Lcy02g005460 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g005460
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
LocationChr02: 7152064 .. 7156580 (-)
RNA-Seq ExpressionLcy02g005460
SyntenyLcy02g005460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCTCCGGAAGTGGTGTAAGCTTAGTTACAGATTCTCAAATGGAGCTAGAAGTGGTCAATCATTTTTCTAATATTTATAAAGGCCCCACTTCTGAAGGATGGATGGTCTCAAATTTAGATTGGTGTCCTATCTCTAGTTCTGAGGCGACAGCTTTGATTCGCCCCTTTTCTGAGTTGGAGGTGTTTCAGAATCTAAAAGCGATGAGTCATAATAAGTCCCCTGGGCCGGATGGTTTCACTGTTGAATTCTTTAAAAATTCTTGGAACACTATCAAGCCCTTAGTTATGGAAGTGTTCCACGATTTTTATCAGAAAGGTATCATCAACTGTAATGTTAATGAAACCTACATTACTTTTGAAGGATTGGAATTCCCAATTCCGTTACAGCGGAAGCAATTGGACCGTTCGTAAATTAATTTTGTTCTACAAAATACCAATACATTGGCAAAAGGTTAAGCGGTGAAAAACATACTTCCGTTGACGACTCTGAATACTTCAACGGACACCACCATTTGATCTTCCCCGCTATAACTCTGAGATTAGGAACGTAGTTGTGGGACTACTTAATCTTGAATGGGAAAAAACTCTAGAGAGAAAAATTCTCCCTAAAGTTCTTGAGAAGTTCTTGAGTTGAGAGAATATGAGAGGACCTTATGGTGGCCATATGTTGTAGTACCTAAACCCTAGGGTTAGTGTTCTATTTATAGGAGAAAGGTCATGAATAAAATATTCATTTAAAATCAATCAATTCCATAACTGATTTAATATTTTCCTTAATTAATCAATTCCATAACCAATCAATTCCATAATTGATTAATCTTTTCCATAAATGATTGATAACTTCCATAAGTGATTAAATCATTTCCAAAAATGATTAATTACTTCCATAAGTGATTAATCATTTTCATAATTGATTAATTTCTAATTAATTAATTAATCACCAATTAAATATCATATATTTAATTTCAGACCTTCTATTAGTTAATTAATTAATTACCTAATTAAATATCATATATTTAATTTCAGGCCTTCAATTAATTAATTCTATAATATGAATCAAATTCATATTAAAATTGGAACTCCTTCCAATTCTCTTATATCCTATAAATTTAATATGAATCAAATTCATATTGATATTTGAACTTCTTCAAATATTTATTTCTCTCATAATAATAAAGTTTAATTTCAAATTAGACTTTATATTTTAATGTATCATTATACATTATATTAATTTGTATCTCATACAATTAACTTAATTCCCAATATGAATTTGAACTATTCAAATCCTCTCAAGATATAATTGTTCCTAATTACTCTTTACGAGCTAGCAGAGGGACCTAATGGACCTACAGATCAGAAGCTCCAATGATATGAGGTTAACCGATTAAACTCATTAATCAGGTTAACCAATATTCATTAACTGTGGGTCATTCCACTAAAGACCCACAGCTGAACTCTTCTCACTGTAGAACATTTCTGTGTCCACGGATATAGACCTATACCGGTAAGTCAATCCTTCACAAGTGTTCGTAACTACAGTTGGGTCAAATTACCGTTTTACCCCTGTGTTACATCTGTCTCCTTAAGTACCAGCGCTCCTCTAATGAACAACCTGTTTATGGTCCAACCATTAAACAGAAAGTTCCTCTCGGGTCAGTGAGAGGACGGGATCCCTTTGTTCAAGTCCCGGAGTCACCACTTAAGGGAACACTCATCTACTTCTCCTAGAAGTGAGAAGGAGTGAATTCCTTCTTGTGTTGTCATGTCCCCAGCCTCCCACTCGGTCTTGTCTCCAAAAAGGAAGGCATATTGAGTCGGCGTATTGGCCACTCTCACCCATGCTAATCAAAGGACAATCCCTCATGAGAAGGAGTTCATGACACACTCAGGATTACGACTGAGTTACCTAGGTCATCGTATTGAAACAGTAACCTAACTAGTTAACGGTGTTACATCTAGTGGTTTACTATTTCGTGGTCCGGTCTTATGCAAACTCATTGCATAGGATACCCCCGCTCGCATGTCTCCTACATGGACGCCTTGGATCAATACGTCTGTATCGAATACAGAGCGGGCCGTATCACATAGTGTTACCAAGATAAGGTACCCAGTCTTATCCCTGTACTATAGACCTTATAGGTTGTTACTTGAACACTGATCCCCGTATGTCCCCACATACCAGTTCAAGTCACATGTAAACAACCTTGGATCTTAGTTTATTGGATTTGGGTCAAATTGTGCAATATTTAACAATAACACTTTATTGTAGAAACTCAATAACACTTTATTGAAACAAGTATACAATTCGTTTACATTCACTATCTACGAGTTTTAGGGCATAAAACCCAACAACTTTGATACCTAAGAAGAATAAATCATTGCAAATTCAGGACTTTAGACCCATTAGTCTCACCACTGTTCTTTATCGCCTTATTGCTAAGACTCTTGCAGAAAGGCTTAAATGTACACTTCAAGGGACCATATCTGAGAATCAATTAGCCTTTGTAAAAGGTCGACAGATTACTGATGCTATTTTGGTGGCTAATGAAATCATTGATTTCTGGAAATGTTCTTGCACGAGAGGATTTGTTATAAAGCTTGATATTGAGAAAGCTTTTGACAAGATCTGCTGGAACTTTATTGATAAGACTCTCGCATTTAAAGGACACCCTATTACCTGGCGTAATTGGGTCAGGGCATGCATCACCTCAGTTTCATACTCCATATTGCTTAATGGGAAGCCAAGAGGCAAAATCAAGGCCGAAAGAGGCATTCGCCAAGGGGATCCGATCTCCCCTTTTATTTTTTTTCTTGCTATGAACTATCTTAGTCGGATTCTTCAGGCGGCCGAGCAAAAATGGCTTGTTAAGAGATGTTCTTTCAACTCTGTCTCCGTCTCTCATCTCCTTTTCGCAGATGACATACTCCTTTTTGTTCAAGATAATGATGCTATGATAGGCAACTTGTTCAACATCATCAAAGTCTTCGAGATATCTTCGGGTCTCAACATAAATTTTAATAAATCCTCTATTGCGGGTATCAATGTGGAGGATTCCAGAGTTGTTCAAATTGCCACTAATTGGGGATGCCCAATGGCCCATTTCCCCATTTCTTATTTGGGCTCTCCCTTGGGGGGTAATCCGTTATCGTCTCCTTCTTGGACGAATATGATTGAAAAGATTCATCGTAAGTTGGACAGTTGGCGCTTCTCCTATATTTCAAAGGGAGGAAGATTAACTTTGATTCAAGCGACCTTAAGAGGCATTCTCAACTATTTATTATCTATCTTTAAAGCTCCACAATCGGTATGCTCTAAAATAGATAAGATTATCAGATCTTTTCTTTGGCATGGGCAGGATAAAAGTAGTAGCATCCCTTTGGTTAATTGAAACTTGGTGGCTGCGCCTATTGAGGCTGGGGGTTTGGGCTTATTCAAGACTAGAATTACAAATAGCGCTTTTCAAGTTAAATGGCTTTGGAGATATTATCATGAGGAGATTTCTCTGTGGAAGCGGGTCATTTCAGCTAAATATACAGCCCATAGGCAGGGAGCTCTTCCAACTCAGACGCGATTCGCTTCCTCCCGAGCGCCATGGGCATCAATTCTCAAGCAAGCGTCTCTTTGTCTGGAGAATACAGCATGGAATCTAAAGGATGGCAGCAAAATCTCTTTTTGGCATGACTCATGGACCGATTTGGGGCCATTAAATCAAGCCCTCCCTCGTCTTTTCACGCTGTCCAATAGGAAAAATATGACAGTCTCGGATGCTTGGGACCACACCACTTCATCGTGGAAGCTTTATCCTAGGCGACCATTGTTTGACAGGGAGGTTTTTGCTCTAGCAGAACGCGCTTCCTGCTTTCCAACTCCTGCTTTATCCGGAGGGGTGGATTCCTTGCTATGGATCCCAGATTCTCAGGGTCGCTTCTCCGTTGCTTCTGCTAGGCGTTTCTTTTGGAGTTTGTCATCGACAGGTCGGCCCCAACCTCATTCGATTATTTTCTCAAATCTGTGGAAGGCTCTGATTCCAAAAAAGATCAAGTTCTTCATTTGGACTGTTATCTATAGAAGATTAAATACAACAGACAGGCTTCAACAAATTTTCAAATCCACTGCTTTGAACCCGAGCTGTTGCCCCCTTTGCCATGCTGGCTCGGACGCTCTGGATCACATATTTGTCCATTGTGGAGTTGCTTCTGATATTTGGCGCTCTTTTCATCTAGCTTTTGGTATTCATATGCCAATTCCTAGTCAAGTTAATTGGATATGTTTAGAGGCTTTCGCAGTCAAGGCTAATTCGCAGAGGGAGATTCTTATTCAGTCCATGTTTGCAGCGATATTATGGGTAATTTGGGGCGAGCGTAATAGGTGGATTTTTCAGAACACTTCTTGGACCAACATCAATATTTGGGAAGATATCATTACACTTGCGTCCTTTTGGGTATCCTCCACAAAAGCGTTCTCCAATTACCAGGCTTCATCAATTGCCTTGAATTGGAAAGCTTTTCTGTAG

mRNA sequence

ATGATCTCCGGAAGTGGTGTAAGCTTAGTTACAGATTCTCAAATGGAGCTAGAAGTGGTCAATCATTTTTCTAATATTTATAAAGGCCCCACTTCTGAAGGATGGATGGTCTCAAATTTAGATTGGTGTCCTATCTCTAGTTCTGAGGCGACAGCTTTGATTCGCCCCTTTTCTGAGTTGGAGCTTGATATTGAGAAAGCTTTTGACAAGATCTGCTGGAACTTTATTGATAAGACTCTCGCATTTAAAGGACACCCTATTACCTGGCGTAATTGGGTCAGGGCATGCATCACCTCAGTTTCATACTCCATATTGCTTAATGGGAAGCCAAGAGGCAAAATCAAGGCCGAAAGAGGCATTCGCCAAGGGGATCCGATCTCCCCTTTTATTTTTTTTCTTGCTATGAACTATCTTAGTCGGATTCTTCAGGCGGCCGAGCAAAAATGGCTTGTTAAGAGATGTTCTTTCAACTCTGTCTCCGTCTCTCATCTCCTTTTCGCAGATGACATACTCCTTTTTGTTCAAGATAATGATGCTATGATAGGCAACTTGTTCAACATCATCAAAGTCTTCGAGATATCTTCGGGTCTCAACATAAATTTTAATAAATCCTCTATTGCGGGTATCAATGTGGAGGATTCCAGAGTTGTTCAAATTGCCACTAATTGGGGATGCCCAATGGCCCATTTCCCCATTTCTTATTTGGGCTCTCCCTTGGGGGGTAATCCGTTATCGTCTCCTTCTTGGACGAATATGATTGAAAAGATTCATCCTAAATATACAGCCCATAGGCAGGGAGCTCTTCCAACTCAGACGCGATTCGCTTCCTCCCGAGCGCCATGGGCATCAATTCTCAAGCAAGCGTCTCTTTGTCTGGAGAATACAGCATGGAATCTAAAGGATGGCAGCAAAATCTCTTTTTGGCATGACTCATGGACCGATTTGGGGCCATTAAATCAAGCCCTCCCTCGTCTTTTCACGCTGTCCAATAGGAAAAATATGACAGTCTCGGATGCTTGGGACCACACCACTTCATCGTGGAAGCTTTATCCTAGGCGACCATTGTTTGACAGGGAGGTTTTTGCTCTAGCAGAACGCGCTTCCTGCTTTCCAACTCCTGCTTTATCCGGAGGGGTGGATTCCTTGCTATGGATCCCAGATTCTCAGGGTCGCTTCTCCGTTGCTTCTGCTAGGCGTTTCTTTTGGAGTTTGTCATCGACAGGTCGGCCCCAACCTCATTCGATTATTTTCTCAAATCTGTGGAAGGCTCTGATTCCAAAAAAGATCAAGTTCTTCATTTGGACTGTTATCTATAGAAGATTAAATACAACAGACAGGCTTCAACAAATTTTCAAATCCACTGCTTTGAACCCGAGCTGTTGCCCCCTTTGCCATGCTGGCTCGGACGCTCTGGATCACATATTTGTCCATTGTGGAGTTGCTTCTGATATTTGGCGCTCTTTTCATCTAGCTTTTGGTATTCATATGCCAATTCCTAGTCAAGTTAATTGGATATGTTTAGAGGCTTTCGCAGTCAAGGCTAATTCGCAGAGGGAGATTCTTATTCAGTCCATGTTTGCAGCGATATTATGGGTAATTTGGGGCGAGCGTAATAGGTGGATTTTTCAGAACACTTCTTGGACCAACATCAATATTTGGGAAGATATCATTACACTTGCGTCCTTTTGGGTATCCTCCACAAAAGCGTTCTCCAATTACCAGGCTTCATCAATTGCCTTGAATTGGAAAGCTTTTCTGTAG

Coding sequence (CDS)

ATGATCTCCGGAAGTGGTGTAAGCTTAGTTACAGATTCTCAAATGGAGCTAGAAGTGGTCAATCATTTTTCTAATATTTATAAAGGCCCCACTTCTGAAGGATGGATGGTCTCAAATTTAGATTGGTGTCCTATCTCTAGTTCTGAGGCGACAGCTTTGATTCGCCCCTTTTCTGAGTTGGAGCTTGATATTGAGAAAGCTTTTGACAAGATCTGCTGGAACTTTATTGATAAGACTCTCGCATTTAAAGGACACCCTATTACCTGGCGTAATTGGGTCAGGGCATGCATCACCTCAGTTTCATACTCCATATTGCTTAATGGGAAGCCAAGAGGCAAAATCAAGGCCGAAAGAGGCATTCGCCAAGGGGATCCGATCTCCCCTTTTATTTTTTTTCTTGCTATGAACTATCTTAGTCGGATTCTTCAGGCGGCCGAGCAAAAATGGCTTGTTAAGAGATGTTCTTTCAACTCTGTCTCCGTCTCTCATCTCCTTTTCGCAGATGACATACTCCTTTTTGTTCAAGATAATGATGCTATGATAGGCAACTTGTTCAACATCATCAAAGTCTTCGAGATATCTTCGGGTCTCAACATAAATTTTAATAAATCCTCTATTGCGGGTATCAATGTGGAGGATTCCAGAGTTGTTCAAATTGCCACTAATTGGGGATGCCCAATGGCCCATTTCCCCATTTCTTATTTGGGCTCTCCCTTGGGGGGTAATCCGTTATCGTCTCCTTCTTGGACGAATATGATTGAAAAGATTCATCCTAAATATACAGCCCATAGGCAGGGAGCTCTTCCAACTCAGACGCGATTCGCTTCCTCCCGAGCGCCATGGGCATCAATTCTCAAGCAAGCGTCTCTTTGTCTGGAGAATACAGCATGGAATCTAAAGGATGGCAGCAAAATCTCTTTTTGGCATGACTCATGGACCGATTTGGGGCCATTAAATCAAGCCCTCCCTCGTCTTTTCACGCTGTCCAATAGGAAAAATATGACAGTCTCGGATGCTTGGGACCACACCACTTCATCGTGGAAGCTTTATCCTAGGCGACCATTGTTTGACAGGGAGGTTTTTGCTCTAGCAGAACGCGCTTCCTGCTTTCCAACTCCTGCTTTATCCGGAGGGGTGGATTCCTTGCTATGGATCCCAGATTCTCAGGGTCGCTTCTCCGTTGCTTCTGCTAGGCGTTTCTTTTGGAGTTTGTCATCGACAGGTCGGCCCCAACCTCATTCGATTATTTTCTCAAATCTGTGGAAGGCTCTGATTCCAAAAAAGATCAAGTTCTTCATTTGGACTGTTATCTATAGAAGATTAAATACAACAGACAGGCTTCAACAAATTTTCAAATCCACTGCTTTGAACCCGAGCTGTTGCCCCCTTTGCCATGCTGGCTCGGACGCTCTGGATCACATATTTGTCCATTGTGGAGTTGCTTCTGATATTTGGCGCTCTTTTCATCTAGCTTTTGGTATTCATATGCCAATTCCTAGTCAAGTTAATTGGATATGTTTAGAGGCTTTCGCAGTCAAGGCTAATTCGCAGAGGGAGATTCTTATTCAGTCCATGTTTGCAGCGATATTATGGGTAATTTGGGGCGAGCGTAATAGGTGGATTTTTCAGAACACTTCTTGGACCAACATCAATATTTGGGAAGATATCATTACACTTGCGTCCTTTTGGGTATCCTCCACAAAAGCGTTCTCCAATTACCAGGCTTCATCAATTGCCTTGAATTGGAAAGCTTTTCTGTAG

Protein sequence

MISGSGVSLVTDSQMELEVVNHFSNIYKGPTSEGWMVSNLDWCPISSSEATALIRPFSELELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERGIRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDAMIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPLGGNPLSSPSWTNMIEKIHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKISFWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAERASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIPKKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWRSFHLAFGIHMPIPSQVNWICLEAFAVKANSQREILIQSMFAAILWVIWGERNRWIFQNTSWTNINIWEDIITLASFWVSSTKAFSNYQASSIALNWKAFL
Homology
BLAST of Lcy02g005460 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-13
Identity = 57/180 (31.67%), Postives = 83/180 (46.11%), Query Frame = 0

Query: 60  LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
           + +D EKAFDKI   F+ KTL   G    +   +RA     + +I+LNG+       + G
Sbjct: 597 ISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTG 656

Query: 120 IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            RQG P+SP +F + +  L+R   A  Q+  +K        V   LFADD+++++++   
Sbjct: 657 TRQGCPLSPLLFNIVLEVLAR---AIRQEKEIKGIQLGKEEVKLSLFADDMIVYLENPIV 716

Query: 180 MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
              NL  +I  F   SG  IN  KS     N       QI       +A   I YLG  L
Sbjct: 717 SAQNLLKLISNFSKVSGYKINVQKSQAFLYNNNRQTESQIMGELPFTIASKRIKYLGIQL 773

BLAST of Lcy02g005460 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 1.0e-12
Identity = 48/145 (33.10%), Postives = 72/145 (49.66%), Query Frame = 0

Query: 60  LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
           + LD EKAFDKI   F+ K L   G    + N ++A  +    +I +NG+    I  + G
Sbjct: 604 ISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSG 663

Query: 120 IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            RQG P+SP++F + +  L+R   A  Q+  +K        V   L ADD+++++ D   
Sbjct: 664 TRQGCPLSPYLFNIVLEVLAR---AIRQQKEIKGIQIGKEEVKISLLADDMIVYISDPKN 723

Query: 180 MIGNLFNIIKVFEISSGLNINFNKS 205
               L N+I  F    G  IN NKS
Sbjct: 724 STRELLNLINSFGEVVGYKINSNKS 745

BLAST of Lcy02g005460 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.5e-11
Identity = 45/145 (31.03%), Postives = 72/145 (49.66%), Query Frame = 0

Query: 60  LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
           L +D EKAFD I   F+ +TL   G   T+   + A  +  + +I+LNG          G
Sbjct: 596 LSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSG 655

Query: 120 IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            RQG P+SP +F + M  L+    A  ++  +K     S  +   LFADD+++++++   
Sbjct: 656 TRQGCPLSPLLFNIVMEVLA---IAIREEKAIKGIHIGSEEIKLSLFADDMIVYLENTRD 715

Query: 180 MIGNLFNIIKVFEISSGLNINFNKS 205
               L  +IK +   SG  IN +KS
Sbjct: 716 STTKLLEVIKEYSNVSGYKINTHKS 737

BLAST of Lcy02g005460 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.0e-09
Identity = 29/67 (43.28%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 105 LLNGKPRGKIKAERGIRQGDPISPFIFFLAMNYLSRILQAAEQKWLVK--RCSFNSVSVS 164
           ++NG P+G +   RG+RQGDP+SP++F L    LS + + A+++  +   R S NS  ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 165 HLLFADD 170
           HLLFADD
Sbjct: 73  HLLFADD 79

BLAST of Lcy02g005460 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-08
Identity = 42/151 (27.81%), Postives = 75/151 (49.67%), Query Frame = 0

Query: 55  RPFSELELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLN-GKPRGK 114
           + ++ + LD+ KAFD +  + I + L   G      N++   ++  + +I +  G    K
Sbjct: 135 KTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPGSQTRK 194

Query: 115 IKAERGIRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLF 174
           I   RG++QGDP+SPF+F   ++ L   LQ+          +     +  L FADD+LL 
Sbjct: 195 ICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTPG----IGGTIGEEKIPVLAFADDLLL- 254

Query: 175 VQDNDAMIGNLFNIIKVFEISSGLNINFNKS 205
           ++DND ++      +  F    G+++N  KS
Sbjct: 255 LEDNDVLLPTTLATVANFFRLRGMSLNAKKS 280

BLAST of Lcy02g005460 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 6.4e-111
Identity = 231/641 (36.04%), Postives = 320/641 (49.92%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            ++LDIEKAFDK+ W FID  L  KG+P  WRNW+RACI+SV YSI++NG+PRGKI+  RG
Sbjct: 420  IKLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRG 479

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            IRQGDPISPFIF LAM+Y+SR+L +  +K    +   N ++++HLLFADDILLFV+D++ 
Sbjct: 480  IRQGDPISPFIFVLAMDYMSRLLNSVGEKIKGVKLEGN-INLTHLLFADDILLFVEDDEH 539

Query: 180  MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
             I NL NII +F+++SGL+IN NKS+I+ INV+ SR  QIA+ WG      PI+YLG PL
Sbjct: 540  SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 599

Query: 240  GGNPLSSPSWTNMIEK-------------------------------------------- 299
            GG  ++   W N+ EK                                            
Sbjct: 600  GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTC 659

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 660  KNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIH 719

Query: 360  ---------IHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKIS 419
                     I+ KY +  +G +P     +SSR+PW SI K       + +W +K+G   S
Sbjct: 720  EDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFS 779

Query: 420  FWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAER 479
            FWH  W    PL+   PRL+ LS  K  ++ D W++T   W L PRR L + E    AE 
Sbjct: 780  FWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWEHPLWAEL 839

Query: 480  ASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIP 539
             +         G DS +W  +S G ++VAS ++                 F NLWK  IP
Sbjct: 840  KNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQPEQNLLDFQSQNTFKNLWKTSIP 899

Query: 540  KKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWR 586
            KK  FFIWT++Y  +NT ++L +   +    PS C +C    +   H+F+ C +A  IWR
Sbjct: 900  KKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWR 959

BLAST of Lcy02g005460 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 6.4e-111
Identity = 231/641 (36.04%), Postives = 320/641 (49.92%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            ++LDIEKAFDK+ W FID  L  KG+P  WRNW+RACI+SV YSI++NG+PRGKI+  RG
Sbjct: 420  IKLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRG 479

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            IRQGDPISPFIF LAM+Y+SR+L +  +K    +   N ++++HLLFADDILLFV+D++ 
Sbjct: 480  IRQGDPISPFIFVLAMDYMSRLLNSVGEKIKGVKLEGN-INLTHLLFADDILLFVEDDEH 539

Query: 180  MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
             I NL NII +F+++SGL+IN NKS+I+ INV+ SR  QIA+ WG      PI+YLG PL
Sbjct: 540  SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 599

Query: 240  GGNPLSSPSWTNMIEK-------------------------------------------- 299
            GG  ++   W N+ EK                                            
Sbjct: 600  GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTC 659

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 660  KNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIH 719

Query: 360  ---------IHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKIS 419
                     I+ KY +  +G +P     +SSR+PW SI K       + +W +K+G   S
Sbjct: 720  EDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFS 779

Query: 420  FWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAER 479
            FWH  W    PL+   PRL+ LS  K  ++ D W++T   W L PRR L + E    AE 
Sbjct: 780  FWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWEHPLWAEL 839

Query: 480  ASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIP 539
             +         G DS +W  +S G ++VAS ++                 F NLWK  IP
Sbjct: 840  KNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQPEQNLLDFQSQNTFKNLWKTSIP 899

Query: 540  KKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWR 586
            KK  FFIWT++Y  +NT ++L +   +    PS C +C    +   H+F+ C +A  IWR
Sbjct: 900  KKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWR 959

BLAST of Lcy02g005460 vs. ExPASy TrEMBL
Match: A0A5D3C4J1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00790 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 5.4e-110
Identity = 230/641 (35.88%), Postives = 319/641 (49.77%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            ++LDIEKAFDK+ W FID  L  KG+P  WR+W+RACI+SV YSI++NG+PRGKI+  RG
Sbjct: 483  IKLDIEKAFDKLNWRFIDFMLMKKGYPFKWRSWIRACISSVQYSIIINGRPRGKIQPSRG 542

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            IRQGDPISPFIF LAM+Y+SR+L +  +K    +   N ++++HLLFADDILLFV+D++ 
Sbjct: 543  IRQGDPISPFIFVLAMDYISRLLNSVGEKIKGVKLEGN-INLTHLLFADDILLFVEDDEH 602

Query: 180  MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
             I NL NII +F+++SGL+IN NKS+I+ INV+ SR  QIA+ WG      PI+YLG PL
Sbjct: 603  SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 662

Query: 240  GGNPLSSPSWTNMIEK-------------------------------------------- 299
            GG   +   W N+ EK                                            
Sbjct: 663  GGKQTTKAFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTC 722

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 723  KNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIH 782

Query: 360  ---------IHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKIS 419
                     I+ KY +  +G +P     +SSR+PW SI K       + +W +K+G   S
Sbjct: 783  EDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFS 842

Query: 420  FWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAER 479
            FWH  W    PL+   PRL+ LS  K  ++ D W++T   W L PRR L + E+   AE 
Sbjct: 843  FWHGHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWELPLWAEL 902

Query: 480  ASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIP 539
             +         G DS +W  +S G ++VAS ++       +         F NLWK  IP
Sbjct: 903  KNSINASFCENGKDSPMWALNSNGLYTVASVKKALQQPEQSLLDLQSQNTFKNLWKTSIP 962

Query: 540  KKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWR 586
            KK  FFIWT++Y  +NT ++L +        PS C +C    +   H+F+ C +A  IWR
Sbjct: 963  KKCIFFIWTLLYDSVNTAEQLMKRLPKLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWR 1022

BLAST of Lcy02g005460 vs. ExPASy TrEMBL
Match: A0A5A7US62 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003960 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 3.3e-107
Identity = 225/639 (35.21%), Postives = 317/639 (49.61%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            L+LDIEKAFDKI W+FID  LA K  P  WR W++ACI++V YSILLNG P+G+IKAERG
Sbjct: 1186 LKLDIEKAFDKISWSFIDYMLAKKHFPHKWRKWIKACISNVQYSILLNGAPKGRIKAERG 1245

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNS-VSVSHLLFADDILLFVQDND 179
            IRQGDP+SPFIF LAM+YLSR+L   E K  +K  SFN+  ++SHLLFADD+L+FV+DN+
Sbjct: 1246 IRQGDPLSPFIFVLAMDYLSRLLSHLESKGAIKGVSFNNCCNISHLLFADDVLIFVEDNE 1305

Query: 180  AMIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSP 239
              + NL   + +FE +SGL  N +KS+I+ IN+   R  QIA+ +G      P++YLG P
Sbjct: 1306 RYLNNLQMALTLFEKASGLTFNNSKSTISPINISAGRTDQIASFFGFQTKFLPVNYLGVP 1365

Query: 240  LGGNPLSSPSWTNMIEKIH----------------------------------------- 299
            LGGNP S   W   IE IH                                         
Sbjct: 1366 LGGNPRSRSFWDQTIECIHKKLNGWKYSQISKGGRLTLLKASLSSLPTYQLSTFKAPVSV 1425

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 1426 YKEIEKHWRDFLWGGSEDKQNAHLINWNICTSPKELGGLGISKLKDTNQALLCKWLWRYH 1485

Query: 360  ------------PKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKI 419
                         KYT + QG +P   R +S+ +PW +I K         +W   DGS +
Sbjct: 1486 NESNSLWKKCIDAKYTKNHQGDIPVVGRNSSANSPWNAIKKWKDWYESKISWLPNDGSSL 1545

Query: 420  SFWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAE 479
            SFWH  W +  PL+  +PRL+ LSN ++ TV + WD  +  W + PRRPL +RE      
Sbjct: 1546 SFWHSKWHNNIPLSLQIPRLYALSNMQSATVKEIWDQGSDDWNMKPRRPLNEREQQTWDS 1605

Query: 480  RASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALI 539
                 P    + G+    W P    +++VASA+   +  SS  +         +LW++ I
Sbjct: 1606 IKMSLPRIHNNRGMCKPTWNPSDSKKYTVASAKDIAFKESSIPKETNWEKELKHLWRSHI 1665

Query: 540  PKKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIW 585
            P+K KFFIWT+++++LNT D +Q+   S +LNPS C  C + ++ ++H+F+ C  A  +W
Sbjct: 1666 PQKCKFFIWTMVHQKLNTMDNIQKRNPSMSLNPSWCISCRSSNEDMNHLFIFCPFARSLW 1725

BLAST of Lcy02g005460 vs. ExPASy TrEMBL
Match: A0A5D3CA17 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00050 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 3.3e-107
Identity = 225/639 (35.21%), Postives = 317/639 (49.61%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            L+LDIEKAFDKI W+FID  LA K  P  WR W++ACI++V YSILLNG P+G+IKAERG
Sbjct: 1186 LKLDIEKAFDKISWSFIDYMLAKKHFPHKWRKWIKACISNVQYSILLNGAPKGRIKAERG 1245

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNS-VSVSHLLFADDILLFVQDND 179
            IRQGDP+SPFIF LAM+YLSR+L   E K  +K  SFN+  ++SHLLFADD+L+FV+DN+
Sbjct: 1246 IRQGDPLSPFIFVLAMDYLSRLLSHLESKGAIKGVSFNNYCNISHLLFADDVLIFVEDNE 1305

Query: 180  AMIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSP 239
              + NL   + +FE +SGL  N +KS+I+ IN+   R  QIA+ +G      P++YLG P
Sbjct: 1306 RYLNNLQMALTLFEKASGLTFNNSKSTISPINISAGRTDQIASFFGFQTKFLPVNYLGVP 1365

Query: 240  LGGNPLSSPSWTNMIEKIH----------------------------------------- 299
            LGGNP S   W   IE IH                                         
Sbjct: 1366 LGGNPRSRSFWDQTIECIHKKLNGWKYSQISKGGRLTLLKASLSSLPTYQLSTFKAPVSV 1425

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 1426 YKEIEKHWRDFLWGGSEDKQNAHLINWNICTSPKELGGLGISKLKDTNQALLCKWLWRYH 1485

Query: 360  ------------PKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKI 419
                         KYT + QG +P   R +S+ +PW +I K         +W   DGS +
Sbjct: 1486 NESNSLWKKCIDAKYTKNHQGDIPVVGRNSSANSPWNAIKKWKDWYESKISWLPNDGSSL 1545

Query: 420  SFWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAE 479
            SFWH  W +  PL+  +PRL+ LSN ++ TV + WD  +  W + PRRPL +RE      
Sbjct: 1546 SFWHSKWHNNIPLSLQIPRLYALSNMQSATVKEIWDQGSDDWNMKPRRPLNEREQQTWDS 1605

Query: 480  RASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALI 539
                 P    + G+    W P    +++VASA+   +  SS  +         +LW++ I
Sbjct: 1606 IKMSLPRIHNNRGMCKPTWNPSDSKKYTVASAKDIAFKESSIPKETNWEKELKHLWRSHI 1665

Query: 540  PKKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIW 585
            P+K KFFIWT+++++LNT D +Q+   S +LNPS C  C + ++ ++H+F+ C  A  +W
Sbjct: 1666 PQKCKFFIWTMVHQKLNTMDNIQKRNPSMSLNPSWCISCRSSNEDMNHLFIFCPFARSLW 1725

BLAST of Lcy02g005460 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-110
Identity = 231/641 (36.04%), Postives = 320/641 (49.92%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            ++LDIEKAFDK+ W FID  L  KG+P  WRNW+RACI+SV YSI++NG+PRGKI+  RG
Sbjct: 420  IKLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRG 479

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            IRQGDPISPFIF LAM+Y+SR+L +  +K    +   N ++++HLLFADDILLFV+D++ 
Sbjct: 480  IRQGDPISPFIFVLAMDYMSRLLNSVGEKIKGVKLEGN-INLTHLLFADDILLFVEDDEH 539

Query: 180  MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
             I NL NII +F+++SGL+IN NKS+I+ INV+ SR  QIA+ WG      PI+YLG PL
Sbjct: 540  SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 599

Query: 240  GGNPLSSPSWTNMIEK-------------------------------------------- 299
            GG  ++   W N+ EK                                            
Sbjct: 600  GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTC 659

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 660  KNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIH 719

Query: 360  ---------IHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKIS 419
                     I+ KY +  +G +P     +SSR+PW SI K       + +W +K+G   S
Sbjct: 720  EDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFS 779

Query: 420  FWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAER 479
            FWH  W    PL+   PRL+ LS  K  ++ D W++T   W L PRR L + E    AE 
Sbjct: 780  FWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWEHPLWAEL 839

Query: 480  ASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIP 539
             +         G DS +W  +S G ++VAS ++                 F NLWK  IP
Sbjct: 840  KNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQPEQNLLDFQSQNTFKNLWKTSIP 899

Query: 540  KKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWR 586
            KK  FFIWT++Y  +NT ++L +   +    PS C +C    +   H+F+ C +A  IWR
Sbjct: 900  KKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWR 959

BLAST of Lcy02g005460 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-110
Identity = 231/641 (36.04%), Postives = 320/641 (49.92%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            ++LDIEKAFDK+ W FID  L  KG+P  WRNW+RACI+SV YSI++NG+PRGKI+  RG
Sbjct: 420  IKLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRG 479

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            IRQGDPISPFIF LAM+Y+SR+L +  +K    +   N ++++HLLFADDILLFV+D++ 
Sbjct: 480  IRQGDPISPFIFVLAMDYMSRLLNSVGEKIKGVKLEGN-INLTHLLFADDILLFVEDDEH 539

Query: 180  MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
             I NL NII +F+++SGL+IN NKS+I+ INV+ SR  QIA+ WG      PI+YLG PL
Sbjct: 540  SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 599

Query: 240  GGNPLSSPSWTNMIEK-------------------------------------------- 299
            GG  ++   W N+ EK                                            
Sbjct: 600  GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTC 659

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 660  KNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIH 719

Query: 360  ---------IHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKIS 419
                     I+ KY +  +G +P     +SSR+PW SI K       + +W +K+G   S
Sbjct: 720  EDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFS 779

Query: 420  FWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAER 479
            FWH  W    PL+   PRL+ LS  K  ++ D W++T   W L PRR L + E    AE 
Sbjct: 780  FWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWEHPLWAEL 839

Query: 480  ASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIP 539
             +         G DS +W  +S G ++VAS ++                 F NLWK  IP
Sbjct: 840  KNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQPEQNLLDFQSQNTFKNLWKTSIP 899

Query: 540  KKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWR 586
            KK  FFIWT++Y  +NT ++L +   +    PS C +C    +   H+F+ C +A  IWR
Sbjct: 900  KKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWR 959

BLAST of Lcy02g005460 vs. NCBI nr
Match: TYK06777.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-109
Identity = 230/641 (35.88%), Postives = 319/641 (49.77%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            ++LDIEKAFDK+ W FID  L  KG+P  WR+W+RACI+SV YSI++NG+PRGKI+  RG
Sbjct: 483  IKLDIEKAFDKLNWRFIDFMLMKKGYPFKWRSWIRACISSVQYSIIINGRPRGKIQPSRG 542

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNSVSVSHLLFADDILLFVQDNDA 179
            IRQGDPISPFIF LAM+Y+SR+L +  +K    +   N ++++HLLFADDILLFV+D++ 
Sbjct: 543  IRQGDPISPFIFVLAMDYISRLLNSVGEKIKGVKLEGN-INLTHLLFADDILLFVEDDEH 602

Query: 180  MIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSPL 239
             I NL NII +F+++SGL+IN NKS+I+ INV+ SR  QIA+ WG      PI+YLG PL
Sbjct: 603  SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 662

Query: 240  GGNPLSSPSWTNMIEK-------------------------------------------- 299
            GG   +   W N+ EK                                            
Sbjct: 663  GGKQTTKAFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTC 722

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 723  KNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIH 782

Query: 360  ---------IHPKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKIS 419
                     I+ KY +  +G +P     +SSR+PW SI K       + +W +K+G   S
Sbjct: 783  EDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFS 842

Query: 420  FWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAER 479
            FWH  W    PL+   PRL+ LS  K  ++ D W++T   W L PRR L + E+   AE 
Sbjct: 843  FWHGHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWELPLWAEL 902

Query: 480  ASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALIP 539
             +         G DS +W  +S G ++VAS ++       +         F NLWK  IP
Sbjct: 903  KNSINASFCENGKDSPMWALNSNGLYTVASVKKALQQPEQSLLDLQSQNTFKNLWKTSIP 962

Query: 540  KKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIWR 586
            KK  FFIWT++Y  +NT ++L +        PS C +C    +   H+F+ C +A  IWR
Sbjct: 963  KKCIFFIWTLLYDSVNTAEQLMKRLPKLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWR 1022

BLAST of Lcy02g005460 vs. NCBI nr
Match: TYK08190.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 399.1 bits (1024), Expect = 6.8e-107
Identity = 225/639 (35.21%), Postives = 317/639 (49.61%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            L+LDIEKAFDKI W+FID  LA K  P  WR W++ACI++V YSILLNG P+G+IKAERG
Sbjct: 1186 LKLDIEKAFDKISWSFIDYMLAKKHFPHKWRKWIKACISNVQYSILLNGAPKGRIKAERG 1245

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNS-VSVSHLLFADDILLFVQDND 179
            IRQGDP+SPFIF LAM+YLSR+L   E K  +K  SFN+  ++SHLLFADD+L+FV+DN+
Sbjct: 1246 IRQGDPLSPFIFVLAMDYLSRLLSHLESKGAIKGVSFNNYCNISHLLFADDVLIFVEDNE 1305

Query: 180  AMIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSP 239
              + NL   + +FE +SGL  N +KS+I+ IN+   R  QIA+ +G      P++YLG P
Sbjct: 1306 RYLNNLQMALTLFEKASGLTFNNSKSTISPINISAGRTDQIASFFGFQTKFLPVNYLGVP 1365

Query: 240  LGGNPLSSPSWTNMIEKIH----------------------------------------- 299
            LGGNP S   W   IE IH                                         
Sbjct: 1366 LGGNPRSRSFWDQTIECIHKKLNGWKYSQISKGGRLTLLKASLSSLPTYQLSTFKAPVSV 1425

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 1426 YKEIEKHWRDFLWGGSEDKQNAHLINWNICTSPKELGGLGISKLKDTNQALLCKWLWRYH 1485

Query: 360  ------------PKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKI 419
                         KYT + QG +P   R +S+ +PW +I K         +W   DGS +
Sbjct: 1486 NESNSLWKKCIDAKYTKNHQGDIPVVGRNSSANSPWNAIKKWKDWYESKISWLPNDGSSL 1545

Query: 420  SFWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAE 479
            SFWH  W +  PL+  +PRL+ LSN ++ TV + WD  +  W + PRRPL +RE      
Sbjct: 1546 SFWHSKWHNNIPLSLQIPRLYALSNMQSATVKEIWDQGSDDWNMKPRRPLNEREQQTWDS 1605

Query: 480  RASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALI 539
                 P    + G+    W P    +++VASA+   +  SS  +         +LW++ I
Sbjct: 1606 IKMSLPRIHNNRGMCKPTWNPSDSKKYTVASAKDIAFKESSIPKETNWEKELKHLWRSHI 1665

Query: 540  PKKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIW 585
            P+K KFFIWT+++++LNT D +Q+   S +LNPS C  C + ++ ++H+F+ C  A  +W
Sbjct: 1666 PQKCKFFIWTMVHQKLNTMDNIQKRNPSMSLNPSWCISCRSSNEDMNHLFIFCPFARSLW 1725

BLAST of Lcy02g005460 vs. NCBI nr
Match: KAA0057507.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 399.1 bits (1024), Expect = 6.8e-107
Identity = 225/639 (35.21%), Postives = 317/639 (49.61%), Query Frame = 0

Query: 60   LELDIEKAFDKICWNFIDKTLAFKGHPITWRNWVRACITSVSYSILLNGKPRGKIKAERG 119
            L+LDIEKAFDKI W+FID  LA K  P  WR W++ACI++V YSILLNG P+G+IKAERG
Sbjct: 1186 LKLDIEKAFDKISWSFIDYMLAKKHFPHKWRKWIKACISNVQYSILLNGAPKGRIKAERG 1245

Query: 120  IRQGDPISPFIFFLAMNYLSRILQAAEQKWLVKRCSFNS-VSVSHLLFADDILLFVQDND 179
            IRQGDP+SPFIF LAM+YLSR+L   E K  +K  SFN+  ++SHLLFADD+L+FV+DN+
Sbjct: 1246 IRQGDPLSPFIFVLAMDYLSRLLSHLESKGAIKGVSFNNCCNISHLLFADDVLIFVEDNE 1305

Query: 180  AMIGNLFNIIKVFEISSGLNINFNKSSIAGINVEDSRVVQIATNWGCPMAHFPISYLGSP 239
              + NL   + +FE +SGL  N +KS+I+ IN+   R  QIA+ +G      P++YLG P
Sbjct: 1306 RYLNNLQMALTLFEKASGLTFNNSKSTISPINISAGRTDQIASFFGFQTKFLPVNYLGVP 1365

Query: 240  LGGNPLSSPSWTNMIEKIH----------------------------------------- 299
            LGGNP S   W   IE IH                                         
Sbjct: 1366 LGGNPRSRSFWDQTIECIHKKLNGWKYSQISKGGRLTLLKASLSSLPTYQLSTFKAPVSV 1425

Query: 300  ------------------------------------------------------------ 359
                                                                        
Sbjct: 1426 YKEIEKHWRDFLWGGSEDKQNAHLINWNICTSPKELGGLGISKLKDTNQALLCKWLWRYH 1485

Query: 360  ------------PKYTAHRQGALPTQTRFASSRAPWASILKQASLCLENTAWNLKDGSKI 419
                         KYT + QG +P   R +S+ +PW +I K         +W   DGS +
Sbjct: 1486 NESNSLWKKCIDAKYTKNHQGDIPVVGRNSSANSPWNAIKKWKDWYESKISWLPNDGSSL 1545

Query: 420  SFWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFDREVFALAE 479
            SFWH  W +  PL+  +PRL+ LSN ++ TV + WD  +  W + PRRPL +RE      
Sbjct: 1546 SFWHSKWHNNIPLSLQIPRLYALSNMQSATVKEIWDQGSDDWNMKPRRPLNEREQQTWDS 1605

Query: 480  RASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIFSNLWKALI 539
                 P    + G+    W P    +++VASA+   +  SS  +         +LW++ I
Sbjct: 1606 IKMSLPRIHNNRGMCKPTWNPSDSKKYTVASAKDIAFKESSIPKETNWEKELKHLWRSHI 1665

Query: 540  PKKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIFVHCGVASDIW 585
            P+K KFFIWT+++++LNT D +Q+   S +LNPS C  C + ++ ++H+F+ C  A  +W
Sbjct: 1666 PQKCKFFIWTMVHQKLNTMDNIQKRNPSMSLNPSWCISCRSSNEDMNHLFIFCPFARSLW 1725

BLAST of Lcy02g005460 vs. TAIR 10
Match: AT1G60720.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 72.4 bits (176), Expect = 1.4e-12
Identity = 69/251 (27.49%), Postives = 109/251 (43.43%), Query Frame = 0

Query: 298 NLKDGSKISFWHDSWTDLGPLNQALPRLFTLSNRKNMTVSDAWDHTTSSWKLYPRRPLFD 357
           NL +G    FWHDSWT LGPL + +    + S R  +          + WKL   R    
Sbjct: 16  NLGNGRIAHFWHDSWTSLGPLIKVMGDYGSRSLRIPLNARVVEALGVNGWKLPLSR---S 75

Query: 358 REVFALAERASCFPTPALSGGVDSLLWIPDSQGRFSVASARRFFWSLSSTGRPQPHSIIF 417
               A+ +  S   TP+ +   DS  W+         +SAR +        RP+   + +
Sbjct: 76  APAQAIHDHISTITTPSPATIEDSFDWVVGGVVCQGFSSARTW-----DAIRPRAPELDW 135

Query: 418 SN-LW-KALIPKKIKFFIWTVIYRRLNTTDRLQQIFKSTALNPSCCPLCHAGSDALDHIF 477
           +  +W K  +PK   F +W     RL T  RL       + +  CC LC   +++ DH+ 
Sbjct: 136 AKAVWFKGAVPKH-AFNMWISQLDRLPTRQRLASWGHIQSFD--CC-LCTIETESRDHLL 195

Query: 478 VHCGVASDIWRSFHLAFGIHMPIPSQ-VNWICLEAFAVKANSQREILIQSMFA-AILWVI 537
             C  A+ +WR   LAF    P      +W  L ++   ++S    L++ + A AI++ I
Sbjct: 196 FSCEFAAQVWR---LAFSRLCPRQRLFCSWAELLSWMRSSSSSAPSLLRKVSAHAIIYNI 251

Query: 538 WGERNRWIFQN 545
           W +RN  +  N
Sbjct: 256 WRQRNNVLHNN 251

BLAST of Lcy02g005460 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-10
Identity = 29/67 (43.28%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 105 LLNGKPRGKIKAERGIRQGDPISPFIFFLAMNYLSRILQAAEQKWLVK--RCSFNSVSVS 164
           ++NG P+G +   RG+RQGDP+SP++F L    LS + + A+++  +   R S NS  ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 165 HLLFADD 170
           HLLFADD
Sbjct: 73  HLLFADD 79

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O003701.2e-1331.67LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P113691.0e-1233.10LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085482.5e-1131.03LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
P925554.0e-0943.28Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
Q032741.2e-0827.81Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra... [more]
Match NameE-valueIdentityDescription
A0A5D3DM726.4e-11136.04LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S4E2K56.4e-11136.04LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC1079... [more]
A0A5D3C4J15.4e-11035.88LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7US623.3e-10735.21LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3CA173.3e-10735.21LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
XP_016902461.11.3e-11036.04PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo][more]
KAA0039770.11.3e-11036.04LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK247... [more]
TYK06777.11.1e-10935.88LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
TYK08190.16.8e-10735.21LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0057507.16.8e-10735.21LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G60720.11.4e-1227.49RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
ATMG01250.12.8e-1043.28RNA-directed DNA polymerase (reverse transcriptase) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 60..236
e-value: 1.8E-25
score: 89.7
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 1..239
score: 12.91392
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 405..485
e-value: 2.0E-14
score: 54.1
NoneNo IPR availablePANTHERPTHR33116:SF38OS01G0158850 PROTEINcoord: 292..518
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 292..518
NoneNo IPR availablePANTHERPTHR33116:SF38OS01G0158850 PROTEINcoord: 60..276
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 60..276
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 60..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g005460.1Lcy02g005460.1mRNA