Lcy01g006150 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g006150
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCOP9 signalosome complex subunit 1-like
LocationChr01: 8780387 .. 8783190 (-)
RNA-Seq ExpressionLcy01g006150
SyntenyLcy01g006150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTTCAGGAGAAGCGAATTCGTTGACGACGCCGGAGAATCGGAAGATAATTATCGTAGTACCTCCGGCAGCGACGAAGAAGAAAAACTTAACTCTCCGACGCCGTTCTTGAAGGACAAGCTTATCGACATCCATGCCTACGCGAGCAACTACCGCCGTCGAATGAAAATCCTCCGCCTCGTATTCATCGCCGACCGCTTCGGCGACAGTAATGAACGGCGGCGGATGACTCAACTAGGAGCACTCCAAATCGCCTACGACGAGGCCAAGAAGGGCAAGGACGTCGCTCTCTTCACCAAAATCGCCGAGAAAATTAGGGGAAGATTGGGCCCTAATTACGCCATGGATGAAAATTGGCGCAATTCGGTGAATCAAGAGAAGCAAGTAATCAGCCAAATGCTACAGAGATGCACGAAGAATTCCGAGGTATGAATTTAATCAATCGATCGATCGATCGATCGATCATTGAATCAAATTCTCTGTTCTTGAGCAATCTTGATATACGTTCGTGGTTTCGATCGCGATAGATTTGTTACGCGAACAGAATCATTTCTGCGGACGACGCAAGGATTTTATTCAATGCGGTTGCGGATTTCCACCACATCCATGGCGCACCTGCAACAGCTTTTGAATTTTACAAGAAGAGCGTACGTTTCTGCAGGACCGATGAGGATCTCTCTGAATTTTTATCGCGAGTGCTTTTGACGGTGATCGATCTTGGCCAGTTCGAACACGTTGCGAAGATGGCAGAAAAAGGAATTCAAAATCCGAAGCTTAATCCGATGACTACTGACAGTCTGCGCTGCGCTCTTGGATTGGCTCGCTTCAAGGCACAACGCTACAAAGATGCTGCTCATCAGGTTTGAATTAATTAATCCATTCATTCCATAAAATTCAATATTAATTATGTTTACATTGGCAATGTGTGCAGTTCTTGGGAATCAGTAAAGAATTGGGAAACCGATTTTCGGATGTGATGGCTGCTCATGATATTGCAGCCTTTGGTGCGCTATGTGCACTTGCAACTTTTGAACCACTGGAGTTGAAGGCAACAATTTCTTTTGTTCTTGTTTTAATCTTTTTCTTTTTCTTTTTCTCCAATTACTTTTGCATAAATTTTTTGATCTGTTTACTGGTTTTTGACATTGGCAGACTTTAGTTATGGATAATGAGAATTTTCAGTTCTATTTGGATTCGGCTGTTGAAGTCAAGGAGCTCATCAAGGATTTTTGTACCAGGTATTGTAGTGAACATTAATTATCGTTTTTTAAACATCTACACCCTTTTTGTGTCTAATAATTAGTCTCTTGTTTATGAAAAGTTATGGGAAAAATTGTAGTTTTATTTCCCAAATTTCCTCGAGTGTCTATTTGATCATTAAAGTTTTTAGAAGTTTAGATTTAGCCTTTAAAGTGTTCGACATTGCTATGTTGAGCTATAAGGATCTGTTTGGAATAATACATTTTAATTTAAATGTTTTTCAAGAATACATTTTTTACAAAACATTTTGCATAAAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTGCATTTTCTCATAAGTGTTTTTATACACAAGCTTAAAAGTCTTTCTTAAAATGGTTTATTTATCAATCTCATTTTCTAATGAGTCATTTTTAGTAGTTGTCAAACACTAGAAATTTTTTCAAAATGATTTATTTTCAAAATTAAACACTTGAAAAGTTAAACCAAACACACCCTAATGGTAGTTGATTGGGGAGGGAAAAAGAAAATTGTTTTTTTTTTCTGATATTTAAATGACAAGTCAACCATTATAATAATTTAATATATAAGTTTTGACACATCAGCTTTATAAATTATAAATTAATTAAAAAAAACTATAGCATTTAGTTGCTGTTACTGCAGTTAATAATATAAATTATGGAAAAGTTCTTTCCAAACAATTTTTTCAAAATTGGACCGCTAAATTGTAACTTTTAAAACTTTATAGACCAAATAGACACATGGTAAAAACTCAATTTTCCTAAAGTTTTAATTATGTTTTTTTTTTTTTTTTTTTTTTGAAAAAAAGTTTTAGTTATGTTTTTTAATTTTGGATTATATTTCTATTTAATTATTGTCACGCATTAACAGTGCTAAAGGTAGAGATGGAATTTTTTTAAAAAAAAGGTATAATTCAAAGTTTAAAATTAAATCATTTGAAAGGATATAGAAGTAATTGAAACTTTTTTGAAGTTGATGAGGCTAATTGAATTTAACTTACTTTTGCTAGGCGTTATTCTTCATTCCTCAAACACTTTGATAACATCAAAGGATGGCTGTTGCTTGACATACATTTTTACAATCACTTTGATACATTGTATGATCAAATCAGATGCAAAGCTCTTGTACAGTACACTGAGCCCTTTGTGTCAATTGGTTTGCAAAGAGTGGCAGTTGTTTTTGGCTCAAGCATTGAAGCTATTGAGAAAGACCTTGTAACTTTAATAAACACTAACCAAATTCAGGTCTTCTTACCTTTTGAAACTCTTTACCTCAACCCTTCAAATTTACTGTGTCATTCTTTTACTAATAATCTTAAACAATCCTTTTTGTGTAGGCCGATATTGACCCAGTCGACAAAGTTCTACGTGTACGTAAAGTTTAAAGAAACAACTTTTTGTCGAGTTTTACAAAGTTAGAGTGTGTAATTGAACATGATCAGGTTCGTGTTGTTGTTGAAGGCACACTTTATTCGTCATTATGCTTGCATTGATACTAGTATGATCATGTTGATATTTTTGTGATGTTCAAATAGCGTGGCTAACACTTTTATA

mRNA sequence

ATGAATTTCAGGAGAAGCGAATTCGTTGACGACGCCGGAGAATCGGAAGATAATTATCGTAGTACCTCCGGCAGCGACGAAGAAGAAAAACTTAACTCTCCGACGCCGTTCTTGAAGGACAAGCTTATCGACATCCATGCCTACGCGAGCAACTACCGCCGTCGAATGAAAATCCTCCGCCTCGTATTCATCGCCGACCGCTTCGGCGACAGTAATGAACGGCGGCGGATGACTCAACTAGGAGCACTCCAAATCGCCTACGACGAGGCCAAGAAGGGCAAGGACGTCGCTCTCTTCACCAAAATCGCCGAGAAAATTAGGGGAAGATTGGGCCCTAATTACGCCATGGATGAAAATTGGCGCAATTCGGTGAATCAAGAGAAGCAAGTAATCAGCCAAATGCTACAGAGATGCACGAAGAATTCCGAGATTTGTTACGCGAACAGAATCATTTCTGCGGACGACGCAAGGATTTTATTCAATGCGGTTGCGGATTTCCACCACATCCATGGCGCACCTGCAACAGCTTTTGAATTTTACAAGAAGAGCGTACGTTTCTGCAGGACCGATGAGGATCTCTCTGAATTTTTATCGCGAGTGCTTTTGACGGTGATCGATCTTGGCCAGTTCGAACACGTTGCGAAGATGGCAGAAAAAGGAATTCAAAATCCGAAGCTTAATCCGATGACTACTGACAGTCTGCGCTGCGCTCTTGGATTGGCTCGCTTCAAGGCACAACGCTACAAAGATGCTGCTCATCAGTTCTTGGGAATCAGTAAAGAATTGGGAAACCGATTTTCGGATGTGATGGCTGCTCATGATATTGCAGCCTTTGGTGCGCTATGTGCACTTGCAACTTTTGAACCACTGGAGTTGAAGACTTTAGTTATGGATAATGAGAATTTTCAGTTCTATTTGGATTCGGCTGTTGAAGTCAAGGAGCTCATCAAGGATTTTTGTACCAGGCGTTATTCTTCATTCCTCAAACACTTTGATAACATCAAAGGATGGCTGTTGCTTGACATACATTTTTACAATCACTTTGATACATTGTATGATCAAATCAGATGCAAAGCTCTTGTACAGTACACTGAGCCCTTTGTGTCAATTGGTTTGCAAAGAGTGGCAGTTGTTTTTGGCTCAAGCATTGAAGCTATTGAGAAAGACCTTGTAACTTTAATAAACACTAACCAAATTCAGGCCGATATTGACCCAGTCGACAAAGTTCTACGTGTACGTAAAGTTTAAAGAAACAACTTTTTGTCGAGTTTTACAAAGTTAGAGTGTGTAATTGAACATGATCAGGTTCGTGTTGTTGTTGAAGGCACACTTTATTCGTCATTATGCTTGCATTGATACTAGTATGATCATGTTGATATTTTTGTGATGTTCAAATAGCGTGGCTAACACTTTTATA

Coding sequence (CDS)

ATGAATTTCAGGAGAAGCGAATTCGTTGACGACGCCGGAGAATCGGAAGATAATTATCGTAGTACCTCCGGCAGCGACGAAGAAGAAAAACTTAACTCTCCGACGCCGTTCTTGAAGGACAAGCTTATCGACATCCATGCCTACGCGAGCAACTACCGCCGTCGAATGAAAATCCTCCGCCTCGTATTCATCGCCGACCGCTTCGGCGACAGTAATGAACGGCGGCGGATGACTCAACTAGGAGCACTCCAAATCGCCTACGACGAGGCCAAGAAGGGCAAGGACGTCGCTCTCTTCACCAAAATCGCCGAGAAAATTAGGGGAAGATTGGGCCCTAATTACGCCATGGATGAAAATTGGCGCAATTCGGTGAATCAAGAGAAGCAAGTAATCAGCCAAATGCTACAGAGATGCACGAAGAATTCCGAGATTTGTTACGCGAACAGAATCATTTCTGCGGACGACGCAAGGATTTTATTCAATGCGGTTGCGGATTTCCACCACATCCATGGCGCACCTGCAACAGCTTTTGAATTTTACAAGAAGAGCGTACGTTTCTGCAGGACCGATGAGGATCTCTCTGAATTTTTATCGCGAGTGCTTTTGACGGTGATCGATCTTGGCCAGTTCGAACACGTTGCGAAGATGGCAGAAAAAGGAATTCAAAATCCGAAGCTTAATCCGATGACTACTGACAGTCTGCGCTGCGCTCTTGGATTGGCTCGCTTCAAGGCACAACGCTACAAAGATGCTGCTCATCAGTTCTTGGGAATCAGTAAAGAATTGGGAAACCGATTTTCGGATGTGATGGCTGCTCATGATATTGCAGCCTTTGGTGCGCTATGTGCACTTGCAACTTTTGAACCACTGGAGTTGAAGACTTTAGTTATGGATAATGAGAATTTTCAGTTCTATTTGGATTCGGCTGTTGAAGTCAAGGAGCTCATCAAGGATTTTTGTACCAGGCGTTATTCTTCATTCCTCAAACACTTTGATAACATCAAAGGATGGCTGTTGCTTGACATACATTTTTACAATCACTTTGATACATTGTATGATCAAATCAGATGCAAAGCTCTTGTACAGTACACTGAGCCCTTTGTGTCAATTGGTTTGCAAAGAGTGGCAGTTGTTTTTGGCTCAAGCATTGAAGCTATTGAGAAAGACCTTGTAACTTTAATAAACACTAACCAAATTCAGGCCGATATTGACCCAGTCGACAAAGTTCTACGTGTACGTAAAGTTTAA

Protein sequence

MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDENWRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALGLARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRKV
Homology
BLAST of Lcy01g006150 vs. ExPASy Swiss-Prot
Match: P45432 (COP9 signalosome complex subunit 1 OS=Arabidopsis thaliana OX=3702 GN=CSN1 PE=1 SV=2)

HSP 1 Score: 290.8 bits (743), Expect = 2.4e-77
Identity = 161/386 (41.71%), Postives = 237/386 (61.40%), Query Frame = 0

Query: 29  EKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLGALQIAYD 88
           E+ ++  P +  + +DI AYA+ Y+ R KI+RL+FIA+  G ++      Q  AL++AYD
Sbjct: 20  EETSNRRPIISGEPLDIEAYAALYKGRTKIMRLLFIANHCGGNH----ALQFDALRMAYD 79

Query: 89  EAKKGKDVALFTKIAEKIRGRLGPNYAMDENWRNSVNQEKQVISQMLQRCTKNSEICYAN 148
           E KKG++  LF ++  KI  RLG  Y MD  W  +V++  +     L+    N    Y  
Sbjct: 80  EIKKGENTQLFREVVNKIGNRLGEKYGMDLAWCEAVDRRAEQKKVKLE----NELSSYRT 139

Query: 149 RIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSEFLSRVLLTVIDLG 208
            +I  +  R+ +N   DF++  G    AF+ Y ++  +C T + +       +L  I++G
Sbjct: 140 NLIK-ESIRMGYNDFGDFYYACGMLGDAFKNYIRTRDYCTTTKHIIHMCMNAILVSIEMG 199

Query: 209 QFEHVAKMAEKGIQNPK-LNPMTTDSLRCALGLARFKAQRYKDAAHQFLGISKELGNRFS 268
           QF HV     K  QNP+ L PM    LRCA GLA  + ++YK AA +FL ++ ELGN ++
Sbjct: 200 QFTHVTSYVNKAEQNPETLEPMVNAKLRCASGLAHLELKKYKLAARKFLDVNPELGNSYN 259

Query: 269 DVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKELIKDFCTRRYSSF 328
           +V+A  DIA +G LCALA+F+  ELK  V+DN NF+ +L+   +V+ELI DF + RY+S 
Sbjct: 260 EVIAPQDIATYGGLCALASFDRSELKQKVIDNINFRNFLELVPDVRELINDFYSSRYASC 319

Query: 329 LKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQRVAVVFGSSIEAIE 388
           L++  ++K  LLLDIH ++H DTLYDQIR KAL+QYT PFVS+ L R+A  F +S+  +E
Sbjct: 320 LEYLASLKSNLLLDIHLHDHVDTLYDQIRKKALIQYTLPFVSVDLSRMADAFKTSVSGLE 379

Query: 389 KDLVTLINTNQIQADIDPVDKVLRVR 414
           K+L  LI  NQIQA ID  +K+L  R
Sbjct: 380 KELEALITDNQIQARIDSHNKILYAR 396

BLAST of Lcy01g006150 vs. ExPASy Swiss-Prot
Match: Q54QX3 (COP9 signalosome complex subunit 1 OS=Dictyostelium discoideum OX=44689 GN=csn1 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.3e-49
Identity = 129/397 (32.49%), Postives = 202/397 (50.88%), Query Frame = 0

Query: 20  RSTSGSDEEEKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQ 79
           ++T GS      N+    + ++  D+  Y +NY    KI RL+FIAD         +  +
Sbjct: 8   KNTEGSSSSSSNNNNNNSISNE-FDLETYINNYSGFTKIHRLIFIADNC-------KQLE 67

Query: 80  LGALQIAYDEAKKGKDVALFTKIAEKIRG--RLGPNYAMDENWRNSVNQEKQVISQMLQR 139
           + A  +A  E +K  ++ L+  I  K  G   L P Y  + + +NS+  +K  + Q L  
Sbjct: 68  VEAYNMALKEVQKTPNLELYNTIINKSHGILELDPVYIENLSKKNSLQLDK--LEQDLNT 127

Query: 140 CTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSEFL 199
              N         +  D  R   N + +F++  G    A + + ++  +C T + +    
Sbjct: 128 AKSN---------MVKDSIRFAQNDLGEFYYKIGDHQNALKCFIRTRDYCTTSKHVLAMC 187

Query: 200 SRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALGLARFKAQRYKDAAHQFLG 259
             ++   +D   + H      K  Q+P L+ ++   LR   GL      RYK AA +F+ 
Sbjct: 188 FNIIKLGVDTQNYTHAPSYIAKAEQSPDLDNVSNAKLRSVNGLYNLDGSRYKLAARKFIE 247

Query: 260 ISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKELIK 319
              E  N FSD+++  DIA +G LCALATF+  ELK  V+D+  F+ YL+   E++ELI 
Sbjct: 248 TPFEQSNVFSDIISPQDIAYYGGLCALATFDRNELKKKVIDDPMFKNYLELVPELRELIN 307

Query: 320 DFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQRVAV 379
           DF   +Y S LK+ D +K  LLLDIH Y H + LY +IR KALVQY  P+ S+ +  +A 
Sbjct: 308 DFYNTKYGSCLKYLDKMKPILLLDIHLYKHVEILYQRIRSKALVQYFSPYQSVDMNIMAS 367

Query: 380 VFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRK 415
            F +++  +EK++  LI  N+IQA ID  +K L  RK
Sbjct: 368 AFNTTVTLLEKEISKLIMENEIQARIDSHNKRLYARK 385

BLAST of Lcy01g006150 vs. ExPASy Swiss-Prot
Match: Q6NRT5 (COP9 signalosome complex subunit 1 OS=Xenopus laevis OX=8355 GN=csn1 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 7.9e-36
Identity = 115/415 (27.71%), Postives = 201/415 (48.43%), Query Frame = 0

Query: 26  DEEEKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLGALQI 85
           D+++ +      +++  +D+  YAS+Y   M+I RL FIADR           ++ AL++
Sbjct: 25  DDQQNMPDVNYVVENPTLDLEQYASSYSGLMRIERLQFIADRC-------PQLRVEALKM 84

Query: 86  AYDEAKKGKDVALFTKIAEKI---------------RGRLGPNYAMDENWRNSVNQEKQV 145
           A    ++  +V ++  I  K+                G + P  A+D +W  +  ++  +
Sbjct: 85  ALSFVQRTFNVDVYEDIHRKLAEASREVQNAPDAVPEGSMEPP-ALDTSWVEATRKKALL 144

Query: 146 ISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTD 205
             + L    KN    Y    I  +  R   + + D +   G  + A + Y ++  +C + 
Sbjct: 145 KLEKLDTDLKN----YKGNSIK-ESIRRGHDDLGDHYLDCGDLSNALKCYSRARDYCTSA 204

Query: 206 EDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKL----------NPMTTDSLRCALGL 265
           + +      V+   + L  + HV     K    P++                 L+CA GL
Sbjct: 205 KHVINMCLNVIKVSVYLQNWSHVLSYVSKAESTPEIAEQRGERDSQTQAVLTKLKCAAGL 264

Query: 266 ARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNE 325
           A   A++YK AA  FL  S +  + F ++++  ++A +G LCALATF+  EL+  V+ + 
Sbjct: 265 AELAARKYKQAAKCFLLASFDHCD-FPELLSPSNVAVYGGLCALATFDRQELQRNVISSS 324

Query: 326 NFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKAL 385
           +F+ +L+   +V+++I  F   +Y+S LK  D IK  LLLD++   H  TLY QIR +AL
Sbjct: 325 SFKLFLELEPQVRDIIFKFYESKYASCLKMLDEIKDNLLLDMYLAPHVRTLYTQIRNRAL 384

Query: 386 VQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRKV 416
           +QY  P+VS  + ++A  F +++ A+E +L  LI    I A ID   K+L  R V
Sbjct: 385 IQYFSPYVSADMYKMATAFNTTVSALEDELTQLILEGLINARIDSHSKILYARDV 425

BLAST of Lcy01g006150 vs. ExPASy Swiss-Prot
Match: Q99LD4 (COP9 signalosome complex subunit 1 OS=Mus musculus OX=10090 GN=Gps1 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.6e-33
Identity = 113/415 (27.23%), Postives = 202/415 (48.67%), Query Frame = 0

Query: 27  EEEKLNSP--TPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLGALQ 86
           +E+  N+P     +++  +D+  YA++Y   M+I RL FIADR           ++ AL+
Sbjct: 8   QEDPQNAPDVNYVVENPTLDLEQYAASYSGLMRIERLQFIADRCPP-------LRVEALK 67

Query: 87  IAYDEAKKGKDVALFTKIAEKIRG-----RLGPNY---------AMDENWRNSVNQEKQV 146
           +A    ++  +V ++ +I  K+       +  P+           +D  W  +  ++  +
Sbjct: 68  MALSFVQRTFNVDMYEEIHRKLSEATRELQNAPDAIPESGVEPPPLDTAWVEATRKKALL 127

Query: 147 ISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTD 206
             + L    KN    Y    I  +  R   + + D +   G  + A + Y ++  +C + 
Sbjct: 128 KLEKLDTDLKN----YKGNSIK-ESIRRGHDDLGDHYLDCGDLSNALKCYSRARDYCTSA 187

Query: 207 EDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTD----------SLRCALGL 266
           + +      V+   + L  + HV     K    P++     +           L+CA GL
Sbjct: 188 KHVINMCLNVIKVSVYLQNWSHVLSYVSKAESTPEIAEQRGERDSQTQAILTKLKCAAGL 247

Query: 267 ARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNE 326
           A   A++YK AA  FL  S +  + F ++++  ++A +G LCALATF+  EL+  V+ + 
Sbjct: 248 AELAARKYKQAAKCFLLASFDHCD-FPELLSPSNVAVYGGLCALATFDRQELQRNVISSS 307

Query: 327 NFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKAL 386
           +F+ +L+   +V+++I  F   +Y+S LK  D +K  LLLD++   H  TLY QIR +AL
Sbjct: 308 SFKLFLELEPQVRDIIFKFYESKYASCLKMLDEMKDNLLLDMYLAPHVRTLYTQIRNRAL 367

Query: 387 VQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRKV 416
           +QY  P+VS  + ++A  F +++ A+E +L  LI    I A ID   K+L  R V
Sbjct: 368 IQYFSPYVSADMHKMAAAFNTTVAALEDELTQLILEGLINARIDSHSKILYARDV 409

BLAST of Lcy01g006150 vs. ExPASy Swiss-Prot
Match: P97834 (COP9 signalosome complex subunit 1 OS=Rattus norvegicus OX=10116 GN=Gps1 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.7e-33
Identity = 112/415 (26.99%), Postives = 202/415 (48.67%), Query Frame = 0

Query: 27  EEEKLNSP--TPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLGALQ 86
           +E+  N+P     +++  +D+  YA++Y   M+I RL FIADR           ++ AL+
Sbjct: 8   QEDPQNAPDVNYVVENPTLDLEQYAASYSGLMRIERLQFIADRCPP-------LRVEALK 67

Query: 87  IAYDEAKKGKDVALFTKIAEKIRG-----RLGPNY---------AMDENWRNSVNQEKQV 146
           +A    ++  +V ++ +I  K+       +  P+           +D  W  +  ++  +
Sbjct: 68  MALSFVQRTFNVDMYEEIHRKLSEATRELQNAPDAIPESGVEPPPLDTAWVEATRKKALL 127

Query: 147 ISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTD 206
             + L    KN    Y    I  +  R   + + D +   G  + A + Y ++  +C + 
Sbjct: 128 KLEKLDTDLKN----YKGNSIK-ESIRRGHDDLGDHYLDCGDLSNALKCYSRARDYCTSA 187

Query: 207 EDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTD----------SLRCALGL 266
           + +      V+   + L  + HV     K    P++     +           L+CA GL
Sbjct: 188 KHVINMCLNVIKVSVYLQNWSHVLSYVSKAESTPEIAEQRGERDSQTQAILTKLKCAAGL 247

Query: 267 ARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNE 326
           A   A++YK AA  FL  S +  + F ++++  ++A +G LCALATF+  EL+  V+ + 
Sbjct: 248 AELAARKYKQAAKCFLLASFDHCD-FPELLSPSNVAVYGGLCALATFDRQELQRNVISSS 307

Query: 327 NFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKAL 386
           +F+ +L+   +V+++I  F   +Y+S LK  D +K  LLLD++   H  TLY QIR +AL
Sbjct: 308 SFKLFLELEPQVRDIIFKFYESKYASCLKMLDEMKDNLLLDMYLAPHVRTLYTQIRNRAL 367

Query: 387 VQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRKV 416
           +QY  P+VS  + ++A  F +++ A+E +L  +I    I A ID   K+L  R V
Sbjct: 368 IQYFSPYVSADMHKMAAAFNTTVAALEDELTQVILEGLINARIDSHSKILYARDV 409

BLAST of Lcy01g006150 vs. ExPASy TrEMBL
Match: A0A6J1H3C9 (COP9 signalosome complex subunit 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460031 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 5.3e-144
Identity = 264/411 (64.23%), Postives = 327/411 (79.56%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + +DNY     SDEE K +SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFWRSKSDGHSNKPKDNY-GNFDSDEEGKGHSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG++NERRR  QL AL+IAYD AKKG DV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGNANERRRRMQLEALRIAYDIAKKGNDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE   ++AEKG+Q P LNP+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSELLRRLIVVVIELGQFEASGEIAEKGMQYPNLNPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDN 300
           LARFKA  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGALCALATF+P+E KT V+DN
Sbjct: 241 LARFKAHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGALCALATFDPIEFKTSVIDN 300

Query: 301 ENFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKA 360
           +NF FYLDSA+EVKEL++DF  RRY S  +H DN++ WLL DI+F+NH++TLY++IR +A
Sbjct: 301 QNFHFYLDSALEVKELVRDFYLRRYCSCFRHLDNLREWLLADIYFFNHYETLYNKIRNRA 360

Query: 361 LVQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVL 411
           L+ YTEPFV++GLQRVAVVFG++IEAIEK L+ LINTNQIQAD+D ++KV+
Sbjct: 361 LIVYTEPFVAVGLQRVAVVFGTNIEAIEKQLLALINTNQIQADVDSINKVV 410

BLAST of Lcy01g006150 vs. ExPASy TrEMBL
Match: A0A6J1KWI7 (COP9 signalosome complex subunit 1-like OS=Cucurbita maxima OX=3661 GN=LOC111498865 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 1.4e-96
Identity = 190/297 (63.97%), Postives = 228/297 (76.77%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + EDNY     SDEEEK  SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFCRSKSDGHSDKPEDNY-GNFDSDEEEKGYSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG++NERRR  QL AL+IAYD AKKGKDV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGNANERRRSMQLEALRIAYDIAKKGKDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE  A++AEKG+Q P LNP+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSELLRRLIVVVIELGQFEASAEIAEKGMQYPNLNPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLV 297
           LARFK+  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGALCALATF+P+E K  +
Sbjct: 241 LARFKSHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGALCALATFDPIEFKNFI 296

BLAST of Lcy01g006150 vs. ExPASy TrEMBL
Match: A0A067HDM6 (PCI domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g013559mg PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-81
Identity = 171/393 (43.51%), Postives = 248/393 (63.10%), Query Frame = 0

Query: 22  TSGSDEEEKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLG 81
           ++G +E  +     P +  + +D+ AYAS Y+ R KI RL+FIAD    ++      QL 
Sbjct: 17  SNGGEESARTR---PIISGEQLDVEAYASLYQGRTKITRLMFIADHCDIAS-----MQLE 76

Query: 82  ALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDENWRNSVNQEKQVISQMLQRCTKN 141
           AL++AYDE KKG++  LF ++ +KI GRLGPNYAMDE W +SV++  +   + L+    N
Sbjct: 77  ALRMAYDEIKKGENTQLFREVVKKIDGRLGPNYAMDEAWCDSVDRRAEQRKEKLE----N 136

Query: 142 SEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSEFLSRVL 201
               Y   +I  +  R+ +N   DF++ HGA   AF+ Y ++  +C T + +       +
Sbjct: 137 ELNAYRTNLIK-ESIRMGYNDFGDFYYAHGALGDAFKSYVRTRDYCTTSKHIIHMCMSAI 196

Query: 202 LTVIDLGQFEHVAKMAEKGIQNPK-LNPMTTDSLRCALGLARFKAQRYKDAAHQFLGISK 261
           L  I++GQF HV     K  Q P+ L P+T   LRCA GLA  +A++YK AA +FL +  
Sbjct: 197 LVSIEMGQFTHVTSYVSKAEQTPEALEPVTIAKLRCAAGLAHLEARKYKLAARKFLEVGP 256

Query: 262 ELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKELIKDFC 321
           +LGN +++V+AA D+A +G LCALA+F+  ELK+ V+DN NF+ +L+   EV+ELI DF 
Sbjct: 257 DLGNSYNEVIAAQDVATYGGLCALASFDRAELKSKVIDNVNFRNFLELVPEVRELINDFY 316

Query: 322 TRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQRVAVVFG 381
           + RY+S L +  N+K  LLLDIH ++H +TLYDQIR KAL+QYT PFVS+ L  +A  F 
Sbjct: 317 SSRYASCLDYLGNLKANLLLDIHLHDHVETLYDQIRNKALIQYTHPFVSVDLHMMANAFK 376

Query: 382 SSIEAIEKDLVTLINTNQIQADIDPVDKVLRVR 414
           +S+  IEK+L  LI  NQIQA ID  +K+L  R
Sbjct: 377 TSVAGIEKELEALITDNQIQARIDSHNKILYAR 396

BLAST of Lcy01g006150 vs. ExPASy TrEMBL
Match: V4U862 (PCI domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10020177mg PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-81
Identity = 171/393 (43.51%), Postives = 248/393 (63.10%), Query Frame = 0

Query: 22  TSGSDEEEKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLG 81
           ++G +E  +     P +  + +D+ AYAS Y+ R KI RL+FIAD    ++      QL 
Sbjct: 17  SNGGEESARTR---PIISGEQLDVEAYASLYQGRTKITRLMFIADHCDIAS-----MQLE 76

Query: 82  ALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDENWRNSVNQEKQVISQMLQRCTKN 141
           AL++AYDE KKG++  LF ++ +KI GRLGPNYAMDE W +SV++  +   + L+    N
Sbjct: 77  ALRMAYDEIKKGENTQLFREVVKKIDGRLGPNYAMDEAWCDSVDRRAEQRKEKLE----N 136

Query: 142 SEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSEFLSRVL 201
               Y   +I  +  R+ +N   DF++ HGA   AF+ Y ++  +C T + +       +
Sbjct: 137 ELNAYRTNLIK-ESIRMGYNDFGDFYYAHGALGDAFKSYVRTRDYCTTSKHIIHMCMSAI 196

Query: 202 LTVIDLGQFEHVAKMAEKGIQNPK-LNPMTTDSLRCALGLARFKAQRYKDAAHQFLGISK 261
           L  I++GQF HV     K  Q P+ L P+T   LRCA GLA  +A++YK AA +FL +  
Sbjct: 197 LVSIEMGQFTHVTSYVSKAEQTPEALEPVTIAKLRCAAGLAHLEARKYKLAARKFLEVGP 256

Query: 262 ELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKELIKDFC 321
           +LGN +++V+AA D+A +G LCALA+F+  ELK+ V+DN NF+ +L+   EV+ELI DF 
Sbjct: 257 DLGNSYNEVIAAQDVATYGGLCALASFDRAELKSKVIDNVNFRNFLELVPEVRELINDFY 316

Query: 322 TRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQRVAVVFG 381
           + RY+S L +  N+K  LLLDIH ++H +TLYDQIR KAL+QYT PFVS+ L  +A  F 
Sbjct: 317 SSRYASCLDYLGNLKANLLLDIHLHDHVETLYDQIRNKALIQYTHPFVSVDLHMMANAFK 376

Query: 382 SSIEAIEKDLVTLINTNQIQADIDPVDKVLRVR 414
           +S+  IEK+L  LI  NQIQA ID  +K+L  R
Sbjct: 377 TSVAGIEKELEALITDNQIQARIDSHNKILYAR 396

BLAST of Lcy01g006150 vs. ExPASy TrEMBL
Match: A0A2P5CNT9 (26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit OS=Trema orientale OX=63057 GN=TorRG33x02_278450 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 2.7e-79
Identity = 171/399 (42.86%), Postives = 246/399 (61.65%), Query Frame = 0

Query: 16  EDNYRSTSGSDEEEKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERR 75
           ++ Y +     +EEK N   P +  + +DI AYAS Y  R KI RL+FIAD      E  
Sbjct: 14  DEIYANGGVGGDEEKRN--RPIISGEQLDIEAYASLYAGRTKITRLLFIADHC----ENN 73

Query: 76  RMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDENWRNSVNQEKQVISQML 135
           +  QL AL++AYDE KKG++  LF ++ +KI GRLG NY MD  WR+SV++  +   + L
Sbjct: 74  QSMQLEALRMAYDEIKKGENTQLFREVVQKINGRLGHNYVMDSAWRDSVDRRAEQKKEKL 133

Query: 136 QRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSE 195
           +    N    Y   +I  +  R+ +N   DF++ HGA   AF+ Y ++  +C T + +  
Sbjct: 134 E----NELNAYRTNLIK-ESIRMGYNDFGDFYYAHGALGDAFKHYVRTRDYCTTSKHIIH 193

Query: 196 FLSRVLLTVIDLGQFEHVAKMAEKGIQNPK-LNPMTTDSLRCALGLARFKAQRYKDAAHQ 255
                +L  I++GQF HV     K  Q+ + L+P+ T  LRCA GLA  +A++YK AA +
Sbjct: 194 MCMNAILVSIEMGQFTHVTSYVSKAEQSSETLDPILTGKLRCAAGLAHLEAKKYKLAARK 253

Query: 256 FLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKE 315
           FL  S ELG+ +++V+A  D+A +G LCALA+F+  ELK  V+DN NF+ +L+   E++E
Sbjct: 254 FLETSAELGSHYNEVIAPQDVATYGGLCALASFDRTELKNKVIDNLNFRNFLELVPEIRE 313

Query: 316 LIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQR 375
           LI DF +  Y+S L +  N+K  LLLDIH ++H +TLYDQIR KAL+QYT PFVS+ L  
Sbjct: 314 LINDFYSSHYASCLDYLGNLKANLLLDIHLHDHVETLYDQIRNKALIQYTHPFVSVDLHM 373

Query: 376 VAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVR 414
           +A  F +SI  +EK+L  LI  NQIQA ID  +K+L  R
Sbjct: 374 MANAFKTSITGLEKELEALITENQIQARIDSHNKILYAR 401

BLAST of Lcy01g006150 vs. NCBI nr
Match: XP_022958876.1 (COP9 signalosome complex subunit 1-like [Cucurbita moschata])

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-143
Identity = 264/411 (64.23%), Postives = 327/411 (79.56%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + +DNY     SDEE K +SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFWRSKSDGHSNKPKDNY-GNFDSDEEGKGHSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG++NERRR  QL AL+IAYD AKKG DV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGNANERRRRMQLEALRIAYDIAKKGNDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE   ++AEKG+Q P LNP+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSELLRRLIVVVIELGQFEASGEIAEKGMQYPNLNPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDN 300
           LARFKA  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGALCALATF+P+E KT V+DN
Sbjct: 241 LARFKAHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGALCALATFDPIEFKTSVIDN 300

Query: 301 ENFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKA 360
           +NF FYLDSA+EVKEL++DF  RRY S  +H DN++ WLL DI+F+NH++TLY++IR +A
Sbjct: 301 QNFHFYLDSALEVKELVRDFYLRRYCSCFRHLDNLREWLLADIYFFNHYETLYNKIRNRA 360

Query: 361 LVQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVL 411
           L+ YTEPFV++GLQRVAVVFG++IEAIEK L+ LINTNQIQAD+D ++KV+
Sbjct: 361 LIVYTEPFVAVGLQRVAVVFGTNIEAIEKQLLALINTNQIQADVDSINKVV 410

BLAST of Lcy01g006150 vs. NCBI nr
Match: KAG6574781.1 (COP9 signalosome complex subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 518.1 bits (1333), Expect = 7.1e-143
Identity = 264/411 (64.23%), Postives = 326/411 (79.32%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + +DNY     SDEE K +SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFWRSKSDGHSIKPKDNY-GNFDSDEEGKGHSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG +NERRR  QL AL+IAYD AKKG DV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGHANERRRRMQLEALRIAYDIAKKGNDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE   ++AEKG+Q P LNP+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSEVLRRLIVVVIELGQFEASGEIAEKGMQYPNLNPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMDN 300
           LARFKA  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGALCALATF+P+E KT V+DN
Sbjct: 241 LARFKAHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGALCALATFDPIEFKTSVIDN 300

Query: 301 ENFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKA 360
           +NF FYLDSA+EVKEL++DF  RRY S  +H DN++ WLL DI+F+NH++TLY++IR +A
Sbjct: 301 QNFHFYLDSALEVKELVRDFYLRRYCSCFRHLDNLREWLLADIYFFNHYETLYNKIRNRA 360

Query: 361 LVQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVL 411
           L+ YTEPFV++GLQRVAVVFG++IEAIEK L+ LINTNQIQAD+D ++KV+
Sbjct: 361 LIVYTEPFVAVGLQRVAVVFGTNIEAIEKQLLALINTNQIQADVDSINKVV 410

BLAST of Lcy01g006150 vs. NCBI nr
Match: XP_023005986.1 (COP9 signalosome complex subunit 1-like [Cucurbita maxima])

HSP 1 Score: 363.2 bits (931), Expect = 2.9e-96
Identity = 190/297 (63.97%), Postives = 228/297 (76.77%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + EDNY     SDEEEK  SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFCRSKSDGHSDKPEDNY-GNFDSDEEEKGYSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG++NERRR  QL AL+IAYD AKKGKDV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGNANERRRSMQLEALRIAYDIAKKGKDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE  A++AEKG+Q P LNP+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSELLRRLIVVVIELGQFEASAEIAEKGMQYPNLNPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLV 297
           LARFK+  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGALCALATF+P+E K  +
Sbjct: 241 LARFKSHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGALCALATFDPIEFKNFI 296

BLAST of Lcy01g006150 vs. NCBI nr
Match: XP_023547756.1 (COP9 signalosome complex subunit 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.5 bits (929), Expect = 5.0e-96
Identity = 190/297 (63.97%), Postives = 228/297 (76.77%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + EDNY     SDEEEK  SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFWRSKSDGHSDKPEDNY-GNFDSDEEEKGYSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG++NERRR  QL AL+IAYD AKKGKDV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGNANERRRRMQLEALRIAYDIAKKGKDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE  A++AEKG+Q P L+P+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSELLRRLIVVVIELGQFEASAEIAEKGMQYPNLHPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLV 297
           LARFKA  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGALCALATF+P+E K  +
Sbjct: 241 LARFKAHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGALCALATFDPIEFKNFI 296

BLAST of Lcy01g006150 vs. NCBI nr
Match: KAG7013361.1 (COP9 signalosome complex subunit 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.0 bits (863), Expect = 2.2e-88
Identity = 178/282 (63.12%), Postives = 214/282 (75.89%), Query Frame = 0

Query: 1   MNFRRSEFVDDAGESEDNYRSTSGSDEEEKLNSPTPFLK-DKLIDIHAYASNYRRRMKIL 60
           M+F RS+    + + +DNY     SDEE K +SPTPFLK  K  DI AYASNY R  KIL
Sbjct: 1   MHFWRSKSDGHSNKPKDNY-GNFDSDEEGKGHSPTPFLKGGKHFDIQAYASNYHRSTKIL 60

Query: 61  RLVFIADRFGDSNERRRMTQLGALQIAYDEAKKGKDVALFTKIAEKIRGRLGPNYAMDEN 120
           RL+FIADRFG++NERRR  QL AL+IAYD AKKG DV +F KI  KI GRLGPNY MDEN
Sbjct: 61  RLIFIADRFGNANERRRRMQLEALRIAYDIAKKGNDVNVFIKIVRKIDGRLGPNYDMDEN 120

Query: 121 WRNSVNQEKQVISQMLQRCTKNSEICYANRIISADDARILFNAVADFHHIHGAPATAFEF 180
           W NSV + ++V+S+MLQRC KN E  Y  R I A+DAR +FN++A F+  HGA   A+ F
Sbjct: 121 WCNSVIEGRRVMSEMLQRCAKNCEKSYTERTICAEDARNVFNSIAVFYEDHGALTRAYAF 180

Query: 181 YKKSVRFCRTDEDLSEFLSRVLLTVIDLGQFEHVAKMAEKGIQNPKLNPMTTDSLRCALG 240
           YKKSV FCR+D++ SE L R+++ VI+LGQFE   ++AEKG+Q P LNP+T ++LRCALG
Sbjct: 181 YKKSVHFCRSDKNYSELLRRLIVVVIELGQFEASGEIAEKGMQYPNLNPITMETLRCALG 240

Query: 241 LARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGAL 282
           LARFKA  YKDAAHQ LG+SKELGNRFS VMAAHDIA FGAL
Sbjct: 241 LARFKAHCYKDAAHQLLGVSKELGNRFSKVMAAHDIATFGAL 281

BLAST of Lcy01g006150 vs. TAIR 10
Match: AT3G61140.1 (26S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain )

HSP 1 Score: 290.8 bits (743), Expect = 1.7e-78
Identity = 161/386 (41.71%), Postives = 237/386 (61.40%), Query Frame = 0

Query: 29  EKLNSPTPFLKDKLIDIHAYASNYRRRMKILRLVFIADRFGDSNERRRMTQLGALQIAYD 88
           E+ ++  P +  + +DI AYA+ Y+ R KI+RL+FIA+  G ++      Q  AL++AYD
Sbjct: 20  EETSNRRPIISGEPLDIEAYAALYKGRTKIMRLLFIANHCGGNH----ALQFDALRMAYD 79

Query: 89  EAKKGKDVALFTKIAEKIRGRLGPNYAMDENWRNSVNQEKQVISQMLQRCTKNSEICYAN 148
           E KKG++  LF ++  KI  RLG  Y MD  W  +V++  +     L+    N    Y  
Sbjct: 80  EIKKGENTQLFREVVNKIGNRLGEKYGMDLAWCEAVDRRAEQKKVKLE----NELSSYRT 139

Query: 149 RIISADDARILFNAVADFHHIHGAPATAFEFYKKSVRFCRTDEDLSEFLSRVLLTVIDLG 208
            +I  +  R+ +N   DF++  G    AF+ Y ++  +C T + +       +L  I++G
Sbjct: 140 NLIK-ESIRMGYNDFGDFYYACGMLGDAFKNYIRTRDYCTTTKHIIHMCMNAILVSIEMG 199

Query: 209 QFEHVAKMAEKGIQNPK-LNPMTTDSLRCALGLARFKAQRYKDAAHQFLGISKELGNRFS 268
           QF HV     K  QNP+ L PM    LRCA GLA  + ++YK AA +FL ++ ELGN ++
Sbjct: 200 QFTHVTSYVNKAEQNPETLEPMVNAKLRCASGLAHLELKKYKLAARKFLDVNPELGNSYN 259

Query: 269 DVMAAHDIAAFGALCALATFEPLELKTLVMDNENFQFYLDSAVEVKELIKDFCTRRYSSF 328
           +V+A  DIA +G LCALA+F+  ELK  V+DN NF+ +L+   +V+ELI DF + RY+S 
Sbjct: 260 EVIAPQDIATYGGLCALASFDRSELKQKVIDNINFRNFLELVPDVRELINDFYSSRYASC 319

Query: 329 LKHFDNIKGWLLLDIHFYNHFDTLYDQIRCKALVQYTEPFVSIGLQRVAVVFGSSIEAIE 388
           L++  ++K  LLLDIH ++H DTLYDQIR KAL+QYT PFVS+ L R+A  F +S+  +E
Sbjct: 320 LEYLASLKSNLLLDIHLHDHVDTLYDQIRKKALIQYTLPFVSVDLSRMADAFKTSVSGLE 379

Query: 389 KDLVTLINTNQIQADIDPVDKVLRVR 414
           K+L  LI  NQIQA ID  +K+L  R
Sbjct: 380 KELEALITDNQIQARIDSHNKILYAR 396

BLAST of Lcy01g006150 vs. TAIR 10
Match: AT3G12850.1 (COP9 signalosome complex-related / CSN complex-related )

HSP 1 Score: 62.4 bits (150), Expect = 1.0e-09
Identity = 42/113 (37.17%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 43  IDIHAYASNYRRRMKILRLVFIADR-----FGDSNERRRMTQLGALQIAYDEAKKGK--- 102
           +D+ AY+ NY  R  +LRL+ IA+      F D  E      L AL++AYD  +      
Sbjct: 11  LDVEAYSRNYEGRNLVLRLLHIAENSVGPMFTDDLE------LEALRLAYDLIRNDGIVC 70

Query: 103 DVALFTKIAEKIRGRLGPNYAMDENWR----NSVNQEKQVISQMLQRCTKNSE 144
           D+ LFT IAEKI+GRLG  Y +DE WR    N+  ++  ++   L+  T   E
Sbjct: 71  DMRLFTVIAEKIQGRLGEGYLLDEKWRLTKNNNFVKQDSILRNDLESATDELE 117

BLAST of Lcy01g006150 vs. TAIR 10
Match: AT4G24820.1 (26S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain )

HSP 1 Score: 51.6 bits (122), Expect = 1.8e-06
Identity = 38/176 (21.59%), Postives = 71/176 (40.34%), Query Frame = 0

Query: 239 GLARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMD 298
           GL     + +K AA  FL           ++        +  L ++ T + + LK  V+D
Sbjct: 183 GLYCMSTRNFKKAASLFLDSISTFTT--YEIFPYETFIFYTVLTSIITLDRVSLKQKVVD 242

Query: 299 NENFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCK 358
                  L     + E +      +Y +F   F  +   +  D + Y HF     ++R  
Sbjct: 243 APEILTVLGKIPFLSEFLNSLYECQYKAFFSAFAGMAVQIKYDRYLYPHFRFYMREVRTV 302

Query: 359 ALVQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRK 415
              Q+ E + S+ ++ +A  FG S++ I+++L   I   ++   ID V  VL   +
Sbjct: 303 VYSQFLESYKSVTVEAMAKAFGVSVDFIDQELSRFIAAGKLHCKIDKVAGVLETNR 356

BLAST of Lcy01g006150 vs. TAIR 10
Match: AT4G24820.2 (26S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain )

HSP 1 Score: 51.6 bits (122), Expect = 1.8e-06
Identity = 38/176 (21.59%), Postives = 71/176 (40.34%), Query Frame = 0

Query: 239 GLARFKAQRYKDAAHQFLGISKELGNRFSDVMAAHDIAAFGALCALATFEPLELKTLVMD 298
           GL     + +K AA  FL           ++        +  L ++ T + + LK  V+D
Sbjct: 183 GLYCMSTRNFKKAASLFLDSISTFTT--YEIFPYETFIFYTVLTSIITLDRVSLKQKVVD 242

Query: 299 NENFQFYLDSAVEVKELIKDFCTRRYSSFLKHFDNIKGWLLLDIHFYNHFDTLYDQIRCK 358
                  L     + E +      +Y +F   F  +   +  D + Y HF     ++R  
Sbjct: 243 APEILTVLGKIPFLSEFLNSLYECQYKAFFSAFAGMAVQIKYDRYLYPHFRFYMREVRTV 302

Query: 359 ALVQYTEPFVSIGLQRVAVVFGSSIEAIEKDLVTLINTNQIQADIDPVDKVLRVRK 415
              Q+ E + S+ ++ +A  FG S++ I+++L   I   ++   ID V  VL   +
Sbjct: 303 VYSQFLESYKSVTVEAMAKAFGVSVDFIDQELSRFIAAGKLHCKIDKVAGVLETNR 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P454322.4e-7741.71COP9 signalosome complex subunit 1 OS=Arabidopsis thaliana OX=3702 GN=CSN1 PE=1 ... [more]
Q54QX31.3e-4932.49COP9 signalosome complex subunit 1 OS=Dictyostelium discoideum OX=44689 GN=csn1 ... [more]
Q6NRT57.9e-3627.71COP9 signalosome complex subunit 1 OS=Xenopus laevis OX=8355 GN=csn1 PE=2 SV=1[more]
Q99LD41.6e-3327.23COP9 signalosome complex subunit 1 OS=Mus musculus OX=10090 GN=Gps1 PE=1 SV=1[more]
P978343.7e-3326.99COP9 signalosome complex subunit 1 OS=Rattus norvegicus OX=10116 GN=Gps1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1H3C95.3e-14464.23COP9 signalosome complex subunit 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KWI71.4e-9663.97COP9 signalosome complex subunit 1-like OS=Cucurbita maxima OX=3661 GN=LOC111498... [more]
A0A067HDM63.8e-8143.51PCI domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g013559mg PE=... [more]
V4U8623.8e-8143.51PCI domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10020177mg... [more]
A0A2P5CNT92.7e-7942.8626S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit OS=Trema... [more]
Match NameE-valueIdentityDescription
XP_022958876.11.1e-14364.23COP9 signalosome complex subunit 1-like [Cucurbita moschata][more]
KAG6574781.17.1e-14364.23COP9 signalosome complex subunit 1, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023005986.12.9e-9663.97COP9 signalosome complex subunit 1-like [Cucurbita maxima][more]
XP_023547756.15.0e-9663.97COP9 signalosome complex subunit 1-like [Cucurbita pepo subsp. pepo][more]
KAG7013361.12.2e-8863.12COP9 signalosome complex subunit 1, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
AT3G61140.11.7e-7841.7126S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain [more]
AT3G12850.11.0e-0937.17COP9 signalosome complex-related / CSN complex-related [more]
AT4G24820.11.8e-0621.5926S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain [more]
AT4G24820.21.8e-0621.5926S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00753PINTcoord: 346..415
e-value: 5.2E-5
score: 32.6
NoneNo IPR availableGENE3D1.25.40.570coord: 42..396
e-value: 1.2E-89
score: 302.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
IPR000717Proteasome component (PCI) domainSMARTSM00088PINT_4coord: 346..415
e-value: 5.2E-5
score: 32.6
IPR000717Proteasome component (PCI) domainPFAMPF01399PCIcoord: 312..413
e-value: 9.0E-13
score: 48.7
IPR000717Proteasome component (PCI) domainPROSITEPS50250PCIcoord: 232..415
score: 14.188356
IPR04513526S proteasome regulatory subunit Rpn7, N-terminalPFAMPF10602RPN7coord: 155..294
e-value: 5.3E-11
score: 42.5
IPR01958526S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1PANTHERPTHR1414526S PROTESOME SUBUNIT 6coord: 26..414
IPR033008COP9 signalosome complex subunit 1PANTHERPTHR14145:SF2COP9 SIGNALOSOME COMPLEX SUBUNIT 1coord: 26..414
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 341..413

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g006150.1Lcy01g006150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000338 protein deneddylation
cellular_component GO:0008180 COP9 signalosome
cellular_component GO:0005737 cytoplasm