Lcy01g005450 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g005450
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr01: 7557467 .. 7557970 (+)
RNA-Seq ExpressionLcy01g005450
SyntenyLcy01g005450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCCCAAGCTAAGCATCATGGAGACTATCGAGATCGAGAGCGGTCGAGAGATGCTGATGACTCAAGCCTAACTGTGTGGACTCCACCACCCCAACACCATTTCAAGTTGAATGTGGATGCAACACTAGCAAATGGAGATATGAGGTCAGACATGATTATCAGAAATTCAAAGAGAGATTCAATGGCAAGTGTAGAATATTTCAGGAGAACGAGTTGCTCAGTGGAGTGGGTAGAAGCACAGACTCTTGTTGACGACATTCAGCTAGCGATGGAGTCGGGATTATCACCAATATGGATAGAGATGGATTTCAAAATTGTATGGAATCTACTTCATGACCACGACAATTATTATAATGAGATTGCTCCCCTAATTCACCATCTCAAGCAATTGACCATTGATAATCACATCAGAGGTTTTTTACTGACAAAAAGGGAAGGCAACAAAGCGGCGCACCAGCTGGCTATTCATGCTCGCACATCAAAACCTTCAGAAGTTTGG

mRNA sequence

ATGGAGGCCCAAGCTAAGCATCATGGAGACTATCGAGATCGAGAGCGGTCGAGAGATGCTGATGACTCAAGCCTAACTGTGTGGACTCCACCACCCCAACACCATTTCAAGTTGAATGTGGATGCAACACTAGCAAATGGAGATATGAGGTCAGACATGATTATCAGAAATTCAAAGAGAGATTCAATGGCAAGTGTAGAATATTTCAGGAGAACGAGTTGCTCAGTGGAGTGGGTAGAAGCACAGACTCTTGTTGACGACATTCAGCTAGCGATGGAGTCGGGATTATCACCAATATGGATAGAGATGGATTTCAAAATTGTATGGAATCTACTTCATGACCACGACAATTATTATAATGAGATTGCTCCCCTAATTCACCATCTCAAGCAATTGACCATTGATAATCACATCAGAGGTTTTTTACTGACAAAAAGGGAAGGCAACAAAGCGGCGCACCAGCTGGCTATTCATGCTCGCACATCAAAACCTTCAGAAGTTTGG

Coding sequence (CDS)

ATGGAGGCCCAAGCTAAGCATCATGGAGACTATCGAGATCGAGAGCGGTCGAGAGATGCTGATGACTCAAGCCTAACTGTGTGGACTCCACCACCCCAACACCATTTCAAGTTGAATGTGGATGCAACACTAGCAAATGGAGATATGAGGTCAGACATGATTATCAGAAATTCAAAGAGAGATTCAATGGCAAGTGTAGAATATTTCAGGAGAACGAGTTGCTCAGTGGAGTGGGTAGAAGCACAGACTCTTGTTGACGACATTCAGCTAGCGATGGAGTCGGGATTATCACCAATATGGATAGAGATGGATTTCAAAATTGTATGGAATCTACTTCATGACCACGACAATTATTATAATGAGATTGCTCCCCTAATTCACCATCTCAAGCAATTGACCATTGATAATCACATCAGAGGTTTTTTACTGACAAAAAGGGAAGGCAACAAAGCGGCGCACCAGCTGGCTATTCATGCTCGCACATCAAAACCTTCAGAAGTTTGG

Protein sequence

MEAQAKHHGDYRDRERSRDADDSSLTVWTPPPQHHFKLNVDATLANGDMRSDMIIRNSKRDSMASVEYFRRTSCSVEWVEAQTLVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHIRGFLLTKREGNKAAHQLAIHARTSKPSEVW
Homology
BLAST of Lcy01g005450 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.7e-11
Identity = 45/144 (31.25%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 27  VWTPPPQHHFKLNVDATLANGDMRSDM--IIRNSKRDSMASVEYFRRTSCSVEWVEAQTL 86
           +W PP +  +K+N DA+    D  + +  IIRN +   MAS   +     SV+  EA   
Sbjct: 33  LWLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVA 92

Query: 87  VDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHIRGFLLT 146
           V+ +QLA + G++P+ +E D   ++NL        +E   ++   K     +    F   
Sbjct: 93  VEGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHASFNFV 152

Query: 147 KREGNKAAHQLAIHARTSKPSEVW 169
           KREGNKAAH LA  A   +   +W
Sbjct: 153 KREGNKAAHMLARRALLLREFSIW 176

BLAST of Lcy01g005450 vs. ExPASy TrEMBL
Match: A0A2N9EU90 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.1e-10
Identity = 52/154 (33.77%), Postives = 76/154 (49.35%), Query Frame = 0

Query: 17  SRDADDSSLTVWTPPPQHHFKLNVDATL--ANGDMRSDMIIRNSKRDSMASVEYFRRTSC 76
           +R A       W+PP    +K+N DA     + ++   +IIRN   + MAS+ +      
Sbjct: 498 TRQAVPLQRVTWSPPQPGRYKVNYDAAFFGDSNEVGIGVIIRNHNGEVMASLCHRIHYPH 557

Query: 77  SVEWVEAQTLVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTI 136
           SVE +EA      IQ+A++ GL  I IE D + V + L +H   +     LI+     T 
Sbjct: 558 SVEAMEAYAARSAIQMAIDLGLQAIDIEGDSQTVVDALLNHAPCFTLYGNLINDTNIATQ 617

Query: 137 DNHIRGFLLTKREGNKAAHQLAIHARTSKPSEVW 169
             H   FL  KR+GN  AH +A  A+ S+P EVW
Sbjct: 618 SCHFVQFLHVKRDGNSIAHSIAKRAKFSQPFEVW 651

BLAST of Lcy01g005450 vs. ExPASy TrEMBL
Match: A0A2N9GET2 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25820 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.9e-09
Identity = 51/146 (34.93%), Postives = 70/146 (47.95%), Query Frame = 0

Query: 28  WTPPPQHHFKLNVDATLAN--GDMRSDMIIRNSKRDSMASVEYFRRTSCSVEWVEAQTLV 87
           W PP     K+N D  + N  G+    +IIRNS+   MAS+        SVE VEA    
Sbjct: 568 WKPPAMGRVKINYDGAVFNETGEAGIGVIIRNSQGAVMASLSQRILFPHSVEAVEATAAR 627

Query: 88  DDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLK---QLTIDNHIRGFL 147
             +QL ++ G   + +E D   +   L   +  Y     +I+  K   QL +  H   FL
Sbjct: 628 LAVQLVLDLGFKEVEVEGDSVNIVQALSQTETSYTLYGHIINDTKLASQLLVSVH---FL 687

Query: 148 LTKREGNKAAHQLAIHARTSKPSEVW 169
             KREGNK AH LA  AR ++P +VW
Sbjct: 688 HVKREGNKVAHSLAKRARCNQPFQVW 710

BLAST of Lcy01g005450 vs. ExPASy TrEMBL
Match: A0A2N9FRW5 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17561 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 7.7e-09
Identity = 49/150 (32.67%), Postives = 71/150 (47.33%), Query Frame = 0

Query: 21  DDSSLTVWTPPPQHHFKLNVDATLANGDMRSDM--IIRNSKRDSMASVEYFRRTSCSVEW 80
           + S++T WTPP    +K+N D  +      + +  IIRN + ++MAS+        SVE 
Sbjct: 51  EPSTVTKWTPPSSGQYKVNFDGAIFTESNEAGLGAIIRNHRGEAMASLCQRIPYPHSVEA 110

Query: 81  VEAQTLVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHI 140
           VEA      + L ++ GL  + IE D   +   L  +   Y     LI   K L  +   
Sbjct: 111 VEASATRTAVNLILDLGLREVDIEGDSLKIITALKQNSPCYTLYGHLIMETKTLAQNLTS 170

Query: 141 RGFLLTKREGNKAAHQLAIHARTSKPSEVW 169
             F+  KR+GN  AH LA  AR  +P EVW
Sbjct: 171 FQFMHVKRDGNTVAHSLAKRARLCEPLEVW 200

BLAST of Lcy01g005450 vs. ExPASy TrEMBL
Match: A0A2N9HG19 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38757 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.3e-08
Identity = 50/143 (34.97%), Postives = 68/143 (47.55%), Query Frame = 0

Query: 28   WTPPPQHHFKLNVDATL--ANGDMRSDMIIRNSKRDSMASVEYFRRTSCSVEWVEAQTLV 87
            W PP    +K+N D  +     +    +IIRN K ++MAS+ +      SVE +EA    
Sbjct: 1453 WKPPQPGRYKINYDGAMFAETNEAGIGVIIRNHKGEAMASMCHSIHYPHSVEAMEAYAAR 1512

Query: 88   DDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHIRGFLLTK 147
               QLA++ G++ + IE D K + N L            LI+  K          FL  K
Sbjct: 1513 SAAQLAIDLGITEVDIEGDSKTIVNALLATTPCCTLYGHLINDTKNAAHSCLSVQFLHVK 1572

Query: 148  REGNKAAHQLAIHARTSKPSEVW 169
            REGN  AH LA  AR S+P EVW
Sbjct: 1573 REGNSLAHSLAKRARFSQPLEVW 1595

BLAST of Lcy01g005450 vs. NCBI nr
Match: MBA0755499.1 (hypothetical protein [Gossypium gossypioides])

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-11
Identity = 52/162 (32.10%), Postives = 77/162 (47.53%), Query Frame = 0

Query: 10  DYRDRERS-RDADDSSLTVWTPPPQHHFKLNVDA--TLANGDMRSDMIIRNSKRDSMASV 69
           +YR    S +     S+  W+PPPQ  FK+NVDA  ++AN  + +D IIRN +   M S 
Sbjct: 150 EYRSSAESLKHPQPRSMVKWSPPPQGWFKVNVDAGVSIANNRVVTDFIIRNDEGFIMGSG 209

Query: 70  EYFRRTSCSVEWVEAQTLVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLI 129
                   SV   EA  ++  +Q A++ G S + +E D ++V N +      Y+E  P  
Sbjct: 210 FQRHNLVLSVVIAEAIAVLHGLQFALDMGFSRVILESDSRLVVNNIQKSSEDYSESRPFT 269

Query: 130 HHLKQLTIDNHIRGFLLTKREGNKAAHQLAIHARTSKPSEVW 169
             +K L    H   F    REGN AAH L +    ++    W
Sbjct: 270 WDVKNLARKFHCCRFQFVAREGNGAAHALVVEGMQAEGDSFW 311

BLAST of Lcy01g005450 vs. NCBI nr
Match: KAG6608327.1 (hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 79.3 bits (194), Expect = 3.4e-11
Identity = 51/144 (35.42%), Postives = 73/144 (50.69%), Query Frame = 0

Query: 28  WTPPPQHHFKLNVDATLANGDMRSDM----IIRNSKRDSMASVEYFRRTSCSVEWVEAQT 87
           W   P    KL+VDAT+ N +M   M    I +   +DS                     
Sbjct: 22  WVSLPHDQDKLSVDATMHNEEMGCQMGLFEIPKALGKDSSRG------------------ 81

Query: 88  LVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHIRGFLL 147
                QL M +GL+ +W +++ K+VW LL D D ++ EIA LI HLK L+ ++ I+GFL 
Sbjct: 82  ----NQLGMATGLTSVWPKVNSKVVWTLLMDQDQHFIEIAHLIKHLKHLSQNHCIKGFLP 141

Query: 148 TKREGNKAAHQLAIHARTSKPSEV 168
           T ++  KA H LAIHA  +K  E+
Sbjct: 142 TPKDDTKATHTLAIHAWHTKHLEI 143

BLAST of Lcy01g005450 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 79.3 bits (194), Expect = 3.4e-11
Identity = 45/144 (31.25%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 27  VWTPPPQHHFKLNVDATLANGDMRSDM--IIRNSKRDSMASVEYFRRTSCSVEWVEAQTL 86
           +W PP +  +K+N DA+    D  + +  IIRN +   MAS   +     SV+  EA   
Sbjct: 33  LWLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVA 92

Query: 87  VDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHIRGFLLT 146
           V+ +QLA + G++P+ +E D   ++NL        +E   ++   K     +    F   
Sbjct: 93  VEGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHASFNFV 152

Query: 147 KREGNKAAHQLAIHARTSKPSEVW 169
           KREGNKAAH LA  A   +   +W
Sbjct: 153 KREGNKAAHMLARRALLLREFSIW 176

BLAST of Lcy01g005450 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-09
Identity = 50/153 (32.68%), Postives = 70/153 (45.75%), Query Frame = 0

Query: 19  DADDSSLTVWTPPPQHHFKLNVDATLANGDMRS--DMIIRNSKRDSMASVEYFRRTSCSV 78
           D     ++ W PP    FK+NVDA    G+  +  D++IR+S  + + S   F      V
Sbjct: 64  DGRGGRVSAWCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDV 123

Query: 79  EWVEAQTLVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDN 138
              E     + + LA+E+GL P  IE D   V+NLL       +EI  L   ++ +    
Sbjct: 124 TLAECLAAQEGVCLAIEAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSL 183

Query: 139 HI-RGFLLTKREGNKAAHQLAIHARTSKPSEVW 169
           HI  GF    REGN  AH LA     S+   VW
Sbjct: 184 HIGGGFSFVNREGNSGAHTLARMGMVSESFHVW 216

BLAST of Lcy01g005450 vs. NCBI nr
Match: XP_023915006.1 (uncharacterized protein LOC112026546 [Quercus suber])

HSP 1 Score: 73.2 bits (178), Expect = 2.5e-09
Identity = 51/148 (34.46%), Postives = 76/148 (51.35%), Query Frame = 0

Query: 23  SSLTVWTPPPQHHFKLNVDATLANGDMRSD--MIIRNSKRDSMASVEYFRRTSCSVEWVE 82
           SSL  W PPP    K+NVD   ++    S   +IIR+S+  S+A+      +    +  E
Sbjct: 343 SSLINWRPPPAGFTKINVDGAASDDGRPSSTGVIIRDSQGSSIAASCRVLSSPFPADISE 402

Query: 83  AQTLVDDIQLAMESGLSPIWIEMDFKIVWNLLHDHDNYYNEIAPLIHHLKQLTIDNHIRG 142
           A  L D + LA+E GLS +  E D   +   +++  N   EI  ++ ++K L        
Sbjct: 403 ALALQDGVMLALELGLSKVIFESDALSIIQAINE-GNVGGEIGHIMQNIKDLASSFSWCT 462

Query: 143 FLLTKREGNKAAHQLAIHARTSKPSEVW 169
           F   KREGN+AAH+LA  AR +  S+VW
Sbjct: 463 FQHQKREGNRAAHELARAARMTGISQVW 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CIF11.7e-1131.25uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A2N9EU903.1e-1033.77Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1[more]
A0A2N9GET25.9e-0934.93Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9FRW57.7e-0932.67RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17561 ... [more]
A0A2N9HG191.3e-0834.97Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38757 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
MBA0755499.11.2e-1132.10hypothetical protein [Gossypium gossypioides][more]
KAG6608327.13.4e-1135.42hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022140628.13.4e-1131.25uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_022135942.11.4e-0932.68uncharacterized protein LOC111007775 [Momordica charantia][more]
XP_023915006.12.5e-0934.46uncharacterized protein LOC112026546 [Quercus suber][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 36..164
e-value: 2.6E-5
score: 26.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 50..158
e-value: 1.6E-10
score: 40.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 16..162
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 74..156
e-value: 6.63147E-10
score: 51.9312
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 75..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g005450.1Lcy01g005450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity