Lcy01g003300 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g003300
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionDNA topoisomerase 2
LocationChr01: 3955962 .. 3961978 (-)
RNA-Seq ExpressionLcy01g003300
SyntenyLcy01g003300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCGAGTATGAGTCTTGGAAAGAGAACTTGAGAGGCAATGCAAGTGGCTGGTCAATCAAGTACTATAAGGTCGGTCTTCTGAACTTAAATATAGTAACTTCATTGTGTAAGTTGTATTTCTTCAAATTCTATTTGAAAAGATATTCATGTTTCAGTGCATTAATTCGTTGTCTTGATTGCGTAGGGGTTGGGAACAAGCACATCGAAAGAAGGAAGAGAGTACTTTGATCAGCTGGAAAAACATAAGAAAGAATTTATTTGGAAAGATGCCCATGATGGAGAGGCCATTGAACTTGCGTTCAGTAAGAAGAAGATTGAAGAGAGGAAAAATTGGCTCCGTCAATTTGAGGTAAGAAGTCTCATCAGTTAGCCCATGTTTTTTTCTTTCCTCTTGCAAGGAGTTCATTGTGTTACTGATGTGATGTCACTCTCTTATGTAACGCTTGCAGCCTGGTATCCATCTAGATCAGACAGAAAAATTGATAAAATACAGTGACTTCATCAACAAGGAGCTTATATTATTTTCGATAGCTGATTTACAAAGGTCTGTTCCCTCAATGGTTGATGGCCTCAAGCCTGGCCAGAGGAAGATCCTTTTCTGTTCTTTCAAGAGAAACTTTATTAAAGAAGCAAAAGTTTCTCAATTTGCCGGTTACGTATCTGAGCAGTCTACTTATCATCATGGTGAATGTTGATAAATTGATATCTACAAATGTAAGGGAAGTGCCTCTCACGTGCACAATTAAATCAGTAACTGTCCGTGGCTGCCTACTGCTTCTATATAAGGACTGATAGCCTCACGAATTAGGAGTCTTCTGTGAGCTTTGTAAAAGCCAGTTGTATAACTTGCCTCTTTTGTTCCCTGATTACATTTTGAATAAAACCTTTGTCCCTGGTGCTTTAAAGTGGATGTACACCCATTCTGGTGGAACCACTATAACCCTTTGTGTTTTGTCTCTCTTCTCTCTCTATTGCTCACTTAATACTCTGTTTCCTTAAACTTTGTTATTAACTGACTTGAAAACCCATCTCTGTCATATTAAGTAAACTGGAATTGGCACCTATAGTCCAACAGTTTGGTATCAGAGCTTGATAATAGGATTCAAGATTTGGTTGGTCGAACGCCATATCAAAGATCTTCCGCAAGAATTCAAAACCTTTGCCACTCGATTGTCGAGAATCACTGAAATCCTAACCTTGAAGAAGTTGGAGATTTCTTGGAGCATTTCACCCAATGTCAGCGAAATTTGAACTTGATGAACGATATGATGGAAAGAGCGATTTTGGACTCTGGAAACAAAAACTCAAAGCTCTCTTGATCCAACAAAAAGTTGCAAAGGTATTGTTCGATCCAGGTAAGCTTCCTTCAACTCTCTCTCTGATGATGATAAGGAAGAGATGCGAGATATCGCTCATAGCTCTATAATTCTGCATCTATCTGACAATGTTCTTAGAAGGGTAGGTAAAATTGAAAAAGTCGAAGAACTTTGGGCTAAATTAGAAGAGCTTTATTTACCTAAATCGCTTTCATGTAAAATCTTTCTAAAAGAACGTTTCTTTGGTTTCAAGATGGATCCTTCTAAGAATCTTGATGAGAATTTAGATGACTTTAACACCATATGTACTAAGTTAGAAAATTCAGGTGAAGAAGTCAAGGGAGTTGATCAAGACGTAATTCTATTAAACTCGTTACCTGAGTCTTATAAAGAAATTAAACCGGGTCTTAAATATGGCAGGGAAAAATTGTCAGTTGATATGGTACTAGATGCCCTAAGAACCAAAGAACTAGAATTAAAGAATGAGAAGAAAGATGGTGATGCTCTCTTTAGTAAAGGTAAACAGTCTAACACACAGACTCAAACTAGTAGTTCTAGAGGAAGAAATTTTAGAGGCTGAGGGAGGAACTCTCAACATAGAGGCCAACATTTTAATACCACTCAACAAACCAATAACAGGCAGCCTAGTAAAATCAATAATAGTGTAAATTCTAGTGATAAAAGTAATATAACATGTAACTATTGTCACAAAGAAGGCCACTATAAATGGGATTGTCCCCAACTAAAAAGAAAAGCTAATAAACAGAACTTCAAAGAGAACAAAAATGCAGCCAATTACCATGAAGGTTATGAGTCTGCAGAAGTTCTAGTAGCATCTGTGGCTGATTGCAAAGAGGAATGGATACTTGACTCAGGGTGTTCGTTTCATATGACCCCTTTTAGAACCTATTTTTCTGAATTTATAGAATTCGATGGAGGAAAAGTGGTCATGGGTTATCAACATTGTTTAGTTAAGGGTCTAGGGAGTGTTAAGTTGAGATTGGATGATGGTACTATGAAAATCTTGAAATCTGTAAGATATGTACCTAACTTGAAAAGAAACCTGATTTCTTTAGGGACACTTGACAAAGATGGCTGCAGGTATGAGTCTGGAAATGGCACATTAAAAGTATACAAAGGGGATTCATTAAAATTAACTGGTGTTCTGTGTAGTGGTCTGTATGTTTTGCAGGGGAAAGCAGTAGTTGGTGAACTAAATGTTGCAAATGAGCAGTTGGATAACGAAACTTATTTGTGGCACAAGAGGCTTGCACACATTAGTGAGAAAGGATTAGACTGCCTATTCAAACAAGGGCTAATTGGAAAGAAAAAACCGACTGCCTTAAGCTTTTGTGAACATTGTGTTTTGGGCAAATCAACAAGGCAAGGTTTTAAACCTGCTTCTTACACAACCAAAGACAAGCTCGAGTATGTCCATTCAGACCTTTGGGGACCAGCTCGAGTGTCCACAATTGGAGGTGCTAGGTCTTTTCTATCTTTGATTGATGACTTTTCTAGAAAACTTTGGGTTTATATGTTGAAATCAAACGATGAAGCGTTTGATCGTTTTCTAGAATGGAAAAACTTTGTAGAAAATCAAACAGGTAAACGGTTGAAATGTCTTAGGACTGACAATGGATTAGAATTTTTAAATGATAAATTTGCTAGCTTGTGTAAGTCTTCAGGAATTAGTAGGCATCTTACAGCAGCTCGAACTCCTCAGCAAAATGGACTGGCCGAAAGGTATAATAGGACAATTTTGGAAAGAGTTCGTTGTATGTTGTCCAATGCCCAGCTGCCAAGGATGTTTTGGGGTGAAGCTGTTAGCACTGCTGCCTATTTGATTAACCGCAACCCATCAACCGCTCTTGGATTCAAAACTTCTCAAGAGTTGTGGACAGGTCATGTTCCTAACTTGAAGCACCTGAAAACCTTTGGATGTGCCGCCTATGTCCACACAAGACAAGATAAGTTGCAGCCACGAGCAAAAAAATGCATTTTCATTGGTTATCCTCAAGGGGTAAAGGCATATAAGCTTTGGTGTTTAGAACTAGGGGAATCCAAGTGTATTACTAGTAGGGATGTCACCTTCAATGAATCGGATATGCCTTGGAGAACTTCTAATACAAATGGAAGTCAGCAGCAGGTTGAGACTATTTTTGAGATTGATCTTCAACCTCCCAGTGTTGGAAACTTGACTGAAACTGATGCACATCAGCAAGAAGAAATCTTAGAAGCACAAGAGCAAACTCTATTACCTAGCACATCTCAACCTATTCCAACAGACCAAGGGGAACTTGAAGACTATCAATTAACTAGAGATCGTGCAAGGAGGACAATCAGACCCTCCACAAGGTATGATTCGGATTTTGTGGGATATGCATTTAATTGTTTTACTGATAACTTTAAGTTTAAGCCTAGCACTTATAATCAAGCCATAAAATCTGAGGCTAAAGATAAGTGGATAGATGCCATGGAGCAAGAAATGCAGTCCCTACATCTTAATGAAACTTGGAAACTAGTTCAAAAACCAGCCGACAAGAAAATCATAGACTGTAAATGGATATATAAGTTGAAACCAAGTATAGATGGGCTGGATCCTCCTCGGTATAAAGCCAGACCGGTGGCTAAAGGATTTACTCAAGAAGAAGGAATCGACTACAATGAGGTATTTTCACCTGTGGTACGACATACGTCCATTCGTATTTTACTCTCCAGTGTTGTGCATTTTGGTTTAGAATTGGAACAGATGGACGTAACCACTGCCTTCTTATATGGCCACCTTGAGGAAGACATTTACATGGCTCAACCCTTAGGATTTATTGAAGAAGGAGGGGAAGACTTGGTTTGCCATCTCCAAAAATCCATTTATGGATTGAAACAATCCCCAAGGCAGTGGAATAAGAGGTTTGACACCTTTATCTTGACTCAAGGGTTTGTTAATAGTGCTTTTGATTCCTGTGTCTATAAGAAGATTGTCTCTACTGCAGTTTTTGTTTTAGTCCTTCTGTATGTAGATGATATATTAATTGCCTCTTTAGATATGCTTGAGATCTCGAAACTAAAGAGAGCTTTAAGTAAAGAGTTTAAGATGAAGGACTTAGGGGCTACCAAACGGATCCTTGGAATGGAATTAGTAAGGGACAAACAGAGAGAGACTTTGGTGATTTCTCAAACAGAATACTTCAGTAAAATTATTGCAAAATTCCATTTAGAACAAGCCAAGATAGTCAAAACTCCATTTGCTCAGCATTTCAAACTATCTGCCACCCAATCCCCAGTAAGTGAACAAGAAATAGCAGAAATGAAGACAGTTCCTTACGCAAACTTAGTGGGGAGCTTGATGTACGACATGGTGTGTTGCAGACCAGATTTAGGCAATTCTTTAAGTGTAGTCAGCCGTTACATGGGTAACCCTGGAAGAGAGCATTGGGAGGCATCTAAGTGGATAGTACGTTATCTTAAGGGAACATTGAATAAAGGTCTAATTTATTCAAAATCTGGTTCTATGACAGATATCTTGACAGGTTTTGTTGATTCGGATTATGCTGCTGACCTGGATAAACGTAGGTCTCTAACAGGTTATATTTTTACCTTATTCAATAATGTTATAAGCTGGAGGTCTGTTTTGCAATCGGTTGTAGCTTTGTCTTCAACAGAAGCCAAGTACATTGCCTTAACCGAATCAGTAAAGGAAGCTCTATGGCTGAAGGGAAGTGTATCTGAAATGCTAAATATTGAATGTGATGTAACAACTGCTCATTCCATTTTAGTATCCTGGGAAAAGCAGCAAACTCTAGAAGCTAGGACTTTATTCACCTTTGGACATGAACATTCCGTTCTCAGCGGAAACCCGCCTTAGAGATTGAACGCTACTTATCTTATTGCCATTGAACGGCCTCCTTATTCAACCAATAGAAAGAAGCCTTTGGTACAACATGTTGTAGTTAAGGTTTGGAACCCTTGCAACGCTCGGAAATAATGACTTTGTTTTATTGTGGAACAGCCGCAGGGTAAAGCTATAGTTGTCAACGATGATAGTGAAGAGGACGATTCATCTCCTGATCGATCAGAAGGTAAAGGTAAAATAATTCGCTCATTGTTGAACTACCACTGAGCATGAATTTATATATAACAAGTGATTACCAATATTAATTGGAAAGTAAATTACATTACAAAAGTTTTAACTTAGGTCCTTTTATCACATAAAACTACCACTAAACCTGAATGTTACATTGAGATTTAGATTTTCATGTAAAACTATGTTAGATTCATATTATCATGTAAAACTACCTCTAAATCAGTTCAATTACCTTGAGATTCCTGATGTTAACAGTGTAATATTAGTTTCTCTTTCTGTTTTATTATTATTTGTTTTCTGTTAGTGTTTTGTTGTGATGTAAGCCAAATGGTTAGTTCTATTGTAGCCTATTTATATCAAGGCTTGTATACTTTGTAAACAAGGAAGAATAATACAACAGCTATCATTTTCAGATTTTGTGAAAATGTTCTAATGCCCTTTGTTTCTGAAGATCATACAAATTGTTGATGATCATACTTATTTTTCATTTTTCAGTTTCAGCCATGGAAACTGAAGTGCCGGCCAAGAAAAGAGAGCAGCTGCCAAGAAGAAAACTAATGCAAAGTCTGCTCAGACCTCCAACCACGACGACAGTGACGACGAGGACTTCAAAGTAA

mRNA sequence

ATGCTCGAGTATGAGTCTTGGAAAGAGAACTTGAGAGGCAATGCAAGTGGCTGGTCAATCAAGTACTATAAGGGGTTGGGAACAAGCACATCGAAAGAAGGAAGAGAGTACTTTGATCAGCTGGAAAAACATAAGAAAGAATTTATTTGGAAAGATGCCCATGATGGAGAGGCCATTGAACTTGCGTTCAGTAAGAAGAAGATTGAAGAGAGGAAAAATTGGCTCCGTCAATTTGAGCCGCAGGGTAAAGCTATAGTTGTCAACGATGATAGTGAAGAGGACGATTCATCTCCTGATCGATCAGAAGGTAAAGTTTCAGCCATGGAAACTGAAGTGCCGGCCAAGAAAAGAGAGCAGCTGCCAAGAAGAAAACTAATGCAAAGTCTGCTCAGACCTCCAACCACGACGACAGTGACGACGAGGACTTCAAAGTAA

Coding sequence (CDS)

ATGCTCGAGTATGAGTCTTGGAAAGAGAACTTGAGAGGCAATGCAAGTGGCTGGTCAATCAAGTACTATAAGGGGTTGGGAACAAGCACATCGAAAGAAGGAAGAGAGTACTTTGATCAGCTGGAAAAACATAAGAAAGAATTTATTTGGAAAGATGCCCATGATGGAGAGGCCATTGAACTTGCGTTCAGTAAGAAGAAGATTGAAGAGAGGAAAAATTGGCTCCGTCAATTTGAGCCGCAGGGTAAAGCTATAGTTGTCAACGATGATAGTGAAGAGGACGATTCATCTCCTGATCGATCAGAAGGTAAAGTTTCAGCCATGGAAACTGAAGTGCCGGCCAAGAAAAGAGAGCAGCTGCCAAGAAGAAAACTAATGCAAAGTCTGCTCAGACCTCCAACCACGACGACAGTGACGACGAGGACTTCAAAGTAA

Protein sequence

MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIELAFSKKKIEERKNWLRQFEPQGKAIVVNDDSEEDDSSPDRSEGKVSAMETEVPAKKREQLPRRKLMQSLLRPPTTTTVTTRTSK
Homology
BLAST of Lcy01g003300 vs. ExPASy Swiss-Prot
Match: P30182 (DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 9.4e-29
Identity = 57/80 (71.25%), Postives = 67/80 (83.75%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE WKE+L+GNA+GW IKYYKGLGTST++EG+EYF  L  HKK+F+W+D  DGEAIE
Sbjct: 592 MPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIE 651

Query: 61  LAFSKKKIEERKNWLRQFEP 81
           LAFSKKKIE RKNWL  + P
Sbjct: 652 LAFSKKKIEARKNWLSSYVP 671

BLAST of Lcy01g003300 vs. ExPASy Swiss-Prot
Match: O24308 (DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.2e-25
Identity = 54/80 (67.50%), Postives = 62/80 (77.50%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WKE L  +A+ W IKYYKGLGTST +EGREYF  L +H+K+FIW D  DG AIE
Sbjct: 578 MPEYEAWKERLGNSATSWKIKYYKGLGTSTPQEGREYFSDLGRHRKDFIWDDELDGNAIE 637

Query: 61  LAFSKKKIEERKNWLRQFEP 81
           LAFSKKK E RK W+R FEP
Sbjct: 638 LAFSKKKAEGRKIWMRNFEP 657

BLAST of Lcy01g003300 vs. ExPASy Swiss-Prot
Match: P41001 (DNA topoisomerase 2 OS=Plasmodium falciparum (isolate K1 / Thailand) OX=5839 GN=TOP2 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.0e-19
Identity = 43/76 (56.58%), Postives = 56/76 (73.68%), Query Frame = 0

Query: 3   EYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIELA 62
           EYE WKEN   N  GW IKYYKGLGTST +E ++YF  ++ HK  F+W    DG++I++A
Sbjct: 631 EYEQWKENT--NLLGWKIKYYKGLGTSTDREFKQYFSDIKNHKIMFLWTGDRDGDSIDMA 690

Query: 63  FSKKKIEERKNWLRQF 79
           FSKK+IE+RK WL+ F
Sbjct: 691 FSKKRIEDRKLWLQNF 704

BLAST of Lcy01g003300 vs. ExPASy Swiss-Prot
Match: O42130 (DNA topoisomerase 2-alpha OS=Gallus gallus OX=9031 GN=TOP2A PE=2 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-16
Identity = 42/76 (55.26%), Postives = 52/76 (68.42%), Query Frame = 0

Query: 3   EYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIELA 62
           E+E WK + + N + W IKYYKGLGTSTSKE +EYF  + +H+  F +    D  AI LA
Sbjct: 595 EFEEWKSSTQ-NYNSWKIKYYKGLGTSTSKEAKEYFADMARHRIGFKYSGPEDDAAITLA 654

Query: 63  FSKKKIEERKNWLRQF 79
           FSKKK+EERK WL  F
Sbjct: 655 FSKKKVEERKEWLTNF 669

BLAST of Lcy01g003300 vs. ExPASy Swiss-Prot
Match: Q64399 (DNA topoisomerase 2-beta OS=Cricetulus longicaudatus OX=10030 GN=TOP2B PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.1e-16
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 0

Query: 3   EYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIELA 62
           E++ WK+++  N   W IKYYKGLGTST+KE +EYF  +E+H+  F +    D  AI LA
Sbjct: 603 EFDEWKKHIE-NQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLA 662

Query: 63  FSKKKIEERKNWLRQF 79
           FSKKKI++RK WL  F
Sbjct: 663 FSKKKIDDRKEWLTNF 677

BLAST of Lcy01g003300 vs. ExPASy TrEMBL
Match: A0A6J1J555 (DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.9e-34
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 588 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 647

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 648 LAFSKKKIEERKNWLRQFE 666

BLAST of Lcy01g003300 vs. ExPASy TrEMBL
Match: A0A6J1IWX9 (DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.9e-34
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 588 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 647

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 648 LAFSKKKIEERKNWLRQFE 666

BLAST of Lcy01g003300 vs. ExPASy TrEMBL
Match: A0A6J1DGT3 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.9e-34
Identity = 71/80 (88.75%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYESWKE+LRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+F+W+D HDGEAIE
Sbjct: 589 MPEYESWKESLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFEP 81
           LAFSKKKIEERKNWLR FEP
Sbjct: 649 LAFSKKKIEERKNWLRHFEP 668

BLAST of Lcy01g003300 vs. ExPASy TrEMBL
Match: A0A6J1DIB5 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.9e-34
Identity = 71/80 (88.75%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYESWKE+LRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+F+W+D HDGEAIE
Sbjct: 589 MPEYESWKESLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFEP 81
           LAFSKKKIEERKNWLR FEP
Sbjct: 649 LAFSKKKIEERKNWLRHFEP 668

BLAST of Lcy01g003300 vs. ExPASy TrEMBL
Match: A0A6J1F9E0 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.9e-34
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 589 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 649 LAFSKKKIEERKNWLRQFE 667

BLAST of Lcy01g003300 vs. NCBI nr
Match: XP_023527826.1 (DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-33
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 589 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 649 LAFSKKKIEERKNWLRQFE 667

BLAST of Lcy01g003300 vs. NCBI nr
Match: KAG7018075.1 (DNA topoisomerase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-33
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 589 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 649 LAFSKKKIEERKNWLRQFE 667

BLAST of Lcy01g003300 vs. NCBI nr
Match: XP_022935016.1 (DNA topoisomerase 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-33
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 589 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 649 LAFSKKKIEERKNWLRQFE 667

BLAST of Lcy01g003300 vs. NCBI nr
Match: XP_022982563.1 (DNA topoisomerase 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-33
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 588 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 647

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 648 LAFSKKKIEERKNWLRQFE 666

BLAST of Lcy01g003300 vs. NCBI nr
Match: XP_023527825.1 (DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-33
Identity = 71/79 (89.87%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE+WK+NLRGNASGWSIKYYKGLGTSTSKEGREYF+QL KHKK+FIW+D HDGEAIE
Sbjct: 589 MPEYEAWKDNLRGNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIE 648

Query: 61  LAFSKKKIEERKNWLRQFE 80
           LAFSKKKIEERKNWLRQFE
Sbjct: 649 LAFSKKKIEERKNWLRQFE 667

BLAST of Lcy01g003300 vs. TAIR 10
Match: AT3G23890.1 (topoisomerase II )

HSP 1 Score: 127.9 bits (320), Expect = 6.7e-30
Identity = 57/80 (71.25%), Postives = 67/80 (83.75%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE WKE+L+GNA+GW IKYYKGLGTST++EG+EYF  L  HKK+F+W+D  DGEAIE
Sbjct: 592 MPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIE 651

Query: 61  LAFSKKKIEERKNWLRQFEP 81
           LAFSKKKIE RKNWL  + P
Sbjct: 652 LAFSKKKIEARKNWLSSYVP 671

BLAST of Lcy01g003300 vs. TAIR 10
Match: AT3G23890.2 (topoisomerase II )

HSP 1 Score: 127.9 bits (320), Expect = 6.7e-30
Identity = 57/80 (71.25%), Postives = 67/80 (83.75%), Query Frame = 0

Query: 1   MLEYESWKENLRGNASGWSIKYYKGLGTSTSKEGREYFDQLEKHKKEFIWKDAHDGEAIE 60
           M EYE WKE+L+GNA+GW IKYYKGLGTST++EG+EYF  L  HKK+F+W+D  DGEAIE
Sbjct: 592 MPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIE 651

Query: 61  LAFSKKKIEERKNWLRQFEP 81
           LAFSKKKIE RKNWL  + P
Sbjct: 652 LAFSKKKIEARKNWLSSYVP 671

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P301829.4e-2971.25DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2[more]
O243082.2e-2567.50DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1[more]
P410013.0e-1956.58DNA topoisomerase 2 OS=Plasmodium falciparum (isolate K1 / Thailand) OX=5839 GN=... [more]
O421301.1e-1655.26DNA topoisomerase 2-alpha OS=Gallus gallus OX=9031 GN=TOP2A PE=2 SV=2[more]
Q643993.1e-1652.63DNA topoisomerase 2-beta OS=Cricetulus longicaudatus OX=10030 GN=TOP2B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J5555.9e-3489.87DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1[more]
A0A6J1IWX95.9e-3489.87DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1[more]
A0A6J1DGT35.9e-3488.75DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1[more]
A0A6J1DIB55.9e-3488.75DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1[more]
A0A6J1F9E05.9e-3489.87DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023527826.11.2e-3389.87DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo][more]
KAG7018075.11.2e-3389.87DNA topoisomerase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022935016.11.2e-3389.87DNA topoisomerase 2 isoform X2 [Cucurbita moschata][more]
XP_022982563.11.2e-3389.87DNA topoisomerase 2 isoform X2 [Cucurbita maxima][more]
XP_023527825.11.2e-3389.87DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G23890.16.7e-3071.25topoisomerase II [more]
AT3G23890.26.7e-3071.25topoisomerase II [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031660C-terminal associated domain of TOPRIMPFAMPF16898TOPRIM_Ccoord: 2..82
e-value: 1.8E-29
score: 102.6
NoneNo IPR availableGENE3D3.30.1490.30coord: 1..38
e-value: 3.8E-16
score: 60.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..144
NoneNo IPR availablePANTHERPTHR10169DNA TOPOISOMERASE/GYRASEcoord: 1..83
IPR013760DNA topoisomerase, type IIA-like domain superfamilySUPERFAMILY56719Type II DNA topoisomerasecoord: 3..80

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g003300.1Lcy01g003300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006265 DNA topological change
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function GO:0046872 metal ion binding