Lcy01g015870 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g015870
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionL-ascorbate oxidase homolog
LocationChr01: 37411469 .. 37416193 (+)
RNA-Seq ExpressionLcy01g015870
SyntenyLcy01g015870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTACTCTCTCTGCACACTCGCTTAAATATAAAACCCCCCCTTTCTTCCTCTCTCACTCCTCCATAGCCATTTCTCTCTCTTCACCTCCATCTCCATTGCTCTCTTTCAGCAATGGCGTCTCCTTCACGCCGATCCTCTCTTCCACCTCTCCTCTGCCTCTGCATTTCCTTCTTCGCCATTGTTCGAGCCGACGATCCTTACAGATTCTTCGACTGGAACGTCACCTATGGCACCATTTACCCCCTCGGCGTCCCCCAACAGGTTAGGTTTTCGGTTGAATCATGTTGATTGTTTCTGTTCTTCCTTTTGGTTCCGTCCGACTCCGATTGAGAATGGATCTGAATCTGCTCTGTTTTTTTTAGGGTATTCTAATCAATGGCCAATTTCCTGGCCCCGATATTCACTCTGTTACCAATGATAACATCATTATCAATGTCTACAATAGCTTGGATGAGCCCTTTCTCCTCTCCTGGTAATTTATCTATCCCTCTCTCTCTCTCTCTCTCTGTGTAAACTTGACTCGAAATCTCCATTTTCGTTACTGAGTGTGAAAATGTGCTTTCTAGAACGCGCTATTGAGTGTTTGGTAGCTTTTGTAGACTTGGAGTTGAAATATTAAGAAGAAACAGAAAATTGATGGTGGTGTTAATTTGGTGAATTTGTCGGCATTGATTGCATCTCTCTCTCTGTCTGTCTTCAATAGCTAAATTTAGGGGTTGGATCTGGTTTAATTGTTAACTAGTTGAAGAATGTTTTGAGACAATGTCAGCAAATTATCACTGAATCTGGTTTGAATTGGAAGTTTTTAACTGAAAATTGGTGTTGTGAACTGTGAGATTAGGAATGGGATTCAACAGAGAAGGAACTCGTACGAAGACGGTGTGTACGGAACAACATGCCCAATTCCTCCAGGGAAGAACTTCACTTACATTCTCCAAATGAAAGATCAGATCGGAAGCTTCTATTACTTCCCTTCTCTCGGATTCCACAAGGCCGCCGGTGGTTTTGGTGGAATCAGAATTCTCAGCCGCCCCCGGATTCCCGTTCCCTTCCCGGATCCCGCCGGCGATTACACCGTCCTCATCGGCGACTGGTACCAAGCTAATCATACGGTAACTAATCACTAATCATCCCTTTTCCTTTATCTTTTCAAAATAAAGTGATTCTCTTTCTTCTTTAAGCTATATTCAGATTAAATCATTCATTAGTGTGAAAAGGCGAAGAAAATGAATATTAGTCACGGGATGAGCCAGATTGGCGGGGAATAGGAATAGGGAATCCTTCAATTTGACTTTTCTTTTTACCTCTGTCAGTGTCGGTAGACCATTGCTTCACTATACTGCCTGACCTGTAATCTTTGACTCTTCAACTGCTTTTTGCTTCTCTCTCTAGTCTCTCTCTACTGAGATTAACAGCTGGATTACTTAAAGGGACTTTCTTTCTTTAATGGCAGTTCATATTGCGGGCCATGGCTGCCAGTCAGTGCGATAATCATAAGCTGAATTTAATTGATTTGTTGGCATGTGCGTCTATTTGATAAAATAGCTTTTTGCATTGTTTTTTTTGGAAGGAGCTTTTTGTATTGTTGGGTTGTATGCAATCCAGCCAACGAAAGCGCCCTTACGTCTCCGGTTTGGCACTGAATCCGAGATTCCCCCTAACGCGCTTTCCTCCAACTTTGGATCTTCGTTAATTTAACCCTCTGAAACTCCCCATTTTTAGAAAGGAGAGAACTTTTTAGGACAATTATATTCACCCCCACCCTAATCATTTAATCGTTGTTCTCCACTTTAAAAATATTGTATAATTTTTAAATATAAAACCTCCCACTTTTTTCTTTTAAAAGGTAAAATTATGGAAGGAAACAAGGTTAAAGGAAAAAACTTACCTCCTAATTGATGGTCAGCAGAAGATTTCTACTGACGGGTGAAAAAGTAAGTTTTATTTTTCTAATAATTAAAAAATCTTTCATTACAATGTAAACATACTCCATAAAAATTTTGCAAACTCACCTTGAATCCATTTTTTTTCTTGTCTTTTTGTTTGGTTTTGCTTGTAATAAGTTCCTTTATACATGAATCTCATCAACCCTTTCTCCAAATAGTCCAATTGACAACGAAGTGAACTTAAAAGGTGTTTCCTTTTCATACTTGCTTATCTTTCTCTTTTTGCTTATTACCCACTTTTGGGAAGAGGCCCATTGTTACTAGGATTGGGAAGATGAGAAAACATCTATCCGTTCTTAAGATATTGATTTCATCTTTGAGTAGAGAAGAAACAAACATGATTTATCTAAAATGTAGAAAGAGAATCTTCTACATAACTAAATATAGATCGGAGCAATATATTATAGAAATTCTCCTTTCACCTAAAAATTGCCCATTTTTAAATACAATTTGAATTTGTTTTCAGACATTAAGGGCCCAATTAGACAATGGCTCAATGCTCCCGCTCCCCGATGGTATCCTCATCAATGGTCGTGGGCCCAATAGAACTGCCTCTTTCAACGTGGAACAAGGTAATTACTAATTAGAGCTCCACGTAATTCTATTTTTAGTGAAAAAGTCATTGAGAGTGAAGCTGACTTCTGTTTCGTCTTTAAAGAAAAAGCTATTCAGAAAATGCCACAAACGGCAGCTCTGATCATCGGTTGTGTACATTGTGTTTTATTAGGAAAAACTTACAGGCTGAGGATCTCAAACGTGGGGCTCCAAAGCTCTCTCAACTTCCGAATTCAAGGCCACAGGTTGAAGGTGGTCGAAGTTGAAGGAACCCACACCCTCCAAACGGAATTCTCTTCCCTTGACGTTCACGTCGGCCAATCTTATTCTGTTCTTGTCACGGCGGATCAGCCCGCTCAGGACTTTTACATTGTCGTTTCTTCTCGCTTCACCACTCCGATCCTCACCACCACCGCTATTCTCCGTTATGCCAACTCCGCCGGCTCCGTCCAAGGCCCACCTCCTGGTGGCCCAACCATCCAAATTGATTGGTCCCTTAACCAAGCTCGAGCCATCAGGTAATCTACCCAACTTTAAACATTATATCAGTCACGTTAATTATCGAAAATCTAAGCGTTCAAGTTAATGGATTATGTTTTGTTTAATCTGGAAACCAAACCAATCTTTTACCTCGAAACTTAGCCAAACGTGCCCATTTTTATTTTAAATTTCAATCTCATTAATTTTAACATTAAATTTGGATAGGGGTTACCAGTTTTATCTTCGACTTCAATTTCATTAAATTTAACCATAGTTACTAAATTGATGGGACTTGAAATATGATACTAAAATTATAATTTTGTTGAAATGTGTAAGTTTTAGAAAATTTAATAGTCTATTTTGTGGATTGAGGTAACTTGATTTTAATTAATAAAACTTGTAAACATTTATGTAAGTGTAAGATTTAATTGGATGAAATTGAAAATTTAGGTCAAAAGTTTGCAAAAGTTAGAAGTATGAGATTGATTTTCTTTCTTTAATTTATGTAAATTATTAATACCAAGTTTGTTAACCAGAGAGAAAACTAACAAACTCAAGAACGCTGATAGGCTTATTGCTAATGCTTTTAAGTTTAACTTCAATAATCTCAAGAGTTTCCTTATGAATGCAGGACCAATCTTACAGCCAGTGGGCCTCGGCCGAACCCACAAGGGTCCTATCACTATGGTCTCATCAACACAACCAGAACCATCGTGCTGTCGAACTCAGCCGGCCTTGTCAACGGGAAGCAAAGATACGCCGTCAACAGTGTCTCGTTTATTCCGGCAGATACTCCCCTGAAGGTCGCCGACTTCTTCAAAATTGGAGGAGTCTTCCGTGTCGGAAGCATTTCTGACTGGCCAAACGGTGGCGGAATCTACCAAGACACCTCTGTAATGGGCGCTGATTACAGAGCTTTCATCGAAATCGTTTTCCAGAACAACGAAGACCTCATCCAGAGCTGGCACCTCGATGGCTACTCCTTCTTCGTTGTTGGGTAATGAAACTAAACATTAACCAACCTCATTTTCTGCTTCTTCTTGATTTCTCTGGTTTTATTCTTTGAAAATTTGTCAAAATTCAGCATGGACGGAGGGCAATGGACATCGAATAGCAGAAACCAATACAATCTACGAGACGCAGTTGCTCGTTGTACCACTCAGGTAGAACAAAACTCAAGTTCTTTATTTCTGTTTTGGGTTCATCAGCTTTTTGAACTGTGAGGAAGGAAAGATGAAGTTGTTTGTTGTTAATGGCAGGTGTACCCGAAGTCATGGACAGCAATATATGTGGCCTTGGACAACGTGGGAATGTGGAATTTAAGGTCAGAATTCTGGGCAAGGCAGTATCTGGGGCAACAATTTTACTTGAGAGTTTACACACCATCGACTTCGTTGAGAGATGAGTACCCAATTCCAAAGAACGCTCTGCTCTGTGGAAGAGCCGCTGGGAGACGAACGAGGCCACTCTAGGCTGGCTCTCTACCCATGAGATCAAGACAATCCTCAAGACTCAGCTGGGCGGCGAAGGCCTGCCTTGGCTTCGAAGTGTTTTGTCATATTTAGTTCTTCTGCGAAGGATATTTGAGAAGAGGTCCTCAGCTCGAGGTCATATATAATTTTTCTTTCTTATCATAGTATTTCATTGAATTTCCTTTCTTTGTTGTTCCGAAGTGATATTTTTTTTAGCTAATAAACATTCTTATATTTACGGGTTGGAAGTTATGATAAAAGGCTAAATATA

mRNA sequence

CCTACTCTCTCTGCACACTCGCTTAAATATAAAACCCCCCCTTTCTTCCTCTCTCACTCCTCCATAGCCATTTCTCTCTCTTCACCTCCATCTCCATTGCTCTCTTTCAGCAATGGCGTCTCCTTCACGCCGATCCTCTCTTCCACCTCTCCTCTGCCTCTGCATTTCCTTCTTCGCCATTGTTCGAGCCGACGATCCTTACAGATTCTTCGACTGGAACGTCACCTATGGCACCATTTACCCCCTCGGCGTCCCCCAACAGGGTATTCTAATCAATGGCCAATTTCCTGGCCCCGATATTCACTCTGTTACCAATGATAACATCATTATCAATGTCTACAATAGCTTGGATGAGCCCTTTCTCCTCTCCTGGAATGGGATTCAACAGAGAAGGAACTCGTACGAAGACGGTGTGTACGGAACAACATGCCCAATTCCTCCAGGGAAGAACTTCACTTACATTCTCCAAATGAAAGATCAGATCGGAAGCTTCTATTACTTCCCTTCTCTCGGATTCCACAAGGCCGCCGGTGGTTTTGGTGGAATCAGAATTCTCAGCCGCCCCCGGATTCCCGTTCCCTTCCCGGATCCCGCCGGCGATTACACCGTCCTCATCGGCGACTGGTACCAAGCTAATCATACGACATTAAGGGCCCAATTAGACAATGGCTCAATGCTCCCGCTCCCCGATGGTATCCTCATCAATGGTCGTGGGCCCAATAGAACTGCCTCTTTCAACGTGGAACAAGGAAAAACTTACAGGCTGAGGATCTCAAACGTGGGGCTCCAAAGCTCTCTCAACTTCCGAATTCAAGGCCACAGGTTGAAGGTGGTCGAAGTTGAAGGAACCCACACCCTCCAAACGGAATTCTCTTCCCTTGACGTTCACGTCGGCCAATCTTATTCTGTTCTTGTCACGGCGGATCAGCCCGCTCAGGACTTTTACATTGTCGTTTCTTCTCGCTTCACCACTCCGATCCTCACCACCACCGCTATTCTCCGTTATGCCAACTCCGCCGGCTCCGTCCAAGGCCCACCTCCTGGTGGCCCAACCATCCAAATTGATTGGTCCCTTAACCAAGCTCGAGCCATCAGGACCAATCTTACAGCCAGTGGGCCTCGGCCGAACCCACAAGGGTCCTATCACTATGGTCTCATCAACACAACCAGAACCATCGTGCTGTCGAACTCAGCCGGCCTTGTCAACGGGAAGCAAAGATACGCCGTCAACAGTGTCTCGTTTATTCCGGCAGATACTCCCCTGAAGGTCGCCGACTTCTTCAAAATTGGAGGAGTCTTCCGTGTCGGAAGCATTTCTGACTGGCCAAACGGTGGCGGAATCTACCAAGACACCTCTGTAATGGGCGCTGATTACAGAGCTTTCATCGAAATCGTTTTCCAGAACAACGAAGACCTCATCCAGAGCTGGCACCTCGATGGCTACTCCTTCTTCGTTGTTGGCATGGACGGAGGGCAATGGACATCGAATAGCAGAAACCAATACAATCTACGAGACGCAGTTGCTCGTTGTACCACTCAGGTGTACCCGAAGTCATGGACAGCAATATATGTGGCCTTGGACAACGTGGGAATGTGGAATTTAAGGTCAGAATTCTGGGCAAGGCAGTATCTGGGGCAACAATTTTACTTGAGAGTTTACACACCATCGACTTCGTTGAGAGATGAGTACCCAATTCCAAAGAACGCTCTGCTCTGTGGAAGAGCCGCTGGGAGACGAACGAGGCCACTCTAGGCTGGCTCTCTACCCATGAGATCAAGACAATCCTCAAGACTCAGCTGGGCGGCGAAGGCCTGCCTTGGCTTCGAAGTGTTTTGTCATATTTAGTTCTTCTGCGAAGGATATTTGAGAAGAGGTCCTCAGCTCGAGGTCATATATAATTTTTCTTTCTTATCATAGTATTTCATTGAATTTCCTTTCTTTGTTGTTCCGAAGTGATATTTTTTTTAGCTAATAAACATTCTTATATTTACGGGTTGGAAGTTATGATAAAAGGCTAAATATA

Coding sequence (CDS)

ATGGCGTCTCCTTCACGCCGATCCTCTCTTCCACCTCTCCTCTGCCTCTGCATTTCCTTCTTCGCCATTGTTCGAGCCGACGATCCTTACAGATTCTTCGACTGGAACGTCACCTATGGCACCATTTACCCCCTCGGCGTCCCCCAACAGGGTATTCTAATCAATGGCCAATTTCCTGGCCCCGATATTCACTCTGTTACCAATGATAACATCATTATCAATGTCTACAATAGCTTGGATGAGCCCTTTCTCCTCTCCTGGAATGGGATTCAACAGAGAAGGAACTCGTACGAAGACGGTGTGTACGGAACAACATGCCCAATTCCTCCAGGGAAGAACTTCACTTACATTCTCCAAATGAAAGATCAGATCGGAAGCTTCTATTACTTCCCTTCTCTCGGATTCCACAAGGCCGCCGGTGGTTTTGGTGGAATCAGAATTCTCAGCCGCCCCCGGATTCCCGTTCCCTTCCCGGATCCCGCCGGCGATTACACCGTCCTCATCGGCGACTGGTACCAAGCTAATCATACGACATTAAGGGCCCAATTAGACAATGGCTCAATGCTCCCGCTCCCCGATGGTATCCTCATCAATGGTCGTGGGCCCAATAGAACTGCCTCTTTCAACGTGGAACAAGGAAAAACTTACAGGCTGAGGATCTCAAACGTGGGGCTCCAAAGCTCTCTCAACTTCCGAATTCAAGGCCACAGGTTGAAGGTGGTCGAAGTTGAAGGAACCCACACCCTCCAAACGGAATTCTCTTCCCTTGACGTTCACGTCGGCCAATCTTATTCTGTTCTTGTCACGGCGGATCAGCCCGCTCAGGACTTTTACATTGTCGTTTCTTCTCGCTTCACCACTCCGATCCTCACCACCACCGCTATTCTCCGTTATGCCAACTCCGCCGGCTCCGTCCAAGGCCCACCTCCTGGTGGCCCAACCATCCAAATTGATTGGTCCCTTAACCAAGCTCGAGCCATCAGGACCAATCTTACAGCCAGTGGGCCTCGGCCGAACCCACAAGGGTCCTATCACTATGGTCTCATCAACACAACCAGAACCATCGTGCTGTCGAACTCAGCCGGCCTTGTCAACGGGAAGCAAAGATACGCCGTCAACAGTGTCTCGTTTATTCCGGCAGATACTCCCCTGAAGGTCGCCGACTTCTTCAAAATTGGAGGAGTCTTCCGTGTCGGAAGCATTTCTGACTGGCCAAACGGTGGCGGAATCTACCAAGACACCTCTGTAATGGGCGCTGATTACAGAGCTTTCATCGAAATCGTTTTCCAGAACAACGAAGACCTCATCCAGAGCTGGCACCTCGATGGCTACTCCTTCTTCGTTGTTGGCATGGACGGAGGGCAATGGACATCGAATAGCAGAAACCAATACAATCTACGAGACGCAGTTGCTCGTTGTACCACTCAGGTGTACCCGAAGTCATGGACAGCAATATATGTGGCCTTGGACAACGTGGGAATGTGGAATTTAAGGTCAGAATTCTGGGCAAGGCAGTATCTGGGGCAACAATTTTACTTGAGAGTTTACACACCATCGACTTCGTTGAGAGATGAGTACCCAATTCCAAAGAACGCTCTGCTCTGTGGAAGAGCCGCTGGGAGACGAACGAGGCCACTCTAG

Protein sequence

MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRPL
Homology
BLAST of Lcy01g015870 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 8.2e-174
Identity = 293/535 (54.77%), Postives = 377/535 (70.47%), Query Frame = 0

Query: 10  LPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTND 69
           L   L L  +   +V A+DPY    WNVTYGT  PLGVPQQ ILINGQFPGP+I+S +N+
Sbjct: 7   LAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNN 66

Query: 70  NIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYY 129
           N+IINV+N+LDEPFLL+WNGIQ R+N ++DG  GT CPI PG N+TY  Q KDQIGS++Y
Sbjct: 67  NVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFY 126

Query: 130 FPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSML 189
           +P+ G H+AAGG+GG+R+ SR  IPVP+ DP  DYTVLIGDWY  +HT L+  LD G  +
Sbjct: 127 YPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTI 186

Query: 190 PLPDGILINGRGPNRTAS----FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEG 249
             PDGI+ING+      S    F ++ GKTYR+RI NVG+++S+NFRIQ H++K+VE+EG
Sbjct: 187 GRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEG 246

Query: 250 THTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSV 309
           +H LQ ++ SLDVHVGQ +  +VTA+Q  +D+Y+V SSRF   ++TTT +LRY    G  
Sbjct: 247 SHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPA 306

Query: 310 QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVN 369
               P GP +   WSLNQ R+ R NLTAS  RPNPQGSYHYG IN TRTI L N+ G V+
Sbjct: 307 SSQLPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 366

Query: 370 GKQRYAVNSVSFIPADTPLKVADFFKIGG-VFRVGSISDWPNGGGIYQ---DTSVMGADY 429
           GK R+A+N VS    +TPLK+A++F I   VF+  +I+D P    I     + +V+   +
Sbjct: 367 GKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITH 426

Query: 430 RAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKS 489
           R F+E+VF+N+E  +QSWHLDGYSFF V ++ G WT   R  YNL DAV+R T QVYPK 
Sbjct: 427 RTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 486

Query: 490 WTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 537
           W AI +  DN GMWN+RSE   R+YLGQQ Y  V +P  SLRDEY +P+ +L CG
Sbjct: 487 WAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of Lcy01g015870 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.6e-169
Identity = 287/538 (53.35%), Postives = 369/538 (68.59%), Query Frame = 0

Query: 13  LLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNII 72
           LLCL +     V A+DPY +F+WNVTYGTI PLGVPQQGILINGQFPGP I+  +N+NI+
Sbjct: 12  LLCLSVG----VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIV 71

Query: 73  INVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 132
           +NV+N+LDEPFL +WNG+Q R+NS++DG  GT CPI PG+NFTY  Q+KDQIGS+ YFP+
Sbjct: 72  VNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPT 131

Query: 133 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 192
              H+AAGG+G + + SR  IPVPF +PA +Y V +GDWY   H TL+  LD G  +  P
Sbjct: 132 TALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRP 191

Query: 193 DGILINGR----GPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHT 252
           DGI+ING+    G  +   F +E GKTYR R  N+G++SS+N R QGH +K+VE+EG+HT
Sbjct: 192 DGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHT 251

Query: 253 LQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQ-- 312
           +Q  + SLD+HVGQ  SVLVTADQ  +D+Y+VVSSRF    L++ AI+RYAN  G     
Sbjct: 252 VQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPE 311

Query: 313 -GPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVN 372
              PP   T  I WS+NQ R+ R NLTAS  RPNPQGSYHYG IN TRTI + NS   V 
Sbjct: 312 LPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVG 371

Query: 373 GKQRYAVNSVSFIPADTPLKVADFF-KIGGVFRVGSISDW--PNGGGIYQDTSVMGADYR 432
           GK RY +N +S    +TPLK+ ++F      F+   ++D    +   +   T+V  A YR
Sbjct: 372 GKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYR 431

Query: 433 AFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSW 492
            F+EI+F+N+E  I+++HLDGYSFF V ++ G+W+   R  YNL D ++R   QVYP SW
Sbjct: 432 NFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSW 491

Query: 493 TAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 541
            AI +  DN GMWNLRSE W + YLG+Q Y  V +PS SLRDEY IP N  LCG   G
Sbjct: 492 AAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 545

BLAST of Lcy01g015870 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 2.2e-155
Identity = 276/530 (52.08%), Postives = 355/530 (66.98%), Query Frame = 0

Query: 26  ADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNIIINVYNSLDEPFLL 85
           A DPY F+++ V+Y T  PLGVPQQ I ING+FPGP I+  TN+N+++NV N LDE  LL
Sbjct: 20  AADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNKLDEGLLL 79

Query: 86  SWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAGGFGGI 145
            WNGIQQRR S++DGV GT CPIPP  N+TY  Q+KDQIGSF+YFPSL F +A+GGFG  
Sbjct: 80  HWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRASGGFGSF 139

Query: 146 RILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGRGPNR- 205
            +  R  IPVPF  P GD TV IGDWY  NHT LR  LD+G  L +PDG+LING+GP R 
Sbjct: 140 VVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLINGKGPYRY 199

Query: 206 ----------TASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQTEFS 265
                       +  V  GKTYRLR+SNVG+ +SLNFRIQGH L + E EG++T+Q  ++
Sbjct: 200 NDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYT 259

Query: 266 SLDVHVGQSYSVLVTADQPA-QDFYIVVSSRFTTPIL----TTTAILRYANSAGSVQGPP 325
           SLD+HVGQSYS LVT DQ A  D+YIV S+R     +    T   IL+Y NS G  +G  
Sbjct: 260 SLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQL 319

Query: 326 PGGPTIQID--WSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGL-VNG 385
           P GP  + D  +S+NQAR+IR N++ASG RPNPQGS+ YG IN T   VL N   + ++G
Sbjct: 320 PPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISG 379

Query: 386 KQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIE 445
           K+R  +N +SF    TP+++AD  K+  V+++      P  G     TS++   YR F+E
Sbjct: 380 KRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPKRPLTGPAKVATSIINGTYRGFME 439

Query: 446 IVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIY 505
           +V QNN+  +QS+H+ GY+FFVVGMD G+WT NSR  YN  D +AR T QVYP +W+AI 
Sbjct: 440 VVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAWSAIL 499

Query: 506 VALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 537
           ++LDN G WNLR+E     YLGQ+ Y+RV  P  + + E+  P N L CG
Sbjct: 500 ISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCG 548

BLAST of Lcy01g015870 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.5e-154
Identity = 274/542 (50.55%), Postives = 361/542 (66.61%), Query Frame = 0

Query: 13  LLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNII 72
           LLC  +   A+  A DP+  +D+ V+Y T  PLGVPQQ I +NGQFPGP +++ TN N++
Sbjct: 12  LLCFAL-LSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVV 71

Query: 73  INVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 132
           +NV+N LDEP LL+W GIQ RRNS++DGV GT CPIPP  NFTY  Q+KDQIGSF+Y PS
Sbjct: 72  VNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPS 131

Query: 133 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 192
           L F +A+GGFG I I +R  IP+PFP P G+   +IGDWY  +H  LR  LD+G  L +P
Sbjct: 132 LNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMP 191

Query: 193 DGILINGRGPNRTAS----------FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVE 252
           DG+LING+GP +  S          F+VE GKTYR+R+ NVG+ +SLNFRIQ H L +VE
Sbjct: 192 DGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVE 251

Query: 253 VEGTHTLQTEFSSLDVHVGQSYSVLVTADQPA-QDFYIVVSSRFTTPI----LTTTAILR 312
            EG +T Q  F+  DVHVGQSYS LVT DQ A  D+YIV S+RF        +T  AIL 
Sbjct: 252 TEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILH 311

Query: 313 YANSAGSVQGPPPGGPT-IQIDWS-LNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTI 372
           Y+NS G V GP P   T +   WS ++Q + IR N +ASG RPNPQGS+HYG IN T T 
Sbjct: 312 YSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY 371

Query: 373 VL-SNSAGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDT 432
           +L S    ++NG  R  +N +SF+   TP+++AD  K+ G +++    D P    +  D 
Sbjct: 372 ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRPLRLDR 431

Query: 433 SVMGADYRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCT 492
           S++ A Y+ FI++VFQNN+  IQS+H+DGYSFFVVGMD G W+ + +  YN  DA++R T
Sbjct: 432 SMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRST 491

Query: 493 TQVYPKSWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALL 537
            +VYP  WTA+ ++LDNVG+WN+R E   R YLG++ Y+R+  P    + E   P N L 
Sbjct: 492 IEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLY 551

BLAST of Lcy01g015870 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.7e-151
Identity = 265/535 (49.53%), Postives = 356/535 (66.54%), Query Frame = 0

Query: 21  FAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNIIINVYNSLD 80
           F    A DPY  +D+ ++Y T  PLGVPQQ I +NG+FPGP I++ TN N+ +NV N LD
Sbjct: 18  FGFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLD 77

Query: 81  EPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAG 140
           EP LL+W G+Q RRNS++DGV GT CPIPP  NFTY  Q+KDQIGS++Y PSL F +A+G
Sbjct: 78  EPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASG 137

Query: 141 GFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGR 200
           GFG + I +R  +P+PF +P G+   +IGDWY  NHT LR  LD+G  L +PDG+LING+
Sbjct: 138 GFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGK 197

Query: 201 GPNRTAS----------FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQ 260
           GP +  S           NV+ GKTYR+R+ NVG+ +SLNFRIQ H+L ++E EG +T Q
Sbjct: 198 GPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQ 257

Query: 261 TEFSSLDVHVGQSYSVLVTADQPA-QDFYIVVSSRFTTPI----LTTTAILRYANSAGSV 320
             F+  DVHVGQSYS LVT DQ A  D+YIV S+RF        +T   IL Y+NS G  
Sbjct: 258 MNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPA 317

Query: 321 QGPPPGGPT-IQIDWS-LNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVL-SNSAG 380
            GP P   T +   WS +NQ RAI+ N +ASG RPNPQGS+HYG IN TRT +L S    
Sbjct: 318 SGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPT 377

Query: 381 LVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQ-DTSVMGADY 440
            +NGK R  +N +SF+   TP+++AD  K+ G + +    D P    + +  +S++ A Y
Sbjct: 378 KINGKLRATLNGISFVNPSTPMRLADDHKVKGDYML-DFPDRPLDEKLPRLSSSIINATY 437

Query: 441 RAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKS 500
           + FI+++FQNN+  IQS+H+DGY+F+VV MD G W+ +  + YN  DAVAR T +VYP +
Sbjct: 438 KGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGA 497

Query: 501 WTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 537
           WTA+ ++LDNVG+WN+R E   R YLGQ+ Y+R+  P  +   E   P+N + CG
Sbjct: 498 WTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of Lcy01g015870 vs. ExPASy TrEMBL
Match: A0A6J1KDJ5 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111492278 PE=3 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 529/546 (96.89%), Postives = 538/546 (98.53%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA  S RSSLPPLLCLCISFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVL+GDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLVGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTT+TIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNG GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. ExPASy TrEMBL
Match: A0A6J1HDN3 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111462565 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 528/546 (96.70%), Postives = 537/546 (98.35%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA  S RSSLPPLLCLCISFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLI TT+TIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLITTTKTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNG GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. ExPASy TrEMBL
Match: A0A6J1CRH9 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111014083 PE=3 SV=1)

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 514/535 (96.07%), Postives = 529/535 (98.88%), Query Frame = 0

Query: 12  PLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNI 71
           PLLCLC+SFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPGPDIHSVTNDN+
Sbjct: 7   PLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNL 66

Query: 72  IINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFP 131
           IINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQIGSFYYFP
Sbjct: 67  IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFP 126

Query: 132 SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPL 191
           SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNG+MLPL
Sbjct: 127 SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPL 186

Query: 192 PDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQT 251
           PDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKVVEVEGTHTLQT
Sbjct: 187 PDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQT 246

Query: 252 EFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPG 311
           EFSSLD+HVGQ YSVLVTADQPAQDFY+VVSSRFTTPILTTTAILRYANSAGSVQGPPPG
Sbjct: 247 EFSSLDIHVGQFYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPG 306

Query: 312 GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQRYA 371
           GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTT+TIVLSNSAG VNGKQRYA
Sbjct: 307 GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYA 366

Query: 372 VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEIVFQN 431
           VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP GGGIYQDTSVMGADYRA++EIVFQN
Sbjct: 367 VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQN 426

Query: 432 NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYVALDN 491
           NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPKSWTAIYVALDN
Sbjct: 427 NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDN 486

Query: 492 VGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRPL 547
           VGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA GRRTRPL
Sbjct: 487 VGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 541

BLAST of Lcy01g015870 vs. ExPASy TrEMBL
Match: A0A1S3BCD0 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103488372 PE=3 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 517/534 (96.82%), Postives = 527/534 (98.69%), Query Frame = 0

Query: 13  LLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNII 72
           LL L ISFFAIV A+DPYRFFDWNVTYGTIYPLG+PQQGILINGQFPGPDIHSVTNDNII
Sbjct: 5   LLFLFISFFAIVGAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNII 64

Query: 73  INVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 132
           INV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS
Sbjct: 65  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 124

Query: 133 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 192
           LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP
Sbjct: 125 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 184

Query: 193 DGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQTE 252
           DGILINGRGPNRTAS NVEQGKTYRLRISNVGLQSSLNFRIQGHR+KVVEVEGTHTLQTE
Sbjct: 185 DGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTE 244

Query: 253 FSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG 312
           FSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG
Sbjct: 245 FSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG 304

Query: 313 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQRYAV 372
           PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYG+INTTRTIVLSNSAG+VNGKQRYAV
Sbjct: 305 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMINTTRTIVLSNSAGIVNGKQRYAV 364

Query: 373 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEIVFQNN 432
           NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAF+EIVFQNN
Sbjct: 365 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNN 424

Query: 433 EDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYVALDNV 492
           EDLIQSWH DGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCT QVYPKSW+AIYVALDNV
Sbjct: 425 EDLIQSWHFDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNV 484

Query: 493 GMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRPL 547
           GMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA GRRTRPL
Sbjct: 485 GMWNIRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 538

BLAST of Lcy01g015870 vs. ExPASy TrEMBL
Match: A0A0A0LN97 (Multicopper oxidase OS=Cucumis sativus OX=3659 GN=Csa_2G348200 PE=3 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 512/535 (95.70%), Postives = 528/535 (98.69%), Query Frame = 0

Query: 13  LLCLCIS-FFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNI 72
           LL L IS FFAIVRA+DPYRFFDWNVTYGTIYPLG+PQQGILINGQFPGPDIHSVTNDNI
Sbjct: 5   LLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNI 64

Query: 73  IINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFP 132
           IINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFP
Sbjct: 65  IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFP 124

Query: 133 SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPL 192
           SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPL
Sbjct: 125 SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPL 184

Query: 193 PDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQT 252
           PDGILINGRGPNRTAS NVEQGKTYRLRISNVGLQSSLNFRIQGHR+KVVEVEGTHTLQT
Sbjct: 185 PDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQT 244

Query: 253 EFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPG 312
           EFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPIL+TTAILRYANSAGSVQGPPPG
Sbjct: 245 EFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPG 304

Query: 313 GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQRYA 372
           GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYG+I TTRTIV++NSAG+V+GKQRYA
Sbjct: 305 GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYA 364

Query: 373 VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEIVFQN 432
           VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAF+EIVFQN
Sbjct: 365 VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQN 424

Query: 433 NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYVALDN 492
           NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCT QVYPKSW+AIYVALDN
Sbjct: 425 NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDN 484

Query: 493 VGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRPL 547
           VGMWN+R+EFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA GRRTRPL
Sbjct: 485 VGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 539

BLAST of Lcy01g015870 vs. NCBI nr
Match: XP_023547180.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 531/546 (97.25%), Postives = 539/546 (98.72%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA  S RSSLPPLLCLCISFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTT+TIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNG GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. NCBI nr
Match: XP_038886385.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 532/546 (97.44%), Postives = 538/546 (98.53%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA     SSLPPLL LCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG
Sbjct: 1   MAIALMGSSLPPLLFLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHR+KV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRMKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTHTLQTEFSSLD+HVGQSYSVLVT DQPAQDFYIVVSSRFTTPILTTTAILRYAN
Sbjct: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTTDQPAQDFYIVVSSRFTTPILTTTAILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLI+TTRTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLISTTRTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. NCBI nr
Match: XP_022997333.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 529/546 (96.89%), Postives = 538/546 (98.53%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA  S RSSLPPLLCLCISFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVL+GDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLVGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTT+TIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNG GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. NCBI nr
Match: KAG6598531.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 529/546 (96.89%), Postives = 539/546 (98.72%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA  S RSSLPPLLCLCISFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTT+TIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSIS+WPNG GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISEWPNGAGIYEDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. NCBI nr
Match: XP_022961945.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 528/546 (96.70%), Postives = 537/546 (98.35%), Query Frame = 0

Query: 1   MASPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPG 60
           MA  S RSSLPPLLCLCISFFAIV ADDPYRFF+WNVTYGTIYPLG+PQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120
           PDIHSVTNDNIIINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240
           AQLDNGSMLPLPDGILINGRGPNRTA FNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLI TT+TIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLITTTKTIVLSNS 360

Query: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGAD 420
           AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNG GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPK 480
           YRAF+EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA+ARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAG 540
           SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRA G
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 547
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of Lcy01g015870 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 937.2 bits (2421), Expect = 6.0e-273
Identity = 444/539 (82.37%), Postives = 483/539 (89.61%), Query Frame = 0

Query: 8   SSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVT 67
           +S    L + +S    V A+DPYRFF+WN+TYG IYPLGV QQGILING FPGPDIHSVT
Sbjct: 5   ASFAAALFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVT 64

Query: 68  NDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 127
           NDN+IINVYNSLDEPFLLSWNGIQQRRNS+ DGVYGTTCPIPPGKN+TYILQMKDQIGSF
Sbjct: 65  NDNLIINVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSF 124

Query: 128 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 187
           YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGD TVLIGDWY+ANHT LRAQLDNG 
Sbjct: 125 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGK 184

Query: 188 MLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTH 247
            LPLPDGILINGR     A+ NVEQGKTYR RISNVGLQ SLNFRIQ H++KVVEVEGTH
Sbjct: 185 KLPLPDGILINGRSSG--ATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTH 244

Query: 248 TLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQG 307
           TLQT FSSLDVHVGQSYSVLVTADQ  +D+Y+VVSSRFT+ +LTTT I RY+NSAG V G
Sbjct: 245 TLQTTFSSLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSG 304

Query: 308 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGK 367
           P PGGPTIQIDWSLNQARAIRTNL+ASGPRPNPQGSYHYG+INTTRTI L++SAG V+GK
Sbjct: 305 PIPGGPTIQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGK 364

Query: 368 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEI 427
           QRYAVNSVSF PADTPLK+AD+FKI GV+R GSI   P GGGIY DTSVM  DYR F+EI
Sbjct: 365 QRYAVNSVSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEI 424

Query: 428 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYV 487
           +F+N+ED++QSWHLDGYSF+VVGMDGGQW+ +SRN+YNLRDAVARCT QVYP SWTAI +
Sbjct: 425 IFENSEDIVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILI 484

Query: 488 ALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRPL 547
           ALDNVGMWNLRSEFWARQYLGQQ YLRVYTPSTSLRDEYPIPKNALLCGRA+GR TRPL
Sbjct: 485 ALDNVGMWNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGRSTRPL 541

BLAST of Lcy01g015870 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 897.1 bits (2317), Expect = 6.9e-261
Identity = 421/534 (78.84%), Postives = 468/534 (87.64%), Query Frame = 0

Query: 13  LLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNII 72
           +L  C+SFFA V A+ PYRFFDWNVTYG IYPLGV QQGILINGQFPGPDIHSVTNDN+I
Sbjct: 11  ILLFCLSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLI 70

Query: 73  INVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 132
           INV+NSLDEPFL+SWNG+Q RRNSY DG+YGTTCPIPP  N+TYILQ+KDQIGSFYYFPS
Sbjct: 71  INVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFPS 130

Query: 133 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 192
           L FHKAAGGFGGIRILSRP IPVPF DPAGDYTVLIGDWY+ NHT L+++LD G  LP P
Sbjct: 131 LAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSP 190

Query: 193 DGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQTE 252
           DGILINGR  +  A+ NVEQGKTYRLRISNVGLQ SLNFRIQ HR+K+VEVEGTHTLQT 
Sbjct: 191 DGILINGR--SNGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTM 250

Query: 253 FSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG 312
           FSSLDVHVGQSYSVL+TADQ  +D+Y+VVSSRFT  I+TTT +LRY+ S+    GP PGG
Sbjct: 251 FSSLDVHVGQSYSVLITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSSTPASGPIPGG 310

Query: 313 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQRYAV 372
           PTIQ+DWSLNQARAIRTNLTASGPRPNPQGSYHYGLI   RTIV  +SAG +NGKQRY V
Sbjct: 311 PTIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRYGV 370

Query: 373 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEIVFQNN 432
           NSVSF+PADTPLK+ADFFKI GV+++ SISD P  GG+Y DTSV+  DYR FIEIVF+N 
Sbjct: 371 NSVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFENQ 430

Query: 433 EDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYVALDNV 492
           ED++QS+HL+GYSF+VVGMDGGQW + SRN YNLRDAV+R T QVYPKSWTAIY+ALDNV
Sbjct: 431 EDIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALDNV 490

Query: 493 GMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRPL 547
           GMWNLRSEFWARQYLGQQ YLRV+T STSLRDEYPIPKN+ LCGRA GR TRPL
Sbjct: 491 GMWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRARGRHTRPL 542

BLAST of Lcy01g015870 vs. TAIR 10
Match: AT1G21850.1 (SKU5 similar 8 )

HSP 1 Score: 839.0 bits (2166), Expect = 2.2e-243
Identity = 397/526 (75.48%), Postives = 449/526 (85.36%), Query Frame = 0

Query: 20  FFAI--VRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNIIINVYN 79
           FF I  V A+DPY+FF+W+VTYG I PL V QQGILING+FPGPDI +VTNDN+IINV+N
Sbjct: 16  FFLISFVAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTNDNLIINVFN 75

Query: 80  SLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHK 139
            LDEPFL+SW+GI+  RNSY+DGVYGTTCPIPPGKN+TY LQ+KDQIGSFYYFPSLGFHK
Sbjct: 76  HLDEPFLISWSGIRNWRNSYQDGVYGTTCPIPPGKNYTYALQVKDQIGSFYYFPSLGFHK 135

Query: 140 AAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILI 199
           AAGGFG IRI SRPRIPVPFP PAGDYTVLIGDWY+ NH  LRAQLDNG  LP PDGILI
Sbjct: 136 AAGGFGAIRISSRPRIPVPFPAPAGDYTVLIGDWYKTNHKDLRAQLDNGGKLPFPDGILI 195

Query: 200 NGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQTEFSSLD 259
           NGRG    A+ N+E GKTYRLRISNVGLQ+SLNFRIQ H++K+VEVEGTHT+QT FSSLD
Sbjct: 196 NGRGSG--ATLNIEPGKTYRLRISNVGLQNSLNFRIQNHKMKLVEVEGTHTIQTPFSSLD 255

Query: 260 VHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGGPTIQI 319
           VHVGQSYSVL+TADQPA+D+YIVVSSRFT+ IL T  +L Y+NSAG V GP P  P IQ+
Sbjct: 256 VHVGQSYSVLITADQPAKDYYIVVSSRFTSKILITAGVLHYSNSAGPVSGPIPEAP-IQL 315

Query: 320 DWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQRYAVNSVSF 379
            WS +QARAI+TNL ASGPRPNPQG+YHYG I  TRTI L++SAG +NGKQRYAVNS SF
Sbjct: 316 RWSFDQARAIKTNLAASGPRPNPQGTYHYGKIKVTRTIKLASSAGNINGKQRYAVNSASF 375

Query: 380 IPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFIEIVFQNNEDLIQ 439
            P DTPLK+AD+FKI GV+  GSI D P  G IY  TSVM  DY+AF+EIVF+N ED++Q
Sbjct: 376 YPTDTPLKLADYFKIAGVYNPGSIPDQPTHGAIYPVTSVMQTDYKAFVEIVFENWEDIVQ 435

Query: 440 SWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYVALDNVGMWNL 499
           +WHLDGYSFFVVGM+ G+W++ SR  YNL DAV+RCT QVYP+SWTAIYV+LDNVGMWNL
Sbjct: 436 TWHLDGYSFFVVGMELGKWSAASRKVYNLNDAVSRCTVQVYPRSWTAIYVSLDNVGMWNL 495

Query: 500 RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRT 544
           RSE W RQYLGQQFY+RVYTPSTSLRDEY IPKNALLCGRA G  T
Sbjct: 496 RSELWERQYLGQQFYMRVYTPSTSLRDEYLIPKNALLCGRATGHHT 538

BLAST of Lcy01g015870 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 822.0 bits (2122), Expect = 2.8e-238
Identity = 386/537 (71.88%), Postives = 451/537 (83.99%), Query Frame = 0

Query: 3   SPSRRSSLPPLLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPD 62
           S + R+ +  LL L     ++  A+DPYRFF+W+VTYG I PLGV QQGILING+FPGPD
Sbjct: 5   SMNTRAMITTLLFL----ISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPD 64

Query: 63  IHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKD 122
           I S+TNDN+IINV+N LDEPFLLSWNGI+  +NS++DGVYGT CPIPPGKN+TY LQ+KD
Sbjct: 65  IISITNDNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKD 124

Query: 123 QIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQ 182
           QIGSFYYFPSLGFHKAAGGFGGIRI SR  IPVPFP PA DYT+L+GDWY+ NH  L+AQ
Sbjct: 125 QIGSFYYFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQ 184

Query: 183 LDNGSMLPLPDGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVE 242
           LDNG  LPLPDGILINGR     A+ N+E GKTYRLRISNVGLQ+SLNFRIQ H +K+VE
Sbjct: 185 LDNGGKLPLPDGILINGRSSG--ATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVE 244

Query: 243 VEGTHTLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSA 302
           VEG +T+Q  FSSLDVHVGQSYSVL+TADQPA+D+Y+VVSSRFT+ ILTTT +L Y+NS 
Sbjct: 245 VEGRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSV 304

Query: 303 GSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAG 362
             V GP P GP I++ WS NQARAIRTNLTASGPRPNPQGSY YG+IN TRTI L+N+ G
Sbjct: 305 APVSGPIPDGP-IKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLG 364

Query: 363 LVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYR 422
            + GKQRYAVNS SF PADTPLK+ D+FKI GV++ GSISD P  G I+  TSVM AD+R
Sbjct: 365 HIEGKQRYAVNSASFYPADTPLKLVDYFKIDGVYKPGSISDQPTNGAIFPTTSVMQADFR 424

Query: 423 AFIEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSW 482
           AF+E++F+N+ED++QSWHLDGYSF+VVGM+ G+W+  SR  YNL DA+ RCT QVYP+SW
Sbjct: 425 AFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSW 484

Query: 483 TAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAA 540
           TAIY+ALDNVGMWN+RSE W RQYLGQQFY+RVYT STSLRDEY IPKNALLCGRA+
Sbjct: 485 TAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCGRAS 534

BLAST of Lcy01g015870 vs. TAIR 10
Match: AT4G38420.1 (SKU5 similar 9 )

HSP 1 Score: 792.0 bits (2044), Expect = 3.1e-229
Identity = 365/538 (67.84%), Postives = 446/538 (82.90%), Query Frame = 0

Query: 13  LLCLCISFFAIVRADDPYRFFDWNVTYGTIYPLGVPQQGILINGQFPGPDIHSVTNDNII 72
           ++ + IS  + V+ADDPYRFFDW VTYG I PLG+PQ+GILINGQ+PGPDI+SVTNDN+I
Sbjct: 12  MMMMTISIISFVQADDPYRFFDWRVTYGNISPLGIPQRGILINGQYPGPDIYSVTNDNLI 71

Query: 73  INVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 132
           INV+N LDEPFLLSWNG+Q R+NSY+DGVYGTTCPIPPGKN+TY +Q+KDQIGSF+YFPS
Sbjct: 72  INVHNDLDEPFLLSWNGVQLRKNSYQDGVYGTTCPIPPGKNYTYAIQVKDQIGSFFYFPS 131

Query: 133 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 192
           L  HKAAGGFGG RILSRPRIPVPFP+PAGD+T LIGDW++ +H  L+A LD G  LPLP
Sbjct: 132 LAVHKAAGGFGGFRILSRPRIPVPFPEPAGDFTFLIGDWFKHDHKVLKAILDRGHKLPLP 191

Query: 193 DGILINGRGPNRTASFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKVVEVEGTHTLQTE 252
            G+LING+G +  +S  V +GKTYR RISNVGLQ +LNFRIQGH++K+VEVEGTHT+Q+ 
Sbjct: 192 QGVLINGQGVSYMSSITVHKGKTYRFRISNVGLQHTLNFRIQGHQMKLVEVEGTHTVQSM 251

Query: 253 FSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANS----AGSVQGP 312
           ++SLD+HVGQSYSVLVT DQP QD+ IVVS++F    L  ++ + Y+NS    + S    
Sbjct: 252 YTSLDIHVGQSYSVLVTMDQPDQDYDIVVSTKFVAKKLLVSSTIHYSNSRHSHSSSANSV 311

Query: 313 PPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTRTIVLSNSAGLVNGKQ 372
               P  ++DWS+ QAR+IRTNLTASGPRPNPQGSYHYG I  +RT++L +SA LV  KQ
Sbjct: 312 HVQQPADELDWSIKQARSIRTNLTASGPRPNPQGSYHYGRIKISRTLILESSAALVKRKQ 371

Query: 373 RYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP-NGGGIYQDTSVMGADYRAFIEI 432
           RYA+N VSF+P DTPLK+AD+FKI GVF++GSI D P  G G+  +TSVMGA +R F+EI
Sbjct: 372 RYAINGVSFVPGDTPLKLADYFKIKGVFKMGSIPDKPRRGRGMRMETSVMGAHHRDFLEI 431

Query: 433 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAVARCTTQVYPKSWTAIYV 492
           +FQN E ++QS+HLDGYSF+VVG D G W+  SR +YNLRDA++R TTQVYP+SWTA+YV
Sbjct: 432 IFQNREKIVQSYHLDGYSFWVVGTDRGTWSKASRREYNLRDAISRSTTQVYPESWTAVYV 491

Query: 493 ALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAAGRRTRP 546
           ALDNVGMWNLRSE+WARQYLGQQFYLRVY+P+ SLRDEY +PKNALLCGRA+ + T P
Sbjct: 492 ALDNVGMWNLRSEYWARQYLGQQFYLRVYSPTHSLRDEYLLPKNALLCGRASNKHTTP 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006248.2e-17454.77L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291621.6e-16953.35L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU402.2e-15552.08Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX52.5e-15450.55Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN65.7e-15149.53Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1KDJ50.0e+0096.89L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111492278 PE=3 SV=... [more]
A0A6J1HDN30.0e+0096.70L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111462565 PE=3 S... [more]
A0A6J1CRH90.0e+0096.07L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111014083 PE=3 ... [more]
A0A1S3BCD00.0e+0096.82L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103488372 PE=3 SV=1[more]
A0A0A0LN970.0e+0095.70Multicopper oxidase OS=Cucumis sativus OX=3659 GN=Csa_2G348200 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023547180.10.0e+0097.25L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo][more]
XP_038886385.10.0e+0097.44L-ascorbate oxidase homolog [Benincasa hispida][more]
XP_022997333.10.0e+0096.89L-ascorbate oxidase homolog [Cucurbita maxima][more]
KAG6598531.10.0e+0096.89L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022961945.10.0e+0096.70L-ascorbate oxidase homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G76160.16.0e-27382.37SKU5 similar 5 [more]
AT1G41830.16.9e-26178.84SKU5-similar 6 [more]
AT1G21850.12.2e-24375.48SKU5 similar 8 [more]
AT1G21860.12.8e-23871.88SKU5 similar 7 [more]
AT4G38420.13.1e-22967.84SKU5 similar 9 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 161..337
e-value: 1.4E-41
score: 144.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 30..151
e-value: 1.6E-29
score: 104.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 347..542
e-value: 8.0E-34
score: 119.1
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 26..177
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 157..338
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 348..537
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 163..300
e-value: 2.1E-42
score: 144.9
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 383..518
e-value: 2.4E-28
score: 98.6
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 37..150
e-value: 1.6E-36
score: 124.9
NoneNo IPR availablePANTHERPTHR11709:SF387OSJNBA0084K11.18 PROTEINcoord: 15..539
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 15..539
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 162..298
e-value: 2.12136E-79
score: 243.464
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 32..147
e-value: 1.11109E-74
score: 230.757
IPR034275Ascorbate oxidase homologue, third cupredoxin domainCDDcd13894CuRO_3_AAO_like_1coord: 379..500
e-value: 1.03131E-69
score: 218.068

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g015870.1Lcy01g015870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity