FSCLPSLQNFKSL+ LELR+CKFLQNI P H L +V+A G E L I PD I
Sbjct: 1063 LNLSGNTFSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHH-LARVNASGSELLAIRPDCI 1122
Query: 978 ADIIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVV 1037
AD++F QD ++ L + N+EIP Y N QTT SS+SV F H+ + N AL +CV+
Sbjct: 1123 ADMMFGKQDAEFSDSTKVLFITNNEIPKYCNKQTTRSSMSVRFRHNLDKN-IPALVLCVI 1154
Query: 1038 FKVDGDSC-EATAAIDCDVYVDNKL 1058
FK DGDSC EA I +V +D ++
Sbjct: 1183 FKADGDSCDEAEGFIHFEVSIDGEI 1154
BLAST of Lcy02g011350 vs. ExPASy TrEMBL
Match:
A0A0A0LWV1 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G334920 PE=4 SV=1)
HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 643/1105 (58.19%), Postives = 778/1105 (70.41%), Query Frame = 0
Query: 26 ESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSHVVS-NGVQMVGIYGMG 85
E+ LIQD+VK+V S + TQLL+VA HPV IDSQL+A+ EL SH VS NGV MVGI+GMG
Sbjct: 173 EAHLIQDLVKKV-SILKQTQLLNVAKHPVAIDSQLKAIEELASHGVSDNGVNMVGIHGMG 232
Query: 86 GLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILK-VNLEVNNV 145
G+GKTTLAKALYNKI +FE FLSNVRE S+QFNGLVQLQE LL I K NL+V+NV
Sbjct: 233 GIGKTTLAKALYNKITYQFEACCFLSNVRETSEQFNGLVQLQEKLLNEIFKDNNLKVDNV 292
Query: 146 DRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSHGF 205
D+G+ II+DRLCS+KVL+VLDDVDK DQL+ LVGGRDWFGRGSKIIVTTR++ LL ++ F
Sbjct: 293 DKGMNIIKDRLCSRKVLMVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLETYSF 352
Query: 206 DKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLCNR 265
DK+H IQ LD DK+LELF WHAFK+SHPS NY +L + YC GLPLALV+LGS LC R
Sbjct: 353 DKIHPIQLLDCDKSLELFCWHAFKQSHPSRNYSELPE-LVRYCNGLPLALVILGSLLCKR 412
Query: 266 DQSKWQSILYEFENSLTKDIKDILQISFNGLEDK--VKDIFLDISCFFVGEDVDRIKDML 325
DQ W+S L E +N I+ + QISF L + VK+IFLDI CFFVGEDV K++L
Sbjct: 413 DQIIWKSKLDELKNFPEPGIEAVFQISFKRLPENPPVKEIFLDICCFFVGEDVSYSKNVL 472
Query: 326 EACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAKDI 385
+ACD I ILMDLSL+T+ED +++MHDLIRQMGQ IV R+S +PEK+SRLWVAK+
Sbjct: 473 KACDPYLESRIIILMDLSLVTVEDGKIQMHDLIRQMGQMIVRRKSFKPEKRSRLWVAKEA 532
Query: 386 INMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIFEC 445
+ ML E SGT VKAIKLDL + ++ +AF+ M+NLRLLI++N K NIF+
Sbjct: 533 VKMLIEKSGTHKVKAIKLDLR---NNGSLIVEAEAFRNMENLRLLILQNAAKLPTNIFKY 592
Query: 446 LPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFSGS 505
LPN +KWIE S FPIS VN LVGL + + G I E+ + LKHVD S
Sbjct: 593 LPN-IKWIEYSSSSVRWYFPISFVVNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLSYW 652
Query: 506 FVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF-ML 565
+LEE DFSAALNL++LYL C LKMIH SV SLSKL+TLD +GC NL+KLPS F ML
Sbjct: 653 RLLEETPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCENLEKLPSSFLML 712
Query: 566 KSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGS-TSRSLNKLVTLDLNHC 625
KSLEVLNL+ CI+L+EIPDLS +SNLKEL L EC +LRIIH S R L+KLV LDL C
Sbjct: 713 KSLEVLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHDSAVGRFLDKLVILDLEGC 772
Query: 626 DNVKRLPCYVM-SKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCEN 685
++RLP Y+ SKS+E+ L + ++ D+YF KFPS+LK E LKVLNL C+N
Sbjct: 773 KILERLPRYISNSKSIEVMNLDSCR-KIEQLFDNYFE--KFPSHLKFESLKVLNLSYCQN 832
Query: 686 LEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALNL 745
L+EITDFSI+SNLEI +L
Sbjct: 833 LKEITDFSIASNLEIFDLRG---------------------------------------- 892
Query: 746 KLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLEQ 805
C +LRT+H+S+GSLD+L+ L+L FC+ LE+LP+ L+LKSLDSL+L C K+EQ
Sbjct: 893 -------CFSLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSLTNCYKIEQ 952
Query: 806 LPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYE 865
LPEFD+NMKSLR+M L T I++LP SI +LIGL+ L L CTNLI+LPSEIHLLKSL E
Sbjct: 953 LPEFDENMKSLREMNLKGTAIRKLPTSIRYLIGLENLILSYCTNLISLPSEIHLLKSLKE 1012
Query: 866 LCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLNL 925
L LRECS+LDM P SSLN+ Q SLCS LTIL+L+NCNISN DFLE LSN ++L LNL
Sbjct: 1013 LDLRECSRLDMLPSGSSLNFPQRSLCSNLTILDLQNCNISNSDFLENLSNFCTTLKELNL 1072
Query: 926 SGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYIADI 985
SGNKF CLPSL+NF SL+ LELR+CKFL+NI P HCL+++DA GCE LVISPDYIAD+
Sbjct: 1073 SGNKFCCLPSLKNFTSLRLLELRNCKFLRNIVKIP-HCLKRMDASGCELLVISPDYIADM 1132
Query: 986 IFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFKV 1045
+FR QD R+ R LI+ SEIP + NNQTTESS+S SF+H N+ AL VCVVFKV
Sbjct: 1133 MFRNQDLKLRNFKRELIVTYSEIPKFCNNQTTESSISFSFQH-NSDMIIPALVVCVVFKV 1192
Query: 1046 DGDSCEATAAIDCDVYVD-NKLSITLSEGFGWSKSEFMWIIRTP--TLRCLDMKALHKIT 1105
D DS A A I V D KL + E + SKSE M ++RTP L CL+ HKI
Sbjct: 1193 DADSFVAEAFIHFQVLFDGQKLMMPTMESWCGSKSEHMLLLRTPPSQLICLNENNRHKIE 1219
Query: 1106 VWFRVMMVYKSAIVSIRRHGVLVVD 1119
V F+V K A V IR GV VVD
Sbjct: 1253 VSFQVRNYNKKAKVIIRSLGVYVVD 1219
BLAST of Lcy02g011350 vs. ExPASy TrEMBL
Match:
A0A5D3C521 (TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001460 PE=4 SV=1)
HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 635/1108 (57.31%), Postives = 778/1108 (70.22%), Query Frame = 0
Query: 24 RKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSH-VVSNGVQMVGIYG 83
+ E+ LI D+V++V S + TQLL+VA HPVGIDSQL+A+ E SH V+ NGV MVGI+G
Sbjct: 144 KNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFASHGVLDNGVNMVGIHG 203
Query: 84 MGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILK-VNLEVN 143
MGG+GKTTLAKALYNKI EFE FLSNVREAS+QFNGLVQLQE LL I K NL+V+
Sbjct: 204 MGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQEKLLSEIFKDNNLKVD 263
Query: 144 NVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSH 203
NV +G+ I++DRLCS+KVLIVLDDVDK DQL+ LVGGRDWFGRGSKIIVTTR++ LL +
Sbjct: 264 NVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLERY 323
Query: 204 GFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLC 263
FDK+H IQ LD K+LELF WHAFK++HPS +Y+DLS+ YC GLPLALV+LGS LC
Sbjct: 324 SFDKIHPIQLLDYVKSLELFCWHAFKQNHPSRDYLDLSELVVRYCNGLPLALVILGSLLC 383
Query: 264 NRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDK--VKDIFLDISCFFVGEDVDRIKD 323
RDQ W+S L E +N I+ + QISF LE+ VK+IFLDI CFFVGEDV K+
Sbjct: 384 KRDQIIWKSKLDELKNFPEPGIEAVFQISFKRLEENPPVKEIFLDICCFFVGEDVSYSKN 443
Query: 324 MLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAK 383
+L+ACD I ILMDLSL+T+ED +++MHDLIRQMGQTIV +SS+PEK+SRLWVAK
Sbjct: 444 VLKACDPYLESRIIILMDLSLVTVEDGKIQMHDLIRQMGQTIVRCKSSKPEKRSRLWVAK 503
Query: 384 DIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIF 443
+ + ML E SGT VKAIKLDL + + ++ +AF+ M+NLRLLI++N KF NIF
Sbjct: 504 EAVKMLIEKSGTHKVKAIKLDLR---SNRPLIVEAEAFRNMENLRLLILQNAAKFPTNIF 563
Query: 444 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFS 503
+ LPN +KWIE S FPIS VN LVGL + + G I E+ + LKHVD S
Sbjct: 564 KYLPN-IKWIEYSSSNVQWYFPISFVVNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLS 623
Query: 504 GSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF- 563
+LEE DFS ALNL++LYLR C L+MIH S+ SLSKL+TLD +GC NL+KLPS F
Sbjct: 624 YWRLLEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFL 683
Query: 564 MLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNH 623
MLKSLEVLNL+ CI+L+EIPDLS +S+LKEL L EC NLRIIH S R L+KLV LD
Sbjct: 684 MLKSLEVLNLSGCIKLKEIPDLSASSSLKELHLRECYNLRIIHDSVGRFLDKLVILDFEG 743
Query: 624 CDNVKRLPCYV-MSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCE 683
C N++RLP Y+ S S+E+ L + ++ D+YF KFP++LK E LKVLNL C+
Sbjct: 744 CRNLERLPRYISKSGSIEVLNLDSCR-KIEQIFDNYFE--KFPNHLKYESLKVLNLSYCQ 803
Query: 684 NLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN 743
NL+ ITDFS +SNLEIL+L
Sbjct: 804 NLKGITDFSFASNLEILDLRG--------------------------------------- 863
Query: 744 LKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLE 803
C +LRT+HES+GSLDKL+ L+L C+ LE+LP+ L+LKSLDSL+L C KLE
Sbjct: 864 --------CFSLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSLTNCYKLE 923
Query: 804 QLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLY 863
QLPEFD+NMKSLR+M L T I++LP SI +LIGL+ L+L CTNLI+LPSEIHLLKSL
Sbjct: 924 QLPEFDENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEIHLLKSLK 983
Query: 864 ELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLN 923
EL L ECS+LDMFP SSLN+ Q+SL S LTIL+L+NCNISN DFLE LSN ++L LN
Sbjct: 984 ELDLHECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFCTTLKELN 1043
Query: 924 LSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYIAD 983
LSGNKF LPSLQNF SL+ LELR+CKFL+NI P HCL +VDA GCE VISPDYIAD
Sbjct: 1044 LSGNKFCSLPSLQNFTSLRHLELRNCKFLRNIVKIP-HCLTRVDASGCELFVISPDYIAD 1103
Query: 984 IIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFK 1043
++FR QD R+ R LI+ S+IP + NNQTTESS S SF+ N+ AL VCVVFK
Sbjct: 1104 MMFRNQDLELRNFKRELIVAYSKIPKFCNNQTTESSTSFSFQ-QNSDTIIPALVVCVVFK 1163
Query: 1044 VDGDSCEATAAIDCDVYVDNK-LSITLSEGFGWSKSEFMWIIRTP--TLRCLDMKALHKI 1103
VD DSC+A I V +D + L + EG+ SKSE M ++RTP L L+ HKI
Sbjct: 1164 VDEDSCDAEGFIRFQVLIDGQMLMMPTMEGWCGSKSEHMLLLRTPPSQLIYLNENNRHKI 1194
Query: 1104 TVWFRVMMVYKSAIVSIRRHGVLVVDAI 1121
V F+V K A V IR GV VV+ +
Sbjct: 1224 EVSFQVWNYKKKAKVVIRSLGVYVVNEL 1194
BLAST of Lcy02g011350 vs. ExPASy TrEMBL
Match:
A0A5A7TTC7 (TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G001060 PE=4 SV=1)
HSP 1 Score: 1057.4 bits (2733), Expect = 4.4e-305
Identity = 609/1039 (58.61%), Postives = 741/1039 (71.32%), Query Frame = 0
Query: 24 RKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSH-VVSNGVQMVGIYG 83
+ E+ LI D+V++V S + TQLL+VA HPVGIDSQL+A+ E SH V+ NGV MVGI+G
Sbjct: 170 KNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFASHGVLDNGVNMVGIHG 229
Query: 84 MGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILK-VNLEVN 143
MGG+GKTTLAKALYNKI EFE FLSNVREAS+QFNGLVQLQE LL I K NL+V+
Sbjct: 230 MGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQEKLLSEIFKDNNLKVD 289
Query: 144 NVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSH 203
NV +G+ I++DRLCS+KVLIVLDDVDK DQL+ LVGGRDWFGRGSKIIVTTR++ LL +
Sbjct: 290 NVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLERY 349
Query: 204 GFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLC 263
FDK+H IQ LD K+LELF WHAFK++HPS +Y+DLS+ YC GLPLALV+LGS LC
Sbjct: 350 SFDKIHPIQLLDYVKSLELFCWHAFKQNHPSRDYLDLSELVVRYCNGLPLALVILGSLLC 409
Query: 264 NRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDK--VKDIFLDISCFFVGEDVDRIKD 323
RDQ W+S L E +N I+ + QISF LE+ VK+IFLDI CFFVGEDV K+
Sbjct: 410 KRDQKIWKSKLDELKNFPEPGIEAVFQISFKRLEENPPVKEIFLDICCFFVGEDVSYSKN 469
Query: 324 MLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAK 383
+L+ACD I ILMDLSL+T+ED +++MHDLIRQMGQTIV +SS+PEK+SRLWVAK
Sbjct: 470 VLKACDPYLESRIIILMDLSLVTVEDGKIQMHDLIRQMGQTIVRCKSSKPEKRSRLWVAK 529
Query: 384 DIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIF 443
+ + ML E SGT VKAIKLDL + + ++ +AF+ M+NLRLLI++N KF NIF
Sbjct: 530 EAVKMLIEKSGTHKVKAIKLDLR---SNRPLIVEAEAFRNMENLRLLILQNAAKFPTNIF 589
Query: 444 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFS 503
+ LPN +KWIE S FPIS VN LVGL + + G I E+ + LKHVD S
Sbjct: 590 KYLPN-IKWIEYSSSNVQWYFPISFVVNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLS 649
Query: 504 GSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF- 563
+LEE DFS ALNL++LYLR C L+MIH S+ SLSKL+TLD +GC NL+KLPS F
Sbjct: 650 YWRLLEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFL 709
Query: 564 MLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNH 623
MLKSLEVLNL+ CI+L+EIPDLS +S+LKEL L EC NLRIIH S R L+KLV LD
Sbjct: 710 MLKSLEVLNLSGCIKLKEIPDLSASSSLKELHLRECYNLRIIHDSVGRFLDKLVILDFEG 769
Query: 624 CDNVKRLPCYV-MSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCE 683
C N++RLP Y+ S S+E+ L + ++ D+YF KFPS+LK E LKVLNL C+
Sbjct: 770 CRNLERLPRYISKSGSIEVLNLDSCR-KIEQIFDNYFE--KFPSHLKYESLKVLNLSYCQ 829
Query: 684 NLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN 743
NL+ ITDFS +SNLEIL+L
Sbjct: 830 NLKGITDFSFASNLEILDLRG--------------------------------------- 889
Query: 744 LKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLE 803
C +LRT+HES+GSLDKL+ L+L C+ LE+LP+ L+LKSLDSL+L C KLE
Sbjct: 890 --------CFSLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSLTNCYKLE 949
Query: 804 QLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLY 863
QLPEFD+NMKSLR+M L T I++LP SI +LIGL+ L+L CTNLI+LPSEIHLLKSL
Sbjct: 950 QLPEFDENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEIHLLKSLK 1009
Query: 864 ELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLN 923
EL L ECS+LDMFP SSLN+ Q+SL S LTIL+L+NCNISN DFLE LSN ++L LN
Sbjct: 1010 ELDLHECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFCTTLKELN 1069
Query: 924 LSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYIAD 983
LSGNKF LPSLQNF SL+ LELR+CKFL+NI P HCL +VDA GCE VISPDYIAD
Sbjct: 1070 LSGNKFCSLPSLQNFSSLRHLELRNCKFLRNIVKIP-HCLTRVDASGCELFVISPDYIAD 1129
Query: 984 IIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFK 1043
I+FR QD R+ R LI+ S+IP + NNQTTESS S SF+ N+ AL VCVVFK
Sbjct: 1130 IMFRNQDLELRNFKRELIVAYSKIPKFCNNQTTESSTSFSFQ-QNSDTIIPALVVCVVFK 1151
Query: 1044 VDGDSCEATAAIDCDVYVD 1055
VD DSC+A I V +D
Sbjct: 1190 VDEDSCDAEGFIRFQVLID 1151
BLAST of Lcy02g011350 vs. ExPASy TrEMBL
Match:
A0A1S3B9C2 (TMV resistance protein N OS=Cucumis melo OX=3656 GN=LOC103487452 PE=4 SV=1)
HSP 1 Score: 1057.4 bits (2733), Expect = 4.4e-305
Identity = 609/1039 (58.61%), Postives = 741/1039 (71.32%), Query Frame = 0
Query: 24 RKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSH-VVSNGVQMVGIYG 83
+ E+ LI D+V++V S + TQLL+VA HPVGIDSQL+A+ E SH V+ NGV MVGI+G
Sbjct: 170 KNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFASHGVLDNGVNMVGIHG 229
Query: 84 MGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILK-VNLEVN 143
MGG+GKTTLAKALYNKI EFE FLSNVREAS+QFNGLVQLQE LL I K NL+V+
Sbjct: 230 MGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQEKLLSEIFKDNNLKVD 289
Query: 144 NVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSH 203
NV +G+ I++DRLCS+KVLIVLDDVDK DQL+ LVGGRDWFGRGSKIIVTTR++ LL +
Sbjct: 290 NVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLERY 349
Query: 204 GFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLC 263
FDK+H IQ LD K+LELF WHAFK++HPS +Y+DLS+ YC GLPLALV+LGS LC
Sbjct: 350 SFDKIHPIQLLDYVKSLELFCWHAFKQNHPSRDYLDLSELVVRYCNGLPLALVILGSLLC 409
Query: 264 NRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDK--VKDIFLDISCFFVGEDVDRIKD 323
RDQ W+S L E +N I+ + QISF LE+ VK+IFLDI CFFVGEDV K+
Sbjct: 410 KRDQKIWKSKLDELKNFPEPGIEAVFQISFKRLEENPPVKEIFLDICCFFVGEDVSYSKN 469
Query: 324 MLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAK 383
+L+ACD I ILMDLSL+T+ED +++MHDLIRQMGQTIV +SS+PEK+SRLWVAK
Sbjct: 470 VLKACDPYLESRIIILMDLSLVTVEDGKIQMHDLIRQMGQTIVRCKSSKPEKRSRLWVAK 529
Query: 384 DIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIF 443
+ + ML E SGT VKAIKLDL + + ++ +AF+ M+NLRLLI++N KF NIF
Sbjct: 530 EAVKMLIEKSGTHKVKAIKLDLR---SNRPLIVEAEAFRNMENLRLLILQNAAKFPTNIF 589
Query: 444 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFS 503
+ LPN +KWIE S FPIS VN LVGL + + G I E+ + LKHVD S
Sbjct: 590 KYLPN-IKWIEYSSSNVQWYFPISFVVNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLS 649
Query: 504 GSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF- 563
+LEE DFS ALNL++LYLR C L+MIH S+ SLSKL+TLD +GC NL+KLPS F
Sbjct: 650 YWRLLEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFL 709
Query: 564 MLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNH 623
MLKSLEVLNL+ CI+L+EIPDLS +S+LKEL L EC NLRIIH S R L+KLV LD
Sbjct: 710 MLKSLEVLNLSGCIKLKEIPDLSASSSLKELHLRECYNLRIIHDSVGRFLDKLVILDFEG 769
Query: 624 CDNVKRLPCYV-MSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCE 683
C N++RLP Y+ S S+E+ L + ++ D+YF KFPS+LK E LKVLNL C+
Sbjct: 770 CRNLERLPRYISKSGSIEVLNLDSCR-KIEQIFDNYFE--KFPSHLKYESLKVLNLSYCQ 829
Query: 684 NLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN 743
NL+ ITDFS +SNLEIL+L
Sbjct: 830 NLKGITDFSFASNLEILDLRG--------------------------------------- 889
Query: 744 LKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLE 803
C +LRT+HES+GSLDKL+ L+L C+ LE+LP+ L+LKSLDSL+L C KLE
Sbjct: 890 --------CFSLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSLTNCYKLE 949
Query: 804 QLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLY 863
QLPEFD+NMKSLR+M L T I++LP SI +LIGL+ L+L CTNLI+LPSEIHLLKSL
Sbjct: 950 QLPEFDENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEIHLLKSLK 1009
Query: 864 ELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLN 923
EL L ECS+LDMFP SSLN+ Q+SL S LTIL+L+NCNISN DFLE LSN ++L LN
Sbjct: 1010 ELDLHECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFCTTLKELN 1069
Query: 924 LSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYIAD 983
LSGNKF LPSLQNF SL+ LELR+CKFL+NI P HCL +VDA GCE VISPDYIAD
Sbjct: 1070 LSGNKFCSLPSLQNFSSLRHLELRNCKFLRNIVKIP-HCLTRVDASGCELFVISPDYIAD 1129
Query: 984 IIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFK 1043
I+FR QD R+ R LI+ S+IP + NNQTTESS S SF+ N+ AL VCVVFK
Sbjct: 1130 IMFRNQDLELRNFKRELIVAYSKIPKFCNNQTTESSTSFSFQ-QNSDTIIPALVVCVVFK 1151
Query: 1044 VDGDSCEATAAIDCDVYVD 1055
VD DSC+A I V +D
Sbjct: 1190 VDEDSCDAEGFIRFQVLID 1151
BLAST of Lcy02g011350 vs. NCBI nr
Match:
XP_031741747.1 (TMV resistance protein N [Cucumis sativus])
HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 636/1119 (56.84%), Postives = 768/1119 (68.63%), Query Frame = 0
Query: 18 YDVLNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSHVVSNGVQM 77
+D+ + E+DLI D+VKEVLS +N TQLLHVA HPVGIDSQL A+ EL SH V +GV M
Sbjct: 163 WDLATRKNEADLIHDLVKEVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNM 222
Query: 78 VGIYGMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN 137
VGI+GMGG+GKTTLAKALYNKIA +FE FLSNVRE +QF LVQLQE LL ILK N
Sbjct: 223 VGIHGMGGIGKTTLAKALYNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDN 282
Query: 138 L-EVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQ 197
+V NV +G IIRDRLCSKKVLI+LDDVDK +QL+ LVG RDWFGRGSKII TTR++
Sbjct: 283 AWKVGNVHKGKNIIRDRLCSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRH 342
Query: 198 LLSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVL 257
LL +H FD ++ IQ LD K+LELFS HAFK++HPSSNYVDLSK A SYCKGLPLALV+L
Sbjct: 343 LLENHSFDIVYPIQLLDPKKSLELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVIL 402
Query: 258 GSSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDR 317
GS L R++ W+S L+E ENSL ++ + QI F L ++VK+IFLDISCFFVGED++
Sbjct: 403 GSLLHKRERKIWKSKLHELENSLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINY 462
Query: 318 IKDMLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLW 377
KD+L+ACDLNP+YGI ILMDLSL+T+ED +++MHDLI+QMGQTIV ES EP K+SRLW
Sbjct: 463 SKDVLKACDLNPDYGIIILMDLSLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLW 522
Query: 378 VAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIK-FSK 437
A+ I +LKE SGT AVKAIKLDL + K+V + +AF+ MKNLRLLI++ + F K
Sbjct: 523 EAEGAIKILKEKSGTKAVKAIKLDLHYKPWLKIV--EAEAFRNMKNLRLLILQRVAYFPK 582
Query: 438 NIFECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHV 497
NIFE LPN LKWIE S IS V +LVGL +K ++ EN + +KHV
Sbjct: 583 NIFEYLPNSLKWIEWSTFYVNQSSSISFSVKGRLVGLVMKGVVNKQPRIAFENCKTMKHV 642
Query: 498 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLP- 557
D S L+E +FSA LNL++LYLR C SLK+IHESV SLSKL+TLD +GC NL+K P
Sbjct: 643 DLSYCGTLKETPNFSATLNLEKLYLRGCTSLKVIHESVASLSKLVTLDLEGCDNLEKFPS 702
Query: 558 SYFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLD 617
SY MLKSLEVLNL++C ++EEIPDLS +SNLKEL L EC LRIIH S RSL+KL+ LD
Sbjct: 703 SYLMLKSLEVLNLSRCRKIEEIPDLSASSNLKELYLRECDRLRIIHDSIGRSLDKLIILD 762
Query: 618 LNHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCG 677
L C N++RLP Y TN KLE L++LNL
Sbjct: 763 LEGCKNLERLPIY-----------TN----------------------KLESLELLNLAS 822
Query: 678 CENLE----EITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPS 737
C LE +ITDFS++SNLEIL+L C +LR+I
Sbjct: 823 CLKLETFFDKITDFSMASNLEILDLNTCFSLRII-------------------------- 882
Query: 738 FSAALNLKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLD 797
HESIGSLDKL+TL+L C+NLEKLP+ LKLKSLDSL+
Sbjct: 883 ---------------------HESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFT 942
Query: 798 GCIKLEQLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIH 857
C KLEQLPEFD+NMKSLR M L+ T I+ LP SIG+LIGL+ L+L C NL ALP+EIH
Sbjct: 943 NCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIH 1002
Query: 858 LLKSLYELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSS 917
LKSL EL LR CSKLDMFP RSSLN+SQES KLT+L+L+NCNISN DFLETLSNV +
Sbjct: 1003 WLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCT 1062
Query: 918 SLSNLNLSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVIS 977
SL LNLSGN FSCLPSLQNFKSL+ LELR+CKFLQNI P H L +V+A G E L I
Sbjct: 1063 SLEKLNLSGNTFSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHH-LARVNASGSELLAIR 1122
Query: 978 PDYIADIIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALA 1037
PD IAD++F QD ++ L + N+EIP Y N QTT SS+SV F H+ + N AL
Sbjct: 1123 PDCIADMMFGKQDAEFSDSTKVLFITNNEIPKYCNKQTTRSSMSVRFRHNLDKN-IPALV 1182
Query: 1038 VCVVFKVDGDSC-EATAAIDCDVYVDNKLSITLSEGFGW-SKSEFMWIIRT-PT-LRCLD 1097
+CV+FK DGDSC EA I +V +D ++ + + G W SKSE M ++RT PT LR L
Sbjct: 1183 LCVIFKADGDSCDEAEGFIHFEVSIDGEIIMASTVGCCWSSKSEHMLLLRTSPTKLRYLH 1197
Query: 1098 MKALHKITVWFR------VMMVYKSAIVSIRRHGVLVVD 1119
H I V F V +KSA V +R GV +VD
Sbjct: 1243 ANDRHHIKVLFPNTTSKFVSKRFKSANVIMRTQGVYMVD 1197
BLAST of Lcy02g011350 vs. NCBI nr
Match:
TYK06440.1 (TMV resistance protein N [Cucumis melo var. makuwa])
HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 635/1108 (57.31%), Postives = 778/1108 (70.22%), Query Frame = 0
Query: 24 RKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSH-VVSNGVQMVGIYG 83
+ E+ LI D+V++V S + TQLL+VA HPVGIDSQL+A+ E SH V+ NGV MVGI+G
Sbjct: 144 KNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFASHGVLDNGVNMVGIHG 203
Query: 84 MGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILK-VNLEVN 143
MGG+GKTTLAKALYNKI EFE FLSNVREAS+QFNGLVQLQE LL I K NL+V+
Sbjct: 204 MGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQEKLLSEIFKDNNLKVD 263
Query: 144 NVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSH 203
NV +G+ I++DRLCS+KVLIVLDDVDK DQL+ LVGGRDWFGRGSKIIVTTR++ LL +
Sbjct: 264 NVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLERY 323
Query: 204 GFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLC 263
FDK+H IQ LD K+LELF WHAFK++HPS +Y+DLS+ YC GLPLALV+LGS LC
Sbjct: 324 SFDKIHPIQLLDYVKSLELFCWHAFKQNHPSRDYLDLSELVVRYCNGLPLALVILGSLLC 383
Query: 264 NRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDK--VKDIFLDISCFFVGEDVDRIKD 323
RDQ W+S L E +N I+ + QISF LE+ VK+IFLDI CFFVGEDV K+
Sbjct: 384 KRDQIIWKSKLDELKNFPEPGIEAVFQISFKRLEENPPVKEIFLDICCFFVGEDVSYSKN 443
Query: 324 MLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAK 383
+L+ACD I ILMDLSL+T+ED +++MHDLIRQMGQTIV +SS+PEK+SRLWVAK
Sbjct: 444 VLKACDPYLESRIIILMDLSLVTVEDGKIQMHDLIRQMGQTIVRCKSSKPEKRSRLWVAK 503
Query: 384 DIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIF 443
+ + ML E SGT VKAIKLDL + + ++ +AF+ M+NLRLLI++N KF NIF
Sbjct: 504 EAVKMLIEKSGTHKVKAIKLDLR---SNRPLIVEAEAFRNMENLRLLILQNAAKFPTNIF 563
Query: 444 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFS 503
+ LPN +KWIE S FPIS VN LVGL + + G I E+ + LKHVD S
Sbjct: 564 KYLPN-IKWIEYSSSNVQWYFPISFVVNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLS 623
Query: 504 GSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF- 563
+LEE DFS ALNL++LYLR C L+MIH S+ SLSKL+TLD +GC NL+KLPS F
Sbjct: 624 YWRLLEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFL 683
Query: 564 MLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNH 623
MLKSLEVLNL+ CI+L+EIPDLS +S+LKEL L EC NLRIIH S R L+KLV LD
Sbjct: 684 MLKSLEVLNLSGCIKLKEIPDLSASSSLKELHLRECYNLRIIHDSVGRFLDKLVILDFEG 743
Query: 624 CDNVKRLPCYV-MSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCE 683
C N++RLP Y+ S S+E+ L + ++ D+YF KFP++LK E LKVLNL C+
Sbjct: 744 CRNLERLPRYISKSGSIEVLNLDSCR-KIEQIFDNYFE--KFPNHLKYESLKVLNLSYCQ 803
Query: 684 NLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN 743
NL+ ITDFS +SNLEIL+L
Sbjct: 804 NLKGITDFSFASNLEILDLRG--------------------------------------- 863
Query: 744 LKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLE 803
C +LRT+HES+GSLDKL+ L+L C+ LE+LP+ L+LKSLDSL+L C KLE
Sbjct: 864 --------CFSLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSLTNCYKLE 923
Query: 804 QLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLY 863
QLPEFD+NMKSLR+M L T I++LP SI +LIGL+ L+L CTNLI+LPSEIHLLKSL
Sbjct: 924 QLPEFDENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEIHLLKSLK 983
Query: 864 ELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLN 923
EL L ECS+LDMFP SSLN+ Q+SL S LTIL+L+NCNISN DFLE LSN ++L LN
Sbjct: 984 ELDLHECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFCTTLKELN 1043
Query: 924 LSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYIAD 983
LSGNKF LPSLQNF SL+ LELR+CKFL+NI P HCL +VDA GCE VISPDYIAD
Sbjct: 1044 LSGNKFCSLPSLQNFTSLRHLELRNCKFLRNIVKIP-HCLTRVDASGCELFVISPDYIAD 1103
Query: 984 IIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFK 1043
++FR QD R+ R LI+ S+IP + NNQTTESS S SF+ N+ AL VCVVFK
Sbjct: 1104 MMFRNQDLELRNFKRELIVAYSKIPKFCNNQTTESSTSFSFQ-QNSDTIIPALVVCVVFK 1163
Query: 1044 VDGDSCEATAAIDCDVYVDNK-LSITLSEGFGWSKSEFMWIIRTP--TLRCLDMKALHKI 1103
VD DSC+A I V +D + L + EG+ SKSE M ++RTP L L+ HKI
Sbjct: 1164 VDEDSCDAEGFIRFQVLIDGQMLMMPTMEGWCGSKSEHMLLLRTPPSQLIYLNENNRHKI 1194
Query: 1104 TVWFRVMMVYKSAIVSIRRHGVLVVDAI 1121
V F+V K A V IR GV VV+ +
Sbjct: 1224 EVSFQVWNYKKKAKVVIRSLGVYVVNEL 1194
BLAST of Lcy02g011350 vs. NCBI nr
Match:
KGN65609.2 (hypothetical protein Csa_019785 [Cucumis sativus])
HSP 1 Score: 1067.4 bits (2759), Expect = 8.7e-308
Identity = 629/1081 (58.19%), Postives = 759/1081 (70.21%), Query Frame = 0
Query: 50 AMHPVGIDSQLEAM-ELVSHVVS-NGVQMVGIYGMGGLGKTTLAKALYNKIANEFEGHSF 109
A HPV IDSQL+A+ EL SH VS NGV MVGI+GMGG+GKTTLAKALYNKI +FE F
Sbjct: 162 AKHPVAIDSQLKAIEELASHGVSDNGVNMVGIHGMGGIGKTTLAKALYNKITYQFEACCF 221
Query: 110 LSNVREASKQFNGLVQLQENLLYAILK-VNLEVNNVDRGITIIRDRLCSKKVLIVLDDVD 169
LSNVRE S+QFNGLVQLQE LL I K NL+V+NVD+G+ II+DRLCS+KVL+VLDDVD
Sbjct: 222 LSNVRETSEQFNGLVQLQEKLLNEIFKDNNLKVDNVDKGMNIIKDRLCSRKVLMVLDDVD 281
Query: 170 KLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSHGFDKMHDIQGLDQDKALELFSWHAFK 229
K DQL+ LVGGRDWFGRGSKIIVTTR++ LL ++ FDK+H IQ LD DK+LELF WHAFK
Sbjct: 282 KDDQLDALVGGRDWFGRGSKIIVTTRDRHLLETYSFDKIHPIQLLDCDKSLELFCWHAFK 341
Query: 230 ESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLCNRDQSKWQSILYEFENSLTKDIKDIL 289
+SHPS NY +L + YC GLPLALV+LGS LC RDQ W+S L E +N I+ +
Sbjct: 342 QSHPSRNYSELPE-LVRYCNGLPLALVILGSLLCKRDQIIWKSKLDELKNFPEPGIEAVF 401
Query: 290 QISFNGLEDK--VKDIFLDISCFFVGEDVDRIKDMLEACDLNPNYGITILMDLSLITIED 349
QISF L + VK+IFLDI CFFVGEDV K++L+ACD I ILMDLSL+T+ED
Sbjct: 402 QISFKRLPENPPVKEIFLDICCFFVGEDVSYSKNVLKACDPYLESRIIILMDLSLVTVED 461
Query: 350 NRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAKDIINMLKENSGTDAVKAIKLDLPFLV 409
+++MHDLIRQMGQ IV R+S +PEK+SRLWVAK+ + ML E SGT VKAIKLDL
Sbjct: 462 GKIQMHDLIRQMGQMIVRRKSFKPEKRSRLWVAKEAVKMLIEKSGTHKVKAIKLDLR--- 521
Query: 410 QEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIFECLPNKLKWIECCRSIGFPSFPISSY 469
+ ++ +AF+ M+NLRLLI++N K NIF+ LPN +KWIE S FPIS
Sbjct: 522 NNGSLIVEAEAFRNMENLRLLILQNAAKLPTNIFKYLPN-IKWIEYSSSSVRWYFPISFV 581
Query: 470 VNDQLVGLDVKHSFVERLGKILENSRRLKHVDFSGSFVLEEILDFSAALNLKELYLRDCG 529
VN LVGL + + G I E+ + LKHVD S +LEE DFSAALNL++LYL C
Sbjct: 582 VNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLSYWRLLEETPDFSAALNLEKLYLLSCK 641
Query: 530 SLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF-MLKSLEVLNLTKCIQLEEIPDLSVAS 589
LKMIH SV SLSKL+TLD +GC NL+KLPS F MLKSLEVLNL+ CI+L+EIPDLS +S
Sbjct: 642 RLKMIHGSVASLSKLVTLDLEGCENLEKLPSSFLMLKSLEVLNLSGCIKLKEIPDLSASS 701
Query: 590 NLKELRLSECSNLRIIHGS-TSRSLNKLVTLDLNHCDNVKRLPCYVM-SKSLELFILTNF 649
NLKEL L EC +LRIIH S R L+KLV LDL C ++RLP Y+ SKS+E+ L +
Sbjct: 702 NLKELHLRECYHLRIIHDSAVGRFLDKLVILDLEGCKILERLPRYISNSKSIEVMNLDSC 761
Query: 650 MIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCENLEEITDFSISSNLEILNLTDCINL 709
++ D+YF KFPS+LK E LKVLNL C+NL+EITDFSI+SNLEI +L
Sbjct: 762 R-KIEQLFDNYFE--KFPSHLKFESLKVLNLSYCQNLKEITDFSIASNLEIFDLRG---- 821
Query: 710 RMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALNLKLLNLSKCINLRTVHESIGSLDKL 769
C +LRT+H+S+GSLD+L
Sbjct: 822 -------------------------------------------CFSLRTIHKSVGSLDQL 881
Query: 770 VTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLEQLPEFDKNMKSLRKMMLDQTGIKEL 829
+ L+L FC+ LE+LP+ L+LKSLDSL+L C K+EQLPEFD+NMKSLR+M L T I++L
Sbjct: 882 IALKLDFCHQLEELPSCLRLKSLDSLSLTNCYKIEQLPEFDENMKSLREMNLKGTAIRKL 941
Query: 830 PPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYELCLRECSKLDMFPQRSSLNYSQES 889
P SI +LIGL+ L L CTNLI+LPSEIHLLKSL EL LRECS+LDM P SSLN+ Q S
Sbjct: 942 PTSIRYLIGLENLILSYCTNLISLPSEIHLLKSLKELDLRECSRLDMLPSGSSLNFPQRS 1001
Query: 890 LCSKLTILNLENCNISNVDFLETLSNVSSSLSNLNLSGNKFSCLPSLQNFKSLKSLELRH 949
LCS LTIL+L+NCNISN DFLE LSN ++L LNLSGNKF CLPSL+NF SL+ LELR+
Sbjct: 1002 LCSNLTILDLQNCNISNSDFLENLSNFCTTLKELNLSGNKFCCLPSLKNFTSLRLLELRN 1061
Query: 950 CKFLQNIANFPTHCLEKVDARGCESLVISPDYIADIIFRPQDNLERHRSRRLILMNSEIP 1009
CKFL+NI P HCL+++DA GCE LVISPDYIAD++FR QD R+ R LI+ SEIP
Sbjct: 1062 CKFLRNIVKIP-HCLKRMDASGCELLVISPDYIADMMFRNQDLKLRNFKRELIVTYSEIP 1121
Query: 1010 NYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFKVDGDSCEATAAIDCDVYVD-NKLSI 1069
+ NNQTTESS+S SF+H N+ AL VCVVFKVD DS A A I V D KL +
Sbjct: 1122 KFCNNQTTESSISFSFQH-NSDMIIPALVVCVVFKVDADSFVAEAFIHFQVLFDGQKLMM 1181
Query: 1070 TLSEGFGWSKSEFMWIIRTP--TLRCLDMKALHKITVWFRVMMVYKSAIVSIRRHGVLVV 1119
E + SKSE M ++RTP L CL+ HKI V F+V K A V IR GV VV
Sbjct: 1182 PTMESWCGSKSEHMLLLRTPPSQLICLNENNRHKIEVSFQVRNYNKKAKVIIRSLGVYVV 1185
BLAST of Lcy02g011350 vs. NCBI nr
Match:
XP_008444002.1 (PREDICTED: TMV resistance protein N [Cucumis melo] >KAA0046813.1 TMV resistance protein N [Cucumis melo var. makuwa])
HSP 1 Score: 1057.4 bits (2733), Expect = 9.0e-305
Identity = 609/1039 (58.61%), Postives = 741/1039 (71.32%), Query Frame = 0
Query: 24 RKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSH-VVSNGVQMVGIYG 83
+ E+ LI D+V++V S + TQLL+VA HPVGIDSQL+A+ E SH V+ NGV MVGI+G
Sbjct: 170 KNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFASHGVLDNGVNMVGIHG 229
Query: 84 MGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILK-VNLEVN 143
MGG+GKTTLAKALYNKI EFE FLSNVREAS+QFNGLVQLQE LL I K NL+V+
Sbjct: 230 MGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQEKLLSEIFKDNNLKVD 289
Query: 144 NVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSH 203
NV +G+ I++DRLCS+KVLIVLDDVDK DQL+ LVGGRDWFGRGSKIIVTTR++ LL +
Sbjct: 290 NVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLERY 349
Query: 204 GFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLC 263
FDK+H IQ LD K+LELF WHAFK++HPS +Y+DLS+ YC GLPLALV+LGS LC
Sbjct: 350 SFDKIHPIQLLDYVKSLELFCWHAFKQNHPSRDYLDLSELVVRYCNGLPLALVILGSLLC 409
Query: 264 NRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDK--VKDIFLDISCFFVGEDVDRIKD 323
RDQ W+S L E +N I+ + QISF LE+ VK+IFLDI CFFVGEDV K+
Sbjct: 410 KRDQKIWKSKLDELKNFPEPGIEAVFQISFKRLEENPPVKEIFLDICCFFVGEDVSYSKN 469
Query: 324 MLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLWVAK 383
+L+ACD I ILMDLSL+T+ED +++MHDLIRQMGQTIV +SS+PEK+SRLWVAK
Sbjct: 470 VLKACDPYLESRIIILMDLSLVTVEDGKIQMHDLIRQMGQTIVRCKSSKPEKRSRLWVAK 529
Query: 384 DIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRN-IKFSKNIF 443
+ + ML E SGT VKAIKLDL + + ++ +AF+ M+NLRLLI++N KF NIF
Sbjct: 530 EAVKMLIEKSGTHKVKAIKLDLR---SNRPLIVEAEAFRNMENLRLLILQNAAKFPTNIF 589
Query: 444 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHVDFS 503
+ LPN +KWIE S FPIS VN LVGL + + G I E+ + LKHVD S
Sbjct: 590 KYLPN-IKWIEYSSSNVQWYFPISFVVNGGLVGLVINGVSNKHPGIIFEDCKMLKHVDLS 649
Query: 504 GSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPSYF- 563
+LEE DFS ALNL++LYLR C L+MIH S+ SLSKL+TLD +GC NL+KLPS F
Sbjct: 650 YWRLLEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFL 709
Query: 564 MLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNH 623
MLKSLEVLNL+ CI+L+EIPDLS +S+LKEL L EC NLRIIH S R L+KLV LD
Sbjct: 710 MLKSLEVLNLSGCIKLKEIPDLSASSSLKELHLRECYNLRIIHDSVGRFLDKLVILDFEG 769
Query: 624 CDNVKRLPCYV-MSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGCE 683
C N++RLP Y+ S S+E+ L + ++ D+YF KFPS+LK E LKVLNL C+
Sbjct: 770 CRNLERLPRYISKSGSIEVLNLDSCR-KIEQIFDNYFE--KFPSHLKYESLKVLNLSYCQ 829
Query: 684 NLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN 743
NL+ ITDFS +SNLEIL+L
Sbjct: 830 NLKGITDFSFASNLEILDLRG--------------------------------------- 889
Query: 744 LKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIKLE 803
C +LRT+HES+GSLDKL+ L+L C+ LE+LP+ L+LKSLDSL+L C KLE
Sbjct: 890 --------CFSLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSLTNCYKLE 949
Query: 804 QLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLY 863
QLPEFD+NMKSLR+M L T I++LP SI +LIGL+ L+L CTNLI+LPSEIHLLKSL
Sbjct: 950 QLPEFDENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEIHLLKSLK 1009
Query: 864 ELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSNLN 923
EL L ECS+LDMFP SSLN+ Q+SL S LTIL+L+NCNISN DFLE LSN ++L LN
Sbjct: 1010 ELDLHECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFCTTLKELN 1069
Query: 924 LSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYIAD 983
LSGNKF LPSLQNF SL+ LELR+CKFL+NI P HCL +VDA GCE VISPDYIAD
Sbjct: 1070 LSGNKFCSLPSLQNFSSLRHLELRNCKFLRNIVKIP-HCLTRVDASGCELFVISPDYIAD 1129
Query: 984 IIFRPQDNLERHRSRRLILMNSEIPNYFNNQTTESSVSVSFEHDNNPNKKQALAVCVVFK 1043
I+FR QD R+ R LI+ S+IP + NNQTTESS S SF+ N+ AL VCVVFK
Sbjct: 1130 IMFRNQDLELRNFKRELIVAYSKIPKFCNNQTTESSTSFSFQ-QNSDTIIPALVVCVVFK 1151
Query: 1044 VDGDSCEATAAIDCDVYVD 1055
VD DSC+A I V +D
Sbjct: 1190 VDEDSCDAEGFIRFQVLID 1151
BLAST of Lcy02g011350 vs. NCBI nr
Match:
KGN51651.2 (hypothetical protein Csa_008503 [Cucumis sativus])
HSP 1 Score: 1046.2 bits (2704), Expect = 2.1e-301
Identity = 587/968 (60.64%), Postives = 699/968 (72.21%), Query Frame = 0
Query: 18 YDVLNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAM-ELVSHVVSNGVQM 77
+D+ + E+DLI D+VKEVLS +N TQLLHVA HPVGIDSQL A+ EL SH V +GV M
Sbjct: 163 WDLATRKNEADLIHDLVKEVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNM 222
Query: 78 VGIYGMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN 137
VGI+GMGG+GKTTLAKALYNKIA +FE FLSNVRE +QF LVQLQE LL ILK N
Sbjct: 223 VGIHGMGGIGKTTLAKALYNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDN 282
Query: 138 L-EVNNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQ 197
+V NV +G IIRDRLCSKKVLI+LDDVDK +QL+ LVG RDWFGRGSKII TTR++
Sbjct: 283 AWKVGNVHKGKNIIRDRLCSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRH 342
Query: 198 LLSSHGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVL 257
LL +H FD ++ IQ LD K+LELFS HAFK++HPSSNYVDLSK A SYCKGLPLALV+L
Sbjct: 343 LLENHSFDIVYPIQLLDPKKSLELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVIL 402
Query: 258 GSSLCNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDR 317
GS L R++ W+S L+E ENSL ++ + QI F L ++VK+IFLDISCFFVGED++
Sbjct: 403 GSLLHKRERKIWKSKLHELENSLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINY 462
Query: 318 IKDMLEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPEKKSRLW 377
KD+L+ACDLNP+YGI ILMDLSL+T+ED +++MHDLI+QMGQTIV ES EP K+SRLW
Sbjct: 463 SKDVLKACDLNPDYGIIILMDLSLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLW 522
Query: 378 VAKDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIK-FSK 437
A+ I +LKE SGT AVKAIKLDL + K+V + +AF+ MKNLRLLI++ + F K
Sbjct: 523 EAEGAIKILKEKSGTKAVKAIKLDLHYKPWLKIV--EAEAFRNMKNLRLLILQRVAYFPK 582
Query: 438 NIFECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKILENSRRLKHV 497
NIFE LPN LKWIE S IS V +LVGL +K ++ EN + +KHV
Sbjct: 583 NIFEYLPNSLKWIEWSTFYVNQSSSISFSVKGRLVGLVMKGVVNKQPRIAFENCKTMKHV 642
Query: 498 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLP- 557
D S L+E +FSA LNL++LYLR C SLK+IHESV SLSKL+TLD +GC NL+K P
Sbjct: 643 DLSYCGTLKETPNFSATLNLEKLYLRGCTSLKVIHESVASLSKLVTLDLEGCDNLEKFPS 702
Query: 558 SYFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLD 617
SY MLKSLEVLNL++C ++EEIPDLS +SNLKEL L EC LRIIH S RSL+KL+ LD
Sbjct: 703 SYLMLKSLEVLNLSRCRKIEEIPDLSASSNLKELYLRECDRLRIIHDSIGRSLDKLIILD 762
Query: 618 LNHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCG 677
L C N++RLP Y + LE L N L LE S KFPS+LK + LKVLNL
Sbjct: 763 LEGCKNLERLPIY--TNKLESLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRD 822
Query: 678 CENLEEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAA 737
C NLEEITDFS++SNLEIL+L C +LR+I
Sbjct: 823 CLNLEEITDFSMASNLEILDLNTCFSLRII------------------------------ 882
Query: 738 LNLKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDSLNLDGCIK 797
HESIGSLDKL+TL+L C+NLEKLP+ LKLKSLDSL+ C K
Sbjct: 883 -----------------HESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYK 942
Query: 798 LEQLPEFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKS 857
LEQLPEFD+NMKSLR M L+ T I+ LP SIG+LIGL+ L+L C NL ALP+EIH LKS
Sbjct: 943 LEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKS 1002
Query: 858 LYELCLRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISNVDFLETLSNVSSSLSN 917
L EL LR CSKLDMFP RSSLN+SQES KLT+L+L+NCNISN DFLETLSNV +SL
Sbjct: 1003 LEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEK 1062
Query: 918 LNLSGNKFSCLPSLQNFKSLKSLELRHCKFLQNIANFPTHCLEKVDARGCESLVISPDYI 977
LNLSGN FSCLPSLQNFKSL+ LELR+CKFLQNI P H L +V+A G E L I PD I
Sbjct: 1063 LNLSGNTFSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHH-LARVNASGSELLAIRPDCI 1078
Query: 978 ADIIFRPQ 982
AD++F Q
Sbjct: 1123 ADMMFGKQ 1078
BLAST of Lcy02g011350 vs. TAIR 10
Match:
AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )
HSP 1 Score: 414.1 bits (1063), Expect = 3.7e-115
Identity = 330/1065 (30.99%), Postives = 538/1065 (50.52%), Query Frame = 0
Query: 23 NRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMVGIYGM 82
N +S LI+ IVK++ K+ T + +G+ S ++ ++ + +V V+M+GI+GM
Sbjct: 156 NWDDSKLIKKIVKDISDKLVSTS-WDDSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGM 215
Query: 83 GGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKV-NLEVNN 142
GG+GKTT+AK LYN+++ +F+ H F+ NV+E ++ G+ +LQ L + + + E +
Sbjct: 216 GGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVCNRY-GVRRLQVEFLCRMFQERDKEAWS 275
Query: 143 VDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSHG 202
II++R K V IVLDDVD+ +QL +LV WFG GS+IIVTTR++ LL SHG
Sbjct: 276 SVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHG 335
Query: 203 FDKMHDIQGLDQDKALELFSWHAFKES-HPSSNYVDLSKRATSYCKGLPLALVVLGSSLC 262
+ ++ ++ L + +AL+LF +AF+E + +LS +A +Y GLPLAL VLGS L
Sbjct: 336 INLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLY 395
Query: 263 NRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDRIKDML 322
R Q +W+S L + DI ++L++S++GL+++ K IFL ISCF+ + VD ++ +L
Sbjct: 396 RRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLL 455
Query: 323 EACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRES-SEPEKKSRLWVAKD 382
+ C GITIL + SLI + +K+HDL+ QMG+ +V +++ + P ++ LW +D
Sbjct: 456 DLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPED 515
Query: 383 IINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIKFSKNIFEC 442
I ++L ENSGT V+ I L+L + + V +AF+ + NL+LL ++ F
Sbjct: 516 ICHLLSENSGTQLVEGISLNLSEISE---VFASDRAFEGLSNLKLLNFYDLSFDGETRVH 575
Query: 443 LPNKLKWI-ECCRSIGFPSFPI----SSYVNDQLVGLDVKHSFVERLGKILENSRRLKHV 502
LPN L ++ R + + +P+ S + + LV L + +S +E+L ++ R LK +
Sbjct: 576 LPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKM 635
Query: 503 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKKLPS 562
D S L E+ D S A NL+EL L C SL + S+ +L L C+ LK +P
Sbjct: 636 DLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPI 695
Query: 563 YFMLKSLEVLNLTKCIQLEEIPDLSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDL 622
+LKSLE + ++ C L+ P++S N + L LS + + + S SR L+ LV LD+
Sbjct: 696 GIILKSLETVGMSGCSSLKHFPEIS--WNTRRLYLSS-TKIEELPSSISR-LSCLVKLDM 755
Query: 623 NHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNLCGC 682
+ C ++ LP Y+ L LK LNL GC
Sbjct: 756 SDCQRLRTLPSYLG---------------------------------HLVSLKSLNLDGC 815
Query: 683 ENLEEITD-FSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAA 742
LE + D ++LE L ++ C+N ++E R +
Sbjct: 816 RRLENLPDTLQNLTSLETLEVSGCLN---VNEFPR-----------------------VS 875
Query: 743 LNLKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYL-KLKSLDSLNLDGCI 802
++++L +S+ ++ + I +L +L +L++ L LP + +L+SL+ L L GC
Sbjct: 876 TSIEVLRISE-TSIEEIPARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCS 935
Query: 803 KLEQLP-EFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLL 862
LE P E + M LR LD+T IKELP +IG+L+ L+ L T + P I L
Sbjct: 936 VLESFPLEICQTMSCLRWFDLDRTSIKELPENIGNLVALEVLQASR-TVIRRAPWSIARL 995
Query: 863 KSLYELCLRECSKLDMFPQRSSLNYSQESLCSKLTIL-NLENCNISNVDFLETLSNVSS- 922
L L + F L+ SLC L+ +L ++SN++ E +++ +
Sbjct: 996 TRLQVLAIGN----SFFTPEGLLH----SLCPPLSRFDDLRALSLSNMNMTEIPNSIGNL 1055
Query: 923 -SLSNLNLSGNKFSCLP-SLQNFKSLKSLELRHCKFLQNIAN------------------ 982
+L L+LSGN F +P S++ L L L +C+ LQ + +
Sbjct: 1056 WNLLELDLSGNNFEFIPASIKRLTRLNRLNLNNCQRLQALPDELPRGLLYIYIHSCTSLV 1115
Query: 983 -----FPTHCLEKVDARGCESLVISPDYIADIIFRPQDNLERHRSRRLILMNSEIPNYFN 1042
F +CL K+ A C L D A I+ LE + S+IP FN
Sbjct: 1116 SISGCFNQYCLRKLVASNCYKL----DQAAQILIHRNLKLESAKPEHSYFPGSDIPTCFN 1138
Query: 1043 NQTTESSVSVSFEHDNNPNKKQALAVCVVFKVDGDSCEATAAIDC 1050
+Q S+++ + + + C++ VDG I C
Sbjct: 1176 HQVMGPSLNIQLPQSESSSDILGFSACIMIGVDGQYPMNNLKIHC 1138
BLAST of Lcy02g011350 vs. TAIR 10
Match:
AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 405.6 bits (1041), Expect = 1.3e-112
Identity = 285/786 (36.26%), Postives = 440/786 (55.98%), Query Frame = 0
Query: 21 LNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMVGIY 80
+ NR E++ I DI +E+L ++ Q LHV + VG+ S+L+ + + + S+GV+++ IY
Sbjct: 157 IKNRNEAECIADITREILKRL-PCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIY 216
Query: 81 GMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN-LEV 140
GMGG+GKTTLAK +N+ ++ FEG SFL N RE SK+ G LQ LL IL+ N +E
Sbjct: 217 GMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEF 276
Query: 141 NNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSS 200
+D +++R SK+VL+V+DDVD + QL RD FG GS+II+TTRN LL
Sbjct: 277 KGLDHA---VKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQ 336
Query: 201 HGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSL 260
+ + + LD D++LELFSWHAF+ S P ++ S+ +YC GLPLA+ VLG+ L
Sbjct: 337 LRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFL 396
Query: 261 CNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDRIKDM 320
R +W+S L + +I+ LQISFN L + KD+FLDI+CFF+G D + +
Sbjct: 397 IERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACI 456
Query: 321 LEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPE--KKSRLWVA 380
L+ C+L P+ +++LM+ LITI N + MHDL+R MG+ IV RE S + ++SRLW
Sbjct: 457 LDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIV-REISPKKCGERSRLWSH 516
Query: 381 KDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIKFSKNIF 440
D++ +LK+ SGT+A++ + L + + +AF KM+ LRLL +R + + + +
Sbjct: 517 NDVVGVLKKKSGTNAIEGLSLKADVM---DFQYFEVEAFAKMQELRLLELRYVDLNGS-Y 576
Query: 441 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKIL---ENSRRLKHV 500
E P L+W+ C FPI+ + + L LD+++S ++R K + + +K++
Sbjct: 577 EHFPKDLRWL-CWHGFSLECFPINLSL-ESLAALDLQYSNLKRFWKAQSPPQPANMVKYL 636
Query: 501 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSL-SKLITLDFQGCVNLKKLP 560
D S S L E DFS N+++L L +C SL ++H+S+G L KL+ L+ C+ L LP
Sbjct: 637 DLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLP 696
Query: 561 -SYFMLKSLEVLNLTKCIQLEEIPD-LSVASNLKELRLSECSNLRIIHGSTSRSLNKLVT 620
+ LKSLE L L+ C +LE + D L +L L L++ + LR I ST L KL
Sbjct: 697 EEIYKLKSLESLFLSNCSKLERLDDALGELESLTTL-LADFTALREI-PSTINQLKKLKR 756
Query: 621 LDLNHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNL 680
L LN C +L++ + L EK SL + S L Y+++L+L
Sbjct: 757 LSLNGCKG----------------LLSDDIDNLYSEKSHSVSLLRPVSLSGLTYMRILSL 816
Query: 681 CGCENL------EEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLE 740
C NL E+I S +L++ + C NL + +L +L+ L +C L+
Sbjct: 817 GYC-NLSDELIPEDIGSLSFLRDLDLRGNSFC-NLPTDFATLPNLGELL---LSDCSKLQ 876
Query: 741 MLPSFSAALNLKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDS 792
+ S +L L++ KCI L+ + I L L+L C +L ++P + L
Sbjct: 877 SI--LSLPRSLLFLDVGKCIMLKRTPD-ISKCSALFKLQLNDCISLFEIPGIHNHEYLSF 905
BLAST of Lcy02g011350 vs. TAIR 10
Match:
AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 405.6 bits (1041), Expect = 1.3e-112
Identity = 285/786 (36.26%), Postives = 440/786 (55.98%), Query Frame = 0
Query: 21 LNNRKESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAMELVSHVVSNGVQMVGIY 80
+ NR E++ I DI +E+L ++ Q LHV + VG+ S+L+ + + + S+GV+++ IY
Sbjct: 160 IKNRNEAECIADITREILKRL-PCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIY 219
Query: 81 GMGGLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVN-LEV 140
GMGG+GKTTLAK +N+ ++ FEG SFL N RE SK+ G LQ LL IL+ N +E
Sbjct: 220 GMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEF 279
Query: 141 NNVDRGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSS 200
+D +++R SK+VL+V+DDVD + QL RD FG GS+II+TTRN LL
Sbjct: 280 KGLDHA---VKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQ 339
Query: 201 HGFDKMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSL 260
+ + + LD D++LELFSWHAF+ S P ++ S+ +YC GLPLA+ VLG+ L
Sbjct: 340 LRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFL 399
Query: 261 CNRDQSKWQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDVDRIKDM 320
R +W+S L + +I+ LQISFN L + KD+FLDI+CFF+G D + +
Sbjct: 400 IERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACI 459
Query: 321 LEACDLNPNYGITILMDLSLITIEDNRLKMHDLIRQMGQTIVCRESSEPE--KKSRLWVA 380
L+ C+L P+ +++LM+ LITI N + MHDL+R MG+ IV RE S + ++SRLW
Sbjct: 460 LDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIV-REISPKKCGERSRLWSH 519
Query: 381 KDIINMLKENSGTDAVKAIKLDLPFLVQEKLVPIDPKAFKKMKNLRLLIIRNIKFSKNIF 440
D++ +LK+ SGT+A++ + L + + +AF KM+ LRLL +R + + + +
Sbjct: 520 NDVVGVLKKKSGTNAIEGLSLKADVM---DFQYFEVEAFAKMQELRLLELRYVDLNGS-Y 579
Query: 441 ECLPNKLKWIECCRSIGFPSFPISSYVNDQLVGLDVKHSFVERLGKIL---ENSRRLKHV 500
E P L+W+ C FPI+ + + L LD+++S ++R K + + +K++
Sbjct: 580 EHFPKDLRWL-CWHGFSLECFPINLSL-ESLAALDLQYSNLKRFWKAQSPPQPANMVKYL 639
Query: 501 DFSGSFVLEEILDFSAALNLKELYLRDCGSLKMIHESVGSL-SKLITLDFQGCVNLKKLP 560
D S S L E DFS N+++L L +C SL ++H+S+G L KL+ L+ C+ L LP
Sbjct: 640 DLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLP 699
Query: 561 -SYFMLKSLEVLNLTKCIQLEEIPD-LSVASNLKELRLSECSNLRIIHGSTSRSLNKLVT 620
+ LKSLE L L+ C +LE + D L +L L L++ + LR I ST L KL
Sbjct: 700 EEIYKLKSLESLFLSNCSKLERLDDALGELESLTTL-LADFTALREI-PSTINQLKKLKR 759
Query: 621 LDLNHCDNVKRLPCYVMSKSLELFILTNFMIRLNLEKDSYFSLAKFPSNLKLEYLKVLNL 680
L LN C +L++ + L EK SL + S L Y+++L+L
Sbjct: 760 LSLNGCKG----------------LLSDDIDNLYSEKSHSVSLLRPVSLSGLTYMRILSL 819
Query: 681 CGCENL------EEITDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLE 740
C NL E+I S +L++ + C NL + +L +L+ L +C L+
Sbjct: 820 GYC-NLSDELIPEDIGSLSFLRDLDLRGNSFC-NLPTDFATLPNLGELL---LSDCSKLQ 879
Query: 741 MLPSFSAALNLKLLNLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLPTYLKLKSLDS 792
+ S +L L++ KCI L+ + I L L+L C +L ++P + L
Sbjct: 880 SI--LSLPRSLLFLDVGKCIMLKRTPD-ISKCSALFKLQLNDCISLFEIPGIHNHEYLSF 908
BLAST of Lcy02g011350 vs. TAIR 10
Match:
AT1G27170.1 (transmembrane receptors;ATP binding )
HSP 1 Score: 390.2 bits (1001), Expect = 5.8e-108
Identity = 326/1011 (32.25%), Postives = 528/1011 (52.23%), Query Frame = 0
Query: 25 KESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAME-LVSHVVSNGVQMVGIYGMG 84
K+ D+I+ +VK VL+++++T V VG++S L+ + L+ S+GVQ++G+YGMG
Sbjct: 162 KDDDMIELVVKRVLAELSNTP-EKVGEFIVGLESPLKDLTGLIDTESSSGVQVLGLYGMG 221
Query: 85 GLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVNLEVNNVD 144
G+GKTTLAKA YNKI FE +F+S++RE S NGLV LQ+ L+ + ++ E+ +V
Sbjct: 222 GIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKELFRLVPEIEDVS 281
Query: 145 RGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSHGFD 204
G+ I+ + KK+++VLDDVD +DQ+ LVG W+G+G+ I++TTR+ ++LS +
Sbjct: 282 IGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITTRDSEILSKLSVN 341
Query: 205 KMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLCNRD 264
+ ++++ L + +AL+LFS+H+ ++ P+ N + LSK+ LPLA+ V GS L ++
Sbjct: 342 QQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLAVEVFGSLLYDKK 401
Query: 265 QSK-WQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDV--DRIKDML 324
+ K WQ+ L + + + +++D+L++SF L+D+ K +FLDI+C F+ ++ D + +L
Sbjct: 402 EEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDEEKKVFLDIACLFLKMEIKKDEVVIVL 461
Query: 325 EACDLNPNYGITILMDLSLITI-EDNRLKMHDLIRQMGQTIVCRESSE-PEKKSRLWVAK 384
+ C LN +++L SL+ I ++ L MHD IR MG+ +V +ES E P +SRLW
Sbjct: 462 KGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESREDPGLRSRLWDRG 521
Query: 385 DIINMLKENSGTDAVKAIKLD------------------------------------LPF 444
+I+ +L GT +++ I LD + F
Sbjct: 522 EIMTVLNNMKGTSSIRGIVLDFKKKFARDPTADEIVSRNLRNNPGIYSVFNYLKNKLVRF 581
Query: 445 LVQEK----LVPIDPKAFKKMKNLRLLIIRNIKFSKNIFECLPNKLKWIECCRSIGFPSF 504
+EK + I ++F M LRLL I N++ N+ + LP++LKWI+ + +
Sbjct: 582 PAEEKPKSSEITIPVESFAPMTKLRLLQINNVELEGNL-KLLPSELKWIQ-WKGCPLENL 641
Query: 505 PISSYVNDQLVGLDVKHSFVERLGKILEN--SRRLKHVDFSGSFVLEEILDFSAALNLKE 564
P ++ QL LD+ S + ++ + LK V G LE I D S L++
Sbjct: 642 P-PDFLARQLSVLDLSESGIRQVQTLRNKMVDENLKVVILRGCHSLEAIPDLSNHEALEK 701
Query: 565 LYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKK-LPSYFMLKSLEVLNLTKCIQLEEI 624
L C L + +SVG+L KLI LDF+ C L + L LK LE L L+ C L +
Sbjct: 702 LVFEQCTLLVKVPKSVGNLRKLIHLDFRRCSKLSEFLVDVSGLKLLEKLFLSGCSDLSVL 761
Query: 625 PD-LSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNHCDNVKRLP-CYVMSKSLE 684
P+ + ++LKEL L + + ++ + S +R L L L L C ++ LP C KSLE
Sbjct: 762 PENIGAMTSLKEL-LLDGTAIKNLPESINR-LQNLEILSLRGC-KIQELPLCIGTLKSLE 821
Query: 685 LFILTNFMIR--------LNLEKDSYF----SLAKFPSNL-KLEYLKVLNLCGCENLEEI 744
L + ++ L +D + SL+K P ++ +L+ LK L + G E
Sbjct: 822 KLYLDDTALKNLPSSIGDLKNLQDLHLVRCTSLSKIPDSINELKSLKKLFINGSAVEELP 881
Query: 745 TDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN-LKLL 804
S +L + DC L+ + S L+ L+ L + +E LP AL+ ++ L
Sbjct: 882 LKPSSLPSLYDFSAGDCKFLKQVPSSIGRLNSLLQLQL-SSTPIEALPEEIGALHFIREL 941
Query: 805 NLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLP-TYLKLKSLDSLNLDGCIKLEQLP 864
L C L+ + +SIG +D L +L L+ N+E+LP + KL+ L L + C L++LP
Sbjct: 942 ELRNCKFLKFLPKSIGDMDTLYSLNLEGS-NIEELPEEFGKLEKLVELRMSNCKMLKRLP 1001
Query: 865 EFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYELC 924
E ++KSL ++ + +T + ELP S G+L L L + L K L+ +
Sbjct: 1002 ESFGDLKSLHRLYMKETLVSELPESFGNLSNLMVLEM--------------LKKPLFRIS 1061
Query: 925 LRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISN--VDFLETLSNVSSSLSNLNL 967
P+ + S L KL L+ + IS D LE L S L LNL
Sbjct: 1062 ESNVPGTSEEPRFVEVPNSFSKLL-KLEELDACSWRISGKIPDDLEKL----SCLMKLNL 1121
BLAST of Lcy02g011350 vs. TAIR 10
Match:
AT1G27170.2 (transmembrane receptors;ATP binding )
HSP 1 Score: 390.2 bits (1001), Expect = 5.8e-108
Identity = 326/1011 (32.25%), Postives = 528/1011 (52.23%), Query Frame = 0
Query: 25 KESDLIQDIVKEVLSKVNHTQLLHVAMHPVGIDSQLEAME-LVSHVVSNGVQMVGIYGMG 84
K+ D+I+ +VK VL+++++T V VG++S L+ + L+ S+GVQ++G+YGMG
Sbjct: 162 KDDDMIELVVKRVLAELSNTP-EKVGEFIVGLESPLKDLTGLIDTESSSGVQVLGLYGMG 221
Query: 85 GLGKTTLAKALYNKIANEFEGHSFLSNVREASKQFNGLVQLQENLLYAILKVNLEVNNVD 144
G+GKTTLAKA YNKI FE +F+S++RE S NGLV LQ+ L+ + ++ E+ +V
Sbjct: 222 GIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKELFRLVPEIEDVS 281
Query: 145 RGITIIRDRLCSKKVLIVLDDVDKLDQLEKLVGGRDWFGRGSKIIVTTRNKQLLSSHGFD 204
G+ I+ + KK+++VLDDVD +DQ+ LVG W+G+G+ I++TTR+ ++LS +
Sbjct: 282 IGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITTRDSEILSKLSVN 341
Query: 205 KMHDIQGLDQDKALELFSWHAFKESHPSSNYVDLSKRATSYCKGLPLALVVLGSSLCNRD 264
+ ++++ L + +AL+LFS+H+ ++ P+ N + LSK+ LPLA+ V GS L ++
Sbjct: 342 QQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLAVEVFGSLLYDKK 401
Query: 265 QSK-WQSILYEFENSLTKDIKDILQISFNGLEDKVKDIFLDISCFFVGEDV--DRIKDML 324
+ K WQ+ L + + + +++D+L++SF L+D+ K +FLDI+C F+ ++ D + +L
Sbjct: 402 EEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDEEKKVFLDIACLFLKMEIKKDEVVIVL 461
Query: 325 EACDLNPNYGITILMDLSLITI-EDNRLKMHDLIRQMGQTIVCRESSE-PEKKSRLWVAK 384
+ C LN +++L SL+ I ++ L MHD IR MG+ +V +ES E P +SRLW
Sbjct: 462 KGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESREDPGLRSRLWDRG 521
Query: 385 DIINMLKENSGTDAVKAIKLD------------------------------------LPF 444
+I+ +L GT +++ I LD + F
Sbjct: 522 EIMTVLNNMKGTSSIRGIVLDFKKKFARDPTADEIVSRNLRNNPGIYSVFNYLKNKLVRF 581
Query: 445 LVQEK----LVPIDPKAFKKMKNLRLLIIRNIKFSKNIFECLPNKLKWIECCRSIGFPSF 504
+EK + I ++F M LRLL I N++ N+ + LP++LKWI+ + +
Sbjct: 582 PAEEKPKSSEITIPVESFAPMTKLRLLQINNVELEGNL-KLLPSELKWIQ-WKGCPLENL 641
Query: 505 PISSYVNDQLVGLDVKHSFVERLGKILEN--SRRLKHVDFSGSFVLEEILDFSAALNLKE 564
P ++ QL LD+ S + ++ + LK V G LE I D S L++
Sbjct: 642 P-PDFLARQLSVLDLSESGIRQVQTLRNKMVDENLKVVILRGCHSLEAIPDLSNHEALEK 701
Query: 565 LYLRDCGSLKMIHESVGSLSKLITLDFQGCVNLKK-LPSYFMLKSLEVLNLTKCIQLEEI 624
L C L + +SVG+L KLI LDF+ C L + L LK LE L L+ C L +
Sbjct: 702 LVFEQCTLLVKVPKSVGNLRKLIHLDFRRCSKLSEFLVDVSGLKLLEKLFLSGCSDLSVL 761
Query: 625 PD-LSVASNLKELRLSECSNLRIIHGSTSRSLNKLVTLDLNHCDNVKRLP-CYVMSKSLE 684
P+ + ++LKEL L + + ++ + S +R L L L L C ++ LP C KSLE
Sbjct: 762 PENIGAMTSLKEL-LLDGTAIKNLPESINR-LQNLEILSLRGC-KIQELPLCIGTLKSLE 821
Query: 685 LFILTNFMIR--------LNLEKDSYF----SLAKFPSNL-KLEYLKVLNLCGCENLEEI 744
L + ++ L +D + SL+K P ++ +L+ LK L + G E
Sbjct: 822 KLYLDDTALKNLPSSIGDLKNLQDLHLVRCTSLSKIPDSINELKSLKKLFINGSAVEELP 881
Query: 745 TDFSISSNLEILNLTDCINLRMIHESTRSLDKLVHCNLYNCKNLEMLPSFSAALN-LKLL 804
S +L + DC L+ + S L+ L+ L + +E LP AL+ ++ L
Sbjct: 882 LKPSSLPSLYDFSAGDCKFLKQVPSSIGRLNSLLQLQL-SSTPIEALPEEIGALHFIREL 941
Query: 805 NLSKCINLRTVHESIGSLDKLVTLELKFCYNLEKLP-TYLKLKSLDSLNLDGCIKLEQLP 864
L C L+ + +SIG +D L +L L+ N+E+LP + KL+ L L + C L++LP
Sbjct: 942 ELRNCKFLKFLPKSIGDMDTLYSLNLEGS-NIEELPEEFGKLEKLVELRMSNCKMLKRLP 1001
Query: 865 EFDKNMKSLRKMMLDQTGIKELPPSIGHLIGLQYLSLKGCTNLIALPSEIHLLKSLYELC 924
E ++KSL ++ + +T + ELP S G+L L L + L K L+ +
Sbjct: 1002 ESFGDLKSLHRLYMKETLVSELPESFGNLSNLMVLEM--------------LKKPLFRIS 1061
Query: 925 LRECSKLDMFPQRSSLNYSQESLCSKLTILNLENCNISN--VDFLETLSNVSSSLSNLNL 967
P+ + S L KL L+ + IS D LE L S L LNL
Sbjct: 1062 ESNVPGTSEEPRFVEVPNSFSKLL-KLEELDACSWRISGKIPDDLEKL----SCLMKLNL 1121
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
V9M2S5 | 3.3e-124 | 34.62 | Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... | [more] |
V9M398 | 5.1e-117 | 35.18 | Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... | [more] |
O23530 | 1.6e-102 | 30.49 | Protein SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=... | [more] |
A0A290U7C4 | 3.5e-102 | 32.12 | Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... | [more] |
Q40392 | 1.5e-97 | 33.58 | TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KNK0 | 0.0e+00 | 59.43 | TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G494390 PE=4 SV... | [more] |
A0A0A0LWV1 | 0.0e+00 | 58.19 | TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G334920 PE=4 SV... | [more] |
A0A5D3C521 | 0.0e+00 | 57.31 | TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... | [more] |
A0A5A7TTC7 | 4.4e-305 | 58.61 | TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... | [more] |
A0A1S3B9C2 | 4.4e-305 | 58.61 | TMV resistance protein N OS=Cucumis melo OX=3656 GN=LOC103487452 PE=4 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_031741747.1 | 0.0e+00 | 56.84 | TMV resistance protein N [Cucumis sativus] | [more] |
TYK06440.1 | 0.0e+00 | 57.31 | TMV resistance protein N [Cucumis melo var. makuwa] | [more] |
KGN65609.2 | 8.7e-308 | 58.19 | hypothetical protein Csa_019785 [Cucumis sativus] | [more] |
XP_008444002.1 | 9.0e-305 | 58.61 | PREDICTED: TMV resistance protein N [Cucumis melo] >KAA0046813.1 TMV resistance ... | [more] |
KGN51651.2 | 2.1e-301 | 60.64 | hypothetical protein Csa_008503 [Cucumis sativus] | [more] |
Match Name | E-value | Identity | Description | |
AT5G17680.1 | 3.7e-115 | 30.99 | disease resistance protein (TIR-NBS-LRR class), putative | [more] |
AT5G36930.1 | 1.3e-112 | 36.26 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT5G36930.2 | 1.3e-112 | 36.26 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT1G27170.1 | 5.8e-108 | 32.25 | transmembrane receptors;ATP binding | [more] |
AT1G27170.2 | 5.8e-108 | 32.25 | transmembrane receptors;ATP binding | [more] |